Publications by authors named "Gang Yang"

560 Publications

Correlation between the prognosis of chronic hepatitis B treated with interferon and the gene polymorphism of CGRP and its receptor.

Infect Genet Evol 2021 Jun 9:104968. Epub 2021 Jun 9.

Department of Epidemiology and Statistics, School of Public Health, Hebei Medical University, Hebei Key Laboratory of Environment and Human health, Shi Jiazhuang, PR China. Electronic address:

Objective: To investigate the relationship of the polymorphism of two gene loci (CGRP rs155209 and RAMP1 rs3754701) with the prognosis of interferon therapy for chronic hepatitis B (CHB) by case-control study.

Materials And Methods: 317 CHB patients using interferon for the first time alone were collected in northern China and peripheral blood samples were obtained, the single-nucleotide polymorphisms (SNPs) in CGRP rs155209 and RAMP1 rs3754701 were genotyped by MALDI-TOF-MS. Univariate and multivariate logistic regression methods were used to assess the correlation between the prognosis of CHB treated with interferon and the gene polymorphism of CGRP rs155209 and RAMP1 rs3754701.

Results: The study clearly demonstrated the relevance of the polymorphism CGRP rs155209 and RAMP1 rs3754701 with DNA response and ALT response. The CHB patients with CGRP rs155209C were less prone to develop DNA response (CT vs TT: OR = 0.157, 95% CI = 0.085-0.291, P < 0.001; CC vs TT: OR = 0.132, 95% CI = 0.060-0.289, P < 0.001), and they also were less likely to develop ALT response (CT vs TT: OR = 0.530, 95% CI = 0.323-0.869, P = 0.012). Moreover, the CHB patients with RAMP1 rs3754701T allele were more prone to develop DNA response (AT vs AA:OR = 2.061, 95%CI = 1.237-3.435, P = 0.006; TT vs AA: OR = 5.676, 95% CI = 1.247-25.837, P = 0.025), and they also more likely to develop ALT response (AT vs AA: OR = 1.766, 95% CI = 1.098-2.840, P = 0.019).

Conclusion: Our study revealed that the polymorphisms of CGRP rs155209 and RAMP1 rs3754701 were associated with the prognosis of interferon therapy for CHB in the Han Chinese population, and RAMP1 rs3754701T was a protective factor for ALT response and DNA response, but CGRP rs155209C carriers were less prone to DNA and ALT responses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.meegid.2021.104968DOI Listing
June 2021

Survey of Dental Clinicians for Attitude and Use of Torque-Limiting Devices.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):538-545

Purpose: The purpose of this study was to survey practicing clinicians and determine if differences existed concerning their use of torque-limiting devices (TLDs) and screw-tightening protocols, comparing this with existing universal industry standards.

Materials And Methods: A nine-question survey was administered with 428 dentists providing data for three specific areas: (1) demographic information-TLD ownership, device age, frequency of use, and observations of screw loosening; (2) recognition information-calibration, reading measurements of the TLD, and the meaning of preload; (3) usage information-screw-tightening protocols and effect of speed during actioning of the TLD. Data collection was compared with industry standards for use of hand torque tools including ISO-6789 1,2:2017 and related texts pertaining to screw fastener protocols.

Results: The beam-type TLD was the most popular; however, 33% surveyed used it incorrectly. Most TLDs being used were older than 1 year, with only 6% calibrated. Forty-eight percent observed screw loosening less than once per year, while 44% reported three or more occurrences per year. A similar number used the TLD for implant placement and abutment screw tightening. Screw-tightening protocols varied. Preload was not understood by the majority of those surveyed.

Conclusion: Dentistry does not appear to adhere to the protocols and standards recommended by other industries that also rely on screw-fastening mechanisms and TLDs. Further education and training appears to be warranted in this area of implant dentistry to reduce the risks of screw-associated complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11607/jomi.8590DOI Listing
June 2021

Changes of serum IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 in COVID-19 patients.

Int J Clin Pract 2021 Jun 9:e14462. Epub 2021 Jun 9.

The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, Postal Code: 410007, China.

Background And Purpose: Studies have shown that some cytokines in COVID-19 patients were elevated. This study aims to assess whether IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 serve as potential diagnostic biomarkers of COVID-19.

Methods: The above serum cytokines in COVID-19 patients and non COVID-19 patients were detected by ELISA, SARS-CoV-2 IgM and IgG were detected by chemiluminescence method. Independent-samples Mann-Whitney U-test was utilized to compare cytokines levels in different groups and course, Levene T-test, T'-test were utilized to compare they in different genders, and Spearman Correlation test was utilized to analyze the correlation between the cytokines levels with ages, SARS-CoV-2 IgG and IgM.

Results: Serum levels of IL-10, IL-1β, MCP-1, TNF-α and IL-4 in COVID-19 patients were significantly higher than those in non-COVID-19 patients, while IL-6 were only significantly higher than in healthy people, IP-10 were significantly lower than in other diseases patients. AUCs of COVID-19 diagnosed by IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 were 0.735, 0.775, 0.595, 0.821, 0.848, 0.387 and 0.682, respectively. In COVID-19 patients' serum, the levels of IL-10 and MCP-1 of male had noticeably higher than those of female, and all cytokines were significantly positively correlated with age, IL-1β and IL-4 were significantly negatively correlated with SARS-CoV-2 IgM, while IL-10, IL-1β, IL-6, TNF-α and IP-10 were significantly negatively correlated with SARS-CoV-2 IgG. IL-10 on 43-56 days were significantly lower than at 29-42 days, TNF-α at 15-42 days were significantly higher than at 0-14 days, IP-10 at 0-14 days were the highest, IL-4 at 29-42 days were significant higher than on 0-14 days.

Conclusions: The detection of IL-10, IL-1 β, IL-6, MCP-1, TNF-α and IL-4 would assist the clinical study of COVID-19, and IP-10 may be the cytokine `of early elevation in COVID-19 patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/ijcp.14462DOI Listing
June 2021

Heptaphylline suppresses the proliferation and migration of human bladder cancer cells via induction of intrinsic apoptosis, autophagy and inhibition of β-catenin signalling pathway.

J BUON 2021 Mar-Apr;26(2):646

Department of Urology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200050, China.

Purpose: Heptaphylline has been shown to suppress the growth of different types of cancer cells. Nonetheless, the anticancer effects of Heptaphylline have not been examined against human bladder cancer cells. Against this backdrop, this study was undertaken to investigate the anticancer effects of the carbazole alkaloid Heptaphylline against human bladder cancer cells.

