Florian M Wurm

Florian M Wurm

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Florian M Wurm

Publications by authors named "Florian M Wurm"

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Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.

Mol Ther Methods Clin Dev 2019 Jun 22;13:14-26. Epub 2018 Nov 22.

Department of Clinical Neurosciences, Laboratory of Neurotherapies and Neuromodulation (LNTM), Lausanne University Hospital (CHUV), 1011 Lausanne, Switzerland.

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https://linkinghub.elsevier.com/retrieve/pii/S23290501183011
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http://dx.doi.org/10.1016/j.omtm.2018.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305802PMC
June 2019

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.

Biotechnol Lett 2018 Jan 9;40(1):85-92. Epub 2017 Oct 9.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-017-2440-0DOI Listing
January 2018

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes.

Biotechnol Prog 2017 01 31;33(1):192-200. Epub 2016 Oct 31.

Laboratory of Cellular Biotechnology (LBTC), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

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http://dx.doi.org/10.1002/btpr.2375DOI Listing
January 2017

Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.

Biotechnol Prog 2015 Nov-Dec;31(6):1571-8. Epub 2015 Aug 25.

Laboratory for Cellular Biotechnology (LBTC) and Protein Expression Core Facility (PECF), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.

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http://dx.doi.org/10.1002/btpr.2152DOI Listing
October 2016

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system.

Biotechnol Prog 2016 09 13;32(5):1308-1317. Epub 2016 Jul 13.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

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http://dx.doi.org/10.1002/btpr.2319DOI Listing
September 2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation.

Biotechnol Prog 2016 09 11;32(5):1174-1180. Epub 2016 Aug 11.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, CH-1015, Switzerland.

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http://dx.doi.org/10.1002/btpr.2328DOI Listing
September 2016

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension.

Anal Biochem 2016 07 27;505:26-8. Epub 2016 Apr 27.

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.ab.2016.04.009DOI Listing
July 2016

Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.

Biotechnol Bioeng 2016 06 9;113(6):1234-43. Epub 2015 Dec 9.

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.25888DOI Listing
June 2016

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.

Biotechnol Prog 2015 Mar-Apr;31(2):541-9. Epub 2015 Feb 26.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/btpr.2064DOI Listing
January 2016

Production of active glycosylation-deficient γ-secretase complex for crystallization studies.

Biotechnol Bioeng 2015 Dec 14;112(12):2516-26. Epub 2015 Jul 14.

Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.25675DOI Listing
December 2015

A simple plasmid-based transient gene expression method using High Five cells.

J Biotechnol 2015 Dec 23;216:67-75. Epub 2015 Oct 23.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.jbiotec.2015.10.007DOI Listing
December 2015

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells.

BMC Proc 2011 22;5 Suppl 8:P35. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P35DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284955PMC
April 2015

kLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors.

BMC Proc 2011 22;5 Suppl 8:P36. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P36DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397689PMC
April 2015

Transient transfection of insect Sf-9 cells in TubeSpin(®) bioreactor 50 tubes.

BMC Proc 2011 22;5 Suppl 8:P37. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284885PMC
April 2015

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin(®) bioreactors.

BMC Proc 2011 22;5 Suppl 8:P38. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284980PMC
April 2015

Hydrodynamic stress in orbitally shaken bioreactors.

BMC Proc 2011 22;5 Suppl 8:P39. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284983PMC
April 2015

CHO cell lines generated by PiggyBac transposition.

BMC Proc 2011 22;5 Suppl 8:P31. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland ; ExcellGene SA, CH-1870 Monthey, Switzerland.

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http://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-
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http://dx.doi.org/10.1186/1753-6561-5-S8-P31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284968PMC
April 2015

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells.

BMC Proc 2011 22;5 Suppl 8:P32. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology (LBTC), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284971PMC
April 2015

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells.

BMC Proc 2011 22;5 Suppl 8:P33. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédéral de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285026PMC
April 2015

Rapid recombinant protein production from pools of transposon-generated CHO cells.

BMC Proc 2011 22;5 Suppl 8:P34. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland ; ExcellGene SA, CH-1870 Monthey, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P34DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285008PMC
April 2015

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools.

J Biotechnol 2015 Apr 7;200:61-9. Epub 2015 Mar 7.

Laboratory for Cellular Biotechnology, CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.jbiotec.2015.03.001DOI Listing
April 2015

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells.

Int J Mol Cell Med 2014 ;3(3):146-56

Laboratory of Cellular Biotechnology, Ècole Polytechnique Fédérale de Lausanne, Switzerland.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170488PMC
October 2014

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects.

