Publications by authors named "Gang Zhao"

1,346 Publications

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Bioactive compounds, health benefits, and industrial applications of Tartary buckwheat ().

Crit Rev Food Sci Nutr 2021 Jul 19:1-17. Epub 2021 Jul 19.

Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo - Ourense Campus, Ourense, Spain.

Tartary buckwheat belongs to the family Polygonaceae, which is a traditionally edible and medicinal plant. Due to its various bioactive compounds, the consumption of Tartary buckwheat is correlated to a wide range of health benefits, and increasing attention has been paid to its potential as a functional food. This review summarizes the main bioactive compounds and important bioactivities and health benefits of Tartary buckwheat, emphasizing its protective effects on metabolic diseases and relevant molecular mechanisms. Tartary buckwheat contains a wide range of bioactive compounds, such as flavonoids, phenolic acids, triterpenoids, phenylpropanoid glycosides, bioactive polysaccharides, and bioactive proteins and peptides, as well as D-chiro-inositol and its derivatives. Consumption of Tartary buckwheat and Tartary buckwheat-enriched products is linked to multiple health benefits, , antioxidant, anti-inflammatory, antihyperlipidemic, anticancer, antidiabetic, antiobesity, antihypertensive, and hepatoprotective activities. Especially, clinical studies indicate that Tartary buckwheat exhibits remarkable antidiabetic activities. Various tartary buckwheat -based foods presenting major health benefits as fat and blood glucose-lowering agents have been commercialized. Additionally, to address the safety concerns, , allergic reactions, heavy metal and mycotoxin contaminations, the quality control standards for Tartary buckwheat and its products should be drafted and completed in the future.
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http://dx.doi.org/10.1080/10408398.2021.1952161DOI Listing
July 2021

Insights on the interaction mechanism of exemestane to three digestive enzymes by multi-spectroscopy and molecular docking.

Int J Biol Macromol 2021 Jul 15;187:54-65. Epub 2021 Jul 15.

Department of Chemistry, School of Pharmacy, the Fourth Military Medical University, Xi'an 710032, PR China. Electronic address:

Exemestane is an irreversible steroidal aromatase inhibitor, typically used to treat breast cancer. As an anti-tumor drug, exemestane has more obvious side effects on the gastrointestinal tract. The purpose of this work is to investigate the combination of exemestane with three important digestive enzymes including pepsin (Pep), trypsin (Try) and α-Chymotrypsin (α-ChT) so as to analyze the mechanism of the gastrointestinal adverse effects causing by exemestane binding. Enzyme activity experiment showed that the enzyme activity of Pep was decreased in the presence of exemestane. Fluorescence spectra revealed that exemestane formed stable complexes with digestive enzymes, and the quenching mechanism of drug-digestive enzymes interaction were all static quenching. The binding constants of Pep, Try and α-ChT at 298 K were 2.34 × 10, 1.45 × 10, and 2.05 × 10 M, respectively. Synchronous fluorescence and 3D fluorescence spectroscopy showed that the conformation of exemestane was slightly changed after combining with digestive enzymes, and non-radiative energy transfer occurred. Circular dichroism results indicated that exemestane could change the secondary structure of digestive enzymes via increase the α-helix content and decrease in the β-sheet content. Thermodynamic parameters (ΔH, ΔS, and ΔG) revealed that exemestane interacted with α-ChT through electrostatic force, and the binding force with Pep and Try was van der Waals interactions and hydrogen, which was basically consistent with the molecular docking results.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.079DOI Listing
July 2021

Enantioselectivity switch in asymmetric Michael addition reactions using phosphonium salts.

Org Biomol Chem 2021 Jul;19(28):6334-6340

Department of Chemistry, University of Science and Technology of China, Hefei 230026, Anhui, China. and Center for Excellence in Molecular Synthesis, Key Laboratory of Synthetic Chemistry of Natural Substances, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 LingLing Road, Shanghai 200032, China.

Efficient access to two enantiomers of one chiral compound is critical for the discovery of drugs. However, it is still a challenging problem owing to the difficulty in obtaining two enantiomers of one chiral catalyst. Here, we report a general method to obtain both enantiomeric products via fine tuning the hydrogen-bonding interactions of phosphonium salts. Amino acid derived phosphonium salts and dipeptide derived phosphonium salts exhibited different properties for controlling the transition state, which could efficiently promote the Michael addition reaction to give opposite configurations of products with high yields and enantioselectivities. Preliminary investigations on the mechanism of the reaction and applications of the products were also performed.
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http://dx.doi.org/10.1039/d1ob01027aDOI Listing
July 2021

Comparative Secretome Analyses of Virulent and Attenuated Strains Revealed MbovP0145 as a Promising Diagnostic Biomarker.

Front Vet Sci 2021 18;8:666769. Epub 2021 Jun 18.

