Publications by authors named "Feng Li"

5,707 Publications

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L-arginine stimulates the proliferation of mouse mammary epithelial cells and the development of mammary gland in pubertal mice by activating the GPRC6A/PI3K/AKT/mTOR signalling pathway.

J Anim Physiol Anim Nutr (Berl) 2022 May 26. Epub 2022 May 26.

Department of Theoretic Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.

Amino acids have been shown to affect the development of mammary gland (MG). However, it is unclear whether L-arginine promotes the development of pubertal MG. Therefore, our study aims to explore the effect of L-arginine on the development of MG in pubertal mice. To investigate its internal mechanism of action, we will use mouse mammary epithelial cells (mMECs) line. Whole-mount staining showed that L-arginine can promote the extension of MG duct. In vitro, 0.4 mM L-arginine could activate the G protein-coupled receptor family C, group 6, subtype A (GPRC6A)/phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signalling pathway and increase the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (4EBP1) to promote the synthesis of cell cycle regulatory protein D1 (Cyclin D1), leading to the dissociation of the retinoblastoma tumour suppressor protein (Rb)-E2F1 transcription factor (E2F1) complex in mMECs and releasing E2F1 to promote cell proliferation. Furthermore, GPRC6A was knocked down or inhibition of the PI3K/AKT/mTOR signalling pathway with corresponding inhibitors completely abolished the arginine-induced promotion of mMECs proliferation. In vivo, it was further confirmed that 0.1% L-arginine can activate the PI3K/AKT/mTOR signalling pathway in the MG of pubertal mice. These results were able to indicate that L-arginine stimulates the development of MG in pubertal mice through the GPRC6A/PI3K/AKT/mTOR signalling pathway.
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http://dx.doi.org/10.1111/jpn.13730DOI Listing
May 2022

An Interlayer Containing Dissociated LiNO with Fast Release Speed for Stable Lithium Metal Batteries with 400 Wh kg Energy Density.

Small 2022 May 26:e2202349. Epub 2022 May 26.

Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China.

Lithium metal is an ideal electrode material for future rechargeable batteries. However, dendrite formation and unstable solid electrolyte interphase film lead to safety concerns and poor Coulombic efficiency (CE). LiNO significantly improves the performance of the lithium metal anode in ester electrolytes but its use is restricted by low solubility. To increase the content of LiNO in the cell, a poly-(vinyl carbonate) organogel interlayer containing dissociated LiNO (LNO-PVC) is placed between the cathode and anode. The dissociated LiNO effectively increases the LiNO -release rate and compensates for the LiNO consumed in ester electrolytes during cycling. Via this interlayer, the performance of the lithium metal anode is significantly improved. The average CE of a Li-Cu cell reaches 98.6% at 0.5 mA cm -1 h and 98.5% at 1 mA cm -1 h for 300 cycles. Also, a Li||NCM811 pouch cell with LNO-PVC interlayer can also reach a 400 Wh kg energy density with a cycling life of 65 cycles. This strategy sheds light on the effect of the state of this salt on its release/dissolution kinetics, which is determined by the interactions between the salt and host material.
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http://dx.doi.org/10.1002/smll.202202349DOI Listing
May 2022

The prognostic value of the preoperative inflammatory index on the survival of glioblastoma patients.

Neurol Sci 2022 May 23. Epub 2022 May 23.

Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Cheeloo College of Medicine, Shandong University, Wenhua Xi Road, Jinan, 250012, China.

Objectives: The growth and development of tumors are closely related to the initiation and amplification of the inflammatory response. Various inflammatory biomarkers had attained growing attention for nearly two decades and were discovered strongly associated with cancer patients' prognosis, indicating that systemic inflammatory response is possibly essential to cancer progression. However, little was known about the sensitive biomarkers associated with the detection, persistence, treatment, and prognosis of GBM. Hence, the retrospective research endeavored to evaluate the prognostic value of preoperative inflammatory biomarkers in patients with GBM who initially received standardized treatment.

Methods: The 232 glioblastoma patients eligible who were admitted to Qilu Hospitals in Shandong Province from January 2014 to January 2018 were collected for this analysis. Inflammatory markers, including the systemic immune-inflammation index (SII), systemic immune response index (SIRI), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), monocyte-lymphocyte ratio (MLR), and albumin/globulin ratio (AGR), were designed. Progression-free survival (PFS) and overall survival (OS) were estimated using the Kaplan-Meier method, and we calculated the area under the ROC curve to determine the AUC value. Besides, we used the Cox proportional hazard model to estimate the relationship between variables and PFS and OS. The statistical differences between variables and PFS and OS were tested through the log-rank test. What is more, the LR method was used to perform Cox multiple regression analysis. The results were represented by hazard ratio (HR), 95% CI, any 2-tailed P < 0.01 was accepted as statistically different.

Results: The multivariate Cox proportional hazard model presented that SII ≥ 659.1 was an independent risk factor affecting OS (HR = 2.238, 95% CI = 1.471-3.406, P < 0.001) and postoperative PFS (HR = 2.000, 95% CI = 1.472-2.716, P < 0.001) in GBM patients. The 1-, 3-, and 5-year OS of the SII < 659.1 group was 70.8%, 26.9%, and 14.1%, respectively, while the 1- and 3-year OS of the SII ≥ 659.1 group was 37.5% and 11.5% (P < 0.001). The 1-, 3-, and 5-year PFS of the SII < 659.1 group was 36.3%, 19.6%, and 13%, respectively, while the 1-year PFS of the SII ≥ 659.1 group was 11.3% (P < 0.001). Results of patients' clinical and pathological characteristics paraded that in comparison to the lower SII group, the higher SII group had significantly inferior Karnofsky Performance Scale (KPS) scores (P < 0.001) and more frequent cystic changes of the tumors (P < 0.001), whereas the values of SIRI, NLR, PLR, MLR, and AGR were low.

Conclusions: SII is an independent inflammatory indicator for predicting the prognosis of GBM patients after receiving initially standardized treatments.
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http://dx.doi.org/10.1007/s10072-022-06158-wDOI Listing
May 2022

Application of MR Imaging Features in Differentiation of Renal Changes in Patients With Stage III Type 2 Diabetic Nephropathy and Normal Subjects.

