Publications by authors named "Ge Li"

614 Publications

Novel rhino-like SHJH mice with thyroid dysfunction.

Zool Res 2021 Nov;42(6):734-738

Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong 510663, China.

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http://dx.doi.org/10.24272/j.issn.2095-8137.2021.211DOI Listing
November 2021

Accuracy of FibroTouch in assessing liver steatosis and fibrosis in patients with metabolic-associated fatty liver disease combined with type 2 diabetes mellitus.

Ann Palliat Med 2021 Sep;10(9):9702-9714

Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Artificial Cell Engineering Technology Research Center, Tianjin Institute of Hepatobiliary Disease, The Third Central Hospital of Tianjin, Tianjin, China; Department of Gastroenterology and Hepatology, The Third Central Clinical College of Tianjin Medical University, Tianjin, China.

Background: Metabolic-associated fatty liver disease (MAFLD) is highly prevalent in type 2 diabetes mellitus (T2DM) patients and can rapidly progress to steatohepatitis, liver fibrosis, and hepatocellular carcinoma (HCC). Accurate evaluation and proper management of MAFLD can help prevent adverse liver outcomes. Here we evaluated the precision of the FibroTouch (FT) in the staging of liver steatosis and fibrosis in patients with MAFLD combined with T2DM using two indicators: controlled attenuation parameter (CAP) and liver stiffness measurement (LSM).

Methods: Eighty-five adult MAFLD combined with T2DM patients were selected at our center from July 2016 to July 2019 and underwent liver puncture biopsy for histopathology and the FT assay simultaneously. Two blinded pathologists independently reviewed the samples. The severity of fatty liver was classified using two scoring systems: the nonalcoholic fatty liver disease activity score (NAS) and the fibrosis score. Scores were then assessed following the Pathology Working Group of the NASH Clinical Research Network of the National Institutes of Health. Similarly, the severity of nonalcoholic steatohepatitis (NASH) was classified using the European Steatosis Activity Fibrosis (SAF) system. The FT assay was applied to obtain the LSM and the CAP. FT accuracy in diagnosing steatosis and fibrosis was determined by the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUROC).

Results: Using biopsy analysis as the gold standard, the AUROCs and cutoff values of CAP in diagnosing liver steatosis were as follows: 0.84 (95% CI: 0.67-1.01) and 278 dB/m for S ≥ S1, 0.88 (95% CI: 0.81-0.95) and 305 dB/m for S ≥ S2, 0.89 (95% CI: 0.82-0.95) and 307 dB/m for S ≥ S3. The AUROCs and cutoff values of LSM in diagnosing liver fibrosis were as follows: 0.76 (95% CI: 0.66-0.86) for F ≥ F2, 0.81 (95% CI: 0.71-0.91) and 13.8 kPa for F ≥ F3, 0.92 (95% CI: 0.85-1.00) and 20.1 kPa for F ≥ F4.

Conclusions: In patients of MAFLD with T2DM, CAP and LSM obtained by FT are highly accurate in assess liver steatosis and fibrosis, respectively, with AUROC values ranging from 0.76 to 0.92.
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http://dx.doi.org/10.21037/apm-21-2339DOI Listing
September 2021

Research ability and research motivation of postgraduate nursing students in traditional Chinese medicine colleges.

Nurs Open 2021 Oct 3. Epub 2021 Oct 3.

School of Nursing, Fujian University of Traditional Chinese Medicine, Fuzhou, China.

Aim: To investigate the relationship between research ability and research motivation of postgraduate nursing students in traditional Chinese medicine colleges and identify other factors that may have an impact on the research ability of postgraduate nursing students.

Design: A cross-sectional electronic survey was used to collect data from 191 postgraduate nursing students.

Methods: A total of 191 postgraduate nursing students from seven traditional Chinese medicine colleges were investigated from October to November 2020 using self-rated scales for research ability and research motivation. The relationship between the variables affecting the research ability of postgraduate nursing students in traditional Chinese medicine colleges was determined.

Results: There was a positive correlation between the score of self-rated research ability and research motivation among 191 postgraduate nursing students in traditional Chinese medicine colleges. Multiple stepwise regression analysis showed that grade, research motivation, age and active participation in class discussions were the main factors affecting the self-rated research ability.

Conclusion: The self-rated research ability of postgraduate nursing students in traditional Chinese medicine colleges is positively correlated with research motivation. According to the research motivation orientation, adopting targeted training methods and establishing correct professional understanding may improve the research ability of postgraduate nursing students.
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http://dx.doi.org/10.1002/nop2.1079DOI Listing
October 2021

Cerebrospinal Fluid MicroRNA Changes in Cognitively Normal Veterans With a History of Deployment-Associated Mild Traumatic Brain Injury.

Front Neurosci 2021 9;15:720778. Epub 2021 Sep 9.

Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, OR, United States.

A history of traumatic brain injury (TBI) increases the odds of developing Alzheimer's disease (AD). The long latent period between injury and dementia makes it difficult to study molecular changes initiated by TBI that may increase the risk of developing AD. MicroRNA (miRNA) levels are altered in TBI at acute times post-injury (<4 weeks), and in AD. We hypothesized that miRNA levels in cerebrospinal fluid (CSF) following TBI in veterans may be indicative of increased risk for developing AD. Our population of interest is cognitively normal veterans with a history of one or more mild TBI (mTBI) at a chronic time following TBI. We measured miRNA levels in CSF from three groups of participants: (1) community controls with no lifetime history of TBI (ComC); (2) deployed Iraq/Afghanistan veterans with no lifetime history of TBI (DepC), and (3) deployed Iraq/Afghanistan veterans with a history of repetitive blast mTBI (DepTBI). CSF samples were collected at the baseline visit in a longitudinal, multimodal assessment of Gulf War veterans, and represent a heterogenous group of male veterans and community controls. The average time since the last blast mTBI experienced was 4.7 ± 2.2 years [1.5 - 11.5]. Statistical analysis of TaqMan miRNA array data revealed 18 miRNAs with significant differential expression in the group comparisons: 10 between DepTBI and ComC, 7 between DepC and ComC, and 8 between DepTBI and DepC. We also identified 8 miRNAs with significant differential detection in the group comparisons: 5 in DepTBI vs. ComC, 3 in DepC vs. ComC, and 2 in DepTBI vs. DepC. When we applied our previously developed multivariable dependence analysis, we found 13 miRNAs (6 of which are altered in levels or detection) that show dependencies with participant phenotypes, e.g., ApoE. Target prediction and pathway analysis with miRNAs differentially expressed in DepTBI vs. either DepC or ComC identified canonical pathways highly relevant to TBI including senescence and ephrin receptor signaling, respectively. This study shows that both TBI and deployment result in persistent changes in CSF miRNA levels that are relevant to known miRNA-mediated AD pathology, and which may reflect early events in AD.
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http://dx.doi.org/10.3389/fnins.2021.720778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463659PMC
September 2021

HO-Mediated Oxidative Stress Enhances Cystathionine γ-Lyase-Derived HS Synthesis via a Sulfenic Acid Intermediate.