Drug Discov Today Technol 2008 ;5(2-3):e37-42

Institute of Bioengineering, Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic

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http://dx.doi.org/10.1016/j.ddtec.2008.12.003DOI Listing
July 2014

Virus-free transient protein production in Sf9 cells.

J Biotechnol 2014 Feb 11;171:61-70. Epub 2013 Dec 11.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.jbiotec.2013.11.018DOI Listing
February 2014

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Protein Expr Purif 2013 Nov 8;92(1):67-76. Epub 2013 Sep 8.

Protein Expression Core Facility, EPFL, CH-1015 Lausanne, Switzerland; Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.pep.2013.09.001DOI Listing
November 2013

DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine.

J Control Release 2013 Aug 1;169(3):276-88. Epub 2013 Feb 1.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.jconrel.2013.01.019DOI Listing
August 2013

Highly efficient production of the Alzheimer's γ-secretase integral membrane protease complex by a multi-gene stable integration approach.

Biotechnol Bioeng 2013 Jul 15;110(7):1995-2005. Epub 2013 Feb 15.

Laboratory of Molecular & Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.24851DOI Listing
July 2013

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity.

N Biotechnol 2013 Jan 6;30(2):238-45. Epub 2012 Jun 6.

Merck-Serono S.A., Biotech Process Sciences Group, CH-1804 Corsier-sur-Vevey, Switzerland.

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http://dx.doi.org/10.1016/j.nbt.2012.05.021DOI Listing
January 2013

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells.

Anal Biochem 2012 Nov 6;430(2):138-40. Epub 2012 Sep 6.

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.ab.2012.08.028DOI Listing
November 2012

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.

Biotechnol Bioeng 2012 Sep 30;109(9):2271-8. Epub 2012 Mar 30.

Laboratory for Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.24494DOI Listing
September 2012

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells.

N Biotechnol 2012 May 6;29(4):471-6. Epub 2012 Mar 6.

École Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory of Cellular Biotechnology, Station 6, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.nbt.2012.02.003DOI Listing
May 2012

Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups.

Macromol Biosci 2012 May 13;12(5):628-36. Epub 2012 Mar 13.

Laboratoire des Polymères, Institut des Matériaux et Institut des Sciences et Ingénieries Chimiques, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/mabi.201100404DOI Listing
May 2012

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells.

Biotechnol Lett 2012 Apr 30;34(4):619-26. Epub 2011 Nov 30.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), 1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-011-0809-zDOI Listing
April 2012

Large-scale transfection of mammalian cells.

Methods Mol Biol 2012 ;801:13-26

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/978-1-61779-352-3_2DOI Listing
February 2012

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors.

N Biotechnol 2012 Feb 26;29(3):387-94. Epub 2011 Nov 26.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.nbt.2011.10.010DOI Listing
February 2012

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry.

Anal Chem 2011 Dec 4;83(23):8919-27. Epub 2011 Nov 4.

Biomolecular Mass Spectrometry Laboratory, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1021/ac201293mDOI Listing
December 2011

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.

Biotechnol Bioeng 2011 Sep 25;108(9):2141-50. Epub 2011 Apr 25.

École Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH J2-506, Station 6, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.23167DOI Listing
September 2011

First CHO genome.

Nat Biotechnol 2011 Aug 5;29(8):718-20. Epub 2011 Aug 5.

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http://dx.doi.org/10.1038/nbt.1943DOI Listing
August 2011

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

N Biotechnol 2011 Jul 19;28(4):382-90. Epub 2011 Feb 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.nbt.2011.02.004DOI Listing
July 2011

Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors.

Eur J Pharm Biopharm 2011 Jun 20;78(2):184-8. Epub 2011 Jan 20.

ExcellGene SA, Monthey, Switzerland.

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http://dx.doi.org/10.1016/j.ejpb.2011.01.005DOI Listing
June 2011

A collagen-poly(lactic acid-co-ɛ-caprolactone) hybrid scaffold for bladder tissue regeneration.

Biomaterials 2011 Jun 4;32(16):3969-76. Epub 2011 Mar 4.

Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory for Experimental Pediatric Urology and Regenerative Medicine & Pharmacobiology, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.biomaterials.2011.02.012DOI Listing
June 2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension.

Biotechnol Lett 2011 May 26;33(5):897-902. Epub 2011 Jan 26.

Laboratory of Cellular Biotechnology, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, CH J2 506, Station 6, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-011-0527-6DOI Listing
May 2011

A simple high-yielding process for transient gene expression in CHO cells.

J Biotechnol 2011 Apr 15;153(1-2):22-6. Epub 2011 Mar 15.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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http://ac.els-cdn.com/S016816561100112X/1-s2.0-S016816561100
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http://linkinghub.elsevier.com/retrieve/pii/S016816561100112
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http://dx.doi.org/10.1016/j.jbiotec.2011.03.001DOI Listing
April 2011

Generation of stable, high-producing CHO cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture.

