Publications by authors named "Feng Qin"

540 Publications

Effect of Root Canal Therapy Combined with Full Crown Restoration on the Level of Inflammatory Factors and Chewing Function in Patients with Cracked Teeth and Chronic Pulpitis.

Evid Based Complement Alternat Med 2021 30;2021:3299349. Epub 2021 Aug 30.

Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Huangpu, Shanghai 200011, China.

Objective: To explore the clinical effect of root canal therapy combined with full crown restoration in patients with cracked teeth and chronic pulpitis.

Methods: From May 2018 to June 2020, 87 patients with cracked teeth and chronic pulpitis in our hospital were selected; the patients were randomly divided into the control group and the research group by random number method. The control group only used root canal therapy; the research group used root canal therapy combined with full crown restoration. The therapeutic effect, levels of inflammatory factors, chewing function, periodontal index, complications, and quality of life were compared between the two groups.

Results: The total effective rate of the research group (97.78%) was better than the total effective rate of the control group (85.71%) ( < 0.05). Compared with before treatment, the serum levels of interleukin-1 (IL-1), IL-6, and C-reactive protein (CRP) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the serum levels of IL-1, IL-6, and CRP in the research group decreased ( < 0.05). Compared with before treatment, the bite force of teeth and chewing efficiency of the two groups of patients increased after treatment. After treatment, compared with the control group, the bite force of teeth and chewing efficiency of the research group increased ( < 0.05). Compared with before treatment, the plaque index (PLI), probing depth (PD), gingival sulcus bleeding index (BI), and gingival index (GI) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the PLI, PD, BI, and GI of the research group decreased ( < 0.05). The total incidence of complications in the research group was (11.11%), and the total incidence of complications in the control group was (16.67%); there was no significant difference between the two groups ( > 0.05). After treatment, compared with the control group, the quality of life scores of the patients in the research group were reduced ( < 0.05).

Conclusion: Root canal therapy and full crown restoration have a definite curative effect in patients with cracked teeth and chronic pulpitis, which can improve the inflammatory response, restore chewing function, maintain periodontal health, improve the quality of life, and do not increase the incidence of complications, so it has good application value.
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http://dx.doi.org/10.1155/2021/3299349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423566PMC
August 2021

Three-dimensional monolithic micro-LED display driven by atomically thin transistor matrix.

Nat Nanotechnol 2021 Sep 9. Epub 2021 Sep 9.

National Laboratory of Solid State Microstructures, School of Electronic Science and Engineering and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, China.

Two-dimensional materials are promising candidates for future electronics due to unmatched device performance at atomic limit and low-temperature heterogeneous integration. To adopt these emerging materials in computing and optoelectronic systems, back end of line (BEOL) integration with mainstream technologies is needed. Here, we show the integration of large-area MoS thin-film transistors (TFTs) with nitride micro light-emitting diodes (LEDs) through a BEOL process and demonstrate high-resolution displays. The MoS transistors exhibit median mobility of 54 cm Vs , 210 μA μm drive current and excellent uniformity. The TFTs can drive micrometre-sized LEDs to 7.1 × 10 cd m luminance under low voltage. Comprehensive analysis on driving capability, response time, power consumption and modulation scheme indicates that MoS TFTs are suitable for a range of display applications up to the high resolution and brightness limit. We further demonstrate prototypical 32 × 32 active-matrix displays at 1,270 pixels-per-inch resolution. Moreover, our process is fully monolithic, low-temperature, scalable and compatible with microelectronic processing.
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http://dx.doi.org/10.1038/s41565-021-00966-5DOI Listing
September 2021

Genomic basis underlying the metabolome-mediated drought adaptation of maize.

Genome Biol 2021 09 6;22(1):260. Epub 2021 Sep 6.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Drought is a major environmental disaster that causes crop yield loss worldwide. Metabolites are involved in various environmental stress responses of plants. However, the genetic control of metabolomes underlying crop environmental stress adaptation remains elusive.

Results: Here, we perform non-targeted metabolic profiling of leaves for 385 maize natural inbred lines grown under well-watered as well as drought-stressed conditions. A total of 3890 metabolites are identified and 1035 of these are differentially produced between well-watered and drought-stressed conditions, representing effective indicators of maize drought response and tolerance. Genetic dissections reveal the associations between these metabolites and thousands of single-nucleotide polymorphisms (SNPs), which represented 3415 metabolite quantitative trait loci (mQTLs) and 2589 candidate genes. 78.6% of mQTLs (2684/3415) are novel drought-responsive QTLs. The regulatory variants that control the expression of the candidate genes are revealed by expression QTL (eQTL) analysis of the transcriptomes of leaves from 197 maize natural inbred lines. Integrated metabolic and transcriptomic assays identify dozens of environment-specific hub genes and their gene-metabolite regulatory networks. Comprehensive genetic and molecular studies reveal the roles and mechanisms of two hub genes, Bx12 and ZmGLK44, in regulating maize metabolite biosynthesis and drought tolerance.

Conclusion: Our studies reveal the first population-level metabolomes in crop drought response and uncover the natural variations and genetic control of these metabolomes underlying crop drought adaptation, demonstrating that multi-omics is a powerful strategy to dissect the genetic mechanisms of crop complex traits.
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http://dx.doi.org/10.1186/s13059-021-02481-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420056PMC
September 2021

Ultra-wideband and wide-angle perfect solar energy absorber based on Ti nanorings surface plasmon resonance.

Phys Chem Chem Phys 2021 Aug;23(31):17041-17048

School of Science, East China Jiaotong University, Nanchang 330013, China.