The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Mycoplasmas are successful pathogens both in humans as well as in animals. In cattle, () is known to be responsible for serious health complications, including pneumonia, mastitis, and arthritis. However, pathogenesis remains unclear. Secreted proteins of could influence infection and modify host defense signaling pathways after they enter their extracellular space in the host micro-environment. Therefore, this study was aimed to compare the secretomes of HB0801 virulent (P1) and attenuated (P150) strains and identify potential pathogenesis-related secreted proteins and biomarkers. The cells of P1 and P150 strains were grown in pleuropneumonia-like organism medium to log phase and then transferred to phosphate-buffered saline for 2 h. Then, the supernatant was analyzed by using label-free quantitative proteomics, and 477 potential secreted proteins were identified. Combined with the bioinformatics prediction, we found that 178 proteins were commonly secreted by the P1 and P150 strains, and 49 of them were encoded by mycoplasmal core genes. Additionally, 79 proteins were found to have a different abundance between the P1 and P150 strains. Among these proteins, 34 were more abundant and uniquely expressed in P1, indicating a possible association with the virulence of . Three differentially secreted proteins, MbovP0145, MbovP0725, and MbovP0174, as well as one equally secreted protein, MbovP0481, as positive control and one protein of inner membrane, MbovP0310, as negative control were, respectively, cloned, expressed, and evaluated for antigenicity, subcellular location, and the secretion nature with their mouse antisera by western blotting and colony immunoblotting assay. Among them, MbovP0145 was confirmed to be more secreted by P1 than P150 strain, highly reactive with the antisera from naturally infected and P1 experimentally infected cattle but not with the P150 vaccinated calves, indicating its potential as a diagnostic antigen. In conclusion, these findings may represent the most extensive compilation of potentially secreted proteins in mycoplasma species and the largest number of differentially secreted proteins between the virulent and attenuated strains to date and provide new insights into pathogenesis and diagnosis.
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http://dx.doi.org/10.3389/fvets.2021.666769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249566PMC
June 2021

Multi-responsive nanofibers composite gel for local drug delivery to inhibit recurrence of glioma after operation.

J Nanobiotechnology 2021 Jul 3;19(1):198. Epub 2021 Jul 3.

Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, 221002, China.

Background: The postoperative recurrence of malignant gliomas has presented a clinical conundrum currently. Worse, there is no standard treatment for these recurrent tumours. Therefore, novel promising methods of clinical treatment are urgently needed.

Methods: In this study, we synthesized reactive oxygen species (ROS)-triggered poly(propylene sulfide)60 (PPS60) mixed with matrix metalloproteinases (MMPs)-responsive triglycerol monostearate (T) lipids and TMZ. The mixed solution could self-assemble at 50 ℃ to generate hydrogels with MMPs- and ROS-responsiveness. We explored whether the T/PPS + TMZ hydrogel could achieve the MMP- and ROS-responsive delivery of TMZ and exert anti-glioma regrowth effects in vitro and in vivo. These results demonstrated that the T/PPS + TMZ hydrogel significantly improved the curative effect of TMZ to inhibit postsurgical recurrent glioma.

Results: The results confirmed the responsive release of TMZ encapsulated in the T/PPS + TMZ hydrogel, and the hydrogel showed excellent performance against glioma in an incomplete glioma operation model, which indicated that the T/PPS + TMZ hydrogel effectively inhibited the growth of recurrent glioma.

Conclusion: In summary, we successfully developed injectable MMPs- and ROS-responsive hydrogels that could achieve the sustained release of TMZ in the surgical cavity to inhibit local recurrent glioma after surgery.
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http://dx.doi.org/10.1186/s12951-021-00943-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255008PMC
July 2021

Frequency stabilization of a quantum cascade laser by weak resonant feedback from a Fabry-Perot cavity.

Opt Lett 2021 Jul;46(13):3057-3060

Frequency-stabilized mid-infrared lasers are valuable tools for precision molecular spectroscopy. However, their implementation remains limited by complicated stabilization schemes. Here we achieve optical self-locking of a quantum cascade laser to the resonant leak-out field of a highly mode-matched two-mirror cavity. The result is a simple approach to achieving stable frequencies from high-powered mid-infrared lasers. For short time scales (<0.1), we report a linewidth reduction factor of 3×10 to a linewidth of 12 Hz. Furthermore, we demonstrate two-photon cavity-enhanced absorption spectroscopy of an overtone transition near a wavelength of 4.53 µm.
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http://dx.doi.org/10.1364/OL.427083DOI Listing
July 2021

Epidemiology and Survival of Patients With Brainstem Gliomas: A Population-Based Study Using the SEER Database.

Front Oncol 2021 11;11:692097. Epub 2021 Jun 11.

Department of Neurosurgery, First Hospital of Jilin University, Changchun, China.

Background: Brainstem glioma is a primary glial tumor that arises from the midbrain, pons, and medulla. The objective of this study was to determine the population-based epidemiology, incidence, and outcomes of brainstem gliomas.

Methods: The data pertaining to patients with brainstem gliomas diagnosed between 2004 and 2016 were extracted from the SEER database. Descriptive analyses were conducted to evaluate the distribution and tumor-related characteristics of patients with brainstem gliomas. The possible prognostic indicators were analyzed by Kaplan-Meier curves and a Cox proportional hazards model.

Results: The age-adjusted incidence rate was 0.311 cases per 100,000 person-years between 2004 and 2016. A total of 3387 cases of brainstem gliomas were included in our study. Most of the patients were white and diagnosed at 5-9 years of age. The most common diagnosis confirmed by histological review was ependymoma/anaplastic ependymoma. The median survival time was 24 months. Patients with tumors less than 3 cm in size had a better prognosis. Surgery was effective at improving overall survival. There was no evidence that radiotherapy and chemotherapy improved overall survival.

Conclusion: Brainstem gliomas can be diagnosed at any age. Ependymoma/anaplastic ependymoma is the most common pathological diagnosis. The prognosis is poor, and timely diagnosis and surgery are effective at improving the prognosis. We suggest that more attention should be given to the treatment of patients with brainstem gliomas.
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http://dx.doi.org/10.3389/fonc.2021.692097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237753PMC
June 2021

The locoregional adiponectin and its synergistic antitumor effect with HIF-1α blockade in TSCC.

Oral Dis 2021 Jun 26. Epub 2021 Jun 26.

Department of Medical Biology, School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, China.

