Publications by authors named "Geevaretnam Jeyasekaran"

11 Publications

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Dietary intake of trace elements from commercially important fish and shellfish of Thoothukudi along the southeast coast of India and implications for human health risk assessment.

Mar Pollut Bull 2021 Oct 9;173(Pt A):113020. Epub 2021 Oct 9.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, India.

The concentrations of eight trace elements (chromium, cobalt, copper, zinc, arsenic, cadmium, mercury and lead) in14 commercially important fish and shellfish collected from Thoothukudi along the southeast coast of India was investigated using inductively coupled plasma mass spectrometry in order to assess the health risks associated with their consumption. The concentration of trace elements ranged from 0.001 to 39.5 μg/g. The estimated weekly intake of cadmium in seven fish and shellfish (0.0081-0.0996 mg/kg body weight) were above the provisional tolerable weekly intake set by the Joint FAO/WHO Expert Committee on Food Additives. The risk assessment analysis indicated that there was non- carcinogenic risk upon lifetime consumption of rock crab, C. natator (TTHQ >1) and carcinogenic risks upon lifetime consumption of S. jello, P. semisulcatus, P. sanguinolentus C. natator, Uroteuthis duvaceli, Sepia pharaonis and Cistopus indicus due to cadmium exposure indicating a potential health risk to the exposed consumers.
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http://dx.doi.org/10.1016/j.marpolbul.2021.113020DOI Listing
October 2021

Pesticides contamination in the Thamirabarani, a perennial river in peninsular India: The first report on ecotoxicological and human health risk assessment.

Chemosphere 2021 Mar 13;267:129251. Epub 2020 Dec 13.

Tamil Nadu Fisheries University, Nagapattinam, 611002, Tamil Nadu, India.

This study evaluates the distribution of pesticides and assesses the ecological and human health risks associated with pesticide residues concentration in the Thamirabarani River, the only perennial river in Tamil Nadu, India. Observed a variation in the pesticide concentration in the water (not detected (ND)-31.69 μg/L), sediments (ND-14.77 μg/kg), and fish (0.02-26.05 μg/kg). Endosulfan, aldrin, and endrin were the predominant organochlorine pesticides present in water, sediments, and fish. The average concentration of pesticides (except endosulfan) in water and sediments was found to be below the acceptable threshold as per the water and sediment quality guidelines, posing no ecological hazard to aquatic organisms. The calculated risk quotient and toxic unit (0.1 > TU/RQ ≤ 1) represent low-to-medium acute and chronic toxicity to the aquatic organisms inhabiting the river basin. The average concentration of pesticides in fish (Labeorohita) was also below the maximum residual limits set by the Codex Alimentarius Commission (CAC). However, the calculated daily intakes of endosulfan, aldrin, and endrin were above the CAC-acceptable daily intake guidelines. The human health risk assessment showed that children and adults exposed to pesticides in water and sediments through ingestion and dermal contact could have higher cancer risks (CR > 10) than inhalation. This study recommends implementing effective and routine pollution management schemes to avoid pesticide threats to aquatic and human health.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129251DOI Listing
March 2021

A non-enzymatic electrochemical biosensor for the detection of formalin levels in fishes: Realization of a novel comparator effect based on electrolyte.

Anal Chim Acta 2020 Dec 20;1139:50-58. Epub 2020 Sep 20.

Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA Deemed University, Thanjavur, 613 401, Tamil Nadu, India; School of Electrical & Electronics Engineering, SASTRA Deemed University, Thanjavur, 613 401, Tamil Nadu, India. Electronic address:

Formalin has been used as the preservative of fishes in the concentration range of 15-25 mgL. However, there have been a high frequency of violations in the optimum use of formalin levels. The consumption of fishes treated with excessive formalin levels leads to nasopharynx, leukaemia and sinonasal cancer and there is a huge demand for the development of formalin sensor. Conventional formalin sensors such as chromogenic and mass balance sensors fall short in real-time analysis due to the lack of specificity and sensitivity in the interference medium. In this context, it has been emphasized to develop a non-enzymatic electrochemical biosensor with microwave synthesized CdS nanoparticles as a nanointerface owing to its surface limited kinetics. NaCl of 1 mM was considered as an electrolyte solution in the present study. Dynamic sensing characteristics with varying formalin levels of 5-50 mgL was studied in three different concentration ranges as 5-15 mgL (concentration of formalin < NaCl; conversion of formalin to formic acid), 20-30 mgL (concentration of formalin ∼ NaCl; equilibrium between the oxidative and reductive product), 35-50 mgL (concentration of formalin > NaCl; complete oxidation of formic acid to CO). Hence, with the exhibition of such a dynamic sensitivity based on electrolyte, the developed biosensor acts as an electrochemical comparator showcasing a switch-like behaviour in detecting formalin levels. The threshold concentration of formalin required for the comparator effect was found to be 14.845 mgL. The developed biosensor, most essentially, exhibited a versatility in quantifying formalin levels in real-time fish samples.
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http://dx.doi.org/10.1016/j.aca.2020.09.035DOI Listing
December 2020

Trace element concentrations in the organs of fish along the southeast coast of India.

