Publications by authors named "Feng Zhang"

4,317 Publications

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Comparative transcriptome analysis reveals the resistance regulation mechanism and fungicidal activity of the fungicide phenamacril in Fusarium oxysporum.

Sci Rep 2022 Jun 30;12(1):11081. Epub 2022 Jun 30.

Key Laboratory of Pesticide, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Fusarium oxysporum (Fo) is an important species complex of soil-borne pathogenic fungi that cause vascular wilt diseases of agricultural crops and some opportunistic diseases of humans. The fungicide phenamacril has been extensively reported to have antifungal activity against Fusarium graminearum and Fusarium fujikuroi. In this study, we found that the amino acid substitutions (V151A and S418T) in Type I myosin FoMyo5 cause natural low resistance to phenamacril in the plant pathogenic Fo isolates. Therefore, we compared the transcriptomes of two phenamacril-resistant Fo isolates FoII5, Fo1st and one phenamacril-sensitive isolate Fo3_a after 1 μg/mL phenamacril treatment. Among the 2728 differentially expressed genes (DEGs), 14 DEGs involved in oxidation-reduction processes and MFS transporters, were significantly up-regulated in phenamacril-resistant isolates. On the other hand, 14 DEGs involved in ATP-dependent RNA helicase and ribosomal biogenesis related proteins, showed significantly down-regulated expression in both phenamacril-resistant and -sensitive isolates. These results indicated that phenamacril not only seriously affected the cytoskeletal protein binding and ATPase activity of sensitive isolate, but also suppressed ribosome biogenesis in all the isolates. Hence, this study helps us better understand resistance regulation mechanism and fungicidal activity of phenamacril and provide reference for the development of new fungicides to control Fo.
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http://dx.doi.org/10.1038/s41598-022-15188-5DOI Listing
June 2022

Toxicity of chemical pesticides commonly used in maize to Trichogramma ostriniae (Hymenoptera: Trichogrammatidae), an egg parasitoid of Asian corn borer.

Ecotoxicol Environ Saf 2022 Jun 27;241:113802. Epub 2022 Jun 27.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; MARA-CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

Chemical pesticides are largely applied worldwide for the control of agricultural pests, and often cause lethal and sublethal side effects on natural enemies. To determine the compatibility of chemical pesticides, commonly used by maize growers, with Trichogramma ostriniae, the toxicities of four herbicides (pendimethalin, topramezone, glyphosate, and atrazine), three insecticides (imidacloprid, emamectin benzoate, and ethiofencarb), and three fungicides (propiconazole, benzoyl cyclazole, and difenoconazole) were tested under laboratory conditions, measuring residual toxicity to T. ostriniae adults, their lethal effects on the parasitoid's eggs inside the host egg, and on the reproduction of the parasitoid's female offspring. Three tested pesticides including ethiofencarb, glyphosate, and benzoyl cyclazole were slightly harmful to T. ostriniae adults, whereas the other seven pesticides were moderately harmful. The pesticide residues of ethiofencarb, and emamectin benzoate, showed slightly persistence of toxicity to T. ostriniae adults, in contrast to the remaining pesticides. Glyphosate, emamectin benzoate, imidacloprid, ethiofencarb, and propiconazole were classified as harmless, causing < 30% reduction in emergence rate of wasps from host eggs, while atrazine, pendimethalin, topramezone, benzoyl cyclazole, and difenoconazole were classified as slightly harmful, causing 31-52% reduction in parasitoid emergence rates. Furthermore, the number of eggs subsequently laid by T. ostriniae females that had emerged from parasitized host eggs treated with atrazine was significantly reduced compared to which of untreated parasitized host eggs. No negative effects were observed with atrazine treatment on the emergence rates of F wasps, their wing deformity rates and sex ratios. The data presented here indicate that the safety interval between pesticide applications and releases of T. ostriniae wasps is 2-3 weeks, which provide valuable information in designing a sound maize integrated pest management program that combines chemical pesticide applications with augmentative releases of T. ostriniae wasps.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113802DOI Listing
June 2022

Positive affect decreases the negative association between social mobility belief and physical health among Chinese rural-to-urban migrant adolescents.

Psychoneuroendocrinology 2022 Jun 22;143:105846. Epub 2022 Jun 22.

Institute of Developmental Psychology, Faculty of Psychology, Beijing Normal University, Beijing, China; Beijing Key Laboratory of Applied Experimental Psychology, National Demonstration Center for Experimental Psychology Education, Beijing Normal University, China. Electronic address:

Objective: The belief in upward social mobility promotes the mental health and social adaptation of disadvantaged adolescents. However, efforts for upward mobility may facilitate psychosocial adaptation while undermining physical health for minority youth from disadvantaged backgrounds. Therefore, we aimed to examine how social mobility belief is associated with mental and physical health among Chinese rural-to-urban migrant adolescents and explore whether positive affect acts as a protective factor moderating these relationships.

Method: A total of 158 rural-to-urban migrant adolescents aged 10-14 completed a self-report questionnaire and underwent medical examination.

Results: Social mobility belief and positive affect were positively related to mental health (general self-efficacy). Positive affect interacted with social mobility belief in predicting physical health (self-rated health and allostatic load). For migrant adolescents with low levels of positive affect, strong belief in social mobility was associated with poor self-rated health and high allostatic load. For migrant adolescents with high levels of positive affect, their physical health was better and not associated with social mobility belief.

Conclusions: Social mobility belief showed a double-edged sword effect; it was positively associated with mental adaptation while negatively associated with physical health among Chinese rural-to-urban migrant adolescents. Positive affect acted as a protective factor for decreasing the negative association between social mobility belief and physical health.
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http://dx.doi.org/10.1016/j.psyneuen.2022.105846DOI Listing
June 2022

Sequence-Dependent Tyrosine-Containing Peptide Nanoassemblies for Sensing Tyrosinase and Melanoma.

ACS Macro Lett 2022 Jun 28:875-881. Epub 2022 Jun 28.

Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer-Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Tyrosine-containing peptide nanoassemblies have received tremendous attention because of their potential applications in biomedicine and nanomaterial fields. However, a current outstanding challenge is to direct the equilibrium between oxidative polymerization of precursors and the noncovalent assembly to precisely tune their specific nanostructures and functionalities through the rational design of peptide sequences. With a simple library of tripeptides containing tyrosine, glycine, and lysine, here we demonstrate how amino acid sequence encodes the property of tripeptide nanoassemblies by modulating the enzymatic oxidation of tyrosinase with the accompanied self-assembly, and thus select the pathways toward fluorescent or melanin-like nanoassemblies. The fluorescence of tripeptide nanoassemblies has been demonstrated in sensing both tyrosinase and melanoma. Our findings will provide inspiration of peptide sequence design for generating the complex bioactive peptide nanomaterials for biomedical applications.
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http://dx.doi.org/10.1021/acsmacrolett.2c00180DOI Listing
June 2022

DPA-Substituted Carbazole Derivative as a Fluorescent Ligand for G4 DNA.

Chem Biodivers 2022 Jun 28:e202200061. Epub 2022 Jun 28.

School of Pharmacy, Jiangsu University, Zhenjiang, 212013, P. R. China.

Herein a conjugated dipicolylamine/carbazole (Car-DPA) molecule was designed and synthesized to enhance the performance for the application as a G4 fluorescent ligand. This ligand has been found to display distinct and specific fluorescence enhancements in the presence of various G4 DNA structures, but limited with ssDNA or dsDNAs. The detail binding characteristics of the ligand with c-MYC G4 DNA were investigated by fluorescence, UV/VIS absorption, CD spectroscopy, and molecular docking. The present study demonstrated that Car-DPA bound to c-MYC G4s with a two-step complex formation, in which the binding mode appeared to be end-stacking. Confocal fluorescence images indicated that ligand Car-DPA could locate in nucleus, which is quite prominent from the cellular internalization studies.
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http://dx.doi.org/10.1002/cbdv.202200061DOI Listing
June 2022

Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.

Cell Commun Signal 2022 Jun 27;20(1):98. Epub 2022 Jun 27.

School of Clinical Medicine, Chengdu Medical College, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People's Republic of China.

Background: Aspergillus fumigatus infection is difficult to diagnose clinically and can develop into invasive pulmonary aspergillosis, which has a high fatality rate. The incidence of Aspergillus fumigatus infection has increased die to widespread application of radiotherapy technology. However, knowledge regarding A. fumigatus infection following radiation exposure is limited, and the underlying mechanism remains unclear. In this study, we established a mouse model to explore the effect of radiation on A. fumigatus infection and the associated mechanisms.

Methods: In this study, a mouse model of A. fumigatus infection after radiation was established by irradiating with 5 Gy on the chest and instilling 5 × 10/ml Aspergillus fumigatus conidia into trachea after 24 h to explore the effect and study its function and mechanism. Mice were compared among the following groups: normal controls (CON), radiation only (RA), infection only (Af), and radiation + infection (RA + Af). Staining analyses were used to detect infection and damage in lung tissues. Changes in protein and mRNA levels of pyroptosis-related molecules were assessed by western blot analysis and quantitative reverse transcription polymerase chain reaction, respectively. Protein concentrations in the serum and alveolar lavage fluid were also measured. An immunofluorescence colocalization analysis was performed to confirm that NLRP3 inflammasomes activated pyroptosis.

Results: Radiation destroyed the pulmonary epithelial barrier and significantly increased the pulmonary fungal burden of A. fumigatus. The active end of caspase-1 and gasdermin D (GSDMD) were highly expressed even after infection. Release of interleukin-18 (IL-18) and interleukin-1β (IL-1β) provided further evidence of pyroptosis. NLRP3 knockout inhibited pyroptosis, which effectively attenuated damage to the pulmonary epithelial barrier and reduced the burden of A. fumigatus.

Conclusions: Our findings indicated that the activation of NLRP3 inflammasomes following radiation exposure increased susceptibility to A. fumigatus infection. Due to pyroptosis in lung epithelial cells, it resulted in the destruction of the lung epithelial barrier and further damage to lung tissue. Moreover, we found that NLRP3 knockout effectively inhibited the pyroptosis and reducing susceptibility to A. fumigatus infection and further lung damage. Overall, our results suggest that NLRP3/GSDMD pathway mediated-pyroptosis in the lungs may be a key event in this process and provide new insights into the underlying mechanism of infection. Video abstract.
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http://dx.doi.org/10.1186/s12964-022-00907-2DOI Listing
June 2022

GREPore-seq: A robust workflow to detect changes after gene editing through long-range PCR and nanopore sequencing.

Genomics Proteomics Bioinformatics 2022 Jun 22. Epub 2022 Jun 22.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. Electronic address:

To achieve the enormous potential of gene-editing technology in clinical therapies, one needs to evaluate both the on-target efficiency and unintended editing consequences comprehensively. However, there is a lack of a pipelined, large-scale, and economical workflow for detecting genome editing outcomes, in particular insertion or deletion of a large fragment. Here, we describe an approach for efficient and accurate detection of multiple genetic changes after CRISPR/Cas9 editing by pooled nanopore sequencing of barcoded long-range PCR products. Recognizing the high error rates of Oxford nanopore sequencing, we developed a novel pipeline to capture the barcoded sequences by grepping reads of nanopore amplicon sequencing (GREPore-seq). GREPore-seq can assess nonhomologous end-joining (NHEJ)-mediated double-stranded oligodeoxynucleotide (dsODN) insertions with comparable accuracy to Illumina next-generation sequencing (NGS). GREPore-seq also reveals a full spectrum of homology-directed repair (HDR)-mediated large gene knock-in, correlating well with the fluorescence-activated cell sorting (FACS) analysis results. Of note, we discovered low-level fragmented and full-length plasmid backbone insertion at the CRISPR cutting site. Therefore, we have established a practical workflow to evaluate various genetic changes, including quantifying insertions of short dsODNs, knock-ins of long pieces, plasmid insertions, and large fragment deletions after CRISPR editing. GREPore-seq is freely available at GitHub (https://github.com/lisiang/GREPore-seq) and the National Genomics Data Center (NGDC) BioCode (https://ngdc.cncb.ac.cn/biocode/tools/BT007293).
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http://dx.doi.org/10.1016/j.gpb.2022.06.002DOI Listing
June 2022

Amelioration of Coastal Salt-Affected Soils with Biochar, Acid Modified Biochar and Wood Vinegar: Enhanced Nutrient Availability and Bacterial Community Modulation.

Int J Environ Res Public Health 2022 Jun 14;19(12). Epub 2022 Jun 14.

Institute of Coastal Environmental Pollution Control, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, China.

