Publications by authors named "Feng Yang"

1,950 Publications

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Salicylic acid-activated BIN2 phosphorylation of TGA3 promotes Arabidopsis PR gene expression and disease resistance.

EMBO J 2022 Aug 11:e110682. Epub 2022 Aug 11.

Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, China.

The plant defense hormone, salicylic acid (SA), plays essential roles in immunity and systemic acquired resistance. Salicylic acid induced by the pathogen is perceived by the receptor nonexpressor of pathogenesis-related genes 1 (NPR1), which is recruited by TGA transcription factors to induce the expression of pathogenesis-related (PR) genes. However, the mechanism by which post-translational modifications affect TGA's transcriptional activity by salicylic acid signaling/pathogen infection is not well-established. Here, we report that the loss-of-function mutant of brassinosteroid insensitive2 (BIN2) and its homologs, bin2-3 bil1 bil2, causes impaired pathogen resistance and insensitivity to SA-induced PR gene expression, whereas the gain-of-function mutant, bin2-1, exhibited enhanced SA signaling and immunity against the pathogen. Our results demonstrate that salicylic acid activates BIN2 kinase, which in turn phosphorylates TGA3 at Ser33 to enhance TGA3 DNA binding ability and NPR1-TGA3 complex formation, leading to the activation of PR gene expression. These findings implicate BIN2 as a new component of salicylic acid signaling, functioning as a key node in balancing brassinosteroid-mediated plant growth and SA-induced immunity.
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http://dx.doi.org/10.15252/embj.2022110682DOI Listing
August 2022

Folic Acid Enables Targeting Delivery of Lipodiscs by Circumventing IgM-Mediated Opsonization.

Nano Lett 2022 Aug 9. Epub 2022 Aug 9.

Center of Medical Research and Innovation, Shanghai Pudong Hospital & Department of Pharmacology, School of Basic Medical Sciences & State Key Laboratory of Molecular Engineering of Polymers, Fudan University Shanghai Engineering Research Center for Synthetic Immunology, Shanghai 201399, P.R. China.

Folic acid (FA) is one of the most widely utilized small-molecule ligands for cancer targeted drug delivery. Natural IgM was recently found to avidly absorb on the surface of FA-functionalized liposomes (FA-sLip), negatively regulating the performance by efficiently activating complement. Herein, FA-functionalized lipodiscs (FA-Disc) were constructed to successfully circumvent IgM-mediated opsonization and retained binding activity with folate receptors . The FA moiety along with the bound IgM was restricted to the highly curved rim of lipodiscs, leading to IgM incapability of presenting the membrane-bound conformation to trigger complement activation. The C1q docking, C3 binding, and C5a release were blocked and accelerated blood clearance phenomenon was mitigated of FA-Disc. FA-Disc retained folate binding activity and could effectively target folate receptor positive tumors . The present study provides a useful solution to avoid the negative regulation by IgM and achieve FA-enabled targeting by exploring disc-shaped nanocarriers.
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http://dx.doi.org/10.1021/acs.nanolett.2c01509DOI Listing
August 2022

miR-196b-TLR7/8 signaling axis regulates innate immune signaling and myeloid maturation in DNMT3A-mutant AML.

Clin Cancer Res 2022 Aug 9. Epub 2022 Aug 9.

Thomas Jefferson University, Philadelphia, PA, United States.

Purpose: DNMT3A mutations confer a poor prognosis in acute myeloid leukemia (AML), but the molecular mechanisms downstream of DNMT3A mutations in disease pathogenesis are not completely understood, limiting targeted therapeutic options. The role of microRNA in DNMT3A-mutant AML pathogenesis is understudied.

Experimental Design: DNA methylation and miRNA expression was evaluated in human AML patient samples and in Dnmt3a/Flt3-mutant AML mice. The treatment efficacy and molecular mechanisms of TLR7/8-directed therapies on DNMT3A-mutant AML were evaluated in vitro on human AML patient samples and in Dnmt3a/Flt3-mutant AML mice.

Results: miR-196b is hypomethylated and overexpressed in DNMT3A-mutant AML and is associated with poor patient outcome. miR-196b overexpression in DNMT3A-mutant AML is important to maintain an immature state and leukemic cell survival through repression of TLR signaling. The TLR7/8 agonist Resiquimod induces dendritic cell-like differentiation with co-stimulatory molecule expression in DNMT3A-mutant AML cells and provides a survival benefit to Dnmt3a/Flt3-mutant AML mice. The small molecule Bryostatin-1 augments Resiquimod-mediated AML growth inhibition and differentiation.

Conclusions: DNMT3A loss-of-function mutations cause miRNA locus-specific hypomethylation and overexpression important for mutant DNMT3A-mediated pathogenesis and clinical outcomes. Specifically, the overexpression of miR-196b in DNMT3A-mutant AML creates a novel therapeutic vulnerability by controlling sensitivity to TLR7/8-directed therapies.
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http://dx.doi.org/10.1158/1078-0432.CCR-22-1598DOI Listing
August 2022

Development of a Novel Prognostic Model of Glioblastoma Based on m6A-Associated Immune Genes and Identification of a New Biomarker.

Front Oncol 2022 20;12:868415. Epub 2022 Jul 20.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Accumulating evidence shows that m6A regulates oncogene and tumor suppressor gene expression, thus playing a dual role in cancer. Likewise, there is a close relationship between the immune system and tumor development and progression. However, for glioblastoma, m6A-associated immunological markers remain to be identified.

Methods: We obtained gene expression, mutation, and clinical data on glioblastoma from The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases. Next, we performed univariate COX-least absolute shrinkage and selection operator (LASSO)-multivariate COX regression analyses to establish a prognostic gene signature and develop a corresponding dynamic nomogram application. We then carried out a clustering analysis twice to categorize all samples according to their m6A-regulating and m6A-associated immune gene expression levels (high, medium, and low) and calculated their m6A score. Finally, we performed quantitative reverse transcription-polymerase chain reaction, cell counting kit-8, cell stemness detection, cell migration, and apoptosis detection assays to determine the biological role of CD81 in glioblastoma cells.

