Dr GOKHALE D, PhD - National Chemical Laboratory

Dr GOKHALE D

PhD

National Chemical Laboratory

Pune, Maharashtra | India

Main Specialties: Biology, Biotechnology

Additional Specialties: Microbial Technology, Biochemistry, Microbiology

ORCID logohttps://orcid.org/0000-0002-2895-2869


Top Author

Dr GOKHALE D, PhD - National Chemical Laboratory

Dr GOKHALE D

PhD

Introduction

Presently, I am working as consultant in CSIR-National Chemical laboratory. My areas of interest are microbial strain improvement, enzyme technology and fermentation, Biotransformation. I have more than 80 publications in highly reputed journals and 6 US patents to my credit. I am also a professor, AcSIR, New Delhi.

Primary Affiliation: National Chemical Laboratory - Pune, Maharashtra , India

Specialties:

Additional Specialties:

Research Interests:


View Dr GOKHALE D’s Resume / CV

Education

Oct 1988
Pune University
Ph. D

Experience

CSIR-National Chemical Laboratory

Publications

86Publications

271Reads

6Profile Views

82PubMed Central Citations

Lignocellulosic biomass: Hurdles and challenges in its valorization.

Appl Microbiol Biotechnol 2019 Dec 9;103(23-24):9305-9320. Epub 2019 Nov 9.

NCIM Resource Center, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411008, India.

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http://dx.doi.org/10.1007/s00253-019-10212-7DOI Listing
December 2019
3.337 Impact Factor

Polylactic acid: synthesis and biomedical applications.

J Appl Microbiol 2019 Dec 17;127(6):1612-1626. Epub 2019 Jun 17.

CSIR-National Chemical Laboratory, NCIM Resource Centre, Pune, India.

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http://dx.doi.org/10.1111/jam.14290DOI Listing
December 2019
3 Reads
2.479 Impact Factor

of enzymes as tools in industrial processes

Recent Patents on Biotechnology 12(4), 297-298

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2018
4 Reads

Greener L-lactic acid production through in situ extractive fermentation by an acid-tolerant Lactobacillus strain.

Appl Microbiol Biotechnol 2018 Aug 24;102(15):6425-6435. Epub 2018 May 24.

Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-Ku, Fukuoka, 812-8581, Japan.

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http://dx.doi.org/10.1007/s00253-018-9084-4DOI Listing
August 2018
10 Reads
3.340 Impact Factor

Pseudozyma hubeiensis, an unexplored yeast: It’s potential in biomass conversion to value added products

J Bacteriol. Mycol. (Open Access) 6(2): 84-87

J. Bacteriol. Mycol. (Open Access)

There are no reports available for the production of industrially important enzymes and other biological products from P. hubeiensis and hence it remained still unexplored. P. hubeiensis was first isolated from sandal wood in our laboratory which produced a complete cellulase free xylanolytic system consisting of endoxylanase and â-xylosidase. The purified xylanases produced smaller chain length (X3-X7) xylooligosaccharides (XOS) which have great potential use as prebiotic in functional food. In addition, it is also used in cosmetics, pharmaceuticals or agricultural products and as a plant growth regulator. It also produces high amounts of metal and ethanol tolerant â-xylosidase qualifying its use in lignocellulosic biomass hydrolysis when mixed with enzyme preparations that are deficient in â-xylosidase. P. hubeiensis utilizes simultaneously all the sugars present in biomass hydrolysate and convert them to lipids. In this mini-review, the biology and enzymology underlying the biomass degrading enzymes and the production of lipids/biosurfactants especially MELs are described. An approach to developing P. hubeiensis strains for production of biomass degrading enzymes and their application is outlined.

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April 2018
6 Reads

Use of enzymes as tools in industrial processes

Recent Patents on Biotechnology 12(4): 297-298

Recent Patents on Biotechnology

Review Perspective

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April 2018
7 Reads

Purification and characterization of β-Glucosidase from Penicillium janthinellum Mutant EU2D-21

Current Biotechnology 6, 356-363, DOI:10.2174/2211550106666170126155150

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November 2017
7 Reads

Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast.

AMB Express 2016 Dec 15;6(1):73. Epub 2016 Sep 15.

NCIM Resource Center, CSIR-National Chemical Laboratory, Pune, Maharashtra, 411008, India.

