Publications by authors named "Francesco Esposito"

173 Publications

Critical role of the high mobility group A proteins in hematological malignancies.

Hematol Oncol 2021 Oct 12. Epub 2021 Oct 12.

Department of Molecular Medicine and Medical Biotechnology (DMMBM), National Research Council (CNR), Institute for Experimental Endocrinology and Oncology (IEOS) "G. Salvatore", University of Naples "Federico II", Naples, Italy.

The high mobility group A (HMGA) protein family is composed of three non-histone chromatin remodeling proteins that act as architectural transcriptional factors. Indeed, although HMGA proteins lack transcriptional activity per se, they bind the minor groove of DNA at AT-rich sequences, and, interacting with the transcription machinery, are able to modify chromatin modeling, thus regulating the expression of several genes. HMGA proteins have been deeply involved in embryogenesis process, and a large volume of studies has pointed out their key role in human cancer. Here, we review the studies on the role of the HMGA proteins in human hematological malignancies: they are overexpressed in most of the cases and their expression correlates with a reduced survival. In some cases, such as in acute lymphoblastic leukemia and acute myelogenous leukemia, HMGA2 gene rearrangements have been also described. Finally, recent studies evidence a synergism between HMGA and EZH2 in diffuse B-cell lymphomas, suggesting an innovative therapy for this disease based on the inhibition of the function of both these proteins.
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http://dx.doi.org/10.1002/hon.2934DOI Listing
October 2021

The experience of patients with diabetes with the use of telemedicine and teleassistance services during the COVID-19 pandemic in Italy: Factors associated with perceived quality and willingness to continue.

Diabetes Res Clin Pract 2021 Sep 14;180:109047. Epub 2021 Sep 14.

AUSL Diabetes Unit Romagna, Ravenna, Italy.

Aim: The purpose of this study is to investigate the individual and contextual determinants of the perceived quality (PQ) of the telemedicine and teleassistance (TMTA) services and the willingness to continue (WC) with them among patients with diabetes using TMTA services during the COVID-19 pandemic in one large region of Italy.

Methods: A structured survey was administered to patients with type 1 and 2 diabetes who used TMTA during the first wave of the COVID-19 pandemic. The questionnaire contained questions on TMTA service experience and participants' socio-demographic characteristics. Multiple regression models investigated the independent factors associated with PQ and WC.

Results: The final analysis included 569 patients with diabetes (54.7% female), with an average age of 58.1 years. TMTA services' PQ and WC were high. A higher education and being unemployed were factors associated with an increased WC. Older age was negatively related to PQ. Perceived support from TMTA service was positively associated with PQ and WC. Perceived increase in disease self-management was positively associated with PQ and WC.

Conclusions: Our study identified several determinants of PQ and WC. These socio-demographic and TMTA-related factors should be considered in the implementation of care pathways integrating in-person visits with TMTA.
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http://dx.doi.org/10.1016/j.diabres.2021.109047DOI Listing
September 2021

Midsternal bump: an infrequent localization of dermoid cysts.

J Ultrasound 2021 Sep 12. Epub 2021 Sep 12.

Department of Radiology, "AORN Santobono Pausilipon", Pediatric Hospital, Naples, Italy.

A 5-month-old boy was evaluated for an unusually large presternal bump present since birth. The ultrasound examination revealed a well-defined soft tissue mass with an oval shape. The lesion demonstrated a regular and well-demarcated outline, with an upper margin that was thinned and inserted into the upper skin plane; the content was anechoic with a small echogenic formation, mobile with changes in the patient's decubitus. The histologic diagnosis was dermoid cyst. Although dermoid cysts are commonly seen in the midline, the midsternal location, found in our patient, is rare. Dermoid cysts can have ultrasonographic features similar to those of other subcutaneous cystic masses. However, if an anechoic cyst with an internal well-circumscribed echogenic ball-like formation is seen within the presternal subcutaneous fat layer, as in our patient, dermoid cyst should be considered in the differential diagnosis of subcutaneous cystic masses.
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http://dx.doi.org/10.1007/s40477-021-00607-6DOI Listing
September 2021

Impact of Anthropic Activities on Soil Quality under Different Land Uses.

Int J Environ Res Public Health 2021 08 10;18(16). Epub 2021 Aug 10.

Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Via Cinthia, 80126 Napoli, Italy.

Anthropization often leads to land use transformation, causing deep changes to soil properties and its quality. Land use change could be an environmental and socioeconomic problem, as it impacts soil quality and ecosystem services. There is an urgent need to understand the pressures affecting soil quality. The aim of the work is to quantify the impact of different land uses on soil abiotic and biotic properties and on its quality. To achieve the aims, soils from different land uses (forest, urban and agricultural) were collected in the surroundings of Naples and analyzed for pH, water content, contents of C and N, C/N ratio and total and available concentrations of Cu, Ni and Pb, microbial and fungal biomasses, basal respiration and metabolic quotient. Then, a soil quality index (SQI) was calculated for each land use. The results showed that soil abiotic and biotic properties of the agricultural sites differed from those of forest and urban sites. At agricultural sites, microbial abundances decreased due to low amount of C and N and to high amount of Cu and Pb. This caused low use efficiency of energetic substrates and a reduced soil quality of agricultural sites as compared to forest and urban sites.
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http://dx.doi.org/10.3390/ijerph18168423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393834PMC
August 2021

Type II pleuropulmonary blastoma in a 3-years-old female with dyspnea: a case report and review of literature.

Radiol Case Rep 2021 Sep 18;16(9):2736-2741. Epub 2021 Jul 18.

Luigi Vanvitelli University, Naples, Italy.

Pleuropulmonary blastoma (PPB) is a rare but aggressive pediatric tumor originates from either lung or pleura. It was recently linked to the DICER I mutation as a part of predisposition syndrome for different type of tumor. It is characterized histologically by a primitive, variably mixed blastomatous and sarcomatous tissue. PPB is classified into four subtypes: cystic (type I and type Ir); cystic and solid (type II); solid (type III). PPB has no characteristic imaging findings. Integrated imaging can help to make a differential diagnosis and to recognize the subtypes in order to set up therapy. An early recognition and differentiation from congenital airway malformations and other benign cysts are very important. The treatment consists in a multimodal therapy including surgery and chemoterapy. We report a case of 3 years old female admitted at our hospital with fever, non productive cough and dyspnea, who was diagnosed with type II PPB.
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http://dx.doi.org/10.1016/j.radcr.2021.06.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319522PMC
September 2021

Role of ultrasound in diagnosis of neonatal breast enlargement: a newborn case report.

Radiol Case Rep 2021 Sep 15;16(9):2692-2696. Epub 2021 Jul 15.

U.O. Emergency Radiology, A.O.R.N. Santobono-Pausilipon Children Hospital, Via Mario Fiore, Naples, Italy.

Neonatal breast enlargement is a hormone-related condition, mostly asymptomatic\physiological, with a well-recognizable sonographic appearance but limited data in the literature. It can be uni-or bilateral. Typically described in the first week of life, the transient lesion disappears spontaneously within 6 months. The main differential diagnosis is neonatal mastitis, a breast tissue infection that requires a specific antibiotic therapy. Knowledge of clinical and imaging findings is crucial, and ultrasound represents a reliable tool which allows a quick identification and an excellent examination of neonatal breast abnormalities. We aim to highlight sonographic classic features of benign neonatal breast enlargement showing how ultrasound rules out real breast masses helping to differentiate between it and mastitis when clinical and laboratory data are inconclusive. We describe a 2-day-old male with bilateral breast swelling more pronounced on the right side.
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http://dx.doi.org/10.1016/j.radcr.2021.06.057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318999PMC
September 2021

Exploring the Gap Between Excess Mortality and COVID-19 Deaths in 67 Countries.

JAMA Netw Open 2021 07 1;4(7):e2117359. Epub 2021 Jul 1.

Department of Biomedical and Neuromotor Science, Alma Mater Studiorum-University of Bologna, Bologna, Italy.

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http://dx.doi.org/10.1001/jamanetworkopen.2021.17359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285734PMC
July 2021

Publisher Correction: HMGA1-pseudogene7 transgenic mice develop B cell lymphomas.

Sci Rep 2021 Jun 11;11(1):12741. Epub 2021 Jun 11.

Istituto di Endocrinologia ed Oncologia Sperimentale - CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli "Federico II", Naples, Italy.

