Publications by authors named "Fred W Perrino"

45Publications

TREX1 - Apex predator of cytosolic DNA metabolism.

DNA Repair (Amst) 2020 Oct 12;94:102894. Epub 2020 Jun 12.

Department of Biochemistry, Center for Structural Biology, Wake Forest School of Medicine, Winston-Salem, NC 27157, United States. Electronic address:

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http://dx.doi.org/10.1016/j.dnarep.2020.102894DOI Listing
October 2020

T Cells Produce IFN-α in the TREX1 D18N Model of Lupus-like Autoimmunity.

J Immunol 2020 01 11;204(2):348-359. Epub 2019 Dec 11.

Department of Biochemistry, Center for Structural Biology, Wake Forest School of Medicine, Winston-Salem, NC 27157;

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http://dx.doi.org/10.4049/jimmunol.1900220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946867PMC
January 2020

Measuring TREX1 and TREX2 exonuclease activities.

Methods Enzymol 2019 8;625:109-133. Epub 2019 Jun 8.

Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC, United States. Electronic address:

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http://dx.doi.org/10.1016/bs.mie.2019.05.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893858PMC
June 2020

TREX1 D18N mice fail to process erythroblast DNA resulting in inflammation and dysfunctional erythropoiesis.

Autoimmunity 2018 Nov 13;51(7):333-344. Epub 2018 Nov 13.

Department of Biochemistry, Center for Structural Biology, Wake Forest School of Medicine, Winston-Salem, NC, USA.

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http://dx.doi.org/10.1080/08916934.2018.1522305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513733PMC
November 2018

Identification of Inhibitors of the dNTP Triphosphohydrolase SAMHD1 Using a Novel and Direct High-Throughput Assay.

Biochemistry 2018 11 13;57(47):6624-6636. Epub 2018 Nov 13.

Center for Structural Biology, Department of Biochemistry , Wake Forest School of Medicine , Winston-Salem , North Carolina 27157 , United States.

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http://pubs.acs.org/doi/10.1021/acs.biochem.8b01038
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http://dx.doi.org/10.1021/acs.biochem.8b01038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322912PMC
November 2018

Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.

Nano Lett 2017 11 5;17(11):7110-7116. Epub 2017 Oct 5.

Department of Biomedical Engineering, Virginia Tech-Wake Forest University, School of Biomedical Engineering and Sciences, Wake Forest School of Medicine , Winston-Salem, North Carolina 27101, United States.

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http://dx.doi.org/10.1021/acs.nanolett.7b03911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704975PMC
November 2017

The SAMHD1 dNTP Triphosphohydrolase Is Controlled by a Redox Switch.

Antioxid Redox Signal 2017 Dec 18;27(16):1317-1331. Epub 2017 Apr 18.

1 Department of Biochemistry, Center for Structural Biology , Wake Forest School of Medicine, Winston-Salem, North Carolina.

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http://dx.doi.org/10.1089/ars.2016.6888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655415PMC
December 2017

The Exonuclease Trex2 Shapes Psoriatic Phenotype.

J Invest Dermatol 2016 12 27;136(12):2345-2355. Epub 2016 Jun 27.

Departament de Patologia i Terapèutica Experimental, Facultat de Medicina, Campus de Bellvitge, Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.jid.2016.05.122DOI Listing
December 2016

The multidimensional nature of antiviral innate immunity.

Cell Host Microbe 2015 Apr;17(4):423-5

Department of Biochemistry, Molecular Biology and Biophysics, Institute for Molecular Virology, Center for Genome Engineering, and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.

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http://dx.doi.org/10.1016/j.chom.2015.03.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616157PMC
April 2015

Exonuclease TREX1 degrades double-stranded DNA to prevent spontaneous lupus-like inflammatory disease.

Proc Natl Acad Sci U S A 2015 Apr 6;112(16):5117-22. Epub 2015 Apr 6.

Department of Biochemistry, Center for Structural Biology, Wake Forest School of Medicine, Winston-Salem, NC 27157

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http://dx.doi.org/10.1073/pnas.1423804112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413332PMC
April 2015

Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding.