Methods: Proliferation rate was determined by MTT assay. Apoptosis was demonstrated by DAPI and annexin V/propidium iodide (PI) assay. Electron microscopy was used for autophagy detection. Western blot analysis was used to determine protein expression.

Results: The results showed that Heptaphylline suppressed the proliferation of the RT4 bladder cancer cells and exhibited an IC50 of 25 µM. The toxic effects of Heptaphylline were comparatively lower on the normal Hs172.T cells, as evidenced from the IC50 of 95 µM. The wound healing assay showed that Heptaphylline suppressed the migration of the RT4 bladder cancer cells. The DAPI and annexin V/PI staining showed that Heptaphylline induced apoptosis in the RT4 bladder cancer cells which was also accompanied by enhancement in the cleavage of PARP, caspase-3 and caspase-9. Additionally, Heptaphylline caused increase in Bax and decrease in Bcl-2 expression. Electron microscopic analysis showed that Heptaphylline also caused autophagy in the RT4 cells which was associated with increase in LC3, Atg5, Atg7 and Beclin-1 expression and decrease in p62 expression. This molecule also blocked the β-catenin signalling pathway in the RT4 bladder cancer cells.

Conclusion: Taken together, Heptaphylline suppressed the proliferation of the bladder cancer cells and may prove beneficial in the bladder cancer treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2021

Revealing the complexity of distinct manganese species-protein interactions through multi-spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 21;260:119981. Epub 2021 May 21.

College of Chemistry and Chemical Engineering, Bohai University, Jinzhou 121013, PR China.

The effect of manganese (Mn) on protein conformation is closely related to its chemical species. To further realize the behavior of different species of Mn in vivo, this study is designed to analyze the separate and simultaneous interactions of Mn(ii) and Mn(iii) with bovine serum albumin (BSA) using multi-spectroscopy. The results demonstrated that the interaction of Mn(ii) or Mn(iii) with BSA is a process of static quenching and Mn(iii) formed a more stable complex. The binding constants and thermodynamic constants indicated that a 1:1 complex was formed between Mn(ii)/Mn(iii) and BSA through a moderate binding force, and hydrophobic interaction played an important role in the binding. UV-Vis spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy results revealed that the conformation changes in BSA induced by Mn(ii)/Mn(iii) binding. The results of the ternary systems suggested that both Mn species interfered the interaction of the other with BSA. The conformation of BSA may change more to adapt to the simultaneous binding to Mn (ii) and Mn (iii) when two Mn species coexist.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119981DOI Listing
May 2021

Guided Relaxation-Based Virtual Reality for Acute Postoperative Pain and Anxiety: A Pilot Study in a Pediatric Population.

J Med Internet Res 2021 May 4. Epub 2021 May 4.

Cincinnati Childrens Hospital Medical Center, 3333 Burnet AvenueMLC 2001, Cincinnati, US.

Background: Distraction-based therapies, such as virtual reality (VR), have been used to reduce pain during acutely painful procedures. However, distraction alone cannot produce the prolonged pain reduction required to manage sustained postoperative pain. Integration of VR with other pain reducing therapies, like guided relaxation, may enhance its clinical impact.

Objective: The goal of this pilot study was to assess the association of a single guided relaxation-based VR (VR-GR) session with a reduction in postoperative pain and anxiety in children. We also explored whether pain catastrophizing and anxiety sensitivity influenced this association.

Methods: A total of 51 children and adolescents (7-21 years) with postoperative pain followed by the Acute Pain Service at Cincinnati Children's Hospital were recruited over an 8-month period to undergo a single VR-GR session. Prior to VR, patients completed pain catastrophizing (PCS-C) and anxiety sensitivity (CASI) questionnaires. The primary outcome was change in pain intensity following VR-GR (immediately, 15, and 30 minutes). Secondary outcomes included changes in pain unpleasantness and anxiety.

Results: VR-GR decreased pain intensity immediately (p < 0.001) and 30 minutes (p = 0.04) after the VR session, but not at 15 minutes (p = 0.16) post-session. Reductions in pain unpleasantness were observed during all time intervals (p < 0.001 at all intervals). Anxiety was reduced immediately (p = 0.02) but not at 15- (p = 0.08) or 30- (p = 0.30) minutes following VR-GR. Patients with higher CASI reported greater reductions in pain intensity (p = 0.04) and unpleasantness (p = 0.01) following VR-GR. Pain catastrophizing was not associated with changes in pain and anxiety.

Conclusions: A single, short VR-GR session showed transient reductions in pain intensity, pain unpleasantness, and anxiety in children and adolescents with acute postoperative pain. These results encourage a future randomized clinical trial assessing efficacy of VR-GR.

Clinicaltrial: Clinical Trials ID: NCT04556747.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2196/26328DOI Listing
May 2021

A study of the correlations between IVIM-DWI parameters and the histologic differentiation of hepatocellular carcinoma.

Sci Rep 2021 May 17;11(1):10392. Epub 2021 May 17.

Medical Imaging Key Laboratory of Sichuan Province, Medical Research Center, Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China.

The present study aimed to investigate the value of intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) in the preoperative prediction of the histologic differentiation of hepatocellular carcinoma (HCC). Seventy HCC patients were scanned with a 3.0 T magnetic resonance scanner. The values of apparent diffusion coefficient (ADC), slow apparent diffusion coefficient (D), fast apparent diffusion coefficient (D*), and the fraction of the fast apparent diffusion coefficient (f) were measured. Analysis of variance was used to compare the differences in parameters between groups with different degrees of histologic differentiation. p < 0.05 was considered statistically significant. Receiver operating characteristic (ROC) curves were used to analyse the efficacy of IVIM-DWI parameters for predicting the histologic differentiation of HCC. The ADC and D values for well, moderately and poorly differentiated HCC were 1.35 ± 0.17 × 10 mm/s, 1.16 ± 0.17 × 10 mm/s, 0.98 ± 0.21 × 10 mm/s, and 1.06 ± 0.15 × 10 mm/s, 0.88 ± 0.16 × 10 mm/s, 0.76 ± 0.18 × 10 mm/s, respectively, and all differences were significant. The D* and f values of the three groups were 32.87 ± 14.70 × 10 mm/s, 41.68 ± 17.90 × 10 mm/s, 34.54 ± 18.60 × 10 mm/s and 0.22 ± 0.07, 0.23 ± 0.08, 0.18 ± 0.07, respectively, with no significant difference. When the cut-off values of ADC and D were 1.25 × 10 mm/s and 0.97 × 10 mm/s, respectively, their diagnostic sensitivities and specificities for distinguishing well differentiated HCC from moderately differentiated and poorly differentiated HCC were 73.3%, 85.5%, 86.7%, and 78.2%, and their areas under the ROC curve were 0.821 and 0.841, respectively. ADC and D values can be used preoperatively to predict the degree of histologic differentiation in HCC, and the D value has better diagnostic value.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-89784-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129092PMC
May 2021

Elucidating the effects of TiO nanoparticles on the toxicity and accumulation of Cu in soybean plants (Glycine max L.).