Solar energy absorption is a very important field in photonics. The successful development of an efficient, wide-band solar absorber is an extremely powerful driver in this field. We propose an ultra-wideband (UWB) solar energy absorber composed of a Ti ring and SiO2-Si3N4-Ti thin films. In the range of 300-4000 nm, the wide band has an absorption efficiency of more than 90% and can reach 3683 nm, and it has four absorption peaks with a high absorptivity. Moreover, the weighted average absorption efficiency of the solar absorber under AM 1.5 is maintained above 97.03%, which indicates it has great potential for use in the field of solar energy absorption. Moreover, we proved that the polarization is insensitive by analyzing the absorption characteristics at arbitrary polarization angles. For both the transverse electric (TE) and transverse magnetic (TM) modes, the UWB absorption is maintained at more than 90% in the wide incidence angle range of 60°. The UWB solar energy absorber has great potential for use in a variety of applications, such as converting solar light and heat into electricity for public use and reducing the side effects of coal-fired power generation. It can also be used in information detection and infrared thermal imaging owing to its UWB characteristics.
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http://dx.doi.org/10.1039/d1cp03036aDOI Listing
August 2021

Prolonged isoflurane anesthesia-induced acidosis decreases penile intracavernous pressure in rats.

Andrology 2021 Aug 1. Epub 2021 Aug 1.

Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.

Background: Intracavernous pressure measurement following cavernous nerve electrostimulation has been extensively adopted for the evaluation of erectile function in animals. However, the effect of measurement time and acidosis during anesthesia is still lacking.

Objective: To explore the effect of measurement time and acidosis during anesthesia.

Materials And Methods: Fifty-six male Sprague-Dawley rats were used and anesthetized by a spontaneous inhalation of isoflurane. In the first step, rats were randomly divided into four groups: a control group and three time-delayed measurement groups (intracavernous pressure measurement beginning at 15, 30, and 45 min after cavernous nerve exposure). In the second step, rats were randomly divided into three groups: a control group and two time-delayed measurement groups. Two intravenous fluid support strategies were used in time-delayed measurement groups: a normal saline solution and an isotonic Na CO solution.

Results: Isoflurane-anesthetized rats developed systemic acidosis that worsens with time during intracavernous pressure measurement, which results in a significant decrease in the maximum intracavernous pressure value, intracavernous pressure/mean arterial pressure ratio, and total intracavernous pressure measured. The Na CO infusion could effectively correct acidosis. The decrease in intracavernous pressure was related to the reduced nitric oxide synthase activity, decreased cyclic guanosine monophosphate concentration, and reactive oxygen species activation in rat penis under acidosis conditions.

Discussion And Conclusion: Prolonged isoflurane anesthesia-induced acidosis markedly depresses the erectile response to cavernous nerve electrostimulation in rats. In this situation, it is recommended to supplement with a Na CO infusion to maintain a normal acid-base balance.
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http://dx.doi.org/10.1111/andr.13085DOI Listing
August 2021

Stereotactic biopsy for lesions in brainstem and deep brain: a single-center experience of 72 cases.

Braz J Med Biol Res 2021 23;54(8):e11335. Epub 2021 Jul 23.

Department of Neurology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Stereotactic biopsies for lesions in the brainstem and deep brain are rare. This study aimed to summarize our 6-year experience in the accurate diagnosis of lesions in the brain stem and deep brain and to discuss the technical note and strategies. From December 2011 to January 2018, 72 cases of intracranial lesions in the brainstem or deep in the lobes undergoing stereotactic biopsy were retrospectively reviewed. An individualized puncture path was designed based on the lesion's location and the image characteristics. The most common biopsy targets were deep in the lobes (43 cases, 59.7%), including frontal lobe (33 cases, 45.8%), temporal lobe (4 cases, 5.6%), parietal lobe (3 cases, 4.2%), and occipital lobe (3 cases, 4.2 %). There were 12 cases (16.7%) of the brainstem, including 8 cases (11.1%) of midbrain, and 4 cases (5.6%) of pons or brachium pontis. Other targets included internal capsule (2 cases, 2.8%), thalamus (3 cases, 4.2%), and basal ganglion (12 cases, 16.7%). As for complications, one patient developed acute intracerebral hemorrhage in the biopsy area at 2 h post-operation, and one patient had delayed intracerebral hemorrhage at 7 days post-operation. The remaining patients recovered well after surgery. There was no surgery-related death. The CT-MRI-guided stereotactic biopsy of lesions in the brainstem or deep in the brain has the advantages of high safety, accurate diagnosis, and low incidence of complications. It plays a crucial role in the diagnosis of atypical, microscopic, diffuse, multiple, and refractory lesions.
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http://dx.doi.org/10.1590/1414-431X2021e11335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302144PMC
July 2021

Preparation parameters of polyaniline/polyvinyl chloride flexible wires for electrical conductivity performance analysis based on orthogonal arrays.

Des Monomers Polym 2021 28;24(1):191-198. Epub 2021 Jun 28.

Testing Center, Yangzhou University, Yangzhou, PR China.

A flexible polyaniline/polyvinyl chloride (PVC) polymer conductive wire was prepared using flexible PVC polymer as the substrate by the swelling - in-situ polymerization method, the line-shaped dents were pressed on the substrate by the thermodynamic pre-deformation treatment technology. Based on the orthogonal test method, the effects of five main influencing factors - swelling time (A), swelling temperature (B), oxidation temperature (C), oxidation time (D), and oxidant concentration (E) - on the conductivity of the prepared polyaniline/PVC conductive wire was investigated. The results of the orthogonal array testing were subjected to range analysis and analysis of variance (ANOVA), and the influencing factors, in terms of significance, follow the order of swelling temperature, oxidation time, swelling time, oxidation temperature, and oxidant concentration, with the optimal factor-level combination being ABCDE, which led to a desirable conductivity up to 1.19 × 10 S/cm. In addition, the influence of different conductive line size characteristics on the molecular structure, microstructure, and conductivity of polyaniline/PVC flexible conductive wire was further studied. On the microstructure, as the line width increases, the infrared absorption intensity ratio of the quinone ring and the benzene ring in the polyaniline/PVC conductive wires gradually approaches 1. The microstructure, as the line width of the polyaniline/PVC conductive wire increases, the formed polyaniline gradually changes from flakes and granules to fibrous strips and entangles with each other to form a spatial network structure. The conductivity of the wire increases with the increase of its width up to 1.48 × 10 S/cm.
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http://dx.doi.org/10.1080/15685551.2021.1936373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245068PMC
June 2021

The neurotrophic and antineuroinflammatory effects of phenylpropanoids from Zanthoxylum nitidum var. tomentosum (Rutaceae).

