Publications by authors named "Gang Liu"

3,777 Publications

  • Page 1 of 1

Prediction of Postoperative Deterioration in Cardiac Surgery Patients using Electronic Health Record and Physiologic Waveform Data.

Anesthesiology 2022 Aug 11. Epub 2022 Aug 11.

Department of Computational Medicine and Bioinformatics, University of Michigan Health System, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.

Background: Postoperative hemodynamic deterioration among cardiac surgical patients can indicate or lead to adverse outcomes. Whereas prediction models for such events using electronic health records or physiologic waveform data are previously described, their combined value remains incompletely defined. The authors hypothesized that models incorporating electronic health record and processed waveform signal data (electrocardiogram lead II, pulse plethysmography, arterial catheter tracing) would yield improved performance versus either modality alone.

Methods: Intensive care unit data were reviewed following elective adult cardiac surgical procedures at an academic center between 2013-2020. Model features included electronic health record features and physiologic waveforms. Tensor decomposition was used for waveform feature reduction. Machine learning-based prediction models included a 2013-2017 training set and a 2017-2020 temporal holdout test set. The primary outcome was a postoperative deterioration event, defined as a composite of low cardiac index <2.0mL/min/m2, mean arterial pressure <55mmHg sustained ≥120 minutes, new or escalated inotrope/vasopressor infusion, epinephrine bolus ≥1mg, or intensive care unit mortality. Prediction models analyzed data 8 hours prior to events.

Results: Among 1,555 cases, 185 (12%) experienced 276 deterioration events, most commonly including low cardiac index (7.0% of patients), new inotrope (1.9%), and sustained hypotension (1.4%). The best performing model on the 2013-2017 training set yielded a c-statistic of 0.803 (95% CI 0.799-0.807) although performance was substantially lower in the 2017-2020 test set (0.709, 0.705-0.712). Test set performance of the combined model was greater than corresponding models limited to solely electronic health record features (0.641, 0.637-0.646) or waveform features (0.697, 0.693-0.701).

Conclusions: Clinical deterioration prediction models combining electronic health record data and waveform data were superior to either modality alone, and performance of combined models was primarily driven by waveform data. Decreased performance of prediction models during temporal validation may be explained by dataset shift, a core challenge of healthcare prediction modeling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/ALN.0000000000004345DOI Listing
August 2022

Uncovering Brain Differences in Preschoolers and Young Adolescents with Autism Spectrum Disorder Using Deep Learning.

Int J Neural Syst 2022 Aug 9:2250044. Epub 2022 Aug 9.

Department of Radiology, First Medical Center, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing, P. R. China.

Identifying brain abnormalities in autism spectrum disorder (ASD) is critical for early diagnosis and intervention. To explore brain differences in ASD and typical development (TD) individuals by detecting structural features using T1-weighted magnetic resonance imaging (MRI), we developed a deep learning-based approach, three-dimensional (3D)-ResNet with inception (I-ResNet), to identify participants with ASD and TD and propose a gradient-based backtracking method to pinpoint image areas that I-ResNet uses more heavily for classification. The proposed method was implemented in a preschool dataset with 110 participants and a public autism brain imaging data exchange (ABIDE) dataset with 1099 participants. An extra epilepsy dataset with 200 participants with clear degeneration in the parahippocampal area was applied as a verification and an extension. Among the datasets, we detected nine brain areas that differed significantly between ASD and TD. From the ROC in PASD and ABIDE, the sensitivity was 0.88 and 0.86, specificity was 0.75 and 0.62, and area under the curve was 0.787 and 0.856. In a word, I-ResNet with gradient-based backtracking could identify brain differences between ASD and TD. This study provides an alternative computer-aided technique for helping physicians to diagnose and screen children with an potential risk of ASD with deep learning model.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1142/S0129065722500447DOI Listing
August 2022

[Retracted] MicroRNA‑655 attenuates the malignant biological behaviours of retinoblastoma cells by directly targeting PAX6 and suppressing the ERK and p38 MAPK signalling pathways.

Oncol Rep 2022 Oct 10;48(4). Epub 2022 Aug 10.

Department of Ophthalmology, Xiangyang No. 1 People's Hospital Affiliated to Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that data shown in various of the panels portraying the results from flow cytometric experiments in Fig. 2D, 5D and 6D were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [Oncology Reports 39: 2040‑2050, 2018; DOI: 10.3892/or.2018.6264].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/or.2022.8385DOI Listing
October 2022

Targeting mucus barrier in respiratory diseases by chemically modified advanced delivery systems.

Chem Biol Interact 2022 Aug 3:110048. Epub 2022 Aug 3.

Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, NSW, 2007, Australia; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, Australia. Electronic address:

Mucus gel constitutes of heavily cross-linked mucin fibers forming a viscoelastic, dense porous network that coats all the exposed epithelia not covered with the skin. The layer provides protection to the underlying gastrointestinal, respiratory, and female reproductive tracts, in addition to the organs such as the surface of eye by trapping the pathogens, irritants, environmental fine particles, and potentially hazardous foreign matter. However, this property of mucus gel poses a substantial challenge for realizing the localized and sustained drug delivery across the mucosal surfaces. The mucus permeating particles that spare the protective properties of mucus gel improve the therapeutic potency of the drugs aimed at the management of diseases, including sexually transmitted infections, lung cancer, irritable bowel disease, degenerative eye diseases and infections, and cystic fibrosis. As such, the mucoadhesive materials conjugated with drug molecules display a prolonged retention time in the mucosal gel that imparts a sustained release of the deliberated drug molecules across the mucosa. The contemporarily developed mucus penetrating materials for drug delivery applications comprise of a finer size, appreciable hydrophilicity, and a neutral surface to escape the entrapment within the cross-inked mucus fibers. Pertaining to the mucus secretion as a first line of defence in respiratory tract in response to the invading physical, chemical, and biological pathogens, the development of mucus penetrating materials hold promise as a stalwart approach for revolutionizing the respiratory drug delivery paradigm. The present review provides an epigrammatic collation of the mucus penetrating/mucoadhesive materials for achieving a controlled/sustained release of the cargo pharmaceutics and drug molecules across the respiratory mucus barrier.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbi.2022.110048DOI Listing
August 2022

A Highly Selective Hg Fluorescent Chemosensor Based On Photochromic Diarylethene With Quinoline Unit.