Cell Cycle 2015 ;14(4):668-73

a Department of Biochemistry; Center for Structural Biology ; Wake Forest School of Medicine ; Winston-Salem , NC USA.

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https://www.tandfonline.com/doi/full/10.4161/15384101.2014.9
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http://dx.doi.org/10.4161/15384101.2014.994996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615118PMC
December 2015

The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers.

J Biol Chem 2014 Apr 9;289(16):11556-65. Epub 2014 Mar 9.

From the Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157.

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http://dx.doi.org/10.1074/jbc.M114.559252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036290PMC
April 2014

The TREX1 C-terminal region controls cellular localization through ubiquitination.

J Biol Chem 2013 Oct 26;288(40):28881-92. Epub 2013 Aug 26.

From the Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157.

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http://dx.doi.org/10.1074/jbc.M113.503391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789983PMC
October 2013

Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.

DNA Repair (Amst) 2012 Jan 8;11(1):65-73. Epub 2011 Nov 8.

Department of Biochemistry, Center for Structural Biology, Wake Forest University Health Sciences, Winston-Salem, NC 27157, United States.

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http://dx.doi.org/10.1016/j.dnarep.2011.10.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253924PMC
January 2012

Aicardi-Goutieres syndrome gene and HIV-1 restriction factor SAMHD1 is a dGTP-regulated deoxynucleotide triphosphohydrolase.

J Biol Chem 2011 Dec 7;286(51):43596-600. Epub 2011 Nov 7.

Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.C111.317628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243528PMC
December 2011

The TREX1 exonuclease R114H mutation in Aicardi-Goutières syndrome and lupus reveals dimeric structure requirements for DNA degradation activity.

J Biol Chem 2011 Nov 21;286(46):40246-54. Epub 2011 Sep 21.

Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M111.297903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220555PMC
November 2011

Dominant mutation of the TREX1 exonuclease gene in lupus and Aicardi-Goutieres syndrome.

J Biol Chem 2011 Sep 1;286(37):32373-82. Epub 2011 Aug 1.

Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M111.276287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173215PMC
September 2011

Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome.

J Biol Chem 2011 May 16;286(19):16984-91. Epub 2011 Mar 16.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M111.228833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089542PMC
May 2011

Bypass of N²-ethylguanine by human DNA polymerase κ.

DNA Repair (Amst) 2011 Jan 16;10(1):56-64. Epub 2010 Oct 16.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.

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http://dx.doi.org/10.1016/j.dnarep.2010.09.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010520PMC
January 2011

The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction.

J Biol Chem 2010 Feb 18;285(6):3617-24. Epub 2009 Nov 18.

Department of Biochemistry, Center for Structural Biology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA

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http://dx.doi.org/10.1074/jbc.M109.059048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823502PMC
February 2010

DNA binding induces active site conformational change in the human TREX2 3'-exonuclease.

Nucleic Acids Res 2009 Apr 24;37(7):2411-7. Epub 2009 Mar 24.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.

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http://dx.doi.org/10.1093/nar/gkp025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673414PMC
April 2009

A DNA polymerase-{alpha}{middle dot}primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells.

J Biol Chem 2009 Feb 31;284(9):5807-18. Epub 2008 Dec 31.

Department of Medicine and Cancer Center of the University of California, San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/jbc.M807593200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645831PMC
February 2009

RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases.

J Mol Med (Berl) 2009 Jan 26;87(1):25-30. Epub 2008 Nov 26.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, NC, 27157, USA.

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http://dx.doi.org/10.1007/s00109-008-0422-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852111PMC
January 2009

Lesion bypass of N2-ethylguanine by human DNA polymerase iota.

J Biol Chem 2009 Jan 3;284(3):1732-40. Epub 2008 Nov 3.

Department of Biochemistry and Center for Structural Biology, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M807296200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615510PMC
January 2009

The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease.