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http://dx.doi.org/10.1038/s41598-021-92173-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196011PMC
June 2021

HMGA1 induces EZH2 overexpression in human B-cell lymphomas.

Am J Cancer Res 2021 15;11(5):2174-2187. Epub 2021 May 15.

Institute of Endocrinology and Experimental Oncology-CNR c/o Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II" Naples, Italy.

EZH2 is an enzymatic subunit of PRC2, an epigenetic regulator that triggers the methylation of the histone H3 lysine 27 silencing the transcription of several genes. EZH2 has a critical role in cancer progression, since its overexpression has been associated with increased cancer cell invasiveness, drug resistance and poor patient survival. However, the mechanisms accounting for EZH2 overexpression in cancer remain still unclear. Intriguingly, also HMGA protein overexpression is a feature of many human malignancies and correlates with the presence of metastases and a poor outcome. The HMGA proteins, including HMGA1 and HMGA2, belong to the architectural transcription factors that play a key role in the organization of chromatin structure. Here, we report a statistically significant correlation between HMGA1 and EZH2 expression in human lymphomas. We demonstrate that HMGA1 is able to bind promoter and induce its activity. Consistently, silencing of expression results in the downregulation of the levels leading to a decreased proliferation and migration rate of human lymphoma cell lines. Therefore, these data identify HMGA1 as an activator, suggesting a novel molecular mechanism contributing to EZH2 overexpression in human malignancies and a synergism of these proteins in cancer progression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167683PMC
May 2021

Foreign body ingestion in children: Beware of disk batteries and multiple magnets.

Acta Paediatr 2021 Oct 8;110(10):2862-2864. Epub 2021 Jun 8.

NESMOS Department, Chair of Pediatrics, Faculty of Medicine and Psychology, Sapienza University of Rome, Sant'Andrea University Hospital, Rome, Italy.

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http://dx.doi.org/10.1111/apa.15957DOI Listing
October 2021

The Determinants of Health-Related Quality of Life in a Sample of Primary School Children: A Cross-Sectional Analysis.

Int J Environ Res Public Health 2021 03 21;18(6). Epub 2021 Mar 21.

Department of Biomedical and Neuromotor Science, University of Bologna, 40126 Bologna, Italy.

Background: Health-related quality of life (HRQoL) in childhood is a multidimensional construct with many sub dimensions of subjective experience, including physical activity (PA), psychological well-being, social interaction, and school performance, that represents a fundamental health outcome to assess a child's physical and psycho-social functioning. Our study aims to explore the potential predictors of children's health-related quality of life, using a convenience sample from the Imola Active Break Study (I-MOVE), considering demographic, anthropometric measures, PA level measured by Actigraph accelerometers, parent-reported/self-reported HRQoL, and body image.

Methods: A cross-sectional analysis was conducted among 151 primary school children in Italy. HRQoL was assessed using the Italian version 4.0 of the Paediatric Quality of Life (PedsQL) questionnaire.

Results: Children who spent more time partaking in moderate PA were associated with a higher total PedsQL score ( < 0.03). Mother's body mass index (BMI) was the only variable statistically significant associated with the physical health domain of PedsQL. Parent's proxy-report perception concerning children's psychosocial health was statistically relevant. The children's gender, age, and BMI had no association with any of the HRQoL outcomes.

Discussion: Parent proxy-report psychosocial health and mother's BMI should be considered as predictors of HRQoL for the psychosocial and physical domain. PA should be implemented in order to improve the HRQoL of primary school children.
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http://dx.doi.org/10.3390/ijerph18063251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004180PMC
March 2021

Isolated intestinal Ganglioneuromatosis: case report and literature review.

Ital J Pediatr 2021 Mar 30;47(1):80. Epub 2021 Mar 30.

NESMOS Department, Faculty of Medicine and Psychology, Sapienza University of Rome, Pediatric Unit, Sant'Andrea University Hospital, Rome, Italy.

Background: Intestinal Ganglioneuromatosis (IG) is a rare disorder of the enteric nervous system. In pediatric age it is often associated with genetic syndromes such as Neurofibromatosis 1 (NF1), multiple endocrine neoplasia type 2B (MEN2B) and Cowden syndrome (PTEN mutation), and ganglioneuromas (GNs) may be sometimes the first sign of the disease. Isolated GNs are rare and sporadic. Clinical symptom vary and depend on the size and on the location of the GNs. This disorder affects intestinal motility and it, consequently, causes changes in bowel habits, abdominal pain, occlusive symptoms and rarely lower gastrointestinal bleeding secondary to ulceration of the intestinal mucosa. On the other hand, patients can remain asymptomatic for many years.