Ecotoxicol Environ Saf 2021 Aug 11;219:112312. Epub 2021 May 11.

College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, PR China. Electronic address:

Copper (Cu) pollution is common in the soil. Due to the widespread application of TiO NPs, there is a high propensity for the co-occurrence of TiO nanoparticles (NPs) and Cu in agricultural soils. It is therefore imperative to evaluate the joint effects of TiO NPs and Cu on crops. In this study, the mutual effects of TiO NPs and Cu on their toxicity and accumulation in soybean seedlings and on their fates in a hydroponic system were determined. When Cu was at levels of 1 and 2 mg/L, the co-occurring TiO NPs at a non-toxic concentration (10 mg/L) significantly enhanced the toxicity and accumulation of Cu and Ti in soybeans, and inhibited the translocation of Cu from soybean roots to shoots. However, when the Cu concentration for co-exposure was ≥ 5 mg/L, such mutual effects disappeared. The amount of Cu ions adsorbed onto TiO NPs after 48 h of co-exposure gradually increased from 31 to 118 mg/g when the Cu concentration was increased from 1 to 20 mg/L. The aggregation and sedimentation of TiO NPs were significantly increased after 48 h of co-exposure with the Cu at a concentration higher than 5 mg/L, as compared to the single TiO NPs exposure. The increasing aggregation and sedimentation might reduce the bioavailability of TiO NPs associated with the adsorbed Cu to soybeans, and consequently alleviate or even neutralize the enhanced toxicity and accumulation of Cu in soybeans exerted by the co-existing TiO NPs. Our results thus suggest that consideration of the impact of TiO NPs on the phytotoxicity of heavy metals, and specifically Cu, needs to be interpreted with care, and highlight the importance of integrating the interaction and fates of TiO NPs and metals into their risk assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112312DOI Listing
August 2021

Assessment of the effects of four crosslinking agents on gelatin hydrogel for myocardial tissue engineering applications.

Biomed Mater 2021 May 26;16(4). Epub 2021 May 26.

College of Biomedical Engineering, Sichuan University, Chengdu 610065, Sichuan, People's Republic of China.

Cardiomyocyte (CM) transplantation is a promising option for regenerating infarcted myocardium. However, poor cell survival and residence rates reduce the efficacy of cell transplantation. Gelatin (GA) hydrogel as a frequently-used cell carrier is a possible approach to increase the survival rate of CMs. In this study, microbial transglutaminase (mTG) and chemical crosslinkers glutaraldehyde, genipin, and 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide were employed to prepare GA hydrogels. The mechanical properties and degradation characteristics of these hydrogels were then evaluated. Neonatal rat CMs (NRCMs) were isolated and inoculated on the surface of these hydrogels or encapsulated in mTG-hydrogels. Cellular growth morphology and beating behavior were observed. Cellular viability and immunofluorescence were analyzed. Intracellular Catransient and membrane potential propagation were detected using fluorescence dyes (Fluo-3 and di-4-ANEPPS, respectively). Results showed that the chemical crosslinkers exhibited high cytotoxicity and resulted in high rates of cell death. By contrast, mTG-hydrogels showed excellent cell compatibility. The CMs cultured in mTG-hydrogels for a week expressed CM maturation markers. The NRCMs begun independently beating on the third day of culture, and their beating synchronized after a week of culture. Furthermore, intracellular Catransient events with periodicity were observed. In conclusion, the novel mTG-crosslinked GA hydrogel synthesized herein has good biocompatibility, and it supports CM adhesion, growth, and maturation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1748-605X/abfff2DOI Listing
May 2021

The clinical management of extralobar pulmonary sequestration in children.

Pediatr Pulmonol 2021 Apr 30. Epub 2021 Apr 30.

Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Introduction: The treatment of extralobar pulmonary sequestration (ELS) remains divergent. This study aims to demonstrate the characters of ELS in children for optimal clinical management in the future.

Material And Methods: A retrospective analysis was conducted for ELS patients' treatment in our center from January 2013 to April 2020.

Results: In total, 85 patients were included, containing 70 upper-diaphragmatic, 7 intra-diaphragmatic, and 8 infra-diaphragmatic ELS. Eight patients' pathology results showing inflammation without symptoms preoperation and two patients had chest pain for torsion. All the upper-diaphragmatic and intra-diaphragmatic ELS patients accepted thoracoscopic surgery resection. The intraoperative operation time and blood loss volume of intra-diaphragmatic ELS were significantly more than that of the upper-diaphragmatic (40.14 ± 9.92  vs. 23.07 ± 6.79 min; 9.29 ± 3.45  vs. 3.18 ± 4.94 ml; all p < .05). No chest tubes were inserted in both subgroups. No complications were found in the postoperative follow-up of operative ELS patients at least 3 months. A total of eight infra-diaphragmatic ELS patients except for one (7/8) had conservative therapy and follow-up by the outpatient clinic or phone call eventually. There were no symptoms occurring and no size increasing of observation infra-diaphragmatic ELS.

Conclusions: The ELS has the potential risk of infection and torsion in this study. Thoracoscopic surgery might be optimal management of upper-diaphragmatic ELS for its minimal invasion and low perioperative risks, which could be developed into a day operation with safe and quick recovery. The intra-diaphragmatic and infra-diaphragmatic ELS need a larger sample size and multiple center data to get a better management approach.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ppul.25433DOI Listing
April 2021

CDC20 promotes the progression of hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.

Mol Med Rep 2021 Jul 28;24(1). Epub 2021 Apr 28.

Department of Hepatocellular Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637007, P.R. China.