As an important part of the ecological environment, degraded coastal soils urgently require efficient and eco-friendly soil amendment. Biochar and wood vinegar have been proved to be effective soil amendments, and acid-modified biochar has great potential in ameliorating the degraded coastal saline-alkali soil. However, the effects of individual or combined application of biochar (BC), acid-modified biochar (ABC), and wood vinegar (WV) on coastal saline-alkali soil are unknown. Hence, biochar, wood vinegar, and acid-modified biochar were prepared by pyrolysis of poplar wood. The properties of biochar were characterized, and soil incubation experiments were conducted. The results showed that ABC decreased the soil alkalinity by acid-base neutralization and improved the soil fertility by increasing the nutrients (C, N, P). ABC provided a more suitable environment and changed the abundance and diversity of soil microorganisms. ABC increased the relative contents of specific families (e.g., and ), which had strong ecological linkages in the C, N, and P cycles and organic matter degradation. The results indicated that WV had little effect on coastal saline-alkali soil, whereas individual and combined application of biochar (especially ABC) showed an efficient remediation effect. Our preliminary study demonstrated that the ABC could be a suitable solution for ameliorating degraded coastal saline-alkali soils.
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http://dx.doi.org/10.3390/ijerph19127282DOI Listing
June 2022

Correlation between Electrode Location and Anxiety Depression of Subthalamic Nucleus Deep Brain Stimulation in Parkinson's Disease.

Brain Sci 2022 Jun 8;12(6). Epub 2022 Jun 8.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Objectives: our group explored the correlation between postoperative coordinates of the electrode contacts, VTA, and anxiety and depression symptoms in Parkinson's disease (PD) patients after subthalamic nucleus deep brain stimulation (STN-DBS).

Methods: STN-DBS was conducted on PD patients (n = 57) for six months with follow-up. Clinical outcomes were explored using the unified Parkinson's disease rating scale Part III (UPDRS-III), the Hamilton Anxiety Rating Scale (HAM-A), and the Hamilton Depression Rating Scale (HAM-D) before and after surgery. At the Montreal Neurological Institute (MNI), the location of active contacts and the volume of tissue activated (VTA) were calculated.

Results: patient evaluations took place preoperatively and follow-ups took place at 1 month, 3 months, and 6 months. The average patient improvement rates for HAM-A and HAM-D scores at the 6-month follow-up were 41.7% [interquartile range (IQR) 34.9%] and 37.5% (IQR 33.4%), respectively (both < 0.001). In medication-off, there were negative correlations between the HAM-A improvement rate and the Z-axis coordinate of the active contact (left side: = -0.308, = 0.020; right side: = -0.390, = 0.003), and negative correlations between the HAM-D improvement rate and the Z-axis coordinate of the active contact (left side: = -0.345, = 0.009; right side: = -0.521, = 0.001). There were positive correlations between the HAM-A and HAM-D scores improvement rate at 6 months after surgery and bilateral VTA in the right STN limbic subregion (HAM-A: = 0.314, = 0.018; HAM-D: = 0.321, = 0.015).

Conclusion: bilateral STN-DBS can improve anxiety and depression symptoms in PD patients. The closer the stimulation to the ventral limbic region of the STN, the more significant the improvement in anxiety and depression symptoms of PD patients.
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http://dx.doi.org/10.3390/brainsci12060755DOI Listing
June 2022

[Analysis of swelling stress and signaling pathway of human lung cancer cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 Jun;38(6):542-547

Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University, Xi'an 710038, China. *Corresponding authors, E-mail:

Objective To investigate whether swelling stress exists in lung cancer cells by comparing water content and nucleus size as well as mitogen activated protein kinase (MAPK) phosphorylation activation between lung cancer tissues and corresponding paracancerous tissues. Methods Differences in water content between fresh specimens of 10 human lung adenocarcinoma, 12 lung squamous cell carcinoma, and corresponding paracancerous normal lung tissues were examined through "dry and wet" mass comparison. The expressions and phosphorylation activation of mitogen activated protein kinases JNK, ERK1/2, and p38 in 30 lung adenocarcinoma samples, 32 lung squamous cell carcinoma samples, and alveolar epithelial cells and lung bronchial epithelial cells of 10 paracancerous normal tissues samples were detected with their phosphorylated and non-phosphorylated antibodies and by immunohistochemical staining. Nucleus size differences between cancer cells from lung adenocarcinoma and lung squamous cell carcinoma samples and corresponding paracancerous normal lung alveolar epithelial cells or bronchial epithelial cells were estimated by HE staining and analyzed with image analysis software. Results The average water content of lung squamous cell carcinoma and lung adenocarcinoma samples were significantly higher than that of their corresponding paracancerous normal tissues. Immunohistochemistry showed that p38 was highly expressed in all samples of lung adenocarcinoma, lung squamous cell carcinoma, and paracancerous tissues. ERK was weakly expressed in lung adenocarcinoma and squamous cell carcinoma samples, but highly expressed in paracancerous tissues. JNK was weakly expressed in lung adenocarcinoma and paracancerous tissues. JNK, p38 MAPK, and ERK1/2 were not activated through phosphorylation in alveolar or bronchial epithelial tissues. However, JNK, not p38 or ERK1/2, was phosphorylated in lung adenocarcinoma and lung squamous cell carcinoma tissues. The average nucleus size of lung cancer cells was significantly larger than those of their corresponding paracancerous normal lung epithelial cells. Conclusion There is swelling stress in lung cancer cells, and different types of human lung cancer cells have different swelling stress signaling pathways.
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June 2022

Emergence of extensive multidrug resistant Staphylococcus aureus carrying novel Sa-MRR in retail food.

J Glob Antimicrob Resist 2022 Jun 19. Epub 2022 Jun 19.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, state Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, PR China. Electronic address:

Objectives: The aim of this study was to investigate the prevalence and genetic environment of the multidrug resistance gene lsa(E) in food-related S. aureus in China.

Methods: 1463 S. aureus from retail food products in 39 Chinese cities were investigated to determined the prevalence of lsa(E). Furthermore, antimicrobial susceptibility testing, whole-genome sequencing (WGS) and complete genetic analysis were performed in lsa(E)-positive isolates.