Antioxidants (Basel) 2021 Sep 18;10(9). Epub 2021 Sep 18.

School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.

Hydrogen sulfide (HS), which is generated mainly by cystathionine -lyase (CSE) in the cardiovascular system, plays a pivotal role in a wide range of physiological and pathological processes. However, the regulatory mechanism of the CSE/HS system is poorly understood. Herein, we show that oxidation induces the disulfide bond formation between Cys252 and Cys255 in the CXXC motif, thus stimulating the HS-producing activity of CSE. The activity of oxidized CSE is approximately 2.5 fold greater than that of the reduced enzyme. Molecular dynamics and molecular docking suggest that the disulfide bond formation induces the conformational change in the active site of CSE and consequently increases the affinity of the enzyme for the substrate L-cysteine. Mass spectrometry and mutagenesis studies further established that the residue Cys255 is crucial for oxidation sensing. Oxidative stress-mediated sulfenylation of Cys255 leads to a sulfenic acid intermediate that spontaneously forms an intramolecular disulfide bond with the vicinal thiol group of Cys252. Moreover, we demonstrate that exogenous hydrogen peroxide (HO) and endogenous HO triggered by vascular endothelial growth factor (VEGF) promote cellular HS production through the enhancement of CSE activity under oxidative stress conditions. By contrast, incubation with HO or VEGF did not significantly enhance cellular HS production in the presence of PEG-catalase, an enzymatic cell-permeable HO scavenger with high HO specificity. Taken together, we report a new posttranslational modification of CSE that provides a molecular mechanism for HO/HS crosstalk in cells under oxidative stress.
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http://dx.doi.org/10.3390/antiox10091488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466214PMC
September 2021

Effects of fertilizer under different dripline spacings on summer maize in northern China.

Sci Rep 2021 Sep 23;11(1):18922. Epub 2021 Sep 23.

Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/ Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 South Road, Zhongguancun, Haidian District, Beijing, 100081, People's Republic of China.

Maximizing grain yields with effective fertilization technologies and minimizing nitrogen losses is essential in agroecosystems. In this research, we conducted a two-year field experiment to explore whether dripline spacing and fertilization rate would affect maize grain yield. Two dripline spacings (i.e., one drip line per row of maize with a row space of 60 cm and one drip line per two rows of maize) and two fertilization rates (i.e., high fertilization level: N, 180 kg ha; PO, 90 kg ha; and KO, 90 kg ha and low level: N, 139.5 kg ha; PO, 76.5 kg ha; and KO, 76.5 kg ha) were employed in this research. The results showed that maize yield was significantly affected by both dripline spacing and fertilization rate. The maize yield was 10.2% higher in the treatment with one drip line per two rows than that in the treatment with one drip line per row. Maize yield increased by 10.9% at the high fertilization level compared to that at the low fertilization level. The quantity of cumulative ammonia volatilization was reduced by 15.1% with one drip line per two rows compared to that with one drip line per row, whereas it increased by 26.9% at the high fertilization level compared with that at the low fertilization level. These results indicated that one drip line per two rows with a high fertilization rate increased the yield and could reduce the environmental burden, which may be economically beneficial and environmentally sound for maize fertigation for green agricultural development.
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http://dx.doi.org/10.1038/s41598-021-98016-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460782PMC
September 2021

Myeloid neddylation targets IRF7 and promotes host innate immunity against RNA viruses.

PLoS Pathog 2021 Sep 10;17(9):e1009901. Epub 2021 Sep 10.

Beijing Institute of Basic Medical Sciences, Beijing, China.

Neddylation, an important type of post-translational modification, has been implicated in innate and adapted immunity. But the role of neddylation in innate immune response against RNA viruses remains elusive. Here we report that neddylation promotes RNA virus-induced type I IFN production, especially IFN-α. More importantly, myeloid deficiency of UBA3 or NEDD8 renders mice less resistant to RNA virus infection. Neddylation is essential for RNA virus-triggered activation of Ifna gene promoters. Further exploration has revealed that mammalian IRF7undergoes neddylation, which is enhanced after RNA virus infection. Even though neddylation blockade does not hinder RNA virus-triggered IRF7 expression, IRF7 mutant defective in neddylation exhibits reduced ability to activate Ifna gene promoters. Neddylation blockade impedes RNA virus-induced IRF7 nuclear translocation without hindering its phosphorylation and dimerization with IRF3. By contrast, IRF7 mutant defective in neddylation shows enhanced dimerization with IRF5, an Ifna repressor when interacting with IRF7. In conclusion, our data demonstrate that myeloid neddylation contributes to host anti-viral innate immunity through targeting IRF7 and promoting its transcriptional activity.
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http://dx.doi.org/10.1371/journal.ppat.1009901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432861PMC
September 2021

RGD-functionalised melanin nanoparticles for intraoperative photoacoustic imaging-guided breast cancer surgery.

Eur J Nucl Med Mol Imaging 2021 Sep 10. Epub 2021 Sep 10.

Cancer Center & Department of Breast and Thyroid Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, 2000 Xiang'an Road East, Xiamen, 361101, Fujian, China.

Purpose: Obtaining tumour-free margins is critical for avoiding re-excision and reducing local recurrence following breast-conserving surgery; however, it remains challenging. Imaging-guided surgery provides precise detection of residual lesions and assists surgical resection. Herein, we described water-soluble melanin nanoparticles (MNPs) conjugated with cyclic Arg-Gly-Asp (cRGD) peptides for breast cancer photoacoustic imaging (PAI) and surgical navigation.