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http://dx.doi.org/10.1186/s13568-016-0243-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023640PMC
December 2016
12 Reads

Phenyllactic acid: A potential antimicrobial compound in lactic acid bacteria

J Bacteriol Mycol Open Access 2016 Oct 2(5):00037

J. Bacteriol. Mycol. (Open Access)

against bacteria and fungi. PhLA exists in two chiral isomers, L-PhLA and D-PhLA which show antimicrobial activity. D-PhLA shows more antimicrobial activity than L-PhLA and hence it is receiving great attention as food and feed additive in place antibiotics which control the microbial contamination and thereby increase the shelf life of food and food ingredients. This review summarizes the recent developments on the resources, detection and analysis and antimicrobial activity of PhLA. In addition, the article presents the recent studies of the key enzymes such as lactate dehydrogenases and pyruvate reductases involved in PhLA synthesis. The metabolic pathway and regulation of PLA synthesis in lactic acid bacteria (LAB) along with its high level production are also discussed.

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October 2016
9 Reads

Biocatalyst development for lactic acid production at acidic pH using inter-generic protoplast fusion.

RSC Advances 2015, 5: 2024-2031

RSC Advances

Global warming and environmental problems force us to develop sustainable processes based on the use of biocatalysts that are eco-friendly with the least potential toxicity. Lactic acid fermentation at neutral pH generates a large amount of gypsum during down-stream processing. Hence it is essential to develop Lactobacillus strains which produce lactic acid at acidic pH thus making the whole downstream process environmentally friendly. Fusant F3 was generated using protoplast fusion between Lactobacillus delbrueckii Mut Uc-3 and Acetobacter pasteurianus NCIM 2314 on solid media at pH 4.0. Fusant F3 was further treated by UV irradiation to generate a mutant, FM1, with improvements in acid tolerance which produced five-fold more lactic acid than the parent strain at acidic pH. The molecular studies using RAPD markers demonstrated that the fusant is derived from both the parental strains, Acetobacter and Lactobacillus and the mutant is derived from the fusant. The utilization of such acid tolerant strains could be able to produce free lactic acid at acidic pH without using neutralizing agents and will offer an effective means for designing environmentally benign processes for lactic acid production.

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July 2015
8 Reads

Biomass exploitation: A challenge finding its way to reality.

Current Science 2015, 108(9), 1593-1594

Current Science

Lignocellulosic biomass holds the key to supplying the basic needs of society for sustainable production of chemicals and fuels without impacting the human food supply. The production of second-generation biofuels and chemicals from lignocellulosic biomass has not yet been commercialized due to its complex and recalcitrance structure. Therefore, the challenges involved in the production of lignocellulosic biomass-derived fuels and chemicals must be addressed. Search for economic pretreatment methods has been recognized as one of the main hurdles for processing of biomass to biofuels and chemicals. The conversion of all biomass components, lignin in particular, would greatly contribute to the economic viability of biomass-based processes for second-generation biofuels and chemicals. Hydrolysis of lignocellulose carbohydrates into fermentable sugars requires a suitable cellulase enzyme cocktail acting on both raw as well as pretreated biomass. Depending on raw material and pretreatment technology, the enzyme mixtures must be designed to degrade biomass carbohydrates. Recent publications on GVL-pretreatment to solubilize and degrade carbohydrates in biomass and CelA enzyme acting on raw biomass would probably meet the challenges in biomass conversion technologies.

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May 2015
7 Reads

Lignicellulose processing: a current challenge

RSC Advances 2014, 4: 8271-8277

RSC Advances

This review covers the use of biochemical techniques for biomass processing, including biostrategies for the development of commercial processes for the generation of chemicals such as biofuels.

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February 2014
8 Reads

Optimization studies for enhancing cellulase production by Penicillium janthinellum mutant EU2D-21 using Response Surface Methodology

BioResources 2014, 9(2): 1914-1923

BioResources

Extracellular fungal cellulases are key enzymes for the degradation of lignocellulosic biomass. In this study, the basal medium for cellulase production by Penicillium janthinellum mutant (EU2D-21) in submerged fermentation conditions was optimized using RSM technique.

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February 2014
11 Reads

Supplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity.

Bioresour Technol 2013 Oct 20;146:736-739. Epub 2013 Jul 20.

NCIM Resource Center, CSIR-National Chemical Laboratory, Pune 411008, India. Electronic address:

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http://dx.doi.org/10.1016/j.biortech.2013.07.057DOI Listing
October 2013
13 Reads
1 Citation
4.494 Impact Factor

Differential induction, purification and characterization of cold active lipase from Yarrowia lipolytica NCIM 3639.

Bioresour Technol 2011 Nov 10;102(22):10663-70. Epub 2011 Sep 10.

NCIM Resource Center, National Chemical Laboratory (CSIR), Maharashtra, Pune 411008, India.