Front Endocrinol (Lausanne) 2022 4;13:846407. Epub 2022 May 4.

Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, China.

Objective: The objective of the study was to explore the value of MRI texture features based on T1WI, T2-FS and diffusion-weighted imaging (DWI) in differentiation of renal changes in patients with stage III type 2 diabetic nephropathy (DN) and normal subjects.

Materials And Methods: A retrospective analysis was performed to analyze 44 healthy volunteers (group A) and 40 patients with stage III type 2 diabetic nephropathy (group B) with microalbuminuria. Urinary albumin to creatinine ratio (ACR) <30 mg/g, estimated glomerular filtration rate (eGFR) in the range of 60-120 ml/(min 1.73 m), and randomly divided into primary cohort and test cohort. Conventional MRI and DWI of kidney were performed using 1.5 T magnetic resonance imaging (MRI). The outline of the renal parenchyma was manually labeled in fat-suppressed T2-weighted imaging (FS-T2WI), and PyRadiomics was used to extract radiomics features. The radiomics features were then selected by the least absolute shrinkage and selection operator (LASSO) method.

Results: There was a significant difference in sex and body mass index (BMI) (P <0.05) in the primary cohort, with no significant difference in age. In the final results, the wavelet and Laplacian-Gaussian filtering are used to extract 1,892 image features from the original T1WI image, and the LASSO algorithm is used for selection. One first-order feature and six texture features are selected through 10 cross-validations. In the mass, 1,638 imaging extracts features from the original T2WI image.1 first-order feature and 5 texture features were selected. A total of 1,241 imaging features were extracted from the original ADC images, and 5 texture features were selected. Using LASSO-Logistic regression analysis, 10 features were selected for modeling, and a combined diagnosis model of diabetic nephropathy based on texture features was established. The average unit cost in the logistic regression model was 0.98, the 95% confidence interval for the predictive efficacy was 0.9486-1.0, specificity 0.97 and precision 0.93, particularly. ROC curves also revealed that the model could distinguish with high sensitivity of at least 92%.

Conclusion: In consequence, the texture features based on MR have broad application prospects in the early detection of DN as a relatively simple and noninvasive tool without contrast media administration.
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http://dx.doi.org/10.3389/fendo.2022.846407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114464PMC
May 2022

Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip-Powered CRISPR/Cas12a System.

Small 2022 May 22:e2200854. Epub 2022 May 22.

State Key Laboratory of Membrane Biology, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.

The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. The high resource consumption of coronavirus disease 2019 (COVID-19) is resulting in insufficient surveillance of coinfection or resurgence of other critical respiratory epidemics, which is of public concern. To facilitate evaluation of the current coinfection situation, a microfluidic system (MAPnavi) is developed for the rapid (<40 min) and sensitive diagnosis of multiple respiratory viruses from swab samples in a fully sealed and automated manner, in which a nested-recombinase polymerase amplification and the CRISPR-based amplification system is first proposed to ensure the sensitivity and specificity. This novel system has a remarkably low limit of detection (50-200 copies mL ) and is successfully applied to detect 171 clinical samples (98.5% positive predictive agreement; 100% negative predictive agreement), and the results identify 45.6% coinfection among clinical samples from patients with COVID-19. This approach has the potential to shift diagnostic and surveillance efforts from targeted testing for a high-priority virus to comprehensive testing of multiple virus sets and to greatly benefit the implementation of decentralized testing.
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http://dx.doi.org/10.1002/smll.202200854DOI Listing
May 2022

Reply to the letter to editor regarding, "Global incidence, prevalence, and disability of vertebral fractures: a systematic analysis of the global burden of disease study 2019".

Authors:
Yimin Dong Feng Li

Spine J 2022 Jun;22(6):1071

Department of orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

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http://dx.doi.org/10.1016/j.spinee.2022.02.016DOI Listing
June 2022

Novel microRNA biomarkers of systemic lupus erythematosus in plasma: miR-124-3p and miR-377-3p.

Clin Biochem 2022 May 19. Epub 2022 May 19.

Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address:

Background: To explore the clinical value of miR-124-3p and miR-377-3p in plasma, and to examine whether these microRNAs can serve as biomarkers for systemic lupus erythematosus.

Methods: Samples from 50 patients with SLE and 47 healthy individuals were collected from the Rheumatology and Immunology Department at the Second Affiliated Hospital of Chongqing Medical University. The expression of miR-124-3p and miR-377-3p in peripheral blood mononuclear cells (PBMCs) and serum were determined by quantitative reverse-transcription PCR (RT-PCR). We then further analyzed the associations between these microRNAs and clinically relevant indicators. We employed receiver operating characteristic (ROC) curves and the area under the ROC curves (AUCs) to validate the diagnostic value of miR-124-3p and miR-377-3p.

Results: The expression of miR-124-3p and miR-377-3p was significantly upregulated in PBMCs and serum from SLE patients compared with the normal control group (P < 0.05). ROC curve analysis indicated that plasma miR-124-3p and miR-377-3p were thus candidate diagnostic biomarkers for SLE, with AUCs of 0.714 (95% CI, 0.610 to 0.820, P < 0.05) and 0.705 (95% CI, 0.600 to 0.809, P < 0.05), respectively. Furthermore, the combined diagnostic efficiency of miR-124-3p and miR-377-3p was higher than that for miR-124-3p or miR-377-3p alone, and the AUCs for miR-124-3p and miR-377-3p in plasma were higher than in PBMCs. Plasma miR-124-3p expression was also associated with various clinicopathological parameters such as antiC1q and C3. Binary logistic regression analysis revealed that the expression of miR-377-3p was an independent predictor for SLE, and the plasma miR-124-3p was independently associated with the remission rate of SLE.

Conclusion: Circulating miR-124-3p and miR-377-3p may constitute promising biomarkers for the diagnosis of SLE.
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http://dx.doi.org/10.1016/j.clinbiochem.2022.05.004DOI Listing
May 2022

Functional characterization and comparison of lycopene epsilon-cyclase genes in Nicotiana tabacum.