Methods: The cRGD-MNPs were synthesised and characterized for morphology, photoacoustic characteristics and stability. Tumour targeting and toxicity of cRGD-MNPs were determined by using either breast cancer cells, MDA-MB-231 tumour-bearing mice or the FVB/N-Tg (MMTV-PyVT) 634Mul/J mice model. PAI was used to locate the tumour and guide surgical resection in MDA-MB-231 tumour-bearing mice.

Results: The cRGD-MNPs exhibited excellent in vitro and in vivo tumour targeting with low toxicity. Intravenous administration of cRGD-MNPs to MDA-MB-231 tumour-bearing mice showed an approximately 2.1-fold enhancement in photoacoustic (PA) intensity at 2 h, and the ratio of the PA intensity at the tumour site to that in the surrounding normal tissue was 3.2 ± 0.1, which was higher than that using MNPs (1.7 ± 0.3). Similarly, the PA signal in the spontaneous breast cancer increased ~ 2.5-fold at 2 h post-injection of cRGD-MNPs in MMTV-PyVT transgenic mice. Preoperative PAI assessed tumour volume and offered three-dimensional (3D) reconstruction images for accurate surgical planning. Surgical resection following real-time PAI showed high consistency with histopathological analysis.

Conclusion: These results highlight that cRGD-MNP-mediated PAI provide a powerful tool for breast cancer imaging and precise tumour resection. cRGD-MNPs with fine PA properties have great potential for clinical translation.
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http://dx.doi.org/10.1007/s00259-021-05545-3DOI Listing
September 2021

Dynamic environmental regulation threshold effect of technical progress on green total factor energy efficiency: evidence from China.

Authors:
Ge Li Da Gao Yi Li

Environ Sci Pollut Res Int 2021 Sep 7. Epub 2021 Sep 7.

School of Economics, Huazhong University of Science and Technology, No. 1037, Luoyu Road, Hongshan District, Wuhan, Hubei, People's Republic of China.

Sustainability is a strategic choice for the transition to a green economy in China. Improving green total factor energy efficiency (GTFEE) is the key to realizing the dual targets of energy-saving and economic growth. This paper empirically tests the nonlinear effects of environmental regulation on technical innovation affecting GTFEE by using panel data of 271 prefecture-level cities in China from 2004 to 2019. Meanwhile, a system GMM approach is used to verify the channels through which technical innovation affects GTFEE. Finally, the spatial and temporal characteristics of technical innovation affecting GTFEE are analyzed from the perspective of environmental regulation. The empirical results are as follows: (1) Technical innovation can significantly improve GTFEE. However, this improvement effect is a threshold characteristic; when environmental regulation is above the threshold value, technical innovation has a greater improvement effect on GTFEE. (2) Besides directly influencing GTFEE, technical innovation can also indirectly influence GTFEE via channels such as economic growth effect, industrial structure upgrading effect, and foreign investment effect. Meanwhile, indirect influence channels also show environmental regulation heterogeneity. (3) The number of cities crossing the threshold of environmental regulation in China increases year by year, which helps technical innovation play a role in improving GTFEE. However, there are still a small number of cities that do not cross the threshold during the sample period, which should attract the attention of local governments.
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http://dx.doi.org/10.1007/s11356-021-16292-1DOI Listing
September 2021

Cross-fostering alleviates depression-like behavior mediated by EAAT2 and SNARE complex in prenatal stress offspring rat.

Pharmacol Biochem Behav 2021 Nov 4;210:173269. Epub 2021 Sep 4.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Institute of Maternal and Infant Health, Northwest University, Xi'an 710069, Shaanxi, China. Electronic address:

Previous studies have shown that prenatal stress (PS) can potentially contribute to depression-like behavior in offspring and that this effect may be moderated by cross-fostering. However, the underlying mechanism of this effect remains to be determined. This study aimed to determine the effect of cross-fostering on the expression of EAAT2 and the SNARE complex in the hippocampus and the prefrontal cortex of PS offspring rats and to demonstrate functional effects on depression-like behavior. The impacts of cross-fostering were functionally assessed using the sucrose preference test (SPT), the forced swimming test (FST) and the elevated plus maze (EPM). Quantitative real-time PCR was used to determine changes in the expression of EAAT2 and SNAREs mRNA in the hippocampus and the prefrontal cortex of offspring rats. PS offspring rats showed significantly decreased sucrose preference and prolonged immobility time, while cross-fostering effectively increased sucrose preference and shorten the time of immobility. The expression of EAAT2 mRNA in PS offspring rats was markedly reduced, whilst the core mRNA expression of the SNARE complex increased. Our results provide strong evidence demonstrating that cross-fostering can alleviate depression-like behavior and regulate the abnormal expression levels of EAAT2 mRNA and SNARE complex in the hippocampus and the prefrontal cortex of PS offspring rats. Our findings contribute to further understanding of the pathogenesis of PS-induced depression and may help to inform the future development of novel treatment approaches.
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http://dx.doi.org/10.1016/j.pbb.2021.173269DOI Listing
November 2021

Mesenchymal stem cell-derived exosome microRNA as therapy for cardiac ischemic injury.

Biomed Pharmacother 2021 Nov 1;143:112118. Epub 2021 Sep 1.

Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China. Electronic address:

Cardiovascular diseases (CVD) are a significant cause of human health harm. In the past, stem cell therapy was reported to have functional defects, such as immune rejection, tumorigenicity, and infusion toxicity. Exosomes are extracellular vesicles with lipid bilayer membrane structure, containing proteins, lipids, mRNA, miRNA, DNA, and other molecules, which can mediate various biological functions such as immune response, inflammatory response, cell migration, and differentiation intercellular communication. Exosomal miRNAs have outstanding advantages in disease diagnosis and curative effect prediction. Likewise, paracrine factors could also mediate the main therapeutic effect of mesenchymal stem cells. Research has shown that mesenchymal stem cell-derived micro-exosomes, which may come from stem cells, accumulate in the ischemic tissue and regulate cell proliferation, apoptosis, inflammation, and angiogenesis sites of myocardial injury after being transplanted. This review reviewed the molecular mechanisms of exosomes and internal microRNAs derived from mesenchymal stem cells in cardiac ischemic injury repair.
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http://dx.doi.org/10.1016/j.biopha.2021.112118DOI Listing
November 2021

Molecular mechanism of Epicedium treatment for depression based on network pharmacology and molecular docking technology.

