Publications by authors named "Gang Zhao"

1,524 Publications

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Highly Stable and Selective Sensing of Hydrogen Sulfide in Living Mouse Brain with NiN Single-Atom Catalyst-Based Galvanic Redox Potentiometry.

J Am Chem Soc 2022 Aug 4. Epub 2022 Aug 4.

College of Chemistry, Beijing Normal University, Beijing 100875, China.

Hydrogen sulfide (HS) is recognized as a gasotransmitter and multifunctional signaling molecule in the central nervous system. Despite its essential neurofunctions, the chemical dynamics of HS during physiological and pathological processes remains poorly understood, emphasizing the significance of HS sensor development. However, the broadly utilized electrochemical HS sensors suffer from low stability and sensitivity loss in vivo due to sulfur poisoning-caused electrode passivation. Herein, we report a high-performance HS sensor that combines single-atom catalyst strategy and galvanic redox potentiometry to overcome the issue. Atomically dispersed NiN active sites on the sensing interface promote electrochemical HS oxidation at an extremely low potential to drive spontaneous bipolarization of a single carbon fiber. Bias-free potentiometric sensing at open-circuit condition minimizes sulfur accumulation on the electrode surface, thus significantly enhancing the stability and sensitivity. The resulting sensor displays high selectivity to HS against physiological interferents and enables real-time accurate quantification of HS-releasing behavior in the living mouse brain.
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http://dx.doi.org/10.1021/jacs.2c04695DOI Listing
August 2022

Epidemiology, Characteristic, and Prognostic Factors of Primary Sporadic Intradural Malignant Peripheral Nerve Sheath Tumor in the Spinal Canal: A Systematic Literature Review.

Front Oncol 2022 8;12:911043. Epub 2022 Jul 8.

Department of Neurosurgery, the First Hospital of Jilin University, Changchun, China.

Purpose: Primary sporadic intradural malignant peripheral nerve sheath tumor (MPNST) in the spinal canal is a type of rare neoplasm with challenging diagnosis and therapy. The overall prognosis of this tumor is markedly different from that of the usual spinal intradural tumors. The purpose of this systematic review is to reduce the misdiagnosis and enhance the prognosis of the disease by reviewing the literature.

Methods: PubMed, Medline, and Embase databases were searched for articles in English language published from 1980 to May 2021, yielding 500 potentially relevant articles. The keywords were as follows: "spinal", "malignant peripheral nerve sheath tumor", "neurosarcoma", "malignant schwannoma", and "malignant neurofibroma". Thirteen papers met the eligibility criteria, including 55 cases with spinal intradural primary sporadic MPNSTs, which were confirmed by post-operation pathology. We further analyzed the clinical manifestations, radiological manifestations, pathological features, comprehensive treatment strategies, and prognosis.

Results: Fifty-five spinal intradural primary sporadic MPNSTs from 30 (54.5%) male and 25 (45.5%) female patients with an average age at diagnosis of 40 years (range, 3-70 years) were included in the study. The most common clinical manifestations were local or radicular pain and motor disturbance. All tumors had significant enhancement and heterogeneous enhancement was more common. Out of 18 lesions, 14 were diagnosed as high grade and the remaining 4 were diagnosed as low grade. The ki-67 labeling index ranged from 5% to 60%. The median recurrence and survival time were 36 and 72 months, respectively. The log-rank tests indicated that significant predictors of OS were patient age (≤30 vs. >30 years) at the time of diagnosis and the presence of metastatic disease, and similar analyses for RFS demonstrated that the presence of metastatic disease was the only significant predictor (60 vs. 10 months). The multivariate Cox proportional hazards regression analysis revealed that absence of metastasis was an independent factor for predicting a favorable prognosis.

Conclusions: Spinal intradural primary sporadic MPNSTs are challenging malignant tumors without a systematic treatment plan. The factors affecting its prognosis are not clear. Even after surgical treatment and adjuvant treatment, the recurrence rate and mortality rate are still high. Clinicians should be alert to the possibility of this disease and achieve early detection and treatment.
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http://dx.doi.org/10.3389/fonc.2022.911043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309257PMC
July 2022

Mechanical Gradients Enable Highly Stretchable Electronics Based on Nanofiber Substrates.

ACS Appl Mater Interfaces 2022 Aug 27;14(31):35997-36006. Epub 2022 Jul 27.

Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei 230027, China.

Stretchable electronics play a pivotal role in the age of information and intelligence. Integrated circuit components are an integral part of high-performance and multifunctional stretchable electronic devices. Therefore, it is an ideal design concept for stretchable electronic devices to not only ensure the reliability of the connection between rigid inorganic electronic components and stretchable circuits but also maintain the stretchability of the device. In this work, we constructed a mechanical gradient strategy to fabricate high-performance stretchable electronic devices. Briefly, polyvinyl alcohol glue is used to fix integrated circuits on stretchable circuits, which are fabricated by printing liquid metal on a thermoplastic polyurethane nanofiber membrane. The strategy of integrated circuits (rigid)-polyvinyl alcohol glue (high elastic modulus)-thermoplastic polyurethane nanofiber membrane (low elastic modulus)-liquid metal (liquid) realizes the strain gradient during the stretching process of the device, thus ensuring the stability and reliability. Moreover, we explored the mechanism through experiments and finite element analysis. The flexible electronic devices fabricated by this scheme are not only ultra-stretchable (900%) but also have good stability and comfort. As proof, the application in stretchable sensors, human-computer interaction devices, and displays was realized.
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http://dx.doi.org/10.1021/acsami.2c10245DOI Listing
August 2022

Portevin-Le Chatelier Characterization of Quenched Al-Mg Alloy Sheet with Different Mg Concentrations.

Materials (Basel) 2022 Jul 17;15(14). Epub 2022 Jul 17.

School of Materials Science & Engineering, Northeastern University, Wenhua Road, No. 3-11, Heping District, Shenyang 110819, China.

