Publications by authors named "Feng Jiang"

1,386 Publications

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Thermal Radiation Driven Ultrafast Crystallization of Perovskite Films Under Heavy Humidity for Efficient Inverted Solar Cells.

Adv Mater 2022 Aug 3:e2205143. Epub 2022 Aug 3.

Department of materials science and Engineering, Southern University of Science and Technology, Shenzhen, Guangdong Province, 518055, China.

Fabricating perovskite solar cells (PSCs) in air is conducive to low-cost commercial production, nevertheless it is rather difficult to achieve comparable device performance as that in an inert atmosphere because of poor moisture toleration of perovskite materials. Here, the perovskite crystallization process was systematically studied using two-step sequential solution deposition in inert atmosphere (glovebox) and air. We found that moisture can stabilize solvation intermediates and prevent their conversion into perovskite crystals. To address this issue, we used thermal radiation to accelerate perovskite crystallization for integrated perovskite films within 10 s in air. The as-formed perovskite films were compact, highly oriented with giant grain size, superior photoelectric properties, and low trap density. When the films were applied to PSC devices, we obtained a champion power conversion efficiency of 20.8%, one of the best results for air-processed inverted PSCs under high relative humidity (60±10%). This work substantially assists understanding and modulation to perovskite crystallization kinetics under heavy humidity. Also, the ultrafast conversion strategy by thermal radiation provides unprecedented opportunities to manufacture high-quality perovskite films for low-temperature, eco-friendly, and air-processed efficient inverted PSCs. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202205143DOI Listing
August 2022

A novel steady-state model to quantitively assess the effect of pH elevation by dissimilatory sulfate reduction process in acidic waters in mining areas.

Water Res 2022 Jul 12;222:118852. Epub 2022 Jul 12.

Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation Technology, School of Environmental Science & Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Acidic waters such as groundwater, drainage and lakes in mining area contain high-strength acids and metal ions, posing serious threats to aquatic ecosystems and human health. Dissimilatory sulfate reduction (DSR)-based processes are attractive technologies for remediating acidic waters because it produces alkalinity and sulfide for metal precipitation and acid neutralization. However, the effects of pH elevation achieved by DSR-based processes are case-sensitive and difficult to be quantitively assessed, which limits the application of DSR process for acidic water remediation. Therefore, in this study, a Sulfidogenic Acid mine water Remediation Model (SARM) considering the DSR process, weak acids balance, metal sulfide and hydroxide precipitations, and gas-liquid exchanges of HS and CO, was developed to quantitatively assess the effects of various environmental factors on the pH elevation by a DSR process in acidic waters. A long-term trial of a DSR reactor was conducted to calibrate and validate the SARM. The experimental results revealed that the DSR-based process is effective to relieve acidity. The calibrated SARM demonstrated the excellent performance to predict the pH variation in the DSR reactor, under the varied conditions of influent pH and organic concentration. The calibrated SARM was further validated with data collected from literatures, and the results verified that the proposed model is capable to accurately assess the effect of DSR process on acid neutralization and metal removals under various conditions in steady state. The model was employed to systematically evaluate the impacts of environmental factors on acid remediation within a DSR-based process. The results revealed that the background alkalinity plays an important role in acid neutralization. However, with an increase in sulfate reduction, biogenic sulfide and carbonate become the dominant buffering substances to neutralize acidity. Furthermore, the SARM was used to evaluate the applicability of the DSR-based process for the remediation of acidic waters by evaluating the sulfide production thresholds for acid neutralization and metal removal. The simulation results demonstrated that, the DSR-based process is recommended for the remediation of acidic waters with low background alkalinity. Collectively, the SARM proposed in this study was found to be a useful and efficient tool for quantitatively assessing the potential of DSR-based processes for neutralizing acidic waters, which is vital for biogeochemistry and environmental engineering research.
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http://dx.doi.org/10.1016/j.watres.2022.118852DOI Listing
July 2022

Peracetic acid activation by mechanochemically sulfidated zero valent iron for micropollutants degradation: Enhancement mechanism and strategy for extending applicability.

Water Res 2022 Jul 21;222:118887. Epub 2022 Jul 21.

State Key Laboratory of Hydraulics and Mountain River Engineering, College of Architecture and Environment, Sichuan University, Chengdu 610065, China; Sino-German Centre for Water and Health Research, Sichuan University, Chengdu 610065, China. Electronic address:

In this study, mechanically sulfidated microscale zero valent iron (S-ZVI) was found to effectively activate the peracetic acid (PAA) with a result of almost complete degradation of six micropollutants within 10 min under neutral conditions, and > 95% sulfamethoxazole (SMX) removal after six cycles. Reactive oxidized species (ROS) including HO•, carbon-centered radicals, and Fe(IV) were generated in the S-ZVI/PAA system, while HO• was the main contributor towards micropollutants degradation. This study clearly revealed that enhancement of the electron donating ability of ZVI by the formed conductive iron sulfides was crucial for promoted Fe(II) generation and subsequent PAA activation over several cycles, rather than the ability of sulfides to reduce Fe(III) for Fe(II) regeneration as reported previously. Interestingly, it's discovered that co-existence of Fe(III) would dramatically improve the contaminants removal efficiency of the S-ZVI/PAA system; transform the surfaced Fe(II) dominated ROS generation process to aqueous Fe(II) one; enhance the tolerance of the proposed system to water matrix. The promoting effect of predosed Fe(III) on PAA activation by S-ZVI should be mainly associated with: the greater ability of Fe(III) than HO to accept electron from Fe for obtaining more active sites; slower Fe consumption and solid sulfur species release for elevated electron utilization efficiency and PAA activation. Considering the convenient and cost-effective access of Fe(III), the decrease of acute toxicity of treated SMX, excellent stability and good removal of various micropollutants fully demonstrate the superiority of S-ZVI/PAA system for practical application.
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http://dx.doi.org/10.1016/j.watres.2022.118887DOI Listing
July 2022

ARIMA and ARIMA-ERNN models for prediction of pertussis incidence in mainland China from 2004 to 2021.

BMC Public Health 2022 07 29;22(1):1447. Epub 2022 Jul 29.

School of Public Health, Fudan University, Shanghai, 200032, China.

