Publications by authors named "Frank Buchholz"

100Publications

The Paf1 complex positively regulates enhancer activity in mouse embryonic stem cells.

Life Sci Alliance 2021 03 29;4(3). Epub 2020 Dec 29.

Medical Systems Biology, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany

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March 2021

MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.

EMBO Rep 2020 Oct 15:e50155. Epub 2020 Oct 15.

National Center for Tumor Diseases (NCT/UCC) Dresden, German Cancer Research Center (DKFZ), University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

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October 2020

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases.

Sci Rep 2020 08 19;10(1):13985. Epub 2020 Aug 19.

Structural Bioinformatics, BIOTEC, TU Dresden, Tatzberg 47-51, 01307, Dresden, Germany.

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August 2020

Comparison of GeneChip, nCounter, and Real-Time PCR-Based Gene Expressions Predicting Locoregional Tumor Control after Primary and Postoperative Radiochemotherapy in Head and Neck Squamous Cell Carcinoma.

J Mol Diagn 2020 06 2;22(6):801-810. Epub 2020 Apr 2.

German Cancer Consortium (DKTK) partner site Dresden, Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg; the OncoRay-National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany; Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; National Center for Tumor Diseases (NCT), Partner Site Dresden, Germany: German Cancer Research Center (DKFZ), Heidelberg, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, and Helmholtz Association/Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

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June 2020

In-cell identification and measurement of RNA-protein interactions.

Nat Commun 2019 11 22;10(1):5317. Epub 2019 Nov 22.

Institut Curie, PSL Research University, CNRS UMR3215, INSERM U934, Paris, 75005, France.

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November 2019

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus.

Nucleic Acids Res 2020 01;48(1):472-485

Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany.

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January 2020

A Tug-of-War between Cell Shape and Polarity Controls Division Orientation to Ensure Robust Patterning in the Mouse Blastocyst.

Dev Cell 2019 12 14;51(5):564-574.e6. Epub 2019 Nov 14.

European Molecular Biology Laboratory (EMBL), Heidelberg, Germany; Institute for Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan. Electronic address:

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December 2019

CRISPR/Cas9 as a tool to dissect cancer mutations.

Methods 2019 07 10;164-165:36-48. Epub 2019 May 10.

Carl Gustav Carus Faculty of Medicine, UCC, Section Medical Systems Biology, TU Dresden, Germany; National Center for Tumor Diseases (NCT), University Hospital Carl Gustav Carus, TU Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg and German Cancer Consortium (DKTK) Partner Site Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. Electronic address:

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July 2019

Comparative RNAi Screens in Isogenic Human Stem Cells Reveal SMARCA4 as a Differential Regulator.

Stem Cell Reports 2019 05 25;12(5):1084-1098. Epub 2019 Apr 25.

Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307 Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg and German Research Consortium (DKTK), Partner Site Dresden, 01307 Dresden, Germany; National Center for Tumor Diseases (NCT), University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Electronic address:

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May 2019

Repeat FMISO-PET imaging weakly correlates with hypoxia-associated gene expressions for locally advanced HNSCC treated by primary radiochemotherapy.

Radiother Oncol 2019 06 9;135:43-50. Epub 2019 Mar 9.

OncoRay - National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden-Rossendorf, Germany; Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany; German Cancer Consortium (DKTK), Partner Site Dresden, Germany; Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany; National Center for Tumor Diseases (NCT), Partner Site Dresden, Germany: German Cancer Research Center (DKFZ), Heidelberg, Germany; Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany, and; Helmholtz Association/Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany; Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiooncology - OncoRay, Germany.

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June 2019

Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesis.

Life Sci Alliance 2019 04 27;2(2). Epub 2019 Feb 27.

CRTD-Center for Regenerative Therapies, School of Medicine, Technische Universität Dresden, Dresden, Germany

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April 2019

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.

Sci Rep 2018 09 27;8(1):14453. Epub 2018 Sep 27.

Stem Cell Engineering, Biotechnology Center, Technische Universität Dresden, 01307, Dresden, Germany.

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September 2018

STK3 is a therapeutic target for a subset of acute myeloid leukemias.

Oncotarget 2018 May 22;9(39):25458-25473. Epub 2018 May 22.

Universitäts KrebsCentrums Dresden, Medical Systems Biology, Medical Faculty, Technische Universität Dresden, Dresden, Germany.

