Publications by authors named "Feng Huang"

715 Publications

Inhibition of HMGB1 alleviates myocardial ischemia/reperfusion injury in diabetic mice via suppressing autophagy.

Microvasc Res 2021 Jun 10:104204. Epub 2021 Jun 10.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Guangxi Key Laboratory of Precision Medicine in Cardio-Cerebrovascular Diseases Control and Prevention, Guangxi Clinical Research Center for Cardio-Cerebrovascular Diseases, Nanning, Guangxi, China. Electronic address:

Background: Diabetes aggravates myocardial ischemia/reperfusion (I/R) injury (MI/RI). The association between high mobility group box 1 protein (HMGB1) and autophagy in diabetic MI/RI remains unknown. Therefore, we investigated whether inhibiting HMGB1 can regulate autophagy in diabetic mice (DM) after I/R injury.

Methods: I/R models of C57BL/KsJ mice and db/db mice were established. Histological changes, infarct size (IS), HMGB1 protein, and autophagy-related proteins were detected after 24 hours of reperfusion. In DM treatment groups, anti-HMGB1 antibody (H-Ig) was injected via tail vein after reperfusion for 15 minutes, and the above-mentioned experimental methods were performed at the end of reperfusion.

Results: Compared with the I/R group, the pathological myocardial damage and IS were significantly increased in the I/R (DM) group. Additionally, the levels of HMGB1, Beclin1, and LC3II/LC3I ratio were remarkably higher in the I/R (DM) group than those in the I/R group, while p62 level was lower. In the H-Ig (DM) group, injection of H-Ig significantly reduced the IS, as well as alleviated pathological myocardial damage. Moreover, Beclin1, LC3II/LC3I ratio, and p62 levels were notably reversed after this treatment.

Conclusions: I/R-induced myocardium was aggravated by diabetes, which may be related to increased release of HMGB1 and activated autophagy. Inhibition of HMGB1 alleviates diabetic MIRI which was associated with reduced autophagy.
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http://dx.doi.org/10.1016/j.mvr.2021.104204DOI Listing
June 2021

Do Pit vipers Assess Their Venom? Defensive Tactics of Deinagkistrodon acutus Shift with Changed Venom Reserve.

Toxicon 2021 Jun 6. Epub 2021 Jun 6.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China. Electronic address:

To maximize survival probability, animals must assess predation risks and adopt flexible defensive strategies based on specific conditions. Pit vipers utilize venom for predation and self-defense, and venom status significantly influences its effectiveness. Thus, pit vipers may evaluate their venom reserve and adopt corresponding defensive tactics. Twenty-three sharp-snouted pit vipers (Deinagkistrodon acutus) were grouped by different venom status and were subjected to eight behavior trials. Subjects' defensive behaviors were recorded and analyzed. Results showed that the normal venom group displayed stable responses across the trials. The low venom group showed fewer strikes and more fleeing behaviors at the end of experiments. After given prolonged intervals for replenishing the venom, significant increases of strike behaviors were observed in the replenishing venom group. These results demonstrated the capability of adopting flexible defensive tactics based on varied venom reserve and provided new evidence for venom-status-recognition.
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http://dx.doi.org/10.1016/j.toxicon.2021.06.003DOI Listing
June 2021

A new discovery of STAT4 single nucleotide polymorphisms associated with hepatocellular carcinoma risk in Chinese Han population: a case-control study.

Biosci Rep 2021 Jun 8. Epub 2021 Jun 8.

Xi'an Medical University, Xi'an, China.

Background: Hepatocellular carcinoma (HCC) is a common fatal malignant tumor worldwide. STAT4 is HCC susceptibility gene identified by genome-wide association study. The purpose of this study was to determine the association between four candidate single nucleotide polymorphisms (SNPs) in STAT4 genes and HCC risk in Chinese Han population.

Methods: A case-control study was conducted to assess the association between STAT4 SNPs and HCC risk in 1011 Chinese Han population. Agena MassARRAY was used to genotype SNPs. The association between SNPs and HCC susceptibility under different genetic models was evaluated by logistic regression analysis. Multifactorial dimension reduction (MDR) analyzed the interaction of 'SNP-SNP' in HCC risk. The difference of clinical characteristics between different genotypes was completed by ANOVA.

Results: The results showed that STAT4 rs11889341 was significantly associated with HCC risk under multiple genetic models (homozygote: OR = 0.60, p = 0.033; recessive: OR = 0.63, p = 0.028; log-additive: OR = 0.83, p = 0.032). The results of subgroup analysis showed that STAT4 rs11889341 is significantly associated with HCC risk with participants who were > 55 years, male or smoking. Both STAT4 rs7574865 and rs10174238 were significantly associated with HCC risk among participants who were > 55 years old, smoking or drinking. STAT4 haplotype (Trs11889341Trs7574865) could reduce the risk of HCC. In addition, rs11889341 and rs7574865 were significantly associated with the level of serum ferritin.

Conclusion: STAT4 rs11889341, rs7574865 or rs10174238 is potentially associated with HCC risk in Chinese Han population. In particular, rs11889341 showed outstanding association with HCC risk.
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http://dx.doi.org/10.1042/BSR20210124DOI Listing
June 2021

Comparative Analysis of Clinical and Imaging Features of Osteomalacia and Spondyloarthritis.

Front Med (Lausanne) 2021 20;8:680598. Epub 2021 May 20.

Department of Rheumatology and Immunology, The First Medical Center of PLA General Hospital, Beijing, China.