Adiponectin (APN) is a kind of endogenous anti-tumor adipocytokine, which exerts its function by binding to its receptors (AdipoR1 and AdipoR2). However, hyperadiponectinemia is found in some pathophysiological processes without significant protective effect, which indicates the existence of APN resistance. Here, we aimed to investigate the locoregional expression of APN in tongue squamous cell carcinoma (TSCC) tissues, and to explore the potential regulatory mechanism of APN resistance under hypoxia. Consequently, we found that the protein expression of APN and AdipoR1, but not AdipoR2, was upregulated in the early stage of TSCC and after hypoxic treatment ex vivo and in vitro. Knockdown of HIF-1α decreased the level of APN and AdipoR1, and simultaneously, HIF-1α was identified as transcriptor of the APN. Intriguingly, a regenerative feedback of HIF-1α was unexpectedly detected after application of recombinant globular APN (gAPN), which most likely contributed to the APN resistance. Furthermore, HIF-1α blockade combined with gAPN has a prominent synergistic antitumor effect, which suggested an effective amelioration in APN resistance. In all, our study revealed the possible mechanism of APN resistance under hypoxia and provides a promising strategy of bi-target treatment with APN and HIF-1α for TSCC therapy.
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http://dx.doi.org/10.1111/odi.13948DOI Listing
June 2021

Multiple calcifying fibrous tumor of the pleura: A case report.

Thorac Cancer 2021 Jun 26. Epub 2021 Jun 26.

Department of Lung Cancer, Tianjin Lung Cancer Center, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin, China.

Calcifying fibrous tumor of the pleura (CFTP) is a rare benign tumor of the thoracic cavity. Due to the low incidence of CFPT, it is prone to be misdiagnosed because intraoperative analysis of frozen section is a challenge for pathologists. At present, it is difficult to distinguish this tumor from other benign thoracic tumors based on radiographic features. Therefore, surgical resection is the best method for definite diagnosis and treatment.
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http://dx.doi.org/10.1111/1759-7714.14064DOI Listing
June 2021

Neoadjuvant chemoimmunotherapy in resectable stage IIIA/IIIB non-small cell lung cancer.

Transl Lung Cancer Res 2021 May;10(5):2193-2204

Department of Lung Cancer, Tianjin Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: A small proportion of patients with non-small cell lung cancer (NSCLC) experience objective clinical benefit after neoadjuvant programmed cell death 1 (PD-1) blockade. A neoadjuvant therapeutic regimen combining immune checkpoint blockade with chemotherapy might improve the treatment effect, but such a regimen has not been tested in patients with resectable stage IIIA/IIIB NSCLC.

Methods: A retrospective study of 35 patients with resectable stage IIIA and IIIB NSCLC who were treated with neoadjuvant chemoimmunotherapy (NCIO) was performed. Patients were evaluated for pathological complete response (pCR), major pathologic response (MPR), safety, and feasibility. The correlations of pathologic response with various clinical factors were studied to identify predictors of pathological response.

Results: NCIO was associated with few immediate adverse events. NCIO did not delay planned surgery and led to a pCR rate of 51.43% and an MPR rate of 74.29% for the primary tumor. No association was observed between programmed death-ligand 1 (PD-L1) expression before NCIO and the pathologic response (Pearson's r=-0.071; P=0.685). However, a significant difference was observed in pathological response in patients with intracavitary and extracavitary tumors (P<0.05). Patients with intracavitary type had a higher pCR (76.47% 31.58%) and MPR (100% 50.00%) rate than patients with extracavitary type (Pearson's r=0.7280; P=0.0009).

Conclusions: NCIO was associated with few side effects, did not delay surgery, and achieved a pCR in 51.43% and MPR in 74.29% of resected tumors. No significant correlation was found between pathologic response and PD-L1 expression. While the intracavitary and extracavitary tumors type T was predictive of the pathological response to NCIO.
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http://dx.doi.org/10.21037/tlcr-21-329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182703PMC
May 2021

The particle phase state during the biomass burning events.

Sci Total Environ 2021 Jun 1;792:148035. Epub 2021 Jun 1.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

The phase state of biomass burning aerosols (BBA) remains largely unclear, impeding our understanding of their effects on air quality, climate and human health, due to its profound roles in mass transfer between gaseous and particulate phase. In this study, the phase state of BBA was investigated by measuring the particle rebound fraction ƒ combining field observations and laboratory experiments. We found that both ambient and laboratory-generated BBA had unexpectedly lower rebound fraction ƒ (<0.6) under the dry conditions (RH = 20-50%), indicating that BBA were in non-solid state at such low RH. This was obviously different from the secondary organic aerosols (SOA) derived from the oxidation of both anthropogenic and biogenic volatile organic compounds, typically with a rebound fraction ƒ larger than 0.8 at RH below 50%. Therefore, we proposed that the diffusion coefficient of gaseous molecular in the bulk of BBA might be much higher than SOA under the dry conditions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148035DOI Listing
June 2021

Improvement of nickel-cobalt-based supercapacitors energy storage performance by modification of elements.

J Colloid Interface Sci 2021 Jun 13;602:712-720. Epub 2021 Jun 13.

School of Physics and Technology, University of Jinan, Shandong 250022, PR China. Electronic address:

Hybrid supercapacitors have the advantages of fast charging and discharging and long service life, which are an efficient and practical energy storage device. Therefore, the design of hybrid supercapacitors is the focus of current research. In this paper, the silver modified spinel NiCoS nanorods (AgS-NiCoS/CF) are synthesized by an efficient and economical method, which has excellent electrochemical performance. The AgS-NiCoS/CF shows a high specific capacity of 179.7 mAh g at current density of 1 A g, and excellent rate capability (capacitance retention of ~87% at 20 A g). The corresponding AgS-NiCoS/CF//AC/CF hybrid supercapacitor is assembled by AgS-NiCoS/CF as the positive electrode, which can provide an energy density of 35.978 Wh kg at a high-power density of 800 W kg and has significant cyclic stability (~80% of the initial capacitor after ~9600 cycles). Therefore, AgS-NiCoS/CF material is a promising electrode material that can be applied to hybrid supercapacitors.
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http://dx.doi.org/10.1016/j.jcis.2021.06.063DOI Listing
June 2021

MiR-223 promotes pyroptosis of enteritis cells through activating NF-κB signalling pathway by targeting SNIP1 in inflammatory bowel disease.