Case Presentation: We describe a 9-year-old boy referred to our emergency department for right lower quadrant abdominal pain. No familial history for gastrointestinal disorders. No history of fever or weight loss. At physical examination, he had diffused abdominal pain. Abdominal ultrasonography showed a hypoechoic formation measuring 41.8 mm by 35 mm in the right lower quadrant of the abdomen. Routine blood tests were normal, but fecal occult blood test was positive. Abdominal TC confirmed the hypodense formation, of about 5 cm in transverse diameter, in the right hypochondrium that apparently invaginated in the caecum-last ileal loop. Colonoscopy showed in the cecum an invaginated polypoid lesion of the terminal ileal loop. Laparoscopic resection of the polypoid lesion was performed. Histological diagnosis of the large neoplasm observed in the terminal ileum was diffuse ganglioneuromatosis. NF1, RET and PTEN gene tests resulted negative for specific mutations. At the 1 year follow-up, the patient presented good general condition and blood tests, fecal occult blood test, esophagogastroduodenoscopy, colonoscopy and MR-enterography were negative.

Conclusions: Only few cases are reported in literature of IG in pediatric age. Although rare, the present case suggests that this disorder must be taken in consideration in every patient with GI symptoms such as abdominal pain, constipation, lower intestinal bleeding, in order to avoid a delayed diagnosis.
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http://dx.doi.org/10.1186/s13052-021-01024-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008650PMC
March 2021

MPPED2 is downregulated in glioblastoma, and its restoration inhibits proliferation and increases the sensitivity to temozolomide of glioblastoma cells.

Cell Cycle 2021 04 18;20(7):716-729. Epub 2021 Mar 18.

Institute for Experimental Endocrinology and Oncology (IEOS) "G. Salvatore", National Research Council (CNR), Naples, Italy.

Glioblastoma (GBM) is the most aggressive and lethal neoplasia of the central nervous system in adults. Based on the molecular signature genes, GBM has been classified in proneural, neural, mesenchymal and classical subtypes. The Metallophosphoesterase-domain-containing protein 2 () gene encodes a metallophosphodiesterase protein highly conserved throughout the evolution. MPPED2 downregulation, likely due to its promoter hypermethylation, has been found in several malignant neoplasias and correlated with a poor prognosis. In this study, we aimed to investigate the expression and the functional role of MPPED2 in GBM. TCGA and Gravendeel databases were employed to explore the expression levels in this type of tumor. We have found that expression is downregulated in GBM patients, showing a positive correlation with survival. Moreover, TCGA and Gravendeel data also revealed that expression negatively correlates with the most aggressive mesenchymal subtype. Additionally, the restoration of expression in U251 and GLI36 GBM cell lines decreases cell growth, migration and enhanced the sensitivity to the temozolomide, inducing apoptotic cell death, of GBM cells. These findings suggest that the restoration of MPPED2 function can be taken into consideration for an innovative GBM therapy.
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http://dx.doi.org/10.1080/15384101.2021.1901042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078659PMC
April 2021

Laparoscopic hybrid pancreaticoduodenectomy: Initial single center experience.

Ann Hepatobiliary Pancreat Surg 2021 Feb;25(1):102-111

Department of Hepatobiliary, Pancreatic and Digestive Surgery, University Hospital Robert Debré of Reims, University of Champagne-Ardenne, Reims, France.

Backgrounds/aims: Pancreaticoduodenectomy (PD) is the gold standard for the treatment of periampullary tumors. Many specialized centers have adopted the totally laparoscopic or hybrid laparoscopic PD (LPD). However, this procedure has not yet been standardized and serious debate is taking place towards its safety and feasibility. Herein, we report our recent experience whit hybrid-LPD.

Methods: During 2019 in our department 56 PD were performed and 21 (37.5%) underwent hybrid-LPD. We have retrospectively reviewed the short-term outcomes of these patients.