In the present study, the PLC characteristic parameters and DSA mechanism of Al-(2.86~9.41) Mg alloy sheets were investigated during tensile testing at room temperature with a tensile rate of 1 × 10 s. On the basis of the solution Mg concentrations in the α-Al matrix, the initial vacancy concentration, the second-phase particle configuration and the recrystallized grain configuration are almost the same by quenching treatment. The results show that the type of room-temperature tensile stress-strain curves of quenched Al-(2.86~9.41) Mg alloy sheets varied according to the Mg content. The type of stress-strain curve of the Al-2.86 Mg alloy sheet was B + C, while the type of stress-strain curve of the Al-(4.23~9.41) Mg alloy sheets was C. When the quenched Al-(2.86~9.41) Mg alloy sheets were stretched at room temperature, the strain cycle of the rectangular waves corresponding to the high stress flow ΔεTmax and stress drop amplitude Δσ on the zigzag stress-strain curve of alloy sheets increased with increasing the Mg content. Moreover, the strain cycle of ΔεTmax and Δσ on the stress-strain curve of alloy sheets increased gradually with increasing tensile deformation. The yield stress of quenched Al-(2.86~9.41) Mg alloy sheets increased gradually with increasing the Mg content. Moreover, the critical strain corresponding to yield stress εσ and the critical strain corresponding to the occurrence of the PLC shearing band εc of alloy sheets both increased with increasing the Mg content. However, the difference in flow strain value Δεc-σ between εc and εσ of alloy sheets decreased gradually with increasing the Mg content.
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http://dx.doi.org/10.3390/ma15144965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322177PMC
July 2022

The optimal tension for the reconstruction of the distal radioulnar ligaments.

Int Orthop 2022 Jul 26. Epub 2022 Jul 26.

Department of Sports Medicine, Wuxi 9Th People's Hospital Affiliated to Soochow University, Wuxi, Jiangsu, China.

Purpose: This study aimed to investigate the optimal tension for the reconstruction of the distal radioulnar ligaments (DRULs) in the treatment of the distal radioulnar joint (DRUJ) instability.

Methods: A total of eight human cadaver upper extremities were used. First, the Tekscan sensor film system was used to measure the contact characteristics of the intact DRUJ. Following this, the DRULs were resected, and the measurement was repeated. The DRULs were then reconstructed according to Adams' procedure, and the contact forces under different initial tension were compared with that of the intact group to obtain the optimal tension. At that point, the contact force of the DRUJ was close to normal. The reliability of the obtained tension was verified by translational testing, which reflected the stability of the DRUJ.

Results: In the neutral position, the contact force, area, and pressure inside DRUJ were 0.51 ± 0.10 N, 64.08 ± 11.58 mm, and 8.33 ± 2.42 kPa, respectively. After the DRULs were resected, they were 0.19 ± 0.02 N, 41.75 ± 5.01 mm, and 4.86 ± 1.06 kPa, respectively. The relationship between the tension and contact force was linear regression (Y = 0.0496x + 0.229, R = 0.9575, P < 0.0001). According to the equation, when the tension was 3.64-7.68 N, the contact force was close to normal. There was no statistical difference in the stability of the reconstructed DRUJ under this tension compared with the intact group (P = 0.08).

Conclusion: By comparing the contact forces under different reconstruction tensions with the normal value, we obtained the optimal tension, which can provide the theoretical basis for the clinical treatment of chronic DRUJ instability.
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http://dx.doi.org/10.1007/s00264-022-05525-8DOI Listing
July 2022

Occurrence and exposure risk assessment of pesticide residues in green tea samples cultivated in Hangzhou area, China.

Food Addit Contam Part B Surveill 2022 Jul 25:1-6. Epub 2022 Jul 25.

Laboratory of Chemistry and Physics, Hangzhou Center for Disease Control and Prevention, Hangzhou, P. R. China.

The concentration of pesticide residues in 105 green tea samples grown in Hangzhou area were investigated. Of the 14 pesticides analysed using gas chromatography-tandem mass spectrometry and the 27 pesticides analysed using liquid chromatography-tandem mass spectrometry, only 18 were detected in the tea samples. The most frequently detected pesticide residues were imidacloprid (35.2%), acetamiprid (26.7%), carbendazim (21.0%), bifenthrin (21.0%), and cyhalothrin (19.1%). Carbofuran was the only pesticide which exceeded in one sample the maximum residue limit. The concentrations of the analytes in tea samples ranged from below the limit of detection (LOD) to 2.64 mg/kg. Their mean concentrations were all below the LOD, except for imidacloprid, acetamiprid, carbendazim, bifenthrin and cyhalothrin. Based on a preliminary long-term exposure assessment, the hazard quotient values of the detected pesticides varied in the range 0.47 × 10 - 1.1 × 10%, which indicates that these levels did not pose a risk to human health in Hangzhou area.
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http://dx.doi.org/10.1080/19393210.2022.2103746DOI Listing
July 2022

[Correlation analysis of Poor Prognosis and Immunotherapy of lncRNAs Related with m A Modification in Cervical Cancer].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 Jul;53(4):626-636

Lab of Experimental Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To study the correlation between N -methyladenosine (m A)-modification-associated long non-coding RNAs (lncRNAs) and poor prognosis and immunotherapy in cervical cancer based on data mining of The Cancer Genome Atlas (TCGA) cervical cancer dataset, so as to assess effectively the prognosis of cervical cancer patients and the feasibility of immunotherapy.

Methods: We identified m A-modification-associated lncRNAs correlated to the prognosis of cervical cancer by conducting bioinformatics analysis of cervical cancer samples from the TCGA datasets and constructed a prognostic risk model of cervical cancer accordingly.

Results: A total of 343 m A-modification-associated lncRNAs were identified from the samples of 304 cervical cancer patients. Univariate Cox regression analysis showed that 26 out of the 343 m A-modification-associated lncRNAs were significantly associated with the prognosis of cervical cancer patients. We identified 7 m A-modification-associated lncRNAs, including DLEU1, AC099850.4, DDN-AS1, EP300-AS1, AC131159.1, AL441992.2, and AL021707.6 through Lasso regression analysis and then developed a prognostic risk model based on them. According to the Kaplan-Meier survival analysis, cervical cancer patients in the low-risk group exhibited significantly improved overall survival (OS) in comparison with those in the high-risk group ( <0.001). The area under the curve ( ) of receiver operating characteristic (ROC) curve analysis demonstrated the high sensitivity and credibility of the risk model. Multivariate Cox analysis showed that the risk score was an independent prognostic factor of cervical cancer patients. Tumor immune dysfunction and exclusion (TIDE) analysis predicted that the high-risk group would benefit more from immunotherapy. In addition, we found that immune checkpoint PD1 was associated with the expression of m6A-modification-related lncRNAs such as DDN-AS1, and the expression was higher in the high-risk group ( <0.05).