Hepatocellular carcinoma (HCC) is a type of primary liver cancer, which is associated with high mortality. HCC is one of the most common malignant tumors worldwide. Cell division cycle 20 (CDC20) has been reported to be associated with the development of various malignant tumors and epithelial‑mesenchymal transition (EMT) has been reported to be involved in the malignant metastasis of HCC. Therefore, the present study hypothesized that CDC20 may participate in the malignant biological behavior of HCC via EMT. The present study analyzed the expression levels of CDC20 in HCC and the association between CDC20 and poor prognosis. Furthermore, the effects of CDC20 on the proliferation, invasion and migration of HCC cells were examined using proliferation, migration and invasion assays. Finally, alterations in EMT were analyzed. The results revealed that CDC20 was highly expressed in HCC and HCC cell lines (P<0.05), and its high expression level was significantly associated with poor prognosis in patients with HCC (P<0.05). CDC20 silencing inhibited the proliferation, migration and invasion of HCC cells. Furthermore, CDC20 silencing increased the expression levels of E‑cadherin, and decreased the expression levels of N‑cadherin, vimentin and Ki‑67. In conclusion, the present study reported that CDC20 may be a novel therapeutic target in HCC and CDC20 could promote the progression of HCC by regulating EMT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2021.12122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127032PMC
July 2021

γ-Cyclodextrin metal-organic framework as a carrier to deliver triptolide for the treatment of hepatocellular carcinoma.

Drug Deliv Transl Res 2021 Apr 15. Epub 2021 Apr 15.

Department of Pharmacy, Shanghai 9Th People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 280 Mohe Road, Shanghai, 201999, China.

Triptolide (TPL) has been employed to treat hepatocellular carcinoma (HCC). However, the poor water solubility of TPL restricts its applications. Therefore, we prepared TPL-loaded cyclodextrin-based metal-organic framework ([email protected]) to improve the solubility and bioavailability of TPL, thus enhancing the anti-tumor effect on HCC. The BET surface and the pore size of [email protected] were 10.4 m·g and 1.1 nm, respectively. The results of XRD indicated that TPL in [email protected] was encapsuled. [email protected] showed a slower release than free TPL in vitro. Moreover, the CD-MOF improved the bioavailability of TPL. [email protected] showed slightly higher, but statistically significant, anti-tumor efficacy in vitro and in vivo compared to free TPL. In addition, [email protected] exhibited a modest improvement of the anti-tumor effects, which may be associated to the enhanced in vivo absorption. Overall, these findings suggested the potential CD-MOF as oral drug delivery carriers for anti-tumor drugs. The process of TPL loading into CD-MOF and its enhanced oral bioavailability and anti-tumor activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13346-021-00978-7DOI Listing
April 2021

Descriptive statistics of dataset from the meta-analysis and meta-regression analysis on prognostic significance of pre-treatment systemic hemato-immunological indices of cervical cancer patients.

Data Brief 2021 Apr 3;35:106925. Epub 2021 Mar 3.

Department of Obstetrics and Gynecology, Daping Hospital (Army Medical Center of PLA), Army Medical University, #10 Changjiang Branch Road, Yuzhong District, Chongqing 400038, People's Republic of China.

In this study, we perform a meta-analysis and meta-regression analysis for the article entitled "Prognostic value of systemic hemato-immunological indices in uterine cervical cancer: A systemic review, meta-analysis, and meta-regression of observational studies." [1] We implemented quantitative meta-analyses and time series meta-regression analysis to determine whether systemic hemato-immunological indices, such as neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocyte ratio (LMR), thrombocyte-to-lymphocyte ratio (TLR), and C-reactive protein/albumin ratio (CAR) are associated with an increased risk of cervical collision cancer. In all, 9558 patients from 22 studies were included after a systematic data search, performed comprehensively using the following databases: MEDLINE, Web of Science, Embase, and Cochrane. The meta-analysis was conducted with a random-effects model using the Review Manager software (Revman version 5.3). The overall survival (OS), disease-free survival (DFS), and progression-free survival (PFS) data were compared among each observational study. All data are expressed as hazard ratios (HRs) and 95% confidence intervals (CIs), and were calculated using the generic inverse of variance method. Statistical heterogeneity was quantified using Cochrane's Q statistic and Higgins I statistic. Subgroup analysis was performed to investigate the sources of heterogeneity. Furthermore, quality assessment of the included datasets was presented according to the Newcastle-Ottawa Scale method. Additionally, sensitivity analysis was conducted to explore the sources of heterogeneity and analyze whether the results were stable and reliable. Meta-analysis random-effect approach was used for the regression to evaluate the effect of age, presence of squamous cell carcinoma patients, and number of evaluated NLR and PLR parameters on patient survival.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dib.2021.106925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039823PMC
April 2021

Effect of ridge preservation at molar extraction sites with severe periodontitis on the Schneiderian membrane thickness changes: a pilot study.

BMC Oral Health 2021 04 12;21(1):184. Epub 2021 Apr 12.

Department of Restorative Dentistry, University of Washington, Seattle, WA, USA.

Background: Information regarding the reaction of bone augmentation in terms to sinus mucosa thickness of periodontally compromised molar extraction sites is limited. This retrospective study aimed to analyze the effect of ridge preservation procedures following the extraction of molars with severe periodontitis on the healing pattern of adjacent maxillary sinus mucosal membranes.

Methods: Thirty-one periodontally compromised maxillary molar teeth either receiving ridge preservation (test group, n = 20) or undergoing spontaneous healing (control group, n = 11) were investigated. Cone-beam computed tomography (CBCT) scanning was performed before the extraction procedure and repeated 6 months later. The mucosa thickness (MT) of the adjacent periodontally compromised molar tooth was measured from CBCT images before tooth extraction and after 6 months of healing at nine assigned measurement points. The data were analyzed at α = 0.05.

Results: The prevalence of pre-extraction maxillary sinus mucosal thickening was 60.0% and 63.6% in the test and control groups, respectively. The average MT of the thickened sinus mucosa before tooth extraction was 3.78 ± 2.36 mm in the test group and 4.63 ± 3.20 mm in the control group (P = 0.063). The mean mucosal thickening reductions in the thickened MT subjects after 6 months of healing were 2.20 ± 2.05 mm (test group) and 2.64 ± 2.70 mm (control group), P = 0.289. The differences of MT between the time prior to extraction and after 6 months of healing were statistically significant within both groups (P < 0.05).

Conclusions: Following extraction of molars with severe periodontitis, a reduction in swelling of the Schneiderian membrane has been observed regardless of the addition of a DBBM socket graft. However, a mucosal thickness > 2 mm was still frequently observed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12903-021-01546-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040225PMC
April 2021

Causal effects of plasma lipids on the risk of atrial fibrillation: A multivariable mendelian randomization study.