Fitoterapia 2021 Sep 9;153:104990. Epub 2021 Jul 9.

State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, People's Republic of China. Electronic address:

Three novel lignans (1, 5 and 6) and two novel quinic acids (16 and 17) along with 15 known phenylpropanoids were obtained from the ethanol extract of Zanthoxylum nitidum var. tomentosum (Rutaceae). Their structures were confirmed by comprehensive spectroscopic data (NMR and HRESIMS), and the absolute configurations of all novel compounds were elucidated based on electronic circular dichroism (ECD) spectroscopic data. The production of nitric oxide (NO) in BV-2 microglial cells induced through lipopolysaccharide (LPS) was used to evaluate in vitro anti-neuroinflammatory activity of compounds 1-20. Compound 2, 3, 7 and 16 showed excellent inhibition of LPS-induced NO production. The structure-activity relationships of the isolates were investigated. In addition, the mechanism of action of 2 was elucidated by RT-PCR and Western blotting analysis, which indicated that it reduced neuroinflammatory mainly through NLRP3/caspase1 signaling pathways in LPS-induced BV2 microglial cells.
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http://dx.doi.org/10.1016/j.fitote.2021.104990DOI Listing
September 2021

Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance.

Genome Biol 2021 06 24;22(1):185. Epub 2021 Jun 24.

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, and Hubei Key Laboratory of Agricultural Bioinformatics, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Drought threatens the food supply of the world population. Dissecting the dynamic responses of plants to drought will be beneficial for breeding drought-tolerant crops, as the genetic controls of these responses remain largely unknown.

Results: Here we develop a high-throughput multiple optical phenotyping system to noninvasively phenotype 368 maize genotypes with or without drought stress over a course of 98 days, and collected multiple optical images, including color camera scanning, hyperspectral imaging, and X-ray computed tomography images. We develop high-throughput analysis pipelines to extract image-based traits (i-traits). Of these i-traits, 10,080 were effective and heritable indicators of maize external and internal drought responses. An i-trait-based genome-wide association study reveals 4322 significant locus-trait associations, representing 1529 quantitative trait loci (QTLs) and 2318 candidate genes, many that co-localize with previously reported maize drought responsive QTLs. Expression QTL (eQTL) analysis uncovers many local and distant regulatory variants that control the expression of the candidate genes. We use genetic mutation analysis to validate two new genes, ZmcPGM2 and ZmFAB1A, which regulate i-traits and drought tolerance. Moreover, the value of the candidate genes as drought-tolerant genetic markers is revealed by genome selection analysis, and 15 i-traits are identified as potential markers for maize drought tolerance breeding.

Conclusion: Our study demonstrates that combining high-throughput multiple optical phenotyping and GWAS is a novel and effective approach to dissect the genetic architecture of complex traits and clone drought-tolerance associated genes.
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http://dx.doi.org/10.1186/s13059-021-02377-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223302PMC
June 2021

Pelvic lipomatosis with cystitis glandularis managed with cyclooxygenase-2 inhibitor: A case report.

World J Clin Cases 2021 Jun;9(17):4373-4380

Department of Urology, Taizhou Hospital of Zhejiang Province Affiliated with Wenzhou Medical University, Taizhou 317000, Zhejiang Province, China.

Background: Pelvic lipomatosis (PL) is a rare benign condition with characteristic overgrowth of histologically benign fat and invasion and compression of pelvic organs, often leading to non-specific lower urinary tract symptoms (LUTS). Approximately 40% of patients with PL have cystitis glandularis (CG). The cause of PL combined with CG is poorly understood, and there is currently no effective treatment. Refractory CG with upper urinary tract obstruction even requires partial or radical bladder resection.

Case Summary: In this case, a patient suffering from PL with CG was treated by transurethral resection of bladder tumour (TUR-BT) and oral administration of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor. The LUTS were alleviated, and the cystoscopy results improved significantly. Immunohistochemistry showed up-regulated COX-2 expression in the epithelium of TUR-BT samples, suggesting that COX-2 may participate in the pathophysiological process of PL combined with CG.

Conclusion: We report for the first time that celecoxib may be an effective treatment strategy for PL combined with refractory CG.
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http://dx.doi.org/10.12998/wjcc.v9.i17.4373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173416PMC
June 2021

Probing the Chiral Domains and Excitonic States in Individual WS Tubes by Second-Harmonic Generation.

Nano Lett 2021 Jun 11;21(12):4937-4943. Epub 2021 Jun 11.

State Key Laboratory of Surface Physics, Key Laboratory of Micro and Nano Photonic Structures (MOE), and Department of Physics, Fudan University, Shanghai 200433, People's Republic of China.

Distinct from carbon nanotubes, transition-metal dichalcogenide (TMD) nanotubes are noncentrosymmetric and polar and can exhibit some intriguing phenomena such as nonreciprocal superconductivity, chiral shift current, bulk photovoltaic effect, and exciton-polaritons. However, basic characterizations of individual TMD nanotubes are still quite limited, and much remains unclear about their structural chirality and electronic properties. Here we report an optical second-harmonic generation (SHG) study on multiwalled WS nanotubes on a single-tube level. As it is highly sensitive to the crystallographic symmetry, SHG microscopy unveiled multiple structural domains within a single WS nanotube, which are otherwise hidden under conventional white-light optical microscopy. Moreover, the polarization-resolved SHG anisotropy patterns revealed that different domains on the same tube can be of different chirality. In addition, we observed the excitonic states of individual WS nanotubes via SHG excitation spectroscopy, which were otherwise difficult to acquire due to the indirect band gap of the material.
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http://dx.doi.org/10.1021/acs.nanolett.1c00497DOI Listing
June 2021

A Combination of Geniposide and Chlorogenic Acid Combination Ameliorates Nonalcoholic Steatohepatitis in Mice by Inhibiting Kupffer Cell Activation.

Biomed Res Int 2021 13;2021:6615881. Epub 2021 May 13.

Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

The incidence of nonalcoholic steatohepatitis (NASH) is increasing worldwide. Activation of Kupffer cells (KCs) is central to the development of diet-induced NASH. We investigated whether a combination of two active chemical components, geniposide and chlorogenic acid (GC), at a specific ratio (67 : 1), ameliorates diet-induced NASH and the underlying mechanisms involved. C57BL/6J mice exposed to a high-fat and high-cholesterol (HFHC) diet containing cholesterol, choline, and high-sugar drinking water, as well as RAW264.7 cells stimulated with lipopolysaccharide (LPS) were studied. The combination exerted a therapeutic effect on HFHC-induced NASH in mice. Simultaneously, GC was found to reduce the expression of cytokines secreted by hepatic macrophages, including tumor necrosis factor- (TNF-), interleukin-1 (IL-1), IL-1, IL-6, monocyte chemotactic protein 1 (MCP-1), and granulocyte-macrophage colony-stimulating factor (GM-CSF). Moreover, GC reduced the number of KCs expressing F4/80. Furthermore, TNF-, inducible nitric oxide synthase (INOS), IL-1, and IL-6 mRNA and TNF- protein expression levels were suppressed upon GC treatment in RAW264.7 cells. Our findings suggest that GC has a strong anti-inflammatory effect in NASH, and this effect can be attributed to the suppression of KC activity in the liver.
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http://dx.doi.org/10.1155/2021/6615881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140849PMC
May 2021

Molecular Mechanisms and Current Pharmacotherapy of Peyronie's Disease: A Review.

Front Pharmacol 2021 20;12:643641. Epub 2021 May 20.

Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.

Peyronie's disease (PD) is a localized fibrotic lesion of the penis that has adverse effects on men's health. In this review, we summarized the molecular mechanisms and pharmacotherapies of PD. A literature search was conducted using PubMed and Cochrane Library during 2001-2020. Although no oral or topical medication demonstrated efficacy in monotherapy of PD, several intralesional medications have yielded promising results. Currently, the effective strategy in management of PD should be combined modality therapy, including but not limited to pharmacotherapy, mechanical therapy, and psychotherapy. Meanwhile, basic research is still necessary to facilitate the development of novel and more reliable treatments. In future, more attention should be given simultaneously to epigenetic changes, inflammatory cytokines, the abnormal wound-healing process, and profibrotic and anti-fibrotic factors to provide more options for this refractory disease.
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http://dx.doi.org/10.3389/fphar.2021.643641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173627PMC
May 2021

Rapid and sensitive detection of and based on bacitracin-modified [email protected] magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Anal Methods 2021 07;13(25):2804-2811

Department of Chemistry, Fudan University, Shanghai 200433, China.

The highly effective detection of pathogens in complex biological samples is an attractive and critical topic of study. Bacitracin is a novel broad-spectrum antimicrobial peptide to enrich bacteria via interactions with the membrane surface of the different bacterial cells. In this study, for the first time, bacitracin was immobilized on the surface of [email protected] magnetic nanoparticles for the enrichment of Staphylococcus aureus (G+) and Klebsiella pneumoniae (G-). Combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a rapid and sensitive detection method for these two bacteria was developed. In this method, the detectable concentration of bacteria was decreased by 2-3 orders of magnitude in unenriched samples. The enrichment and identification can be completed in one hour. All these results demonstrated that the bacitracin-functionalized magnetic composite has potential for use in the large-scale enrichment and isolation of target pathogens from complex biological samples, opening a new avenue for the rapid and sensitive detection of pathogens.
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http://dx.doi.org/10.1039/d1ay00614bDOI Listing
July 2021

Geniposide and Chlorogenic Acid Combination Improves Non-Alcoholic Fatty Liver Disease Involving the Potent Suppression of Elevated Hepatic SCD-1.

Front Pharmacol 2021 4;12:653641. Epub 2021 May 4.

Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Non-alcoholic fatty liver disease (NAFLD), characterized by the excessive accumulation of hepatic triglycerides (TGs), has become a worldwide chronic liver disease. But efficient therapy keeps unsettled. Our previous works show that geniposide and chlorogenic acid combination (namely the GC combination), two active chemical components combined with a unique ratio (67.16:1), presents beneficial effects on high-fat diet-induced NAFLD rodent models. Notably, microarray highlighted the more than 5-fold down-regulated SCD-1 gene in the GC combination group. SCD-1 is an essential lipogenic protein for monounsaturated fatty acids' biosynthesis and serves as a key regulatory enzyme in the last stage of hepatic lipogenesis (DNL). NAFLD mice model was fed with 16 weeks high-fat diet (HFD). The pharmacological effects, primarily on hepatic TG, TC, FFA, and liver enzymes, et al. of the GC combination and two individual components were evaluated. Furthermore, hepatic SCD-1 expression was quantified with qRT-PCR, immunoblotting, and immunohistochemistry. Finally, the lentivirus-mediated over-expressed cell was carried out to confirm the GC combination's influence on SCD-1. The GC combination could significantly reduce hepatic TG, TC, and FFA in NAFLD rodents. Notably, the GC combination presented synergetic therapeutic effects, compared with two components, on normalizing murine hepatic lipid deposition and disordered liver enzymes (ALT and AST). Meanwhile, the robust SCD-1 induction induced by HFD and FFA in rodents and ALM-12 cells was profoundly blunted, and this potent suppression was recapitulated in lentivirus-mediated SCD-1 over-expressed cells. Taken together, our data prove that the GC combination shows a substantial and synergetic anti-lipogenesis effect in treating NAFLD, and these amelioration effects are highly associated with the potent suppressed hepatic SCD-1 and a blunted DNL process.
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http://dx.doi.org/10.3389/fphar.2021.653641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129574PMC
May 2021

A meta-analysis of randomized controlled trials of a traditional Chinese medicine prescription, modified RunChang-Tang, in treating functional constipation.

Medicine (Baltimore) 2021 May;100(20):e25760

Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu.

