Publications by authors named "Gachechiladze M"

40 Publications

Correlation between BRCA1 expression and clinicopathological factors including brain metastases in patients with non-small-cell lung cancer.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2013 Sep 1;157(3):227-32. Epub 2012 Nov 1.

Laboratory of Molecular Pathology, Department of Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.

Background: Previously identified as a breast and ovarian cancer susceptibility gene, BRCA1 has gained major scientific interest as a potential prognostic and/or predictive marker for various tumors, including non-small-cell lung cancer (NSCLC), the leading cause of cancer related mortality worldwide. BRCA1 plays a central role in DNA damage response (DDR. It undergoes phosphorylation by various DDR kinases at different serine residues, of which ser1524 is known to be specifically phosphorylated by ATM in response to genotoxic stress.

Methods: We performed BRCA1 immunohistochemistry on several tissue microarrays (TMAs) of 113 early (I, II stage) and advanced (III, IV stage) NSCLCs, using MS110 antibody against the BRCA1 N-terminal and S1524 antibody against the phosphorylated form of BRCA1 protein at ser1524 (Abcam). Patients with III and IV stage disease were treated by adjuvant cisplatin-based chemotherapy. Staining results were correlated with overall survival (OS), disease free survival (DFS) and with the occurrence of brain metastases.

Results: BRCA1 S1524 nuclear positivity was significantly correlated with longer OS and DFS in stage I and II patients (P<0.05), while OS and DFS were shorter in S1524 positive stage III and IV patients (P<0.05). No significant correlation was found with brain metastases.

Conclusion: The results show that BRCA1 phosphorylaton, at least in ser1524, differentiates the fate of early and advanced NSCLC as well as response to chemotherapy, but the underlying mechanisms are not completely understood. Detection of phosphorylated forms of BRCA1 might serve as a useful prognostic and predictive marker for patients with NSCLC.
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http://dx.doi.org/10.5507/bp.2012.099DOI Listing
September 2013

The role of BRCA1 in non-small cell lung cancer.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2012 Sep 1;156(3):200-3. Epub 2012 Jun 1.

Department of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.

Background: BRCA1 (Breast cancer 1) was previously identified as a breast and ovarian cancer susceptibility gene, but recently gained a major scientific interest as a prognostic and/or predictive marker for various tumors, including non-small cell lung cancer (NSCLC), which is the leading cause of cancer related mortality in the world. We aimed to review the role of BRCA1 in NSCLC based on currently available literature.

Methods: We performed the literature search in Pubmed database, using key words: BRCA1, non small cell lung cancer, chemotherapy, drug resistance. Articles published in English were selected for review.

Results: Research papers are mainly focused on BRCA1 mRNA expression studies in response to DNA damaging chemotherapy. Several articles about genetic and epigenetic changes of BRCA1 in NSCLC were also available.

Conclusions: BRCA1 is a multifunctional tumor supressor protein, which plays a key role in essential cellular processes and modulates the cellular response to cytotoxic chemotherapy. With the difference from breast and ovarian cancer, BRCA1 has no role in NSCLC cancerogenesis and mainly discussed as a promising genomic marker for customized chemotherapy in NSCLC patients.
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http://dx.doi.org/10.5507/bp.2012.049DOI Listing
September 2012

Cytologic, colposcopic and histopathologic correlations of hyperkeratosis in reproductive women.

Georgian Med News 2011 Nov;11(200):121-4

Research Institute of Clinical Medicine, Department of Gynecology, Tbilisi, Georgia.

The significance of leucoplakia/hyperkeratosis in colposposcopy or hyperkeratotic cells in Pap smear, is not fully understood. It is considered that hyperkeratosis itself is a benign change, but it may mask other pathologies, including invasive carcinoma. We studied 123 woman with colposcopically identified hyperkeratosis and its' possible relationship with dysplastic lesions of uterine cervix, based on correlation with cytopathological and histopathological data. The results of our study showed that 1) cervical dysplastic lesions are identified 2 times less frequently in conventional Pap smears from patients with thick leukoplakia, than in patients with thin leukoplakia and 2) histopathologically identified cervical dysplasias are 1,5 times more common in patients with thick leukoplakia. Pathologists and gynecologists in practice should be aware of this difference.
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November 2011

[Lynch syndrome (case report)].

Georgian Med News 2009 Apr(169):7-9

The authors have analyzed the ethology, pathogenesis, treatment of Lynch Syndrome and described a case history of a 65-year-old woman with right breast cancer who was admitted into a hospital with intestines impassability and operation was performed. During the operation tumour, which caused the acute intestine impassability was identified. Apart from this, after the inner organ examination metastasis on stomach and tumour in upper and middle rectum were found. Histology and colonoscopy revealed the rectum adenocarcinoma (morphologic code 8140/3) with mild differentiation. Examination revealed adenocarcinoma with high and middle area differentiation (morphologic code 8480/3). Post-operational period was satisfactory. On the 10-th day the stitches were removed, chemical therapy was carried out according to the scheme set. Patient was examined after a year and two months. General health was satisfactory; she conducts an active life.
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April 2009

[Value of thrombolytic therapy in acute myocardial ischemia after administration of kinin system inhibitors].

Kardiologiia 1986 Sep;26(9):37-41

An experimental study in dogs showed that thrombolytic revascularization after 4 to 6 hours of coronary arterial occlusion resulted in further deterioration of ischemia and an impairment of cardiac contractility and hemodynamics. Micromorphologically, multiple extravasations were demonstrated in the ischemized region in combination with insignificant glycogen content. The administration of thrombolytics was accompanied by enhanced fibrinolytic activity, yet the activation of the first three phases of blood coagulation showed no decline. In a series with thrombolytic therapy preceded by contrykal and heparin infusions, micromorphology revealed no intramyocardial hemorrhages in the ischemized region. Active functional cells showed micro- and macrogranular glycogen that accumulated into clusters in myocardial fibres. The significant postrevascularization decline in ischemia was accompanied by improved myocardial contractility and cardiac hemodynamics in the presence of hypocoagulation and increased fibrinolytic activity.
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September 1986

[Radioactive iodine in the therapy of thyrotoxicoses].

Probl Endokrinol Gormonoter 1960 Jan-Feb;6:102-6

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November 1998