Results: As a result, thirty-five isolates (2.4%) were positive for the lsa(E) gene which had an extensive multidrug-resistance phenotype. ST9-t899 and ST1-t4792 were the common types in positive strains. The lsa(E) genes were located in two different types of novel multiresistance region (MRR) on the chromosome. The Sa-MRR-I were inserted into lctP gene. The Sa-MRR-II were inserted into crtP gene and they were comprised of 7 ARGs interspersed with varieties of ISs, transposons and DNA invertase genes, showing is a novel arrangement harboring lsa(E). Part of transposon Tn1546 was inserted into downstream of lnu(B) in the novel Sa-MRR-II. Both two types of Sa-MRR could be excised from chromosome, indicating the Sa-MRR may be transferable.

Conclusion: Our study is the first systematical investigation of lsa(E)-positive S. aureus in retail foods in China. It indicated that the origin of most food-related lsa(E)-positive S. aureus in China might be associated with livestock or poultry breeding farm and has been transmitted between animal and food. Moreover, the emergence of S. aureus carrying novel Sa-MRR, especially serve as a reservoir of antibiotic resistance traits, should warrants further attention.
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http://dx.doi.org/10.1016/j.jgar.2022.06.011DOI Listing
June 2022

Comprehensive chromosome FISH assessment of sperm aneuploidy in normozoospermic males.

J Assist Reprod Genet 2022 Jun 22. Epub 2022 Jun 22.

Shanghai Ji Ai Genetics & IVF Institute, Shanghai, 200011, China.

Purpose: Sperm chromosomal abnormalities impact male fertility and pregnancy outcomes. However, the proportion of sperm with chromosomal abnormalities in normozoospermic men remains unclear. Herein, we evaluated sperm aneuploidy for 23 chromosomes to elucidate its incidence in normozoospermic men.

Methods: Sperm from ten normozoospermic donors were obtained from a human sperm bank and analyzed using fluorescence in situ hybridization. The frequencies of nullisomy, disomy, and diploidy were analyzed along with trisomy, triploidy, tetraploidy, and other numerical abnormalities per chromosome and per donor levels.

Results: A total of 248,811 sperm cells were analyzed (average: 24,881 ± 381 cells/donor), of which 246, 658 were haploid, 818 nullisomic, 393 disomic, 894 diploid, 13 triploid, 8 tetraploid, 3 trisomic, and 24 harbored multiple aneuploidies. Among the 22 autosomal and 2 sex chromosomes, the mean frequency of aneuploidy per chromosome was 0.49 ± 0.16%, including 0.33 ± 0.16% for nullisomy and 0.16 ± 0.08% for disomy. The mean frequencies of nullisomy, disomy, and aneuploidy per donor were 0.33 ± 0.13%, 0.16 ± 0.05%, and 0.49 ± 0.13%, respectively. The total frequencies of nullisomy, disomy, diploidy, and aneuploidy per donor were 7.62 ± 3.06%, 3.63 ± 1.12%, 0.36 ± 0.15%, and 11.25 ± 3.05%, respectively.

Conclusions: The dominant chromosome numerical abnormalities in normozoospermic men are nullisomy, disomy, and diploidy. Generally, the frequency of nullisomy is higher than that of disomy. The disomy or nullisomy frequencies for each chromosome being gained or lost were not unified and varied; some chromosomes (e.g., chromosomes 21 and 22 and sex chromosomes) are more prone to disomy while some others (e.g., chromosome 3) are more prone to nullisomy.
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http://dx.doi.org/10.1007/s10815-022-02536-7DOI Listing
June 2022

Genome-wide association studies in non-anxiety individuals identified novel risk loci for depression.

Eur Psychiatry 2022 Jun 22:1-40. Epub 2022 Jun 22.

Key Laboratory of Trace Elements and Endemic Diseases, Collaborative Innovation Center of Endemic Disease and Health Promotion for Silk Road Region, School of Public Health, Health Science Center, Xi'an Jiaotong University, 710061, P.R. China.

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http://dx.doi.org/10.1192/j.eurpsy.2022.32DOI Listing
June 2022

POMCPs with Novel Two Water-Assisted Proton Channels Accommodated by MXenes for Asymmetric Supercapacitors.

Small 2022 Jun 21:e2202087. Epub 2022 Jun 21.

Key Laboratory of Green Chemical Engineering and Technology, School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150040, P. R. China.

To develop high-performance supercapacitors, the negative electrode is at present viewed as one of the most challenging tasks for obtaining the next-generation of energy storage devices. Therefore, in this study, a polyoxometalate-based coordination polymer [Zn(itmb) H O][H SiW O ]·5H O (1) is designed and prepared by a simple hydrothermal method for constructing a high-capacity negative electrode. Polymer 1 has two water-assisted proton channels, which are conducive to enhancing the electrical conductivity and storage capacity. Then, MXene Ti C T is chosen to accommodate coordination polymer 1 as the interlayer spacers to improve the conductivity and cycling stability of 1, while preventing the restacking of MXene. Expectedly, the produced composite electrode [email protected] C T shows an excellent specific capacitance (1480.1 F g at 5 A g ) and high rate performance (a capacity retention of 71.5% from 5 to 20 A g ). Consequently, an asymmetric supercapacitor device is fabricated using [email protected] C T as the negative electrode and celtuce leaves-derived carbon paper as the positive electrode, which demonstrates ultrahigh energy density of 32.2 Wh kg , and power density 2397.5 W kg , respectively. In addition, the ability to illuminate a red light-emitting diode for several minutes validates its feasibility for practical application.
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http://dx.doi.org/10.1002/smll.202202087DOI Listing
June 2022

Polar Vessel: A New Ultrasound Sign for Complementary Diagnosis of Major Salivary Gland Adenoid Cystic Carcinoma.

J Ultrasound Med 2022 Jun 20. Epub 2022 Jun 20.

Department of Ultrasound, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Objectives: To investigate the characteristic ultrasonographic findings of adenoid cystic carcinoma (ACC) in major salivary glands and identify the value of polar vessel in color Doppler flow imaging (CDFI) for the diagnosis of ACC.

Methods: From January 2017 to December 2021, 76 patients with parotid and submandibular gland tumors, including 14 patients with ACC, as confirmed by surgery and histopathology, were enrolled. Their clinicopathologic information and ultrasound (US) features were recorded and analyzed. The performance of polar vessel in CDFI for differentiating ACC from non-ACC (benign tumors and mucoepidermoid carcinoma [MEC]) was analyzed.