Conclusion: The prognostic risk model constructed on the basis of the aforementioned 7 m A-modification-associated lncRNAs can be used to effectively predict the prognosis of cervical cancer patients and assess the efficacy of immunotherapy targeting PD1.
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http://dx.doi.org/10.12182/20220760504DOI Listing
July 2022

Landscape of somatic alterations in large-scale solid tumors from an Asian population.

Nat Commun 2022 Jul 23;13(1):4264. Epub 2022 Jul 23.

Department of Gastrointestinal Oncology, Huifuxi Branch of Guangdong Provincial People's Hospital, Guangzhou, China.

Extending the benefits of tumor molecular profiling for all cancer patients requires a comprehensive analysis of tumor genomes across distinct patient populations worldwide. In this study, we perform deep next-generation DNA sequencing (NGS) from tumor tissues and matched blood specimens from over 10,000 patients in China by using a 450-gene comprehensive assay, developed and implemented under international clinical regulations. We perform a comprehensive comparison of somatically altered genes, the distribution of tumor mutational burden (TMB), gene fusion patterns, and the spectrum of various somatic alterations between Chinese and American patient populations. Here, we show 64% of cancers from Chinese patients in this study have clinically actionable genomic alterations, which may affect clinical decisions related to targeted therapy or immunotherapy. These findings describe the similarities and differences between tumors from Chinese and American patients, providing valuable information for personalized medicine.
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http://dx.doi.org/10.1038/s41467-022-31780-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308789PMC
July 2022

Double Stent Retriever Mechanical Embolectomy of a Refractory Onyx Embolus in the Basilar Artery.

J Vasc Interv Radiol 2022 Jul 13. Epub 2022 Jul 13.

Department of Neurosurgery, General Hospital of Southern Theatre Command, Guangzhou, China; Address: 111 Liuhua Road, Yuexiu District, Guangzhou, China. 510010.

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http://dx.doi.org/10.1016/j.jvir.2022.07.005DOI Listing
July 2022

One-step laparoscopic pancreatic necrosectomy verse surgical step-up approach for infected pancreatic necrosis: a case-control study.

World J Emerg Med 2022 ;13(4):274-282

Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Background: The surgical step-up approach often requires multiple debridements and might not be suitable for infected pancreatic necrosis (IPN) patients with various abscesses or no safe route for percutaneous catheter drainage (PCD). This case-control study aimed to investigate the safety and effectiveness of one-step laparoscopic pancreatic necrosectomy (LPN) in treating IPN.

Methods: This case-control study included IPN patients undergoing one-step LPN or surgical step-up in our center from January 2015 to December 2020. The short-term and long-term complications after surgery, length of hospital stay, and postoperative ICU stays in both groups were analyzed. Univariate and multivariate logistic regression analyses were performed to explore the risk factors of major complications or death.

Results: A total of 53 IPN patients underwent one-step LPN and 37 IPN patients underwent surgical step-up approach in this study. There was no significant difference in the incidence of death, major complications, new-onset diabetes, or new-onset pancreatic exocrine insufficiency between the two groups. However, the length of hospital stay in the one-step LPN group was significantly shorter than that in the surgical step-up group. Univariate regression analysis showed that the surgical approach (one-step/step-up) was not the risk factor for major complications or death. Multivariate logistic regression analysis indicated that computed tomography (CT) severity index, American Society of Anesthesiologists (ASA) class IV, and white blood cell (WBC) were the significant risk factors for major complications or death.

Conclusion: One-step LPN is as safe and effective as the surgical step-up approach for treating IPN patients, and reduces total hospital stay.
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http://dx.doi.org/10.5847/wjem.j.1920-8642.2022.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233970PMC
January 2022

College Smart Classroom Attendance Management System Based on Internet of Things.

Comput Intell Neurosci 2022 5;2022:4953721. Epub 2022 Jul 5.

Architectural Engineering School, Qingdao Huanghai University, Qingdao, Shandong 266427, China.

Since entering the information age, educational informatization reform has become the inevitable trend of the development of colleges and universities. The traditional education management methods, especially the classroom attendance methods, not only need to rely on a large number of manpower for data collection and analysis but also cannot dynamically monitor students' attendance and low efficiency. The development of Internet of things technology provides technical support for the informatization reform of education management in colleges and universities and makes the classroom attendance management in colleges and universities have a new development direction. In this study, a college smart classroom attendance management system based on RFID technology and face recognition technology is constructed under the architecture of the Internet of things, and the corresponding simulation experiments are carried out. The experimental results show that the smart classroom attendance management system based on RFID technology can accurately identify the absence and substitution of students and has the advantages of fast response and low cost. However, its recognition is easily affected by obstructions, which requires students to place identification cards uniformly. The smart classroom attendance management system based on face recognition technology can accurately record and identify the situation of students entering and leaving the classroom and identify the situations of being late and leaving early, absenteeism, and substitute classes. The experimental results are basically consistent with the sample results, and the error rate is low. However, the system is easily affected by environmental light, students' sitting posture, expression, and other factors, so it cannot be recognized. Generally speaking, both can meet the needs of classroom attendance in colleges and universities and have high accuracy and efficiency.
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http://dx.doi.org/10.1155/2022/4953721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276505PMC
July 2022

Prognostic role of myeloid-derived tumor-associated macrophages at the tumor invasive margin in gastric cancer with liver metastasis (GCLM): a single-center retrospective study.

J Gastrointest Oncol 2022 Jun;13(3):1340-1350

Department of Gastrointestinal Surgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Liver metastasis is one of the important factors leading to poor prognosis of gastric cancer. According to the classic "seed soil theory", it is speculated that the liver microenvironment at the invasion margin of gastric cancer liver metastases (GCLM) may have a crucial impact on tumor progression. However, few studies had stated the correlation between the patients' prognosis and the densities of stromal cells infiltrating into the invasive margin, where our retrospective study designed to identify the role of infiltrating macrophages on the prognosis of GCLM as a reliable supplement of predictive tumor markers.