Results: Main indication was pancreatic adenocarcinoma (71,4%). The median operative time and intraoperative blood loss were respectively 425 min (range, 226 to 576) and 317 ml (range 60 to 800 ml). Conversion to an open procedure was required in 4 patients (19%): 2 with suspected vein involvement, 1 for mesenteric panniculitis and 1 for biliary injury. The post-operative complication rate was 42.8% (9/21). Regarding post-operative pancreatic fistula, three patients (14.2%) had grade B and 1 grade C (4.7%). Median length of hospital stay was 14 days (range 9-23) and 90- days mortality was 4.7%. The mean number of harvested lymph nodes was 17.7 (range 12 to 26). The rate of margins R0 was 80%; R1 >0<1 mm was 10.5% and R1 0 mm was 9.5%.

Conclusions: Hydrid-LPD is safe and feasible. Careful patient selection and increasing experience can reduce the risk of post-operative complications.
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http://dx.doi.org/10.14701/ahbps.2021.25.1.102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952661PMC
February 2021

nephron-sparing surgery and kidney autotransplantation for renal tumors.

J Surg Case Rep 2021 Feb 16;2021(2):rjab004. Epub 2021 Feb 16.

Department of Digestive and Oncological Surgery, Grand Hopital de l'Est Francilien, Jossigny, France.

The nephron-sparing techniques allow the excision of kidney tumors preserving renal function and ensuring adequate oncological results. We describe a case of a 77-year-old patient who underwent an partial nephrectomy with orthotopic autotransplantation for kidney cancer. The postoperative course was marked by bleeding which required radiological embolization. Postoperative dialysis was required for about 1 month. The anatomopathological examination showed a clear cell carcinoma staged pT1b, pNX, R0. At 2 years follow-up, no recurrence was detected with a complete renal function restoration. Our experience shows that nephron-sparing surgery with autotransplantation is a good alternative to total nephrectomy in the case of voluminous or perihilar tumors. Considering the high morbidity of this procedure, it should be only performed in specialized centers.
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http://dx.doi.org/10.1093/jscr/rjab004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890789PMC
February 2021

miRNAs and Biomarkers in Testicular Germ Cell Tumors: An Update.

Int J Mol Sci 2021 Jan 30;22(3). Epub 2021 Jan 30.

Istituto di Endocrinologia ed Oncologia Sperimentale-CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli "Federico II", Napoli 80131, Italy.

Testicular germ cell tumors (TGCTs) are the leading form of solid cancer and death affecting males between the ages of 20 and 40. Today, their surgical resection and chemotherapy are the treatments of first choice, even if sometimes this is not enough to save the lives of patients with TGCT. As seen for several tumors, the deregulation of microRNAs (miRNAs) is also a key feature in TGCTs. miRNAs are small molecules of RNA with biological activity that are released into biological fluids by testicular cancer cells. Their presence, therefore, can be detected and monitored by considering miRNAs as diagnostic and prognostic markers for TGCTs. The purpose of this review is to collect all the studies executed on miRNAs that have a potential role as biomarkers for testicular tumors.
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http://dx.doi.org/10.3390/ijms22031380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866514PMC
January 2021

The "Reverse Air-Leak Test" : A New Technique for the Assessment of Low Colorectal Anastomosis.

Ann Coloproctol 2020 Dec 4. Epub 2020 Dec 4.

Department of Coloproctology and Pelvic Disease, St Elisabeth Hospital, Vienna, Austria.

Background: Anastomotic leakage is a fearsome complication in rectal surgery. Surgeons perform the classic air-leak test, although its real effectiveness is still debated. The aim of this study was to describe a personal technique of reverse air leak test in which low colorectal anastomosis were assessed transanally through the intra-rectal irrigation of a few mL of saline solution.

Methods: From October 2014 to November 2019 eleven patients with low rectal cancer (Type 1 in Roullier classification) were included in this study. At the beginning of the procedure, a circular anal dilator (CAD) was inserted into the anus. A side-to-end colorectal anastomosis was performed. A few mL of saline solution were injected into the rectum and the entire anastomotic line was directly explored. The appearance of bubbles was considered as an anastomotic defect and repaired with an interrupted suture. A fluorescence angiography after intravenous injection of Indocyanine green was performed in order to evaluate the perfusion of the anastomosis.