Results: Our glioblastoma risk score model had extremely high prediction efficacy, with the area under the receiver operating characteristic curve reaching 0.9. The web version of the dynamic nomogram application allows rapid and accurate calculation of patients' survival odds. Survival curves and Sankey diagrams indicated that the high-m6A score group corresponded to the groups expressing medium and low m6A-regulating gene levels and high m6A-associated prognostic immune gene levels. Moreover, these groups displayed lower survival rates and higher immune infiltration. Based on the gene set enrichment analysis, the pathophysiological mechanism may be related to the activation of the immunosuppressive function and related signaling pathways. Moreover, the risk score model allowed us to perform immunotherapy benefit assessment. Finally, silencing CD81 significantly suppressed proliferation, stemness, and migration and facilitated apoptosis in glioblastoma cells.

Conclusion: We developed an accurate and efficient prognostic model. Furthermore, the correlation analysis of different stratification methods with tumor microenvironment provided a basis for further pathophysiological mechanism exploration. Finally, CD81 may serve as a diagnostic and prognostic biomarker in glioblastoma.
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http://dx.doi.org/10.3389/fonc.2022.868415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348864PMC
July 2022

Discovery of Novel 7-Hydroxy-5-oxo-4,5-dihydrothieno[3,2-]pyridine-6-carboxamide Derivatives with Potent and Selective Antifungal Activity against Species.

J Med Chem 2022 Aug 3. Epub 2022 Aug 3.

Department of Pharmacy, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, China.

and can cause fatal invasive infections, especially in immunocompromised patients. However, few antifungal drugs are available to help treat cryptococcosis. In this study, by compound library screening, we presented the first report of hit compound , which had potent and antifungal activity against spp. tests showed that was not cytotoxic and had highly selective and strong antifungal activities against spp. with MIC values less than 1 μg/mL. Synergism of and fluconazole was also observed . The antifungal efficacy of was assessed in a wax moth larval fungal infection model, and treatment with 10 mg/kg caused a significant reduction in fungal burden and significant extension of the survival time. Taken together, our data indicate that the hit compound warrants further investigation as a novel anti- agent.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00794DOI Listing
August 2022

Effects of anterior load carriage on dynamic gait stability during level overground walking.

Hum Mov Sci 2022 Jul 28;85:102981. Epub 2022 Jul 28.

Department of Kinesiology and Health, Georgia State University, 125 Decatur St, Suite-137, Atlanta, GA 30303, USA. Electronic address:

Anterior load carriage, identified as a fall risk factor, is commonly required in daily living activities and occupations. Dynamic gait stability quantifies the kinematic relationship between the human body's center of mass and base of support and has been widely used to assess fall risk. The current study was conducted as a portion of a larger project exploring the effects of anterior load carriage on the control of body stability during various walking conditions. Particularly, this study examined the effect of anterior load carriage on dynamic gait stability during level overground walking among young adults. It was hypothesized that anterior load carriage would compromise dynamic gait stability during walking. Thirty young adults were evenly randomized into three groups: no load (Group 1), 10% body mass (bm) (Group 2), and 20% bm (Group 3). Each group walked overground at a self-selected speed carrying the assigned load. Kinematics were collected for the body and load through motion capture. Dynamic gait stability, gait speed, step length, and trunk angle were determined based on the kinematics and compared between groups. The results did not detect significant load-related effects on dynamic gait stability, step length, or gait speed. A significant load-related difference was found in trunk angle: the heavier the load, the more backward leaned trunk. Further analyses revealed a more posteriorly-leaned trunk in Groups 2 and 3 than Group 1 and in Group 3 than Group 2. The results indicated that young adults could maintain dynamic gait stability when carrying a front load by leaning the trunk backward but keeping other gait parameters unchanged.
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http://dx.doi.org/10.1016/j.humov.2022.102981DOI Listing
July 2022

Prognostic Role of M6A-Associated Immune Genes and Cluster-Related Tumor Microenvironment Analysis: A Multi-Omics Practice in Stomach Adenocarcinoma.

Front Cell Dev Biol 2022 24;10:935135. Epub 2022 Jun 24.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

N6-methylandrostenedione (m6A) methylation plays a very important role in the development of malignant tumors. The immune system is the key point in the progression of tumors, particularly in terms of tumor treatment and drug resistance. Tumor immunotherapy has now become a hot spot and a new approach for tumor treatment. However, as far as the stomach adenocarcinoma (STAD) is concerned, the in-depth research is still a gap in the m6A-associated immune markers. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases is extremely important for our research, where we obtained gene mutation, gene expression data and relevant clinical information of STAD patients. Firstly, the samples from GEO were used as external validation groups, while the TCGA samples were divided into a training group and an internal validation group randomly. Using the way of Single factor COX-LASSO- and multi-factor Cox to construct the prognostic model. Then, all samples were subjected to cluster analysis to generate high and low expression groups of immune gene. Meanwhile, we also collected the correlation between these types and tumor microenvironment. On this basis, a web version of the dynamic nomogram APP was developed. In addition, we performed microenvironmental correlation, copy number variation and mutation analyses for model genes. The prognostic model for STAD developed here demonstrated a very strong predictive ability. The results of cluster analysis manifested that the immune gene low expression group had lower survival rate and higher degree of immune infiltration. Therefore, the immune gene low expression group was associated with lower survival rates and a higher degree of immune infiltration. Gene set enrichment analysis suggested that the potential mechanism might be related to the activation of immunosuppressive functions and multiple signaling pathways. Correspondingly, the web version of the dynamic nomogram APP produced by the DynNom package has successfully achieved rapid and accurate calculation of patient survival rates. Finally, the multi-omics analysis of model genes further enriched the research content. Interference of RAB19 was confirmed to facilitate migration of STAD cells , while its overexpression inhibited these features. The prognostic model for STAD constructed in this study is accurate and efficient based on multi-omics analysis and experimental validation. Additionally, the results of the correlation analysis between the tumor microenvironment and m6Ascore are the basics of further exploration of the pathophysiological mechanism in STAD.
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http://dx.doi.org/10.3389/fcell.2022.935135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291731PMC
June 2022

Reveal the Mechanisms of Yi-Fei-Jian-Pi-Tang on Covid-19 through Network Pharmacology Approach.