BMC Complement Med Ther 2021 Sep 3;21(1):222. Epub 2021 Sep 3.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Institute of Maternal and Infant health, Northwest University, Xi'an, 710069, Shanxi Province, China.

Background: Increasing attention has been paid to the effect of Epimedium on the nervous system, particularly anti-depression function. In the present study, we applied network pharmacology to introduce a testable hypothesis on the multi-target mechanisms of Epicedium against depression.

Methods: By reconstructing the network of protein-protein interaction and drug-component-target, we predicted the key protein targets of Epicedium for the treatment of depression. Then, through molecular docking, the interaction of the main active components of Epicedium and predicted candidate targets were verified.

Results: Nineteen active compounds were selected from Epicedium. There were 200 targets associated with Epicedium and 537 targets related to depression. The key targets of Epicedium for treating depression were IL6, VEGFA, AKT1, and EGF. According to gene ontology functional enrichment analysis, 22 items of biological process (BP), 13 items of cell composition (CC) and 9 items of molecular function (MF) were obtained. A total of 56 signaling pathways (P < 0.05) were identified by Kyoto Encyclopedia of Genes and Genomes analysis, mainly involving depression-related pathways such as dopaminergic synapse, TNF signaling pathway, and prolactin signaling pathway. The results of molecular docking showed that the most important activity components, including luteoklin, quercetin and kaempferol, were well combined with the key targets.

Conclusions: Luteoklin, quercetin, kaempferol and other active compounds in Epicedium can regulate multiple signaling pathways and targets such as IL6, AKT1, and EGF, therefore playing therapeutic roles in depression.
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http://dx.doi.org/10.1186/s12906-021-03389-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417989PMC
September 2021

Functional homoeologous alleles of CONSTANS contribute to seasonal crop type in rapeseed.

Theor Appl Genet 2021 Oct 19;134(10):3287-3303. Epub 2021 Aug 19.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

Key Message: Two CO paralogs in Brassica napus were confirmed and shown distinct expression pattern and function in promoting flowering and allelic variation s within BnaCO.A10 were found closely associated with ecotype divergence. CONSTANS (CO) is a key gene that responds to photoperiod and in Arabidopsis can promote flowering under long-day (LD) conditions. Brassica napus L. is a major oil crop and close relative of Arabidopsis, and arose via allopolyploidization from the diploids B. rapa (A genome) and B. oleracea (C genome). In this study, we confirmed that B. napus has two CO genes located on the A10 (BnaCO.A10) and C9 (BnaCO.C9) chromosomes. Significant differences in level and temporal pattern of transcription, as well as in protein function, of these homoeologous may have resulted from sequence variation in the promoter as well as in the coding region. Apart from two insertions of 527 bp and 2002 bp in the promoter of BnaCO.C9 that function as transcriptional enhancers, this gene is otherwise highly conserved in both promoter and coding region. However, BnaCO.A10 was classified into two haplotypes and transgene analysis in Arabidopsis and backcross analysis in rapeseed indicated that the winter-type haplotype had a greater effect in promoting flowering than the spring type. We discuss the contribution of CO alleles to species evolution, and for eco-geographic radiation following crop domestication, alongside scope for managing this locus in future breeding.
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http://dx.doi.org/10.1007/s00122-021-03896-xDOI Listing
October 2021

In Situ Synthesis of Robust Polyvinylpyrrolidone-Based Perovskite Nanocrystal Powders by the Fiber-Spinning Chemistry Method and Their Versatile 3D Printing Patterns.

ACS Appl Mater Interfaces 2021 Aug 12;13(33):39748-39754. Epub 2021 Aug 12.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Jiangsu Key Laboratory of Fine Chemicals and Functional Polymer Materials, Nanjing Tech University, No. 5 Xin Mofan Road, Nanjing 210009, P. R. China.

All-inorganic halide perovskite nanocrystals (PNCs) have received increasing attention due to their excellent optical properties. However, the inherent instability and the large amount of volatile organic compounds during the production process have severely limited their applications. In this research, we employed the microfluidic electrostatic spinning method to synthesize polyvinylpyrrolidone (PVP)-based PNC (CsPbBr/PVP) powders directly by spinning chemistry, where the fibers serve as reactors. Thus, 20.1 g of CsPbBr/PVP powders was obtained, which exhibits good fluorescent properties and high stability. Based on these excellent properties, several new applications were explored, including 3D printing, direct encapsulants for light-emitting diodes, and fluorescent coatings. It should be noted that the powder showed distinct advantages in 3D printing, allowing the fabrication of a series of fluorescent patterns, which offers a new candidate for fluorescent 3D printable materials. This work not only opens up an optimal way for facile production of fluorescent powders by the spinning chemistry strategy, but also provides a new perspective for various application directions, especially for 3D printing.
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http://dx.doi.org/10.1021/acsami.1c10806DOI Listing
August 2021

Probiotic supplements alleviate gestational diabetes mellitus by restoring the diversity of gut microbiota: a study based on 16S rRNA sequencing.

J Microbiol 2021 Sep 12;59(9):827-839. Epub 2021 Aug 12.

Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, P. R. China.