To compare the clinical and radiological characteristics of osteomalacia and spondyloarthritis/ankylosing spondylitis (SpA/AS) in order to provide a basis for differential diagnosis. We carried out a retrospective analysis of patients who were diagnosed with osteomalacia at the First Medical Center of 301 Hospital (Beijing, China) from January 2012 to January 2019. The clinical and radiological data of all patients were collected; at the same time, we selected age- and gender-matched patients with SpA/AS for comparison. We enrolled a total of 76 patients, 38 with osteomalacia, and 38 with SpA/AS. The mean ages of the two groups were, respectively 44.62 ± 14.90 years and 44.85 ± 9.76 years ( > 0.05). Of patients with osteomalacia, 65.79% ( = 25) had previously been misdiagnosed with SpA/AS. In the osteomalacia and SpA/AS groups, there were, respectively 31 and 33 patients with low back pain, 22 and 13 patients with peripheral arthralgia, and 13 and 3 patients with heel pain. Alkaline phosphatase (ALP) level was significantly higher in the osteomalacia than in the SpA/AS group ( < 0.05). Serum phosphorus levels, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) levels, and bone mineral density (BMD) were significantly lower in the osteomalacia group than the SpA/AS group ( < 0.05). Twenty-five patients in the osteomalacia group underwent sacroiliac-joint magnetic-resonance imaging (SIJ-MRI); abnormalities were found in 10 of these patients, seven of whom met the definition for positive SIJ-MRI according to 2009 Assessment of SpondyloArthritis international Society (ASAS) criteria. All seven presented with bilateral sacral involvement. Logistic-regression analysis found that the odds ratio (OR) for bone erosion score was 0.551; the higher this score, the lower the possibility of osteomalacia. Clinical and radiological presentations of patients with osteomalacia could highly simulate those of patients with spondyloarthritis; identifying the differences between these two diseases could effectively decrease the misdiagnosis rate.
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http://dx.doi.org/10.3389/fmed.2021.680598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172786PMC
May 2021

Exosomally derived Y RNA fragment alleviates hypertrophic cardiomyopathy in transgenic mice.

Mol Ther Nucleic Acids 2021 Jun 20;24:951-960. Epub 2021 Apr 20.

Smidt Heart Institute, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.

Cardiosphere-derived cell exosomes (CDC) and YF1, a CDC-derived non-coding RNA, elicit therapeutic bioactivity in models of myocardial infarction and hypertensive hypertrophy. Here we tested the hypothesis that YF1, a 56-nucleotide Y RNA fragment, could alleviate cardiomyocyte hypertrophy, inflammation, and fibrosis associated with hypertrophic cardiomyopathy (HCM) in transgenic mice harboring a clinically relevant mutation in cardiac troponin I (cTnI). By quantitative PCR, YF1 was detectable in bone marrow, spleen, liver, and heart 30 min after intravenous (i.v.) infusion. For efficacy studies, mice were randomly allocated to receive i.v. YF1 or vehicle, monitored for ambulatory and cardiac function, and sacrificed at 4 weeks. YF1 (but not vehicle) improved ambulation and reduced cardiac hypertrophy and fibrosis. In parallel, peripheral mobilization of neutrophils and proinflammatory monocytes was decreased, and fewer macrophages infiltrated the heart. RNA-sequencing of macrophages revealed that YF1 confers substantive and broad changes in gene expression, modulating pathways associated with immunological disease and inflammatory responses. Together, these data demonstrate that YF1 can reverse hypertrophic and fibrotic signaling pathways associated with HCM, while improving function, raising the prospect that YF1 may be a viable novel therapeutic candidate for HCM.
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http://dx.doi.org/10.1016/j.omtn.2021.04.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141670PMC
June 2021

Active site architecture of an acetyl xylan esterase indicates a novel cold adaptation strategy.

J Biol Chem 2021 May 28:100841. Epub 2021 May 28.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. Electronic address:

SGNH-type acetyl xylan esterases (AcXEs) play important roles in marine and terrestrial xylan degradation, which are necessary for removing acetyl side groups from xylan. However, only a few cold-adapted AcXEs have been reported, and the underlying mechanisms for their cold adaptation are still unknown due to the lack of structural information. Here, a cold-adapted AcXE, AlAXEase, from the Arctic marine bacterium Arcticibacterium luteifluviistationis SM1504 was characterized. AlAXEase could deacetylate xylooligosaccharides and xylan, which, together with its homologs, indicates a novel SGNH-type carbohydrate esterase family. AlAXEase showed the highest activity at 30°C and retained over 70% activity at 0°C, but had unusual thermostability with a T value of 56°C. To explain the cold adaption mechanism of AlAXEase, we next solved its crystal structure. AlAXEase has similar noncovalent stabilizing interactions to its mesophilic counterpart at the monomer level and forms stable tetramers in solution, which may explain its high thermostability. However, a long loop containing the catalytic residues Asp200 and His203 in AlAXEase was found to be flexible due to the reduced stabilizing hydrophobic interactions and increased destabilizing asparagine and lysine residues, leading to a highly flexible active site. Structural and enzyme kinetic analyses combined with molecular dynamics simulations at different temperatures revealed that the flexible catalytic loop contributes to the cold adaptation of AlAXEase by modulating the distance between the catalytic His203 in this loop and the nucleophilic Ser32. This study reveals a new cold adaption strategy adopted by the thermostable AlAXEase, shedding light on the cold adaption mechanisms of AcXEs.
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http://dx.doi.org/10.1016/j.jbc.2021.100841DOI Listing
May 2021

Determination of the optimal ecological water conveyance volume for vegetation restoration in an arid inland river basin, northwestern China.

Sci Total Environ 2021 Sep 15;788:147775. Epub 2021 May 15.

Hubei Key Laboratory of Yangtze River Basin Environmental Aquatic Science, School of Environmental Studies & State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China.