Nutr Metab Cardiovasc Dis 2021 05 20;31(5):1569-1578. Epub 2021 Feb 20.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Background And Aims: Observational studies have suggested that plasma lipids contribute substantially to cardiovascular disease, but "cholesterol paradox" in atrial fibrillation (AF) remains. We sought to investigate the causal effects of lipid profiles on the risk of AF.

Methods And Results: Two-sample Mendelian randomization (MR) framework was implemented to examine the causality of association. Summary estimations of genetic variants associated with low density lipoprotein (LDL)-cholesterol, high density lipoprotein (HDL)-cholesterol, total cholesterol, triglycerides, lipoprotein-a [Lp(a)], apolipoprotein A1 (ApoA 1), and apolipoprotein B (ApoB) were 81, 99, 96, 61, 30, 10, and 23 single nucleotide polymorphisms, respectively. Genetic association with AF were retrieved from a genome-wide association study that included 1,030,836 individuals. The complications for AF were predefined as cardioembolic stroke (CES) and heart failure (HF). In the multivariable MR, the odds ratios for AF per standard deviation (SD) increase were 1.030 (95% confidence interval (CI) 0.979-1.083; P = 0.257) for LDL-cholesterol, 0.986 (95% CI 0.931-1.044; P = 0.622) for HDL-cholesterol, 0.965 (95% CI 0.896-1.041; P = 0.359) for triglycerides, 1.001 (95% CI 1.000-1.003; P = 0.023) for Lp(a), 1.017 (95% CI 0.966-1.070; P = 0.518) for ApoA1, and 1.002 (95% CI 0.963-1.043; P = 0.923) for ApoB. There was no evidence that other lipid components were causally associated with AF, CES, or HF, other than for a marginal association between triglycerides and HF.

Conclusions: This MR study provides robust evidence that high Lp(a) increases the risk of AF, suggesting that interventions targeting Lp(a) may contribute to the primary prevention of AF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.numecd.2021.02.011DOI Listing
May 2021

Acceptability, Appropriateness, and Preliminary Effects of the PrEP Diffusion Training for Lay HIV Workers: Increased PrEP Knowledge, Decreased Stigma, and Diffusion of Innovation.

AIDS Behav 2021 Mar 31. Epub 2021 Mar 31.

Aaron Diamond AIDS Research Center, Columbia University Irving Medical Center, 701 W. 168 St, Hammer Health Sciences Center Building 1108, New York, NY, 10032, USA.

Training lay health workers is a critical intervention strategy to support HIV pre-exposure prophylaxis (PrEP) implementation. However, few evaluations of such trainings have been published. We conducted multi-time-point surveys to evaluate the effect of a training intervention on knowledge, PrEP stigma, and implementation behavior among lay HIV workers in China. Results indicated high acceptability and appropriateness of the training. PrEP knowledge score increased by 65% from pre- to post-training, and remained high one-month post-training. We observed a significant decrease in PrEP stigma and a positive influence on determinants of implementation behaviors. All lay HIV workers surveyed one-month after the training reported having disseminated PrEP information in social networks; 43% reported integrating PrEP education into routine work. The training is an effective strategy to build lay HIV workers' capacity in PrEP implementation. Longer-term post-training follow up would be ideal to further assess actual PrEP uptake and sustained effects on PrEP implementation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10461-021-03248-2DOI Listing
March 2021

Complete laparoscopic radical resection of hilar cholangiocarcinoma: technical aspects and long-term results from a single center.

Wideochir Inne Tech Maloinwazyjne 2021 Mar 16;16(1):62-75. Epub 2020 Jul 16.

Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

Introduction: The landscape of surgical treatments for hepatobiliary disease was significantly changed after the advent of laparoscopy. Many kinds of complex laparoscopic procedures can be routinely performed at present, but radical resection of hilar cholangiocarcinoma (HC) by laparoscopy is still highly contentious.

Aim: To describe our primary experience with laparoscopic radical resection for HC and determine the safety and feasibility of this procedure.

Material And Methods: Between December 2015 and November 2019, 32 patients planned to undergo curative-intent laparoscopic resection of HC in our department. The perioperative and long-term outcomes of these patients were retrospectively analyzed.

Results: Laparoscopic surgery with radical resection was ultimately performed in 24 (75.0%) patients; 3 (9.3%) patients were found to be unresectable at the preliminary exploration stage, and 5 (15.7%) patients converted from laparoscopy to laparotomy. The operation time and blood loss were 476.95 ±133.89 min and 568.75 ±324.01 ml, respectively. A negative margin was achieved in 19 (79.1%) of the laparoscopy patients. Three (12.5%) patients were identified with microscopic positive margins, and 2 (8.4%) patients underwent macroscopic residual tumor resection (R2). The length of postoperative stay was 23.3 ±11.7 days. Severe morbidity occurred in 4 (16.6%) patients. The actuarial 3-year overall survival and disease-free survival for patients who underwent laparoscopic surgery were 49.1% and 47.0%, respectively.

Conclusions: Laparoscopic radical resection for HC is safe and feasible in experienced hands for highly selected patients but is still in its initial stages. When adequate oncologic resection is performed, the laparoscopic approach does not adversely influence the prognosis of the patient.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5114/wiitm.2020.97363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991938PMC
March 2021

Preparation and characterization of gelatin-polysaccharide composite hydrogels for tissue engineering.

PeerJ 2021 15;9:e11022. Epub 2021 Mar 15.

College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan, China.

Background: Tissue engineering, which involves the selection of scaffold materials, presents a new therapeutic strategy for damaged tissues or organs. Scaffold design based on blends of proteins and polysaccharides, as mimicry of the native extracellular matrix, has recently become a valuable strategy for tissue engineering.

Objective: This study aimed to construct composite hydrogels based on natural polymers for tissue engineering.

Methods: Composite hydrogels based on blends of gelatin with a polysaccharide component (chitosan or alginate) were produced and subsequently enzyme crosslinked. The other three hydrogels, chitosan hydrogel, sodium alginate hydrogel, and microbial transglutaminase-crosslinked gelatin (mTG/GA) hydrogel were also prepared. All hydrogels were evaluated for in vitro degradation property, swelling capacity, and mechanical property. Rat adipose-derived stromal stem cells (ADSCs) were isolated and seeded on (or embedded into) the above-mentioned hydrogels. The morphological features of ADSCs were observed and recorded. The effects of the hydrogels on ADSC survival and adhesion were investigated by immunofluorescence staining. Cell proliferation was tested by thiazolyl blue tetrazolium bromide (MTT) assay.