Autoimmunity 2021 Jun 21:1-11. Epub 2021 Jun 21.

Department of Anorectum, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Inflammatory bowel disease (IBD) is a common inflammation-related intestinal disease. Studies have shown that excessive pyroptosis of intestinal cells is involved in the development of IBD. However, the regulatory mechanism of pyroptosis in IBD remains unclear. Here, our study purposed to clarify the underlying regulatory mechanism of miR-223 to promote pyroptosis in IBD.MiR-223 and Smad Nuclear Interacting Protein 1 (SNIP1) expression in colon tissues collected from IBD patients and healthy volunteers were evaluated using qRT-PCR. Cell viability and pyroptosis were evaluated by CCK8 and flow cytometry assay, respectively. Pyroptosis-related proteins and nuclear factor κB (NF-κB) signals were determined by WB. Dual-luciferase reporter gene assay was employed to investigate the binding relationship between miR-223 and SNIP1.MiR-223 was significantly upregulated in IBD colon tissues and cell models, while SNIP1 was significantly decreased. Silence of miR-223 markedly enhanced cell viability and inhibited pyroptosis in the IBD cell model. MiR-223 could bind to 3'-UTR of SNIP1 and SNIP1 could activate NF-κB signalling pathway. Further rescued experiment found that knockdown of SNIP1 dramatically abolished the bio-effects mediated by miR-223 silence on the cell viability and pyroptosis of the IBD cell model. Likewise, the inactivation of NF-κB signalling markedly weakened the regulatory roles of SNIP1 downregulation in the IBD cell model. Besides, inhibition of NF-κB signalling attenuated the pyroptosis-promoting effect of overexpressing miR-223.Our data suggested that miR-223 activated the NF-κB pathway targeting SNIP1, thus promoting the process of cell pyroptosis, and ultimately participating in the pathogenesis of IBD.
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http://dx.doi.org/10.1080/08916934.2021.1940973DOI Listing
June 2021

Characterization of the complete chloroplast genome of L. var. with phylogenetic analysis.

Mitochondrial DNA B Resour 2021 Jun 3;6(7):1852-1854. Epub 2021 Jun 3.

Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industralization, School of Food and Biological Engineering, Chengdu University, Chengdu, P. R. China.

In the present study, the complete chloroplast genome of L. var. was sequenced, assembled and compared with closely related species. The chloroplast genome of L. var. was composed of 84 protein-coding genes (PCG), 8 ribosomal RNA (rRNA) genes, and 38 transfer RNA (tRNA) genes. The chloroplast genome is 136,485 bp in size, with the GC content of 38.32%. Phylogenetic analysis based on the combined chloroplast gene dataset indicated that the L. var. exhibited a close relationship with subsp. and subsp. .
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http://dx.doi.org/10.1080/23802359.2021.1935343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183546PMC
June 2021

Incidence Density and Predictors of Multidrug-Resistant Tuberculosis Among Individuals With Previous Tuberculosis History: A 15-Year Retrospective Cohort Study.

Front Public Health 2021 28;9:644347. Epub 2021 May 28.

Hangzhou Center for Disease Control and Prevention, Hangzhou, China.

To date, too little attention has been paid to monitoring and estimating the risk of incident multidrug-resistant tuberculosis (MDR-TB) among individuals with a previous tuberculosis history (PTBH). The purpose of this study was to assess the incidence of and risk factors for MDR-TB in those individuals. Between 2005 and 2020, a large, retrospective, population-based cohort study was performed in Hangzhou, China. A multivariable Cox regression model was used to evaluate independent predictors of incident MDR-TB among individuals with PTBH. The incidence density of MDR-TB was 22.6 per 1,000 person-years (95% confidence level and an interval of 20.9-24.3) for individuals with PTBH. The incidence of MDR-TB increased significantly in individuals who • were under 60 years old. • were male. • had a history of direct contact. • came from low-income families. • worked in high-risk occupations. • lived in rural areas. • had a retreatment TB history. • had an unfavorable outcome in their previous treatment ( < 0.05). In addition, we found that the following factors were significantly linked to the MDR-TB risk among individuals with PTBH ( < 0.05): • sociodemographic factors such as the 21-30 and 31-40 year age groups, or a history of direct contact. • clinical factors like passive modes of TB case finding (PMTCF), human immunodeficiency virus infection, unfavorable treatment outcomes, retreated TB history, non-standardized treatment regimens of retreatment TB patients, and duration of pulmonary cavities (DPC). • microbiological factors, such as duration of positive sputum culture. We also found that the 21-30 year age group, low family income, and PMTCF were significantly linked to incident MDR-TB only in males with PTBH, whilst the 41-50 year age group, extended treatment course, and DPC were significantly associated with female MDR-TB only. The incidence of MDR-TB was high, with a higher rate among subjects with a history of direct contact and unfavorable treatment outcomes. There was a gender difference in the incidence density and risk factors of MDR-TB among individuals with PTBH. Long-term monitoring and gender-specific risk-factor modifications should be given to individuals with PTBH.
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http://dx.doi.org/10.3389/fpubh.2021.644347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193499PMC
June 2021

MicroRNA miR-509-3p inhibit metastasis and epithelial-mesenchymal transition in hepatocellular carcinoma.

