Francesca Micoli

Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).

Authors:

Francesca Mancini Gianmarco Gasperini Omar Rossi Maria Grazia Aruta Maria Michelina Raso Renzo Alfini Massimiliano Biagini Francesca Necchi Francesca Micoli

Sci Rep 2021 Jan 13;11(1):906. Epub 2021 Jan 13.

GSK Vaccines Institute for Global Health (GVGH), via Fiorentina 1, 53100, Siena, Italy.

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January 2021

Outer membrane vesicle vaccines.

Authors:

Francesca Micoli Calman A MacLennan

Semin Immunol 2020 08 9;50:101433. Epub 2020 Dec 9.

Bill & Melinda Gates Foundation, 62 Buckingham Gate, London, United Kingdom; Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.

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August 2020

Rational Design of a Glycoconjugate Vaccine against Group A .

Authors:

Roberta Di Benedetto Francesca Mancini Martina Carducci Gianmarco Gasperini Danilo Gomes Moriel Allan Saul Francesca Necchi Rino Rappuoli Francesca Micoli

Int J Mol Sci 2020 Nov 13;21(22). Epub 2020 Nov 13.

GSK Vaccines Institute for Global Health (GVGH), Via Fiorentina 1, 53100 Siena, Italy.

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November 2020

O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein.

Authors:

Francesca Micoli Carlo Giannelli Roberta Di Benedetto

Methods Mol Biol 2021 ;2183:267-304

GSK Vaccines Institute for Global Health, Siena, Italy.

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January 2021

GMMA Is a Versatile Platform to Design Effective Multivalent Combination Vaccines.

Authors:

Francesca Micoli Renzo Alfini Roberta Di Benedetto Francesca Necchi Fabiola Schiavo Francesca Mancini Martina Carducci Elena Palmieri Cristiana Balocchi Gianmarco Gasperini Brunella Brunelli Paolo Costantino Roberto Adamo Diego Piccioli Allan Saul

Vaccines (Basel) 2020 Sep 17;8(3). Epub 2020 Sep 17.

GSK Vaccines Institute for Global Health (GVGH) S.r.l., 53100 Siena, Italy.

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September 2020

Short Vi-polysaccharide abrogates T-independent immune response and hyporesponsiveness elicited by long Vi-CRM conjugate vaccine.

Authors:

Francesca Micoli Stefania P Bjarnarson Melissa Arcuri Audur Anna Aradottir Pind Gudbjorg J Magnusdottir Francesca Necchi Roberta Di Benedetto Martina Carducci Fabiola Schiavo Carlo Giannelli Ivan Pisoni Laura B Martin Giuseppe Del Giudice Calman A MacLennan Rino Rappuoli Ingileif Jonsdottir Allan Saul

Proc Natl Acad Sci U S A 2020 09 8;117(39):24443-24449. Epub 2020 Sep 8.

Technology Platform Unit, GSK Vaccines Institute for Global Health (GVGH), 53100 Siena, Italy.

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September 2020

Characterization of Competitive ELISA and Formulated Alhydrogel Competitive ELISA (FAcE) for Direct Quantification of Active Ingredients in GMMA-Based Vaccines.

Authors:

Omar Rossi Maria Grazia Aruta Alessandra Acquaviva Francesca Mancini Francesca Micoli Francesca Necchi

Methods Protoc 2020 Aug 31;3(3). Epub 2020 Aug 31.

GSK Vaccines Institute for Global Health s.r.l (GVGH), 53100 Siena, Italy.

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August 2020

OMV Vaccines and the Role of TLR Agonists in Immune Response.

Authors:

Francesca Mancini Omar Rossi Francesca Necchi Francesca Micoli

Int J Mol Sci 2020 Jun 21;21(12). Epub 2020 Jun 21.

GSK Vaccines Institute for Global Health s.r.l (GVGH), 53100 Siena, Italy.

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June 2020

Intra-Laboratory Evaluation of Luminescence Based High-Throughput Serum Bactericidal Assay (L-SBA) to Determine Bactericidal Activity of Human Sera against .

Authors:

Omar Rossi Eleonora Molesti Allan Saul Carlo Giannelli Francesca Micoli Francesca Necchi

High Throughput 2020 Jun 8;9(2). Epub 2020 Jun 8.

GSK Vaccines Institute for Global Health s.r.l (GVGH), Siena 53100, Italy.

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June 2020

Improving efficacy of glycoconjugate vaccines: from chemical conjugates to next generation constructs.

Authors:

Francesco Berti Francesca Micoli

Curr Opin Immunol 2020 Aug 30;65:42-49. Epub 2020 Apr 30.