Background: Modified Runchang-Tang (MRCT), a Chinese herbal medicine, is widely used to treat functional constipation (FC), which is a common digestive system disease. However, its efficacy has not been evaluated systematically and objectively. Thus, a meta-analysis was conducted to assess the efficacy and safety of MRCT for treating functional constipation.

Methods: We searched for relevant publications from Embase, Medline, The Cochrane Library, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Chinese Scientific Journals Database, and Wanfang Data for relevant literature. The timeframe of retrieval was set from the founding date of each database to July 15, 2020.

Result: A total of 26 randomized controlled trials with 2103 individuals were included in this meta-analysis. All trials were conducted in mainland China and were written in Chinese. The results showed that MRCT monotherapy provided better symptom relief in FC patients compared to prokinetic agent monotherapy (odds ratio, [OR] = 4.06), osmotic laxatives (OR = 4.39) and stimulant laxatives (OR = 2.99). Additionally, there were no obvious adverse effects in MRCT group compared with control group.

Conclusion: MRCT treatment is an efficient and safe treatment for FC. However, considering the limitations of this study, further well-designed randomized controlled trials are required to validate this conclusion.
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http://dx.doi.org/10.1097/MD.0000000000025760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137032PMC
May 2021

General Surface-Enhanced Raman Spectroscopy Method for Actively Capturing Target Molecules in Small Gaps.

J Am Chem Soc 2021 May 14;143(20):7769-7776. Epub 2021 May 14.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, Fujian 361005, P. R. China.

Over the past decade, many efforts have been devoted to designing and fabricating substrates for surface-enhanced Raman spectroscopy (SERS) with abundant hot spots to improve the sensitivity of detection. However, there have been many difficulties involved in causing molecules to enter hot spots actively or effectively. Here, we report a general SERS method for actively capturing target molecules in small gaps (hot spots) by constructing a nanocapillary pumping model. The ubiquity of hot spots and the inevitability of molecules entering them lights up all the hot spots and makes them effective. This general method can realize the highly sensitive detection of different types of molecules, including organic pollutants, drugs, poisons, toxins, pesticide residues, dyes, antibiotics, amino acids, antitumor drugs, explosives, and plasticizers. Additionally, in the dynamic detection process, an efficient and stable signal can be maintained for 1-2 min, which increases the practicality and operability of this method. Moreover, a dynamic detection process like this corresponds to the processes of material transformation in some organisms, so the method can be used to monitor transformation processes such as the death of a single cell caused by photothermal stimulation. Our method provides a novel pathway for generating hot spots that actively attract target molecules, and it can achieve general ultratrace detection of diverse substances and be applied to the study of cell behaviors in biological systems.
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http://dx.doi.org/10.1021/jacs.1c02169DOI Listing
May 2021

Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells.

Ann Transl Med 2021 Apr;9(8):628

Anhui Province Key Laboratory of Medical Physics and Technology/Institute of Health and Medical Technology, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei, China.

Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels.

Methods: We established an acquired radioresistant cell line from its parental NF639 cell line (HER2-positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. RNA-sequencing (RNA-seq) was also used to reveal the potential regulating genes and molecular mechanisms associated with the acquired changed phenotypes. Real-time PCR was used to validate the results of RNA-seq.

Results: The NF639R cells exhibited increased radioresistance and enhanced activity of proliferation, migration and anti-apoptosis, but decreased basal ROS. Two main energy metabolism pathways, mitochondrial respiration and glycolytic, were also upregulated. Furthermore, 490 differentially expressed genes were identified by RNA-seq. Enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes showed many differently expressed genes were significantly enriched in cell morphology, proliferation, migration, anti-apoptosis, antioxidation, tumor stem cells and energy metabolism and the signaling cascades such as the transforming growth factor-β, Wnt, Hedgehog, vascular endothelial growth factor, retinoic acid-inducible gene I (RIG-I)-like receptor, Toll-like receptor and nucleotide oligomerization domain (NOD)-like receptor were significantly altered in NF639R cells.

Conclusions: In clinical radiotherapy, repeat radiotherapy for short-term recurrence of breast cancer may result in enhanced radioresistance and promote malignant progression. Our research provided hints to understand the tumor resistance to radiotherapy and recurrence with a worse prognosis following radiotherapy.
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http://dx.doi.org/10.21037/atm-20-4667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106033PMC
April 2021

Surgical treatment for cryptoglandular and Crohn's perianal fistulas: Protocol of an umbrella review.

PLoS One 2021 13;16(5):e0251460. Epub 2021 May 13.

Department of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Introduction: A high prevalence of cryptoglandular and Crohn's perianal fistulas has been reported worldwide, and several surgical options are available for the management of anal fistula, with varying clinical efficacy. However, currently, the available evidence for the effectiveness of these surgical approaches are lacking and of concern in terms of the credibility and strength. The purpose of this study is to evaluate the credibility of the published systematic reviews and meta-analyses that assess the efficacy and safety of the surgical options for cryptoglandular and Crohn's perianal fistulas through an umbrella review.

Methods And Analysis: A systematic search in PubMed, Embase and Cochrane library will be performed from inception to December 2020 without any language restriction. We will include systematic reviews and meta-analyses that investigate the efficacy and safety of surgical approaches in the management of cryptoglandular and Crohn's perianal fistulas. Two reviewers will independently screen search results through reading the titles or abstracts. Relevant information will be extracted from each eligible systematic review or meta-analysis. Based on random effects model summary estimates along with their p values, 95% prediction intervals, between-study heterogeneity, small-study effects and excess significance, we will classify the evidence from convincing (class I) to weak (class IV). Findings will be summarized using quantitative synthesis combined with a narrative approach. Cryptoglandular and Crohn's perianal fistulas will be summarized separately. Two authors will independently perform the literature search, data extraction, and quality assessment of each included systematic review and meta-analysis. Any unresolved conflicts or doubts will be resolved by discussion or by consulting a senior author. The risk of bias of the systematic reviews will be assessed using a 16-item Assessment of Multiple Systematic Reviews 2 (AMSTAR2) checklist. The strength of evidence for the included systematic reviews will be classified as "high", "moderate", "low", or "critically low" quality.