BMC Plant Biol 2022 May 21;22(1):252. Epub 2022 May 21.

College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China.

Background: Lycopene epsilon-cyclase (ε-LCY) is a key enzyme in the carotenoid biosynthetic pathway (CBP) of higher plants. In previous work, we cloned two Ntε-LCY genes from allotetraploid tobacco (Nicotiana tabacum), Ntε-LCY2 and Ntε-LCY1, and demonstrated the overall effect of Ntε-LCY genes on carotenoid biosynthesis and stress resistance. However, their genetic and functional characteristics require further research in polyploid plants.

Results: Here, we used CRISPR/Cas9 to obtain Ntε-LCY2 and Ntε-LCY1 mutants in allotetraploid N.tabacum K326. Ntε-LCY2 and Ntε-LCY1 had similar promoter cis-acting elements, including light-responsive elements. The Ntε-LCY genes were expressed in roots, stems, leaves, flowers, and young fruit, and their highest expression levels were found in leaves. Ntε-LCY2 and Ntε-LCY1 genes responded differently to normal light and high light stress. Both the Ntε-LCY2 and the Ntε-LCY1 mutants had a more rapid leaf growth rate, especially ntε-lcy2-1. The expression levels of CBP genes were increased in the ntε-lcy mutants, and their total carotenoid content was higher. Under both normal light and high light stress, the ntε-lcy mutants had higher photosynthetic capacities and heat dissipation levels than the wild type, and this was especially true of ntε-lcy2-1. The reactive oxygen species content was lower in leaves of the ntε-lcy mutants.

Conclusion: In summary, the expression patterns and biological functions of the Ntε-LCY genes Ntε-LCY1 and Ntε-LCY2 differed in several respects. The mutation of Ntε-LCY2 was associated with a greater increase in the content of chlorophyll and various carotenoid components, and it enhanced the stress resistance of tobacco plants under high light.
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http://dx.doi.org/10.1186/s12870-022-03634-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123772PMC
May 2022

CORO1C, a novel PAK4 binding protein, recruits phospho-PAK4 serine 99 to the leading edge and promotes the migration of gastric cancer cells.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia formation, recruits cytoplasmic PAK4 to the leading edge of gastric cancer cells by C-terminal extension (CE) domain of CORO1C The localization of PAK4 on the leading edge of the cell depends on two necessary conditions: the phosphorylation of PAK4 on serine 99 and the binding to the CE domain of CORO1C. Unphosphorylated PAK4 on serine 99 is closely associated with microtubules by PAK4/GEF-H1/Tctex-1 complex. Once phosphorylated, PAK4 is released from microtubule, and then is recruited by CORO1C to the leading edge and regulates the CORO1C/RCC2 (regulator of chromosome condensation 2) complex, leading to the migration of gastric cancer cells. Our results reveal a new mechanism by which PAK4 regulates the migration potential of gastric cancer cells through microtubule-microfilament cross talk.
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http://dx.doi.org/10.3724/abbs.2022044DOI Listing
April 2022

Fourier Domain Mode Locked Laser and Its Applications.

Sensors (Basel) 2022 Apr 20;22(9). Epub 2022 Apr 20.

Shenzhen Research Institute, The Hong Kong Polytechnic University, Shenzhen 518057, China.

The sweep rate of conventional short-cavity lasers with an intracavity-swept filter is limited by the buildup time of laser signals from spontaneous emissions. The Fourier domain mode-locked (FDML) laser was proposed to overcome the limitations of buildup time by inserting a long fiber delay in the cavity to store the whole swept signal and has attracted much interest in both theoretical and experimental studies. In this review, the theoretical models to understand the dynamics of the FDML laser and the experimental techniques to realize high speed, wide sweep range, long coherence length, high output power and highly stable swept signals in FDML lasers will be discussed. We will then discuss the applications of FDML lasers in optical coherence tomography (OCT), fiber sensing, precision measurement, microwave generation and nonlinear microscopy.
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http://dx.doi.org/10.3390/s22093145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105910PMC
April 2022

Sea Urchin Polyketide Synthase SpPks1 Produces the Naphthalene Precursor to Echinoderm Pigments.

J Am Chem Soc 2022 May 19. Epub 2022 May 19.

Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.

Nearly every animal species on Earth contains a unique polyketide synthase (PKS) encoded in its genome, yet no animal-clade PKS has been biochemically characterized, and even the chemical products of these ubiquitous enzymes are known in only a few cases. The earliest animal genome-encoded PKS gene to be identified was 1 from sea urchins. Previous genetic knockdown experiments implicated 1 in synthesis of the sea urchin pigment echinochrome. Here, we express and purify , performing biochemical experiments to demonstrate that the sea urchin protein is responsible for the synthesis of 2-acetyl-1,3,6,8-tetrahydroxynaphthalene (ATHN). Since ATHN is a plausible precursor of echinochromes, this result defines a biosynthetic pathway to the ubiquitous echinoderm pigments and rewrites the previous hypothesis for echinochrome biosynthesis. Truncation experiments showed that, unlike other type I iterative PKSs so far characterized, produces the naphthalene core using solely ketoacylsynthase (KS), acyltransferase, and acyl carrier protein domains, delineating a unique class of animal nonreducing aromatic PKSs (aPKSs). A series of amino acids in the KS domain define the family and are likely crucial in cyclization activity. Phylogenetic analyses indicate that SpPks1 and its homologs are widespread in echinoderms and their closest relatives, the acorn worms, reinforcing their fundamental importance to echinoderm biology. While the animal microbiome is known to produce aromatic polyketides, this work provides biochemical evidence that animals themselves also harbor ancient, convergent, dedicated pathways to carbocyclic aromatic polyketides. More fundamentally, biochemical analysis of SpPks1 begins to define the vast and unexplored biosynthetic space of the ubiquitous animal PKS family.
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http://dx.doi.org/10.1021/jacs.2c01416DOI Listing
May 2022

Effects of Surgical Treatment Guided by the Three-Column Classification Method on Knee Joint Function and Postoperative Complications in Patients with Tibial Plateau Fractures.