GSK Vaccines Institute for Global Health S.r.l., Siena, Italy.

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August 2020

Development of a Specific and Sensitive HPAEC-PAD Method for Quantification of Vi Polysaccharide Applicable to other Polysaccharides Containing Amino Uronic Acids.

Authors:

Carlo Giannelli Maria Michelina Raso Elena Palmieri Antonia De Felice Federico Pippi Francesca Micoli

Anal Chem 2020 05 24;92(9):6304-6311. Epub 2020 Apr 24.

GSK Vaccines Institute for Global Health, Via Fiorentina 1, 53100 Siena, Italy.

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May 2020

Gold nanoparticles morphology does not affect the multivalent presentation and antibody recognition of Group A Streptococcus synthetic oligorhamnans.

Authors:

Olimpia Pitirollo Francesca Micoli Francesca Necchi Francesca Mancini Martina Carducci Roberto Adamo Claudio Evangelisti Laura Morelli Laura Polito Luigi Lay

Bioorg Chem 2020 06 3;99:103815. Epub 2020 Apr 3.

Department of Chemistry and CRC Materiali Polimerici (LaMPo), University of Milan, Via C. Golgi 19, 20133 Milan, Italy. Electronic address:

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June 2020

GMMA and Glycoconjugate Approaches Compared in Mice for the Development of a Vaccine against Serotype 6.

Authors:

Maria Michelina Raso Gianmarco Gasperini Renzo Alfini Fabiola Schiavo Maria Grazia Aruta Martina Carducci Maria Concetta Forgione Silvia Martini Paola Cescutti Francesca Necchi Francesca Micoli

Vaccines (Basel) 2020 Apr 3;8(2). Epub 2020 Apr 3.

GSK Vaccines Institute for Global Health (GVGH) S.r.l., via Fiorentina 1, 53100 Siena, Italy.

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April 2020

Structure, dynamics and immunogenicity of a catalytically inactive CC chemokine-degrading protease SpyCEP from .

Authors:

Sophie McKenna Enrico Malito Sarah L Rouse Francesca Abate Giuliano Bensi Emiliano Chiarot Francesca Micoli Francesca Mancini Danilo Gomes Moriel Guido Grandi Danuta Mossakowska Max Pearson Yingqi Xu James Pease Shiranee Sriskandan Immaculada Margarit Matthew J Bottomley Stephen Matthews

Comput Struct Biotechnol J 2020 13;18:650-660. Epub 2020 Mar 13.

Department of Life Sciences, Imperial College London, South Kensington Campus, SW7 2AZ, UK.

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March 2020

Glycoconjugate vaccines: current approaches towards faster vaccine design.

Authors:

Francesca Micoli Linda Del Bino Renzo Alfini Filippo Carboni Maria Rosaria Romano Roberto Adamo

Expert Rev Vaccines 2019 09 31;18(9):881-895. Epub 2019 Aug 31.

Research Centre, GSK , Siena , Italy.

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September 2019

Characterization of the Salmonella Typhimurium core oligosaccharide and its reducing end 3-deoxy-d-manno-oct-2-ulosonic acid used for conjugate vaccine production.

Authors:

Gianluigi De Benedetto Francesca Micoli Silvia Londero Laura Salvini Luisa Sturiale Domenico Garozzo Neil Ravenscroft Carlo Giannelli Paola Cescutti

Carbohydr Res 2019 Jul 6;481:43-51. Epub 2019 Jun 6.

Department of Life Sciences, University of Trieste, Via L. Giorgieri 1, Bdg C11, 34127, Trieste, Italy. Electronic address:

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July 2019

Comparative immunogenicity and efficacy of equivalent outer membrane vesicle and glycoconjugate vaccines against nontyphoidal .

Authors:

Francesca Micoli Simona Rondini Renzo Alfini Luisa Lanzilao Francesca Necchi Aurel Negrea Omar Rossi Cornelia Brandt Simon Clare Pietro Mastroeni Rino Rappuoli Allan Saul Calman A MacLennan

Proc Natl Acad Sci U S A 2018 10 27;115(41):10428-10433. Epub 2018 Sep 27.

Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7DQ, United Kingdom.

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October 2018

Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations.

Authors:

Emma Ispasanie Francesca Micoli Araceli Lamelas Dominique Keller Francesco Berti Riccardo De Riccio Roberta Di Benedettoi Simona Rondini Gerd Pluschke

Virulence 2018 ;9(1):1138-1149

a Swiss Tropical and Public Health Institute , Molecular Immunology Unit , Basel , Switzerland.