Comput Intell Neurosci 2022 16;2022:1493137. Epub 2022 Jul 16.

The Department of Pain Treatment, Gansu Provincial Hospital of TCM, Lanzhou, Gansu 730050, China.

Objectives: The Traditional Chinese Medicine (TCM) formula Yi-Fei-Jian-Pi-Tang (YFJPT) has been demonstrated effective against Corona Virus Disease 2019 (Covid-19). The aim of this article is to make a thorough inquiry about its active constituent as well as mechanisms against Covid-19 via TCM network pharmacology.

Methods: All the ingredients of YFJPT are obtained from the pharmacology database of the TCM system. The genes which are associated with the targets are obtained by utilizing UniProt. The herb-target network is built up by utilizing Cytoscape. The target protein-protein interaction network is built by utilizing the STRING database and Cytoscape. The critical targets of YFJPT are explored by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG).

Results: The outcomes show that YFJPT might has 33 therapeutic targets on Covid-19, namely, interleukin 2 (IL2), heme oxygenase 1 (HMOX1), interleukin 4 (IL4), interferon gamma (FNG), nuclear factor of kappa light polypeptide gene enhancer in Bcells inhibitor, alpha (NFKBIA), nuclear factor-k-gene binding (NFKB), nitric oxide synthase 3 (NOS3), intercellular adhesion molecule 1 (ICAM1), hypoxia inducible factor 1 subunit alpha (HIF1A), mitogen-activated protein kinase 3 (MAPK3), epidermal growth factor receptor (EGFR), interleukin 10 (IL10), jun proto-oncogene (JUN), C-C motif chemokine ligand 2 (CCL2), C-X-C motif chemokine ligand 8 (CXCL8), tumor protein p53 (TP53), interleukin 1 beta (IL1B), AKT serine/threonine kinase 1 (AKT1), tumor necrosis factor (TNF), interleukin 6 (IL6), erb-b2 receptor tyrosine kinase 2 (ERBB2), RELA proto-oncogene (RELA), NF-B subunit, caspase 8 (CASP8), peroxisome proliferator activated receptor alpha (PPARA), TIMP metallopeptidase inhibitor 1 (TIMP1), transforming growth factor beta 1 (TGFB1), interleukin 1 alpha (IL1A), signal transducer and activator of transcription 1 (STAT1), mitogen-activated protein kinase 8 (MAPK8), myeloperoxidase (MPO), matrix metallopeptidase 3 (MMP3), matrix metallopeptidase 1 (MMP1), and NFE2 like bZIP transcription factor 2 (NFE2L2). The gene enrichment analysis prompts that YFJPT most likely contributes to patients related to Covid-19 by regulating the pathways of cancers.

Conclusions: That will lay a foundation for the clinical rational application and further experimental research of YFJPT.
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http://dx.doi.org/10.1155/2022/1493137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288182PMC
July 2022

Effects of Inspiratory Muscle Training and High-Intensity Interval Training on Lung Function and Respiratory Muscle Function in Asthma.

Respir Care 2022 Jul 19. Epub 2022 Jul 19.

Department of Respiratory and Critical Care Medicine, China Rehabilitation Research Center, Rehabilitation School of Capital Medical University, Beijing, China.

Background: Asthma is a heterogeneous disease, usually characterized by chronic airway inflammation. Although inspiratory muscle training (IMT) and high-intensity interval training (HIIT) are beneficial for patients with asthma, controversies persist. Therefore, we aimed to investigate the effects of IMT and HIIT on lung function and respiratory muscle function of subjects with asthma.

Methods: We searched PubMed, Embase, Web of Science, and the Cochrane Library databases up to May 2021. Inclusion criteria were randomized controlled trials (RCTs) of subjects with asthma who received either IMT or HIIT. The outcome measures were changes in lung function and respiratory muscle function.

Results: A total of 13 RCTs (10 in IMT and 3 in HIIT) were included, with a total of 598 subjects. The meta-analysis showed a significantly improved FEV of the expected value (FEV%pred) (mean difference [MD] 4.49% [95% CI 2.31-6.67], < .001; I = 13%), FVC of the expected value (FVC % pred) (MD 5.72% [95% CI 3.56-7.88], < .001; I = 0%), FEV/FVC % (MD 5.01% [95% CI 2.45-7.58], < .001; I = 25%), FVC (L) (MD 0.21 L [95% CI 0.03-0.40], = .02; I = 0%), maximum inspiratory pressure (P) (MD 27.62 cm HO [95% CI 6.50-48.74], = .01; I = 96%), and P (%pred) (MD 27.35% [95% CI 6.94-47.76], = .009; I = 83.5%) in the IMT group. There was no statistical significance in maximum expiratory pressure.

Conclusions: IMT improved pulmonary function (FEV%pred, FVC) and inspiratory muscle strength in subjects with stable asthma. Due to the small number of RCT studies included and the limited outcome measures involving HIIT, we were unable to draw conclusions about whether HIIT was beneficial in this meta-analysis. Moreover, clinical heterogeneity exists in different areas such as population and training programs; the above conclusions still need to be confirmed in future studies.
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http://dx.doi.org/10.4187/respcare.09813DOI Listing
July 2022

Near-infrared fluorescence imaging of thoracic duct in minimally invasive esophagectomy.