Probiotics effectively prevent and improve metabolic diseases such as diabetes by regulating the intestinal microenvironment and gut microbiota. However, the effects of probiotics in gestational diabetes mellitus are not clear. Here, we showed that probiotic supplements significantly improved fasting blood glucose in a gestational diabetes mellitus rat model. To further understand the mechanisms of probiotics in gestational diabetes mellitus, the gut microbiota were analyzed via 16S rRNA sequencing. We found that compared with the normal pregnant group, the gestational diabetes mellitus rats had decreased diversity of gut microbiota. Moreover, probiotic supplementation restored the diversity of the gut microbiota in gestational diabetes mellitus rats, and the gut microbiota structure tended to be similar to that of normal pregnant rats. In particular, compared with gestational diabetes mellitus rats, the abundance of Firmicutes and Actinobacteria was higher after probiotic supplementation. Furthermore, activating carbohydrate metabolism and membrane transport pathways may be involved in the potential mechanisms by which probiotic supplements alleviate gestational diabetes mellitus. Overall, our results suggested that probiotic supplementation might be a novel approach to restore the gut microbiota of gestational diabetes mellitus rats and provided an experimental evidence for the use of probiotic supplements to treat gestational diabetes mellitus.
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http://dx.doi.org/10.1007/s12275-021-1094-8DOI Listing
September 2021

Folate receptor-targeting semiconducting polymer dots hybrid mesoporous silica nanoparticles against rheumatoid arthritis through synergistic photothermal therapy, photodynamic therapy, and chemotherapy.

Int J Pharm 2021 Sep 3;607:120947. Epub 2021 Aug 3.

School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, Jilin 130012, China. Electronic address:

With ideal optical properties, semiconducting polymer quantum dots (SPs) have become a research focus in recent years; a considerable number of studies have been devoted to the application of SPs in non-invasive and biosafety phototherapy with near-infrared (NIR) lasers. Nevertheless, the relatively poor stability of SPs in vitro and in vivo remains problematic. PCPDTBT was chosen to synthesize photothermal therapy (PTT) and photodynamic therapy (PDT) dual-model SPs, considering its low band gap and desirable absorption in the NIR window. For the first time, cetrimonium bromide was used as a stabilizer to guarantee the in vitro stability of SPs, and as a template to prepare SP hybrid mesoporous silica nanoparticles (SMs) to achieve long-term stability in vivo. The mesoporous structure of SMs was used as a reservoir for the hypoxia-activated prodrug Tirapazamine (TPZ). SMs were decorated with polyethylene glycol-folic acid (SMPFs) to specifically target activated macrophages in rheumatoid arthritis (RA). Upon an 808 nm NIR irradiation, the SMPFs generate intracellular hyperthermia and excessive singlet oxygen. Local hypoxia caused by molecular oxygen consumption simultaneously activates the cytotoxicity of TPZ, which effectively kills activated macrophages and inhibits the progression of arthritis. This triple PTT-PDT-chemo synergistic treatment suggests that SMPFs realize the in vivo application of SPs and may be a potential nano-vehicle for RA therapy with negligible side-toxicity.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120947DOI Listing
September 2021

Fine Particulate Matter and Dementia Incidence in the Adult Changes in Thought Study.

Environ Health Perspect 2021 Aug 4;129(8):87001. Epub 2021 Aug 4.

Department of Environmental and Occupational Health Sciences, University of Washington Seattle School of Public Health, Seattle, Washington, USA.

Background: Air pollution may be associated with elevated dementia risk. Prior research has limitations that may affect reliability, and no studies have evaluated this question in a population-based cohort of men and women in the United States.

Objectives: We evaluated the association between time-varying, 10-y average fine particulate matter () exposure and hazard of all-cause dementia. An additional goal was to understand how to adequately control for age and calendar-time-related confounding through choice of the time axis and covariate adjustment.

Methods: Using the Adult Changes in Thought (ACT) population-based prospective cohort study in Seattle, we linked spatiotemporal model-based exposures to participant addresses from 1978 to 2018. Dementia diagnoses were made using high-quality, standardized, consensus-based protocols at biennial follow-ups. We conducted multivariable Cox proportional hazards regression to evaluate the association between time-varying, 10-y average exposure and time to event in a model with age as the time axis, stratified by apolipoprotein E (APOE) genotype, and adjusted for sex, education, race, neighborhood median household income, and calendar time. Alternative models used calendar time as the time axis.

Results: We report 1,136 cases of incident dementia among 4,166 individuals with nonmissing APOE status. Mean [mean ± standard deviation (SD)] 10-y average was . Each increase in the moving average of 10-y was associated with a 16% greater hazard of all-cause dementia [1.16 (95% confidence interval: 1.03, 1.31)]. Results using calendar time as the time axis were similar.

Discussion: In this prospective cohort study with extensive exposure data and consensus-based outcome ascertainment, elevated long-term exposure to was associated with increased hazard of all-cause dementia. We found that optimal control of age and time confounding could be achieved through use of either age or calendar time as the time axis in our study. Our results strengthen evidence on the neurodegenerative effects of . https://doi.org/10.1289/EHP9018.
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http://dx.doi.org/10.1289/EHP9018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336685PMC
August 2021

Research on multiple bubbles in China's multi-level stock market.

PLoS One 2021 2;16(8):e0255476. Epub 2021 Aug 2.

School of Economics and Management, University of Science and Technology Beijing, Beijing, People's Republic of China.

Financial bubbles have always been a topic of long-term concern for economists. Understanding bubble phenomenon and dating the period of bubbles in real time can provide an early warning diagnosis for financial bubbles and help regulatory authorities to control it and maintain market order. The generalized sup ADF (GSADF) and backward sup ADF (BSADF) tests with flexible window width can effectively detect and date periodically collapsing bubbles in real time. Based on the financial present value model, this paper applies right-tail recursive ADF test to test multiple bubbles in China's multi-level stock market. Unlike the other researches in China, the ratios of the real stock prices' natural logarithm to the real dividends' natural logarithm are used for our testing instead of stock price index. Empirical results show that there are 8 bubbles in the Main-Board Market, 6 bubbles in the Small and Medium Enterprises Board (SMEs), and 4 bubbles in the Growth Enterprise Market (GEM). These bubbles are liquidity-driven and presuppose a loose credit cycle, with the exception of bubbles in 2014-2015. The frequent emergence of bubbles in a short time indicates that China's stock market is still emerging market. In addition, frequent fluctuations imply there is a serious "herd effect" and a lack of monitoring mechanism for bubble risk. This study not only enrich the real-time dynamic research on periodical bubbles of China's stock market, but also provide an empirical reference for investors' investment choices, financial decisions of listed companies and warning mechanism of regulatory authorities.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255476PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328329PMC
August 2021

Low-Temperature Co-hydroxylated Cu/SiO Hybrid Bonding Strategy for a Memory-Centric Chip Architecture.