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May 2018

The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells.

PLoS One 2018 24;13(1):e0191682. Epub 2018 Jan 24.

Medical Systems Biology, Faculty of Medicine Carl Gustav Carus, University Cancer Center, TU Dresden, Dresden, Germany.

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March 2018

Transcription factor Runx1 is pro-neurogenic in adult hippocampal precursor cells.

PLoS One 2018 11;13(1):e0190789. Epub 2018 Jan 11.

German Center for Neurodegenerative Diseases (DZNE) Dresden, Dresden, Germany.

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February 2018

Comparison of detection methods for HPV status as a prognostic marker for loco-regional control after radiochemotherapy in patients with HNSCC.

Radiother Oncol 2018 04 30;127(1):27-35. Epub 2017 Dec 30.

German Cancer Research Center (DKFZ), Heidelberg, Germany, and German Cancer Consortium (DKTK), partner site Dresden, Germany; OncoRay - National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany; Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg, , Germany.

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April 2018

lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1.

Cell Rep 2017 Dec;21(11):3012-3021

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg and German Cancer Consortium (DKTK) partner site Dresden, 01307 Dresden, Germany; National Center for Tumor Diseases (NCT), University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany. Electronic address:

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December 2017

MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting.

Mol Cell Proteomics 2018 02 30;17(2):384-396. Epub 2017 Nov 30.

From the ‡Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany;

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February 2018

Development of a genetic sensor that eliminates p53 deficient cells.

Nat Commun 2017 11 13;8(1):1463. Epub 2017 Nov 13.

Medical Faculty and University Hospital Carl Gustav Carus, UCC Section Medical Systems Biology, TU Dresden, 01307, Dresden, Germany.

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November 2017

Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding.

Nat Commun 2017 11 2;8(1):1266. Epub 2017 Nov 2.

Department of Biosystems Science and Engineering (D-BSSE), Eidgenössische Technische Hochschule (ETH) Zurich, Mattenstrasse 26, 4058, Basel, Switzerland.

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November 2017

Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.

Nucleic Acids Res 2017 Sep;45(16):9726-9740

Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.

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September 2017

The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.

Dev Cell 2017 02;40(3):235-247.e7

Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany. Electronic address:

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February 2017

HPV status, cancer stem cell marker expression, hypoxia gene signatures and tumour volume identify good prognosis subgroups in patients with HNSCC after primary radiochemotherapy: A multicentre retrospective study of the German Cancer Consortium Radiation Oncology Group (DKTK-ROG).

Radiother Oncol 2016 12 29;121(3):364-373. Epub 2016 Nov 29.

German Cancer Research Center (DKFZ), Heidelberg and German Cancer Consortium (DKTK), Dresden, Germany; Department of Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany; OncoRay - National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden and Helmholtz-Zentrum Dresden - Rossendorf, Germany; National Center for Tumor Diseases (NCT), Partner Site Dresden, Germany; Helmholtz-Zentrum Dresden - Rossendorf, Institute of Radiooncology, Germany. Electronic address:

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December 2016

Antiviral therapy of persistent viral infection using genome editing.

Curr Opin Virol 2016 10 7;20:85-91. Epub 2016 Oct 7.

Heinrich Pette Institute - Leibniz Institute for Experimental Virology, Martinistrasse 52, D-20251 Hamburg, Germany; German Center for Infection Research (DZIF), Partner Site Hamburg, Germany. Electronic address:

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October 2016

Inactivation of Cancer Mutations Utilizing CRISPR/Cas9.

J Natl Cancer Inst 2017 01 30;109(1). Epub 2016 Aug 30.

Affiliations of author: Medical Systems Biology, Medical Faculty Carl Gustav Carus, University cancer center, Technische Universitat Dresden, Dresden, Germany.

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January 2017

Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering.

Sci Rep 2016 07 22;6:30130. Epub 2016 Jul 22.

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus, TU Dresden, Germany.

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July 2016

Cre Recombinase and Other Tyrosine Recombinases.

Chem Rev 2016 10 10;116(20):12785-12820. Epub 2016 May 10.

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus TU Dresden , 01307 Dresden, Germany.

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October 2016

Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells.