Ethics And Dissemination: Ethics approval is not required as we will collect data from the published systematic reviews and meta-analyses without using individual patient data. The results of this umbrella review will be published in a peer-reviewed journal and will be presented at an anorectal disease conference.

Prospero Registration Number: CRD42020200754.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251460PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118242PMC
May 2021

Current applications of artificial intelligence combined with urine detection in disease diagnosis and treatment.

Transl Androl Urol 2021 Apr;10(4):1769-1779

Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.

In recent years, the advantages of artificial intelligence (AI) in data processing and model analysis have emerged in the medical field, enabled by computer technology developments and the integration of multiple disciplines. The application of AI in the medical field has gradually deepened and broadened. Among them, the development of clinical medicine intelligent decision-making is the fastest. The advantage of clinical medicine intelligent decision-making is to make the diagnosis faster and more accurate on the basis of certain information. Urine detection technologies, such as urine proteomics, urine metabolomics, and urine RNomics, have developed rapidly with the advancements in omics and medical tests. Advances in urine testing have made it possible to obtain a wealth of information from easily accessible urine. However, it has always been a problem to extract effective information from this information and use it. AI technology provides the possibility to process and use the information in urine. AI, combined with urine detection, not only provides new possibilities for precise and individual diagnosis and disease treatment, but also helps promote non-invasive diagnosis and treatment. This article reviews the research and applications of AI combined with urine detection for disease diagnosis and treatment and discusses its existing problems and future development.
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http://dx.doi.org/10.21037/tau-20-1405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100834PMC
April 2021

Long-Term Stability of Different Kinds of Gas Nanobubbles in Deionized and Salt Water.

Materials (Basel) 2021 Apr 6;14(7). Epub 2021 Apr 6.

College of Resources, Environment and Materials, Guangxi University, Nanning 530004, China.

Nanobubbles have many potential applications depending on their types. The long-term stability of different gas nanobubbles is necessary to be studied considering their applications. In the present study, five kinds of nanobubbles (N, O, Ar + 8%H, air and CO) in deionized water and a salt aqueous solution were prepared by the hydrodynamic cavitation method. The mean size and zeta potential of the nanobubbles were measured by a light scattering system, while the pH and Eh of the nanobubble suspensions were measured as a function of time. The nanobubble stability was predicted and discussed by the total potential energies between two bubbles by the extended Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. The nanobubbles, except CO, in deionized water showed a long-term stability for 60 days, while they were not stable in the 1 mM (milli mol/L) salt aqueous solution. During the 60 days, the bubble size gradually increased and decreased in deionized water. This size change was discussed by the Ostwald ripening effect coupled with the bubble interaction evaluated by the extended DLVO theory. On the other hand, CO nanobubbles in deionized water were not stable and disappeared after 5 days, while the CO nanobubbles in 1 mM of NaCl and CaCl aqueous solution became stable for 2 weeks. The floating and disappearing phenomena of nanobubbles were estimated and discussed by calculating the relationship between the terminal velocity of the floating bubble and bubble size.
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http://dx.doi.org/10.3390/ma14071808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038778PMC
April 2021

An effective oxygen content detection in phosphorescence of PtOEP-C6/Poly (St-co-TFEMA).

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 19;257:119786. Epub 2021 Apr 19.

Division of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China.

The phosphorescence of PtOEP-C6/Poly (St-co-TFEMA) has been investigated to achieve an accurate oxygen content, which is always puzzled as its extreme temperature sensitivity. The relations of oxygen content and phosphorescence intensity ratio can be perfectly fitted by the Stern-Volmer equation at different temperatures, meanwhile the monotonic quenching constant K is obtained, which enables us to develop a method of temperature correction to realize the intrinsic oxygen content. Then a clear fundamental picture of the temperature quenching mechanism of PtOEP is drawn by the time-resolved spectroscopy, the temperature sensitivity of phosphorescence arises from the enhanced quenching effect of oxygen by temperature. Our results provide an effective method to gain accurate oxygen content by simple optical measurement.
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http://dx.doi.org/10.1016/j.saa.2021.119786DOI Listing
August 2021

PET/Ag NW/PMMA transparent electromagnetic interference shielding films with high stability and flexibility.

Nanoscale 2021 May;13(17):8067-8076

College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.

Flexible transparent electromagnetic interference (EMI) shielding materials in visual windows are essential for the innovation of optoelectronic devices. Herein, we demonstrate the fabrication of flexible EMI shielding films based on silver nanowires (Ag NWs) and polymethyl methacrylate (PMMA). The purified Ag NWs are uniform in size and morphology, with aspect ratio over 1000. The PET/Ag NW/PMMA flexible transparent conductive films (FTCFs) with a sandwich structure were obtained via Mayer-rod coating of the Ag NWs and spin-coating of the PMMA polymer on a polyethylene terephthalate (PET) substrate. The root mean square roughness value of PET/Ag NW/PMMA FTCFs could decrease from 21 nm to 4 nm due to the filling of PMMA in the interface among Ag NWs. The PET/Ag NW/PMMA FTCFs can achieve a sheet resistance of 21 Ω sq-1 at a transmittance of 95.6%, resulting in a high figure of merit of 447. Moreover, the composite films exhibit remarkable flexibility after 10 000 continuous bending cycles, as well as great stability in harsh environments of 80 °C/80% RH aging for 600 h. An EMI shielding effectiveness (SE) of 21.3 dB in combination with a high optical transmittance of 95.6% for the PET/Ag NW/PMMA FTCFs is sufficient to satisfy the requirement of commercial transparent EMI shielding applications. The absorption component of the SE was demonstrated to play a dominant role in the EMI shielding mechanism. The comprehensive performance (flexibility, stability, transparency and EMI shielding performance) makes the PET/Ag NW/PMMA FTCFs have great potential as a flexible transparent EMI shielding material in emerging flexible optoelectronic devices.
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http://dx.doi.org/10.1039/d1nr00977jDOI Listing
May 2021

Integrative metabolic profile of myelodysplastic syndrome based on UHPLC-MS.