Mar Pollut Bull 2021 Jan 9;162:111817. Epub 2020 Nov 9.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, Tamil Nadu, India.

Trace element pollution in the marine system is a global concern as the exposure of marine organisms to this pollution results in bioaccumulation and further transfer of the trace elements to humans through food chain. In the present study, the distribution of trace elements, namely chromium, cobalt, nickel, iron, copper, zinc, arsenic, cadmium, mercury, and lead, in gills, bone, liver, and muscle of eight commercially important fish collected along the southeast coast of India was analyzed using an inductively coupled plasma mass spectrometer. The liver was the main organ of accumulation for copper, zinc, arsenic, cadmium, and mercury; bone for chromium, cobalt, and lead; gills for copper; and muscle for arsenic and mercury. The concentration of toxic trace elements such as arsenic, cadmium, mercury, and lead in the edible portion of fish was lower than the recommended International Legislation limits, indicating that the fish of this region are safe for consumption.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111817DOI Listing
January 2021

Risk assessment of organochlorine pesticides in seaweeds along the Gulf of Mannar, Southeast India.

Mar Pollut Bull 2020 Dec 7;161(Pt B):111709. Epub 2020 Oct 7.

Department of Fisheries Economics and Statistics, Faculty of Fishery Science, Kolkata, India.

Bioaccumulation of organochlorine pesticides (OCPs) in red, green, and brown seaweeds from the Gulf of Mannar was investigated using gas chromatography-mass spectrometry (GC-MS). The concentration of OCPs in all the seaweeds was in the order of endosulfan > endrin > HCH > DDT > aldrin > heptachlor > methoxychlor. The concentrations of HCH, endrin, endosulfan, and DDT in Sargassum wightii and Gelidiella acerosa, and HCH and endosulfan in Gracilaria verrucosa were above the limits prescribed by the European Commission. However, in Ulva lactuca, except for endosulfan, all other OCPs were below the permissible level. The bioaccumulation of OCPs in seaweeds was in the order of S. wightii > G. acerosa > Gracilaria verrucosa > U. lactuca. The results of comparative analysis of the levels of the investigated pesticides against the standard human health risk assessment revealed that pesticide exposure from seaweeds is hazardous to human health and the marine environment of the Gulf of Mannar.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111709DOI Listing
December 2020

Human health risk assessment of heavy metals in aquatic sediments and freshwater fish caught from Thamirabarani River, the Western Ghats of South Tamil Nadu.

Mar Pollut Bull 2020 Oct 29;159:111496. Epub 2020 Jul 29.

Tamil Nadu Fisheries University, Nagapattinam 611 002, Tamil Nadu, India.

Industrialization and advancements in agriculture are increasingly the cause of environmental concerns and need to be addressed. This study was carried out at four sampling sites, viz. Manimuthar (site 1), Tirunelveli (site 2), Srivaikuntam (site 3), and Authoor (site 4), along the Thamirabarani river. The heavy metal concentration in fish and sediments were ranged from 0.001 to 9.505 mg kg and 0.294 to 106.25 mg kg. The lifetime cancer risk (LCR) values for children and adults were found to be above the acceptable threshold value (ATV) and children were found to be more susceptible to health risks. For all sites except site 4, the LCR of toxic metals except Cd were within the ATV (10-10); the value of Cd was above the ATV, and poses a high cancer risk to the downstream inhabitants. This study advises routine heavy metal monitoring on aquatic organisms along these sites to implement regulatory standards.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111496DOI Listing
October 2020

Prevalence of antibiotic resistant Salmonella spp. strains in shrimp farm source waters of Nagapattinam region in South India.

Mar Pollut Bull 2020 Jun 28;155:111171. Epub 2020 Apr 28.

Department of Fishing Technology & Fisheries Engineering, FC&RI, TNJFU, Thoothukudi 628008, India.

The prevalence of antibiotic-resistant Salmonella spp. in the source waters of shrimp farms in the Nagapattinam region of South India was investigated. Water and sediment samples (188 Nos.) collected from 23 natural creeks during December 2018 to April 2019 were examined for Salmonella spp. by conventional and PCR methods. The study indicated 28.7% of water and 25.5% of sediment isolates as Salmonella spp., while PCR test gave positive for 7.44% and 5.15% of the isolates, respectively. The isolates were resistant to sulfonamide (SF), but sensitive to tetracycline (TC), chloramphenicol (CAP), and furazolidone (FZ). PCR amplification of mitochondrial 16S rRNA region identified the highly resistant Salmonella serovar as S. Montevideo, which is an emerging food-borne pathogen. The incidence of antibiotic-resistant S. Montevideo reported for the first time in the natural creeks that supply water for shrimp farms emphasizes the need for regulatory steps to control its prevalence.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111171DOI Listing
June 2020

Concentrations of trace elements in the organs of commercially exploited crustaceans and cephalopods caught in the waters of Thoothukudi, South India.