Cell Syst 2015 Aug 26;1(2):141-51. Epub 2015 Aug 26.

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Electronic address:

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August 2015

Dissecting mechanisms of mouse embryonic stem cells heterogeneity through a model-based analysis of transcription factor dynamics.

J R Soc Interface 2016 Apr;13(117)

Institute for Medical Informatics and Biometry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

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April 2016

Low Cancer Stem Cell Marker Expression and Low Hypoxia Identify Good Prognosis Subgroups in HPV(-) HNSCC after Postoperative Radiochemotherapy: A Multicenter Study of the DKTK-ROG.

Clin Cancer Res 2016 06 11;22(11):2639-49. Epub 2016 Jan 11.

German Cancer Research Center (DKFZ), Heidelberg and German Cancer Consortium (DKTK), Dresden, Germany. Department of Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. OncoRay - National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. Helmholtz-Zentrum Dresden - Rossendorf, Institute of Radiooncology, Dresden, Germany. National Center for Tumor Diseases (NCT), University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

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June 2016

Antibody-coupled siRNA as an efficient method for in vivo mRNA knockdown.

Nat Protoc 2016 Jan 3;11(1):22-36. Epub 2015 Dec 3.

Department of Medicine A, Hematology and Oncology, University Hospital Muenster, Muenster, Germany.

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January 2016

RNAi profiling of primary human AML cells identifies ROCK1 as a therapeutic target and nominates fasudil as an antileukemic drug.

Blood 2015 Jun 30;125(24):3760-8. Epub 2015 Apr 30.

Medizinische Systembiologie, Medizinische Fakultät der Technischen Universität, Universitäts KrebsCentrum, Dresden, Germany; German Cancer Consortium (DKTK), Partner Site Dresden, Dresden, Germany; German Cancer Research Center, Heidelberg, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany.

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June 2015

Targeting Human Long Noncoding Transcripts by Endoribonuclease-Prepared siRNAs.

J Biomol Screen 2015 Sep 24;20(8):1018-26. Epub 2015 Apr 24.

Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus, TU Dresden, Dresden, Germany Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

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September 2015

Antibody-mediated delivery of anti-KRAS-siRNA in vivo overcomes therapy resistance in colon cancer.

Clin Cancer Res 2015 Mar 14;21(6):1383-94. Epub 2015 Jan 14.

Department of Medicine A, Hematology/Oncology, University of Muenster, Muenster, Germany. Department of Medicine IV, Hematology and Oncology, University of Halle, Halle, Germany.

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March 2015

The coilin interactome identifies hundreds of small noncoding RNAs that traffic through Cajal bodies.

Mol Cell 2014 Nov 6;56(3):389-99. Epub 2014 Nov 6.

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany; Department of Molecular Biophysics & Biochemistry, Yale University, 333 Cedar Street, New Haven, CT 06520, USA. Electronic address:

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November 2014

Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation.

PLoS One 2014 6;9(11):e111748. Epub 2014 Nov 6.

Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.

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July 2015

Another brick in the wall: RNAi Screens identify new barriers in iPSC reprogramming.

Cell Stem Cell 2014 Aug;15(2):116-8

University Hospital and Medical Faculty Carl Gustav Carus, UCC, Medical Systems Biology, Technische Universität Dresden, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Electronic address:

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August 2014

Interplay of acetyltransferase EP300 and the proteasome system in regulating heat shock transcription factor 1.

Cell 2014 Feb;156(5):975-85

Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Electronic address:

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February 2014

[Revolutionizing HIV-therapy by Tre-Recombinase? Initial observations and current state of development].

MMW Fortschr Med 2013 Jun;155 Suppl 1:22-3

Heinrich-Pette-lnstitut - Leibniz-lnstitut für Experimentelle Virologie, Hamburg.

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June 2013

Quantitative interaction screen of telomeric repeat-containing RNA reveals novel TERRA regulators.

Genome Res 2013 Dec 6;23(12):2149-57. Epub 2013 Aug 6.

Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany;

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December 2013

Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15.

Mol Biol Cell 2013 Aug 3;24(16):2558-69. Epub 2013 Jul 3.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom.

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August 2013

Runx1 is essential at two stages of early murine B-cell development.

Blood 2013 Jul 23;122(3):413-23. Epub 2013 May 23.

Molecular Pathology, Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg, Germany.