Results: Cell viability assay results showed that the five hydrogels are not cytotoxic. The mTG/GA and its composite hydrogels showed higher compressive moduli than the single-component chitosan and alginate hydrogels. MTT assay results showed that ADSCs proliferated better on the composite hydrogels than on the chitosan and alginate hydrogels. Light microscope observation and cell cytoskeleton staining showed that hydrogel strength had obvious effects on cell growth and adhesion. The ADSCs seeded on chitosan and alginate hydrogels plunged into the hydrogels and could not stretch out due to the low strength of the hydrogel, whereas cells seeded on composite hydrogels with higher elastic modulus, could spread out, and grew in size.

Conclusion: The gelatin-polysaccharide composite hydrogels could serve as attractive biomaterials for tissue engineering due to their easy preparation and favorable biophysical properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.11022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971083PMC
March 2021

Persistent organic pollutants (POPs) in deep-sea sediments of the tropical western Pacific Ocean.

Chemosphere 2021 Aug 15;277:130267. Epub 2021 Mar 15.

MNR Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China. Electronic address:

Persistent organic pollutants (POPs) are toxic compounds that can persist for extended periods in the environment. The marine environment is considered an important sink for POPs. However, information regarding POPs in deep-sea environments remains limited. In this study, surface sediments from depths below 2,000 m were collected in the western Pacific Ocean to analyze polycyclic aromatic hydrocarbons (PAHs), organic pesticides, and polychlorinated biphenyls (PCBs). The concentrations of PAHs were highest (5.2-24.6 ng g dw). Hexachlorocyclohexanes (HCHs) were the predominant organic pesticide (30-1,730 pg g dw). Dicofol, chlorpyrifos, and malathion were detected only at a few sites. PCBs were not detected in the study area. A principal component analysis with multiple linear regression (PCA-MLR) indicated that PAHs in sediments mainly originated from biomass and coal combustion (∼62%) and petrogenic (∼38%) sources. This study revealed the distribution and potential sources of POPs in sediments of a deep-sea region in the western Pacific Ocean. Further studies of the transformations, sedimentation, and biological interactions of POPs are needed to better understand the fates of POPs in the marine environment and the ecological risks they pose.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130267DOI Listing
August 2021

Abnormal upregulation of cardiovascular disease biomarker PLA2G7 induced by proinflammatory macrophages in COVID-19 patients.

Sci Rep 2021 03 24;11(1):6811. Epub 2021 Mar 24.

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

High rate of cardiovascular disease (CVD) has been reported among patients with coronavirus disease 2019 (COVID-19). Importantly, CVD, as one of the comorbidities, could also increase the risks of the severity of COVID-19. Here we identified phospholipase A2 group VII (PLA2G7), a well-studied CVD biomarker, as a hub gene in COVID-19 though an integrated hypothesis-free genomic analysis on nasal swabs (n = 486) from patients with COVID-19. PLA2G7 was further found to be predominantly expressed by proinflammatory macrophages in lungs emerging with progression of COVID-19. In the validation stage, RNA level of PLA2G7 was identified in nasal swabs from both COVID-19 and pneumonia patients, other than health individuals. The positive rate of PLA2G7 were correlated with not only viral loads but also severity of pneumonia in non-COVID-19 patients. Serum protein levels of PLA2G7 were found to be elevated and beyond the normal limit in COVID-19 patients, especially among those re-positive patients. We identified and validated PLA2G7, a biomarker for CVD, was abnormally enhanced in COVID-19 at both nucleotide and protein aspects. These findings provided indications into the prevalence of cardiovascular involvements seen in patients with COVID-19. PLA2G7 could be a potential prognostic and therapeutic target in COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-85848-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990942PMC
March 2021

Coupling Genomics and Hydraulic Information to Predict the Nitrogen Dynamics in a Channel Confluence.

Environ Sci Technol 2021 04 24;55(8):4616-4628. Epub 2021 Mar 24.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, People's Republic of China.

The simulation of nitrogen dynamics in urban channel confluences is essential for the evaluation and improvement of water quality. The omics-based modeling approaches that have been rapidly developed have been increasingly applied to characterize metabolisms of the microbial community and transformation of the associated materials. However, the transport of microorganisms and chemicals within and among different phases, which could be the rate-limiting step for the nitrogen dynamics, are always neglected or oversimplified in omics-based models. Therefore, this study proposes a novel simulation system coupling genomic and hydraulic information to simulate transport and transformation processes and provide predictions of nitrogen dynamics in a confluence. The proposed model was able to capture multiphase mass transport, microbial population dynamics, and nitrogen transformation and accurately predict gene abundances and nitrogen concentrations in both water and sediment; the mean relative errors were all lower than 40%. The model emphasized the importance of transport processes, which contributed more than 90% to gene abundances and chemical concentrations. Moreover, the simulation of reaction rates exhibited the specific nitrogen transformation processes in the confluence. The sulfide oxidation and the nitrate reduction and anaerobic ammonium oxidation, with the participation of the genes and , respectively, were promoted as the main processes of nitrate and nitrite reduction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c04018DOI Listing
April 2021

Clinical evaluations of free gingival grafting before implant placement to increase keratinized tissue width in molar regions: A retrospective case series.

Clin Oral Implants Res 2021 Mar 23. Epub 2021 Mar 23.

Department of Restorative Dentistry, University of Washington, Seattle, WA, USA.

Objectives: To evaluate the dimensional changes of the keratinized tissue width (KTW) in molar regions after augmentation by free gingival grafts (FGG) before implant placement.

Material And Methods: In seventeen patients, twenty implant sites in molar regions with KTW ≤3 mm at the buccal aspect received FGG 2 months before implant placement. KTW at the buccal aspect was measured before FGG (T0), immediately before implant placement (T1), at the time of impression taking for final prosthesis fabrication (T2), and at the end of the follow-up period after loading (T3, 12-48 months). Changes in KTW before and after FGG, as well as the alterations during the follow-up period after loading, were analyzed. Shapiro-Wilk test, paired Student's t test, and Wilcoxon signed-rank test were used for the data analysis at α = 0.05.

Results: KTW at the buccal aspect of the alveolar ridge was observed with a significant gain of 5.9 ± 1.3 mm at T1 (p < .001). The shrinkage of KTW from T2 to T3 was 8.5%, which was limited but statistically significant (p = .008). KTW at the buccal aspect of implant restorations was 5.0 ± 1.5 mm at T3.