Objective: To compare an autoregressive integrated moving average (ARIMA) model with a model that combines ARIMA with the Elman recurrent neural network (ARIMA-ERNN) in predicting the incidence of pertussis in mainland China.

Background: The incidence of pertussis has increased rapidly in mainland China since 2016, making the disease an increasing public health threat. There is a pressing need for models capable of accurately predicting the incidence of pertussis in order to guide prevention and control measures. We developed and compared two models for predicting pertussis incidence in mainland China.

Methods: Data on the incidence of pertussis in mainland China from 2004 to 2019 were obtained from the official website of the Chinese Center for Disease Control and Prevention. An ARIMA model was established using SAS (ver. 9.4) software and an ARIMA-ERNN model was established using MATLAB (ver. R2019a) software. The performances of these models were compared.

Results: From 2004 to 2019, there were 104,837 reported cases of pertussis in mainland China, with an increasing incidence over time. The incidence of pertussis showed obvious seasonal characteristics, with the peak lasting from March to September every year. Compared with the mean squared error (MSE), mean absolute error (MAE), and mean absolute percentage error (MAPE) of the ARIMA model, those of the ARIMA-ERNN model were 81.43%, 95.97% and 80.86% lower, respectively, in fitting performance. In terms of prediction performance, the MAE, MSE and MAPE were 37.75%, 56.88% and 43.75% lower, respectively.

Conclusion: The fitting and prediction performances of the ARIMA-ERNN model were better than those of the ARIMA model. This provides theoretical support for the prediction of infectious diseases and should be beneficial to public health decision making.
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http://dx.doi.org/10.1186/s12889-022-13872-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338508PMC
July 2022

[Separation and enrichment of trace aflatoxin B in grains by magnetic nanomaterials based on [email protected]].

Se Pu 2022 Aug;40(8):694-703

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

In this study, a magnetic nanomaterial antibody (Ab)[email protected] was synthesized, which was employed to absorb aflatoxin B (AFB) in complicated grain matrices. The [email protected] material was then paired with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for subsequent accurate detection. The [email protected] material has a specific adsorption capacity for AFB because of the stable and specific biological binding between antigen and antibody. This process can achieve the identification between the material and food matrix quickly, thereby completing the separation and enrichment process. Then, high sensitivity and high accuracy HPLC-MS/MS were employed for signal readout and actual quantification, which can significantly increase the detection efficiency and enable high-throughput detection of numerous samples. In the pretreatment process, FeO was first synthesized by microwave-assisted hydrothermal synthesis within 1 h, and [email protected] was then produced using the enhanced Stober's approach. This material with high adsorption performance was synthesized under relatively mild conditions and short time. To obtain [email protected] materials with uniform particle size, magnetic properties, and dispersibility that met the requirements, synthesis conditions of [email protected] and conditions for capturing the AFB target were analyzed. The findings demonstrated that the best effect was obtained when the dosage of FeCl·6HO was 10.0 mmol, the heating time was 40 min, and 100 μL tetraethoxysilane was employed for SiO coating. The AFB antibody was then combined with the surface of [email protected] under several conditions. The findings revealed that the best coupling efficiency of Ab could be obtained when the concentration of 2-morpholinoethanesulfonic acid monohydrate (MES) was 10 mmol/L, pH was 6.5, and the molar ratio of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)∶-hydroxysuccinimide substances (NHS) was 2∶1. The coupling buffer was then selected as phosphate buffer (PBS) with pH=7.4, and 8 mg [email protected] was employed to separate and enrich AFB at 37 ℃ for 10 min. In the actual detection, acetonitrile-water-formic acid (85∶10∶5, v/v/v) was employed as the extraction solution. After ultrasonic extraction for 10 min, [email protected] was employed to separate and enrich AFB in the extract. The supernatant was dried with nitrogen and reconstituted with 1-mL acetonitrile. The solution was then filtered through a 0.22 μm filter and detected using HPLC-MS/MS, thereby realizing the quick and quantitative detection of AFB. AFB had an excellent linear relationship in the range of 2-50 μg/L under the optimal analytical conditions, and the correlation coefficient was less than 0.99. The LOD was 0.04 μg/kg, and the LOQ was 0.13 μg/kg. The spiked recoveries of AFB in three grain matrices ranged from 76.21% to 92.85% with RSD≤5.29% at four different spiked levels. The approach was applied to the determination and analysis of AFB in 30 real grain samples of rice, corn, and wheat. The findings demonstrated that AFB was detected in one wheat sample and two corn samples, and its content was 0.38, 0.13, and 0.47 μg/kg, respectively, and no toxins were found in other samples. The approach combined [email protected] magnetic nanomaterials with HPLC-MS/MS, which could obtain high-efficiency separation and enrichment of AFB. Furthermore, the low-cost [email protected] could be stored for more than a week and complete the pretreatment process within 30 min. This effective pretreatment process combined with HPLC-MS/MS could realize the analysis of several samples within a short time, and had a promising application prospect in the detection of AFB in grains.
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http://dx.doi.org/10.3724/SP.J.1123.2022.03002DOI Listing
August 2022

Characterization of Fatty Acid Metabolism-Related Genes Landscape for Predicting Prognosis and Aiding Immunotherapy in Glioma Patients.

Front Immunol 2022 12;13:902143. Epub 2022 Jul 12.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Glioma is a highly malignant brain tumor with a poor survival rate. The involvement of fatty acid metabolism in glioma was examined to find viable treatment options. The information was gathered from the Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA) databases. A prognostic signature containing fatty acid metabolism-dependent genes (FAMDs) was developed to predict glioma outcome by multivariate and most minor absolute shrinkage and selection operator (LASSO) regression analyses. In the TCGA cohort, individuals with a good score had a worse prognosis than those with a poor score, validated in the CGGA cohort. According to further research by "pRRophetic" R package, higher-risk individuals were more susceptible to crizotinib. According to a complete study of the connection between the predictive risk rating model and tumor microenvironment (TME) features, high-risk individuals were eligible for activating the immune cell-associated receptor pathway. We also discovered that anti-PD-1/PD-L1 and anti-CTLA4 immunotherapy are more effective in high-risk individuals. Furthermore, we demonstrated that CCNA2 promotes glioma proliferation, migration, and invasion and regulates macrophage polarization. Therefore, examining the fatty acid metabolism pathway aids our understanding of TME invasion properties, allowing us to develop more effective immunotherapies for glioma.
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http://dx.doi.org/10.3389/fimmu.2022.902143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315048PMC
August 2022

Microarray-Based Prediction of Polycythemia after Exposure to High Altitudes.