Dis Esophagus 2022 Jul 17. Epub 2022 Jul 17.

Department of Thoracic Surgery, Center of Thoracic Mini-invasive Surgery, Peking University People's Hospital, Beijing, China.

Chylothorax is a serious complication after esophagectomy and there are unmet needs for new intraoperative navigation tools to reduce its incidence. The aim of this study is to explore the feasibility and effectiveness of near-infrared fluorescence imaging (NIR-FI) with indocyanine green (ICG) to identify thoracic ducts (TDs) and chyle leakage during video-assisted thoracoscopic esophagectomy. We recruited 41 patients who underwent thoraco-laparoscopic minimally invasive esophagectomy (MIE) for esophageal cancer in this prospective, open-label, single-arm clinical trial. ICG was injected into the right inguinal region before operations, after which TD anatomy and potential chyle leakage were checked under the near-infrared fluorescence intraoperatively. In 38 of 41 patients (92.7%) using NIR-FI, TDs were visible in high contrast. The mean signal-to-background ratio (SBR) value of all fluorescent TDs was 3.05 ± 1.56. Fluorescence imaging of TDs could be detected 0.5 hours after ICG injection and last up to 3 hours with an acceptable SBR value. The optimal observation time window is from about 1 to 2 hours after ICG injection. Under the guidance of real-time NIR-FI, three patients were found to have chylous leakage and the selective TD ligations were performed intraoperatively. No patient had postoperative chylothorax. NIR-FI with ICG can provide highly sensitive and real-time assessment of TDs as well as determine the source of chyle leakage, which might help reduce TD injury and direct selective TD ligation. It could be a promising navigation tool to reduce the incidence of chylothorax after minimally invasive esophagectomy.
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http://dx.doi.org/10.1093/dote/doac049DOI Listing
July 2022

Effects of Exercise on Donkey Semen Parameters and Some Physiological Indices.

J Equine Vet Sci 2022 Jul 16;117:104079. Epub 2022 Jul 16.

National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address:

To study the effects of exercise on donkey semen parameters, Dezhou donkey (Equus asinus) jackasses (n = 18) were assigned to three groups: control (CN, n = 6), 1-hour exercise (1 h EX, n = 6) and 2-hour exercise (2 h EX, n = 6) groups. They were exercised with a walking machine for 3 days/week for 8 weeks. Semen parameters and reproductive hormones were evaluated weekly. The heart rate, rectal temperature, blood haematology indexes, and the body condition score (BCS) were also measured. Results showed that the ejaculation volume increased while the sperm concentration decreased after exercise. However, other sperm parameters were unaffected. Reproductive hormones at resting period were rarely affected by exercise. The red blood cell number increased (P = .023), while the mean erythrocyte hemoglobin concentration decreased (P = .045) after the 8-week exercise in the 2 h EX group compared with the CN. After the 8-week exercise, the resting heart rate of the two exercise groups decreased significantly compared with the CN. The BCS in the CN group increased after the experiment (P = .024). In conclusion, we found that exercise had positive effects on some physiological indices but did not affect semen parameters expect for volume and concentration of the jackass.
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http://dx.doi.org/10.1016/j.jevs.2022.104079DOI Listing
July 2022

Source, occurrence and risks of twenty antibiotics in vegetables and soils from facility agriculture through fixed-point monitoring and numerical simulation.

J Environ Manage 2022 Jul 9;319:115652. Epub 2022 Jul 9.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, PR China. Electronic address:

In this study, a universal method that combined fixed-point monitoring and numerical simulation was used to understand the source, fate and risks of antibiotics in environment. Results showed that the antibiotic concentration in vegetables, soil and manure from 53 fixed-point monitoring sampling sites were ND-18.47, ND-1438.50 and ND-24710.00 μg kg, respectively. There were positive correlations between the antibiotic concentrations of vegetables and soil as well as between soil and manure. The average Amount values were 1.48-46.02, indicating that antibiotics built up pseudo persistent residues in soil due to repeated fertilization. The modified level-III fugacity model showed that tetracyclines and fluoroquinolones tend to remain in soil given their sorption and mobility, while sulfonamides were highly distributed in plants, especially in leaves. Norfloxacin, ofloxacin, sulfadiazine, sulfamethoxazole and sulfisoxazole were found to be risk factors in facility agriculture and should be continuously monitored during agricultural production. Most importantly, we used the inversion method to determine the recommended maximum residue limits of antibiotics in soil. This will not only allow for better control of the amount of the antibiotics in the environment, but also act as a potential method to assess the risks of pollutants without maximum residue limits in the environment.
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http://dx.doi.org/10.1016/j.jenvman.2022.115652DOI Listing
July 2022

Evaluating the impact of universal varicella vaccination among preschool-aged children in Qingdao, China: An interrupted time-series analysis.

Hum Vaccin Immunother 2022 Jul 12:2094641. Epub 2022 Jul 12.

Qingdao Municipal Center for Disease Control and Prevention, Qingdao Institute of Preventive Medicine, Qingdao, China.