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October 2018

Multiple Techniques for Size Determination of Generalized Modules for Membrane Antigens from and .

Authors:

Gianluigi De Benedetto Paola Cescutti Carlo Giannelli Roberto Rizzo Francesca Micoli

ACS Omega 2017 Nov 21;2(11):8282-8289. Epub 2017 Nov 21.

GSK Vaccines Institute for Global Health (GVGH) S.r.l., Via Fiorentina 1, 53100 Siena, Italy.

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November 2017

Protein Carriers for Glycoconjugate Vaccines: History, Selection Criteria, Characterization and New Trends.

Authors:

Francesca Micoli Roberto Adamo Paolo Costantino

Molecules 2018 Jun 15;23(6). Epub 2018 Jun 15.

GSK, 53100 Siena, Italy.

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June 2018

Investigation on Sugar-Protein Connectivity in Salmonella O-Antigen Glycoconjugate Vaccines.

Authors:

Gianluigi De Benedetto Laura Salvini Stefano Gotta Paola Cescutti Francesca Micoli

Bioconjug Chem 2018 05 7;29(5):1736-1747. Epub 2018 May 7.

GSK Vaccines Institute for Global Health (GVGH) S.r.l. , via Fiorentina 1 , 53100 Siena , Italy.

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May 2018

Potential targets for next generation antimicrobial glycoconjugate vaccines.

Authors:

Francesca Micoli Paolo Costantino Roberto Adamo

FEMS Microbiol Rev 2018 05;42(3):388-423

GSK, Via Fiorentina 1, 53100 Siena.

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May 2018

IgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against Nontyphoidal Develop at Discordant Rates.

Authors:

Anna E Schager C Coral Dominguez-Medina Francesca Necchi Francesca Micoli Yun Shan Goh Margaret Goodall Adriana Flores-Langarica Saeeda Bobat Charlotte N L Cook Melissa Arcuri Arianna Marini Lloyd D W King Faye C Morris Graham Anderson Kai-Michael Toellner Ian R Henderson Constantino López-Macías Calman A MacLennan Adam F Cunningham

mBio 2018 03 6;9(2). Epub 2018 Mar 6.

Institute for Microbiology and Infection, School of Immunology and Immunotherapy, Institute for Biomedical Research, University of Birmingham, Birmingham, United Kingdom

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March 2018

Contribution of factor H-Binding protein sequence to the cross-reactivity of meningococcal native outer membrane vesicle vaccines with over-expressed fHbp variant group 1.

Authors:

Arianna Marini Omar Rossi Maria Grazia Aruta Francesca Micoli Simona Rondini Serafina Guadagnuolo Isabel Delany Ian R Henderson Adam F Cunningham Allan Saul Calman A MacLennan Oliver Koeberling

PLoS One 2017 25;12(7):e0181508. Epub 2017 Jul 25.

GSK Vaccines Institute for Global Health (GVGH), Siena, Italy.

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October 2017

Immunogenicity of a Bivalent Adjuvanted Glycoconjugate Vaccine against Typhimurium and Enteritidis.

Authors:

Fabio Fiorino Simona Rondini Francesca Micoli Luisa Lanzilao Renzo Alfini Francesca Mancini Calman A MacLennan Donata Medaglini

Front Immunol 2017 27;8:168. Epub 2017 Feb 27.

Laboratorio di Microbiologia Molecolare e Biotecnologia (LA.M.M.B.), Dipartimento di Biotecnologie Mediche, Università di Siena , Siena , Italy.

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February 2017

Bactericidal Immunity to Salmonella in Africans and Mechanisms Causing Its Failure in HIV Infection.

Authors:

Yun Shan Goh Francesca Necchi Colette M O'Shaughnessy Francesca Micoli Massimiliano Gavini Stephen P Young Chisomo L Msefula Esther N Gondwe Wilson L Mandala Melita A Gordon Allan J Saul Calman A MacLennan

PLoS Negl Trop Dis 2016 Apr 8;10(4):e0004604. Epub 2016 Apr 8.

School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

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April 2016

Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

Authors:

Omar Rossi Mariaelena Caboni Aurel Negrea Francesca Necchi Renzo Alfini Francesca Micoli Allan Saul Calman A MacLennan Simona Rondini Christiane Gerke

Clin Vaccine Immunol 2016 Apr 4;23(4):304-14. Epub 2016 Apr 4.

Novartis Vaccines Institute for Global Health, S.r.l., Siena, Italy

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April 2016

Differential Killing of Salmonella enterica Serovar Typhi by Antibodies Targeting Vi and Lipopolysaccharide O:9 Antigen.