J Fluoresc 2022 Aug 6. Epub 2022 Aug 6.

Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, People's Republic of China.

A novel diarylethene-based fluorescent chemosensor containing a quinoline unit (1o) had been designed and synthesized. 1o showed good photochromic ability and fluorescence switching properties by alternating UV/vis light irradiation. The chemosensor showed high "Turn-off" fluorescent selectivity for Hg by competitive tests of the fluorescence reaction in the presence other ions in acetonitrile solution. The stoichiometry between the compound 1o and Hg was 1:1 by Job's plot curve and HRMS analysis. In addition, the LOD for Hg was calculated as 60 nM. The fluorescence emission can be back to the "Turn-on" state by adding EDTA. Based on these facts, a molecular logic gate that including four input signals (UV/vis and Hg/EDTA) and one output signal (fluorescent intensity at 491 nm) was designed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10895-022-02930-5DOI Listing
August 2022

Reactive oxygen species-responsive and Raman-traceable hydrogel combining photodynamic and immune therapy for postsurgical cancer treatment.

Nat Commun 2022 Aug 5;13(1):4553. Epub 2022 Aug 5.

National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.

Combining immune checkpoint blockade (ICB) therapy with photodynamic therapy (PDT) holds great potential in treating immunologically "cold" tumors, but photo-generated reactive oxygen species (ROS) can inevitably damage co-administered ICB antibodies, hence hampering the therapeutic outcome. Here we create a ROS-responsive hydrogel to realize the sustained co-delivery of photosensitizers and ICB antibodies. During PDT, the hydrogel skeleton poly(deca-4,6-diynedioic acid) (PDDA) protects ICB antibodies by scavenging the harmful ROS, and at the same time, triggers the gradual degradation of the hydrogel to release the drugs in a controlled manner. More interestingly, we can visualize the ROS-responsive hydrogel degradation by Raman imaging, given the ultrastrong and degradation-correlative Raman signal of PDDA in the cellular silent window. A single administration of the hydrogel not only completely inhibits the long-term postoperative recurrence and metastasis of 4T1-tumor-bearing mice, but also effectively restrains the growth of re-challenged tumors. The PDDA-based ROS-responsive hydrogel herein paves a promising way for the durable synergy of PDT and ICB therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-32160-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356008PMC
August 2022

Topological Gradients for Metal Film-Based Strain Sensors.

Nano Lett 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Metal film-based stretchable strain sensors hold great promise for applications in various domains, which require superior sensitivity-stretchability-cyclic stability synergy. However, the sensitivity-stretchability trade-off has been a long-standing dilemma and the metal film-based strain sensors usually suffer from weak cyclic durability, both of which significantly limit their practical applications. Here, we propose an extremely facile, low-cost and spontaneous strategy that incorporates topological gradients in metal film-based strain sensors, composed of intrinsic (grain size and interface) and extrinsic (film thickness and wrinkle) microstructures. The topological gradient strain sensor exhibits an ultrawide stretchability of 100% while simultaneously maintaining a high sensitivity at an optimal topological gradient of 4.5, due to the topological gradients-induced multistage film cracking. Additionally, it possesses a decent cyclic stability for >10 000 cycles between 0 and 40% strain enabled by the gradient-mixed metal/elastomer interfaces. It can monitor the full-range human activities from subtle pulse signals to vigorous joint movements.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.2c01967DOI Listing
August 2022

Sustained-Release Ivabradine Hemisulfate in Patients With Systolic Heart Failure.

J Am Coll Cardiol 2022 Aug;80(6):584-594

Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Background: Ivabradine has potent actions in reducing heart rate and improving clinical outcomes of chronic heart failure with reduced ejection fraction (HFrEF). At present, only the short-acting formulation of ivabradine is available that needs to be administered twice daily.

Objectives: This study sought to evaluate the role of ivabradine hemisulfate sustained release (SR), a novel long-acting formulation of ivabradine dosed once daily, in stable patients with HFrEF.

Methods: Patients with stabilized HFrEF in New York Heart Association functional class II-IV were enrolled and randomized to receive placebo or ivabradine SR in addition to standard medications. The primary endpoint was the change of left ventricular (LV) end-systolic volume index from baseline to week 32.

Results: We randomly assigned 181 patients to placebo and 179 patients to ivabradine SR. After 32 weeks, a significant improvement of LV end-systolic volume index from baseline was observed in both arms with a greater effect in the ivabradine SR arm. Ivabradine SR therapy also exhibited superiority in improving LV end-diastolic volume index, LV ejection fraction, resting heart rate, the Kansas City Cardiomyopathy Questionnaire score, and hospital admission for heart failure worsening and cardiovascular disease in comparison to placebo. Overall adverse events showed no difference between the treatment arms. There were fewer occurrences of worsening heart failure in the ivabradine SR arm.

Conclusions: The present study demonstrates that ivabradine SR once daily in addition to optimum standard therapy improved heart function in patients with HFrEF. (Clinical Trial of Systolic Heart Failure Treatment of IvabRadine Hemisulfate Sustained-release Tablets [FIRST]; NCT02188082).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jacc.2022.05.027DOI Listing
August 2022

Different Lengths of Percutaneous Transverse Iliosacral Screw in Geometric Osseous Fixation Pathway: A Finite-Element Analysis.