Varicella is a contagious disease of children. Qingdao administrated free one-dose and free two-dose universal varicella vaccination schedules in 2013 and 2016 for preschool children. The effectiveness of the vaccination was analyzed in this study. Monthly varicella incidence data of 1-6 years old children during 2007-2020 were obtained from the Qingdao Infectious Disease Reporting Information Management System. We applied Interrupted time series and segmented regression analyses to assess changes in varicella incidence at the beginning of each month and average monthly changes during the vaccination. The vaccination was associated with a reduction of 32.7% in varicella morbidity on average during the 8-year intervention, there is a statistically significant difference between the voluntary period and free vaccination period (χ = 290.80, < 0.001). Immediately after the free one-dose vaccination implementation in 2013 and free two-dose vaccination implementation in 2016, varicella incidence decreased by 0.135 cases per 100 000 population ( < 0.001) and increased by 1.189 cases per 100 000 population ( = 0.039), respectively, the results were statistically significant. There were significant declining trends in varicella incidence after free vaccination: 0.135( < 0.001) and 0.055 ( = 0.025) per month in 2013.7-2016.6 and 2016.7-2020.12, respectively. This study shows a further decaying trend of varicella incidence based on the impact of free two-dose vaccination. It is necessary to prolong free two-dose universal varicella vaccination to strengthen the immune barrier of preschool children sequentially.
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http://dx.doi.org/10.1080/21645515.2022.2094641DOI Listing
July 2022

Carbon aerogels derived from waste paper for pipette-tip solid-phase extraction of triazole fungicides in tomato, apple and pear.

Food Chem 2022 Nov 6;395:133633. Epub 2022 Jul 6.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address:

In order to develop environmentally friendly, economical and facile preparation method of carbon aerogels (CAs), the waste printing paper as the raw material was combined with graphene oxide and carboxylic multi-walled carbon nanotubes to produce CAs (ρ = 44 mg cm). The CAs with different composition were investigated, the addition of graphene oxide led to the reduction of adsorption sites and the reduction of extraction performance. But the carbon nanotubes made CAs have a better pore structure. The CAs as adsorbent were loaded into a pipette-tip for solid-phase extraction of hexaconazole and diniconazole. Coupled with gas chromatography, an analytical method was established under the optimized conditions. The limits of detection were between 0.08 and 0.32 mg kg, the linear ranges were 0.96-200.0 mg kg and 0.24-200.0 mg kg. The relative recoveries were in the range of 81.0-119%. The results indicated that the method had potential application for the determination of triazole fungicides.
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http://dx.doi.org/10.1016/j.foodchem.2022.133633DOI Listing
November 2022

Transcriptional enhancers at 40: evolution of a viral DNA element to nuclear architectural structures.

Trends Genet 2022 Jul 7. Epub 2022 Jul 7.

Howard Hughes Medical Institute, Department and School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address:

Gene regulation by transcriptional enhancers is the dominant mechanism driving cell type- and signal-specific transcriptional diversity in metazoans. However, over four decades since the original discovery, how enhancers operate in the nuclear space remains largely enigmatic. Recent multidisciplinary efforts combining real-time imaging, genome sequencing, and biophysical strategies provide insightful but conflicting models of enhancer-mediated gene control. Here, we review the discovery and progress in enhancer biology, emphasizing the recent findings that acutely activated enhancers assemble regulatory machinery as mesoscale architectural structures with distinct physical properties. These findings help formulate novel models that explain several mysterious features of the assembly of transcriptional enhancers and the mechanisms of spatial control of gene expression.
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http://dx.doi.org/10.1016/j.tig.2022.05.015DOI Listing
July 2022

Gene Family: Identification and Expression Profiles during Leaf Vein Development in Soybean.

Plants (Basel) 2022 Jun 29;11(13). Epub 2022 Jun 29.

College of Agronomy, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu 611130, China.

Leaf veins constitute the transport network for water and photosynthetic assimilates in vascular plants. The class III homeodomain-leucine zipper () gene family is central to the regulation of vascular development. In this research, we performed an overall analysis of the genes in soybean ( L. Merr.). Our analysis included the phylogeny, conservation domains and -elements in the promoters of these genes. We used the quantitative reverse transcription-polymerase chain reaction to characterize the expression patterns of genes in leaf vein development and analyze the effects of exogenous hormone treatments. In this study, twelve genes were identified from the soybean genome and named. All soybean HD-Zip III proteins contained four highly conserved domains. transcripts showed steadily increasing accumulation during all stages of leaf vein development and were highly expressed in cambium cells. and had nearly identical expression patterns in soybean leaf vein tissues. and transcripts remained at stable high levels at all xylem developmental stages. and were expressed at high levels in the vascular cambium and xylem cells. Overall, may be an essential regulator that is responsible for the formation or maintenance of soybean vein cambial cells. and were correlated with xylem differentiation in soybean leaf veins. This study will pave the way for identifying the molecular mechanism of leaf vein development.
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http://dx.doi.org/10.3390/plants11131728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269512PMC
June 2022

Pixel Super-Resolution Phase Retrieval for Lensless On-Chip Microscopy via Accelerated Wirtinger Flow.

Cells 2022 Jun 22;11(13). Epub 2022 Jun 22.

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing 100084, China.

Empowered by pixel super-resolution (PSR) and phase retrieval techniques, lensless on-chip microscopy opens up new possibilities for high-throughput biomedical imaging. However, the current PSR phase retrieval approaches are time consuming in terms of both the measurement and reconstruction procedures. In this work, we present a novel computational framework for PSR phase retrieval to address these concerns. Specifically, a sparsity-promoting regularizer is introduced to enhance the well posedness of the nonconvex problem under limited measurements, and Nesterov's momentum is used to accelerate the iterations. The resulting algorithm, termed accelerated Wirtinger flow (AWF), achieves at least an order of magnitude faster rate of convergence and allows a twofold reduction in the measurement number while maintaining competitive reconstruction quality. Furthermore, we provide general guidance for step size selection based on theoretical analyses, facilitating simple implementation without the need for complicated parameter tuning. The proposed AWF algorithm is compatible with most of the existing lensless on-chip microscopes and could help achieve label-free rapid whole slide imaging of dynamic biological activities at subpixel resolution.
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http://dx.doi.org/10.3390/cells11131999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265759PMC
June 2022

Potential value of PRKDC as a therapeutic target and prognostic biomarker in pan-cancer.

Medicine (Baltimore) 2022 Jul 8;101(27):e29628. Epub 2022 Jul 8.