Genes (Basel) 2022 Jul 2;13(7). Epub 2022 Jul 2.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.

In high-altitude environments, the prevalence of high-altitude polycythemia (HAPC) ranges between 5 and 18 percent. However, there is currently no effective treatment for this condition. Therefore, disease prevention has emerged as a critical strategy against this disease. Here, we looked into the microarray profiles of GSE135109 and GSE29977, linked to either short- or long-term exposure to the Qinghai Tibet Plateau (QTP). The results revealed inhibition in the adaptive immune response during 30 days of exposure to QTP. Following a gene set enrichment analysis (GSEA) discovered that genes associated with HAPC were enriched in Cluster1, which showed a dramatic upregulation on the third day after arriving at the QTP. We then used GeneLogit to construct a logistic prediction model, which allowed us to identify 50 genes that classify HAPC patients. In these genes, and were also significantly altered following early QTP exposure, suggesting that they may serve as hub genes for HAPC development. The in-depth study of a combination of the datasets of transcriptomic changes during exposure to a high altitude and whether diseases occur after long-term exposure in Hans can give us some inspiration about genes associated with HAPC development during adaption to high altitudes.
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http://dx.doi.org/10.3390/genes13071193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316656PMC
July 2022

Eco-Friendly Extraction, Structure, and Gel Properties of ι-Carrageenan Extracted Using Ca(OH).

Mar Drugs 2022 Jun 27;20(7). Epub 2022 Jun 27.

College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.

An eco-friendly method for ι-carrageenan extraction from seaweed through boiling and using a low concentration of Ca(OH) is reported. Compared to the traditional method of ι-carrageenan extraction using NaOH, the reported method using Ca(OH) had the advantages of using 93.3% less alkali and 86.8% less water, having a 25.0% shorter total extraction time, a 17.6% higher yield, and a 43.3% higher gel strength of the product. In addition, we evaluated the gel properties and structures of ι-carrageenan products extracted by Ca(OH) (Ca-IC) and NaOH (Na-IC). The Fourier transform infrared spectroscopy results showed that the structures of Ca-IC and Na-IC did not change remarkably. The results of the thermogravimetric analysis and differential scanning calorimetry showed that Ca-IC had the same thermal stability as Na-IC. The results of the textural analysis showed that Ca-IC had a higher hardness and better chewiness compared to Na-IC. Rheological results indicated that Ca-IC and Na-IC exhibited shear-thinning and non-Newtonian fluid properties, whereas the viscosity of Ca-IC was less than that of Na-IC. In conclusion, this new method of ι-carrageenan extraction using Ca-IC is markedly better and yields higher quality carrageenan than the conventional method of using Na-IC.
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http://dx.doi.org/10.3390/md20070419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322172PMC
June 2022

Pathogenic or Therapeutic: The Mediating Role of Gut Microbiota in Non-Communicable Diseases.

Front Cell Infect Microbiol 2022 7;12:906349. Epub 2022 Jul 7.

Department of Colorectal Surgery, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Noncommunicable diseases (NCDs) lead to 41 million deaths every year and account for 71% of all deaths worldwide. Increasing evidence indicates that gut microbiota disorders are closely linked to the occurrence and development of diseases. The gut microbiota, as a potential transmission medium, could play a key role in the transmission and treatment of diseases. The gut microbiota makes noncommunicable diseases communicable. New methods of the prevention and treatment of these diseases could be further explored through the gut microbiota.
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http://dx.doi.org/10.3389/fcimb.2022.906349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301375PMC
July 2022

Theta-Burst Stimulation Combined With Virtual-Reality Reconsolidation Intervention for Methamphetamine Use Disorder: Study Protocol for a Randomized-Controlled Trial.

Front Psychiatry 2022 5;13:903242. Epub 2022 Jul 5.

Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

Background: Craving associated with drug-related memory is one of the key factors that induce the relapse of methamphetamine (MA). Disruption or modulation of the reconsolidation of drug-related memory may serve as an option for clinical treatment of MA addiction. This protocol proposes to use virtual reality (VR) to retrieve drug-associated memory and then use transcranial magnetic stimulation (TMS) at the neural circuit that encodes the reward value of drug cues to provide a non-invasive intervention during reconsolidation. We aim to evaluate the effectiveness of TMS treatment after VR retrieval on the reduction of cue reactivity and craving of MA.

Methods: This is a randomized, double-blind, sham-controlled, parallel group trial, targeting participants with MA use disorder aged from 18 to 45 years old. Forty-five eligible volunteers in Shanxi Drug Rehabilitation Center will be recruited and be randomly allocated into three parallel groups, receiving either 1) MA-related cues retrieval in VR combined with active TMS (MA VR scene + TBS) or 2) sham TMS (MA VR scene + sham TBS), or 3) neutral cues retrieval in VR combined with active TMS (neutral VR scene + TBS). Two sessions of post-VR-retrieval TBS will be scheduled on two separate days within 1 week. The primary outcome will detect the memory-related activity by the electroencephalography (EEG) reactivity to drug cues in VR scenes. Secondary outcomes are the self-reported MA craving in VR scene, the physiological parameter (cue-induced heart rate) and the scores of psychological questionnaires including anxiety, depression, and mood. All primary and secondary outcomes will be assessed at baseline, 1-week, and 1-month post-intervention. Assessments will be compared between the groups of 1) MA VR scene + TBS, 2) MA VR scene + sham TBS and 3) neutral VR scene + TBS.

Discussion: This will be the first study to examine whether the TMS modulation after VR retrieval can reduce self-reported craving and drug-related cue reactivity. It will promote the understanding of the neural circuit mechanism of the reconsolidation-based intervention and provide an effective treatment for MA use disorder patients.

Clinical Trial Registration: [Chinese Clinical Trial Registry], identifier [ChiCTR1900026902]. Registered on 26 October 2019.
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http://dx.doi.org/10.3389/fpsyt.2022.903242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294395PMC
July 2022

A Modified Trocar and Cannula System With Lateral Micropores for 1-Step Drainage of Suprachoroidal Fluid During Primary Vitrectomy.

