Publications by authors named "Gemma Fernández-García"

2 Publications

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Correction: Yagüe, P., et al. Goals and Challenges in Bacterial Phosphoproteomics. 2019, , 5678.

Int J Mol Sci 2020 Dec 9;21(24). Epub 2020 Dec 9.

Área de Microbiología, Departamento de Biología Funcional, IUOPA, ISPA, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain.

The authors wish to make the following corrections to this paper [1]:The author name "Gemma Fernánez-García" should be "Gemma Fernández-García" [...].
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http://dx.doi.org/10.3390/ijms21249381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764465PMC
December 2020

Cytosolic copper is a major modulator of germination, development and secondary metabolism in Streptomyces coelicolor.

Sci Rep 2019 03 12;9(1):4214. Epub 2019 Mar 12.

Área de Microbiología, Departamento de Biología Funcional, IUOPA and ISPA, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain.

Streptomycetes are important biotechnological bacteria with complex differentiation. Copper is a well-known positive regulator of differentiation and antibiotic production. However, the specific mechanisms buffering cytosolic copper and the biochemical pathways modulated by copper remain poorly understood. Here, we developed a new methodology to quantify cytosolic copper in single spores which allowed us to propose that cytosolic copper modulates asynchrony of germination. We also characterised the SCO2730/2731 copper chaperone/P-type ATPase export system. A Streptomyces coelicolor strain mutated in SCO2730/2731 shows an important delay in germination, growth and sporulation. Secondary metabolism is heavily enhanced in the mutant which is activating the production of some specific secondary metabolites during its whole developmental cycle, including germination, the exponential growth phase and the stationary stage. Forty per cent of the S. coelicolor secondary metabolite pathways, are activated in the mutant, including several predicted pathways never observed in the lab (cryptic pathways). Cytosolic copper is precisely regulated and has a pleiotropic effect in gene expression. The only way that we know to achieve the optimal concentration for secondary metabolism activation, is the mutagenesis of SCO2730/2731. The SCO2730/2731 genes are highly conserved. Their inactivation in industrial streptomycetes may contribute to enhance bioactive compound discovery and production.
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http://dx.doi.org/10.1038/s41598-019-40876-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414726PMC
March 2019