Transplant Medical Center, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.

Background: While protein kinase, DNA-activated, catalytic subunit (PRKDC) plays an important role in double-strand break repair to retain genomic stability, there is still no pan-cancer analysis based on large clinical information on the relationship between PRKDC and different tumors. For the first time, this research used numerous databases to perform a pan-cancer review for PRKDC to explore the possible mechanism of PRKDC in the etiology and outcomes in various tumors.

Methods: PRKDC's expression profile and prognostic significance in pan-cancer were investigated based on various databases and online platforms, including TIMER2, GEPIA2, cBioPortal, CPTAC, and SangerBox. We applied the TIMER to identified the interlink of PRKDC and the immune infiltration in assorted tumors, and the SangerBox online platform was adopted to find out the relevance between PRKDC and immune checkpoint genes, tumor mutation burden, and microsatellite instability in tumors. GeneMANIA tool was employed to create a protein-protein interaction analysis, gene set enrichment analysis was conducted to performed gene enrichment analysis.

Results: Overall, tumor tissue presented a higher degree of PRKDC expression than adjacent normal tissue. Meanwhile, patients with high PRKDC expression have a worse prognosis. PRKDC mutations were present in almost all The Cancer Genome Atlas tumors and might lead to a better survival prognosis. The PRKDC expression level was shown a positive correlation with tumor-infiltrating immune cells. PRKDC high expression cohorts were enriched in "cell cycle" "oocyte meiosis" and "RNA-degradation" signaling pathways.

Conclusions: This study revealed the potential value of PRKDC in tumor immunology and as a therapeutic target and prognostic biomarker in pan-cancer.
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http://dx.doi.org/10.1097/MD.0000000000029628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259106PMC
July 2022

Evaluation of anti-tick efficiency in rabbits induced by DNA vaccines encoding Haemaphysalis longicornis lipocalin homologue.

Med Vet Entomol 2022 Jul 8. Epub 2022 Jul 8.

Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Province Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, PR China.

Haemaphysalis longicornis is an obligate haematophagous ectoparasite, transmitting a variety of pathogens, which brings great damage to human health and animal husbandry development. Lipocalins (LIP) are a family of proteins that transport small hydrophobic molecules and also involve in immune regulation, such as the regulation of cell homeostasis, inhibiting the host's inflammatory response and resisting the contractile responses in host blood vessels. Therefore, it is one of the candidate antigens for vaccines. Based on previous studies, we constructed the recombinant plasmid pcDNA3.1-HlLIP of LIP homologue from H. longicornis (HlLIP). ELISA results showed that rabbits immunized with pcDNA3.1-HlLIP produced higher anti-rHlLIP antibody levels compared with the pcDNA3.1 group, indicating that pcDNA3.1-HlLIP induced the humoral immune response of host. Adult H. longicornis infestation trial in rabbits demonstrated that the engorgement weight, oviposition and hatchability of H. longicornis fed on rabbits immunized with pcDNA3.1-HlLIP decreased by 7.07%, 14.30% and 11.70% respectively, compared with that of the pcDNA3.1 group. In brief, DNA vaccine of pcDNA3.1-HlLIP provided immune protection efficiency of 30% in rabbits. This study demonstrated that pcDNA3.1-HlLIP can partially protect rabbits against H. longicornis, and it is possible to develop a new candidate antigen against ticks.
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http://dx.doi.org/10.1111/mve.12594DOI Listing
July 2022

Epitheliocystis caused by a novel Chlamydia emerging in Spinibarbus denticulatus in China.

Dis Aquat Organ 2022 Jul 7;150:31-36. Epub 2022 Jul 7.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.

Epitheliocystis is an emerging and global aquaculture disease caused by a diverse range of bacteria of the order Chlamydiales. Here we report a case of epitheliocystis caused by a novel Chlamydia bacterium, which resulted in 40% mortality in cultured cyprinids (Spinibarbus denticulatus). The affected fish exhibited lethargy, were observed swimming near the oxygen pump and subsequently died. Histopathology analysis revealed that lesions were concentrated mainly on the gills. The epithelial cells of the damaged gill lamellae showed hyperplasia, fusion and adhesion, and were characterized by inflammation and necrosis. Inclusion bodies were observed in some proliferating epithelial cells at the tips of the gill lamellae and were accompanied by different degrees of mucous cell proliferation. Transmission electron microscopy examination clearly showed the morphological characteristics of chlamydia-like bacteria in epithelial cells. In addition, 16S rRNA sequencing (752 bp) and molecular phylogenetic analyses revealed that epitheliocystis agents detected in S. denticulatus belonged to a novel family, Chlamydiaceae. This is the first report of epitheliocystis in cultured fish in China, and the findings in this study increase the range of hosts affected by epitheliocystis.
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http://dx.doi.org/10.3354/dao03672DOI Listing
July 2022

Comparison of left and right-sided colon cancer: A study based on clinical pathology and serum amino acid metabolism.

Asian J Surg 2022 Jul 3. Epub 2022 Jul 3.

The Second Ward of Colorectal Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, Liaoning, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.05.067DOI Listing
July 2022

Unified, Asymmetric Total Synthesis of the Asnovolins and Related Spiromeroterpenoids: A Fragment Coupling Approach.

J Am Chem Soc 2022 Jul 6;144(28):12970-12978. Epub 2022 Jul 6.

Department of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United States.