JAMA Ophthalmol 2022 Jul 21. Epub 2022 Jul 21.

Department of Ophthalmology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.

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http://dx.doi.org/10.1001/jamaophthalmol.2022.2597DOI Listing
July 2022

Colocalized targeting of TGF-β and PD-L1 by bintrafusp alfa elicits distinct antitumor responses.

J Immunother Cancer 2022 Jul;10(7)

Department of TIP OIO, EMD Serono Research and Development Institute, Billerica, Massachusetts, USA

Background: Bintrafusp alfa (BA) is a bifunctional fusion protein designed for colocalized, simultaneous inhibition of two immunosuppressive pathways, transforming growth factor-β (TGF-β) and programmed death-ligand 1 (PD-L1), within the tumor microenvironment (TME). We hypothesized that targeting PD-L1 to the tumor by BA colocalizes the TGF-β trap (TGF-βRII) to the TME, enabling it to sequester TGF-β in the tumor more effectively than systemic TGF-β blockade, thereby enhancing antitumor activity.

Methods: Multiple technologies were used to characterize the TGF-β trap binding avidity. BA versus combinations of anti-PD-L1 and TGF-β trap or the pan-TGF-β antibody fresolimumab were compared in proliferation and two-way mixed lymphocyte reaction assays. Immunophenotyping of tumor-infiltrating lymphocytes (TILs) and RNA sequencing (RNAseq) analysis assessing stromal and immune landscape following BA or the combination therapy were performed in MC38 tumors. TGF-β and PD-L1 co-expression and their associated gene signatures in MC38 tumors and human lung carcinoma tissue were studied with single-cell RNAseq (scRNAseq) and immunostaining. BA-induced internalization, degradation, and depletion of TGF-β were investigated in vitro.

Results: BA and fresolimumab had comparable intrinsic binding to TGF-β1, but there was an ~80× avidity-based increase in binding affinity with BA. BA inhibited cell proliferation in TGF-β-dependent and PD-L1-expressing cells more potently than TGF-β trap or fresolimumab. Compared with the combination of anti-PD-L1 and TGF-β trap or fresolimumab, BA enhanced T cell activation in vitro and increased TILs in MC38 tumors, which correlated with efficacy. BA induced distinct gene expression in the TME compared with the combination therapy, including upregulation of immune-related gene signatures and reduced activities in TGF-β-regulated pathways, such as epithelial-mesenchymal transition, extracellular matrix deposition, and fibrosis. Regulatory T cells, macrophages, immune cells of myeloid lineage, and fibroblasts were key PD-L1/TGF-β1 co-expressing cells in the TME. scRNAseq analysis suggested BA modulation of the macrophage phenotype, which was confirmed by histological assessment. PD-L1/TGF-β1 co-expression was also seen in human tumors. Finally, BA induced TGF-β1 internalization and degradation in the lysosomes.

Conclusion: BA more effectively blocks TGF-β by targeting TGF-β trap to the tumor via PD-L1 binding. Such colocalized targeting elicits distinct and superior antitumor responses relative to single agent combination therapy.
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http://dx.doi.org/10.1136/jitc-2021-004122DOI Listing
July 2022

Solid-State Janus Nanoprecipitation Enables Amorphous-Like Heat Conduction in Crystalline Mg Sb -Based Thermoelectric Materials.

Adv Sci (Weinh) 2022 Jul 18:e2202594. Epub 2022 Jul 18.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.

Solid-state precipitation can be used to tailor material properties, ranging from ferromagnets and catalysts to mechanical strengthening and energy storage. Thermoelectric properties can be modified by precipitation to enhance phonon scattering while retaining charge-carrier transmission. Here, unconventional Janus-type nanoprecipitates are uncovered in Mg Sb Bi formed by side-by-side Bi- and Ge-rich appendages, in contrast to separate nanoprecipitate formation. These Janus nanoprecipitates result from local comelting of Bi and Ge during sintering, enabling an amorphous-like lattice thermal conductivity. A precipitate size effect on phonon scattering is observed due to the balance between alloy-disorder and nanoprecipitate scattering. The thermoelectric figure-of-merit ZT reaches 0.6 near room temperature and 1.6 at 773 K. The Janus nanoprecipitation can be introduced into other materials and may act as a general property-tailoring mechanism.
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http://dx.doi.org/10.1002/advs.202202594DOI Listing
July 2022

Transcriptional Circuitry of NKX2-1 and SOX1 Defines an Unrecognized Lineage Subtype of Small Cell Lung Cancer.

Am J Respir Crit Care Med 2022 Jul 18. Epub 2022 Jul 18.

Icahn School of Medicine at Mount Sinai, 5925, Department of Medicine, Division of Pulmonary Critical Care and Sleep Medicine, New York, New York, United States;

: The current molecular classification of small cell lung cancer (SCLC) based on expression of four lineage transcription factors still leaves its major subtype SCLC-A as a heterogeneous group, necessitating more precise characterization of lineage subclasses. : To refine the current SCLC classification with epigenomic profiles and to identify features of the re-defined SCLC subtypes. : We performed unsupervised clustering of epigenomic profiles on 25 SCLC cell lines. Functional significance of NKX2-1 was evaluated by cell growth, apoptosis and xenograft using CRISPR-Cas9-mediated deletion. NKX2-1 specific cistromic profiles were determined by ChIP-seq and its functional transcriptional partners were determined using co-immunoprecipitation followed by mass-spectrometry. Rb1Trp53 and Rb1Trp53Nkx2-1 mouse models were engineered to explore the function of Nkx2-1 in SCLC tumorigenesis. Epigenomic landscapes of 6 human SCLC specimens and 20 tumors from 2 mouse models were characterized. : We identified an epigenomic subcluster of the major SCLC-A subtype: SCLC-Aα and SCLC-Aσ. SCLC-Aα was characterized by the presence of a super-enhancer at the NKX2-1 locus, which was observed in human SCLC specimens and a murine SCLC model. We found NKX2-1, a dual lung and neural lineage factor, is uniquely relevant in SCLC-Aα. Additionally, we found maintenance of this neural identity in SCLC-Aα is mediated by collaborative transcriptional activity with another neuronal transcriptional factor SOX1. : We comprehensively describe additional epigenomic heterogeneity of the major SCLC-A subtype, and define SCLC-Aα subtype by the core regulatory circuitry of NKX2-1 and SOX1 super-enhancers and their functional collaborations to maintain neuronal linage state.
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http://dx.doi.org/10.1164/rccm.202110-2358OCDOI Listing
July 2022

TBS combined with virtual-reality reconsolidation intervention for methamphetamine use disorder: A pilot study.