Indian J Orthop 2022 Aug 1;56(8):1354-1362. Epub 2022 Jun 1.

Department of Orthopaedics, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, People's Republic of China.

Objective: To evaluate the biomechanical performances of the sacroiliac screw fixation of the first sacral vertebra with different lengths of screws using the Finite-Element Method.

Methods: First, pelvic CT images were generated from a healthy volunteer, and multislice sagittal views were produced to determine the axis for the first sacral vertebra geometric osseous fixation pathway (GOFP). Subsequently, according to the geometric size and mechanical parameters of the iliosacral screw, the screw models with the same diameter of 7.3 mm and different lengths of 80 mm, 90 mm, 100 mm, 110 mm, 120 mm, 130 mm and 140 mm were built. Then the seven screws were assembled with the pelvic model. The maximum von Mises stress and the shape variables were evaluated for the pelvis and the screws.

Results: Results are shown for the pelvic and GOFP screw, respectively. The simulation results show that the maximum von Mises stress in the cortex of the pelvic ring of the pelvis with the 130-mm length screw is the lowest among the pelvic models with different screws. Moreover, the peak displacement of the pelvis with the 130-mm length screw is the smallest. These results indicate that under the standing condition, a 130-mm length screw can decrease the stress concentration and result in a more effective transfer of stress within the reconstructed pelvis. In addition, the displacement of the screw with a 130-mm length is the smallest among all the considered screws. The peak von Mises stresses in the 130-mm length screw and the cortex are still within a low and elastic range below the yielding strengths of the materials.

Conclusion: Through the finite element analysis, the GOFP can be used as a safe and effective way for iliosacral screw fixation. The optimal length of the screw may be 130 mm length.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43465-022-00656-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283617PMC
August 2022

Tracking Three Decades of Global Neodymium Stocks and Flows with a Trade-Linked Multiregional Material Flow Analysis.

Environ Sci Technol 2022 Aug 3. Epub 2022 Aug 3.

SDU Life Cycle Engineering, Department of Green Technology, Faculty of Engineering, University of Southern Denmark, 5230 Odense, Denmark.

Neodymium (Nd), an essential type of rare earth element, has attracted increasing attention in recent years due to its significant role in emerging technologies and its globally imbalanced demand and supply. Understanding the global and regional Nd stocks and flows would thus be important for understanding and mitigating potential supply risks. In this work, we applied a trade-linked multiregional material flow analysis to map the global and regional neodymium cycles from 1990 to 2020. We reveal increasingly complex trade patterns of Nd-containing products and a clearly dominant but slightly weakening role of China in the global Nd trade (for both raw materials and semi- and final products) along the life cycle in the last 30 years. A total of 880 kt Nd was mined accumulatively and flowed into the global socioeconomic system, mainly as NdFeB permanent magnets (79%) in semi-products and conventional vehicles and home appliances (together 48%) in final products. Approximately 64% (i.e., 563 kt Nd) of all the mined Nd globally were not recycled, indicating a largely untapped potential of recycling in securing Nd supply and an urgency to overcome the present technological and non-technical challenges. The global Nd cycle in the past three decades is characterized by different but complementary roles of different regions along the global Nd value chain: China dominates in the provision of raw materials and semi- and final products, Japan focuses on the manufacturing of magnets and electronics, and the United States and European Union show advantages in the vehicle industry. Anticipating increasing demand of Nd in emerging energy and transport technologies in the future, more coordinated efforts among different regions and increased recycling are urgently needed for ensuring both regional and global Nd supply and demand balance and a common green future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.2c02247DOI Listing
August 2022

Novel mutation in TTC21A triggers partial nonsense-mediated mRNA decay and causes male infertility with MMAF.

Clin Genet 2022 Aug 3. Epub 2022 Aug 3.

Department of Andrology, Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cge.14197DOI Listing
August 2022

A highly selective colorimetric and fluorescent probe Eu(tdl)abp for HS sensing: Application in live cell imaging and natural water.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 21;282:121657. Epub 2022 Jul 21.

Jiangxi Key Laboratory of Organic Chemistry, College of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330031, PR China; Department of Ecology and Environment, Yuzhang Normal University, Nanchang 330103, PR China. Electronic address:

Using 4-([2,2': 6', 2'- terpyridin] -4'-yl) -N, N-dimethylaniline (tdl) as auxiliary ligand and 6-azido-2,2'-bipyridine (abp) as recognition ligand, a europium complex fluorescent probe Eu(4-([2,2': 6', 2'-terpyridin] -4' -yl) -N, N-dimethylaniline)-6-azido-2,2'-bipyridine Eu(tdl)abp for efficient and specific recognition of hydrogen sulfide (HS) was successfully synthesized and characterized by NMR and MS. Eu(tdl)abp represented "on-off" fluorescence signals for HS and its color changes could be identified with naked eyes. Eu(tdl)abp had short response time (2 min) to HS, high selectivity and good anti-interference, large stokes shift (207 nm). In various samples, when HS existed, the azide group was reduced to amine group, resulting in closed fluorescence signal, and the fluorescence intensity reached the degree of quenching without being affected by other interference. At the same time, there was a good linear relationship between relative fluorescence intensity and HS concentration with the detection limit (LOD) of 0.64 μM. The sensing mechanism of Eu(tdl)abp to detect HS was characterized by H NMR and HR-MS. Eu(tdl)abp was used with success for the sensitive detection of HS in natural water and living cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2022.121657DOI Listing
July 2022

Case Report: An Unusual Case of Pulmonary Metastatic Adenocarcinoma From Low-Grade Appendiceal Mucinous Neoplasms.