Comput Intell Neurosci 2022 9;2022:5935102. Epub 2022 May 9.

Luoyang Orthopedic Hospital of Henan Province (Orthopedic Hospital of Henan Province), Luoyang 471002, Henan, China.

Many patients with tibial plateau fractures present with various degrees of soft tissue contusion and severely damaged articular surface, ligament, and vascular nerves, and thus how to treat this kind of patient has become one of the great difficulties in clinical practice. Therefore, we aim to investigate the effects of surgical treatment guided by the three-column classification method on knee joint function and postoperative complications in patients with tibial plateau fractures. A total of 120 patients with three-column tibial plateau fractures admitted to our hospital from January 2018 to January 2019 were selected and divided into group A ( = 60) and group B ( = 60). Among them, the group A patients were treated with an anterior lateral approach in floating positions combined with reduction plate internal fixation with an L-shaped approach in the posteromedial joint, while the patients in group B received reduction plate internal fixation with a knee midline incision in supine positions. After that, the perioperative indexes, knee function scores, the MOS item short-from health survey (SF-36) scores, complication rate (CR), and overall treatment efficacy of the patients were compared between the two groups. The perioperative indexes in group A were significantly better than those in group B ( < 0.001); the knee function scores and SF-36 scores in group A were significantly higher than those in group B ( < 0.001); the CR in group A was significantly lower than that in group B ( < 0.001); the treatment efficacy in group A was significantly better than that in group B ( < 0.05). The three-column classification method, with highly instructive significance in tibial plateau fracture surgery, can improve treatment efficacy and reduce the incidence of complications, which is worthy of application and promotion in clinical practice.
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http://dx.doi.org/10.1155/2022/5935102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110135PMC
May 2022

Construction of gastric cancer patient-derived organoids and their utilization in a comparative study of clinically used paclitaxel nanoformulations.

J Nanobiotechnology 2022 May 18;20(1):233. Epub 2022 May 18.

Department of Gastroenterology and Hepatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.

Background: Gastric cancer (GC) is a highly heterogeneous disease with many different histological and molecular subtypes. Due to their reduced systemic adverse effects, nanoformulation agents have attracted increasing attention for use in the treatment of GC patients in the clinic. To improve therapeutic outcomes, it is vitally necessary to provide individual medication references and guidance for use of these nanoformulations, and patient-derived organoids (PDOs) are promising models through which to achieve this goal.

Results: Using an improved enzymatic digestion process, we succeeded in constructing GC PDOs from surgically resected tumor tissues and endoscopic biopsies from GC patients; these PDOs closely recapitulated the histopathological and genomic features of the corresponding primary tumors. Next, we chose two representative paclitaxel (PTX) nanoformulations for comparative study and found that liposomal PTX outperformed albumin-bound PTX in killing GC PDOs at both the transcriptome and cellular levels. Our results further showed that the different distributions of liposomal PTX and albumin-bound PTX in PDOs played an essential role in the distinct mechanisms through which they kill PDOs. Finally, we constructed patient-derived xenografts model in which we verified the above distinct therapeutic outcomes via an intratumoral administration route.

Conclusions: This study demonstrates that GC PDOs are reliable tools for predicting nanoformulation efficacy.
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http://dx.doi.org/10.1186/s12951-022-01431-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118843PMC
May 2022

Singlet and triplet to doublet energy transfer: improving organic light-emitting diodes with radicals.

Nat Commun 2022 May 18;13(1):2744. Epub 2022 May 18.

Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge, CB3 0HE, UK.

Organic light-emitting diodes (OLEDs) must be engineered to circumvent the efficiency limit imposed by the 3:1 ratio of triplet to singlet exciton formation following electron-hole capture. Here we show the spin nature of luminescent radicals such as TTM-3PCz allows direct energy harvesting from both singlet and triplet excitons through energy transfer, with subsequent rapid and efficient light emission from the doublet excitons. This is demonstrated with a model Thermally-Activated Delayed Fluorescence (TADF) organic semiconductor, 4CzIPN, where reverse intersystem crossing from triplets is characteristically slow (50% emission by 1 µs). The radical:TADF combination shows much faster emission via the doublet channel (80% emission by 100 ns) than the comparable TADF-only system, and sustains higher electroluminescent efficiency with increasing current density than a radical-only device. By unlocking energy transfer channels between singlet, triplet and doublet excitons, further technology opportunities are enabled for optoelectronics using organic radicals.
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http://dx.doi.org/10.1038/s41467-022-29759-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117228PMC
May 2022

Virome analysis of ticks in Zhoushan Archipelago, China.

J Vet Med Sci 2022 May 17. Epub 2022 May 17.

Chinese People's Liberation Army Center for Disease Control and Prevention.

Ticks are an important group of arthropod vectors. Ticks pose a profound risk to public health by transmitting many types of microorganisms that are human and animal pathogens. With the development of next-generation sequencing (NGS) technology and viral metagenomics, numerous novel viruses have been discovered in ticks and tick-related hosts. To fully understand the virus spectrum in ticks in the Zhoushan Archipelago of Zhejiang province in China, ticks were collected from Qushan Island, Zhoushan Island, and Daishan Island in the Zhoushan Archipelago in June 2016. NGS performed to investigate the diversity of tick-associated viruses identified 21 viral sequences. Twelve were pathogenic to humans and animals. Trough verification by polymerase chain reaction (PCR) revealed the existence of three tick-associated viruses with extensive homology with Dabieshan, MG22, and Odaw virus. Other NGS-detected sequences that could not be amplified by PCR were highly homologous (92-100%) with known pathogenic viruses that included hepatitis B virus, papillomavirus, and human mastadenovirus C. This is the first study to systematically apply high throughput sequencing technology to explore the spectrum of viruses carried by ticks in the Zhoushan Archipelago. The findings are fundamental knowledge of the diversity of tick-associated viruses in this region and will inform strategies to monitor and prevent the spread of tick-borne diseases.
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http://dx.doi.org/10.1292/jvms.22-0058DOI Listing
May 2022

A Bionic Interface to Suppressing the Coffee-ring Effect for Reliable and Flexible Perovskite Modules with a near 90% Yield Rate.