Biotechnol Bioeng 2011 Mar 23;108(3):600-10. Epub 2010 Nov 23.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

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http://doi.wiley.com/10.1002/bit.22968
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http://dx.doi.org/10.1002/bit.22968DOI Listing
March 2011

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells.

Biotechnol Lett 2010 Nov 24;32(11):1587-92. Epub 2010 Jun 24.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH J2-506, Station 6, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-010-0331-8DOI Listing
November 2010

25 years of recombinant proteins from reactor-grown cells - where do we go from here?

Biotechnol Adv 2009 Nov-Dec;27(6):1023-1027. Epub 2009 May 20.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland; ExcellGene SA, CH-1870 Monthey, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.biotechadv.2009.05.008DOI Listing
January 2010

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells.

Vaccine 2009 Oct 24;27(46):6415-9. Epub 2009 Jun 24.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.vaccine.2009.06.019DOI Listing
October 2009

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells.

N Biotechnol 2008 Oct-Dec;25(2-3):162-6. Epub 2008 Aug 27.

Ecole Polytechnique Fédérale de Lausanne, Laboratory of Cellular Biotechnology, Institute of Bioengineering, Faculty of Life Sciences, Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.nbt.2008.08.007DOI Listing
February 2009

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

Biotechnol Bioeng 2008 Dec;101(5):937-45

Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, Laboratory of Cellular Biotechnology, CHJ2-506, Station 6, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.21972DOI Listing
December 2008

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells.

Mol Biotechnol 2008 Oct 10;40(2):136-43. Epub 2008 Jun 10.

Laboratory of Cellular Biotechnology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s12033-008-9069-0DOI Listing
October 2008

Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures.

Biotechnol Bioeng 2008 Sep;101(1):182-9

Laboratory of Cellular Biotechnology, Institute of Bioengineering, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.21882DOI Listing
September 2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions.

Nucleic Acids Res 2008 Sep 10;36(15):e96. Epub 2008 Jul 10.

Ecole Polytechnique Fédérale de Lausanne, Laboratory of Cellular Biotechnology, Institute of Bioengineering, Faculty of Life Sciences, Lausanne, Switzerland.

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http://dx.doi.org/10.1093/nar/gkn423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528171PMC
September 2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells.

Biotechnol Prog 2008 Mar-Apr;24(2):458-65. Epub 2008 Jan 26.

Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

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http://dx.doi.org/10.1021/bp070286cDOI Listing
June 2008

Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000 L by surface aeration with air.

N Biotechnol 2008 Jun 19;25(1):68-75. Epub 2008 May 19.

Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Laboratory of Cellular Biotechnology, CH J2-506, Station 6, CH-1015 Lausanne, Switzerland.

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https://linkinghub.elsevier.com/retrieve/pii/S18716784080000
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http://dx.doi.org/10.1016/j.nbt.2008.03.001DOI Listing
June 2008

Novel orbital shake bioreactors for transient production of CHO derived IgGs.

Biotechnol Prog 2007 Nov-Dec;23(6):1340-6. Epub 2007 Oct 3.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://www.tpp.ch/page/downloads/TubeSpin/Stettler_Novel-Orb
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http://pubs.acs.org/cgi-bin/doilookup/?10.1021/bp070219i
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http://dx.doi.org/10.1021/bp070219iDOI Listing
March 2008

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

Biotechnol Bioeng 2008 Feb;99(3):721-7

Ecole Polytechnique Fédérale de Lausanne, Laboratory of Cellular Biotechnology, Institute of Bioengineering, Faculty of Life Sciences, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.21596DOI Listing
February 2008

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells.

Biotechnol Lett 2007 Nov 17;29(11):1713-21. Epub 2007 Jul 17.

ExcellGene S.A, Route de l'ile-au-bois 1A, Monthey, Valais, 1870, Switzerland.

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http://dx.doi.org/10.1007/s10529-007-9441-3DOI Listing
November 2007

Manufacturing of biopharmaceuticals and implications for biosimilars.

Authors:
Florian M Wurm

Kidney Blood Press Res 2007 27;30 Suppl 1:6-8. Epub 2007 Aug 27.

Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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http://dx.doi.org/10.1159/000107094DOI Listing
November 2007

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives.

Biotechnol Lett 2007 May 19;29(5):677-84. Epub 2007 Jan 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland.

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May 2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems.

Biotechnol Lett 2007 May 20;29(5):703-11. Epub 2007 Feb 20.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Laboratory of Cellular Biotechnology, 1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-006-9298-xDOI Listing
May 2007

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells.