Results: ACC in the major salivary gland was more likely to be associated with pain symptoms (P = .027) and unclear borders and rough edges in grayscale US (P = .002, .015, respectively) than benign tumors. Compared to MEC, ACC tended to feature a homogeneous internal echo (P = .008). ACC of the major salivary gland had a significantly higher incidence of polar vessel sign than that of non-ACC (benign tumors and MEC) (P < .0001, .0001, respectively). The polar vessel sign showed good performance in distinguishing between ACC and non-ACC, with an area under the receiver operating characteristic curve of 0.857, a sensitivity of 71.4%, a specificity of 100%, and an accuracy of 94.7%. Positive predictive value and negative predictive value were calculated at 100% and 93.9%, respectively.

Conclusions: The US sign of polar vessel has high diagnostic efficiency, and it may have important potential for use as a new complementary sign for the diagnosis of ACC in major salivary glands.
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http://dx.doi.org/10.1002/jum.16043DOI Listing
June 2022

Biological Efficacy Comparison of Natural Tussah Silk and Mulberry Silk Nanofiber Membranes for Guided Bone Regeneration.

ACS Omega 2022 Jun 31;7(23):19979-19987. Epub 2022 May 31.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, China.

Biopolymer nanofiber membranes are attracting interest as promising biomaterial scaffolds with a remarkable range of structural and functional performances for guided bone regeneration (GBR). In this study, tussah silk nanofiber (TSn) and silk nanofiber (BSn) membranes were prepared by physical shearing. The diameters of the TSn and BSn membranes were 146.09 ± 63.56 and 120.99 ± 91.32 nm, respectively. TSn showed a Young's modulus of 3.61 ± 0.64 GPa and a tensile strength of 74.27 ± 5.19 MPa, which were superior to those of BSn, with a Young's modulus of 0.16 ± 0.03 GPa and a tensile strength of 4.86 ± 0.61 MPa. The potential of TSn and BSn membranes to guide bone regeneration was explored. In vitro, the TSn membrane exhibited significantly higher cell proliferation for MC3T3-E1 cells than the BSn membrane. In a cranial bone defect in a rat model, the TSn and BSn membranes displayed superior bone regeneration compared to the control because the membrane prevented the ingrowth of soft tissue to the defective area. Compared to the BSn membrane, the TSn membrane improved damaged bone regeneration, presumably due to its superior mechanical properties, high osteoconductivity, and increased cell proliferation. The TSn membrane has a bionic structure, excellent mechanical properties, and greater biocompatibility, making it an ideal candidate for GBR.
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http://dx.doi.org/10.1021/acsomega.2c01784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202271PMC
June 2022

Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients.

Front Cell Dev Biol 2022 2;10:916961. Epub 2022 Jun 2.

Department of Nutrition, Affiliated Hospital of Jiangnan University, Wuxi, China.

Association studies have linked microbiome alterations with colorectal cancer (CRC). However, differences in tumor, para-cancerous, normal mucosal, and fecal microbiota remain to be strengthened. We performed a study on the ecologically rich and taxonomically diverse of gut microbiota using three types of colorectal mucosa (tumor mucosa, para-cancerous mucosa, normal mucosa) and feces from 98 CRC patients. Additionally, we profiled the microbiota in the fecal occult blood test (FOBT) positive and negative groups at different sampling sites. We found striking variations between tumor mucosal microbiota and normal mucosal microbiota. However, there was no significant difference between tumor and para-cancerous mucosal microbiota, as well as between para-cancerous and normal mucosal microbiota, revealing that the para-cancerous mucosal microbiota was a transitional state between the tumor and normal mucosal microbiota. And the substantial shifts in the fecal microbiota compared to mucosal microbiota indicated the risk of using fecal microbiota to define mucosal microbiota. A strong correlation between FOBT positive and was discovered, indicating this adherent-invasive genus was closely related to intestinal bleeding. Furthermore, we identified six key genera, including , , , , , and , which appear to be consistently over-represented in tumor mucosa compared to normal mucosa and/or in mucosa compared to feces. Compositional alterations in the microbiota existed in three types of colorectal mucosa and feces in CRC patients. Six key genera may contribute to the topographic variances in the microbiota of tumor-bearing colorectum.
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http://dx.doi.org/10.3389/fcell.2022.916961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201480PMC
June 2022

Aqueous Extract of Guava ( L.) Leaf Ameliorates Hyperglycemia by Promoting Hepatic Glycogen Synthesis and Modulating Gut Microbiota.

Front Pharmacol 2022 1;13:907702. Epub 2022 Jun 1.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, Metabolic Syndrome Research Center, Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Type 2 diabetes mellitus (T2DM) is a major global health concern. L. (guava) is widely used for food as well as a folk medicine. Previous studies have shown its anti-diabetic and anti-inflammatory properties. However, the underlying mechanisms remains to be elusive. In this study, we assessed the potential therapeutic effects of aqueous extract of guava leaves (GvAEx) on T2DM and explored their potential mechanisms and . GvAEx was gavage administered for 12 weeks in diabetic db/db mice. Our results have demonstrated that GvAEx significantly lowered fasting plasma glucose levels ( < 0.01) and improved glucose tolerance and insulin sensitivity ( < 0.01, < 0.05, respectively). Additionally, GvAEx increased hepatic glycogen accumulation, glucose uptake and decreased the mRNA expression levels of gluconeogenic genes. Furthermore, GvAEx-treatment caused higher glucose transporter 2 (GLUT2) expression in the membrane in hepatocytes. Notably, for the first time, we have elaborated the possible mechanism of the hypoglycemic effect of GvAEx from the perspective of intestinal microbiota. GvAEx has significantly changed the composition of microbiota and increased short chain fatty acid (SCFA) -producing Lachnospiraceae family and genus in the gut. Taken together, GvAEx could alleviate hyperglycemia and insulin resistance of T2DM by regulating glucose metabolism in the liver and restoring the gut microbiota. Thus, GvAEx has the potential for drug development against T2DM.
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http://dx.doi.org/10.3389/fphar.2022.907702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198539PMC
June 2022

Integrated Chemical Interpretation and Network Pharmacology Analysis to Reveal the Anti-Liver Fibrosis Effect of .

Front Pharmacol 2022 2;13:788388. Epub 2022 Jun 2.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine (SHUTCM), Shanghai, China.