Methods: The material consisted of a group of 72 gastric cancer (GC) patients with liver metastasis diagnosed from February 2015 and December 2020. The CD68, CD206, and Clec4f macrophages in their specimens were counted by immunohistochemistry (IHC), and the analysis area was the invasive margin of metastatic lesions. Clinical data were collected retrospectively. Overall survival (OS) was calculated from the date of initial diagnosis to the date of last follow-up or death. Survival analyses were performed using the Kaplan-Meier method and log-rank test. Multivariate Cox regression was performed to asses impact of macrophages on OS.

Results: The expression of CD206 could indicate the prognosis of patients with GCLM, and patients with high expression of CD206 had worse prognoses (P=0.0002). Univariate and multivariate analyses showed that CD206 was an independent risk factor for prognosis (HR 5.276, 95% CI: 1.730-16.089, P=0.003).

Conclusions: The CD206 myeloid-derived tumor associated macrophages (TAMs) may predict whether patients could benefit from R1 resection of liver-metastatic lesions, which has important theoretical significance and practical value for accurately evaluating the clinical prognosis of patients with GCLM and guiding clinical treatment.
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http://dx.doi.org/10.21037/jgo-22-530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274044PMC
June 2022

Efficacy and Safety of Camrelizumab in Combination with Docetaxel + S-1 Sequenced by Camrelizumab + S-1 for Stage III (PD-1+/MSI-H/EBV+/dMMR) Gastric Cancer: Study Protocol for a Single-Center, Prospective, Open-Label, Single-Arm Trial.

Front Surg 2022 28;9:917352. Epub 2022 Jun 28.

Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. A higher incidence of gastric cancer had been found in East Asia compared to the other regions. Gastrectomy with radical lymph node dissection is the cornerstone of curative treatment for Stage III gastric cancer, and postoperative systemic chemotherapy with docetaxel, S-1 improved patients' disease-free survival rates. However, advances in immunotherapy bring innovations in the management of patients with gastric cancer. The objective of this study was to explore the efficacy and safety of camrelizumab in combination with docetaxel + S-1, sequenced by camrelizumab + S-1 in stage III gastric cancer patients who are EBV positive, with defective mismatch repair and CPS ≥5.

Methods And Analysis: This prospective, open-label, single-arm trial was performed at Renji Hospital. In this study, a total of 70 adult patients aged 18-80 years with Stage III (PD-1+/MSI-H/EBV+/dMMR) gastric cancer confirmed by post-operative pathology will be enrolled after screening. Participants will receive the specific chemotherapy regimen until 1 year after the operation or until tumor recurrence or metastasis. The primary outcome is the 3-year disease-free survival rate measured by the Clopper-Pearson method and 95% confidence intervals. The secondary outcomes include overall survival, incidence and severity of adverse effects, and laboratory abnormalities. The data will be analyzed by the Kaplan-Meier method and log-rank test. The patients will be followed up every 3 months with imaging investigation until clinical remission.

Ethics And Dissemination: All participants will provide informed consent. The protocol has been approved by the Shanghai Jiaotong University School of Medicine, Renji Hospital Ethics Committee (KY2019-191). The results will be disseminated through peer-reviewed manuscripts, reports and presentations.

Clinical Trial Registration: ClinicalTrials.gov, identifier: ChiCTR1900027123. Registration date November 2019; first enrolment December 2019; expected end date December 2021; trial status: Ongoing.

Brief Abstract: A clinical trial for Stage III (PD-1+/MSI-H/EBV+/dMMR) gastric cancer patients who accepted anti-PD-1 therapy combined with docetaxel + S-1 as the first-line treatment and explored improvements in three-year disease-free survival rate.
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http://dx.doi.org/10.3389/fsurg.2022.917352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274117PMC
June 2022

Effect of Nb Addition and Heat Input on Heat-Affected Zone Softening in High-Strength Low-Alloy Steel.

Materials (Basel) 2022 Jun 26;15(13). Epub 2022 Jun 26.

School of Metallurgical Engineering, Anhui University of Technology, Maanshan 243032, China.

The effect of both Nb content and heat input on the softening phenomenon of the heat-affected zone (HAZ) of low-alloy high-strength steel was studied through welding thermal simulation experiments. The microstructure evolution, density variation of geometrically necessary dislocation, microhardness distribution and the second phase precipitation behavior in HAZ was characterized and analyzed by combining the optical microscope, scanning electron microscope, high-resolution transmission electron microscope with microhardness tests. The results showed that the softening appeared in the fine-grain HAZ (FGHAZ) of the low-alloy high-strength steel with the polygonal ferrite and bainite microstructure. With an increase in Nb content, the FGHAZ softening was inhibited even with high heat input; however, the hardness shows little variation. On the one hand, the increase in the Nb content increased the volume fraction of high-strength bainite in the FGHAZ. On the other hand, the remarkable strengthening was produced by the equally distributed precipitation nanoparticles. As a result, the two factors were the main reason for the solution of the FGHAZ softening problem in the low-alloyed high-strength steel with the mixed microstructure of ferrite and bainite.
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http://dx.doi.org/10.3390/ma15134503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267242PMC
June 2022

An Immune Signature for Risk Stratification and Therapeutic Prediction in Helicobacter pylori-Infected Gastric Cancer.

Cancers (Basel) 2022 Jul 4;14(13). Epub 2022 Jul 4.

Department of Gastrointestinal Surgery, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.

Helicobacter pylori (HP) infection is the greatest risk factor for gastric cancer (GC). Increasing evidence has clarified that tumor immune microenvironment (TIME) is closely related to the prognosis and therapeutic efficacy of HP-positive (HP+) GC patients. In this study, we aimed to construct a novel immune-related signature for predicting the prognosis and immunotherapy efficacy of HP+ GC patients. A total of 153 HP+ GC from three different cohorts were included in this study. An Immune-Related prognostic Signature for HP+ GC patients (IRSHG) was established using Univariate Cox regression, the LASSO algorithm, and Multivariate Cox regression. Univariate and Multivariate analyses proved IRSHG was an independent prognostic predictor for HP+ GC patients, and an IRSHG-integrated nomogram was established to quantitatively assessthe prognostic risk. The low-IRSHG group exhibited higher copy number load and distinct mutation profiles compared with the high-IRSHG group. In addition, the difference of hallmark pathways and immune cells infiltration between the two groups was investigated. Notably, tumor immune dysfunction and exclusion (TIDE) analysis indicated that the low-IRSHG group had a higher sensitivity to anti-PD-1 immunotherapy, which was validated by an external pabolizumab treatment cohort. Moreover, 98 chemotherapeutic drugs and corresponding potential biomarkers were identified for two groups, and several drugs with potential ability to reverse IRSHG score were identified using CMap analysis. Collectively, IRSHG may serve as a promising biomarker for survival outcome as well as immunotherapy efficacy. Furthermore, it can also help to prioritize potential therapeutics for HP+ GC patients, providing new insight for the personalized treatment of HP-infected GC.
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http://dx.doi.org/10.3390/cancers14133276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265823PMC
July 2022

Label-Free and Noninvasive Single-Cell Characterization for the Viscoelastic Properties of Cryopreserved Human Red Blood Cells Using a Dielectrophoresis-On-a-Chip Approach.