Bioengineered 2021 12;12(1):2263-2273

School of Stomatology, Jiamusi University, Jiamusi, China.

Our study seeks to obtain data which help to assess the impacts and related mechanisms of microRNA miR-509-3p in hepatocellular carcinoma (HCC). We found that the expression of miR-509-3p was down-regulated and Twist was up-regulated in HCC tissues and cell lines (HepG2, HCCLM3, Bel7402, and SMMC7721) compared with the adjacent normal tissues and normal human hepatocyte (L02). Moreover, cell proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) in HepG2 and HCCLM3 cells were appeared to be markedly suppressed by overexpressed miR-509-3p. Overexpression of miR-509-3p also performed inhibition of the growth and metastasis in vivo. In addition, miR-509-3p could target and inhibit Twist expression, and it could further reverse the tumor promotion by Twist in HCC. All in all, miR-509-3p overexpression causes inhibition of the proliferation, migration, invasion and EMT of HCC cells by negatively regulating Twist, thereby suppressing HCC development and metastasis.
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http://dx.doi.org/10.1080/21655979.2021.1932210DOI Listing
December 2021

lncRNA IUR upregulates miR-34a to inhibit pancreatic adenocarcinoma cell migratory and invasive abilities.

Oncol Lett 2021 Jul 29;22(1):567. Epub 2021 May 29.

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

The long non-coding RNA (lncRNA) imatinib-upregulated (IUR) has been recently reported as a tumor suppressor in leukemia. Preliminary microarray data revealed a downregulation of IUR in pancreatic adenocarcinoma (PAAD) and a positive correlation with microRNA-34a (miR-34a) expression. The present study aimed to investigate the role of IUR in PAAD. This study included samples from 58 patients with PAAD and the PAAD cell lines Capan-2 and HPAC. Reverse transcription quantitative PCR was performed to determine gene expression levels. Cell transfections were carried out to assess gene interactions between IUR, miR-34a and CD44. Transwell assays were performed to explore the effects of transfections on cell invasive and migratory abilities. The results demonstrated that IUR was downregulated in PAAD tissue compared with adjacent non-tumor tissue samples and that low expression levels of IUR correlated with poor survival in patients with PAAD. In PAAD tissue samples, the expression of IUR positively correlated with miR-34a expression but negatively correlated with CD44 expression, which is a target of miR-34a. In PAAD cells, overexpression of IUR resulted in miR-34a upregulation and CD44 downregulation. miR-34a overexpression did not affect the expression of IUR but downregulated CD44. In PAAD cells, overexpression of IUR and miR-34a led to decreased invasive and migratory abilities. However, CD44 overexpression played an opposite role and attenuated the effects of IUR and miR-34a overexpression. In conclusion, the results from this study demonstrated that IUR may upregulate miR-34a expression in order to inhibit PAAD cell migration and invasion by downregulating CD44.
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http://dx.doi.org/10.3892/ol.2021.12828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185698PMC
July 2021

Cocatalysts from types, preparation to applications in the field of photocatalysis.

Authors:
Gang Zhao Xijin Xu

Nanoscale 2021 Jun;13(24):10649-10667

Laboratory of Functional Micro-nano Material and Device, School of Physics and Technology, University of Jinan, Jinan, Shandong, P. R. China.

With the rapid development of society, the burden of energy and the environment is becoming more and more serious. Photocatalytic hydrogen production, the photosynthesis of organic fuel, and the photodegradation of pollutants are three effective ways to reduce these burdens using semiconductor photocatalysts. To improve the reaction efficiency of photocatalysts, a small amount of cocatalyst is often added when photocatalysts participate in the synthesis or decomposition reaction. The addition of this small amount of cocatalyst is like a finishing touch, significantly increasing the activity of the photocatalysts. However, in our common study of photocatalysis, we often pay attention to the study of photocatalysts but ignore the study of cocatalysts. Herein, we summarize the recent application research on cocatalysts in the field of photocatalysis, starting from the types, preparation methods, and reaction mechanisms among others, to remind researchers of the matters needing attention when using cocatalysts.
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http://dx.doi.org/10.1039/d1nr02464gDOI Listing
June 2021

Polyelectrolyte multilayer composite coating on 316 L stainless steel for controlled release of dual growth factors accelerating restoration of bone defects.

Mater Sci Eng C Mater Biol Appl 2021 Jul 14;126:112187. Epub 2021 May 14.

Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan. Electronic address:

A composite coating of polyelectrolyte multilayers (PEMs) consisting of collagen, a chitosan barrier, and poly-γ-glutamic acid was fabricated using a spin coating technique to investigate and overcome the limited osseointegration capacity of 316 L stainless steel (316 L SS). To further enhance the biocompatibility, bone morphogenetic protein 2 (BMP-2) and basic fibroblast growth factor-2 (FGF-2) were loaded separately as dual growth factors, allowing for progressive drug release following the natural process of bone regeneration. The first burst release of FGF-2 triggered the proliferation of surrounding cells, and the subsequent release of BMP-2 stimulated their differentiation. The microstructure, surface potential, hardness, reduced Young's modulus, and wettability were assessed using scanning electron microscopy, nanoindentation, and water contact angle. The formation of apatite layers after immersion in simulated body fluid confirmed the bioactivity of this PEM. PEMs loaded with BMP-2 and FGF-2 showed a long sustained release of growth factors for up to 48 days. The biological properties were studied in vitro with rat bone mesenchymal stem cells (rBMSCs) and in vivo using a rat critical-sized calvarial defect model. PEMs loaded with growth factors further stimulated the proliferation and osteogenic differentiation of rBMSCs and the histology results indicated that new bone tissues could directly grow onto the PEMs. These findings suggest that PEM composite coating possesses significant potential for surface modification and long-term drug release of metallic implants to assist with bone restoration.
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http://dx.doi.org/10.1016/j.msec.2021.112187DOI Listing
July 2021

Preoperative C-Reactive Protein/Albumin Ratio is a Prognostic Indicator for Survival in Surgically Treated Gastrointestinal Stromal Tumors: A Retrospective Cohort Study.