ACS Appl Mater Interfaces 2021 Aug 28;13(32):38866-38876. Epub 2021 Jul 28.

State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001, China.

Cu/SiO hybrid bonding with planarized dielectric and isolated metal connections can realize ultradense interconnects (e.g., ≤1 μm) by eliminating the microbumps and underfill through the direct bonding of Cu-Cu and SiO-SiO. However, the low-temperature bonding of Cu-Cu (oxide-free surface) and SiO-SiO (hydroxylated surface) is difficult to be compatible in a mechanism. We circumvent this contradiction by constructing a co-hydroxylated functional surface on a Cu/SiO hybrid platform. By combining and optimizing the protocol of Ar/O plasma activation and formic acid solution immersion, an -OH active layer was successfully established on the Cu and SiO surfaces simultaneously, and the increased total surface area provided more adsorption sites for hydroxyl groups. A Cu-Cu interface with sufficient atom diffusion, substantial grain growth, and fewer microvoids was obtained at 200 °C. Notably, the carbon-related interlayer that may degrade the interfacial performance could be effectively inhibited across the optimized SiO-SiO interface even if organic acid was introduced in the protocol. This low-temperature Cu/SiO hybrid bonding via a co-hydroxylated strategy may inspire the development of a memory-centric chip architecture and functional integrated circuits delivering a monolithic-like performance in the future hyperscaling era.
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http://dx.doi.org/10.1021/acsami.1c09796DOI Listing
August 2021

FISH-Based Karyotype Analyses of Four Dracaena Species.

Cytogenet Genome Res 2021 21;161(5):272-277. Epub 2021 Jul 21.

Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of Medical Sciences & Peking Union Medical College, Jinghong, China.

The genus Dracaena is the main source of dragon's blood, which is a plant resin and has been used as traditional medicine since ancient times in different civilizations. However, the chromosome numbers and karyotypes present in this genus remain poorly understood. In this study, fluorescence in situ hybridization (FISH) using oligonucleotide probes for ribosomal DNAs (5S and 45S rDNA) and telomeric repeats (TTTAGGG)3 was applied to analyze 4 related species: Dracaena terniflora Roxb., Dracaena cambodiana Pierre ex Gagnep., Aizong (Dracaena sp.), and Dracaena cochinchinensis (Lour.) S.C. Chen. In all 4 species, both 5S and 45S rDNA showed hybridization signals in the paracentromeric region of a pair of chromosomes; the sizes of the 45S rDNA signals were larger than those of the 5S rDNA. Importantly, the telomeric repeat signals were located in the telomeric regions of almost all chromosomes. The results indicated that the chromosome number of all 4 Dracaena species is 2n = 40, and the lengths of the mitotic metaphase chromosomes range from 0.99 to 2.98 μm. Our results provide useful cytogenetic information, which will be beneficial to future studies in genome structure of the genus Dracaena.
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http://dx.doi.org/10.1159/000516897DOI Listing
September 2021

Involvement of miR-199a-3p/DDR1 in vascular endothelial cell senescence in diabetes.

Eur J Pharmacol 2021 Oct 13;908:174317. Epub 2021 Jul 13.

Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China; Provincial Key Laboratory of Cardiovascular Research, Central South University, Changsha, 410078, China. Electronic address:

Endothelial cell dysfunction is a prominent feature of diabetic cardiovascular complications, and endothelial cell senescence is considered to be an important contributor to endothelial dysfunction. Discoidin domain receptor 1 (DDR1) has been reported to be involved in atherogenesis and cerebral ischemia/reperfusion injury. In this study, we aimed to explore the role of DDR1 in endothelial cell senescence under diabetic conditions and elucidate the underlying mechanisms. A diabetic rat model was established by a single intraperitoneal injection of streptozocin (STZ) (60 mg/kg), which showed an increase in senescence-associated β-galactosidase (SA-β-gal) staining signal of thoracic aortic endothelium, impaired vascular structure and function, accompanied by an up-regulation of DDR1. Next, we verified the role of DDR1 in endothelial senescence and the underlying mechanisms in high glucose-treated human umbilical vein endothelial cells (HUVECs). Consistent with the in vivo findings, high glucose induced endothelial senescence, impaired endothelial function and elevated DDR1 expression, accompanied by the elevation of senescence-related genes p53 and p21 expression, and these effects were reversed by DDR1 siRNA. DDR1 has been documented to be a potential target of miR-199a-3p. Here, we found that miR-199a-3p was down-regulated by high glucose in the aorta tissue and HUVECs, while miR-199a-3p mimic significantly suppressed increased endothelial senescence and elevated DDR1 induced by high glucose. In conclusion, our data demonstrated that miR-199a-3p/DDR1/p53/p21 signaling pathway was involved in endothelial senescence under diabetic conditions, and therapeutic targeting DDR1 would be exploited to inhibit endothelial senescence owing to high glucose exposure.
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http://dx.doi.org/10.1016/j.ejphar.2021.174317DOI Listing
October 2021

The complete chloroplast genome of (Zingiberaceae: Zingiberea) from Yunnan Province, China.

Mitochondrial DNA B Resour 2021 30;6(8):2114-2116. Epub 2021 Jun 30.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

is a medicinal plant endemic to Yunnan Province, China. In this study, its complete chloroplast genome was assembled and characterized. The total genome size of was 158,484 bp in length, consisting of a large single-copy region (LSC), a small single-copy region (SSC) and two inverted repeat regions (IRs) with 88,605 bp, 15,285 bp and 29,822 bp, respectively. Its GC content was 36.08%. The chloroplast genome encoded 113 unique genes, including 79 protein-coding, 30 tRNA, and four rRNA genes. The result of the phylogenetic analysis indicated that was related to var. and supported de Boer's classification that , , , , var. and belonged to the Clade.
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http://dx.doi.org/10.1080/23802359.2021.1938722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253217PMC
June 2021

Structural Characterization and Assessment of Anti-Inflammatory and Anti-Tyrosinase Activities of Polyphenols from .

Molecules 2021 Jun 26;26(13). Epub 2021 Jun 26.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.