Adv Mater 2022 May 18:e2201840. Epub 2022 May 18.

College of Chemistry and Chemical Engineering/Institute of Polymers and Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.

The inhomogeneity, poor interfacial contact and pin-holes caused by the coffee-ring effect severely affect the printing reliability of flexible perovskite solar cells (PSCs). Herein, inspired by the bio-glue of barnacles, a bionic interface layer (Bio-IL) of NiO /levodopa is introduced to suppress the coffee-ring effect during printing perovskite modules. The coordination effect of the sticky functional groups in Bio-IL can pinning the three-phase contact line and restrain the transport of perovskite colloidal particles during the printing and evaporation process. Moreover, the sedimentation rate of perovskite precursor is accelerated due to the electrostatic attraction and rapid volatilization from an extraordinary wettability. The super-hydrophilic Bio-IL affords an even spread over a large-area substrate, which boosts a complete and uniform liquid film for heterogeneous nucleation as well as crystallization. Perovskite films on different large-area substrates with negligible coffee-ring effect are printed. Consequently, the inverted flexible PSCs and perovskite solar modules achieve a high efficiency of 21.08% and 16.87%, respectively. This strategy ensures a highly reliable reproducibility of printing PSCs with a near 90% yield rate. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202201840DOI Listing
May 2022

A Chlorine-Based Redox Electrochemical Capacitor.

ACS Appl Mater Interfaces 2022 May 17. Epub 2022 May 17.

Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.

Electrochemical capacitors are under the spotlight due to their high power density, but they have a low energy density. Redox electrolytes have emerged as a promising approach to design high-energy electrochemical energy storage devices. Herein, a chlorine-based redox electrochemical capacitor is reported in an ionic liquid electrolyte. The commercial activated carbon is employed as the working electrode to render the reversible redox of chloride ions in an ionic liquid, by the restriction of micropores on neutral chlorine. The carbon material can simultaneously provide electrical double-layer capacitance. The effective integration of a chlorine redox reaction and electrical double layer allows for high-energy electrochemical capacitors. By this means, a rechargeable chlorine-based redox electrochemical capacitor with reversible capacity and good rate capability and cycling stability is obtained. This work offers a solution for a new type of high-energy electrochemical capacitors.
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http://dx.doi.org/10.1021/acsami.2c03951DOI Listing
May 2022

High mobility group protein B1 decreases surface localization of PD-1 to augment T-cell activation.

Cancer Immunol Res 2022 May 17. Epub 2022 May 17.

First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

High-mobility group protein B1 (HMGB1) is a danger signaling molecule that has been found to trigger an effective antitumor immune response. However, the mechanisms underlying its antitumor effects are not fully understood. Here, we found that HMGB1 release induced by chemotherapy in patients with non-small cell lung cancer (NSCLC) was negatively correlated with PD-1 expression on CD8+ T cells. In vitro analysis indicated that treatment with HMGB1 led to a significant decrease in the level of expression of PD-1 on CD8+ T cells. Further analysis demonstrated that HMGB1 reduced PD-1 expression by inducing dynamin-mediated internalization of the protein, leading to early endocytosis in the cytoplasm, and subsequently degradation in the lysosomes. In a xenograft model, HER2-targeted chimeric-antigen receptor (CAR) T cells had enhanced function in the presence of HMGB1. These data identify a role for HMGB1 as a negative regulator of PD-1 signaling in lung cancer cells and the observed antitumor effect of HMGB1 on chimeric-antigen receptor (CAR) T cells may provide a theoretical foundation for a new immunotherapy combination.
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http://dx.doi.org/10.1158/2326-6066.CIR-21-0652DOI Listing
May 2022

An Open, Prospective Cohort Study of VV116 in Chinese Participants Infected with SARS-CoV-2 Omicron Variants.

Emerg Microbes Infect 2022 May 17:1-22. Epub 2022 May 17.

Department of Respiratory Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Omicron variant of SARS-CoV-2 has become the predominant variant worldwide. VV116 is an oral drug with robust anti-SARS-CoV-2 efficacy in preclinical studies. We conducted an open, prospective cohort study to evaluate its safety and effectiveness in Chinese participants infected with SARS-CoV-2 omicron variant from March 8th, 2022 to Marth 24th, 2022. 136 patients hospitalized patients confirmed with COVID-19 were enrolled in this study, including 60 patients who received VV116 (300mg, BID×5 days) in the treatment group and 76 patients who didn't receive VV116 in the control group besides standard treatment. Viral load shedding time and adverse events were collected during the follow-up. There was no significant difference in baseline characteristics between the VV116 group and the control group, except for a higher prevalence of symptoms in the control group (P=0.021). The median time from the first positive test to the first VV116 administration was 5 (range: 2-10) days. Participants who received VV116 within 5 days since the first positive test had a shorter viral shedding time than the control group (8.56 vs 11.13 days), and cox regression analysis showed adjusted HR of 2.37 [95%CI 1.50-3.75], <0.001). In symptomatic subgroup participants, VV116 group had a shorter viral shedding time than the control group (=0.016). A total of 9 adverse events with no serious adverse events were reported in the VV116 group, 7 of which were mild liver function abnormalities, and all of them were resolved without intervention. VV116 is a safe, effective oral antiviral drug, which shows a better performance within the early onset of omicron infection. ClinicalTrials.gov NCT05242042 ClinicalTrials.gov NCT05279235 ClinicalTrials.gov NCT05341609
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http://dx.doi.org/10.1080/22221751.2022.2078230DOI Listing
May 2022

Deterministic Relation between Optical Polarization and Lattice Symmetry Revealed in Ion-Doped Single Microcrystals.

ACS Nano 2022 May 17. Epub 2022 May 17.