Cancer Manag Res 2021 24;13:4155-4167. Epub 2021 May 24.

Department of Gastrointestinal Surgery, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Background: Systemic inflammation and malnutrition may promote tumor progression. C-reactive protein/albumin ratio (CAR) is linked to the poor long-term survival of several malignant tumors.

Purpose: To explore the predictive value of CAR in gastrointestinal stromal tumors (GISTs).

Methods: A retrospective study was conducted on 325 patients with primary GIST surgically treated with curative intent from 2009 to 2018. The cut-off point of CAR was set using X-tile software. Kaplan-Meier method and multivariate Cox regression model were used to study the prognostic value of CAR. The time-dependent receiver operating characteristic curve (tROC) was drawn, and the prognostic accuracy of CAR, Glasgow prognostic score (GPS), and National Institute of Health (NIH) risk classification was compared by the area under the curve (AUC).

Results: The best cut-off point of CAR was 0.55. Increased CAR was associated with the location of the lower digestive tract, larger tumor size, higher mitotic index, higher NIH risk classification, lower ALB, higher CRP, and higher GPS (all p<0.05). Multivariable analysis revealed that CAR (hazard ratio [HR] 2.598, 95% confidence interval [CI] 1.385-4.874; p=0.003) was an independent predictor of overall survival. Additionally, the AUC of CAR was lower than that of NIH risk classification at 2 years (0.601 vs. 0.775, p=0.002) and 5 years (0.629 vs 0.735, p=0.069). However, the AUC of NIH risk classification significantly increased (2-year OS 0.801, p=0.251; 5-year OS 0.777, p=0.011) when combined with CAR.

Conclusion: CAR is a new independent predictor of poor survival in patients with GIST. CAR combined with NIH risk classification can effectively improve the performance of prognosis prediction.
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http://dx.doi.org/10.2147/CMAR.S307873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163582PMC
May 2021

miR-506-loaded gelatin nanospheres target PENK and inactivate the ERK/Fos signaling pathway to suppress triple-negative breast cancer aggressiveness.

Mol Carcinog 2021 Aug 1;60(8):538-555. Epub 2021 Jun 1.

Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.

Triple-negative breast cancer (TNBC) is the most malignant subtype of breast cancer. Some microRNAs (miRNAs) were abnormally expressed in TNBC, and they are closely related to the occurrence and progression of TNBC. Here, we found that miR-506 was significantly downregulated in TNBC and relatively lower miR-506 expression predicted a poorer prognosis. Moreover, we found that miR-506 could inhibit MDA-MB-231 cell viability, colony formation, migration, and invasion, and suppress the ERK/Fos oncogenic signaling pathway through upregulating its direct target protein proenkephalin (PENK). Therefore, miR-506 was proposed as a nucleic acid drug for TNBC therapy. However, miRNA is unstable in vivo, which limiting its application as a therapeutic drug via conventional oral or injected therapies. Here, a gelatin nanosphere (GN) delivery system was applied for the first time to load exogenous miRNA. Exogenous miR-506 mimic was loaded on GNs and injected into the in situ TNBC animal model, and the miR-506 could achieve sustained and controlled release. The results confirmed that overexpression of miR-506 and PENK in vivo through loading on GNs inhibited in situ triple-negative breast tumor growth and metastasis significantly in the xenograft model. Moreover, we indicated that the ERK/Fos signaling pathway was intensively inactivated after overexpression of miR-506 and PENK both in vitro and in vivo, which was further validated by the ERK1/2-specific inhibitor SCH772984. In conclusion, this study demonstrates that miR-506-loaded GNs have great potential in anti-TNBC aggressiveness therapy.
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http://dx.doi.org/10.1002/mc.23310DOI Listing
August 2021

Drug preconcentration and direct quantification in biofluids using 3D-Printed paper cartridge.

Biosens Bioelectron 2021 Oct 13;189:113266. Epub 2021 May 13.

School of Life Science and Technology, Xidian University, Xi'an, 710126, Shaanxi, PR China; Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address:

Drug detection in biofluids has always been great importance for clinical diagnosis. Many detection technologies such as chromatography-mass spectrometry, have been applied to the detection of drugs. However, these technologies required multi-step operations, including complicated and time-consuming pretreatment processes and operations of bulky detection instruments, significantly limiting development of drug detection in clinical diagnosis. Herein, a portable 3D-printed paper cartridge was fabricated for fast sample preconcentration and direct drugs quantitative detection in biofluids by a portable Raman spectrometer. This cartridge contained both paper tip with silver nanowires to preconcentrate samples and achieve surface-enhanced Raman Scattering (SERS) measurement, and 3D-printed cartridge to build enclosed environment for the improvement of detection, which cost only one dollar. The preconcentration ability of the cartridge was up to 18.13-fold fluorescence enhancement and compared to the non-preconcentration method, it achieved 9.93-fold improvement of SERS performance. The anticancer drug of epirubicin hydrochloride, cyclophosphamide and their mixtures were quantitatively detected in the bovine serum or artificial urine. The integrated detection procedure required only 1 h, including sample pretreatment and preconcentration, drying, SERS measurements, and quantification analysis. This 3D-printed paper cartridge constituted a portable detection platform that would be potentially a practical and point-of-care detection tool for drug preconcentration and quantification on the clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2021.113266DOI Listing
October 2021

Controlled Release of Cryoprotectants by Near-Infrared Irradiation for Improved Cell Cryopreservation.