Authors:

Peter J Hart Colette M O'Shaughnessy Matthew K Siggins Saeeda Bobat Robert A Kingsley David A Goulding John A Crump Hugh Reyburn Francesca Micoli Gordon Dougan Adam F Cunningham Calman A MacLennan

PLoS One 2016 7;11(1):e0145945. Epub 2016 Jan 7.

School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

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June 2016

Click Chemistry Applied to the Synthesis of Salmonella Typhimurium O-Antigen Glycoconjugate Vaccine on Solid Phase with Sugar Recycling.

Authors:

Giuseppe Stefanetti Allan Saul Calman A MacLennan Francesca Micoli

Bioconjug Chem 2015 Dec 18;26(12):2507-13. Epub 2015 Nov 18.

GSK Vaccines Institute for Global Health (former Novartis Vaccines Institute for Global Health NVGH) , Via Fiorentina 1, 53100 Siena, Italy.

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December 2015

Strain Selection for Generation of O-Antigen-Based Glycoconjugate Vaccines against Invasive Nontyphoidal Salmonella Disease.

Authors:

Luisa Lanzilao Giuseppe Stefanetti Allan Saul Calman A MacLennan Francesca Micoli Simona Rondini

PLoS One 2015 7;10(10):e0139847. Epub 2015 Oct 7.

Sclavo Behring Vaccines Institute for Global Health S.r.l., a GSK company (formerly Novartis Vaccines Institute for Global Health S.r.l), Via Fiorentina 1, 53100, Siena, Italy.

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June 2016

Sugar-Protein Connectivity Impacts on the Immunogenicity of Site-Selective Salmonella O-Antigen Glycoconjugate Vaccines.

Authors:

Giuseppe Stefanetti Qi-Ying Hu Aimee Usera Zack Robinson Martin Allan Alok Singh Hidetomo Imase Jennifer Cobb Huili Zhai Douglas Quinn Ming Lei Allan Saul Roberto Adamo Calman A MacLennan Francesca Micoli

Angew Chem Int Ed Engl 2015 Nov 9;54(45):13198-203. Epub 2015 Sep 9.

Sclavo Behring Vaccines Institute For Global Health S.r.l., A GSK Company, Via Fiorentina 1, 53100 Siena (Italy).

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November 2015

Monoclonal Antibodies of a Diverse Isotype Induced by an O-Antigen Glycoconjugate Vaccine Mediate In Vitro and In Vivo Killing of African Invasive Nontyphoidal Salmonella.

Authors:

Yun Shan Goh Simon Clare Francesca Micoli Allan Saul Pietro Mastroeni Calman A MacLennan

Infect Immun 2015 Sep 13;83(9):3722-31. Epub 2015 Jul 13.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom

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September 2015

Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.

Authors:

Robert S Onsare Francesca Micoli Luisa Lanzilao Renzo Alfini Chinyere K Okoro Anne W Muigai Gunturu Revathi Allan Saul Samuel Kariuki Calman A MacLennan Simona Rondini

PLoS Negl Trop Dis 2015 Mar 4;9(3):e0003573. Epub 2015 Mar 4.

Novartis Vaccines Institute for Global Health (NVGH), Siena, Italy.

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March 2015

Simplified low-cost production of O-antigen from Salmonella Typhimurium Generalized Modules for Membrane Antigens (GMMA).

Authors:

Eleonora Meloni Anna Maria Colucci Francesca Micoli Luigi Sollai Massimiliano Gavini Allan Saul Vito Di Cioccio Calman A MacLennan

J Biotechnol 2015 Mar 3;198:46-52. Epub 2015 Feb 3.

Novartis Vaccines Institute for Global Health, Via Fiorentina 1, 53100 Siena, Italy. Electronic address:

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March 2015

Vaccines against invasive Salmonella disease: current status and future directions.

Authors:

Calman A MacLennan Laura B Martin Francesca Micoli

Hum Vaccin Immunother 2014 7;10(6):1478-93. Epub 2014 May 7.

Novartis Vaccines Institute for Global Health; Siena, Italy.

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June 2015

Structural analysis of O-polysaccharide chains extracted from different Salmonella Typhimurium strains.

Authors:

Francesca Micoli Neil Ravenscroft Paola Cescutti Giuseppe Stefanetti Silvia Londero Simona Rondini Calman A Maclennan

Carbohydr Res 2014 Feb 11;385:1-8. Epub 2013 Dec 11.

Novartis Vaccines Institute for Global Health, Via Fiorentina 1, I-53100 Siena, Italy; Medical Research Council Centre for Immune Regulation, Institute of Biomedical Research, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.

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February 2014