Brain Stimul 2022 Jul 12;15(4):996-998. Epub 2022 Jul 12.

Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.brs.2022.07.001DOI Listing
July 2022

Alcohol Use and Misuse Among Chinese Psychiatrists During the Early COVID-19 Pandemic.

Front Psychiatry 2022 22;13:933814. Epub 2022 Jun 22.

Department of Psychiatry and Behavioral Sciences, Emory University, Atlanta, GA, United States.

Aim: Survey alcohol use and misuse among Chinese psychiatrists during the Coronavirus diseases 2019 (COVID-19) pandemic.

Methods: We conducted a large-scale, nationwide online survey of psychiatrists regarding their alcohol use during the pandemic. The Alcohol Use Disorder Identification Test-Concise (AUDIT-C) was used to assess alcohol use and misuse.

Results: Of 3,815 psychiatrists who completed the survey, alcohol use and misus were 47.5% and 8.2%, respectively, and both were significantly higher in males. The majority (59%) reported no change in alcohol use during the pandemic, one-third (34.5%) reported a decrease, and 6.5% reported an increase. Alcohol misuse was associated with middle-age (OR = 1.418), male sex (OR = 5.089), Northeast China (OR = 1.507), cigarette-smoking (OR = 2.335), insomnia (OR = 1.660), and regular exercise (OR = 1.488). A master's degree (OR = 0.714) and confidence in clinical work (OR = 0.610) were associated with less alcohol misuse. Those who reported a decrease in alcohol use during the pandemic were more likely to be male (OR = 2.011), located in Northeast China (OR = 1.994), and feel confident in their clinical work (OR = 1.624). Increased alcohol use was significantly associated with insomnia (OR = 3.139).

Conclusions: During the COVID-19 pandemic, alcohol use and misuse among Chinese psychiatrists declined. While males were more likely to misuse alcohol, they were also more likely to have reduced their intake. Age, location, and lifestyle factors also predicted alcohol use and misuse. Further examination of specific factors that reduced alcohol use and misuse may help guide public health efforts to sustain the lower rates beyond the pandemic.
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http://dx.doi.org/10.3389/fpsyt.2022.933814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258332PMC
June 2022

Expression Promotes Lung Adenocarcinoma Progression and Is Associated With Poor Prognosis.

Front Oncol 2022 23;12:795971. Epub 2022 Jun 23.

Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Aberrant expression of the gene encoding the Ndc80 kinetochore complex component () reportedly contributes to the progression of several human cancers. However, the functional roles of and their underlying mechanisms in lung adenocarcinoma (LUAD) are largely unknown. The current study aimed to investigate the role of in LUAD tumorigenesis. Here, TCGA, ONCOMINE, the Human Protein Atlas, UALCAN, and the results of our cohort were used to analyze the expression of in LUAD. A Kaplan-Meier analysis and univariate and multivariate Cox regression analyses were performed to estimate the prognostic values of expression in the Cancer Genome Atlas cohort. We studied the effects of expression on proliferation, migration, invasion, and tumor growth using LUAD cell lines. Gene set enrichment analysis (GSEA) was used to analyze the pathways and biological function enrichment of in LUAD. The ssGSEA database was used to analyze the relationship between expression and immune cell infiltration in LUAD. Results revealed elevated expression of in LUAD specimens. Patients overexpressing had poor prognoses relative to those with low expression. Knockdown of suppressed the proliferation, migration, invasion, epithelial-mesenchymal transition, and colony formation of LUAD cells. Moreover, knockdown induced cell cycle arrest at the G0/G1 phase. Gene Ontology and GSEA analyses suggested that may be involved in immunity, proliferation, and apoptosis-related pathways. overexpression was positively correlated with differential immune cell infiltration. In conclusion, expression was associated with the clinical phenotype of LUAD and hence has potential implications in LUAD treatment.
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http://dx.doi.org/10.3389/fonc.2022.795971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259841PMC
June 2022

Depression, Anxiety, Stress, and Their Associations With Quality of Life in a Nationwide Sample of Psychiatrists in China During the COVID-19 Pandemic.

Front Psychol 2022 23;13:881408. Epub 2022 Jun 23.

Department of Psychiatry, Chaohu Hospital of Anhui Medical University, Hefei, China.

Objectives: The COVID-19 pandemic has negatively affected wellbeing. However, the impact on the mental health status of Chinese psychiatrists and their relationship with quality of life (QOL) has not been examined.

Methods: This was a national cross-sectional survey performed between January 11 and March 15, 2021. Demographic and work-related data were collected anonymously using an online questionnaire. Common mental health symptoms and QOL were assessed using the Depression Anxiety Stress Scale-Chinese version and the World Health Organization Quality of Life Schedule-Brief, respectively.

Results: A total of 3,783 psychiatrists completed this questionnaire. The prevalence of depressive, anxious, and stress symptoms were 26.7% (95%CI = 25.3-28.1%), 24.1% (95%CI = 22.8-25.5%), and 11.6% (95%CI = 10-12%), respectively. Moreover, 11.1% of the participants endorsed both depressive and anxious symptoms, and 8% endorsed symptoms in all three domains. Binary logistic regression showed that aged 30-39 years (OR = 1.5, = 0.03), male gender (OR = 1.2, = 0.04), single (OR = 1.44, = 0.01), and having a negative perception of the COVID-19 on healthcare (OR = 2.34, <0.001) were factors associated with higher levels of depressive symptoms. Participants who were divorced and widowed (OR = 1.56, = 0.03), worked more than 4 night shifts/month (OR = 1.33, <0.001) and/or longer working years (OR = 1.71, < 0.001), and had a negative perceived impact of the COVID-19 on healthcare (OR = 2.05, < 0.001) were more inclined to experience anxious symptoms. In addition, psychiatrists with high QOL scores had lower odds of experiencing depressive, anxious, and stress symptoms (all < 0.001). Multivariate analysis showed that the presence of each of the three types of mental health symptoms was independently associated with lower QOL (all < 0.05). In addition, the depression × anxious × stress interaction was significantly correlated with lower QOL ( < 0.05).