Polyphenols, widely distributed in the genus plants, possess extensive cellular protective effects such as anti-inflammatory, anti-tyrosinase, and anti-obesity, which makes it a potential anti-inflammatory drug or enzyme inhibitor. Therefore, the aim of this study is to screen for the anti-inflammatory and enzyme inhibitory activities of compounds from title plant. Using silica gel, MCI, ODS C18, and Sephadex LH-20 column chromatography, as well as semipreparative HPLC, the extract of roots was separated. Four new ellagitannins, Whiskey tannin C (), 1--(4-methoxygalloyl)-6--galloyl-2,3--()-hexahydroxydiphenoyl--d-glucose (), 1--galloyl-6--(3-methoxygalloyl)-2,3--()-hexahydroxydiphenoyl--d-glucose (), and 1--galloyl-6--vanilloyl-2,3--()-hexahydroxydiphenoyl--d-glucose (), along with eight known polyphenols were firstly obtained from this plant. The structures of all isolates were elucidated by HRMS, NMR, and CD analyses. Using lipopolysaccharide (LPS)-stimulated RAW2 64.7 cells, we investigated the anti-inflammatory activities of compounds -, unfortunately, none of them exhibit inhibit nitric oxide (NO) production, their IC values are all > 50 μM. Anti-tyrosinase activity assays was done by tyrosinase inhibition activity screening model. Compound showed weak tyrosinase inhibitory activity with IC values of 426.02 ± 11.31 μM. Compounds - displayed moderate tyrosinase inhibitory activities with IC values in the range of 124.74 ± 3.12-241.41 ± 6.23 μM. The structure-activity relationships indicate that hydroxylation at C-3', C-4', and C-3 in the flavones were key to their anti-tyrosinase activities. The successful isolation and structure identification of ellagitannin provide materials for the screening of anti-inflammatory drugs and enzyme inhibitors, and also contribute to the development and utilization of .
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http://dx.doi.org/10.3390/molecules26133913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271706PMC
June 2021

Direct observation of chaotic resonances in optical microcavities.

Light Sci Appl 2021 Jun 30;10(1):135. Epub 2021 Jun 30.

Ministry of Industry and Information Technology Key Lab of Micro-Nano Optoelectronic Information System, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, China.

Optical microcavities play a significant role in the study of classical and quantum chaos. To date, most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outputs, without directly interrogating the dynamics and the associated mode patterns inside. As a result, this key information is rarely retrieved with certainty, which significantly restricts the verification and understanding of the actual chaotic motion. Here we demonstrate a simple and robust approach to directly and rapidly map the internal mode patterns in chaotic microcavities. By introducing a local index perturbation through a pump laser, we report a spectral response of optical microcavities that is proportional to the internal field distribution. With this technique, chaotic modes with staggered mode spacings can be distinguished. Consequently, a complete chaos assisted tunneling (CAT) and its time-reversed process are experimentally verified in the optical domain with unprecedented certainty.
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http://dx.doi.org/10.1038/s41377-021-00578-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241958PMC
June 2021

Characterization of a novel shrimp pathogen, Vibrio brasiliensis, isolated from Pacific white shrimp, Penaeus vannamei.

J Fish Dis 2021 Oct 21;44(10):1543-1552. Epub 2021 Jun 21.

Key Laboratory of Marine Aquaculture Disease Control, Ministry of Agriculture, Key Laboratory of Marine Aquaculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.

A novel pathogenic strain Vibrio 20190611023 was isolated from the hepatopancreas of moribund cultured Penaeus vannamei suffering from black gill disease. This strain was identified as V. brasiliensis based on the phylogenetic analyses of 16S rDNA gene and five other housekeeping genes (i.e., gapA, ftsZ, mreB, topA and gyrB). Some biochemical features of this strain were determined with an API 20NE system, and its haemolytic activity was determined using a sheep blood agar plate. The pathogenicity of this isolate 20190611023 was confirmed by the experimental challenge tests and histopathological examinations. P. vannamei were challenged via reverse gavage with different doses of bacterial suspensions. The calculated median lethal dose (LD ) was (3.16 ± 1.78) × 10  CFU/g (body weight). Moreover, antibiotic susceptibility tests were performed, the results of which showed that the strain 20190611023 was sensitive to chloramphenicol, compound sulphamethoxazole, ciprofloxacin, doxycycline and oxacillin, but resistant to erythromycin, kanamycin, gentamicin, cefoperazone, ceftriaxone, cefamezin and piperacillin. To our knowledge, this is the first report for demonstrating V. brasiliensis as a shrimp pathogen, which expands the host range of V. brasiliensis infection. The present study highlights that more attention should be paid to this novel pathogen in intensive shrimp aquaculture.
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http://dx.doi.org/10.1111/jfd.13475DOI Listing
October 2021

Magnetic Molecularly Imprinted Polymers Based on Dehydroabietylamine as Chiral Monomers for the Enantioseparation of -Mandelic Acid.

ACS Omega 2021 Jun 3;6(23):14977-14984. Epub 2021 Jun 3.

Department of Pharmaceutical Engineering, Medical College, Guangxi University, Nanning 530004, China.

Stereoselective adsorption of the enantiomers shows potential in the resolution of a racemate. In this work, we synthesized novel magnetic surface molecularly imprinted polymers (MIPs) on the surface of the γ-methacryloxypropyltrimethoxysilane (MPS)-modified [email protected] particles to utilize chiral dehydroabietylamine (DHA) as a functional monomer and -mandelic acid as a template molecule (DHA-MIPs). We performed the resolution of mandelic acid racemate (-MA) via adsorption on the as-prepared MIPs. The results revealed that the MIPs have good affinity and high adsorption capacity for -MA and show better enantioselective adsorption ability for -MA than that for -MA. One-stage adsorption of -MA on the MIPs can achieve up to 53.7% enantiomeric excess (ee) for -MA. These help us to improve the chiral separation ability of the traditional MIPs using a chiral rather than an achiral monomer in MIP preparation. The MIPs can be employed as an economic and efficient adsorbent for chiral separation of MA racemate.
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http://dx.doi.org/10.1021/acsomega.1c01054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209806PMC
June 2021

Effect of Hyperbaric Oxygen Therapy on Polarization Phenotype of Rat Microglia After Traumatic Brain Injury.