Anal Chem 2022 07 6;94(28):10245-10255. Epub 2022 Jul 6.

Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei 230027, China.

Successful human red blood cell cryopreservation techniques have been gradually developed in recent decades, with great potential for use in clinical medicine and basic research. The mechanical properties of a single cell are important clues to reveal the physiological and pathological state of the red blood cell, but they have not been used to assess the physiological state of the cell after cryopreservation. Herein, we investigated the effects of cryopreservation processes on human red blood cell biomechanics by means of a microfluidic, label-free, synchronous, and nondestructive electrodeformation assay. We found that the effects of viscoelasticity of the red blood cell membrane between permeable and impermeable cryoprotectants were different. Our findings showed that high freeze-thaw recovery did not mean that the recovered cells had excellent viscoelasticity. The results demonstrate that single-cell viscoelasticity is an irreplaceable indicator for assessing the quality of the recovered cells and help us to deepen understanding of the cryoinjury mechanism. Assessment of the single cell viscoelasticity offers significant potential for application in optimizing the cryopreservation process and screening optimal cryoprotectants.
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http://dx.doi.org/10.1021/acs.analchem.2c01858DOI Listing
July 2022

MiR-4458-loaded gelatin nanospheres target COL11A1 for DDR2/SRC signaling pathway inactivation to suppress the progression of estrogen receptor-positive breast cancer.

Biomater Sci 2022 Aug 9;10(16):4596-4611. Epub 2022 Aug 9.

Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, PR China.

RNA interference is a promising way to treat cancer and the construction of a stable drug delivery system is critically important for its application. Gelatin nanospheres (GNs) comprise a biodegradable drug vehicle with excellent biocompatibility, but there are limited studies on its delivery and role in the stabilization of miRNA and siRNA. Breast cancer is the most diagnosed type of female cancer worldwide. Abnormal miRNA expression is closely related to the occurrence and progression of estrogen receptor-positive (ER+) breast cancer. In this study, miR-4458 was upregulated in ER+ breast cancer and could inhibit MCF-7 cell viability, colony formation, migration, and invasion. Collagen type XI alpha 1 (COL11A1) was identified as a directly interacting protein of miR-4458 and an important component of the extracellular matrix. High COL11A1 expression was positively correlated with poor prognosis, lower overall survival, disease-free survival, and a late tumor-node-metastasis stage. COL11A1 knockdown could inhibit MCF-7 cell migration and invasion. GNs were used to load a miR-4458 mimic or COL11A1 siRNA (si-COL11A1) to achieve sustained and controlled release in xenograft nude mice. Their tumor volume was decreased, tumor cell apoptosis was promoted, and hepatic metastasis was significantly inhibited. Moreover, the DDR2/SRC signaling pathway was inactivated after transfection with the miR-4458 mimic and si-COL11A1. In conclusion, GNs can be potentially used to deliver siRNA or miRNA, and miR-4458 and COL11A1 can be possible targets for ER+ breast cancer treatment.
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http://dx.doi.org/10.1039/d2bm00543cDOI Listing
August 2022

Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma.

Cancer Med 2022 Jul 4. Epub 2022 Jul 4.

Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin's Clinical Research Center for Cancer, National Clinical Research Center for Cancer, Tianjin, China.

Background: Esophageal squamous cell carcinoma (ESCC) has a dismal prognosis, and hypoxia plays a key role in metastasis and proliferation of ESCC. Thus, we aimed to develop a hypoxia-based gene signature to assist in the treatment decisions and prognosis.

Methods: We performed consensus clustering analysis on samples from GSE53625 dataset from the Gene Expression Omnibus (GEO) database and used weighted gene co-expression network analysis to filter out candidate modules, which were then intersected with differentially expressed genes from clustered subgroups to obtain hypoxia-related genes (HRGs). After that, the aforementioned genes were used to construct risk score models and validated in The Cancer Genome Atlas (TCGA) database and Cox regression analysis were used to construct a nomogram. Immunohistochemical was used to detect protein expression levels of relevant genes. Moreover, the relationship between risk scores and tumor microenvironment was explored.

Results: A hypoxia risk model containing six genes (PNPLA1, CARD18, IL-18, SLC37A2, ADAMTS18, and FAM83C) was constructed by screening key HRGs. Poorer prognosis in the high-risk group than in the low-risk group. And Cox regression analysis showed that risk score was an independent prognostic factor. The nomogram based on risk scores could well predict 1-, 3-, and 5-year survival. P53, Wnt, and hypoxia signaling pathways may be some regulatory mechanisms of hypoxia associated with the tumor microenvironment. In addition, we confirmed the high expression of BGN and low expression of IL-18 in ESCC tissues.

Conclusions: Our study determined the prognostic value of a 6-hypoxia gene signature and a prognostic model, providing potential prognostic predictors and therapeutic targets for ESCC.
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http://dx.doi.org/10.1002/cam4.5002DOI Listing
July 2022

Effects of dihydroartemisinin combined with cisplatin on proliferation, apoptosis and migration of HepG2 cells.

Oncol Lett 2022 Aug 22;24(2):275. Epub 2022 Jun 22.

Department of Medical Biology, School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.