Overexploitation of water resources has led to severe ecological degradation and even desertification in some arid inland river basins, northwestern China. To alleviate or restore the degraded vegetation ecosystem, ecological water conveyance (EWC) has become an important and effective measure. Scientific assessment of the impact of EWC on vegetation restoration and determination of the corresponding optimal EWC volume (EWCV) are important to formulate rational ecological water management. In this study, long time series normalized difference vegetation index (NDVI) was used to extract the restored vegetation area in Qingtu Lake area, a terminal lake in inland Shiyang River basin, northwestern China. The relationship between restored vegetation coverage and EWC was explored to determine the optimal EWCV. The restored vegetation area (RVA) increased dramatically in the first five years and became stable from 2016. The time lag of the response of RVA increase to EWC was about 2 years. A bell-shaped function between RVA and groundwater depth was obtained based on the results from Unmanned Aerial Vehicle (UAV) and micro terrain of the lake area. Based on the fitted function, five groundwater depth thresholds were obtained. The optimal groundwater depth in the hydrometric station was 2.91 ± 0.09 m for the maximal RVA (17.08 ± 3.25 km). A polynomial function between the yearly EWCV and groundwater depth was developed and the EWCV thresholds corresponding to the groundwater depth thresholds were estimated. The optimal EWCV into Qingtu Lake was 2224.4 × 10 m for the maximal RVA. The correspondingly optimal EWCV from Hongyashan Reservoir was 3271.4 × 10 m. The spatial distribution patterns of remotely sensed water surface and NDVI suggested that expanding the water-receiving area of conveyed water was useful to improve the vegetation growth. This study provides a reference for assessing the impact of EWC on vegetation restoration and determining the correspondingly optimal EWCV in arid inland river basins.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147775DOI Listing
September 2021

A New Nomogram for Predicting Overall Survival and Assisting Postoperative Adjuvant Treatment Decision-Making in Stage II Oral Tongue Squamous Cell Carcinoma: A Surveillance, Epidemiology and End Results (SEER) Database Analysis.

J Oral Maxillofac Surg 2021 Apr 17. Epub 2021 Apr 17.

Attending, Department of Stomatology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address:

Purpose: The survival benefit of postoperative adjuvant treatment (POAT) for stage II oral tongue squamous cell carcinoma (OTSCC) remains controversial. This large SEER-based study aims to establish a prognostic nomogram to visualize the overall survival of these patients and to aid in POAT decision making.

Patients And Methods: The cut-off points of age at diagnosis and examined lymph node number (ELN) were determined using the population-based data from the SEER database. Univariate and multivariate Cox hazards regression models were utilized to identify prognostic factors that were integrated into the establishment of the prognostic nomogram. Patients with stage II OTSCC were then stratified into 3 cohorts based on this nomogram. The survival benefit of POAT was evaluated in these cohorts.

Results: Age at diagnosis (with cutoff points of 50 and 75 years) and ELN (with cutoff points of 0 and 22) was significantly associated with the survival outcomes in patients with stage II OTSCC. After the multivariate analysis, 4 factors, including age at diagnosis, sex, ELN, and differentiation grade, were identified as independent prognostic factors. Additionally, a prognostic nomogram with these factors was constructed to predict overall survival and to stratify these patients. Only patients in the high-risk cohort could significantly benefit from postoperative adjuvant treatment.

Conclusions: This prognostic nomogram could accurately predict the overall survival of stage II OTSCC patients after curative surgery. Notably, this model could also assist the decision-making of postoperative adjuvant treatment for patients with stage II OTSCC.
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http://dx.doi.org/10.1016/j.joms.2021.04.010DOI Listing
April 2021

The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι.

Proc Natl Acad Sci U S A 2021 06;118(23)

Institute of Human Virology, Key Laboratory of Tropical Disease Control of Ministry of Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, Guangdong, China;

COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic and has claimed over 2 million lives worldwide. Although the genetic sequences of SARS-CoV and SARS-CoV-2 have high homology, the clinical and pathological characteristics of COVID-19 differ significantly from those of SARS. How and whether SARS-CoV-2 evades (cellular) immune surveillance requires further elucidation. In this study, we show that SARS-CoV-2 infection leads to major histocompability complex class Ι (MHC-Ι) down-regulation both in vitro and in vivo. The viral protein encoded by open reading frame 8 (ORF8) of SARS-CoV-2, which shares the least homology with SARS-CoV among all viral proteins, directly interacts with MHC-Ι molecules and mediates their down-regulation. In ORF8-expressing cells, MHC-Ι molecules are selectively targeted for lysosomal degradation via autophagy. Thus, SARS-CoV-2-infected cells are much less sensitive to lysis by cytotoxic T lymphocytes. Because ORF8 protein impairs the antigen presentation system, inhibition of ORF8 could be a strategy to improve immune surveillance.
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http://dx.doi.org/10.1073/pnas.2024202118DOI Listing
June 2021

The interferon-stimulated exosomal hACE2 potently inhibits SARS-CoV-2 replication through competitively blocking the virus entry.

Signal Transduct Target Ther 2021 05 12;6(1):189. Epub 2021 May 12.

Institute of Human Virology, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China.

Since the outbreak of coronavirus disease 2019 (COVID-19), it has become a global pandemic. The spike (S) protein of etiologic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specifically recognizes human angiotensin-converting enzyme 2 (hACE2) as its receptor, which is recently identified as an interferon (IFN)-stimulated gene. Here, we find that hACE2 exists on the surface of exosomes released by different cell types, and the expression of exosomal hACE2 is increased by IFNα/β treatment. In particular, exosomal hACE2 can specifically block the cell entry of SARS-CoV-2, subsequently inhibit the replication of SARS-CoV-2 in vitro and ex vivo. Our findings have indicated that IFN is able to upregulate a viral receptor on the exosomes which competitively block the virus entry, exhibiting a potential antiviral strategy.
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http://dx.doi.org/10.1038/s41392-021-00604-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113286PMC
May 2021

Evaluation of volatile flavor compounds in bacon made by different pig breeds during storage time.

Food Chem 2021 Apr 9;357:129765. Epub 2021 Apr 9.

University of Liège, Gembloux Agro-Bio Tech, Unit of Food Science and Formulation, Passage des Déportés 2, Gembloux B-5030, Belgium.

The oxidation and volatile constituents of bacon (which made of white pig (Duroc × Landrace × Yorkshire) and black pig (Beijing Black × Yorkshire)) during refrigerated storage were analyzed by thiobarbituric acid reactive substances (TBARS), total thiol groups, gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupling with ion mobility spectrometry (GC-IMS). The TBARS value of tested samples increased while the total thiol groups decreased during 60 days storage (P < 0.05). Volatile fingerprint results were obtained by GC-MS and GC-IMS detector, the classes and contents of volatile compounds detected in WP bacon were much abundant than BP bacon. Higher phenols and acids concentrations were observed in WP bacon, while alcohols, ketones, and nitrogen-containing compounds were more determined in BP bacon. Besides, WP bacon was more notably affected by storage rather than BP bacon, and the difference of raw material is the main reason for the flavor in WP and BP bacon comparing with the storage process.
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http://dx.doi.org/10.1016/j.foodchem.2021.129765DOI Listing
April 2021

Chinese and global burdens of gastric cancer from 1990 to 2019.