Front Neurol 2021 3;12:640816. Epub 2021 Jun 3.

Department of Hyperbaric Oxygen, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

The neurological defect caused by secondary damage following traumatic brain injury (TBI) is considered critical for the management of TBI. Microglia (MG) are a resident brain macrophage that could differentiate into M1 type or M2 type in response to injury and repair. It is known that the MG transition from M1 phenotype to anti-inflammatory M2 phenotype might reduce secondary injury of TBI. So, a TBI animal model was established and we compared biomarkers of M1 and M2MG between the controls and experimental animals receiving hyperbaric oxygen therapy (HBOT). This study aimed to explore whether HBOT was an effective method to improve neural functional recovery promoting the polarization of MG into M2 after TBI. The rats were randomly divided into four groups: SH (Sham-operated), SH + HBO (hyperbaric oxygen), TBI, and TBI + HBO. Each group included 42 rats, and each of these were divided into the following groups: 1, 6, 12, 24, 72 h, 7, and 14 days. The expression of M1 biomarker inducible nitric oxide synthase (iNOS), M2 biomarker arginase 1 (Arg1), associated cytokine tumor necrosis factor-α (TNF-α), and transforming growth factor-β1 (TGF-β1) was evaluated after the observation time. TBI significantly increased the expression levels of M1 marker iNOS and M2 markers Arg1 at different time points. The increased expression of iNOS was suppressed, while the expression level of Arg1 was enhanced by HBOT. Moreover, HBOT suppressed the pro-inflammatory TNF-α secreted by M1, and promoting the anti-inflammatory TGF-1β. In the present study, HBOT showed the effects on shift of M1 toward M2 phenotype with increased expression of M2 biomarkers and decreased expression of M1 biomarkers in the early stage after TBI.
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http://dx.doi.org/10.3389/fneur.2021.640816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209338PMC
June 2021

Ubiquitination and degradation of NF90 by Tim-3 inhibits antiviral innate immunity.

Elife 2021 06 10;10. Epub 2021 Jun 10.

Beijing Institute of Basic Medical Sciences, Beijing, China.

Nuclear factor 90 (NF90) is a novel virus sensor that serves to initiate antiviral innate immunity by triggering stress granule (SG) formation. However, the regulation of the NF90-SG pathway remains largely unclear. We found that Tim-3, an immune checkpoint inhibitor, promotes the ubiquitination and degradation of NF90 and inhibits NF90-SG-mediated antiviral immunity. Vesicular stomatitis virus (VSV) infection induces the up-regulation and activation of Tim-3 in macrophages, which in turn recruit the E3 ubiquitin ligase TRIM47 to the zinc finger domain of NF90 and initiate a proteasome-dependent degradation via K48-linked ubiquitination at Lys297. Targeted inactivation of Tim-3 enhances the NF90 downstream SG formation by selectively increasing the phosphorylation of protein kinase R and eukaryotic translation initiation factor 2α, the expression of SG markers G3BP1 and TIA-1, and protecting mice from VSV challenge. These findings provide insights into the crosstalk between Tim-3 and other receptors in antiviral innate immunity and its related clinical significance.
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http://dx.doi.org/10.7554/eLife.66501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225388PMC
June 2021

Construction of a Cascade Catalyst of Nanocoupled Living Red Blood Cells for Implantable Biofuel Cell.

ACS Appl Mater Interfaces 2021 Jun 8;13(24):28010-28016. Epub 2021 Jun 8.

Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering and College of Physics and Materials Science, Henan Normal University, Xinxiang, Henan 453007, P. R. China.

The broad applications of implantable glucose biofuel cells (GBFCs) have become very attractive in biomedical sciences. The key challenge of GBFCs is eliminating the inevitable product HO generated from the oxidation of glucose when glucose oxidase (GOx) is used as a catalyst while improving the performance of GBFCs. In this work, the cascade electrocatalyst, [email protected] was obtained through the in situ polymerization of dopamine to form nanopolydopamine (NPDA) on the surface of red blood cells (RBCs). The [email protected] can catalyze both fuels of HO and O, so as to generate a high cathodic current (0.414 mA cm). Furthermore, when [email protected] was used as a cathodic catalyst in the membraneless GBFC, it exhibited the cascade catalytic activity in the reduction of O-HO and minimized the damage to RBCs caused by the high concentration of HO. The mechanism research indicates that [email protected] integrates the property of NPDA and RBCs. Specifically, NPDA plays a catalase-like role in catalyzing the decomposition of HO, while RBCs play a laccase-like role in electrocatalyzing the O reduction reaction. This work offers the cascade catalyst for improving the performance of implantable GBFC and presents a strategy for constructing catalysts using living cells and nanomaterials to replace deformable and unstable enzymes in other biofuel cells.
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http://dx.doi.org/10.1021/acsami.1c01479DOI Listing
June 2021

Digital Twin-Enabled Online Battlefield Learning with Random Finite Sets.

Comput Intell Neurosci 2021 13;2021:5582241. Epub 2021 May 13.

College of Systems Engineering, National University of Defense Technology, Changsha 410073, China.

The digital twin is becoming the most promising emerging technology in the field of unmanned combat and has the potential to innovate future combat styles. Online battlefield learning is one of the key technologies for supporting the successful application of digital twin in unmanned combat. Since there is an urgent need for effective algorithms for online learning the battlefield states in real time, a new random finite set- (RFS-) based algorithm is proposed in the presence of detection uncertainty including clutters, missed detection, and noises. The system architecture and operational mode for implementing the digital twin-enabled online battlefield learning are provided. The unmanned ground vehicle (UGV) is employed as the experimental subject for systematically describing the proposed algorithm. The system architecture for implementing the digital twin-enabled online battlefield learning is firstly given, and its operational mode is also described in detail. The RFS-based digital twin models including the battlefield state model, UGV motion model, and sensor model are designed. The Bayesian inference is adopted, and the probability hypothesis density (PHD) filter is modified to implement the online learning process. At last, a group of experiments are conducted to verify the performance and effectiveness of the proposed algorithm. The research work in this paper will provide a good demonstration of the application of digital twin in unmanned combat.
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http://dx.doi.org/10.1155/2021/5582241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137298PMC
July 2021
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