Front Oncol 2022 13;12:906344. Epub 2022 Jul 13.

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.

Background: Low-grade appendiceal mucinous neoplasms (LAMNs) are indolent tumors with low-grade cytology. Although peritoneal dissemination is common due to tumor rupture and mucinous deposits on the visceral peritoneal surface, distant involvement, such as lung, is rarely seen due to lack of invasiveness.

Case Presentation: A 70-year-old woman presented to the hospital due to continuously elevated carcinoembryonic antigen (CEA) levels for 10 months without any symptoms. PET/CT revealed two lesions located in the left lung and appendix. The postoperative pathology results revealed pulmonary mucinous adenocarcinoma and LAMN. Then we performed next-generation sequencing (NGS) to clarify the relationship between the two tumors. The sequencing result showed that both tumors harbored the common tumor mutations, KRAS (p.G12D), GNAS (p.R201H), and BRAF (p.R735Q), which indicated that the pulmonary tumor was a metastasis of LAMN.

Conclusion: This case is unusual in that the primary LAMN and the pulmonary metastasis are present at the time of diagnosis. This study reported the first pulmonary metastasis from LAMN verified by NGS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.906344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327614PMC
July 2022

Analysis of the Quantitative Evaluation of the Public Medical and Health System Costs During Pandemic Governance: Investigation Based on COVID-19.

Front Public Health 2022 13;10:942043. Epub 2022 Jul 13.

School of Tourism, Hainan University, Haikou, China.

It is of great reference significance for broadening the research perspective of pandemic governance, improving the efficiency of pandemic governance and the credibility of the government, to scientifically measure and analyze the public medical and health system costs. This article takes the typical case "pandemic prevention and control event of S city, China" as the research background. First, the concept of public medical and health system costs during pandemic governance is defined. Then, the public medical and health system costs are embedded into the pandemic governance system, and the generation process of the public medical and health system costs in the actual situation are investigated. Furthermore, through in-depth interview, multi-case grounded theory and fuzzy subordinate function analysis, the scientific construction of the public medical and health system cost index system are completed. Finally, based on G1 method/entropy method combined with weighting and fuzzy comprehensive evaluation method, the public medical and health system costs of the pandemic prevention and control events of S city is measured. The results show the following: (1) it is important that good single dimensions and reliable indicators are embodied in the public medical and health system costs scale. Among them, the behavioral public medical and health system costs of the masses is the largest proportion of all indicators; (2) after the pandemic prevention and control event is over, the public medical and health system cost are difficult to repair, and some lagging ideas and behaviors shown by local governments lead to a continuous expansion of the public medical and health system costs associated with pandemic governance; and (3) local governments should not conceal information asymmetry. Instead, local governments should give greater freedom to other actors to deal with pandemic governance, and governance entities should cooperate with each other. This will mitigate the effect of public medical and health system costs. Corresponding policy recommendations are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.942043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326215PMC
August 2022

Case Report: First Case of Endophthalmitis Caused by an Emerging Pathogen: .

Front Public Health 2022 14;10:933851. Epub 2022 Jul 14.

The Department of Ophthalmology, Beijing Children's Hospital Affiliated With Capital Medical University, Beijing, China.

endophthalmitis is a relatively uncommon form of endophthalmitis seen in clinical patients. In general, endophthalmitis tends to carry a poor prognosis. Here, we report a 3-year-old child who was admitted to the hospital due to a rupture of the left eye. The suturing and anterior chamber formation were performed immediately. Approximately, 16 days after the operation, massive whitish plump and tufted exudates gathered in the pupil area and at the bottom of the anterior chamber, and the child was diagnosed with endophthalmitis. The infection was initially considered to be caused by fungal pathogens for that the hyphae and spores were observed in the smear. However, the isolate obtained after 4 days of culturation was identified as actinomycetes using MALDI-TOF. We further classified it as by next-generation sequencing (NGS) based on the MinION platform. Amikacin and sulfamethoxazole tablets were used to control the infection and the ocular inflammation subsided gradually. Intraocular lens (IOL) implantation is planned to be performed at an appropriate future time to improve his vision. endophthalmitis is rare and usually caused by ocular trauma or surgery. In conclusion, should be considered as an emerging pathogen and deserves more attention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.933851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330429PMC
August 2022

Clinical analysis of Wernicke encephalopathy after liver transplantation.

Hepatobiliary Pancreat Dis Int 2022 Jul 21. Epub 2022 Jul 21.

Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; NHFPC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou 310003, China; Key Laboratory of the diagnosis and treatment of organ Transplantation, Chinese Academy of Medical Sciences, Hangzhou 310003, China; Key Laboratory of Organ Transplantation, Research Center for Diagnosis and Treatment of Hepatobiliary Diseases, Zhejiang Province, Hangzhou 310003, China; Collaborative Innovation Center for Diagnosis Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address:

Background: Wernicke encephalopathy (WE) is an acute neurological disease resulting from vitamin B1 deficiency, and there are only very few case reports of WE after liver transplantation. The present study aimed to investigate the clinical characteristics, etiology, magnetic resonance imaging (MRI) features, treatment and prognosis of patients with WE after liver transplantation.

Methods: Twenty-three patients with WE after liver transplantation from the First Affiliated Hospital, Zhejiang University School of Medicine and Jiangxi Provincial People's Hospital between January 2011 and December 2021 were retrospectively analyzed.

Results: Among the 23 patients diagnosed with WE after liver transplantation, 6 (26%) had a classic triad of impaired consciousness, oculomotor palsy and ataxia, 17 (74%) had two features. The misdiagnosis rate was 65%. After treatment with high-dose vitamin B1, 19 (83%) patients showed improvement, whereas 4 showed no improvement, including 3 with residual short-term memory impairments and 1 with residual spatial and temporal disorientation and ataxia.