Key Laboratory for Physical Electronics and Devices of the Ministry of Education, School of Electronic Science and Engineering, Faculty of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Rare-earth ion doped crystals are of great significance for microsensing and quantum information, while the ions in the crystals emit light with spontaneous partial polarization, which is, though believed to be originated from the crystal lattice structure, still lacking a deterministic explanation that can be tested with quantitative accuracy. We report experimental evidence showing the profound physical relation between the polarization degree of light emitted by the doped ion and the lattice symmetry by demonstrating, with high precision, that the lattice constant ratio / directly quantifies the macroscopic effective polar angle of the electric and magnetic dipoles, which essentially determines the linear polarization degree of the emission. Based on this result, we further propose a pure optical technology to identify the three-dimensional orientation of a rod-shaped single microcrystal using the polarization-resolved microspectroscopy. Our results, demonstrating the physical origin of light polarization in ion-doped crystals, allow work toward on-demand polarization control with crystallography and provide a versatile platform for polarization-based microscale sensing in dynamical systems.
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http://dx.doi.org/10.1021/acsnano.2c02756DOI Listing
May 2022

Light modulation based on the enhanced Kerr effect in molybdenum disulfide nanostructures with curved features.

Phys Chem Chem Phys 2022 May 25;24(20):12208-12213. Epub 2022 May 25.

Key Laboratory of Physical Electronics and Devices of Ministry of Education & Shaanxi Key Laboratory of Information Photonic Technique, School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

A novel type of molybdenum disulfide (MoS) nanoparticles (NPs) was chemically synthesized, which possessed curved features with three-dimensional (3D) freedom compared with planar two-dimensional (2D) materials. Due to the introduction of curved features, the synthesized NPs exhibited a strongly enhanced nonlinear refractive index ( ∼ 10 cm W) and third-order susceptibility ( ∼ 10 esu), which were experimentally verified by the spatial self-phase modulation effect in the visible wavelength range. Both the nonlinear parameters were two orders of magnitude higher than their planar MoS nanostructure counterparts. In addition, the relative change of the effective nonlinear refractive index Δ/ was found to be distinctly dependent on the intensity of the applied electromagnetic field. Moreover, an all-optical modulation was experimentally realized based on the spatial cross-phase modulation effect. Our results demonstrate planar MoS materials with 3D features as potential candidates for next generation all-optical applications and open a substantial approach for the design of efficient nanomaterials with favorable optical nonlinearity.
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http://dx.doi.org/10.1039/d2cp00374kDOI Listing
May 2022

Corrigendum: Landscape Genomics in Tree Conservation Under a Changing Environment.

Authors:
Li Feng Fang K Du

Front Plant Sci 2022 28;13:911163. Epub 2022 Apr 28.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.

[This corrects the article DOI: 10.3389/fpls.2022.822217.].
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http://dx.doi.org/10.3389/fpls.2022.911163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097259PMC
April 2022

Association of marital status with cardiovascular outcome in patients with breast cancer.

J Thorac Dis 2022 Apr;14(4):841-850

Department of Cardiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.

Background: The influences of marital status on cardiovascular death risk in patients with breast cancer remained unclear. This study aimed to evaluate the associations of different marital status with cardiovascular death risk in patients with breast cancer.

Methods: A total of 182,666 female breast cancer patients were enrolled in this study from the Surveillance, Epidemiology, and End Results (SEER) database from 2010 to 2014, and was divided into two groups: married (N=107,043) and unmarried (N=75,623). A 1:1 propensity score matching (PSM) was applied to reduce inter-group bias between the two groups. Competing-risks model was used to assess the associations between different marital status and cardiovascular death risk in patients with breast cancer.

Results: After PSM, marital status was an independent predictor for cardiovascular death in patients with breast cancer. Unmarried condition was associated with increased cardiovascular death risk than married condition among breast cancer patients [unadjusted model: hazard ratio (HR) =2.012, 95% confidence interval (CI): 1.835-2.208, P<0.001; Model 1: HR =1.958, 95% CI: 1.785-2.148, P<0.001; Model 2: HR =1.954, 95% CI: 1.781-2.144, P<0.001; Model 3: HR =1.920, 95% CI: 1.748-2.107, P<0.001]. With the exception of separated condition (adjusted HR =0.886, 95% CI: 0.474-1.658, P=0.705), further unmarried subgroups analysis showed that the other three unmarried status were associated with increased cardiovascular death risk as follows: single (adjusted HR =1.623, 95% CI: 1.421-1.853, P<0.001), divorced (adjusted HR =1.394, 95% CI: 1.209-1.608, P<0.001), and widowed (adjusted HR =2.460, 95% CI: 2.227-2.717, P<0.001). In particularly, widowed condition showed the highest cardiovascular death risk in all 4 unmarried subgroups.

Conclusions: Unmarried condition (e.g., single, divorced and widowed) was associated with elevated cardiovascular death risk compared with their married counterparts in patients with breast cancer, suggesting that more attention and humanistic care should be paid to unmarried breast cancer patients (especially the widowed patients) in the management of female breast cancer patients.
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http://dx.doi.org/10.21037/jtd-21-1261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096314PMC
April 2022

Aerospace Technology Improves Fermentation Potential of Microorganisms.

Front Microbiol 2022 29;13:896556. Epub 2022 Apr 29.

Department of Computer Science, University of California, Irvine, Irvine, CA, United States.

It is highly possible to obtain high-quality microbial products in appreciable amounts, as aerospace technology is advancing continuously. Genome-wide genetic variations in microorganisms can be triggered by space microgravity and radiation. Mutation rate is high, mutant range is wide, and final mutant character is stable. Therefore, space microorganism breeding is growing to be a new and promising area in microbial science and has greatly propelled the development of fermentation technology. Numerous studies have discovered the following improvements of fermentation potential in microorganisms after exposure to space: (1) reduction in fermentation cycle and increase in growth rate; (2) improvement of mixed fermentation species; (3) increase in bacterial conjugation efficiency and motility; (4) improvement of the bioactivity of various key enzymes and product quality; (5) enhancement of multiple adverse stress resistance; (6) improvement of fermentation metabolites, flavor, appearance, and stability. Aerospace fermentation technology predominantly contributes to bioprocessing in a microgravity environment. Unlike terrestrial fermentation, aerospace fermentation keeps cells suspended in the fluid medium without significant shear forces. Space radiation and microgravity have physical, chemical, and biological effects on mutant microorganisms by causing alternation in fluid dynamics and genome, transcriptome, proteome, and metabolome levels.
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http://dx.doi.org/10.3389/fmicb.2022.896556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106405PMC
April 2022

Identification of the Phosphorus-Solubilizing Bacteria Strain JP233 and Its Effects on Soil Phosphorus Leaching Loss and Crop Growth.