Cancer Med 2021 May 1;10(10):3461-3473. Epub 2021 May 1.

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, P. R. China.

Background: Gastric cancer is a common cancer in China. This project investigated the disease burden of gastric cancer from 1990 to 2019 in China and globally.

Methods: The global age-standardized rates (ASRs) were extracted from the Global Burden of Disease. Moreover, the estimated annual percentage changes (eAPCs) in the ASRs of incidence (ASIR), mortality (ASMR), and disability-adjusted life-years (DALYs) were calculated to determine the trends by countries and regions.

Results: In China, the ASIR declined from 37.56 to 30.64 per 100,000 and the ASMR declined from 37.73 to 21.72 per 100,000. The global ASIR decreased from 22.44 to 15.59 and the ASMR declined from 20.48 to 11.88 per 100,000 persons from 1990 to 2019. The ASIR was the lowest in Malawi (3.28 per 100,000) and the highest in Mongolia (43.7 per 100,000), whereas the ASMR was the lowest in the United States of America (3.40 per 100,000) and the highest in Mongolia (40.04 per 100,000) in 2019. The incidence of early-onset gastric cancer increased in China. The DALYs attributed to gastric cancer presented a slight decrease during the period. China had a higher mortality/incidence ratio (0.845) and 5-year prevalence (27.6/100,000) than most developed countries.

Conclusion: China presented a steady decline in the incidence and mortality rates for gastric cancer. The global ASIR, ASMR, and DALYs showed a slight rise decrease. Different patterns of gastric cancer rates and temporal trends have been identified in different geographical regions, indicating that specific strategies are needed to prevent the increase in some countries.
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http://dx.doi.org/10.1002/cam4.3892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124120PMC
May 2021

Laser Tuning in Layered -BN Crystals.

J Phys Chem Lett 2021 Apr 13;12(15):3795-3801. Epub 2021 Apr 13.

State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials, Sun Yat-sen University, Guangzhou 510275, China.

Integrated optics shows great potential in the current optical communication systems, sensor technology, optical computers, and other fields. Tunable laser technology within a certain range is the key to achieving on-chip optical integration; to realize which, Raman scattering is a competitive method that can effectively transfer incident laser energy to optical phonons due to the photon-phonon interaction. Here, we take hexagonal boron nitride as the energy conversion medium, and based on the angle-resolved polarized Raman spectroscopy, it is found that when laser polarization vector ⊥ axis, the spectrum obtains maximal scattering across the cross section and a minimal depolarization ratio. At room temperature, -BN obtains an output signal with a wavelength of 522.8 nm and a full-width at half-maximum of 0.24 nm under the excitation of 488 nm pump laser, and the depolarization ratio is 0.09 (theoretically, it is 0, and this difference is due to experimental errors). And then, within the temperature range of 80∼420 K, the scattered light wavelength shows a high-precision shift of 0.006 nm/25 K, indicating that continuous wavelength tuning has been successfully achieved in -BN.
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http://dx.doi.org/10.1021/acs.jpclett.1c00958DOI Listing
April 2021

Tunable ultrasharp terahertz plasma edge in a lightly doped narrow-gap semiconductor.

Opt Express 2021 Mar;29(6):9261-9268

Plasma edges in metals typically occur in the visible range, producing characteristic colors of metals. In a lightly doped semiconductor, the plasma edge can occur in the terahertz (THz) frequency range. Due to low scattering rates and variable electron densities in semiconductors, such THz plasma edges can be extremely sharp and greatly tunable. Here, we show that an ultrasharp THz plasma edge exists in a lightly n-doped InSb crystal with a record-high transmittance slope of 80 dB/THz. The frequency at which this sharp edge happens can be readily tuned by changing the temperature, electron density, scattering rate, and sample thickness. The edge frequency exhibited a surprising increase with decreasing temperature below 15 K, which we explain as a result of a weak-to-strong transition in the scattering rate, going from ωτ  ≫ 1 to ωτ ∼ 1. These results indicate that doped narrow-gap semiconductors provide a versatile platform for manipulating THz waves in a controllable manner, especially as a high-pass filter with an unprecedented on/off ratio.
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http://dx.doi.org/10.1364/OE.418624DOI Listing
March 2021

High-Pressure O Annealing Enhances the Crystallinity of Ultrawide-Band-Gap Sesquioxides Combined with Graphene for Vacuum-Ultraviolet Photovoltaic Detection.

ACS Appl Mater Interfaces 2021 Apr 31;13(14):16660-16668. Epub 2021 Mar 31.

School of Materials, Sun Yat-sen University, Guangzhou, Guangdong 510275, China.

(AlGa)O is emerging as a promising wide-band-gap sesquioxide for vacuum-ultraviolet (VUV, 10-200 nm) photodetectors and high-power field-effect transistors. However, how the key parameters such as the band gap and crystalline phase of the (AlGa)O-based device vary with stoichiometry has not been explicitly defined, which is due to the unclear underlying mechanism of the Al local coordination environment. In this work, a high-pressure O (20 atm) annealing (HPOA) strategy that can significantly improve the crystallinity of β-(AlGa)O and achieve a tunable optical band gap was proposed, facilitating the revelation of the local structure of Al varying with Al content and the kinetic mechanism of Al diffusion. By combining the as-HPOA-treated single-crystalline β-(AlGa)O films with p-type graphene (p-Gr), which serves as a transparent conductor, a VUV photovoltaic detector is fabricated, showing an improved photovoltage (0.80 V) and fast temporal response (2.1 μs). All of these findings provide a rewarding and important strategy for enhancing the band-gap tunability of sesquioxides, as well as the flexibility of zero-power-consumption photodetectors.
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http://dx.doi.org/10.1021/acsami.1c00429DOI Listing
April 2021

Total flavonoids of rhizoma drynariae ameliorates bone formation and mineralization in BMP-Smad signaling pathway induced large tibial defect rats.