Conclusions: The misdiagnosis rate is high in the early stage of WE, and the prognosis is closely associated with whether WE is diagnosed early and treated timely. High-dose glucose or glucocorticoids can trigger WE and cannot be administered before vitamin B1 treatment. Vitamin B1 is suggested to be used as a prophylactic treatment for patients with WE after liver transplantation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.hbpd.2022.07.005DOI Listing
July 2022

Activation of GPR81 Aggravates Remote Organ Injury During Hepatic Ischemia-Reperfusion Injury.

Transplant Proc 2022 Jul 25. Epub 2022 Jul 25.

Department of Pathophysiology, Basic Medical College, Chongqing Medical University, Chongqing, China; Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China. Electronic address:

Hepatic ischemia-reperfusion injury (HIRI) is a serious situation with high morbidity and mortality, which is usually accompanied with hyperlactatemia due to impaired lactate clearance in liver. G-protein-coupled receptor 81 (GPR81) has recently been identified as the bioactive receptor of lactate. GPR81 is profoundly involved in the modulation of metabolism and inflammation, but its significance in HIRI remains unclear. The present study investigated the potential roles of GPR81 in HIRI by using the GPR81 agonist 3-chloro-5-hydroxybenzoic acid (CHBA). The results indicated that treatment with CHBA had no obvious effects on HIRI-induced histologic abnormalities and elevation of serum aspartate aminotransferase, alanine aminotransferase. However, CHBA significantly upregulated the serum level of tumor necrosis factor alpha and interleukin-6 in mice with HIRI. Administration of CHBA also exacerbated HIRI-induced histologic lesions in lung, increased the level of myeloperoxidase in lung tissue and the protein concentration in bronchoalveolar lavage fluid. In addition, the serum levels of brain natriuretic peptide and creatinine also increased in CHBA-treated mice. The results indicate that activation of GPR81 might aggravate HIRI-induced remote organ injury and result in serious outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.transproceed.2022.04.024DOI Listing
July 2022

Correlation Between Serum β2-GPI/oxLDL and the Risk of Cerebral Infarction in Patients with T2DM.

Front Surg 2022 11;9:930701. Epub 2022 Jul 11.

The Second Affiliated Hospital, Department of GCP, Hengyang Medical School, University of South China, Hengyang, China.

Objective: This study aims to study the correlation between serum β2-glycoprotein I (β2-GPI)/oxidized low-density lipoprotein (oxLDL) and the risk of cerebral infarction in patients with type 2 diabetes (T2DM).

Methods: From January 2019 to March 2021, 56 patients with T2DM combined with cerebral infarction were chosen as a diabetic cerebral infarction (DCI) group, and 60 patients with simple T2DM were chosen as a T2DM group. In addition, 60 healthy volunteers were recruited as a control group. The essential information of each group was collected, and the serum β2-GPI/oxLDL and inflammatory factor levels in each group were compared. The clinical factors that affect the risk of ischemic cerebral infarction in patients with T2DM were analyzed by a logistic model.

Results: Compared with the control group, the level of serum β2-GPI/oxLDL in the T2DM and DCI groups increased significantly, < 0.001. Compared with the T2DM group, the serum β2-GPI/oxLDL level in the DCI group increased significantly, < 0.05. The result of correlation analysis showed that serum β2-GPI/oxLDL was positively correlated with total cholesterol, triglycerides, fasting blood glucose, 2-h postprandial blood glucose, glycosylated hemoglobin, interleukin-6, and tumor necrosis factor (TNF)-α (all 's< 0.05). Serum TNF-α and β2-GPI/oxLDL were independent risk variates for DCI (< 0.05). Based on the receiver operating characteristic curve analysis, the values of the area under the curve for TNF-α, serum β2-GPI/oxLDL, and the combined diagnosis of DCI were 0.653 (0.552-0.753), 0.680 (0.583-0.777), 0.739 (0.647-0.831), respectively.

Conclusion: In DCI patients, the levels of serum oxLDL/β2-GPI are significantly increased. Serum oxLDL/β2-GPI is an independent risk factor that affects the occurrence of DCI. In addition, the serum β2-GPI/oxLDL level implicates the lipid metabolism and inflammatory status of the internal environment of DCI patients to a certain extent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fsurg.2022.930701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309478PMC
July 2022

Critical Quantum Metrology in the Non-Linear Quantum Rabi Model.

Entropy (Basel) 2022 Jul 22;24(8). Epub 2022 Jul 22.

EP VI and Center for Electronic Correlations and Magnetism, University of Augsburg, 86135 Augsburg, Germany.

The quantum Rabi model (QRM) with linear coupling between light mode and qubit exhibits the analog of a second-order phase transition for vanishing mode frequency which allows for criticality-enhanced quantum metrology in a few-body system. We show that the QRM including a nonlinear coupling term exhibits much higher measurement precisions due to its first-order-like phase transition at frequency, avoiding the detrimental slowing-down effect close to the critical point of the linear QRM. When a bias term is added to the Hamiltonian, the system can be used as a fluxmeter or magnetometer if implemented in circuit QED platforms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/e24081015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330817PMC
July 2022

Brain Metabolic Connectivity Patterns in Patients with Prolonged Disorder of Consciousness after Hypoxic-Ischemic Injury: A Preliminary Study.

Brain Sci 2022 Jul 7;12(7). Epub 2022 Jul 7.

Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.