Biotechnol Bioeng 2006 Dec;95(6):1228-33

Ecole Polytechnique Fédérale de Lausanne, Faculty of Life Sciences, Institute of Bioengineering, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.21071DOI Listing
December 2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.

Biotechnol Lett 2006 Nov 29;28(22):1827-33. Epub 2006 Sep 29.

Institute of Biochemistry, Chemin des Boveresses 155, 1066, Epalinges, Switzerland.

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http://link.springer.com/10.1007/s10529-006-9167-7
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November 2006

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.

J Control Release 2006 Nov 22;116(1):96-104. Epub 2006 Sep 22.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.jconrel.2006.09.006DOI Listing
November 2006

Polyethylenimine-based quality control assay for plasmid DNA.

Anal Biochem 2006 Sep 19;356(2):309-11. Epub 2006 May 19.

Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.ab.2006.05.002DOI Listing
September 2006

Generation of recombinant Chinese hamster ovary cell lines by microinjection.

Biotechnol Lett 2006 Mar;28(6):373-82

Laboratory of Cellular Biotechnology, Institute of Biological Engineering and Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland.

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http://dx.doi.org/10.1007/s10529-005-6062-6DOI Listing
March 2006

Large-scale transient expression of therapeutic proteins in mammalian cells.

Methods Mol Biol 2005 ;308:87-98

Novartis Pharma Research CT/BMP, Basel, Switzerland.

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http://dx.doi.org/10.1385/1-59259-922-2:087DOI Listing
September 2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production.

Biotechnol Prog 2005 Jan-Feb;21(1):148-53

Laboratory of Cellular Biotechnology, Institute for Biological Engineering and Biotechhnology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1021/bp049830xDOI Listing
July 2005

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells.

J Biotechnol 2005 Apr;117(1):21-9

Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.jbiotec.2005.01.006DOI Listing
April 2005

Orbital shaker technology for the cultivation of mammalian cells in suspension.

Biotechnol Bioeng 2005 Feb;89(4):400-6

Swiss Federal Institute of Technology, Institute of Biological Engineering and Biotechnology, Laboratory of Cellular Biotechnology, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.20358DOI Listing
February 2005

Low-temperature pausing of cultivated mammalian cells.

Biotechnol Bioeng 2005 Jan;89(2):157-63

Swiss Federal Institute of Technology Lausanne, Faculty of Basic Sciences, Institute of Biological and Chemical Process Sciences, CH1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.20320DOI Listing
January 2005

A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles.

Anal Biochem 2004 Nov;334(1):196-8

Swiss Federal Institute of Technology Lausanne, Laboratory of Cellular Biotechnology, Lausanne VD1015, Switzerland.

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http://dx.doi.org/10.1016/j.ab.2004.07.020DOI Listing
November 2004

Production of recombinant protein therapeutics in cultivated mammalian cells.

Authors:
Florian M Wurm

Nat Biotechnol 2004 Nov;22(11):1393-8

Laboratory of Cellular Biotechnology, Institute of Biological Engineering and Biotechnology, Faculty of Life Sciences, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland. florian@

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http://dx.doi.org/10.1038/nbt1026DOI Listing
November 2004

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells.

Gene 2004 Oct;341:227-34

Laboratory of Cellular Biotechnology, Swiss Federal Institute of Technology Lausanne (EPFL), CH1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.gene.2004.06.054DOI Listing
October 2004

Serum-free large-scale transient transfection of CHO cells.

Biotechnol Bioeng 2004 Aug;87(4):537-45

Laboratory of Cellular Biotechnology, IGBB, Faculty of Life Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland.

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http://doi.wiley.com/10.1002/bit.20161
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August 2004

Human therapeutic proteins from silkworms.

Authors:
Florian M Wurm

Nat Biotechnol 2003 Jan;21(1):34-5

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http://dx.doi.org/10.1038/nbt0103-34DOI Listing
January 2003

Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example.

Biotechnol Bioeng 2002 Mar;77(5):528-37

Laboratory of Cellular Biotechnology, Department of Chemical Engineering, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.10108DOI Listing
March 2002

100-liter transient transfection.

Cytotechnology 2002 Jan;38(1-3):15-21

LBTC, Center of Biotechnology, EPFL, Lausanne, Switzerland.

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http://dx.doi.org/10.1023/A:1021173124640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449920PMC
January 2002

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi.

Cytotechnology 2002 Jan;38(1-3):57-62

Laboratoire de Biotechnologie Cellulaire, Institute of Biological and Chemical Processes, Faculty of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland,

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http://link.springer.com/10.1023/A:1021197830091
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449926PMC
January 2002