Conclusions: Within the limitations of the present study, our data suggest that using FGG to increase KTW in molar regions before implant placement had a predictable result. The buccal KTW had a limited reduction and was ≥3 mm with more than 12 months of follow-up after loading.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/clr.13748DOI Listing
March 2021

3-D hierarchical porous carbon from oxidized lignin by one-step activation for high-performance supercapacitor.

Int J Biol Macromol 2021 Jun 13;180:51-60. Epub 2021 Mar 13.

Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Rural Environment Protection Engineering & Technology Center of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.

To convert lignin into high-valued carbon materials and understand the lignin structure function, oxidized lignin, a by-product from lignocellulose PHP-pretreatment (phosphoric acid plus hydrogen peroxide), was carbonized by one-step KOH-activation; the physico-chemical characteristics and electrochemical performances of the harvested carbons were also investigated. Results indicated the resultant carbons displayed 3-dimensional hierarchical porous morphology with maximum specific surface area of 3094 m g and pore volume of 1.72 cm g using 3:1 KOH/lignin ratio for carbonization. Three-electrode determination achieved a specific capacitance of 352.9 F g at a current of 0.5 A g, suggesting a superior rate performance of this carbon. Two-electrode determination obtained an excellent energy density of 9.5 W h kg at power density of 25.0 W kg. Moreover, 5000 cycles of charge/discharge reached 88.46% retention at 5 A g, implying an outstanding cycle stability. Basically, low molecular weight and abundant oxygen-containing functional groups of employed lignin mainly related to the excellent porous morphology and the outstanding electrochemical performances, suggesting the oxidized lignin was an ideal precursor to facilely prepare activated carbon for high-performance supercapacitor. Overall, this work provides a new path to valorize lignin by-product derived from oxidative pretreatment techniques, which can further promote the integrality of lignocellulose biorefinery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.03.048DOI Listing
June 2021

PLA1A expression as a diagnostic marker of BRAF-mutant metastasis in melanoma cancer.

Sci Rep 2021 Mar 15;11(1):6056. Epub 2021 Mar 15.

Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.

BRAF and NRAS are the most reported mutations associated to melanomagenesis. The lack of accurate diagnostic markers in response to therapeutic treatment in BRAF/NRAS-driven melanomagenesis is one of the main challenges in melanoma personalized therapy. In order to assess the diagnostic value of phosphatidylserine-specific phospholipase A1-alpha (PLA1A), a potent lysophospholipid mediating the production of lysophosphatidylserine, PLA1A mRNA and serum levels were compared in subjects with malignant melanoma (n = 18), primary melanoma (n = 13), and healthy subjects (n = 10). Additionally, the correlation between histopathological subtypes of BRAF/NRAS-mutated melanoma and PLA1A was analyzed. PLA1A expression was significantly increased during melanogenesis and positively correlated to disease severity and histopathological markers of metastatic melanoma. PLA1A mRNA and serum levels were significantly higher in patients with BRAF-mutated melanoma compared to the patients with NRAS-mutated melanoma. Notably, PLA1A can be used as a diagnostic marker for an efficient discrimination between naïve melanoma samples and advanced melanoma samples (sensitivity 91%, specificity 57%, and AUC 0.99), as well as BRAF-mutated melanoma samples (sensitivity 62%, specificity 61%, and AUC 0.75). Our findings suggest that PLA1A can be considered as a potential diagnostic marker for advanced and BRAF-mutated melanoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-85595-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961027PMC
March 2021

[The difficulties and surgical decision analysis of laparoscopic technique in treating complicated hepatolithiasis].

Zhonghua Wai Ke Za Zhi 2021 Apr 1;59(4):279-283. Epub 2021 Apr 1.

Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College; Institute of Hepatobiliary-Pancreatic-Intestinal Diseases, North Sichuan Medical College, Nanchong 637000, China.

To explore the difficulties and surgical decision of laparoscopic technique in patients with complicated hepatolithiasis. The clinical data of 13 patients with complicated hepatolithiasis who underwent laparoscopic hepatectomy at Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College from December 2019 to December 2020 were collected. There were 3 males and 10 females with average age of 50.8 years (range: 14 to 67 years). All patients had upper abdominal pain and a history of cholecystectomy, 4 of them had fever.Seven cases underwent laparoscopic left hemihepatectomy+bile duct exploration, 2 cases underwent laparoscopic right hemihepatectomy+bile duct exploration, 2 cases underwent laparoscopic quadrate hepatectomy (liver 4B+Part 5)+hilar cholangioplasty+bile duct exploration, 2 cases underwent laparoscopic quadrate lobe resection (liver 4B+Part 5)+cholangioplasty+cholangiojejunostomy. All patients were re-examined with abdominal ultrasound and choledochoscope 3 months after operation. The median operation time was 5.2 hours (range: 3.6-6.5 hours), blood loss was 278 ml (range: 120-580 ml). During the operation, 1 case had duodenal bulb injury, and the defect area was about 1.0 cm × 1.2 cm. After timely detection, the defect area was carefully evaluated and trimmed. Absorbable suture was used to suture duodenal bulb, and gastrojejunostomy was performed after repair. One case had small intestinal serosa injury, which was intermittently sutured and embedded with absorbable suture. All the patients recovered smoothly without death. Three months after the operation, 12 patients completed abdominal ultrasound and T-tube sinus choledochoscopy. Residual stones were found in 3 patients, stones were removed in 2 patients by T-tube sinus choledochoscopy 3 months after the operation, 1 patient was still in the recovery period (within 3 months after surgery). No residual stones were found in the remaining 10 patients. The three key laparoscopic techniques in operation of complex hepatolithiasis: adhesiolysis and porta hepatis exposure, laparoscopic hepatectomy, and laparoscopic choledochojejunostomy are very important.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn112139-20210101-00001DOI Listing
April 2021

Iron and copper micronutrients influences cadmium accumulation in rice grains by altering its transport and allocation.

Sci Total Environ 2021 Jul 2;777:146118. Epub 2021 Mar 2.

School of Environment and Resource, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.

Cadmium (Cd) contamination in rice paddy fields constitutes a serious health issue in some parts of China. Here we study the potential for remediation of Cd contaminated alkaline paddy soil with low iron (Fe) and high copper (Cu) background by altering the concentrations of Fe and Cu in the growing media, which has been only seldom considered. We assessed how these two micronutrients (Cu and Fe) affect the absorption and transport of Cd in rice. Adding Cu significantly increased rice biomass and grain yield by reducing root Cd influx and Cd upward transport which, consequently, lowered Cd concentrations in roots, culms and leaves. However, excessive Cu also promoted a relatively higher Cd allocation in grains, especially under Fe deficiency, likely because Cu significantly increased the proportion of bioavailable Cd in leaves. Contrastingly, Fe did not alleviate the toxic effects of Cd on rice growth and yield, but it significantly reduced Cd transfer towards grains, which might be attributed to a sharp decrease in the proportion of bioavailable Cd in leaves. Our results demonstrated that Cd remediation may be achieved through altering Fe and Cu inputs, such that Cd accumulation in rice grains is reduced.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.146118DOI Listing
July 2021

Microscopic understanding about adsorption and transport of different Cr(VI) species at mineral interfaces.

