Publications by authors named "Fernanda Maria Policarpo Tonelli"

1 Publications

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Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis.

Sci Rep 2017 03 20;7:44317. Epub 2017 Mar 20.

Cell Signaling &Nanobiotechnology Laboratory, Department of Biochemistry &Immunology, Federal University of Minas Gerais, Belo Horizonte, Brazil.

Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transduction of gametes (GT), and fertilised egg transduction (ET). The SC protocol using larvae generates animals with sustained production of modified sperm (80% of animals with 77% maximum sperm fluorescence [MSF]), but is a time-consuming protocol (sexual maturity in Nile tilapia is achieved at 6 months of age). GT is a faster technique, but the modified gamete production is temporary (70% of animals with 52% MSF). ET is an easier way to obtain mosaic transgenic animals compared to microinjection of eggs, but non-site-directed integration in the fish genome can be a problem. In this study, PI3Kc2α gene disruption impaired development during the embryo stage and caused premature death. The manipulator should choose a technique based on the time available for transgenic obtainment and if this generation is required to be continuous or not.
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http://dx.doi.org/10.1038/srep44317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357942PMC
March 2017
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