Biomed Chromatogr 2021 Sep 17;35(9):e5136. Epub 2021 May 17.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.

Myelodysplastic syndrome (MDS) is a neoplastic disease originating from hematopoietic stem cells. Currently, hematopoietic stem cell transplantation (HSCT) is the most effective cure, although lenalidomide, azacytidine, and decitabine have been applied to relieve symptoms of MDS. The purpose of this study was to evaluate the changes in endogenous metabolites by applying a UHPLC-MS (ultra-high-performance liquid chromatography-MS) metabolomics approach and to investigate metabolic pathways related to MDS. An untargeted metabolomics approach based on UHPLC-MS in combination with multivariate data analysis, including partial least squares discrimination analysis and orthogonal partial least squares discriminant analysis, was established to investigate potential biomarkers in the plasma of MDS patients. As a result, 29 biomarkers were identified to distinguish between MDS patients, HSCT patients, and healthy controls, which were mainly related to inflammation regulation, amino acid metabolism, fatty acid metabolism, and energy metabolism. To our knowledge, this is the first time where plasma metabolomics was combined with HSCT to study the pathogenesis and therapeutic target of MDS. The identification of biomarkers and analysis of metabolic pathways could offer the possibility of discovering new therapeutic targets for MDS in the future.
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http://dx.doi.org/10.1002/bmc.5136DOI Listing
September 2021

MiR-105 enhances osteogenic differentiation of hADSCs via the targeted regulation of SOX9.

Tissue Cell 2021 Oct 2;72:101540. Epub 2021 Apr 2.

Department of Endocrinology, Qinghai University Affiliated Hospital, Xining, Qinghai, China. Electronic address:

Objective: To investigate whether miR-105 can regulate the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs) by targeting SOX9.

Methods: The hADSCs were grouped for subsequent transfection and induction of osteogenic differentiation as follows: control, miR-NC, miR-105 mimics, miR-105 inhibitors, SOX9, SOX9 siRNA, miR-105 mimics + SOX9 and miR-105 inhibitors + SOX9 siRNA groups. Next, hADSCs were stained for alkaline phosphatase (ALP), and Alizarin Red S staining (ARS) was performed. Osteogenic differentiation-related genes and miR-105 expression were assessed by qRT-PCR, while SOX9 protein expression was determined by Western blotting.

Results: MiR-105 expression was increased and SOX9 protein expression was decreased during the osteogenic differentiation of hADSCs. A dual-luciferase reporter assay confirmed SOX9 to be a target gene of miR-105. Compared with the control group, the miR-105 mimics and SOX9 siRNA groups had elevated BMP2, OPN, OCN, BSP, Osx and Runx2 mRNA expression with reduced SOX9 expression, as well as increased ARS intensity and ALP activity. After transfection of miR-105 inhibitors/SOX9 into hADSCs, the results were the opposite. Overexpressing SOX9 reversed the effect of miR-105 in promoting the osteogenic differentiation of hADSCs.

Conclusion: MiR-105 could target SOX9 to improve the expression of osteogenic differentiation genes and thus enhance the osteogenic differentiation of hADSCs.
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http://dx.doi.org/10.1016/j.tice.2021.101540DOI Listing
October 2021

Tailor-made deep eutectic solvents extraction combined with UPLC-MS/MS determination of icarrin and icarisid II in rat plasma and its comparative pharmacokinetic application.

J Pharm Biomed Anal 2021 May 31;199:114054. Epub 2021 Mar 31.

School of Pharmacy, Shenyang Pharmaceutical University, Benxi, 117004, China. Electronic address:

Using green and high efficient solvents to extract and trace active ingredients of traditional Chinese medicine (TCM) in the complex biological samples was still challenging. In this paper, a co-friendly, fast pretreatment method with high extraction efficiency, based on the tailor-made deep eutectic solvent (DES) system, combined with ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination of icarrin and icarisid II in rat plasma samples, which can be further applied to comparative pharmacokinetic studies after oral administration of Herba Epimedii and icarrin monomer in rats, respectively. PrE (l-proline: ethylene glycol = 1:4 mol/mol) and acetonitrile were optimized and combined as the tailor-made DES at the volumetric ratio of 3:7 to extract icarrin and icarisid II, and to precipitate the protein in rat plasma in one step simultaneously. The extraction efficiency of the tailor-made DES was about 1.7 times of DES (PrE). The extraction recovery of icarrin and icarisid II in rat plasma samples by this method were within the range of 90-110 %, and the lower limits of quantification (LLOQ) were 0.32 ng mL (icarrin) and 0.43 ng mL (icarisid II). There was a linear relationship between 0.32-80.16 ng mL (icarrin) and 0.43-107.4 ng mL (icarisid II), which effectively reduced the detection limit. In this comparative pharmacokinetic study, the maximum plasma concentration (C) and the area under plasma concentration-time curve (AUC) of two analytes in rat plasma of Herba Epimedii group were both much higher than those in the icarrin monomer group, which suggested that other ingredients in Herba Epimedii may contribute to the in vivo absorption of icarrin and icarisid II. This simple, rapid, relatively green and high effeicient method would provide a new approach for the extraction of active ingredients from complex biological samples.
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http://dx.doi.org/10.1016/j.jpba.2021.114054DOI Listing
May 2021

Depressive males have higher odds of lower urinary tract symptoms suggestive of benign prostatic hyperplasia: a retrospective cohort study based on propensity score matching.

Asian J Androl 2021 Apr 2. Epub 2021 Apr 2.

Andrology Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China.