J Hazard Mater 2021 07 25;414:125485. Epub 2021 Feb 25.

College of Resources and Environments & Chongqing Key Laboratory of Soil Multi-scale Interfacial Process, Southwest University, Chongqing 400715, China. Electronic address:

Cr(VI) ranks as one of the most toxic heavy metals and herein, microscopic mechanisms for adsorption and transport of different Cr(VI) oxyanions (CrO and CrO) at kaolinite interfaces are addressed by dispersion-corrected periodic density functional theory calculations. Cr(VI) oxyanions adsorb favorably at both tetrahedral and octahedral surfaces, and K ions serve as bridge for Cr(VI) oxyanions and tetrahedral surfaces while Cr(VI) oxyanions serve as bridge for K ions and octahedral surfaces. Adsorption structures are altered significantly by pH variation, and stability trends at different pH ranges are deciphered by the dominant interaction force with clay surfaces: Electrostatic interaction with K ions at tetrahedral surfaces whereas combined action of electrostatic and H-bonding interactions with Cr(VI) oxyanions at octahedral surfaces. Electron transfers are strongly pH-dependent, and clay surfaces serve as electron reservoirs. CrO rather than CrO is dominant at clay interfaces, and HCrO can co-exist under acidic conditions. CrO transformation to CrO is kinetically blocked at pH ≈ PZC while preferred at pH < PZC. Cr(VI) removal and reclamation should proceed at pH > 7.0 and pH < PZC, respectively. Results greatly promote the understanding about Cr(VI) bioavailability and fate in surficial environments and are also useful for Cr(VI) removal and reclamation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125485DOI Listing
July 2021

Integrative Genomic Analysis of Gemcitabine Resistance in Pancreatic Cancer by Patient-derived Xenograft Models.

Clin Cancer Res 2021 Jun 5;27(12):3383-3396. Epub 2021 Mar 5.

MOE Key Laboratory of Bioinformatics, Division of BNRist Bioinformatics, Department of Automation, Tsinghua University, Beijing, P.R. China.

Purpose: Gemcitabine is most commonly used for pancreatic cancer. However, the molecular features and mechanisms of the frequently occurring resistance remain unclear. This work aims at exploring the molecular features of gemcitabine resistance and identifying candidate biomarkers and combinatorial targets for the treatment.

Experimental Design: In this study, we established 66 patient-derived xenografts (PDXs) on the basis of clinical pancreatic cancer specimens and treated them with gemcitabine. We generated multiomics data (including whole-exome sequencing, RNA sequencing, miRNA sequencing, and DNA methylation array) of 15 drug-sensitive and 13 -resistant PDXs before and after the gemcitabine treatment. We performed integrative computational analysis to identify the molecular networks related to gemcitabine intrinsic and acquired resistance. Then, short hairpin RNA-based high-content screening was implemented to validate the function of the deregulated genes.

Results: The comprehensive multiomics analysis and functional experiment revealed that and had strong effects on cell proliferation, and and contributed to gemcitabine resistance in pancreatic cancer cells. Moreover, we found miR-135a-5p was significantly associated with the prognosis of patients with pancreatic cancer and could be a candidate biomarker to predict gemcitabine response. Comparing the molecular features before and after the treatment, we found that PI3K-Akt, p53, and hypoxia-inducible factor-1 pathways were significantly altered in multiple patients, providing candidate target pathways for reducing the acquired resistance.

Conclusions: This integrative genomic study systematically investigated the predictive markers and molecular mechanisms of chemoresistance in pancreatic cancer and provides potential therapy targets for overcoming gemcitabine resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-19-3975DOI Listing
June 2021

Suppression of the HBP Function Increases Pancreatic Cancer Cell Sensitivity to a Pan-RAS Inhibitor.

Cells 2021 Feb 18;10(2). Epub 2021 Feb 18.

Department of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milan, Italy.

Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer-related death and the search for a resolutive therapy is still a challenge. Since KRAS is commonly mutated in PDAC and is one of the main drivers of PDAC progression, its inhibition should be a key strategy for treatment, especially considering the recent development of specific KRAS inhibitors. Nevertheless, the effects of KRAS inhibition can be increased through the co-inhibition of other nodes important for cancer development. One of them could be the hexosamine biosynthetic pathway (HBP), whose enhancement is considered fundamental for PDAC. Here, we demonstrate that PDAC cells expressing oncogenic KRAS, owing to an increase in the HBP flux, become strongly reliant on HBP for both proliferation and survival. In particular, upon treatment with two different compounds, 2-deoxyglucose and FR054, inhibiting both HBP and protein -glycosylation, these cells undergo apoptosis significantly more than PDAC cells expressing wild-type KRAS. Importantly, we also show that the combined treatment between FR054 and the pan-RAS inhibitor BI-2852 has an additive negative effect on cell proliferation and survival by means of the suppression of both Akt activity and cyclin D1 expression. Thus, co-inhibition of HBP and oncogenic RAS may represent a novel therapy for PDAC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells10020431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923121PMC
February 2021

The Role of Mitochondria in the Chemoresistance of Pancreatic Cancer Cells.

Cells 2021 Feb 25;10(3). Epub 2021 Feb 25.

General Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.

The first-line chemotherapies for patients with unresectable pancreatic cancer (PC) are 5-fluorouracil (5-FU) and gemcitabine therapy. However, due to chemoresistance the prognosis of patients with PC has not been significantly improved. Mitochondria are essential organelles in eukaryotes that evolved from aerobic bacteria. In recent years, many studies have shown that mitochondria play important roles in tumorigenesis and may act as chemotherapeutic targets in PC. In addition, according to recent studies, mitochondria may play important roles in the chemoresistance of PC by affecting apoptosis, metabolism, mtDNA metabolism, and mitochondrial dynamics. Interfering with some of these factors in mitochondria may improve the sensitivity of PC cells to chemotherapeutic agents, such as gemcitabine, making mitochondria promising targets for overcoming chemoresistance in PC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells10030497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996512PMC
February 2021