3,5-Dimethylorsellinic acid (DMOA)-derived spiromeroterpenoids are a unique natural product family with attractive structures, unconventional stereochemistry, and potent biological activities. Herein, we report the first asymmetric total syntheses of the asnovolins, DMOA-derived spiromeroterpenoids. The spirocyclic skeleton was efficiently assembled through a sterically hindered bis-neopentyl 1,2-addition coupling/oxidative Michael addition sequence. The unusual axial C12-methyl stereochemistry was established metal hydrogen atom transfer (MHAT) reduction involving a chair-to-boat conformational change. The mechanism of the HAT process was studied through both deuterium labeling and computational studies. Attempted late-stage alkene isomerization of an exocyclic enone proved to be challenging and resulted in hetero-Diels-Alder dimerization, which ultimately led to development of an alternative desaturation/coupling sequence. Endgame core modifications including orthogonal desaturation, Sc(III)-promoted regioselective Baeyer-Villiger oxidation, and Meerwein-Ponndorf-Verley reduction enabled collective syntheses of five asnovolin-related natural products. This study demonstrates the utility of anionic fragment coupling to assemble a sterically congested molecular framework and provides a foundation for the synthesis of spiromeroterpenoid congeners with higher oxidation states for biological studies.
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http://dx.doi.org/10.1021/jacs.2c05366DOI Listing
July 2022

Recurrent Pulmonary Embolism Associated with a New Mutation Site of SLC44A2: A Case Report.

Anatol J Cardiol 2022 07;26(7):577-579

Department of Respiratory Medicine, the First Affiliated Hospital of Guangxi Medical University, Guangxi, China.

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http://dx.doi.org/10.5152/AnatolJCardiol.2022.1585DOI Listing
July 2022

Corticosterone antagonist or TrkB agonist attenuates schizophrenia-like behavior in a mouse model combining deficiency and developmental stress.

iScience 2022 Jul 16;25(7):104609. Epub 2022 Jun 16.

School of Pharmaceutical Sciences, IDG/McGovern Institute for Brain Research, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing 100084, China.

While schizophrenia pathogenesis involves both genetic and environmental factors, their specific combinations remain ill-defined. Here we show that deficiency in promoter VI-driven BDNF expression, combined with early-life adversity, results in schizophrenia-like endo-phenotypes. Promoter VI mutant mice (e6 ), when exposed to postnatal stress including hypoxia or social isolation, exhibited deficits in social interactions, spatial memory, and sensorimotor gating reflected by prepulse inhibition (PPI). Neither early-life stress nor e6 deficiency alone caused these abnormalities. Moreover, postnatal stress increased blood corticosterone levels of wild-type mice, and administration of corticosterone to -e6 mice without early-life stress also resulted in PPI deficits and social dysfunction. Finally, the PPI deficits in postnatally stressed -e6 mice were rescued by treatment with the corticosterone antagonist RU-486, or the BDNF mimetic TrkB agonistic antibody. Thus, we have identified a pair of genetic and environmental factors contributing to schizophrenia pathogenesis and providing a potential strategy for therapeutic interventions for schizophrenia.
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http://dx.doi.org/10.1016/j.isci.2022.104609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250029PMC
July 2022

Developing a Copper(II) Agent Based on His-146 and His-242 Residues of Human Serum Albumin Nanoparticles: Integration To Overcome Cisplatin Resistance and Inhibit the Metastasis of Nonsmall Cell Lung Cancer.

J Med Chem 2022 07 4;65(13):9447-9458. Epub 2022 Jul 4.

School of Chemistry and Pharmaceutical Sciences, State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, Guangxi Normal University, Guilin, Guangxi 541004, China.

To overcome the resistance of nonsmall cell lung cancer (NSCLC) cells to cisplatin and inhibit their metastasis, we proposed to develop a Cu(II) agent based on the specific residue(s) of HSA nanoparticles (NPs) for multitargeting the tumor microenvironment (TME). To this end, we not only synthesized four Cu(II) 2-hydroxy-3-methoxybenzaldehyde thiosemicarbazone compounds (C1-C4), obtaining a Cu compound (C4) with significant cytotoxicity by studying their structure-activity relationships, but also revealed the binding mechanism of C4 to HSA through X-ray crystallography and confirmed the successful construction of a new HSA-C4 NPs delivery system. C4 and HSA-C4 NPs inhibited the A549cisR tumor growth and metastasis, and HSA NPs optimized the anticancer behavior of C4. We further confirmed the anticancer mechanism of the C4/HSA-C4 NP multitargeting TME to overcome cisplatin resistance: killing tumor cells by acting on the mtDNA and inducing apoptosis, polarizing M2-type macrophages to the M1-type, and inhibiting angiogenesis.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00698DOI Listing
July 2022

Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library.

Biotechnol Lett 2022 Aug 30;44(8):951-960. Epub 2022 Jun 30.

College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.

Objectives: Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.

Results: After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.

Conclusions: Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer.
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http://dx.doi.org/10.1007/s10529-022-03263-wDOI Listing
August 2022

Coupling Chlorin-Based Photosensitizers and Histone Deacetylase Inhibitors for Photodynamic Chemotherapy.

Mol Pharm 2022 08 27;19(8):2807-2817. Epub 2022 Jun 27.

Department of Pharmacy, Hebei Key Laboratory of Neuropharmacology, Hebei North University, Zhangjiakou 075000, P.R. China.

Photodynamic therapy combined with chemotherapy is a promising strategy to improve the antitumor efficacy. On the basis of coupling the chlorin-based photosensitizer pyropheophorbide a (Pyro) and histone deacetylase inhibitors (HDACis) to fabricate dual-mode antitumor molecules, a series of dual-mode antitumor prodrug molecules were synthesized and assessed for antitumor activity in vitro and in vivo. The data demonstrated that , with the most favorable phototoxicity and dark toxicity, could significantly inhibit the cell migration and upregulate the expression of acetyl-H3 protein, functioning as a photosensitizer and HDACi, respectively. Furthermore, compared with talaporfin, Pyro, and SAHA, demonstrated the best inhibitory effect on tumor growth and metastasis in tumor-bearing mice; therefore, represented by , this pharmacophore coupling strategy is much more promising and effective than the pharmacophore fusion strategy for fabricating photodynamic and chemotherapeutical dual-mode molecules.
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http://dx.doi.org/10.1021/acs.molpharmaceut.2c00170DOI Listing
August 2022

Regional analysis of the characteristics and potential risks of bacterial pathogen resistance under high-pressure antibiotic application.