Cisplatin (DDP) is a potent and widely applied chemotherapeutic agent. However, its clinical efficacy for the treatment of liver cancer is limited by adverse effects and the development of resistance. Combinatorial therapy may alleviate these issues. Dihydroartemisinin (DHA) is a first-generation derivative of artemisinin. The effects of DDP on liver cancer when applied in combination with DHA have not previously been studied. Therefore, the present study aimed to investigate the effects of DHA combined with DDP on HepG2 cells and their potential underlying molecular mechanisms. HepG2 cells were treated with different concentrations of DHA and/or DDP. Cell Counting Kit-8 assay was used to assess the cell viability. Cell proliferation and apoptosis were quantified using flow cytometry, acridine orange/ethidium bromide (AO/EB) fluorescent dual staining and the colony formation assay. Cell migration was quantified using the Transwell and wound healing assays. The HepG2 cell protein expression levels of Fas, Fas-associated death domain (FADD), procaspase-3, cleaved caspase-3, pro-caspase-8, cleaved caspase-8, Bax, Bcl-2, E-cadherin and N-cadherin, were detected via western blotting. Gelatin zymography was used to assess the levels of MMP-9 secreted by HepG2 cells into the supernatant. Following combined DHA and DDP treatment, the percentage of apoptotic cells was significantly increased, whereas cell proliferation and migration were significantly reduced, compared with cells treated with DDP only. DHA and DPP in combination significantly inhibited the expression of MMP-9, significantly increased the protein expression levels of Fas, FADD, Bax and E-cadherin, significantly increased the ratio of cleaved caspase-3 and cleaved caspase-8 to their precursor proteins and significantly decreased the protein expression levels of Bcl-2 and N-cadherin. The findings of the present study suggested that, DHA may confer synergistic effects with DDP in potentially promoting apoptosis and inhibiting the epithelial-mesenchymal transition for the treatment of liver cancer.
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http://dx.doi.org/10.3892/ol.2022.13395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247656PMC
August 2022

Nervous system manifestations related to COVID-19 and their possible mechanisms.

Brain Res Bull 2022 Sep 28;187:63-74. Epub 2022 Jun 28.

The College of Life Sciences and Medicine, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, PR China. Electronic address:

In December 2019, the novel coronavirus disease (COVID-19) due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection broke. With the gradual deepening understanding of SARS-CoV-2 and COVID-19, researchers and clinicians noticed that this disease is closely related to the nervous system and has complex effects on the central nervous system (CNS) and peripheral nervous system (PNS). In this review, we summarize the effects and mechanisms of SARS-CoV-2 on the nervous system, including the pathways of invasion, direct and indirect effects, and associated neuropsychiatric diseases, to deepen our knowledge and understanding of the relationship between COVID-19 and the nervous system.
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http://dx.doi.org/10.1016/j.brainresbull.2022.06.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236920PMC
September 2022

Evaporative water loss of 1.42 million global lakes.

Nat Commun 2022 06 28;13(1):3686. Epub 2022 Jun 28.

Zachry Department of Civil and Environmental Engineering, Texas A&M University, College Station, TX, 77843, USA.

The evaporative loss from global lakes (natural and artificial) is a critical component of the terrestrial water and energy balance. However, the evaporation volume of these water bodies-from the spatial distribution to the long-term trend-is as of yet unknown. Here, using satellite observations and modeling tools, we quantified the evaporation volume from 1.42 million global lakes from 1985 to 2018. We find that the long-term average lake evaporation is 1500 ± 150 km year and it has increased at a rate of 3.12 km year. The trend attributions include an increasing evaporation rate (58%), decreasing lake ice coverage (23%), and increasing lake surface area (19%). While only accounting for 5% of the global lake storage capacity, artificial lakes (i.e., reservoirs) contribute 16% to the evaporation volume. Our results underline the importance of using evaporation volume, rather than evaporation rate, as the primary index for assessing climatic impacts on lake systems.
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http://dx.doi.org/10.1038/s41467-022-31125-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240014PMC
June 2022

Definitive chemoradiotherapy versus neoadjuvant chemoradiotherapy followed by surgery in patients with locally advanced esophageal squamous cell carcinoma who achieved clinical complete response when induction chemoradiation finished: A phase II random.

Radiother Oncol 2022 Jun 25;174:1-7. Epub 2022 Jun 25.

Department of Radiation Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, China. Electronic address:

Background And Purpose: More than 40% of patients with esophageal squamous cell carcinoma (ESCC) exhibit pathological complete responses (pCR) after neoadjuvant chemoradiotherapy (nCRT), and theoretically, these patients may be cured by CRT and omit surgery. This prospectively randomized pilot study compared definitive chemoradiotherapy (dCRT) with nCRT in patients with locally advanced ESCC who achieved clinical complete responses (cCRs) to nCRT.

Materials And Methods: Single center, randomized, open phase 2 study of 256 patients with locally advanced ESCC enrolled between April 2016 and November 2018. Immediately when nCRT finished, patients enrolled underwent response evaluations within 1 week. Patients with cCR were randomly allocated to undergo surgery (arm A) or complete CRT up to the definitive radiation dose (arm B). The primary end point was 3-year disease-free survival (DFS).

Results: Finally, 71 patients were randomly assigned to the nCRT (n = 36) and dCRT (n = 35) arms. The median observation time was 35.7 months. The 3-year DFS rate was 56.43 % in arm A versus 54.73 % in arm B (hazard ratio [HR] = 0.862, 95 % confidence interval [CI] = 0.452 to 1.645, P = 0.652). The 3-year overall survival (OS) rates in arms A and B were 69.5 % and 62.3 % (HR = 0.824, 95 % CI = 403-1.688, P = 0.597), respectively.

Conclusions: According to our treatment response evaluation criteria, survival of the patients with cCR after nCRT was not significant different between nCRT group and dCRT group. An optimized response evaluation strategy soon after nCRT may guide next therapy decisions for patients with locally advanced ESCC.
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http://dx.doi.org/10.1016/j.radonc.2022.06.015DOI Listing
June 2022

Trends in Abdominal Obesity and Central Adiposity Measures by Dual-Energy X-Ray Absorptiometry Among US Children: 2011-2018.

Front Pediatr 2022 9;10:903413. Epub 2022 Jun 9.

Department of Maternal and Child Health, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objective: Previous studies that have reported trends on abdominal obesity among US children were usually based on anthropometric assessments. However, little is known about the recent trends in central adiposity measures by DXA and abdominal obesity since 2011-2012.

Study Design: A serial cross-sectional analysis of US population-weighted data among children from NHANES 2011 to 2018 was conducted.