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July 2013

Engineered DNA modifying enzymes: components of a future strategy to cure HIV/AIDS.

Antiviral Res 2013 Feb 23;97(2):211-7. Epub 2012 Dec 23.

University Carl Gustav Carus and Medical Faculty, TU Dresden, Germany.

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February 2013

Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system.

Nucleic Acids Res 2013 Jan 9;41(2):e37. Epub 2012 Nov 9.

Medical Systems Biology, Medical Faculty and University Hospital Carl Gustav Carus, University of Technology, Fetscherstrasse 74, 01307 Dresden, Germany.

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January 2013

Designing efficient and specific endoribonuclease-prepared siRNAs.

Methods Mol Biol 2013 ;942:193-204

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

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February 2013

Combined RNAi and localization for functionally dissecting long noncoding RNAs.

Nat Methods 2012 Feb 12;9(4):360-2. Epub 2012 Feb 12.

University of Technology Dresden, University Hospital and Medical Faculty Carl Gustav Carus, Department of Medical Systems Biology, Dresden, Germany.

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February 2012

A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.

Nat Cell Biol 2011 Jun 5;13(7):809-18. Epub 2011 Jun 5.

University of Technology Dresden, University Hospital and Medical Faculty Carl Gustav Carus, Department of Medical Systems Biology, Fetscherstraße 74, D-01307 Dresden, Germany.

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June 2011

From RNAi screens to molecular function in embryonic stem cells.

Stem Cell Rev Rep 2012 Mar;8(1):32-42

University Hospital Carl Gustav Carus and Medical Faculty, University of Technology Dresden, Fetscherstr. 74, 01307, Dresden, Germany.

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March 2012

High-throughput RNAi screening in mammalian cells with esiRNAs.

Methods 2011 Apr 23;53(4):424-9. Epub 2010 Dec 23.

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany.

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April 2011

In vitro evolution and analysis of HIV-1 LTR-specific recombinases.

Methods 2011 Jan 26;53(1):102-9. Epub 2010 Jun 26.

Max-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

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January 2011

SeLOX--a locus of recombination site search tool for the detection and directed evolution of site-specific recombination systems.

Nucleic Acids Res 2010 Jul 6;38(Web Server issue):W293-8. Epub 2010 Jun 6.

Max Planck Institute for the Molecular Cell Biology and Genetics, Dresden, Germany.

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July 2010

PhenoFam-gene set enrichment analysis through protein structural information.

BMC Bioinformatics 2010 May 17;11:254. Epub 2010 May 17.

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr, 108, 01307 Dresden, Germany.

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May 2010

MISSION esiRNA for RNAi screening in mammalian cells.

J Vis Exp 2010 May 12(39). Epub 2010 May 12.

Max Planck Institute of Molecular Cell Biology and Genetics.

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May 2010

A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth.

EMBO Rep 2010 Apr 5;11(4):305-11. Epub 2010 Feb 5.

Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany.

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April 2010

Engineering DNA processing enzymes for the postgenomic era.

Authors:
Frank Buchholz

Curr Opin Biotechnol 2009 Aug 21;20(4):383-9. Epub 2009 Aug 21.

Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden D-01307, Germany.

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August 2009

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.

Dis Model Mech 2009 Sep-Oct;2(9-10):508-15. Epub 2009 Aug 19.

Center for Regenerative Therapies Dresden, BioInnovationsZentrum Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany.

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January 2010

Molecular evolution of the tre recombinase.

Authors:
Frank Buchholz

J Vis Exp 2008 May 29(15). Epub 2008 May 29.

Max Plank Institute for Molecular Cell Biology and Genetics, Dresden.

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May 2008

Systems biology of mammalian cell division.

Cell Cycle 2008 Jul 21;7(14):2123-8. Epub 2008 May 21.

Department of Human Genetics and Institute of Genomics and Systems Biology, The University of Chicago, Chicago, Illinois, USA.

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July 2008

HIV-1 proviral DNA excision using an evolved recombinase.

Science 2007 Jun;316(5833):1912-5

Max-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany.

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June 2007

Tandem affinity purification of functional TAP-tagged proteins from human cells.

Nat Protoc 2007 ;2(5):1145-51

Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 1, 1030 Vienna, Austria.

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January 2008