Front Microbiol 2022 29;13:892533. Epub 2022 Apr 29.

Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, China.

Phosphorus (P) is one of the most limiting nutrients in global agricultural ecosystems, and phosphorus-solubilizing bacteria (PSB) can convert insoluble P into soluble P, thereby improving the absorption and use of soil P by plants. Increasing leaching loss of soil P due to PSB that could lead to water eutrophication is a major concern, although no direct experimental evidence is available to evaluate these effects. In this study, a highly efficient PSB strain, sp. JP233, was isolated from soil and its P-solubilizing agent was identified by metabolomics and HPLC analyses. The effects of JP233 on P contents in soil leachates were also analyzed by microcosm leaching experiments in the absence and presence of maize. JP233 could solubilize insoluble P into soluble forms, and the molybdate reactive phosphorus (MRP) content reached 258.07 mg/L in NBRIP medium containing 5 g/L Ca(PO) within 48 h. Metabolomics analysis demonstrated that the organic acid involved in JP233 P solubilization was primarily 2-keto gluconic acid (2KGA). Further, HPLC analysis revealed that 2KGA contents rapidly accumulated to 19.33 mg/mL within 48 h. Microcosm leaching experiments showed that MRP and total phosphorus (TP) contents in soil leaching solutions were not significantly higher after JP233 inoculation. However, inoculation with JP233 into maize plant soils significantly decreased MRP and TP contents in the soil leaching solutions on days 14 ( < 0.01), 21 ( < 0.01), and 28 ( < 0.05). Inoculation with strain JP233 also significantly increased the biomass of maize aerial components and that of whole plants ( < 0.05). Thus, strain JP233 exhibited a significant plant-growth-promoting effect on maize development. In conclusion, the application of PSB into soils does not significantly increase P leachate loss. Rather, the application of PSB can help reduce P leachate loss, while significantly promoting plant absorption and use of soil P.
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http://dx.doi.org/10.3389/fmicb.2022.892533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100411PMC
April 2022

The Long-Term Outcomes of Ablation With Vein of Marshall Ethanol Infusion vs. Ablation Alone in Patients With Atrial Fibrillation: A Meta-Analysis.

Front Cardiovasc Med 2022 29;9:871654. Epub 2022 Apr 29.

Department of Cardiology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.

Background: The long-term outcomes of ablation with vein of Marshall ethanol infusion (VOM-ABL) compared with ablation alone in patients with atrial fibrillation (AF) remains elusive. We aimed to explore whether VOM-ABL showed better long-term benefits and screen the potential determinants of outcome impact of VOM-ABL procedure.

Methods: PubMed, Cochrane Library, Web of Science, and Embase were searched up to 1st September 2021. Studies comparing the long-term (one-year or longer) outcomes between VOM-ABL and ablation alone were included. Subgroup analysis identified potential determinants for VOM-ABL procedure.

Results: Compared with ablation alone, VOM-ABL was associated with a significantly higher rate of long-term freedom from AF/AT (risk ratio [RR], 1.28; 95% confidence interval [CI], 1.12-1.47; = 0.00) and successful mitral isthmus (MI) block (RR, 1.52; 95% CI, 1.16-1.99; = 0.00), whereas, there was no significant difference in pericardial effusion, stroke/transient ischemic attack (TIA), and all-cause death. Subgroup analysis identified two significant treatment-covariate interactions: one was ablation strategy subgroup (pulmonary vein isolation plus linear and/or substrate ablation [PVI+]; RR, 1.41; 95% CI, 1.27-1.56 vs. PVI; RR, 1.05; 95% CI, 0.92-1.19, = 0.00 for interaction) for freedom from AF/AT, while the other was VOM-ABL group sample size subgroup (≥ 100; RR, 1.98; 95% CI, 1.24-3.17 vs. <100; RR, 1.20; 95% CI, 1.10-1.30, = 0.04 for interaction) for MI block.

Conclusions: This meta-analysis demonstrates that VOM-ABL has superior efficacy and comparable safety over ablation alone in AF patients with long-term follow-up. Moreover, PVI+ and VOM-ABL group sample size ≥ 100 may be associated with a great impact on freedom from AF/AT and MI block, respectively.
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http://dx.doi.org/10.3389/fcvm.2022.871654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098965PMC
April 2022

Mitigation of DSS-Induced Colitis Potentially via Th1/Th2 Cytokine and Immunological Function Balance Induced by Phenolic-Enriched Buckwheat ( Moench) Bee Pollen Extract.

Foods 2022 Apr 29;11(9). Epub 2022 Apr 29.

College of Food Science and Technology, Northwest University, Xi'an 710069, China.