Results: In 4 cases (36.3%) the reverse air-leak test was positive. The defect was repaired and a confirmation test was performed. In all patients, near-infrared evaluation showed not perfusion defect (grade 0) in low colorectal anastomosis. No post-operative fistula was detected in cohort study. A protective stoma was performed in 10 patients. At 90-days there were no complications and stoma closure was performed as planned.

Conclusion: The reverse air-leak test is a simple, feasible and effective procedure to identify anastomotic leak in low colorectal anastomoses.
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http://dx.doi.org/10.3393/ac.2020.09.21.1DOI Listing
December 2020

Congenital Giant Juvenile Xanthogranuloma in a 3-Month-Old Boy.

J Pediatr 2021 04 14;231:287-288. Epub 2020 Dec 14.

Department of Radiology, AORN Santobono Pausilipon, Pediatric Hospital, Naples, Italy.

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http://dx.doi.org/10.1016/j.jpeds.2020.12.013DOI Listing
April 2021

Systemic Cat-Scratch Disease: a "Troublesome" Diagnosis.

Pediatr Infect Dis J 2021 03;40(3):e117-e119

Department of Pediatrics, Santobono-Pausilipon Children's Hospital, Naples, Italy.

Diagnosis of systemic cat scratch disease may be challenging. Here, we describe a case of an immunocompetent girl exhibiting fever and multifocal hepatosplenic abscesses. Diagnostic tests for Bartonella henselae infection (enzyme immunoassay and polymerase chain reaction) were found steadily negative and the diagnosis, suspected on the basis of the Margilet's criteria, was finally confirmed by indirect immunofluorescent antibodies.
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http://dx.doi.org/10.1097/INF.0000000000002996DOI Listing
March 2021

Omental Infarction: An Underrecognized Cause of Right-Sided Acute Abdominal Pain in Children.

Pediatr Emerg Care 2020 Jul 6. Epub 2020 Jul 6.

From the *NESMOS Department, School of Medicine and Psychology, Sapienza University of Rome, Sant'Andrea University Hospital, Rome †General and Emergency Radiology Unit, Antonio Cardarelli Hospital ‡Pediatric Surgery §Pediatric Gastroenterology Units, Santobono-Pausilipon Children Hospital, Naples ∥General Surgery Unit, Sapienza University of Rome, Sant'Andrea University Hospital, Rome ¶Paediatric Radiology Unit, Federico II University Hospital #Radiology Unit, Santobono-Pausilipon Children Hospital, Naples, Italy.

Objective: To evaluate the prevalence of omental infarction (OI) in children with suspected appendicitis, the role of ultrasonography (US) in its diagnosis and management and the efficacy of conservative management.

Methods: Consecutive children with suspected acute appendicitis were prospectively enrolled. Ultrasonography was performed at baseline, during follow-up, before the discharge, and at 15-day intervals until US findings of OI disappeared. All children with a diagnosis of OI were treated conservatively.

Results: One hundred ninety-nine children (91 male; age range, 3-15 years) were evaluated. Eighty-four patients had acute appendicitis. Omental infarctions were found in 14 children (8 male; mean age, 9.8 ± 2.6 years), with an incidence of 7%. Ultrasonography depicted an echogenic mass consistent with OI in all children. Ultrasonography detected in 8 patients a normal-looking appendix, whereas in other 6 patients, it identified neither appendix nor indirect signs of acute appendicitis. A normal appendix has been detected by US during follow-up in 2 of these 6 patients. During follow-up, US finding of OI disappeared in all cases and no signs of acute appendicitis or other disease occurred. All 14 OIs were treated conservatively, with no reported complications.

Conclusions: Omental infarction is an underestimated cause of abdominal pain in children accounting for 7% of patients with suspected appendicitis. Ultrasonography is a useful method for the diagnoses and to guide clinical management of OI. Conservative therapy is a safe option for the management of OI.
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http://dx.doi.org/10.1097/PEC.0000000000002114DOI Listing
July 2020

The shows oncogenic activity .

Cell Cycle 2020 11 10;19(22):2955-2959. Epub 2020 Oct 10.

Istituto di Endocrinologia ed Oncologia Sperimentale - CNR C/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Degli Studi di Napoli "Federico II" , Naples, Italy.