Liver fibrosis is a disease with complex pathological mechanisms. Pursh (e) is a traditional Chinese medicine (TCM) for liver injury treatment. However, the pharmacological mechanisms of e on liver fibrosis have not been investigated and clarified clearly. This study was designed to investigate the chemicals in e and explore its effect on liver fibrosis. First, we developed a highly efficient method, called DDA-assisted DIA, which can both broaden mass spectrometry (MS) coverage and MS quality. In DDA-assisted DIA, data-dependent acquisition (DDA) and data-independent acquisition (DIA) were merged to construct a molecular network, in which 1,094 mass features were retained in Pursh (e). Out of these, 169 compounds were identified based on both MS and MS analysis. After that, based on a network pharmacology study, 94 bioactive compounds and 440 targets of e associated with liver fibrosis were obtained, forming a tight compound-target network. Meanwhile, the network pharmacology experimental results showed that multiple pathways interacted with the HIF-1 pathway, which was first identified involved in e. It could be observed that some proteins, such as TNF-α, Timp1, and HO-1, were involved in the HIF-1 pathway. Furthermore, the pharmacological effects of on these proteins were verified by CCl-induced rat liver fibrosis, and was found to improve liver functions through regulating TNF-α, Timp1, and HO-1 expressions. In summary, DDA-assisted DIA could provide more detailed compound information, which will help us to annotate the ingredients of TCM, and combination with computerized network pharmacology provided a theoretical basis for revealing the mechanism of .
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http://dx.doi.org/10.3389/fphar.2022.788388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201443PMC
June 2022

The Relationship Between Chinese College Student Offspring's Physical Activity and Father Physical Activity During COVID-19 Pandemic.

Front Public Health 2022 3;10:896087. Epub 2022 Jun 3.

Department of Electrical and Computer Engineering (DEEC), Institute of Systems and Robotics (ISR), University of Coimbra, Coimbra, Portugal.

The purpose of this study was to evaluate the relationship between the physical activity of Chinese college students and the physical activity of their parents. This relationship was examined by linear regression. The results showed that (1) the physical activities of China's college students will be refurbished and used for the parents. The offspring of COVID-19's later generation sports population showed that the offspring's physical activity was not broken during the later stage of the epidemic, and the coefficient of promotion ( = 1.515) ( < 0.01) of the offspring's participation in the physical activities was. (2) The increase or decrease of parents' sports population is affected by their children's occupation. Therefore, it shows the dynamic role of individuals and the two-way nature of socialization in the process of socialization. With the transformation from traditional society to modern society, promoting college students' physical activities can increase parents' physical activities and improve the level of social physical activities.
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http://dx.doi.org/10.3389/fpubh.2022.896087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204172PMC
June 2022

Materialism Predicts College Students' Entrepreneurial Intention: A Serial Mediation Model.

Front Psychol 2022 2;13:864069. Epub 2022 Jun 2.

School of Economics and Management, North China Electric Power University, Beijing, China.

Entrepreneurship is perceived as a critical pillar for unemployment alleviation and economic growth, especially in the era of COVID-19, which highlights the importance of the entrepreneurial potential of college students. The current research focused on the role of personal values in the entrepreneurial process and investigated the relationship between materialism and entrepreneurial intention among college students. Few studies have been examined this relationship, and the underlying mechanisms were also not identified. From the perspective of personal value, we hypothesized that materialism could positively predict entrepreneurial intention. Moreover, based on McClelland's theory of need for achievement and the theory of planned behavior, a serial mediation model, with achievement motivation and entrepreneurial attitude as the mediators, was proposed. We conducted a correlational study on a sample of 1,002 Chinese university students to examine our hypotheses. They participated in an online survey and completed the measurement of entrepreneurial intention, entrepreneurial attitude, materialism, and achievement motivation. The hypothesized models were examined through serial mediation bootstrapping procedures. The results showed that materialism positively predicted college students' entrepreneurial intention, and this relationship was serially mediated through achievement motivation and entrepreneurial attitude. Materialism boosted college students' achievement motivation, which in turn was associated with a more positive entrepreneurial attitude and subsequently stronger entrepreneurial intention. The present research is the first to empirically examine this association's mechanism and establish a serial mediation involving achievement motivation and entrepreneurial attitude. For the theoretical contribution, the present research provides a more comprehensive picture of the role of personal values in entrepreneurship by complementing the effect of materialism. And regarding the practical implications, the present research implies the silver lining of materialism and points out a possible way to enhance college students' entrepreneurial intention, i.e., entrepreneurial education could take advantage of the characteristics of materialism and transform the "harmful" value into socially beneficial entrepreneurial intentions through enhancing their achievement motivation and positive attitude toward entrepreneurship.
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http://dx.doi.org/10.3389/fpsyg.2022.864069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201413PMC
June 2022

Development of a novel method for the continuous blending of carrier-based dry powders for inhalation using a co-rotating twin-screw extruder.

Int J Pharm 2022 Jun 16;623:121914. Epub 2022 Jun 16.

Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, The University of Texas at Austin, 2409 University Avenue, A1920 Austin, TX 78712, USA. Electronic address:

Twin-screw extruders are useful in tuning certain product characteristics due to the ability to greatly modify screw profiles as well as operating parameters. However, their use has not yet been applied to dry powder inhalation. In this study the feasibility of using a twin-screw extruder to blend dry powders for inhalation was assessed. Micronized rifampicin (1%) was used as a model drug with lactose carrier (median size ∼ 44 µm) and 0.4% magnesium stearate as a multi-functional ternary agent. Blend performance was compared with low shear (Turbula®) batch mixing. Similar blend uniformity and aerosol performance was observed, indicating the twin-screw extruder successfully functions as a mixer for dry powders for inhalation. The ability to utilize the twin-screw extruder as a continuous mixer leads to new opportunities in the continuous manufacturing of powders for inhalation.
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http://dx.doi.org/10.1016/j.ijpharm.2022.121914DOI Listing
June 2022

Magnetic graphene oxide-based covalent organic frameworks as novel adsorbent for extraction and separation of triazine herbicides from fruit and vegetable samples.

Anal Chim Acta 2022 Aug 24;1219:339984. Epub 2022 May 24.

Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China; Key Laboratory of Food Quality and Safety for State Market Regulation, Beijing, 100176, China. Electronic address:

A novel magnetic graphene oxide-based covalent organic frameworks, was prepared initially by grafting two monomers, tris(4-aminophenyl)amine and 2, 4, 6-triformylphloroglucinol onto FeO nanoparticles attached to graphene oxide scaffolds. It exhibited a regular core-shell spherical structure, super paramagnetism, and desirable adsorbability for five triazine herbicides, and was thus served as adsorbent of magnetic solid-phase extraction. An analytical strategy coupled magnetic solid phase extraction with UHPLC-MS/MS was then developed for the determination of triazine herbicide residues in fruit and vegetable samples. The validation results revealed good linearity in the concentration range of 0.01-200 μg L(r0.9925). LODs of the method were in the range of 0.01-0.05 μg kg, LOQs in the range of 0.05-0.2 μg kg and recoveries ranged from 72.3 to 112.8%. The results showed that the prepared adsorbent provided desirable adsorption capacity of triazine herbicides, demonstrating great potential in the detection of trace triazine herbicides in complex food matrices.
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http://dx.doi.org/10.1016/j.aca.2022.339984DOI Listing
August 2022

RUNX3-regulated circRNA METTL3 inhibits colorectal cancer proliferation and metastasis via miR-107/PER3 axis.

Cell Death Dis 2022 Jun 16;13(6):550. Epub 2022 Jun 16.

National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, P.R. China.

Colorectal cancer (CRC) is one of the most prevalent and lethal malignancies. Exploring the underlying molecular mechanisms is very helpful for the development of new therapy. Here, we investigated the function of circMETTL3/miR-107/PER3 in CRC. Human CRC tissues from diagnosed CRC patients and six CRC cell lines, one normal human colon cell line were used. qRT-PCR and western blotting were performed to determine expression levels of RUNX3, circMETTL3, miR-107, PER3, and proliferation-, and migration-related proteins. CCK-8, colony formation assay, transwell assay, and scratch wound assay were utilized to assess CRC cell proliferation and invasion. ChIP, EMSA, biotin-pull down, RIP assay, and dual luciferase reporter assay were performed to validate interactions of RUNX3/METTL3 promoter, circMETTL3/miR-107, and miR-107/PER3. FISH was used to characterize circMETTL3. MSP was employed to measure methylation level. Nude mouse xenograft model was used to determine the effects on tumor growth and metastasis in vivo. RUNX3, circMETTL3, and PER3 were diminished while miR-107 was elevated in CRC tissues and cells. Low levels of RUNX3 and circMETTL3 correlated with poor prognosis of CRC. Overexpression of RUNX3, circMETTL3, or PER3 suppressed while miR-107 mimics promoted, CRC cell proliferation and invasion, as well as tumor growth and metastasis in vivo. Mechanistically, RUNX3 bound to METTL3 promoter and activated circMETTL3 transcription. circMETTL3 directly bound with miR-107 which targeted PER3. circMETTL3/miR-107 regulated CRC cell proliferation and invasion via PER3. CircMETTL3, transcriptionally activated by RUNX3, restrains CRC development and metastasis via acting as a miR-107 sponge to regulate PER3 signaling.
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http://dx.doi.org/10.1038/s41419-022-04750-8DOI Listing
June 2022

PBP1 plays key roles in sex pheromone reception of the fall armyworm.

Int J Biol Macromol 2022 Jun 14;214:162-169. Epub 2022 Jun 14.

Anhui-CABI Joint Laboratory for Agricultural Pest Control, Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China. Electronic address:

Spodoptera frugiperda among the China population employs a four-component sex pheromone blend to accelerate male-female allocation and mating behavior. The underlying molecular mechanism has been incompletely elucidated. In the current study, we showed that differences existed between genders toward the four sex pheromone components, including Z9-14:AC, Z7-12:AC, Z9-12:AC, and Z11-16:AC, in terms of electrophysiological responses and behavioral valences. Male adults were significantly more sensitive to all tested compounds than female adults. Furthermore, ecological outputs may be related to four pheromone-binding proteins, namely, SfruPBP1, SfruPBP2, SfruPBP3, and SfruPBP4. They formed four distinct clades within the lepidopteran phylogeny, and male adults expressed significantly higher levels of SfruPBP1 and SfruPBP2 than female adults. We observed the highest binding affinities of SfruPBP1 toward all four sex pheromone components. SfruPBP4 had moderate binding affinities for Z7-12:AC, Z11-16:AC, and Z9-12:AC, while SfruPBP2 showed binding toward Z9-14:AC. This observation suggests that SfruPBP1 plays a key role in sex pheromone discrimination and drives sexually biased behavioral decisions toward certain pheromone components. These findings will help to develop behavioral-mediating tools as part of integrated pest management approaches for this cross-border pest.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.068DOI Listing
June 2022

Homozygous variant in KASH5 causes premature ovarian insufficiency by disordered meiotic homologous pairing.

J Clin Endocrinol Metab 2022 Jun 16. Epub 2022 Jun 16.

Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.

Context: Premature ovarian insufficiency (POI) affects 1%-3.7% of women at reproductive age, which etiology is heterogeneous. The LINC (linker of nucleoskeleton and cytoskeleton) complex, consisting of KASH5 and SUN1, plays an indispensable role in meiotic homolog pairing, determining the ovarian reserve. However, their roles in the pathogenesis of POI are unknown.

Objective: To investigate the role of KASH5 variation in the pathogenesis of POI.

Design: Whole exome sequencing (WES) was performed in a pedigree with two POI patients. The pathogenicity of identified variant was illustrated by In vitro functional studies, and its effect on ovarian function and meiosis was confirmed by histological analysis and oocyte spreads with Kash5 C-terminal deleted mice model.

Results: A homozygous splicing site variant in KASH5 (c.747G>A) was identified. In vitro studies found the variant disturbed the nuclear membrane localization of KASH5 and its binding with SUN1. Moreover, the Kash5 C-terminal deleted mice revealed defective meiotic homolog pairing and accelerated depletion of oocytes.

Conclusions: The splicing site variant in KASH5 is responsible for POI due to defective meiotic homolog pairing and accelerated depletion of oocytes. Our study is the first to report disorganized LINC complex participating in POI pathogenesis, potentially suggesting the essential roles of meiotic telomere attachment and dynein-driven proteins for chromosome movement in ovarian function maintenance.
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http://dx.doi.org/10.1210/clinem/dgac368DOI Listing
June 2022

Tolerance of Senecavirus A to Mutations in Its Kissing-Loop or Pseudoknot Structure Computationally Predicted in 3' Untranslated Region.

Front Microbiol 2022 30;13:889480. Epub 2022 May 30.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.