Colitis is an inflammatory disease that results from the overactivation of effector immune cells, producing a high quantity of pro-inflammatory cytokines. Our study aimed to explore whether buckwheat () bee pollen extract (FBPE) could inhibit the progression of dextran sulfate sodium (DSS)-induced colitis via regulating immune function. We isolated and identified six main phenolic compounds of FBPE such as luteolin (9.46 mg/g) by column chromatography, HPLC-DAD, ESI-MS and NMR spectroscopy, then assessed their effects on colonic mucosal injury by clinical symptoms, histomorphology and immunohistochemistry examinations. The results showed that FBPE at 25.2 g/kg body weight (g/kg BW) changed the clinical symptoms of colitis, the ICAM-1 expression in colon, the activity of related inflammatory mediators in colon tissue and helped restore the immune system. Compared with the model group (40.28%), the CD4 positivity was significantly reduced in the HD (High-dose group: 25.2 g FBPE/kg BW/day) group (20.45%). Administration of 25.2 g/kg BW of FBPE decreased the IFN-γ, TNF-α and IL-4 levels, while enhancing the IL-10 level, and significantly inhibited the abnormally decreased IgG (Model: 13.25 mg/mL, HD: 14.06 mg/mL), showing a reversal effect on the Th1/Th2 levels in colitis. These findings suggested that FBPE at 25.2 g/kg BW had the effects of alleviating colitis and immunomodulation, which can help in the development of safe and effective immune therapy.
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http://dx.doi.org/10.3390/foods11091293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105923PMC
April 2022

Integrated Multiomics Analysis of Salivary Exosomes to Identify Biomarkers Associated with Changes in Mood States and Fatigue.

Int J Mol Sci 2022 May 9;23(9). Epub 2022 May 9.

The Drug Discovery Lab, Department of Neurology, David Geffen School of Medicine, 710 Westwood Plaza, University of California Los Angeles, Los Angeles, CA 90095, USA.

Fatigue and other deleterious mood alterations resulting from prolonged efforts such as a long work shift can lead to a decrease in vigilance and cognitive performance, increasing the likelihood of errors during the execution of attention-demanding activities such as piloting an aircraft or performing medical procedures. Thus, a method to rapidly and objectively assess the risk for such cognitive fatigue would be of value. The objective of the study was the identification in saliva-borne exosomes of molecular signals associated with changes in mood and fatigue that may increase the risk of reduced cognitive performance. Using integrated multiomics analysis of exosomes from the saliva of medical residents before and after a 12 h work shift, we observed changes in the abundances of several proteins and miRNAs that were associated with various mood states, and specifically fatigue, as determined by a Profile of Mood States questionnaire. The findings herein point to a promising protein biomarker, phosphoglycerate kinase 1 (PGK1), that was associated with fatigue and displayed changes in abundance in saliva, and we suggest a possible biological mechanism whereby the expression of the PGK1 gene is regulated by miR3185 in response to fatigue. Overall, these data suggest that multiomics analysis of salivary exosomes has merit for identifying novel biomarkers associated with changes in mood states and fatigue. The promising biomarker protein presents an opportunity for the development of a rapid saliva-based test for the assessment of these changes.
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http://dx.doi.org/10.3390/ijms23095257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105576PMC
May 2022

Nitric Oxide Enhanced Salt Stress Tolerance in Tomato Seedlings, Involving Phytohormone Equilibrium and Photosynthesis.

Int J Mol Sci 2022 Apr 20;23(9). Epub 2022 Apr 20.

College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, China.

Nitric oxide (NO), as a ubiquitous gas signaling molecule, modulates various physiological and biochemical processes and stress responses in plants. In our study, the NO donor nitrosoglutathione (GSNO) significantly promoted tomato seedling growth under NaCl stress, whereas NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide potassium (cPTIO) treatment reversed the positive effect of NO, indicating that NO plays an essential role in enhancing salt stress resistance. To explore the mechanism of NO-alleviated salt stress, the transcriptome of tomato leaves was analyzed. A total of 739 differentially expressed genes (DEGs) were identified and classified into different metabolic pathways, especially photosynthesis, plant hormone signal transduction, and carbon metabolism. Of these, approximately 16 and 9 DEGs involved in plant signal transduction and photosynthesis, respectively, were further studied. We found that GSNO increased the endogenous indoleacetic acid (IAA) and salicylic acid (SA) levels but decreased abscisic acid (ABA) and ethylene (ETH) levels under salt stress conditions. Additionally, GSNO induced increases in photosynthesis pigment content and chlorophyll fluorescence parameters under NaCl stress, thereby enhancing the photosynthetic capacity of tomato seedlings. Moreover, the effects of NO mentioned above were reversed by cPTIO. Together, the results of this study revealed that NO regulates the expression of genes related to phytohormone signal transduction and photosynthesis antenna proteins and, therefore, regulates endogenous hormonal equilibrium and enhances photosynthetic capacity, alleviating salt toxicity in tomato seedlings.
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http://dx.doi.org/10.3390/ijms23094539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102644PMC
April 2022

MiR-34a-5p/Sirt1 axis: A novel pathway for puerarin-mediated hepatoprotection against benzo(a)pyrene.

Free Radic Biol Med 2022 May 10;186:53-65. Epub 2022 May 10.

College of Food Science and Engineering, Shandong Agricultural University, Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, Taian, 271018, People's Republic of China. Electronic address:

Benzo[a]pyrene (BaP) as a carcinogen induces oxidative stress and inflammation, causing health problems including liver damage. Puerarin (a natural flavonoid) is traditionally used to provide hepatoprotective effects. This research was established to meet the rising demand for effective therapies/treatments against hepatic diseases and investigate the mechanism underlying the protective actions of puerarin against BaP-induced liver damage. In mice, puerarin combated effectively the detrimental changes in liver weight, color and function indices caused by BaP. In HepG2 cells, puerarin alleviated BaP-induced cell death, oxidative stress and inflammation, and such effects were positively correlated with puerarin's concentration (12.5-50 μM). Mechanistic studies revealed that BaP induced low Sirt1 expression and high miR-34a-5p expression, and puerarin treatment alleviated these changes. Oxidative stress and inflammation induced by BaP were almost eliminated when miR-34a-5p was silenced. Inhibiting miR-34a-5p or overexpressing Sirt1 had a similar effect to puerain treatment. Overexpression of miR-34a-5p and inhibition of Sirt1 reduced the protective effect of puerarin. Collectively, miR-34a-5p participates in the regulation of puerarin's protective function against BaP-induced injury through targeting Sirt1. There is a novel pathway for suppressing oxidative stress and inflammation via miR-34a-5p/Sirt1 axis in puerarin-mediated hepatoprotection, which opens up a new avenue for alternative therapies.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.05.006DOI Listing
May 2022
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