Mar Pollut Bull 2020 May 11;154:111045. Epub 2020 Mar 11.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, Tamil Nadu, India.

Concentrations of trace elements (arsenic, cadmium, mercury, and lead) in tissues (muscle, gills, and digestive gland) of three commercially exploited crustaceans (Portunus sanguinolentus, Charybdis natator, and Penaeus semisulcatus) and three cephalopods (Doryteuthis sibogae, Sepia pharaonis, and Cistopus indicus) were examined. The animals were captured in the waters of Thoothukudi, and the tissues of six individuals of each species were analyzed using ICP-MS. The highest concentrations of arsenic (16.5 μg/g) and mercury (0.052 μg/g) were recorded in the digestive gland of C. natator, and cadmium (69.9 μg/g) and lead (0.351 μg/g) in the digestive gland of S. pharaonis. The edible portion of the crustaceans and cephalopods contained lower concentrations of trace elements, and these were below allowable limits set by the European Union. The edible parts of the investigated samples are safe for human consumption, but accidental contamination of the edible tissues with material from the digestive glands could lead to concerns relating to metal toxicity.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111045DOI Listing
May 2020

A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNA region to authenticate four commercially important shrimp species in India.

Food Chem 2018 Jan 23;239:369-376. Epub 2017 Jun 23.

Department of Fisheries Environment, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukkudi 628 008, India.

Food authenticity is an issue of major concern for food authorities, as mislabeling represents one of the major commercial frauds. In this study, a novel PCR-RFLP protocol was developed as a tool to authenticate four shrimp products of commercial importance belonging to the family, Penaeidae, viz. Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus. PCR amplification was performed targeting 16S rRNA/tRNA region having an amplicon size of 530bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3. Subsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded distinct RFLP pattern for each species of shrimps having fragment sizes below 150bp. The unique RFLP patterns were also obtained in processed shrimp products without any degradation or alteration in the major fragments. The method was also validated with commercial shrimp products. Thus, the developed protocol can be performed within 8h using a single enzyme to authenticate four shrimp products of commercial significance.
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http://dx.doi.org/10.1016/j.foodchem.2017.06.132DOI Listing
January 2018

Antioxidant activities of squid protein hydrolysates prepared with papain using response surface methodology.

Food Sci Biotechnol 2016 30;25(3):665-672. Epub 2016 Jun 30.

2Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukkudi, 628008 India.

Squid protein hydrolysates (SPH) were prepared from the Indian squid using papain. Response surface methodology (RSM) was used for optimization of hydrolysis conditions, including temperature, time, and the enzyme-substrate ratio using DPPH radical scavenging activity as a response. The amino acid composition of SPH was compared with raw squid muscle. antioxidant activities were evaluated based on reducing power, metal chelation, ABTS, hydroxyl radical, and superoxide anion radical scavenging assays. SPH exhibited good ABTS radical scavenging activities of 96.50±0.90%, superoxide anion radical scavenging activities of 96.4±0.89%, reducing powers of 0.71±0.02, moderate hydroxyl radical scavenging activities of 64.03±2.11%, and metal chelating activities of 52.04±1.02%. antioxidant activities determined using a sardine minced model system showed 42% reduction in formation of secondary oxidative products as thiobarbituric acid reactive substances (TBARS), almost equivalent to reduction by ascorbic acid of 41.42% at 400 ppm.
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http://dx.doi.org/10.1007/s10068-016-0117-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049156PMC
June 2016

Multiplex polymerase chain reaction-based assay for the specific detection of toxin-producing Vibrio cholerae in fish and fishery products.

Appl Microbiol Biotechnol 2011 May 1;90(3):1111-8. Epub 2011 Mar 1.

Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin 628 008, India.

A multiplex polymerase chain reaction (MPCR)-based assay was developed for the simultaneous detection of Vibrios using the genus-specific RNA polymerase subunit A (rpoA) gene and specific detection of toxin-producing Vibrio cholerae strains using two sets of primer based on cholera toxin subunit A (ctxA) and repeat in toxin subunit A (RtxA)-producing genes. The MPCR method developed is applicable to both the simultaneous and the two-step detection of genus Vibrio total and toxigenic V. cholerae species. This assay was specific as no amplification occurred with the other bacterial pathogens tested. The sensitivity of the assay was tested by artificially spiking the shrimp homogenate with the toxigenic strain of V. cholerae (NICED 16582) in different dilutions. The developed MPCR assay could detect three cells of V. cholerae in 12 h pre-enrichment in APW. The proposed method is rapid, sensitive, and specific for the detection of Vibrio genus as well as toxin-producing V. cholerae strains in environmental samples.
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http://dx.doi.org/10.1007/s00253-011-3175-9DOI Listing
May 2011
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