We have recently reported that transgenic mice overexpressing the develop hematological neoplasia marked by monoclonal B-cell populations, and diagnosed as Diffuse Large B-cell Lymphoma. These findings prove the oncogenic role .
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http://dx.doi.org/10.1080/15384101.2020.1829825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714516PMC
November 2020

Recommendations for early diagnosis of Developmental Dysplasia of the Hip (DDH): working group intersociety consensus document.

Ital J Pediatr 2020 Oct 9;46(1):150. Epub 2020 Oct 9.

Radiology Services, Bolzano Health District, Bolzano, Italy.

This consensus document has been prepared by a multidisciplinary group of experts (Paediatricians, Radiologists, Paediatric Orthopaedics) and it is mainly aimed at paediatricians, hospitals and primary care providers. We provide recommendations for the early diagnosis and treatment of Developmental Dysplasia of the Hip (DDH) and indications on its management.
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http://dx.doi.org/10.1186/s13052-020-00908-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547432PMC
October 2020

Occurrence of Acrylamide in Italian Baked Products and Dietary Exposure Assessment.

Molecules 2020 Sep 11;25(18). Epub 2020 Sep 11.

Department of Agricultural Sciences, University of Naples "Federico II", via Università, 100-80055 Portici, Naples, Italy.

Acrylamide (AA) is a neo-formed toxic compound that develops in foods during cooking at temperatures above 120 °C. AA shows in vivo neurotoxic and carcinogenic effects, and it is potentially carcinogenic for humans. Its occurrence is common in baked food, such as bread and similar products. This study set out to analyze bread and sweets from the Italian market to evaluate the effects of the benchmark thresholds set by EU Regulation 2017/2158 and to ascertain the exposure of the Italian population to AA, across three age groups, through the consumption of baked products, according to the margin of exposure (MOE) approach. Two hundred samples were tested, and the content of AA ranged from 31 to 454 µg/kg for bread and products thereof and from 204 to 400 µg/kg for the sweets category. The exposure data did not show any neurotoxic health concern, whereas the MOE related to the carcinogenic endpoint is well below the minimum safety value of 10,000.
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http://dx.doi.org/10.3390/molecules25184156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571032PMC
September 2020

An update on microRNAs as potential novel therapeutic targets in testicular germ cell tumors.

Intractable Rare Dis Res 2020 Aug;9(3):184-186

Dipartimento di Psicologia, Università della Campania "Luigi Vanvitelli", Caserta, Italy.

Testicular germ cell tumors (TGCTs) are the most frequent solid malignant tumors in men 20- 40 years of age and the most frequent cause of death from solid tumors in this age group. Recent studies have underscored the fact that miRNA deregulation is a feature of carcinogenesis, including TGCT development and progression. MiRNAs are a group of small noncoding RNAs that bind to the 3'-untranslated region (UTR) of the targeted mRNAs, thus causing mRNA degradation or the inhibition of its translation, regulating gene expression in a temporal and tissue-specific manner. However, few miRNAs have been found to play key roles in TGCTs; recently, other miRNAs have been identified, representing novel potential therapeutic targets.
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http://dx.doi.org/10.5582/irdr.2020.03025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441029PMC
August 2020

Characterization of transgenic mouse embryonic fibroblasts.

Cell Cycle 2020 09 13;19(18):2281-2285. Epub 2020 Aug 13.

Istituto di Endocrinologia Ed Oncologia Sperimentale del CNR C/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Degli Studi Di Napoli "Federico II" , Naples, Italy.

Latest studies have shown that deregulated pseudogene transcripts contribute to cancer working as competing endogenous RNAs. Our research group has recently demonstrated that the overexpression of two pseudogenes, and , has a critical role in cancer progression. These pseudogenes work sustaining the expression of HMGA1 and other cancer-related genes. We generated a mouse model overexpressing to better study the -pseudogene function in a more physiological context. Here, we show the proliferation rate and the susceptibility to senescence of mouse embryonic fibroblasts obtained from -overexpressing mice to better characterize the HMGA1-pseudogene function. Indeed, our study reports that mouse embryonic fibroblasts (MEFs) derived from mice express higher HMGA1 mRNA and protein levels. Moreover, these cells grow faster and senesce later than wild-type sustaining the oncogenic role of ceRNA crosstalk mediated by .
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http://dx.doi.org/10.1080/15384101.2020.1807080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513866PMC
September 2020

De Novo Transcriptome Assembly and Gene Expression Profiling of the Copepod Feeding on the PUA-Producing Diatom .