J Biol Chem 2008 Nov 18;283(46):31649-56. Epub 2008 Sep 18.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M806155200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2581595PMC
November 2008

Cooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonuclease.

J Biol Chem 2008 Aug 5;283(31):21441-52. Epub 2008 Jun 5.

Department of Biochemistry, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.

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http://dx.doi.org/10.1074/jbc.M803629200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2490786PMC
August 2008

A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus.

J Mol Med (Berl) 2007 May 18;85(5):531-7. Epub 2007 Apr 18.

Klinik für Kinder-und Jugendmedizin, Technische Universität Dresden, Fetscherstr. 74, 01307, Dresden, Germany.

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http://dx.doi.org/10.1007/s00109-007-0199-9DOI Listing
May 2007

The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering.

J Biol Chem 2007 Apr 9;282(14):10537-43. Epub 2007 Feb 9.

Department of Biochemistry, Center for Structural Biology, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157.

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http://www.jbc.org/content/282/14/10537.full.pdf
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http://www.jbc.org/cgi/doi/10.1074/jbc.M700039200
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http://dx.doi.org/10.1074/jbc.M700039200DOI Listing
April 2007

WRN exonuclease activity is blocked by DNA termini harboring 3' obstructive groups.

Mech Ageing Dev 2007 Mar 20;128(3):259-66. Epub 2006 Dec 20.

Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States.

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http://dx.doi.org/10.1016/j.mad.2006.12.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1920796PMC
March 2007

Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex.

J Biol Chem 2006 Dec 13;281(50):38466-71. Epub 2006 Sep 13.

Laboratory of Structural Biology, National Institute of Environmental Health Sciences/NIH, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA.

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http://dx.doi.org/10.1074/jbc.M606917200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876720PMC
December 2006

Replication of N2-ethyldeoxyguanosine DNA adducts in the human embryonic kidney cell line 293.

Chem Res Toxicol 2006 Jul;19(7):960-7

Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1021/tx060084aDOI Listing
July 2006

Mutagenesis by exocyclic alkylamino purine adducts in Escherichia coli.

Mutat Res 2006 Jul 20;599(1-2):1-10. Epub 2006 Feb 20.

Wake Forest University Health Sciences, Winston-Salem, NC, USA.

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http://dx.doi.org/10.1016/j.mrfmmm.2005.12.014DOI Listing
July 2006

The human TREX2 3' -> 5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis.

J Biol Chem 2005 Apr 19;280(15):15212-8. Epub 2005 Jan 19.

Department of Biochemistry, Center for Structural Biology, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1074/jbc.M500108200DOI Listing
April 2005

Dysfunctional proofreading in the Escherichia coli DNA polymerase III core.

Biochem J 2004 Dec;384(Pt 2):337-48

Wake Forest University Health Sciences, Department of Biochemistry, Winston-Salem, NC 27157, USA.

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http://dx.doi.org/10.1042/BJ20040660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134117PMC
December 2004

The N2-ethylguanine and the O6-ethyl- and O6-methylguanine lesions in DNA: contrasting responses from the "bypass" DNA polymerase eta and the replicative DNA polymerase alpha.

Chem Res Toxicol 2003 Dec;16(12):1616-23

Department of Biochemistry and Department of Cancer Biology, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.

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http://dx.doi.org/10.1021/tx034164fDOI Listing
December 2003

Elucidation of the epsilon-theta subunit interface of Escherichia coli DNA polymerase III by NMR spectroscopy.

Biochemistry 2003 Apr;42(13):3635-44

Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Box 12233, Research Triangle Park, North Carolina 27709, USA.

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http://dx.doi.org/10.1021/bi0205451DOI Listing
April 2003

Model for the catalytic domain of the proofreading epsilon subunit of Escherichia coli DNA polymerase III based on NMR structural data.

Biochemistry 2002 Jan;41(1):94-110

Laboratory of Structural Biology and Laboratory of Molecular Genetics, NIEHS, Box 12233, Research Triangle Park, North Carolina 27709, USA.

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http://dx.doi.org/10.1021/bi0114170DOI Listing
January 2002