Biomed Pharmacother 2021 Jun 26;138:111480. Epub 2021 Mar 26.

Department of Orthopaedics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China. Electronic address:

Osteogenesis and angiogenesis acts as an essential role in repairing large tibial defects (LTDs). Total flavonoids of rhizoma drynariae (TFRD), a traditional Chinese medicinal herb, is reported to show anabolic effects on fracture healing. However, whether TFRD could improve the bone formation and angiogenesis in LTDs remains unknown. The purpose of this study was to evaluate the effect of TFRD on bone formation and angiogenesis in LTDs in distraction osteogenesis (DO). Using a previously established fracture model, LTD rats was established with circular external fixator (CEF). All rats then randomly divided into TFRD low dosage group (with DO), TFRD medium dosage group (with DO), TFRD high dosage group (with DO), model group (with DO) and blank group (without DO). Twelve weeks after treatment, according to X-ray and Micro-CT, TFRD groups (especially in medium dosage group) can significantly promote the formation of a large number of epiphyses and improve new bone mineralization compared with model group, and the results of HE and Masson staining and in vitro ALP level of BMSC also demonstrated the formation of bone matrix and mineralization in the TFRD groups. Also, angiographic imaging suggested that total flavonoids of TFRD was able to promote angiogenesis in the defect area. Consistently, TFRD significantly increased the levels of BMP-2, SMAD1, SMAD4, RUNX-2, OSX and VEGF in LTD rats based on ELISA and Real-Time PCR. In addition, we found that ALP activity of TFRD medium dosage group reached a peak after 10 days of induction through BMSC cell culture in vitro experiment. TFRD promoted bone formation in LTD through activation of BMP-Smad signaling pathway, which provides a promising new strategy for repairing bone defects in DO surgeries.
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http://dx.doi.org/10.1016/j.biopha.2021.111480DOI Listing
June 2021

Characteristics Associated with the Occurrence and Development of Acute Anterior Uveitis, Inflammatory Bowel Disease, and Psoriasis in Patients with Ankylosing Spondylitis: Data from the Chinese Ankylosing Spondylitis Prospective Imaging Cohort.

Rheumatol Ther 2021 Mar 11;8(1):555-571. Epub 2021 Mar 11.

Department of Rheumatology, Chinese PLA General Hospital, Beijing, 100853, China.

Introduction: This study aimed to determine the association between extra-articular manifestations (EAMs) and baseline characteristics of patients with ankylosing spondylitis (AS) and identify their potential risk factors in an observational cohort.

Methods: We analyzed the data of consecutive patients with AS obtained between April 2016 and May 2019 from the ongoing Chinese Ankylosing Spondylitis Prospective Imaging Cohort.

Results: Among the 1414 patients with AS, 23.1% had experienced EAMs at baseline. The prevalence rates of acute anterior uveitis (AAU), inflammatory bowel disease, and psoriasis among patients with AS were 16.7, 6.9, and 2.6%, respectively, and the prevalence of AAU increased significantly with the disease duration. Patients with comorbidity of AAU and psoriasis had Ankylosing Spondylitis Disease Activity Score (ASDAS) than patients without EAMs (2.16 ± 0.984 vs. 1.99 ± 0.956 [p = 0.025] and 2.36 ± 1.01 vs. 1.99 ± 0.96 [p = 0.025]). Among the 1087 patients with AS without EAMs at baseline, 98 developed EAMs during follow-up. Long disease duration (> 10 years) and high disease activity at baseline (ASDAS > 2.1) were associated with the risk of new-onset EAMs (hazard ratio [HR] [95% confidence interval, CI], 2.150 [1.229-3.762] and 2.896 [1.509-5.561], respectively) and new-onset AAU (HR [95% CI], 2.197 [1.325-3.642] and 3.717 [1.611-8.574], respectively).

Conclusions: In Chinese patients with AS, patients with comorbidity of AAU and psoriasis had higher disease activity scores than patients without EAMs. Furthermore, the risk of AAU or combined EAMs increases with the duration of AS and appears to be associated with higher cumulative exposure to inflammation.
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http://dx.doi.org/10.1007/s40744-021-00293-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991047PMC
March 2021

Lymph node metastasis-derived gastric cancer cells educate bone marrow-derived mesenchymal stem cells via YAP signaling activation by exosomal Wnt5a.

Oncogene 2021 Mar 2;40(12):2296-2308. Epub 2021 Mar 2.

Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, China.

Lymph node metastasis (LNM), a common metastatic gastric-cancer (GC) route, is closely related to poor prognosis in GC patients. Bone marrow-derived mesenchymal stem cells (BM-MSCs) preferentially engraft at metastatic lesions. Whether BM-MSCs are specifically reprogrammed by LNM-derived GC cells (LNM-GCs) and incorporated into metastatic LN microenvironment to prompt GC malignant progression remains unknown. Herein, we found that LNM-GCs specifically educated BM-MSCs via secretory exosomes. Exosomal Wnt5a was identified as key protein mediating LNM-GCs education of BM-MSCs, which was verified by analysis of serum exosomes collected from GC patients with LNM. Wnt5a-enriched exosomes induced YAP dephosphorylation in BM-MSCs, whereas Wnt5a-deficient exosomes exerted the opposite effect. Inhibition of YAP signaling by verteporfin blocked LNM-GC exosome- and serum exosome-mediated reprogramming in BM-MSCs. Analysis of MSC-like cells obtained from metastatic LN tissues of GC patients (GLN-MSCs) confirmed that BM-MSCs incorporated into metastatic LN microenvironment, and that YAP activation participated in maintaining their tumor-promoting phenotype and function. Collectively, our results show that LNM-GCs specifically educated BM-MSCs via exosomal Wnt5a-elicited activation of YAP signaling. This study provides new insights into the mechanisms of LNM in GC and BM-MSC reprogramming, and will provide potential therapeutic targets and detection indicators for GC patients with LNM.
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http://dx.doi.org/10.1038/s41388-021-01722-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994201PMC
March 2021

Exosomal proteins: Key players mediating pre‑metastatic niche formation and clinical implications (Review).