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http://dx.doi.org/10.1016/j.biortech.2011.09.013DOI Listing
November 2011
10 Reads
2 Citations
4.494 Impact Factor

Comparative production of cellulases by mutants of Penicillium janthinellum NCIM 1171 and its application in hydrolysis of Avicel and cellulose.

Bioresour Technol 2011 Jun 14;102(11):6569-72. Epub 2011 Jan 14.

NCIM Resource Center, National Chemical Laboratory (CSIR), Pune 411008, Maharashtra, India.

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http://dx.doi.org/10.1016/j.biortech.2011.01.014DOI Listing
June 2011
9 Reads
2 Citations
4.494 Impact Factor

Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass.

Bioresour Technol 2011 Mar 7;102(6):4304-12. Epub 2011 Jan 7.

NCIM Resource Center, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.

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http://dx.doi.org/10.1016/j.biortech.2011.01.002DOI Listing
March 2011
11 Reads
16 Citations
4.494 Impact Factor

Lipase of Aspergillus niger NCIM 1207: A Potential Biocatalyst for Synthesis of Isoamyl Acetate.

Indian J Microbiol 2010 Oct 25;50(4):432-7. Epub 2011 Jan 25.

NCIM Resource Center, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008 Maharashtra India.

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http://dx.doi.org/10.1007/s12088-011-0087-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209843PMC
October 2010
9 Reads
0.900 Impact Factor

Strain improvement of Lactobacillus lactis for D-lactic acid production.

Biotechnol Lett 2010 Apr 24;32(4):517-20. Epub 2009 Dec 24.

NCIM Resource Center, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, Maharashtra, India.

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http://dx.doi.org/10.1007/s10529-009-0187-yDOI Listing
April 2010
11 Reads
3 Citations
1.591 Impact Factor

Protoplast formation and regeneration in Lactobacillus delbrueckii.

Indian J Microbiol 2010 Mar 5;50(1):97-100. Epub 2010 Mar 5.

National Chemical Laboratory, NCIM Resource Center, Pune, 411 008 Maharashtra India.

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http://dx.doi.org/10.1007/s12088-010-0008-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3450288PMC
March 2010
13 Reads
0.900 Impact Factor

Biochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides.

Bioresour Technol 2009 Dec 18;100(24):6488-95. Epub 2009 Aug 18.

NCIM Resource Center, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411 008, Maharashtra, India.

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http://dx.doi.org/10.1016/j.biortech.2009.07.064DOI Listing
December 2009
12 Reads
6 Citations
4.494 Impact Factor

Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207.

Bioresour Technol 2009 Feb 1;100(3):1486-90. Epub 2008 Oct 1.

National Chemical Laboratory, NCIM Resource Center, Maharashtra, Pune, India.

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http://dx.doi.org/10.1016/j.biortech.2008.08.016DOI Listing
February 2009
8 Reads
7 Citations
4.494 Impact Factor

Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger.

Int J Biol Macromol 2008 Dec 22;43(5):422-5. Epub 2008 Aug 22.

Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India.

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http://dx.doi.org/10.1016/j.ijbiomac.2008.08.005DOI Listing
December 2008
7 Reads
2.860 Impact Factor

Utilization of molasses sugar for lactic acid production by Lactobacillus delbrueckii subsp. delbrueckii mutant Uc-3 in batch fermentation.

Appl Environ Microbiol 2008 Jan 2;74(1):333-5. Epub 2007 Nov 2.

NCIM, National Chemical Laboratory, Pune 411 008, Maharashtra, India.

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http://dx.doi.org/10.1128/AEM.01595-07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223217PMC
January 2008
30 Reads
7 Citations
3.670 Impact Factor

Metal complexes of crosslinked chitosans Part II. An investigation of their hydrolysis to chitooligosaccharides using chitosanase.

Int J Biol Macromol 2007 Dec 30;41(5):491-6. Epub 2007 Jun 30.

Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India.

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http://dx.doi.org/10.1016/j.ijbiomac.2007.06.009DOI Listing
December 2007
9 Reads
1 Citation
2.860 Impact Factor

Production of lactic acid from cellobiose and cellotriose by Lactobacillus delbrueckii mutant Uc-3.

Appl Environ Microbiol 2007 Aug 8;73(15):5055-7. Epub 2007 Jun 8.

NCIM, National Chemical Laboratory, Pune 411 008, Maharashtra, India.

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http://dx.doi.org/10.1128/AEM.00774-07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1951004PMC
August 2007
10 Reads
4 Citations
3.670 Impact Factor

Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulase production.

Bioresour Technol 2007 May 13;98(7):1467-73. Epub 2006 Nov 13.

NCIM Resource Center, National Chemical Laboratory, Pune, 411 008 Maharashtra, India.