Conclusion: Approximately one-fourth of psychiatrists in China experienced depressive and anxious symptoms during the COVID-19 pandemic, and more than one-tenth reported stress. Mental health symptoms were significant contributors to lower QOL. The psychological wellbeing of psychiatrists during the pandemic requires more attention, and interventions are needed to improve the psychological wellbeing and QOL of physicians who care for individuals with mental disorders.
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http://dx.doi.org/10.3389/fpsyg.2022.881408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260312PMC
June 2022

Assessment of quality deviation of pork and salmon due to temperature fluctuations during superchilling.

J Zhejiang Univ Sci B 2022 Jul;23(7):578-586

Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Superchilling is an emerging technology for meat preservation; however, the temperature changes during the process have been commonly ignored. Thus, the effects of temperature fluctuations on meat quality during superchilling are yet to be evaluated. In our study, pork loins and salmon fillets were stored for several days (0, 8, 15, 23, and 30 d) under different temperature fluctuations based on -3.5 ℃ as the target temperature. The results showed that after 15 d of superchilling storage, the values of total volatile basic nitrogen, total viable count, and lipid oxidation were significantly (<0.05) altered in the ±2.0 ℃ fluctuation group compared with the constant temperature group. On the contrary, there was no significant difference in these parameters between the ±1.0 ℃ fluctuation group and the constant temperature group after 30 d of storage. In addition, irregular temperature changes significantly accelerated the modulation of various indicators. In brief, temperature fluctuations and irregular temperature changes accelerated the destruction of muscle structural integrity, increased the water loss, gradually widened the water loss channels, and thereby reduced the edibility by accelerating the spoilage of meat.
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http://dx.doi.org/10.1631/jzus.B2200030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264106PMC
July 2022

Airport noise disturbs foraging behavior of Japanese pipistrelle bats.

Ecol Evol 2022 Jul 12;12(6):e8976. Epub 2022 Jun 12.

Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization Northeast Normal University Changchun China.

The expansion of anthropogenic noise poses an emerging threat to the survival and reproductive success of various organisms. Previous investigations have focused on the detrimental effects of anthropogenic noise on the foraging behavior in some terrestrial and aquatic animals. Nevertheless, the role of airport noise in impairing foraging activities of most wild animals has been neglected. Here, we aimed to assess whether foraging behavior in free-living Japanese pipistrelle bats () can be disturbed by airport noise. We used audio recording to monitor foraging activities of bats at 11 sites around the runway of a municipal airport. We quantified noise level and spectra, aircraft activity, habitat type, nightly temperature, wind speed, and moon phase for each site. The analysis revealed that noise level and aircraft activity were significant negative predictors for the number of bat passes and feeding buzzes around the runway, even after controlling for the effects of other environmental factors. There was no marked spectral overlap between bat echolocation pulses and airport noise in the presence and absence of low-flying aircraft. The spectro-temporal parameters of echolocation vocalizations emitted by bats were dependent on noise level, aircraft activity, and habitat type. These results provide correlative evidence that airport noise can reduce foraging activities of wild pipistrelle bats. Our findings add to the current knowledge of adverse impacts of airport noise on foraging bats in artificial ecosystems and provide a basis for further research on the mechanisms behind noise pollution near airports.
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http://dx.doi.org/10.1002/ece3.8976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189338PMC
July 2022

A General Map of Transcriptional Expression of Virulence, Metabolism, and Biofilm Formation Adaptive Changes of When Exposed to Different Antimicrobials.

Front Microbiol 2022 17;13:825041. Epub 2022 Jun 17.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Biofilm formation of is the major cause of implant-associated infections (IAIs). Antimicrobial treatment is one of the most effective therapeutic options for infections. However, it can also lead to adaptive transcriptomic changes due to extreme selective pressure, which may increase the risk of antimicrobial resistance. To study the transcriptional changes in upon exposure to antimicrobial agents, we obtained expression profiles of treated with six antimicrobials (flucloxacillin, vancomycin, ciprofloxacin, clindamycin, erythromycin, and linezolid, = 6 for each group). We also included an untreated control group ( = 8) downloaded from the Gene Expression Omnibus (GEO) database (GSE70043, GSE56100) for integrated bioinformatic analyses. We identified 82 (44 up, 38 down) and 53 (17 up, 36 down) differentially expressed genes (DEGs) in logarithmic and stationary phases, respectively. When exposed to different antimicrobial agents, we found that manganese import system genes and immune response gene (immunoglobulin G-binding protein Sbi) were upregulated in at all stages. During the logarithmic phase, we observed adaptive transcriptomic changes in mainly in the stability of protein synthesis, adhesion, and biofilm formation. In the stationary phase, we observed a downregulation in genes related to amino biosynthesis, ATP synthesis, and DNA replication. We verified these results by qPCR. Importantly, these results could help our understanding of the molecular mechanisms underlying the proliferation and antimicrobial resistance of .
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http://dx.doi.org/10.3389/fmicb.2022.825041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247510PMC
June 2022

Genomic Landscape of Metastatic Lymph Nodes and Primary Tumors in Non-Small-Cell Lung Cancer.

Pathol Oncol Res 2022 16;28:1610020. Epub 2022 Jun 16.