Understanding the patterns of brain glucose metabolism and connectivity in hypoxic-ischemic encephalopathy (HIE) patients with prolonged disorders of consciousness (DOC) may be of positive significance to the accurate assessment of consciousness and the optimization of neuromodulation strategy. We retrospectively analyzed the brain glucose metabolism pattern and its correlation with clinical Coma Recovery Scale-Revised (CRS-R) score in six HIE patients with prolonged DOC who had undergone F-deoxyglucose brain positron emission tomography scanning (FDG-PET). We also compared the differences in global metabolic connectivity patterns and the characteristics of several brain networks between HIE patients and healthy controls (HC). The metabolism of multiple brain regions decreased significantly in HIE patients, and the degree of local metabolic preservation was correlated with CRS-R score. The internal metabolic connectivity of occipital lobe and limbic system in HIE patients decreased, and their metabolic connectivity with frontal lobe, parietal lobe and temporal lobe also decreased. The metabolic connectivity patterns of default mode network, dorsal attention network, salience network, executive control network and subcortex network of HIE also changed compared with HC. The present study suggested that pattern of cerebral glucose metabolism and network connectivity of HIE patients with prolonged DOC were significantly different from those of healthy people.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/brainsci12070892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313214PMC
July 2022

Adaptability and Evolution of Gobiidae: A Genetic Exploration.

Animals (Basel) 2022 Jul 6;12(14). Epub 2022 Jul 6.

College of Life Sciences, Qufu Normal University, Jingxuan West Street No. 57, Qufu 273165, China.

The Gobiidae family occupy one of the most diverse habitat ranges of all fishes. One key reason for their successful colonization of different habitats is their ability to adapt to different energy demands. This energy requirement is related to the ability of mitochondria in cells to generate energy via oxidative phosphorylation (OXPHOS). Here, we assembled three complete mitochondrial genomes of , , and These mitogenomes are circular and include 13 protein-coding genes (PCGs), two rRNAs, 22 tRNAs, and one non-coding control region (CR). We used comparative mitochondrial DNA (mtDNA) genome and selection pressure analyses to explore the structure and evolutionary rates of Gobiidae mitogenomics in different environments. The CmC model showed that the ratios of all mtDNA PCGs were <1, and that the evolutionary rate of () was faster in Gobiidae than in other mitochondrial DNA PCGs. We also found evidence of positive selection for several sites of () and genes. Thus, divergent mechanisms appear to underlie the evolution of mtDNA PCGs, which might explain the ability of Gobiidae to adapt to diverse environments. Our study provides new insights on the adaptive evolution of Gobiidae mtDNA genome and molecular mechanisms of OXPHOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani12141741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312210PMC
July 2022

An evolutionary optimization algorithm for proton arc therapy.

Phys Med Biol 2022 Jul 25. Epub 2022 Jul 25.

Radiation Oncology, Beaumont Health System, 3571 W 13 Mile Rd, Royal Oak, Michigan, 48073, UNITED STATES.

Objective: Proton arc plan normally contains thousands of spot numbers and hundreds of energy layers. A recent study reported that the beam delivery time (BDT) is proportional to the spot numbers. Thus, it is critical to find an optimal plan with a fast delivery speed while maintaining a good plan quality. Thus, we developed a novel evolutionary algorithm to directly search for the optimal spot sparsity solution to balance plan quality and BDT.

Approach: The planning platform included a plan quality objective, a generator, and a selector. The generator is based on trust-region-reflective solver. A selector was designed to filter or add the spot according to the expected spot number, based on the user's input of BDT. The generator and selector are used alternatively to optimize a spot sparsity solution. Three clinical cases' CT and structure datasets, e.g. brain, lung, and liver cancer, were used for testing purposes. A series of user-defined BDTs from 15s to 250s were used as direct inputs. The relationship between the plan's cost function value and BDT was evaluated in these three cases.

Main Results: The evolutionary algorithm could optimize a proton arc plan based on clinical user input BDT directly. The plan quality remains optimal in the brain, lung, and liver cases until the BDT was shorter than 25s, 50s and 100s, respectively. The plan quality degraded as the input delivery time became too short, indicating that the plan lacked enough spot or degree of freedom.

Significance: This is the first proton arc planning framework to directly optimize plan quality with the BDT as an input for the new generation of proton therapy systems. This work paved the roadmap for implementing such new technology in a routine clinic and provided a planning platform to explore the trade-off between the BDT and plan quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6560/ac8411DOI Listing
July 2022

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall.

J Vis Exp 2022 Jul 6(185). Epub 2022 Jul 6.

Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University;

Conventional two-dimensional cell culture techniques and animal models have been used in the study of human thoracic aortic aneurysm and dissection (TAAD). However, human TAAD sometimes cannot be characterized by animal models. There is an apparent species gap between clinical human studies and animal experiments that may hinder the discovery of therapeutic drugs. In contrast, the conventional cell culture model is unable to simulate in vivo biomechanical stimuli. To this end, microfabrication and microfluidic techniques have developed greatly in recent years, providing novel techniques for establishing organoids-on-a-chip models that replicate the biomechanical microenvironment. In this study, a human aorta smooth muscle cell organ-on-a-chip (HASMC-OOC) model was developed to simulate the pathophysiological parameters of aortic biomechanics, including the amplitude and frequency of cyclic strain experienced by human aortic smooth muscle cells (HASMCs) that play a vital role in TAAD. In this model, the morphology of HASMCs became elongated in shape, aligned perpendicularly to the strain direction, and presented a more contractile phenotype under strain conditions than under static conventional conditions. This was consistent with the cell orientation and phenotype in native human aortic walls. Additionally, using bicuspid aortic valve-related TAAD (BAV-TAAD) and tricuspid aortic valve-related TAAD (TAV-TAAD) patient-derived primary HASMCs, we established BAV-TAAD and TAV-TAAD disease models, which replicate HASMC characteristics in TAAD. The HASMC-OOC model provides a novel in vitro platform that is complementary to animal models for further exploring the pathogenesis of TAAD and discovering therapeutic targets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3791/64122DOI Listing
July 2022

Comparative Analysis of the Wounded in Patients and Deaths in a Hospital Following the Three Major Earthquakes in Western China.