ACS Biomater Sci Eng 2021 06 24;7(6):2520-2529. Epub 2021 May 24.

Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.

Cryopreservation is essential to store living cells and tissues for future use while maintaining the proper levels of cell functions. The use of cryoprotective agents (CPAs) to inhibit intracellular ice formation during cryopreservation is vital for cell survival, but the addition and removal of CPAs and ice recrystallization during rewarming will cause fatal injury to cells. The conventional CPA loading and unloading methods generate osmotic shocks and cause mechanical injury to biological samples, and the conventional method of rewarming using a water bath also leads to ice recrystallization and devitrification. A new CPA-loaded microparticle-based method for loading and photothermal rewarming under near-infrared (NIR) laser irradiation was proposed to overcome these difficulties. We have successfully achieved the controlled release of CPAs (2 M EG, 2 M PG, and 0.5 M trehalose) with a graphene oxide (GO, 0.04% w/v) core from a 1.5% (w/v) sodium alginate shell to the human umbilical vein endothelial cells (HUVECs) within 60 s using NIR laser irradiation (808 nm Lasever at 5000 mW/cm) and successfully recovered the CPA-loaded cells with 0.04% (w/v) GO in 8-10 s using the same NIR irradiation. The results show that this method achieved 25% higher viability of HUVECs compared to the conventional method. In short, this study proposes a new approach for achieving controlled CPA loading to cells with a photothermal-induced strategy for cell cryopreservation.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00171DOI Listing
June 2021

Iterative stripe artifact correction framework for TOF-MRA.

Comput Biol Med 2021 07 11;134:104456. Epub 2021 May 11.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China; Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; Department of Radiation Oncology, Stanford University, Stanford, CA, 94305, USA. Electronic address:

The purpose of this study is to develop a practical stripe artifacts correction framework on three-dimensional (3-D) time-of-flight magnetic resonance angiography (TOF-MRA) obtained by multiple overlapping thin slab acquisitions (MOTSA) technology. In this work, the stripe artifacts in TOF-MRA were considered as a part of image texture. To separate the image structure and the texture, the relative total variation (RTV) was firstly employed to smooth the TOF-MRA for generating the template image with fewer image textures. Then a residual image was generated, which was the difference between the template image and the raw TOF-MRA. The residual image was served as the image texture, which contained the image details and stripe artifacts. Then, we obtained the artifact image from the residual image via a filter in a specific direction since the image artifacts appeared as stripes. The image details were then produced from the difference between the artifact image and the image texture. To produce the corrected images, we finally compensated the image details to the RTV smoothing image. The proposed method was continued until the stripe artifacts during the iteration vary as little as possible. The digital phantom and the real patients' TOF-MRA were used to test the approach. The spatial uniformity was increased from 74% to 82% and the structural similarity was improved from 86% to 98% in the digital phantom test by using the proposed algorithm. Our approach proved to be highly successful in eliminating stripe artifacts in real patient data tests while retaining image details. The proposed iterative framework on TOF-MRA stripe artifact correction is effective and appealing for enhancing the imaging performance of multi-slab 3-D acquisitions.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104456DOI Listing
July 2021

Inhibition of osteoclastogenesis by histone deacetylase inhibitor Quisinostat protects mice against titanium particle-induced bone loss.

Eur J Pharmacol 2021 Aug 15;904:174176. Epub 2021 May 15.

Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, 215007, China; Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China. Electronic address:

Periprosthetic osteolysis (PPO) and subsequent aseptic loosening are major long-term complications after total joint arthroplasty and have become the first causes for further revision surgery. Since PPO is primarily caused by excessive bone resorption stimulated by released wear particles, osteoclast-targeted therapy is considered to be of great potential for PPO prevention and treatment. Accumulating evidences indicated that inhibition of histone deacetylases (HDACs) may represent a novel approach to suppress osteoclast differentiation. However, different inhibitors of HDACs were shown to exhibit distinct safety profiles and efficacy in inhibiting osteoclastogenesis. Quisinostat (Qst) is a hydroxamate-based histone deacetylase inhibitor, and exerts potent anti-cancer activity. However, its effect on osteoclastogenesis and its therapeutic potential in preventing PPO are still unclear. In this study, we found that Qst suppressed RANKL-induced production of TRAP-positive mature osteoclasts, expression of osteoclast-specific genes, formation of F-actin rings, and bone resorption activity at a nanomolar concentration as low as 2 nM in vitro. Furthermore, we found that as low as 30 μg/kg of Qst was sufficient to exert preventive effect on titanium particle-induced osteolysis in the murine calvarial osteolysis model. Mechanistically, we found that Qst suppressed osteoclastogenesis by interfering with NF-κB and c-Fos/NFATc1 pathways. Thus, our study revealed that Qst may serve as a potential therapeutic agent for prevention and treatment of PPO and other osteoclast-mediated diseases.
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http://dx.doi.org/10.1016/j.ejphar.2021.174176DOI Listing
August 2021

Active control of terahertz waves based on p-Si hybrid PIT metasurface device under avalanche breakdown.