J Environ Manage 2022 Sep 8;317:115481. Epub 2022 Jun 8.

College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China.

The study aimed to perform a regional investigation of the antibiotic resistance characteristics (ARCHs) of zoonotic pathogens in environments of high antibiotic pressure to observe the future trend of antibiotic application. In this study, an ARCH analysis of the animal pathogens was conducted in the Sichuan Basin with an area of about 180,000 km and an estimated high antibiotic application exceeding 2000 tons. A total of 388 bacterial strains from nine species were isolated during 2013-2021. The results showed a dynamic change in the pathogen resistance in the Sichuan Basin with no apparent temporal trend. Fifty-two of 54 antibiotic resistance phenotypes (ARPs) and 180/218 antibiotic resistance genes (ARGs) were detected in this region. The antibiotic resistance in the classification of β-lactam, sulfanilamide, and tetracycline had a relatively high detective rate, with 33-58% of ARPs and about 29.7% of ARGs. The isolates from terrestrial animals generally had higher ARPs and ARGs than aquatic animals. Most pathogens carried 5-11 ARPs, and each isolate carried 19.7 ARGs on average. Our result showed that there was a complicated accumulation of ARGs under high antibiotic pressure. Besides, the unique strain in the Sichuan Basin did not show higher resistance rates compared with the World Health Organization data, possibly due to fitness cost. However, the complex ARCH under high pressure still deserves attention to prevent the emergence of super-resistant bacteria.
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http://dx.doi.org/10.1016/j.jenvman.2022.115481DOI Listing
September 2022

Value of prediction model in distinguishing gallbladder adenoma from cholesterol polyp.

J Gastroenterol Hepatol 2022 Jun 24. Epub 2022 Jun 24.

Department of Hepatobiliary Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University (Navy Medical University), Shanghai, China.

Background And Aim: Gallbladder adenomatous polyp is a pre-cancerous neoplasm, and it is difficult to classify from cholesterol polyps before cholecystectomy. The study aimed to clarify the risk characteristics of gallbladder adenomas and establish a prediction model to differentiate gallbladder adenomas from cholesterol polyp lesions.

Methods: From May 2019 to December 2021, the patients underwent cholecystectomy in the Shanghai Eastern Hepatobiliary Surgery Hospital were retrospectively reviewed. According to the permanent pathology test, the patients were divided into adenomas and cholesterol polyps groups. All the included cases received ultrasound equipment examinations before cholecystectomy and their clinical information were completely recorded. Then the patients' baseline characteristics and ultrasound imaging variables were analyzed by logistic regression. Finally, a predictive model for gallbladder adenomas will be established and assessed based on the independent risk factors.

Results: A total of 423 cases including 296 cholesterol polyps and 127 gallbladder adenomas were analyzed in detail. Multivariate logistic regression analysis revealed that solitary polyp lesion (OR = 2.954, 95% CI 1.759-4.960, P < 0.001), the maximal diameter of lesions (OR = 1.244, 95% CI 1.169-1.324, P < 0.001), and irregular shape of polyp lesions (OR = 5.549, 95% CI 1.979-15.560, P = 0.001) were the independent predictive factors of gallbladder adenomas. According to the results, regression equation of logit(P) = -3.828 + 1.083*number of gallbladder polyps lesions (GPLs) + 0.218*diameter of GPLs + 1.714*shape of GPLs was established. Area under the curve (AUC) was 0.828 (95% CI 0.782-0.874, P < 0.001). When logit P > 0.204, the sensitivity of estimating adenoma was 79.5%, the specificity of recognizing adenoma was 70.6%, and the whole correct ratio was 73.3%. While the AUC of diameter (10 mm) being a predictive factor in this study was only 0.790 (95% CI 0.741-0.839, P < 0.001). And the sensitivity and specificity of 10 mm as the optimal diagnostic cutoff value to diagnose adenomas were 74.8% and 65.9%, respectively.

Conclusions: The risk factors of solitary polyp lesion, larger diameter, and irregular morphology feature of polyp lesions were significantly related to gallbladder adenomas. And the predictive model established in the study can effectively identify adenomas from cholesterol polyps and help patients to select the optimal treatment protocol.
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http://dx.doi.org/10.1111/jgh.15928DOI Listing
June 2022

Nitrogen-rich ion salts of 1-hydroxytetrazole-5-hydrazide: a new series of energetic compounds that combine good stability and high energy performance.

Dalton Trans 2022 Jul 5;51(26):10216-10220. Epub 2022 Jul 5.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China.

High-efficiency explosives that combine high stability and excellent energy performance are one of the key directions of energetic materials research. In this study, a novel monocyclic hydroxytetrazole derivative (3) with high stability was prepared, and a series of insensitive energetic ionic salts were derived from it. Benefiting from their outstanding performance in terms of density, 3D hydrogen bonding and π-electron interactions, these salts are excellent in both detonation performance ( = 8709 to 9314 m s and = 29.9 to 35.6 GPa) and thermal stability ( = 193.0-232.2 °C). The hydrazine salt (2) exhibits high detonation properties ( = 9314 m s and = 35.6 GPa), due to its high density ( = 1.71 g cm) and high heat of formation (Δ = 563.2 kJ mol = 3.19 kJ g). In addition, the high thermal stability ( = 232.0 °C) and low mechanical sensitivity (IS = 30 J and FS = 360 N) of 2 are also unmatched by HMX and TKX-50. These improved properties demonstrate the great promise of 2 as an insensitive high-energy explosive.
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http://dx.doi.org/10.1039/d2dt01173eDOI Listing
July 2022
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