Results: Between 2011-2012 and 2017-2018, there was a relatively stable trend among children aged 8-19 years in trunk fat and trunk fat percentage. During the same time periods, there were no significant changes in prevalence of abdominal obesity by waist circumference (18.6 vs. 21.1%) among those aged 2-19 years, and abdominal obesity by WHtR (34.1 vs. 36.2%) among those aged 6-19 years. However, a significant increase trend among boys aged 2-19 years was found in prevalence of abdominal obesity by waist circumference (16.1-22.7%; = 0.004). For Mexican American youth and non-Hispanic Asian boys, there is a significant increase in mean trunk fat percentage and waist circumference.

Conclusion: Between 2011-2012 and 2017-2018, there have been no significant changes in central adiposity measured by DXA and prevalence of abdominal obesity among US children. Our study further supports that there is an urgent need to improve their lifestyle to reduce abdominal obesity for US children, especially for Mexican American youth and non-Hispanic Asian boys.
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http://dx.doi.org/10.3389/fped.2022.903413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218269PMC
June 2022

The Influence of Annealing Temperature on the Morphology of Structures and the Mechanical Properties of Prequenching-Quenching and Partitioning Steel.

Materials (Basel) 2022 Jun 11;15(12). Epub 2022 Jun 11.

The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, China.

In this study, we comparatively study the microstructures and mechanical properties of prequenching-quenching and partitioning (QQ&P) and traditional Q&P samples at different annealing temperatures (intercritical annealing temperatures). When the annealing temperature is 780 °C, the ferrite and retained austenite in QQ&P samples with lath and blocky morphologies. The lath retained austenite is mainly distributed along the lath ferrite. As the annealing temperature increases, the lath ferrite recrystallizes and gradually grows into the blocky (equiaxed) shape, leading to a decrease in the lath retained austenite content. When the annealing temperature increases to 870 °C, the ferrite content decreases significantly, and the retained austenite is mainly blocky and thin film, distributed at the boundaries of prior austenite grains and between martensite laths, respectively. Different from QQ&P samples, the ferrite and retained austenite in Q&P samples are mainly blocky when the annealing temperature is 780 °C or 810 °C. When the annealing temperature is increased to 870 °C, the microstructures of the Q&P sample are basically the same as that of the QQ&P sample. The 780 °C-QQ&P sample and the 810 °C-QQ&P sample have higher total elongation and product of strength and elongations (PSEs) than their counterpart Q&P samples due to the fact that lath ferrite and retained austenite are conducive to carbon diffusion and carbon homogenization in austenite grains, thereby improving the thermal stability and volume fraction of the retained austenite. In addition, the lath structures can release local stress concentration and delay the formation of voids and microcracks. The difference of mechanical properties between QQ&P samples and Q&P samples decreases with the increase in the annealing temperature. The results show that the low annealing temperature combined with prequenching-Q&P heat treatments can significantly improve the elongation and PSE of Q&P steel.
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http://dx.doi.org/10.3390/ma15124156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229657PMC
June 2022

Experimental Study on the Cracking and Mechanical Properties of Lime Soil with Different Slaking Conditions of Newly Repaired Earthen City Walls.

Materials (Basel) 2022 Jun 11;15(12). Epub 2022 Jun 11.

School of Civil Engineering and Architecture, Henan University, Kaifeng 475004, China.

In this paper, a method to control the lime reaction by different slaking conditions is proposed to reduce the occurrence of cracks in newly repaired earthen city walls. The effects and mechanisms of the slaking time (0 h, 12 h, 24 h, 48 h and 72 h), lime content (10%, 15% and 20%), and moisture content (14%, 18% and 22%) on the cracking and mechanical properties of lime soil were analyzed by the test results of surface cracks, triaxial compression, particle gradation, pH value, X-ray diffraction and scanning electron microscope. The results show that proper slaking of lime soil specimens can reduce surface cracks and improve mechanical properties. After 12 h of appropriate slaking, the crack rate of the lime soil with 20% content decreased by 97.13%, the cohesion increased by 20.27%, and the internal friction angle decreased by 11.27%. However, the mechanical properties decreased when the slaking time was too long. After 72 h of slaking, the cohesion of 20% lime soil decreased by 8.21% and the internal friction angle increased by 2.82%. Further analysis shows that the appropriate slaking conditions can regulate the reaction rate and alkali environment, control the lime produced cementitious substances, improve the particle gradation and further reduce the occurrence of surface cracks. These results provide a basis for the restoration technology of newly repaired earthen city walls.
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http://dx.doi.org/10.3390/ma15124151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227263PMC
June 2022

Galunisertib attenuates progression of trauma-induced heterotopic ossification via blockage of Smad2/3 signaling in mice.

Eur J Pharmacol 2022 Aug 20;928:175109. Epub 2022 Jun 20.

Department of Hand Surgery, Wuxi 9th People's Hospital Affiliated to Soochow University, Wuxi, 214062, China. Electronic address:

Heterotopic ossification (HO) is the formation of bony tissues in the extraskeletal system. To date, no effective therapy has been developed for the treatment of HO, although increasing evidences have shown that inhibition of TGF-β signaling has potential as a new therapeutic approach for attenuating HO progression. Results from previous clinical trials have demonstrated that patients with malignant tumors exhibit excellent tolerability to Galunisertib, a TGF-β receptor I kinase inhibitor. However, its therapeutic potential in preventing HO and inhibitory effect on osteogenesis remain unclear. In this study, we demonstrated that intragastrical administration of Galunisertib, at a concentration as low as 10 mg/kg, was not only fairly effective in preventing HO development in a dose-dependent manner, but also generated a non-toxic response in a novel Achilles tendon puncture-induced traumatic HO model in mice. Moreover, Galunisertib treatment in the early phases of HO development, including the inflammatory and chondrogenic period, resulted in better therapeutic effects instead of eliminating already formed bony tissues. Mechanistically, Galunisertib suppressed the osteogenic differentiation capacity of tendon-derived stem cells (TDSCs) by interfering with the Smad2/3 signaling pathway, blocking the phosphorylation of Smad2/3 translocated from cytoplasm into the nucleus to regulate the expression of both osteogenesis-related transcription factors and related proteins. Results from in vivo experiments further validated Galunisertib's effect on HO attenuation, by intercepting the TGF-β/Smad2/3 signaling pathway. In conclusion, our findings demonstrated Galunisertib's potential as a prophylactic drug for the treatment of traumatic HO or other related diseases triggered by over-expressed TGF-β.
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http://dx.doi.org/10.1016/j.ejphar.2022.175109DOI Listing
August 2022

Parameterization of the ambient aerosol refractive index with source appointed chemical compositions.