The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

To investigate the genetic mutation characteristics of non-small cell lung cancers (NSCLC) with and without lymph node metastasis. Primary lesions and metastatic lymph node lesions of 36 Chinese NSCLC patients were tested for somatic mutations, tumor mutation burden, phylogenetic and clonal evolutional analysis using a 1021-gene panel by next-generation sequencing (NGS) with an average sequencing depth of 671X. In this study, eighteen patients with lung adenocarcinoma (LUAD) and 18 with lung squamous cell carcinoma (LUSC) were included. Different groups had distinct characteristics of gene mutations. CTNNB1 gene mutations were only present in Nome_LC LUAD patients ( < 0.05). ARID1A mutation was however the only gene with significant alterations ( < 0.05) in Nome_LC in LUSC. Phylogenetic trees of mutated genes were also constructed. Linear and parallel evolutions of metastatic lymph nodes were observed both in LUAD and LUSC. LUSC exhibited more genetic mutations than LUAD. Intriguingly, there was significant difference in gene mutations between Meta_LC and Nome_LC. CTNNB1 gene alteration was the key mutation in LUAD that seems to promote proliferation of the tumor and then determine T stage. On the other hand, proliferation of the tumor was characterized by ARID1A missense mutation in LUSC, thus influencing the T stage as well. Lymph node metastasis could display both linear and parallel evolutionary characteristics in NSCLC. Different metastatic lymph nodes might have exactly the same or different mutated genes, underlining the heterogeneous genomic characteristics of these cancer types.
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http://dx.doi.org/10.3389/pore.2022.1610020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243222PMC
July 2022

Workplace Violence and Turnover Intention Among Psychiatrists in a National Sample in China: The Mediating Effects of Mental Health.

Front Psychiatry 2022 15;13:855584. Epub 2022 Jun 15.

Vanke School of Public Health, Tsinghua University, Beijing, China.

Background: Workplace violence (WPV) in healthcare has received much attention worldwide. However, scarce data are available on its impact on turnover intention among psychiatrists, and the possible mechanisms between WPV and turnover intention have not been explored in China.

Methods: A cross-sectional survey was conducted among psychiatrists in 41 tertiary psychiatric hospitals from 29 provinces and autonomous regions in China. A stress-strain-outcome (SSO) model was adopted to examine the effects of WPV on mental health and turnover intention. The association and mediation by burnout and stress were examined by multivariate logistic regression (MLR) and generalized structure equation modeling (GSEM).

Results: We invited 6,986 psychiatrists to participate, and 4,520 completed the survey (64.7% response rate). The prevalence of verbal and physical violence against psychiatrist in China was 78.0 and 30.7%, respectively. MLR analysis showed that psychiatrists who experienced verbal violence (OR = 1.15, 95% CI = 1.10-1.21) and physical violence (OR = 1.15, 95% CI = 1.07-1.24) were more likely to report turnover intention. GSEM analysis showed that burnout (β = 4.00, < 0.001) and stress (β = 1.15, < 0.001) mediated the association between verbal violence and turnover intention; similarly, burnout (β = 4.92, < 0.001) and stress (β = 1.80, < 0.001) also mediated the association between physical violence and turnover intention.

Conclusions: Experience of WPV is a significant contributor to turnover intention among psychiatrists. Mental health status, such as burnout and stress level significantly mediated the association. Policy makers and hospital administrators need to be aware of this association. Action is needed to promote mental health among the psychiatrists to improve morale and workforce sustainability.
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http://dx.doi.org/10.3389/fpsyt.2022.855584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240432PMC
June 2022

Performance optimization strategies of halide perovskite-based mechanical energy harvesters.

Nanoscale Horiz 2022 Jul 1. Epub 2022 Jul 1.

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.

Halide perovskites, possessing unique electronic and photovoltaic properties, have been intensively investigated over the past decade. The excellent polarization, piezoelectricity, dielectricity and photoelectricity of halide perovskites provide new opportunities for the applications of mechanical energy harvesting. Although various studies have been conducted to develop halide perovskite-based triboelectric and piezoelectric nanogenerators, strategies for their electrical performance optimization are rarely mentioned. In this review, we systematically introduce the recent research progress of halide perovskite-based mechanical energy harvesters and summarize the different optimization strategies for improving both the piezoelectric and triboelectric output of the devices, bringing some inspiration to guide future material and structure design for halide perovskite-based energy devices. A summary of the current challenges and future perspectives is also presented, offering some possible directions for development in this emerging field.
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http://dx.doi.org/10.1039/d2nh00229aDOI Listing
July 2022

Editorial: Revisiting Cross-Cultural Comparison on International Business: Challenges and Opportunities.

Front Psychol 2022 13;13:941217. Epub 2022 Jun 13.

School of Public Health and The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

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http://dx.doi.org/10.3389/fpsyg.2022.941217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235566PMC
June 2022

Effect of distributed Bragg reflectors on photoluminescence properties of CHNHPbI film.

Sci Rep 2022 Jun 29;12(1):10934. Epub 2022 Jun 29.

School of Physics, Dalian University of Technology, Dalian, 116024, People's Republic of China.

The nanoporous (NP) GaN distributed Bragg reflector (DBR) was prepared by using electrochemical etching. Then the NP-GaN DBR was pretreated by using ozone treatment. Atomic force microscopy and X-ray diffraction (XRD) were used to investigate the influence of ozone treatment on the structure of the substrates. The hybrid organic-inorganic CHNHPbI perovskite films were grown on the NP-GaN DBR and reference substrates by using a one-step solution method. The XRD and field emission scanning electron microscopy test results indicate the high quality of the prepared CHNHPbI perovskite films. The photoluminescence intensity of the prepared CHNHPbI perovskite film on the NP-GaN DBR substrate is ~ 3.5 times higher than that of the film on the reference substrate, with a 3.6 nm spectral blue-shift. The enhancement should be contributable to amplify spontaneous emission by resonant cavity, while the blue-shift could be contributable to stoichiometric difference of the films on different substrates.
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http://dx.doi.org/10.1038/s41598-022-14991-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243019PMC
June 2022

Turnover Intention and Its Associated Factors Among Psychiatrists in 41 Tertiary Hospitals in China During the COVID-19 Pandemic.

Front Psychol 2022 10;13:899358. Epub 2022 Jun 10.

Department of Psychiatry and Behavioral Sciences, Emory University, Atlanta, GA, United States.

Background: Turnover intention, an employee's intention to voluntarily leave their jobs, affects workforce sustainability. However, scarce data are available about turnover intention and its associated factors among psychiatrists in China, especially during the COVID-19 pandemic. The current research was designed to address this gap.

Methods: An anonymous, nationwide online survey was disseminated to psychiatrists in 41 psychiatric hospitals in China. We collected demographic data, job-related information (duration of employment, history of participation in the frontline work against COVID-19, job satisfaction), and participants' turnover intention in the next 12 months.