Front Public Health 2022 8;10:775130. Epub 2022 Jul 8.

Deparment of General Surgery, MianYang Central Hospital, Mianyang, China.

The purpose of this study was to analyze the injury characteristics of patients and therapeutic strategies for patients injured in the last three big earthquakes in China, so as to provide a reference for the improvement of emergency plans for earthquakes. The analysis was based on the data provided by the Mianyang Central Hospital (MCH) from May 12th, 2008 to September 26th, 2017. Microsoft EXCEL software was used for data input, and SPSS was used for statistical analysis. A total of 1,390 earthquake-related patients were hospitalized in MCH. Most patients were admitted to the hospital within the first 2 weeks after the earthquake. The main causes for seismic injuries involved hit/strike by objects or building collapse /burying. Extremity fractures accounted for most injuries, especially 3 days after an earthquake. But soft tissue injuries cannot be neglected. Most earthquake patients were mainly treated by means of surgery and the majority were related to orthopedics. We found that different areas, population, and religions needed a tailored approach to the rescue effort. Therefore, the earthquake magnitude scale has a significant influence on mechanisms, types and severity of the injury of patients injured in earthquakes, as well as their timely transfer, management, and prognosis. Traumatic injuries are very common and thereby various surgical procedures especially orthopedic and neurosurgery are the domain of treatment modalities. Disaster preparedness and combined surgical team effort need to be focused on to reduce both mortality and morbidity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.775130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304578PMC
July 2022

Anti-inflammatory and anti-pruritic effects of Chi-Huang Solution in a murine model of allergic contact dermatitis.

J Ethnopharmacol 2022 Jul 20:115543. Epub 2022 Jul 20.

College of Veterinary Medicine, China Agricultural University, Beijing, 100193, PR China; Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, Beijing, 100193, PR China. Electronic address:

Ethnopharmacological Relevance: In treating atopic dermatitis, multi-mode management is adopted, including trying to avoid the allergens, controlling and preventing secondary infections, and using drugs to control itching. At present, most of the commonly used anti-pruritic drugs in the clinic are single-target and lead to serious side effects. Many studies have shown that a variety of traditional Chinese medicines have significant anti-inflammatory and anti-pruritic effects, and have the characteristics of multiple components, multiple targets, and multiple effects.

Aim Of The Study: The study aimed to explore the anti-inflammatory and anti-pruritic effects of the Chi-Huang Solution in a murine model of Allergic contact dermatitis (ACD). This study considers the effectiveness of the Chi-Huang Solution for external use on skin to provide an experimental basis for the clinical development and application of Chinese medicine and related preparations for Canine atopic dermatitis (CAD).

Materials And Methods: Forty-two male SPF C57BL/6 mice were randomly divided into control group (n = 6), ACD model group (n = 6), HAC control group (n = 6), and 4 Chi-Huang Solution groups (n = 6 in each group). With SADBE induce the murine model of ACD chronic pruritus, and initially evaluate whether the model is successful by counting scratching behavior, measuring the skin fold thickness and skin lesion score within 1 h. After treating the ACD model mice with deionized water, HAC, 1CH, 2CH, 3CH, and 4CH for 7 days, behavioral changes were used to evaluate the anti-pruritic effect. The skin fold thickness, skin lesion score, and spleen index were used to evaluate the anti-inflammatory effect of the Chi-Huang Solution. H.E. staining was used for the epidermal thickness measurement and pathological evaluation. RT-qPCR was used to analyze the mRNA expression of related inflammatory factors such as IL-1β, TNF-α, IL-33, IL-4, IL-17A, CXCL10, and its receptor CXCR3 in the skin of the lesion site, as well as to detect the mRNA expression of pruritus-related genes such as TRPV1, TRPA1, and GRP in DRG.

Results: After the treatment of low-dose (0.1 g/mL) and medium-dose (0.2 g/mL) Chi-Huang Solution, the scratching times both decreased significantly (P < 0.05), meanwhile the medium-dose Chi-Huang Solution had an obvious effect on reducing scratches/scab score (P < 0.05). Moreover, no matter what dose it takes, all Chi-Huang Solution can alleviate the epidermal thickening (P < 0.05) and the infiltration of mast cells in the ACD murine model of ACD. It is worth mentioning that the count of mast cells in the dermis was significantly down-regulated after the treatment of medium-dose Chi-Huang Solution (P < 0.005). Furthermore, Chi-Huang Solution can significantly down-regulate the mRNA expression of related inflammatory factors in the skin, and reduce the mRNA expression of pruritus-related genes, such as TRPA1, TRPV1, and GRP in the spinal cord.

Conclusions: The results indicated that Chi-Huang Solution for external use exhibits significant anti-inflammatory and anti-pruritic effects on SADBE-induced ACD chronic pruritus murine models. Chi-Huang Solution might emerge as an effective drug for the treatment of CAD and high-dose Chi-Huang Solution (0.4 g/ml) has better comprehensive effects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2022.115543DOI Listing
July 2022

Arthrostoma leucurus sp. n. (Nematoda: Ancylostomatidae), A New Hookworm Species Isolated from Asian Badger in China.

Acta Parasitol 2022 Jul 23. Epub 2022 Jul 23.

School of Medicine, Shihezi University, Shihezi, 832002, Xinjiang Uygur Autonomous Region, People's Republic of China.

