Publications by authors named "Fengying Ye"

8 Publications

  • Page 1 of 1

A high-contrast photoacoustic agent with near-infrared emission.

Methods Enzymol 2021 19;657:223-247. Epub 2021 Jul 19.

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, PR China. Electronic address:

Benzobisthiadiazole as a typical electron acceptor, has been widely used to design fluorescent dyes and photoacoustic (PA) agents. With the strategy of constructing donor-acceptor-donor (D-A-D) type of electron characteristics, benzobisthiadiazole derivatives tend to behave stable in near-infrared absorption and emission, which is beneficial to PA imaging. In this chapter, two molecular design strategies are combined to improve the photoacoustic imaging effects of new PA contrast agent IR-1302 NPs, by installing strengthened conjugated bridges and electron donors. The nanoparticles exhibit high-contrast noninvasive photoacoustic imaging in tumor models with longer wavelength absorption and emission and show potential as a clinic contrast agent.
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http://dx.doi.org/10.1016/bs.mie.2021.06.037DOI Listing
August 2021

Microcystic pattern and shadowing are independent predictors of ovarian borderline tumors and cystadenofibromas in ultrasound.

Eur Radiol 2021 Jan 11;31(1):45-54. Epub 2020 Aug 11.

Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Licheng District, Quanzhou, 362000, Fujian, China.

Objectives: To determine the sonographic characteristics of borderline tumors (BoTs) and cystadenofibromas (CAFs).

Methods: Preoperative sonograms from consecutive patients who had at least one primary epithelial tumor in the adnexa were retrospectively collected. All tumors were described using the International Ovarian Tumor Analysis terminology. Ultrasound variables were tested using multinomial logistic regression after univariate analysis.

Results: A total of 650 patients were included in this study. Of these, 110 had a CAF, 128 had a BoT, 249 had a cystadenoma (CAD), and 163 had a cystadenocarcinoma (CAC). Nearly half of CAFs and more than half of BoTs and CACs appeared to be unilocular and multilocular solid on the ultrasound images, while CADs were predominantly uni- or multilocular (p < 0.001). Overall, shadowing was identified in 82/650 cases. Sixty-five of 110 (59.1%) CAFs exhibited an acoustic shadow, compared with only 4/249 (1.6%) in CADs, 7/128 (5.5%) in BoTs, and 6/163 (3.7%) in CACs (p < 0.001). Furthermore, 112/650 cases demonstrated microcystic pattern (MCP). Sixty-eight of 128 (53.1%) BoTs exhibited MCP, compared with only 5/249 (2.0%) in CADs, 19/163 (11.7%) in CACs, and 20/110 (18.2%) in CAFs (p < 0.001). Logistic regression analysis revealed that shadowing is an independent predictor of CAFs, while MCP is an independent predictor of BoTs.

Conclusions: Sonographic findings for CAFs and BoTs were complex and partly overlapped with those for CACs. However, proper recognition and utilization of shadowing or MCP may help to correctly discriminate CAFs and BoTs.

Key Points: • Sonographic findings for borderline tumors and cystadenofibromas are complex and mimic malignancy. • Microcystic pattern and shadowing are independent predictors of borderline tumors and cystadenofibromas respectively.
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http://dx.doi.org/10.1007/s00330-020-07113-zDOI Listing
January 2021

Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Oct 12;239:118465. Epub 2020 May 12.

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China. Electronic address:

A commercial cyanine dye IR-780 and a thioether-containing dicarboxylic acid ligand were used to construct the near-infrared fluorescent probe, which was used as a near-infrared fluorescent indicator for the determination of mercury ions in water and in living cells. This indicator displayed high specificity towards Hg without any interference from other detecting species. Especially, the emission at 790 nm dramatically increased more than 25 times after interacting with Hg. The binding experiment showed that the indicator formed 1:1 complex with Hg. More, this indicator could be applied in the visualization of Hg in living cells and measuring the Hg concentration of tap-water sample.
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http://dx.doi.org/10.1016/j.saa.2020.118465DOI Listing
October 2020

Determination of lipid mediators in breast cancer cells using lyophilization-supercritical fluid extraction online coupled with supercritical fluid chromatography-quadrupole tandem mass spectrometry.

J Sep Sci 2020 May 21;43(9-10):1800-1807. Epub 2019 Nov 21.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, P. R. China.