Results: In total, 3,973 psychiatrists completed the survey. The sample was predominantly female (58.8%) and married (79.5%), and nearly three-fourths (73.5%) had children. More than one-third (35.6%) had a master's or doctoral degree in addition to their medical degree. The overall level of job satisfaction was moderate. The rate of turnover intention was 22.0% and was comparable in males and females (22.9% in males and 21.3% in females, respectively). Psychiatrists who had participated in the frontline work of COVID-19 were more likely to report an intention to leave their current job. Multiple regression analyses suggested that turnover intention was significantly associated with having insomnia, longer working hours, and more working days per week.

Conclusion: During the pandemic period, approximately one-fifth of psychiatrists in China reported turnover intention. Factors associated with turnover intention included high job-related burdens, low job satisfaction, participation in the frontline work against COVID-19, and insomnia. To improve psychiatric workforce sustainability, policymakers and hospital administrators need to be aware of this potential challenge and address the concerns of psychiatrists in China.
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http://dx.doi.org/10.3389/fpsyg.2022.899358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226451PMC
June 2022

Comparative Analysis of Gut Microbial Composition and Functions in Przewalski's Gazelle () From Various Habitats.

Front Microbiol 2022 8;13:913358. Epub 2022 Jun 8.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Gut microbiota of mammals participates in host nutrient metabolism and plays an important role in host adaptation to the environment. Herein, to understand the relationship between environment differences and the composition and abundance of the gut microbiota of Przewalski's gazelle () in almost all its habitats, high throughput sequencing of the 16S rRNA gene was used to compared the characteristics of the gut microbiota based on total 120 fecal samples. The results showed that Przewalski's gazelle exhibited different characteristics of microbiota diversity in different habitats. The Jiangxigou Rescue Station (JX), Nongchang (NC), and Ganzihe and Haergai townships (GH) groups had a relatively high microbiota diversity, while the Niaodao scenic area (ND) group had the lowest diversity. This finding seemed to follow a similar pattern of change in the population of Przewalski's gazelle. and were the phyla with significant differences, especially between the Wayu township (WY) and the other groups. The difference in the microbiota mainly included the , , and and was enriched in the ND, WY, and other regions. We speculated that the difference in the gut microbiota was due to a difference in environmental characteristics, particularly the food resources that the host can obtain. We speculated that a similar microbiome has important functions for species survival and represents the evolutionary commonality of Przewalski's gazelle, while a different microbiome plays an important role in the adaptation of Przewalski's gazelle to a different environment. The results of this study illustrate how the same species adapts to different environments from the perspective of gut microbiota plasticity and therefore are of great significance for the protection and restoration of the population of this species.
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http://dx.doi.org/10.3389/fmicb.2022.913358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213746PMC
June 2022

Sappanwood-derived polyphenolic antidote of amyloidal toxins achieved detoxification via inhibition/reversion of amyloidal fibrillation.

Int J Biol Macromol 2022 Aug 23;214:446-458. Epub 2022 Jun 23.

Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA.

The formidable virulence of methicillin-resistant staphylococcus aureus (MRSA) have thrown great challenges to biomedicine, which mainly derives from their autocrine phenol-soluble modulins (PSMs) toxins, especially the most toxic member termed phenol-soluble modulins α3 (PSMα3). PSMα3 cytotoxicity is attributed to its amyloidal fibrillation and subsequent formation of cross-α sheet fibrils. Inspired by the multiple biological activity of Sappanwood, herein, we adopted brazilin, a natural polyphenolic compound originated from Caesalpinia sappan, as a potential antidote of PSMα3 toxins, and attempted to prove that the regulation of PSMα3 fibrillation was an effective alexipharmic way for MRSA infections. In vitro results revealed that brazilin suppressed PSMα3 fibrillation and disassembled preformed amyloidal fibrils in a dose-dependent manner, in which molar ratio (brazilin: PSMα3) of efficient inhibition and disassembly were both 1:1. These desired regulations dominated by brazilin benefited from its bonding to core fibrils-forming residues of PSMα3 monomers urged by hydrogen bonding and pi-pi stacking, and such binding modes facilitated brazilin-mediated inhibition or disruption of interactions between neighboring PSMα3 monomers. In this context, these inhibited and disassembled PSMα3 assemblies could not easily insert into cell membrane and subsequent penetration, and thus alleviating the membrane disruption, cytoplasmic leakage, and reactive oxygen species (ROS) generation in normal cells. As such, brazilin dramatically decreased the cytotoxicity borne by toxic PSMα3 fibrils. In addition, in vivo experiments affirmed that brazilin relieved the toxicity of PSMα3 toxins and thus promoted the skin wound healing of mice. This study provides a new antidote of PSMα3 toxins, and also confirms the feasibility of the assembly-regulation strategy in development of antidotes against supramolecular fibrillation-dependent toxins.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.141DOI Listing
August 2022

Percolated Sulfide in Salt-Concentrated Polymer Matrices Extricating High-Voltage All-Solid-State Lithium-metal Batteries.

Adv Sci (Weinh) 2022 Jun 24:e2202474. Epub 2022 Jun 24.

Qingdao Industrial Energy Storage Research Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, P. R. China.

All-solid-state lithium-metal batteries (ASLMBs) are considered to be remarkably promising energy storage devices owing to their high safety and energy density. However, the limitations of current solid electrolytes in oxidation stability and ion transport properties have emerged as fundamental barriers in practical applications. Herein, a novel solid electrolyte is presented by in situ polymerization of salt-concentrated poly(ethylene glycol) diglycidyl ether (PEGDE) implanted with a three-dimensional porous L GeP S skeleton to mitigate these issues. The poly(PEGDE) endows more oxygen atoms to coordinate with Li , significantly lowering its highest occupied molecular orbital level. As a consequence, the electro-oxidation resistance of poly(PEGDE) exceeds 4.7 V versus Li /Li. Simultaneously, the three-dimensonal porous L GeP S skeleton provides a percolated pathway for rapid Li migration, ensuring a sufficient ionic conductivity of 7.7 × 10 S cm at room temperature. As the bottlenecks are well solved, 4.5 V LiNi Mn Co O -based ASLMBs present fantastic cycle performance over 200 cycles with an average Coulombic efficiency exceeding 99.6% at room temperature.
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http://dx.doi.org/10.1002/advs.202202474DOI Listing
June 2022
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