Int J Oncol 2021 04 2;58(4). Epub 2021 Mar 2.

Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.

Tumor metastasis is a destructive characteristic of malignant tumors and the fundamental reason why malignant tumors are difficult to cure. The concept of a pre‑metastatic niche (PMN) provides a novel way to elucidate the molecular mechanism of tumor metastasis. At present, the PMN has been considered as a critical determinant priming distal sites for metastasis. Accumulating evidence has suggested that exosomes are cellular communicators serving a pivotal role in mediating tumor cell metastasis by establishing the PMN. Among exosomal cargos, non‑coding RNAs and proteins are two commonly studied components; however, the latter has received less attention. The present review aimed to summarize the findings regarding cargo proteins selectively loaded in malignant tumor‑derived exosomes. Metastasis‑associated proteins have been demonstrated to be selectively enriched in malignant tumor‑derived exosomes. Exosomal proteins promote PMN formation to mediate the site‑specific metastasis of tumor cells by inducing lymphangiogenesis, angiogenesis and permeability, educating stromal cells, remodeling the extracellular matrix, and suppressing the antitumor immune response. These exosomal proteins have great potential in predicting organ‑directed metastasis and prognosis, as well as in cancer therapy.
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http://dx.doi.org/10.3892/ijo.2021.5184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895540PMC
April 2021

Clinical manifestations and outcome of anti-aminoacyl-transfer RNA synthetase antibody and anti-melanoma differentiation-associated gene 5 antibody positive patients with interstitial lung disease.

Clin Exp Rheumatol 2021 Feb 15. Epub 2021 Feb 15.

Department of Rheumatology and Immunology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Objectives: To analyse the clinical features and risk factors of acute/subacute interstitial pneumonia (A/SIP) and death in patients with positive anti-aminoacyl-transfer RNA synthetase antibody (anti-ARS Ab) and positive anti-melanoma differentiation-associated gene 5 antibodies (anti-MDA5 Ab).

Methods: Interstitial lung disease (ILD) patients with anti-ARS+ or anti-MDA5+ were recruited. Their demographics, clinical manifestations, laboratory data were collected and they were followed up for 1 year. Risk factors of A/SIP and mortality were analysed.

Results: 71 patients with anti-ARS+ ILD and 31 patients with anti-MDA5+ ILD were included. Incidence of ulcerative rash, Gottron's sign, pulmonary infection and A/SIP in the anti-MDA5+ group were significantly higher than those in the anti-ARS+ group, Creatine kinase (CK), leukocyte count, and lymphocyte count were lower, the value of serum ferritin (SF) was higher, and 12-month cumulative survival rate was lower. Advanced age, anti-MDA5+ and low immunoglobulin G (IgG) level were independent predictors of A/SIP. The decreased PaO2 and elevated SF were independent predictors for poor prognosis in A/SIP patients.

Conclusions: Compared to anti-ARS+ group, the anti-MDA5+ group was more prone to ulcerative rash, Gottron's sign and pulmonary infection. Patients with anti-MDA5+, advanced age and decreased values of IgG were more likely to have A/SIP, while patients with A/SIP had lower incidence of myositis and arthritis. Mortality of A/SIP patients increased with higher serum ferritin level.
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February 2021

Fecal microbiota transplantation for rheumatoid arthritis: A case report.

Clin Case Rep 2021 Feb 23;9(2):906-909. Epub 2020 Dec 23.

Department of Gastroenterology and Hepatology The First Medical Center Chinese PLA General Hospital Beijing China.

No previous case of using fecal microbiota transplantation (FMT) to treat rheumatoid arthritis (RA) has been reported. We report a case of a patient with refractory RA successfully treated with FMT indicating that FMT may have a good therapeutic effect on RA.
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http://dx.doi.org/10.1002/ccr3.3677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869316PMC
February 2021

A guaranteed convergence analysis for the projected fast iterative soft-thresholding algorithm in parallel MRI.

Med Image Anal 2021 04 1;69:101987. Epub 2021 Feb 1.

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, School of Electronic Science and Engineering, National Model Microelectronics College, Xiamen University, Xiamen 361005, China. Electronic address:

Sparse sampling and parallel imaging techniques are two effective approaches to alleviate the lengthy magnetic resonance imaging (MRI) data acquisition problem. Promising data recoveries can be obtained from a few MRI samples with the help of sparse reconstruction models. To solve the optimization models, proper algorithms are indispensable. The pFISTA, a simple and efficient algorithm, has been successfully extended to parallel imaging. However, its convergence criterion is still an open question. Besides, the existing convergence criterion of single-coil pFISTA cannot be applied to the parallel imaging pFISTA, which, therefore, imposes confusions and difficulties on users about determining the only parameter - step size. In this work, we provide the guaranteed convergence analysis of the parallel imaging version pFISTA to solve the two well-known parallel imaging reconstruction models, SENSE and SPIRiT. Along with the convergence analysis, we provide recommended step size values for SENSE and SPIRiT reconstructions to obtain fast and promising reconstructions. Experiments on in vivo brain images demonstrate the validity of the convergence criterion.
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http://dx.doi.org/10.1016/j.media.2021.101987DOI Listing
April 2021

Central moments multiple relaxation time LBM for hemodynamic simulations in intracranial aneurysms: An in-vitro validation study using PIV and PC-MRI.

Comput Biol Med 2021 Apr 3;131:104251. Epub 2021 Feb 3.

Laboratory of Fluid Dynamics and Technical Flows, University of Magdeburg "Otto von Guericke", D-39106, Magdeburg, Germany.