Sci Total Environ 2022 Jun 21;842:156573. Epub 2022 Jun 21.

Department of Atmospheric and Oceanic Sciences, School of Physics, Peking University, Beijing 100871, China.

The refractive index of ambient aerosols is widely used in the climate model and remote sensing. Traditionally, the real part of the refractive index (RRI) of the ambient aerosol is calculated from the measured mass fraction of the main inorganic components with known refractive index, without full resolving the effects of variation in the RRI of organic components, which always contribute more than 50 % of the total aerosol mass. For the first time, the ambient aerosol RRI and the aerosol chemical components were measured concurrently at a suburban site Changping, in Beijing, China. Measurements results show that the ambient aerosol ranges between 1.57 and 1.71 with a mean value of 1.66. The mean mass fractions of organic aerosol (OA), nitrate, sulfate, ammonium, and chloride to total non-refractory aerosol loading are 43.1 %, 21.9 %, 21.6 %, 13.1 %, and 0.3 % respectively. Source appointment analysis of the organic aerosol show that the fossil fuel-related OA, cooking OA, biomass burning OA, less oxidized oxygenated OA and more oxidized OOA contributes 18.0 %, 11.2 %, 4.1 %, 39.9 %, 26.7 % to the total aerosol. A new parameterization scheme of the ambient aerosol RRI, which considers the source appointed OA, is proposed based on the concurrent measurements of RRI and chemical composition. The measured and parameterized RRI shows good consistency with a correlation coefficient of 0.79 and slope of 0.98. Our measurement results reveal that a significant deviation of the calculated RRI exists without considering the variation of the RRI of the aerosol organic component. The parametrization scheme is adopted and applicable in aerosol model for bettering estimating the corresponding optical and radiative effects.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156573DOI Listing
June 2022

Low Tumor Infiltrating Mast Cell Density Reveals Prognostic Benefit in Cervical Carcinoma.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221106530

Department of Medical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, Xinjiang, China.

Research on the role of mast cells (MCs) in cervical tumor immunity is more limited. Therefore, our study aimed to evaluate the prognostic value of MCs and their correlation with the immune microenvironment of cervical carcinoma (CC). The Cancer Genome Atlas (TCGA) data was utilized to obtain the degree of immune infiltration of MCs in CC. Meanwhile, this study retrospectively collected patient clinical characteristic data and tissue specimens to further verify the relevant conclusions. Mast cell density (MCD) was measured by the CIBERSORT algorithm in TCGA data and immunohistochemical staining of tryptase in CC tissues. Finally, differentially expressed genes (DEGs) of TCGA data were performed using "limma" packages and key gene modules were identified using the MCODE application in Cytoscape. The results showed MCs were diffusely distributed in CC tissues. Moreover, we found that low tumor-infiltrating MCD was beneficial for overall survival (OS) in the TCGA cohort. Consistent conclusions were also obtained in a clinical cohort. In addition, a total of 305 DEGs were analyzed between the high tumor-infiltrating MCD and low tumor-infiltrating MCD group. Seven key modules, a total of 34 genes, were screened through the MCODE plug-in, which was mainly related to inflammatory response and immune response and closely correlated with cytokines including CSF2, CCL20, IL1A, IL1B, and CXCL8. In short, high tumor-infiltration MCs in CC tissue was associated with worse OS in patients. Furthermore, MCs were closely related to cytokines in the tumor microenvironment, suggesting that they collectively played a role in the immune response of the tumor. Therefore, MCD may be a potential prognostic indicator and immunotherapy target of CC.
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http://dx.doi.org/10.1177/15330338221106530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228650PMC
June 2022

The transition between acute and chronic infections in light of energy control: a mathematical model of energy flow in response to infection.

J R Soc Interface 2022 06 22;19(191):20220206. Epub 2022 Jun 22.

Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Rebenring 56, 38106 Braunschweig, Germany.

Background: Different parts of an organism like the gut, endocrine, nervous and immune systems constantly exchange information. Understanding the pathogenesis of various systemic chronic diseases increasingly relies on understanding how these subsystems orchestrate their activities.

Methods: We started from the working hypothesis that energy is a fundamental quantity that governs activity levels of all subsystems and that interactions between subsystems control the distribution of energy according to acute needs. Based on physiological knowledge, we constructed a mathematical model for the energy flow between subsystems and analysed the resulting organismal responses to infections.

Results: The model reproduces common behaviour in acute infections and suggests several host parameters that modulate infection duration and therapeutic responsiveness. Moreover, the model allows the formulation of conditions for the induction of chronic infections and predicts that alterations in energy released from fat can lead to the transition from clearance of acute infections to a chronic inflammatory state.

Impact: These results suggest a fundamental role for brain and fat in controlling immune response through systemic energy control. In particular, it suggests that lipolysis resistance, which is known to be involved in obesity and ageing, might be a survival programme for coping with chronic infections.
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http://dx.doi.org/10.1098/rsif.2022.0206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214282PMC
June 2022

Autonomous Bionanorobots a Cage-Shaped Silsesquioxane Vehicle for Heavy Metal Detoxification.

ACS Appl Mater Interfaces 2022 Jun 17;14(25):29238-29249. Epub 2022 Jun 17.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, and College of Materials Science and Engineering & Research Center for Analysis and Measurement, Donghua University, Shanghai 201620, China.

Nanorobots hold great promise for integrated drug delivery systems that are responsive to molecular triggers. Herein, we successfully developed an automatic smart bionanorobot that has transport capability and recognizes and removes zinc ions from poisoned cells based on nanoscale polyhedral oligomeric silsesquioxane molecules. This intelligent bionanorobot can easily move inside and outside the cell and find zinc ions owing to its highly selective recognition to zinc ions and high cell permeability, especially the well-combined high penetration and strong binding energy. More importantly, it was also found that this intelligent bionanorobot can restore round HeLa cells to a normal fusiform cell morphology following high-concentration zinc treatment and does not interfere with cell proliferation and division. It was also shown by experiments that the bionanorobot can inhibit persistent enlargement of the liver caused by zinc ion poisoning.
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http://dx.doi.org/10.1021/acsami.2c05736DOI Listing
June 2022
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