A lyophilization-supercritical fluid extraction coupled with supercritical fluid chromatography-quadrupole tandem mass spectrometry online method was developed for the determination of lipid mediators in breast cancer cells. Supercritical fluid extraction was applied to the cell samples for the first time due to the use of lyophilization. The conditions of supercritical fluid extraction and supercritical fluid chromatography-quadrupole tandem mass spectrometry were investigated systematically. Under the optimized conditions, all the calibration curves for the lipid mediators showed good linearity (correlation coefficient > 0.99). The limits of detection and the limits of quantification were in the range of 0.190-5.36 pg and 0.560-16.2 pg, respectively. The recoveries were in the range of 70.3-125%. The relative standard deviations of the precision ranged from 1.49-18.7% and the accuracies were higher than 84%. Compared with liquid-liquid extraction coupled with liquid chromatography and tandem mass spectrometry method, the present approach reduced the manual labor and obtained higher sensitivity as well as higher extraction recoveries for all 15 lipid mediators. Finally, the online method was applied to the quantification of lipid mediators in breast cancer cells and normal mammary epithelial cells. On the basis of the results, this lyophilization-supercritical fluid extraction online coupled with supercritical fluid chromatography-quadrupole tandem mass spectrometry method showed great promise in the analysis of lipid mediators in complex biological samples.
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http://dx.doi.org/10.1002/jssc.201900934DOI Listing
May 2020

Tetraphenylene-Coated Near-Infrared Benzoselenodiazole Dye: AIE Behavior, Mechanochromism, and Bioimaging.

Org Lett 2019 09 20;21(18):7213-7217. Epub 2019 Aug 20.

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education; Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis; International Joint Research Center for Intelligent Biosensing Technology and Health; College of Chemistry , Central China Normal University , Wuhan 430079 , P.R. China.

A D-π-A-π-D type of tetraphenylene-coating benzoselenodiazole fluorescence dye with near-infrared emission has been designed and constructed. This dye shows an obvious aggregation-induced-emission behavior. In the solid state, it exhibits a reversible mechanochromism with the changes of near-infrared emission. Furthermore, this dye can be used to track the lysosomes of living cells and images in vivo.
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http://dx.doi.org/10.1021/acs.orglett.9b02292DOI Listing
September 2019

Identification of the major metabolites of (R)-salbutamol in human urine, plasma and feces using ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

J Sep Sci 2019 Aug 7. Epub 2019 Aug 7.

Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, P. R. China.

(R)-Salbutamol is a selective β2-adrenoreceptor agonist, which produces a short-acting bronchodilator effect and is widely used for the treatment of respiratory diseases in humans. Drug metabolism and identification of the metabolites play an essential role in the evaluation of the overall efficacy and safety of the drugs in clinical practices. There are few reports on the identification of major metabolites of (R)-salbutamol in humans, and the number of identified metabolites is very limited. In this research, a method of ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed for the discovery and identification of (R)-salbutamol and its major metabolites in human biological samples. Totally, twelve metabolites of (R)-salbutamol were found and identified and all the metabolites could be found in urine, one metabolite in plasma and two metabolites in feces. Among all the metabolites, eight metabolites have never been reported before. The results indicated that (R)-salbutamol was mainly metabolized through isomerization, oxidation, reduction, glucuronidation, and sulfation pathways in vivo. The possible metabolic pathways of (R)-salbutamol were subsequently presented in this study, which contribute to a better understanding of the metabolism of (R)-salbutamol in humans.
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http://dx.doi.org/10.1002/jssc.201900330DOI Listing
August 2019

Advances of supercritical fluid chromatography in lipid profiling.

J Pharm Anal 2019 Feb 17;9(1):1-8. Epub 2018 Nov 17.

School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

Supercritical fluid chromatography (SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development.
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http://dx.doi.org/10.1016/j.jpha.2018.11.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355828PMC
February 2019

Regulating glutathione-responsiveness of naphthalimide-based fluorescent probes by an oxidation strategy.

Org Biomol Chem 2018 08;16(30):5517-5523

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.

Two naphthalimide-based fluorescent probes containing a thiomorpholine (Np-NS) or a sulfoxide-morpholine (Np-NSO) component are reported. The morpholine unit of non-fluorescent Np-NS and Np-NSO can transform into sulphone-morpholine and be accompanied by blue fluorescence upon oxidative stress, ascribed to the formation of sulphone-morpholine on probes. This sensing behavior displays that they can selectively respond to glutathione to generate a green emission by a sulfonamide-based detection moiety both in vitro and in living cells. Interestingly, the different oxidation states of a sulphur atom on a thiomorpholine ring can be utilized to regulate responsiveness of these probes towards glutathione. Such an oxidation strategy would provide a possibility for enhancing the response rate.
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http://dx.doi.org/10.1039/c8ob01258jDOI Listing
August 2018
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