The lattice Boltzmann method (LBM) has recently emerged as an efficient alternative to classical Navier-Stokes solvers. This is particularly true for hemodynamics in complex geometries. However, in its most basic formulation, i.e. with the so-called single relaxation time (SRT) collision operator, it has been observed to have a limited stability domain in the Courant/Fourier space, strongly constraining the minimum time-step and grid size. The development of improved collision models such as the multiple relaxation time (MRT) operator in central moments space has tremendously widened the stability domain, while allowing to overcome a number of other well-documented artifacts, therefore opening the door for simulations over a wider range of grid and time-step sizes. The present work focuses on implementing and validating a specific collision operator, the central Hermite moments multiple relaxation time model with the full expansion of the equilibrium distribution function, to simulate blood flows in intracranial aneurysms. The study further proceeds with a validation of the numerical model through different test-cases and against experimental measurements obtained via stereoscopic particle image velocimetry (PIV) and phase-contrast magnetic resonance imaging (PC-MRI). For a patient-specific aneurysm both PIV and PC-MRI agree fairly well with the simulation. Finally, low-resolution simulations were shown to be able to capture blood flow information with sufficient accuracy, as demonstrated through both qualitative and quantitative analysis of the flow field while leading to strongly reduced computation times. For instance in the case of the patient-specific configuration, increasing the grid-size by a factor of two led to a reduction of computation time by a factor of 14 with very good similarity indices still ranging from 0.83 to 0.88.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104251DOI Listing
April 2021

Continuous production of fructooligosaccharides by recycling of the thermal-stable β-fructofuranosidase produced by Aspergillus niger.

Biotechnol Lett 2021 Jun 11;43(6):1175-1182. Epub 2021 Feb 11.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, 266237, People's Republic of China.

Objective: To achieve continuous production of fructooligosaccharides (FOS) by recycling of the mycelial cells containing the thermal-stable β-fructofuranosidase in Aspergillus niger without immobilization.

Results: The thermal-stable β-fructofuranosidase FopA-V1 was successfully expressed in A. niger ATCC 20611 under the control of the constitutive promoter PgpdA. The engineered A. niger strain FV1-11 produced the β-fructofuranosidase with improved thermostability, which remained 91.2% of initial activity at 50 °C for 30 h. Then its mycelial β-fructofuranosidase was recycled for the synthesis of FOS. It was found that the enzyme still had 79.3% of initial activity after being reused for six consecutive cycles, whereas only 62.3% β-fructofuranosidase activity was detected in the parental strain ATCC 20611. Meanwhile, the FOS yield of FV1-11 after six consecutive cycles reached 57.1% (w/w), but only 51.0% FOS yield was detected in ATCC 20611.

Conclusions: The thermal-stable β-fructofuranosidase produced by A. niger can be recycled to achieve continuous synthesis of FOS with high efficiency, providing a powerful and economical strategy for the industrial production of FOS.
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http://dx.doi.org/10.1007/s10529-021-03099-wDOI Listing
June 2021

A Rapid and Robust Light-and-Solution-Triggered In Situ Crafting of Organic Passivating Membrane over Metal Halide Perovskites for Markedly Improved Stability and Photocatalysis.

Nano Lett 2021 Feb 11;21(4):1643-1650. Epub 2021 Feb 11.

State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials, Sun Yat-Sen University, Guangzhou 510275, China.

Despite intriguing optoelectronic attributes in solar cells, light-emitting diodes, and photocatalysis, the instability of organic-inorganic perovskites poises a grand challenge for long-term applications. Herein, we report a simple yet robust strategy via light-and-solution treatment to create an organic membrane that effectively passivates CHNHPbI (MAPbI). Specifically, the restructuring of MA is observed on MAPbI in aqueous hydrogen iodide. HIO molecules are generated via the reaction between water and I induced by photocatalysis when MAPbI is illuminated. The hydrogen bonding between HIO molecules at different perovskite particles not only directs the creeplike growth of perovskite particles but also in situ forms a passivating layer firmly anchoring on the perovskite surface with hydrophilic -NH groups tethering to perovskites and hydrophobic -CH moieties exposed to air. Intriguingly, such MA film greatly improves the stability of perovskites against moisture as well as their crystal quality, considerably enhancing the photocatalytic hydrogen evolution rate.
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http://dx.doi.org/10.1021/acs.nanolett.0c04299DOI Listing
February 2021

Safety and Efficacy of Prefilled Liquid Etanercept-Biosimilar Yisaipu for Active Ankylosing Spondylitis: A Multi-Center Phase III Trial.

Rheumatol Ther 2021 Mar 9;8(1):361-374. Epub 2021 Feb 9.

Department of Rheumatology and Immunology, Chinese PLA General Hospital, Beijing, China.

Introduction: The aim of this work is to examine the efficacy and safety of prefilled liquid etanercept-biosimilar Yisaipu versus lyophilized Yisaipu in active ankylosing spondylitis (AS) patients.

Methods: This double-blind, phase III trial with non-inferiority design randomized adult patients with active AS in a 3:1:1 ratio to receive twice-weekly 25-mg prefilled liquid Yisaipu for a total of 48 injections (group I, n = 330), once-weekly 50-mg prefilled liquid Yisaipu for 24 injections (group II, n = 110), or twice-weekly 25-mg lyophilized Yisaipu for 48 injections (group III, n = 110). Both physicians and patients who received 25-mg twice-weekly lyophilized or liquid Yisaipu were blinded to treatment assignment while patients who received 50-mg once-weekly liquid Yisaipu received treatment in an open-label design. In addition, 90 patients in the PK/PD study were randomized in a 1:1:1 ratio to each group. The primary outcome was the proportion of patients who achieved ASAS20 at week 24.

Results: A total of 640 subjects were enrolled. The proportion of patients who attained ASAS20 at week 24 was 85.56% in group I, 85.71% in group II, and 83.45% in group III (group I vs. III, P = 0.545; group II vs. III, P = 0.605). The difference between group I and III was 2.10% (95% CI - 5.06%, 9.27%) and 2.26% (95% CI - 6.21%, 10.73%) between group II and III, meeting the non-inferiority threshold (Δ = - 15%) (P < 0.001). Except for a statistical difference between group I (75.83%) and group III at week 8 (64.75%, P = 0.011), there was no statistical difference in the ASAS20 attainment rate among the three groups at other time points. The incidence of serious adverse events was comparable among the three groups (group I, 2.50%, II, 2.86% and III, 1.43%; P > 0.05). No deaths were reported.