Senecavirus A (SVA) is an emerging virus that belongs to the genus in the family . Its genome is a positive-sense and single-stranded RNA, containing two untranslated regions (UTRs). The 68-nt-long 3' UTR is computationally predicted to possess two higher-order RNA structures: a kissing-loop interaction and an H-type-like pseudoknot, both of which, however, cannot coexist in the 3' UTR. In this study, we constructed 17 full-length SVA cDNA clones (cD-1 to -17): the cD-1 to -7 contained different point mutations in a kissing-loop-forming motif (KLFM); the cD-8 to -17 harbored one single or multiple point mutations in a pseudoknot-forming motif (PFM). These 17 mutated cDNA clones were independently transfected into BSR-T7/5 cells for rescuing recombinant SVAs (rSVAs), named rSVA-1 to -17, corresponding to cD-1 to -17. The results showed that the rSVA-1, -2, -3, -4, -5, -6, -7, -9, -13, and -15 were successfully rescued from their individual cDNA clones. Moreover, all mutated motifs were genetically stable during 10 viral passages . This study unveiled viral abilities of tolerating mutations in the computationally predicted KLFM or PFMs. It can be concluded that the putative kissing-loop structure, even if present in the 3' UTR, is unnecessary for SVA replication. Alternatively, if the pseudoknot formation potentially occurs in the 3' UTR, its deformation would have a lethal effect on SVA propagation.
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http://dx.doi.org/10.3389/fmicb.2022.889480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189406PMC
May 2022

Hypoxia regulates fibrosis-related genes via histone lactylation in the placentas of patients with preeclampsia.

J Hypertens 2022 Jun;40(6):1189-1198

Reproductive Medicine Center, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Background: Histone lactylation, a novel epigenetic modification induced by hypoxia and lactate, plays an important role in regulating gene expression. However, the role of histone lactylation in the pathogenesis of preeclampsia remains unknown.

Methods: Placentas from preeclamptic patients and control pregnant women were collected for protein immunoassay to detect the expression level of histone lactylation, and two trophoblast cell lines were used to simulate the effect of histone lactylation on genes.

Results: We found that lactate and histone lactylation levels were increased in preeclamptic placentas. In vitro, hypoxia was demonstrated to induce histone lactylation by promoting the production of lactate in human-trophoblast-derived cell line (HTR-8/SVneo) and human first-trimester extravillous trophoblast cell line (TEV-1) cells. In addition, 152 genes were found to be upregulated by both hypoxia exposure and sodium l-lactate treatment in HTR-8/SVneo cells. These genes were mainly enriched in the pathways including the response to hypoxia, cell migration and focal adhesion. Among the 152 genes, nine were upregulated in preeclamptic placentas. Most noteworthy, two upregulated fibrosis-related genes, FN1 and SERPINE1, were promoted by hypoxia through histone lactylation mediated by the production of lactate.

Conclusions: The present study demonstrated the elevated levels of histone lactylation in preeclamptic placentas and identified fibrosis-related genes that were promoted by histone lactylation induced by hypoxia in trophoblast cells, which provides novel insights into the mechanism of placental dysfunction in preeclampsia.
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http://dx.doi.org/10.1097/HJH.0000000000003129DOI Listing
June 2022

Risk Factors Associated With Misdiagnosis of Digital Glomus Tumor: A Retrospective Cohort Study.

Ann Plast Surg 2022 Jun 11. Epub 2022 Jun 11.

Department of Orthopedics (Division of Hand Surgery), The Second Affiliated Hospital and Yuying Children' Hospital of Wenzhou Medical University, Key Laboratory of Orthopedics of Zhejiang Province, The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.

Objective: Glomus tumors are benign with unique triad of symptoms; however, the delayed diagnosis of these tumors is common. We investigated the possible risk factors for the misdiagnosis of digital glomus tumors, with an aim to treat these patients on time.

Methods: We conducted a retrospective cohort study of 104 patients with digital glomus tumors from October 2009 to February 2021. Data pertaining to sex, age, tumor locations, symptoms, imaging modalities, and clinical departments visited by the patients were extracted and analyzed through logistic regression.

Results: The duration of delayed diagnosis ranged from 3 months to 40 years (mean, 5.5 ± 6.5 years). The total misdiagnosis and recurrence rate are 34.6% and 3.8%, respectively. On the multivariate logistic regression, the misdiagnosis of digital glomus tumor was significantly associated with the clinical departments visited by the patients (P < 0.001). The risk of misdiagnosis of nonhand surgery department visit is 179.741-fold higher than that of hand surgery department visit.

Conclusions: The misdiagnosis rate of digital glomus tumor was closely related to the clinical departments visited by the patients. Hand surgeons are the first choice for the treatment of the tumor.
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http://dx.doi.org/10.1097/SAP.0000000000003263DOI Listing
June 2022

Epigenetic features drastically impact CRISPR-Cas9 efficacy in plants.

Plant Physiol 2022 Jun 11. Epub 2022 Jun 11.

Department of Plant and Microbial Biology, University of Minnesota, Saint Paul, MN 55108.

CRISPR-Cas9-mediated genome editing has been widely adopted for basic and applied biological research in eukaryotic systems. While many studies consider DNA sequences of CRISPR target sites as the primary determinant for CRISPR mutagenesis efficiency and mutation profiles, increasing evidence reveals the substantial role of chromatin context. Nonetheless, most prior studies are limited by the lack of sufficient epigenetic resources and/or by only transiently expressing CRISPR-Cas9 in a short time window. In this study, we leveraged the wealth of high-resolution epigenomic resources in Arabidopsis (Arabidopsis thaliana) to address the impact of chromatin features on CRISPR-Cas9 mutagenesis using stable transgenic plants. Our results indicated that DNA methylation and chromatin features could lead to substantial variations in mutagenesis efficiency by up to 250-fold. Low mutagenesis efficiencies were mostly associated with repressive heterochromatic features. This repressive effect appeared to persist through cell divisions but could be alleviated through substantial reduction of DNA methylation at CRISPR target sites. Moreover, specific chromatin features, such as H3K4me1, H3.3, and H3.1, appear to be associated with significant variation in CRISPR-Cas9 mutation profiles mediated by the non-homologous end joining repair pathway. Our findings provide strong evidence that specific chromatin features could have substantial and lasting impacts on both CRISPR-Cas9 mutagenesis efficiency and DNA double strand break repair outcomes.
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http://dx.doi.org/10.1093/plphys/kiac285DOI Listing
June 2022
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