Opt Express 2021 Apr;29(8):12712-12722

Active control of terahertz waves is a critical application for terahertz devices. Silicon is widely used in large-scale integrated circuit and optoelectronic devices, and also shows great potential in the terahertz field. In this paper, a p-Si hybrid metasurface device is proposed and its terahertz characteristics under avalanche breakdown effect is investigated. In the study, a plasmon-induced transparency (PIT) effect caused by the near-field coupling of the bright mode and the dark mode is observed in the transmission spectrum. Due to avalanche breakdown effect, the resonance of the PIT metamaterial disappears as the current increased. Carriers existed in the interface between the metasurface and substrate result to a dipole resonance suppression. When the current continues increasing, the maximal modulation depth can reach up to 99.9%, caused by the avalanche effect of p-Si. Experimental results demonstrate that the avalanche breakdown p-Si can achieve a performance modulation depth, bringing much more possibilities for terahertz devices.
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http://dx.doi.org/10.1364/OE.421820DOI Listing
April 2021

Near-Infrared Light-, Magneto-, and pH-Responsive GO-FeO/Poly(-isopropylacrylamide)/alginate Nanocomposite Hydrogel Microcapsules for Controlled Drug Release.

Langmuir 2021 05 30;37(18):5522-5530. Epub 2021 Apr 30.

Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.

Responsive hydrogels have found widespread applications in biomedical science and engineering fields, especially for drug delivery. Despite the superior performance of responsive hydrogels, challenges still exist in drug-delivery efficiency when environmental stimuli are weak. Recently, the demand in the design of hydrogel-based drug delivery systems has stimulated considerable interest in the search for new strategies, for instance, the application of nanocomposite hydrogels for reinforcing the versatility and flexibility in controlled drug delivery. In this study, a novel and effective nanocomposite hydrogel microcapsule drug delivery system, which is composed of poly(-isopropylacrylamide) (PNIPAM) and alginate interpenetrating polymer and GO-FeO nanomaterials, is developed to achieve NIR light-, magneto-, and pH-responsive drug release. The GO-FeO nanomaterials embedded in the interpenetrating polymer enable the PNIPAM hydrogel deswelling by raising temperature above the lower critical solution temperature under NIR light and alternating magnetic field, thus accelerating the release of doxorubicin. In addition, the introduction of alginate into PNIPAM hydrogels endows nanocomposite hydrogels (NCHs) with quick gelation property, enhanced mechanical property, and pH-responsive performance. The in vitro cytotoxicity assay confirmed that the NCH platform can effectively kill the cancer cells. This novel multiresponsive drug delivery system holds great promise for the treatment of diseases.
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http://dx.doi.org/10.1021/acs.langmuir.1c00207DOI Listing
May 2021

The correlation between tumor-associated macrophage infiltration and progression in cervical carcinoma.

Biosci Rep 2021 May;41(5)

State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, China.

Tumor microenvironment (TME) plays a particularly important role in the progression, invasion and metastasis of cervical carcinoma (CC). Tumor-associated macrophages (TAMs) are significant components of the tumor microenvironment in CC. However, the results of studies on the correlation between TAMs and progression in CC are still controversial. This research aimed to investigate the relationship between TAMs infiltration and progression in CC. A total of 100 patients with CC were included in the study. The correlation between TAMs and clinicopathologic features was studied. Besides, a systematic literature search was conducted from legitimate electronic databases to specifically evaluate the role of TAMs in TME of cervical carcinoma. In the meta-analysis, high stromal CD68+ TAMs density was relevant to lymph node metastasis (WMD = 11.89, 95% CI: 5.30-18.47). At the same time, CD163+ M2 TAM density was associated with lymph node metastasis (OR = 2.42, 95% CI: 1.09-5.37; WMD = 39.37, 95% CI: 28.25-50.49) and FIGO stage (WMD = -33.60, 95% CI: -45.04 to -22.16). This was further confirmed in the experimental study of 100 tissues of cervical cancer. It supported a critical role of TAMs as a prospective predictor of cervical cancer. In conclusion, CD68+ TAM and CD163+ M2 TAM infiltration in CC were associated with tumor progression. And CD163+ M2 TAM infiltration was associated with more advanced FIGO stage and lymph node metastasis in CC.
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http://dx.doi.org/10.1042/BSR20203145DOI Listing
May 2021

By Regulating the NLRP3 Inflammasome Can Reduce the Release of Inflammatory Factors in the Co-Culture Model of Tuberculosis H37Ra Strain and Rat Microglia.

Front Cell Infect Microbiol 2021 13;11:637769. Epub 2021 Apr 13.

The College of Life Sciences and Medicine, Northwest University, Xi'an, China.

Objective: Tuberculosis infection of the Central Nervous System can cause severe inflammation in microglia, and NLRP3 inflammasome is also an important source of inflammation in microglia. Therefore, in this study, we used a co-culture model of rat microglia and tuberculosis H37Ra strain to explore the influence of tuberculosis infection on the NLRP3 inflammasome in microglia and its regulation mechanism.

Methods: We cultured primary microglia from SD rats and co-cultured with tuberculosis H37Ra strain for 4 hours to establish a co-culture model. At the same time, MCC950, Z-YVAD-FMK, BAY-11-7082, Dexamethasone, RU486, BzATP, BBG and extracellular high potassium environment were used to intervene the co-cultivation process. Subsequently, western blot, real-time PCR, ELISA and other methods were used to detect the changes of NLRP3 inflammasome-related molecules in microglia.

Results: After co-cultivation, the NLRP3 inflammasomes in microglia were activated and released a large amount of IL-18 and IL-1β. By regulating NLRP3 inflammasome complex, caspase-1, NF-κB and P2X7R during the co-culture process, it could effectively reduce the release of IL-18 and IL-1β, and the mortality of microglia.

Conclusion: Our results indicate that the NLRP3 inflammasome pathway is an important part of the inflammatory response of microglia caused by tuberculosis infection. By intervening the NLRP3 inflammasome pathway, it can significantly reduce the inflammatory response and mortality of microglia during the tuberculosis H37Ra strain infection. This research can help us further understand the inflammatory response mechanism of the central nervous system during tuberculosis infection and improve its treatment.
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http://dx.doi.org/10.3389/fcimb.2021.637769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078893PMC
July 2021