Conclusions: Once-weekly 50-mg or twice-weekly 25-mg prefilled liquid Yisaipu is safe and non-inferior to twice-weekly 25-mg lyophilized Yisaipu.

Trial Registration: CTR20130124 and NCT04345458.
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http://dx.doi.org/10.1007/s40744-021-00276-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991065PMC
March 2021

γ-Synuclein is Closely Involved in Autophagy that Protects Colon Cancer Cell from Endoplasmic Reticulum Stress.

Anticancer Agents Med Chem 2021 Jan 18. Epub 2021 Jan 18.

Department of Gastrointestinal Tumor Surgery, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital; Fujian Province Key Laboratory of Tumor Biotherapy, Fuzhou, Fujian Province, 350014. China.

Background: In previous studies, we provided evidence suggesting the involvement of γ-synuclein in growth, invasion, and metastasis of colon cancer cells in vitro and in vivo. Among γ-synuclein downstream genes, the microtubule-associated protein 1 light chain 3 (LC3), an autophagy gene, was screened by gene expression profile chip analysis.

Objective: We planned to investigate the functional effects of γ-synuclein on autophagy induced by ER stress in colon cancer cells.

Methods: We investigated the functional effects of γ-synuclein on autophagy and apoptosis induced by Thapsigargin (TG), ER stressinducing agent, in colon cancer cell lines using immunofluorescence staining, RT-PCR, western blot, CCK8 test, flow cytometry analysis, and transmission electron microscopy. To further determine how γ-synuclein regulated autophagy and apoptosis, PD98059 (ERK inhibitor), SP600125 (ERK inhibitor), anisomycin (JNK activator), and c-Jun siRNA were used respectively in γ-synuclein siRNA transfected HCT116 cells. Then, autophagy proteins, apoptosis proteins, and pathway proteins were detected by western blot analysis. The expression of autophagy genes was assessed by RT-PCR.

Results: Our data showed that ER stress-induced colon cancer cells autophagy mainly in the early stage (0-24h) and apoptosis mainly in the late stage (24-48h). ER stress up-regulated γ-synuclein gene and protein expression in colon cancer cells, accompanied by autophagy. γ-synuclein protected HCT116 cells by enhancing autophagy in the early stage (0-24h) through activation of ERK and JNK pathway and inhibiting apoptosis in the late stage (24-48h) through inhibition of the JNK pathway. γ-synuclein could promote autophagy via the JNK pathway activation of ATG genes, LC3, Beclin 1, and ATG7. γ-synuclein may play a role in the transition between autophagy and apoptosis in our model.

Conclusion: Overall, we provided the first experimental evidence to show that γ-synuclein may play an important role in autophagy that protects colon cancer cells from ER stress. Therefore, our data suggest a new molecular mechanism for γ-synuclein-mediated CRC progression.
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http://dx.doi.org/10.2174/1871520621666210119093327DOI Listing
January 2021

Induced regulatory T cells suppress Tc1 cells through TGF-β signaling to ameliorate STZ-induced type 1 diabetes mellitus.

Cell Mol Immunol 2021 Mar 14;18(3):698-710. Epub 2021 Jan 14.

Department of Endocrinology and Metabolism, Guangdong Provincial Key Laboratory of Diabetology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, Guangdong, China.

Type 1 diabetes mellitus (T1D) is a chronic autoimmune condition in which the immune system destroys insulin-producing pancreatic β cells. In addition to well-established pathogenic effector T cells, regulatory T cells (Tregs) have also been shown to be defective in T1D. Thus, an increasing number of therapeutic approaches are being developed to target Tregs. However, the role and mechanisms of TGF-β-induced Tregs (iTregs) in T1D remain poorly understood. Here, using a streptozotocin (STZ)-induced preclinical T1D mouse model, we found that iTregs could ameliorate the development of T1D and preserve β cell function. The preventive effect was associated with the inhibition of type 1 cytotoxic T (Tc1) cell function and rebalancing the Treg/Tc1 cell ratio in recipients. Furthermore, we showed that the underlying mechanisms were due to the TGF-β-mediated combinatorial actions of mTOR and TCF1. In addition to the preventive role, the therapeutic effects of iTregs on the established STZ-T1D and nonobese diabetic (NOD) mouse models were tested, which revealed improved β cell function. Our findings therefore provide key new insights into the basic mechanisms involved in the therapeutic role of iTregs in T1D.
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http://dx.doi.org/10.1038/s41423-020-00623-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027661PMC
March 2021

Delayed diagnosis of prosopagnosia following a hemorrhagic stroke in an elderly man: A case report.

World J Clin Cases 2020 Dec;8(24):6487-6498

Department of Geriatric Medicine, Fujian Provincial Hospital, Fuzhou 350001, Fujian Province, China.

Background: Acquired prosopagnosia is a rare condition characterized by the loss of familiarity with previously known faces and the inability to recognize new ones. It usually occurs after the onset of brain lesions such as in a stroke. The initial identification of prosopagnosia generally relies on a patient's self-report, which can be challenging if it lacks an associated chief complaint. There were few cases of prosopagnosia presenting purely as eye symptoms in the previous literature confirmed by functional magnetic resonance imaging (MRI).

Case Summary: We present a case of delayed diagnosis of prosopagnosia after a right hemisphere stroke in an elderly man whose chief complaint was persistent and progressive "blurred vision" without facial recognition impairment. Ophthalmic tests revealed a homonymous left upper quadrantanopia, with normal visual acuity. He was found by accident to barely recognize familiar faces. The patient showed severe deficit in face recognition and perception tests, and mild memory loss in neuropsychological assessments. Further functional MRI revealed the visual recognition deficits were face-specific. After behavioral intervention, the patient started to rely on other cues to compensate for poor facial recognition. His prosopagnosia showed no obvious improvement eight months after the stroke, which had negative impact on his social network.

Conclusion: Our case demonstrates that the presentation of prosopagnosia can be atypical, and visual difficulties might be a clinical manifestation solely of prosopagnosia, which emphasizes the importance of routinely considering face recognition impairment among elderly patients with brain lesions.
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http://dx.doi.org/10.12998/wjcc.v8.i24.6487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760442PMC
December 2020