Publications by authors named "G d"

12 Publications

Role of membrane proteins in bacterial synthesis of hyaluronic acid and their potential in industrial production.

Int J Biol Macromol 2020 Dec 10;164:1916-1926. Epub 2020 Aug 10.

Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India. Electronic address:

Hyaluronic acid (HA) is a glycosaminoglycan polymer found in various parts of human body and is required for functions like lubrication, water homeostasis etc. Hyaluronic acid is mostly produced industrially by bacterial fermentation for pharmaceutical and cosmetic applications. This review discusses on the role of membrane proteins involved in synthesis and transport of bacterial HA, since HA is a transmembrane product. The different types of membrane proteins involved, their transcriptional control in wild type bacteria and the expression of those proteins in various recombinant hosts have been discussed. The role of phospholipids and metal ions on membrane proteins activity, HA yield and size of HA have also been discussed. Today with an estimated market of US$ 8.3 billion and which is expected to grow to US$ 15.25 billion in 2026, it is essential to increase the efficiency of the industrial HA production process. So this review also proposes on how those membrane proteins and cellular mechanisms like the transcriptional control can be utilised to develop efficient industrial strains that enhance the yield and size of HA produced.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.077DOI Listing
December 2020

Lockdown measures and relative changes in the age-specific incidence of SARS-CoV-2 in Spain.

medRxiv 2020 Jul 2. Epub 2020 Jul 2.

Department of Epidemiology and Department of Biostatistics, Harvard T.H. Chan School of Public Health; Harvard-MIT Division of Health Sciences and Technology, Boston, United States.

Background: The first months of the SARS-CoV-2 epidemic in Spain resulted in high incidence and mortality. A national sero-epidemiological survey suggests higher cumulative incidence of infection in older individuals than in younger individuals. However, little is known about the epidemic dynamics in different age groups, including the relative effect of the lockdown measures introduced on March 15, and strengthened on March 30 to April 14, 2020 when only essential workers continued to work.

Methods: We used data from the National Epidemiological Surveillance Network (RENAVE in Spanish) on the daily number of reported COVID-19 cases (by date of symptom onset) in eleven 5-year age groups: 15-19y through 65-69y. For each age group g, we computed the proportion E(g) of individuals in age group g among all reported cases aged 15-69y during the pre-lockdown period (March 1-10, 2020) and the corresponding proportion L(g) during two lockdown periods (March 25-April 3 and April 8-17, 2020). For each lockdown period, we computed the proportion ratios PR(g)= L(g)/E(g). For each pair of age groups g1,g2, PR(g1)>PR(g2) implies a relative increase in the incidence of detected SARS-CoV-2 infection in the age group g1 compared with g2 for the later vs. early period.

Results: For the first lockdown period, the highest PR values were in age groups 50-54y (PR=1.21; 95% CI: 1.12,1.30) and 55-59y (PR=1.19; 1.11,1.27). For the second lockdown period, the highest PR values were in age groups 15-19y (PR=1.26; 0.95,1.68) and 50-54y (PR=1.20; 1.09,1.31).

Conclusions: Our results suggest that different outbreak control measures led to different changes in the relative incidence by age group. During the first lockdown period, when non-essential work was allowed, individuals aged 40-64y, particularly those aged 50-59y presented with higher COVID-19 relative incidence compared to pre-lockdown period, while younger adults/older adolescents (together with persons aged 50-59y) had increased relative incidence during the later, strengthened lockdown. The role of different age groups during the epidemic should be considered when implementing future mitigation efforts.
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http://dx.doi.org/10.1101/2020.06.30.20143560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340201PMC
July 2020

Study of electrolytes in patients of Dengue in a tertiary care hospital in India.

J Assoc Physicians India 2020 Jan;68(1):85

HBT Medical College, Mumbai.

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January 2020

Hydration dynamics of collagen in aqueous buffer solution as studied by time domain dielectric spectroscopy.

Int J Biol Macromol 2018 Oct 10;118(Pt B):1811-1816. Epub 2018 Jul 10.

School of Physical Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431606, Maharashtra, India.

Dielectric relaxation studies of collagen in aqueous buffer solution (pH 3.7) have been carried out at four different temperatures 283, 288, 293 and 298 K as a function of collagen concentration between 0.033 μM and 0.2 μM in the frequency range of 1 GHz to 25 GHz using time domain dielectric spectroscopy technique. Apart from the dominant mode at high frequency around 14 GHz which is assigned to the free water relaxation; we have detected one more pronounced peak close to 1.8 GHz in the δ-dispersion range of the loss spectrum of collagen in aqueous buffer solution. The peak around 1.8 GHz is attributed to hydration water reorientation of the collagen macromolecules and has obtained detailed information on its temperature and concentration dependence. The retardation imposed on the hydration water by collagen is found to be ≈4.7, since collagen can cause long range perturbations beyond the first hydration shell. The activation enthalpy and activation entropy for the dipolar orientation for collagen at different concentrations have been calculated from the Arrhenius plot and is found to be 32 kJ/mol and 4.2 J/mol K respectively. The increase of thermodynamic activation enthalpy and decrease of activation entropy of collagen in buffer in the present study compared to the water have supported the idea that the water associated with collagen is highly ordered.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.07.020DOI Listing
October 2018

Managing diabetic ketoacidosis in pregnancy.

Saudi J Anaesth 2016 Apr-Jun;10(2):238-9

Department of Anaesthesiology and Perioperative Medicine, Complejo Hospitalario Universitario de A Coruña, A Coruña, Spain.

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http://dx.doi.org/10.4103/1658-354X.168829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799622PMC
April 2016