Mar Drugs 2020 Jul 27;18(8). Epub 2020 Jul 27.

Integrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy.

Diatoms are the dominant component of the marine phytoplankton. Several diatoms produce secondary metabolites, namely oxylipins, with teratogenic effects on their main predators, crustacean copepods. Our study reports the de novo assembled transcriptome of the calanoid copepod feeding on the oxylipin-producing diatom . Differential expression analysis was also performed between copepod females exposed to the diatom and the control flagellate , which does not produce oxylipins. Our results showed that transcripts involved in carbohydrate, amino acid, folate and methionine metabolism, embryogenesis, and response to stimulus were differentially expressed in the two conditions. Expression of 27 selected genes belonging to these functional categories was also analyzed by RT-qPCR in females exposed to a mixed solution of the oxylipins heptadienal and octadienal at the concentration of 10 µM, 15 µM, and 20 µM. The results confirmed differential expression analysis, with up-regulation of genes involved in stress response and down-regulation of genes associated with folate and methionine metabolism, embryogenesis, and signaling. Overall, we offer new insights on the mechanism of action of oxylipins on maternally-induced embryo abnormality. Our results may also help identify biomarker genes associated with diatom-related reproductive failure in the natural copepod population at sea.
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http://dx.doi.org/10.3390/md18080392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460014PMC
July 2020

Coupling feeding activity, growth rates and molecular data shows dietetic needs of Ciona robusta (Ascidiacea, Phlebobranchia) in automatic culture plants.

Sci Rep 2020 07 9;10(1):11295. Epub 2020 Jul 9.

Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy.

The sea squirt Ciona robusta is a model organism characterized by a transparent body, exhibiting peculiar physiologic and evolutionary characters. In vitro fertilization and breeding of sea squirts is possible, in order to preserve consistent genetic pools. However, some aspects of its biology, as the feeding efficiency according to diet quantity and quality, are still scarcely known. Here we test the effects of three experimental diets on survival and growth, to detect physiological and molecular responses to various types of alimentary suspended particles and the effects of feed concentrations. We also aimed at determining rearing conditions able to limit handling operations, save artificial seawater and control water pollution. Molecular analyses of growth-related genes were performed to detect stressful effects due to feed quality and quantity. A strong effect of doses was highlighted, but water pollution may represent a major concern. A compound diet containing both live algae and non-live particles of a correct size is indispensable to assure development, low stress and high survival rates. Overall, our findings suggest protocols for an easier rearing of Ciona robusta in the laboratory, increasing the potentialities of these organisms as models for research.
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http://dx.doi.org/10.1038/s41598-020-68031-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347631PMC
July 2020

Scurvy in childhood: don't forget it.

Minerva Pediatr 2020 Jul 7. Epub 2020 Jul 7.

Department of Paediatrics AORN "Santobono-Pausilipon", Naples, Italy.

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http://dx.doi.org/10.23736/S0026-4946.20.05586-3DOI Listing
July 2020

Not only fat: omental infarction and its mimics in children. Clinical and ultrasound findings: a pictorial review.

J Ultrasound 2020 Dec 4;23(4):621-629. Epub 2020 Jul 4.

Chair of Pediatrics, NESMOS Department, Faculty of Medicine and Psychology, "Sapienza" University of Rome, "Sant'Andrea" University Hospital, Rome, Italy.

Acute abdominal pain in children is the most common cause of emergency department admissions. Omental infarction is a rare cause of acute abdominal pain in this age group, accounting for approximately 15% of cases in children and 0.024-0.1% of cases of surgery for suspected appendicitis at the same age. Its clinical presentation may mimic similar diseases such as acute appendicitis, epiploic appendagitis, and mesenteric panniculitis. Ultrasound is the modality of choice for the initial evaluation of acute abdominal pain in pediatric patients and it can be used with confidence in the diagnosis and management of omental infarction in children. In this brief review, we focus on the main ultrasound findings and their diagnostic clue for omental infarction and its mimics.
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http://dx.doi.org/10.1007/s40477-020-00492-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588577PMC
December 2020
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