Lower urinary tract symptoms suggestive of benign prostate hyperplasia (LUTS/BPH) and depression are both increasing in Chinese aging males. However, the relationship still remains unknown. To explore their relationship, a retrospective cohort study based on propensity score matching (PSM) was conducted by analyzing the China Health and Retirement Longitudinal Study dataset. After data cleaning, a total of 5125 participants were enrolled and subjected to PSM; 1351 pairs were matched and followed for 2 years. Further logistic regression and restricted cubic spline (RCS) were performed to evaluate, model and visualize the relationship between depression and LUTS/BPH. Moreover, subgroup analyses and sensitivity analyses were adopted to verify the robustness of the conclusions. Before PSM, depressive patients showed higher odds of LUTS/BPH in all three models adjusting for different covariates (P < 0.001). After PSM, univariate logistic regression revealed that depressive patients had higher risks for LUTS/BPH than participants in the control group (odds ratio [OR] = 2.10, P < 0.001). The RCS results indicated a nonlinear (P < 0.05) and inverted U-shaped relationship between depression and LUTS/BPH. In the subgroup analyses, no increased risks were found among participants who were not married or cohabitating, received an education, had an abnormal body mass index (<18.5 kg m and ≥28 kg m), slept more than 6 h, did not smoke, and drank less than once a month (all P > 0.05). The results of sensitivity analyses indicated identical increased risks of LUTS/BPH in all four models (all P < 0.001). In conclusion, depression enhances the risks of LUTS/BPH in aging males.
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http://dx.doi.org/10.4103/aja.aja_12_21DOI Listing
April 2021

Serum Vitamin D Level and the Risk of Urinary Tract Infection in Children: A Systematic Review and Meta-Analysis.

Front Public Health 2021 19;9:637529. Epub 2021 Mar 19.

Department of Pediatrics, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

This systematic review and meta-analysis aimed to evaluate the association between serum vitamin D concentration and the risk of urinary tract infection (UTI) in children. Human studies reported the serum vitamin D level in children with UTI and healthy controls were collected from PubMed, Scopus, Embase, and Cochrane databases. The strictly standardized mean difference (SSMD) and 95% confidence interval (CI) were calculated to evaluate the relationship between serum vitamin D levels and risk of UTI. The results of analysis showed that serum vitamin D levels in children with UTI were significantly lower than healthy control children (SSMD: 0.891, 95% CI: 0.707-1.075, < 0.000; SSMD: 0.797, 95% CI: 0.500-1.094, < 0.000, respectively). It can be concluded that there is a significant negative relationship between serum vitamin D level and risk of UTI in children.
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http://dx.doi.org/10.3389/fpubh.2021.637529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017300PMC
May 2021

Application of magnetic resonance fingerprinting to differentiate grade I transitional and fibrous meningiomas from meningothelial meningiomas.

Quant Imaging Med Surg 2021 Apr;11(4):1447-1457

Department of Medical Imaging, Zhengzhou University People's Hospital and Henan Provincial People's Hospital, Zhengzhou, China.

Background: The choice of surgical treatment for meningiomas is affected by the subtype and clinical characteristics. Therefore, an accurate preoperative diagnosis is essential. Current magnetic resonance imaging (MRI) technology is unable to distinguish between meningioma subtypes. In the present study, we compared and evaluated the utility of conventional MRI, magnetic resonance fingerprinting (MRF), and diffusion-weighted imaging (DWI) in differentiating World Health Organization grade I transitional and fibrous meningiomas from meningothelial meningiomas.

Methods: Forty-six patients with pathologically confirmed meningiomas (15 meningothelial, 18 transitional, and 13 fibrous) were enrolled in the present study. All patients underwent conventional MRI, MRF, and DWI scans before surgery using a 3T scanner. The Jonckheere-Terpstra test was used to analyze differences in the signal and enhancement characteristics of the three groups from T-weighted imaging (T1WI) and T-weighted imaging (T2WI). To investigate the difference in quantitative T1 and T2 values derived from MRF and apparent diffusion coefficient (ADC) values between the three groups using the Kruskal-Wallis test, regions of interest (ROIs) were manually drawn on the parenchymal portion of the tumors; P<0.017 was considered statistically significant after Bonferroni correction for multiple comparison. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic performances of the different parameters.

Results: Meningothelial meningiomas had significantly higher T1 and T2 values than transitional and fibrous meningiomas (all P<0.017). ROC analysis results revealed that the combination of T1 and T2 values had the largest area under the curve (AUC). The AUC for the combination of T1 and T2 values was 0.826 between meningothelial and transitional meningiomas, and the AUC for the combination of T1 and T2 values between meningothelial and fibrous meningiomas was 0.903. No significant differences were found in the T1 and T2 values between transitional and fibrous meningiomas. There were also no statistically significant differences in the conventional MRI (including T1WI, T2WI, and contrast-enhanced T1WI) and ADC values between the three meningioma subtypes (all P>0.05).

Conclusions: MRF may provide more quantitative information than either conventional MRI or DWI for differentiating transitional and fibrous meningiomas from meningothelial meningiomas. T1 and T2 values derived from MRF may distinguish transitional and fibrous meningiomas from meningothelial meningiomas, and the combination of T1 and T2 values provides the highest diagnostic efficacy.
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http://dx.doi.org/10.21037/qims-20-732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930661PMC
April 2021

Manipulating ZmEXPA4 expression ameliorates the drought-induced prolonged anthesis and silking interval in maize.

Plant Cell 2021 Jul;33(6):2058-2071

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

Drought poses a major environmental threat to maize (Zea mays) production worldwide. Since maize is a monoecious plant, maize grain yield is dependent on the synchronous development of male and female inflorescences. When a drought episode occurs during flowering, however, an asynchronism occurs in the anthesis and silking interval (ASI) that results in significant yield losses. The underlying mechanism responsible for this asynchronism is still unclear. Here, we obtained a comprehensive development-drought transcriptome atlas of maize ears. Genes that function in cell expansion and growth were highly repressed by drought in 50 mm ears. Notably, an association study using a natural-variation population of maize revealed a significant relationship between the level of α-expansin4 (ZmEXPA4) expression and drought-induced increases in ASI. Furthermore, genetic manipulation of ZmEXPA4 expression using a drought-inducible promoter in developing maize ears reduced the ASI under drought conditions. These findings provide important insights into the molecular mechanism underlying the increase in ASI in maize ears subjected to drought and provide a promising strategy that can be used for trait improvement.
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http://dx.doi.org/10.1093/plcell/koab083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290287PMC
July 2021
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