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http://dx.doi.org/10.1016/j.biortech.2006.02.036DOI Listing
May 2007
12 Reads
20 Citations
4.494 Impact Factor

Production of lactic acid and fructose from media with cane sugar using mutant of Lactobacillus delbrueckii NCIM 2365.

Lett Appl Microbiol 2006 Jul;43(1):53-7

NCIM Resource Centre, National Chemical Laboratory, Pune, Maharashtra, India.

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http://dx.doi.org/10.1111/j.1472-765X.2006.01907.xDOI Listing
July 2006
14 Reads
1 Citation
1.660 Impact Factor

Protoplast fusion: a tool for intergeneric gene transfer in bacteria.

Biotechnol Adv 1993 ;11(2):199-217

Department of Biochemical Sciences, National Chemical Laboratory, Pune, India.

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http://dx.doi.org/10.1016/0734-9750(93)90041-kDOI Listing
December 2003
7 Reads
2 Citations
9.020 Impact Factor

Towards biodegradable polyolefins: strategy of anchoring minute quantities of monosaccharides and disaccharides onto functionalized polystyrene, and their effect on facilitating polymer biodegradation.

Chem Commun (Camb) 2002 Dec(23):2884-5

Polymer Science & Engineering Group, Chemical Engineering Division, National Chemical Laboratory, Pune 411008, India.

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http://dx.doi.org/10.1039/b209254aDOI Listing
December 2002
12 Reads
1 Citation
6.834 Impact Factor

Chemoenzymatic synthesis of D(-)phenylglycine using hydantoinase of Pseudomonas desmolyticum resting cells.

Enzyme Microb Technol 1996 Apr;18(5):353-7

Division of Biochemical Sciences, National Chemical Laboratory, Pune, Maharashtra, India.

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http://dx.doi.org/10.1016/0141-0229(95)00127-1DOI Listing
April 1996
10 Reads
2.322 Impact Factor

Industrial yeast strain improvement: construction of a highly flocculent yeast with a killer character by protoplast fusion.

J Ind Microbiol 1995 Aug;15(2):94-102

Division of Biochemical Sciences, National Chemical Laboratory, Pune, Maharashtra, India.

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http://dx.doi.org/10.1007/BF01569806DOI Listing
August 1995
7 Reads
5 Citations

Isolation of intergeneric hybrids between Bacillus subtilis and Zymomonas mobilis and the production of thermostable amylase by hybrids.

Biotechnol Appl Biochem 1994 08;20(1):109-16

NCIM, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.

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August 1994
8 Reads
1.362 Impact Factor

Protoplast fusion in Yeast : Strain improvement in Saccharomyces

Indian Journal of Microbiology 32 : 15-27

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1992
5 Reads

Protection of Aspergillus niger cellulases by urea during growth on glucose or glycerol supplemented media.

Appl Biochem Biotechnol 1992 Oct;37(1):11-7

NCIM Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.

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http://dx.doi.org/10.1007/BF02788853DOI Listing
October 1992
8 Reads
1.740 Impact Factor

Optimization of cellulase production by Aspergillus niger NCIM 1207.

Appl Biochem Biotechnol 1991 Jul;30(1):99-109

NCIM, Division of Biochemical Sciences, Pune, India.

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http://dx.doi.org/10.1007/BF02922026DOI Listing
July 1991
9 Reads
3 Citations
1.740 Impact Factor

Differential Expression of Xylanases and Endoglucanases in the Hybrid Derived from Intergeneric Protoplast Fusion between a Cellulomonas sp. and Bacillus subtilis.

Appl Environ Microbiol 1989 Oct;55(10):2675-80

NCIM, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, and Department of Zoology, University of Poona, Pune 411 007, India.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC203143PMC
October 1989
7 Reads
1 Citation
3.670 Impact Factor

Fungal protoplast fusion : A tool for breeding industrial strains

Journal of Scientific and Industrial Research 51 : 497-506

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15 Reads

Protoplast formation and regeneration in Acetobacter pasteurianus.

American Journal of Bioengineering and Biotechnology 1: 37-43

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17 Reads

Top co-authors

Digambar Gokhale
Digambar Gokhale

NCIM Resource Center

6
Jayant Khire
Jayant Khire

CSIR-National Chemical Laboratory (CSIR-NCL)

2
Mukund Adsul
Mukund Adsul

RMIT University

2
Mamata Singhvi
Mamata Singhvi

NCIM Resource Center

2
Takeshi Zendo
Takeshi Zendo

Kyushu University

1
Anjani J Varma
Anjani J Varma

CSIR - National Chemical Laboratory

1
Padmaja Galgali
Padmaja Galgali

National Chemical Laboratory

1