Purpose: To date, ten validated Arthrostoma species were reported. Here, a new hookworm species was found from Asian badger (Meles leucurus).

Methods: Nineteen hookworms (9 males and 10 females) were collected from the small intestine of two Asian badgers in Xinjiang Uygur Autonomous Region, northwestern China. The hookworms were morphologically examined according to key taxonomic characters, such as anterior extremity direction, structures of oral opening (cutting plates or teeth), vulva location, buccal capsule anatomy (integrated or formed by articulating plates), the length of spicule and gubernaculum, number of plates of buccal capsule, and presence or absence of vulvar papillae.

Results: The hookworm species from Asian badger, here named as Arthrostoma leucurus sp. n., was different from the previously described ten Arthrostoma species. The phylogenetic tree based on the cox1 gene showed that Arthrostoma leucurus sp. n. formed a separate clade, as a sister group to Ancylostoma and Uncinaria species.

Conclusion: Arthrostoma leucurus sp. n., the eleven validated Arthrostoma species, was identified from Asian badger in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11686-022-00587-5DOI Listing
July 2022

Babam2 negatively regulates osteoclastogenesis by interacting with Hey1 to inhibit Nfatc1 transcription.

Int J Biol Sci 2022 11;18(11):4482-4496. Epub 2022 Jul 11.

Guangzhou Jinan Biomedicine Research and Development Center, Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

Osteoclast-mediated excessive bone resorption was highly related to diverse bone diseases including osteoporosis. BRISC and BRCA1-A complex member 2 (Babam2) was an evolutionarily conserved protein that is highly expressed in bone tissues. However, whether Babam2 is involved in osteoclast formation is still unclear. In this study, we identify Babam2 as an essential negative regulator of osteoclast formation. We demonstrate that Babam2 knockdown significantly accelerated osteoclast formation and activity, while Babam2 overexpression blocked osteoclast formation and activity. Moreover, we demonstrate that the bone resorption activity was significantly downregulated in Babam2-transgenic mice as compared with wild-type littermates. Consistently, the bone mass of the Babam2-transgenic mice was increased. Furthermore, we found that Babam2-transgenic mice were protected from LPS-induced bone resorption activation and thus reduced the calvarial bone lesions. Mechanistically, we demonstrate that the inhibitory effects of Babam2 on osteoclast differentiation were dependent on Hey1. As silencing Hey1 largely diminished the effects of Babam2 on osteoclastogenesis. Finally, we show that Babam2 interacts with Hey1 to inhibit Nfatc1 transcription. In sum, our results suggested that Babam2 negatively regulates osteoclastogenesis and bone resorption by interacting with Hey1 to inhibit Nfatc1 transcription. Therefore, targeting Babam2 may be a novel therapeutic approach for osteoclast-related bone diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.72487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295054PMC
July 2022

Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.

J Appl Microbiol 2022 Jul 20. Epub 2022 Jul 20.

Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.

Aims: Diarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the causative agents, may therefore improve confidence in associating a pathogen to the disease. This study evaluated a novel commercially available, multiplex quantitative polymerase chain reaction (qPCR) assay (Enterit4Calves) for detection and quantification of pathogens associated with calf-diarrhoea.

Methods And Results: Performance of the method was first evaluated under laboratory conditions. Then it was compared with current routine methods for detection of pathogens in faecal samples from 65 calves with diarrhoea and in 30 spiked faecal samples. The qPCR efficiencies were between 84%-103% and detection limits of 100-1000 copies of nucleic acids per sample were observed. Correct identification was obtained on 42 strains of cultured target bacteria, with only one false positive reaction from 135 nontarget bacteria. Kappa values for agreement between the novel assay and current routine methods varied between 0.38 and 0.83.

Conclusion: The novel qPCR method showed good performance under laboratory conditions and a fair to good agreement with current routine methods when used for testing of field samples.

Significance And Impact Of Study: In addition to having fair to good detection abilities, the novel qPCR method allowed quantification of pathogens. In the future, use of quantification may improve diagnosis and hence treatment of calf diarrhoea.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jam.15722DOI Listing
July 2022

Lamprey immunity protein enables early detection and recurrence monitoring for bladder cancer through recognizing Neu5Gc-modified uromodulin glycoprotein in urine.

Biochim Biophys Acta Mol Basis Dis 2022 Jul 16;1868(12):166493. Epub 2022 Jul 16.

College of Life Science, Liaoning Normal University, Dalian, Liaoning, China. Electronic address:

The clinical management of bladder cancer (BCa) is hindered by the lack of reliable biomarkers. We aimed to investigate the potential of lamprey immunity protein (LIP), a lectin that specifically binds to multi-antennary sialylated N-glycolylneuraminic acid (Neu5Gc) structures on UMOD glycoproteins in the urine of BCa patients. Primary BCa patients had higher levels of LIP-bound Neu5Gc in urine than healthy participants and patients receiving postoperative treatment did. In addition, lectin chip assay and mass spectrometry were used to analyze the glycan chain structure, which can recognize the UMOD glycoprotein decorated with multi-antennary sialylated Neu5Gc structures. Furthermore, compared with urine samples from healthy patients (N = 2821, T/C = 0.12 ± 0.09) or benign patients (N = 360, T/C = 0.11 ± 0.08), the range of the urine T/C ratio detected using LIP test paper was 1.97 ± 0.32 in patients with bladder cancer (N = 518) with significant difference (P < 0.0001). Our results indicate that LIP may be a tool for early BCa identification, diagnosis, and monitoring. Neu5Gc-modified UMOD glycoproteins in urine and Neu5Gc-modified N-glycochains and sialyltransferases may function as potential markers in clinical trials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbadis.2022.166493DOI Listing
July 2022
-->