Publications by authors named "Feng He"

1,076 Publications

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Cross-sectional comparisons of sodium content in processed meat and fish products among five countries: potential for feasible targets and reformulation.

BMJ Open 2021 Oct 14;11(10):e046412. Epub 2021 Oct 14.

Department of nutrition and food hygiene, Hebei Medical University, Shijiazhuang, China

Introduction: Reducing sodium intake has been identified as a highly cost-effective strategy to prevent and control high blood pressure and reduce cardiovascular mortality. This study aims to compare the sodium content in processed meat and fish products among five countries, which will contribute to the evidence-base for feasible strategies of sodium reduction in such products.

Methods: Sodium content on product labels of 26 500 prepackaged products, 19 601 meat and 6899 fish, was collected in supermarkets from five countries using the FoodSwitch mobile application from 2012 to 2018. To be specific, it was 1898 products in China, 885 in the UK, 5673 in Australia, 946 in South Africa and 17 098 in the USA. Cross-sectional comparisons of sodium levels and proportions meeting 2017 UK sodium reduction targets were conducted using Kruskal-Wallis H and the χ test, respectively across the five countries.

Results: The results showed that processed meat and fish products combined in China had the highest sodium level (median 1050 mg/100 g, IQR: 774-1473), followed by the USA, South Africa, Australia, with the lowest levels found in UK (432 mg/100 g, IQR: 236-786) (p<0.001). Similar variations, that is, a twofold to threefold difference of sodium content between the highest and the lowest countries were found among processed meat and fish products separately. Large sodium content variations were also found in certain specific food subcategories across the five countries, as well as across different food subcategories within each country.

Conclusion: Processed meat and fish products differ greatly in sodium content across different countries and across different food subcategories. This indicates great potential for food producers to reformulate the products in sodium content, as well as for consumers to select less salted food.
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http://dx.doi.org/10.1136/bmjopen-2020-046412DOI Listing
October 2021

Over 21% Efficiency Stable Two-dimensional Perovskite Solar Cells.

Adv Mater 2021 Oct 14:e2107211. Epub 2021 Oct 14.

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China.

Owing to the insufficient light absorption and charge transport, two-dimensional(2D) Ruddlesden-Popper (RP) perovskites show the relatively low efficiency. Here, we fabricated the methylammonium(MA), formamidinum(FA) and FA/MA mixed 2D PSCs. Incorporating FA cations extends the absorption range and enhance the light absorption. Optical spectroscopy showed that FA cations substantially increased the portion of 3D-like phase to 2D phases, and XRD studies revealed that FA based 2D perovskite possessed an oblique crystal orientation. Nevertheless, the ultra-fast interphase charge transfer result in an extremely long carrier diffusion length (∼1.98 μm). Also, chloride additives effectively suppress the yellow δ-phase formation of pure FA based 2D PSCs. As a result, both FA/MA mixed and pure FA based 2D PSCs exhibit a greatly enhanced PCE over 20%. Specifically, the pure FA based 2D PSCs achieved a record PCE of 21.07% (certified at 20%), which is the highest efficiency for low-dimensional PSCs (n≤10) reported to date. Importantly, the FA based 2D PSCs retain 97% of its initial efficiency at 85°C persistent heating after 1500 hrs. Our results unambiguously demonstrate that pure FA based 2D PSCs are promising to achieve comparable efficiency to the 3D perovskite, along with a better device stability. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202107211DOI Listing
October 2021

Discovery of 2-(Ortho-Substituted Benzyl)-Indole Derivatives as Potent and Orally Bioavailable RORγ Agonists with Antitumor Activity.

J Med Chem 2021 Oct 13. Epub 2021 Oct 13.

Eternity Bioscience Inc., 6 Cedarbrook Drive, Cranbury, New Jersey 08512, United States.

RORγ is a dual-functional drug target, which involves not only induction of inflammation but also promotion of cancer immunity. The development of agonists of RORγ promoting Th17 cell differentiation could provide a novel mechanism of action (MOA) as an immune-activating anticancer agent. Herein, we describe new 2-(ortho-substituted benzyl)-indole derivatives as RORγ agonists by scaffold hopping based on clinical RORγ antagonist VTP-43742. Interestingly, subtle structural differences of the compounds led to the opposite biological MOA. After rational optimization for structure-activity relationship and pharmacokinetic profile, we identified a potent RORγ agonist compound that was able to induce the production of IL-17 and IFNγ in tumor tissues and elicit antitumor efficacy in MC38 syngeneic mouse colorectal tumor model. This is the first comprehensive work to demonstrate the antitumor efficacy of an RORγ agonist.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00828DOI Listing
October 2021

Preparation and Antibacterial Activity of Thermo-Responsive Nanohydrogels from Qiai Essential Oil and Pluronic F108.

Molecules 2021 Sep 23;26(19). Epub 2021 Sep 23.

Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, China.

Essential oils (EOs) have been used in cosmetics and food due to their antimicrobial and antiviral effects. However, the applications of EOs are compromised because of their poor aqueous solubility and high volatility. Qiai ( Levl. et Van. var. cv. Qiai) is a traditional Chinese herb and possesses strong antibacterial activity. Herein, we report an innovative formulation of EO as nanohydrogels, which were prepared through co-assembly of Qiai EO (QEO) and Pluronic F108 (PEG--PPG--PEG, or PF108) in aqueous solution. QEO was efficiently loaded in the PF108 micelles and formed nanohydrogels by heating the QEO/PF108 mixture solution to 37 °C, by the innate thermo-responsive property of PF108. The encapsulation efficiency and loading capacity of QEO reached 80.2% and 6.8%, respectively. QEO nanohydrogels were more stable than the free QEO with respect to volatilization. Sustained QEO release was achieved at body temperature using the QEO nanohydrogels, with the cumulative release rate reaching 95% in 35 h. In vitro antibacterial test indicated that the QEO nanohydrogels showed stronger antimicrobial activity against and than the free QEO due to the enhanced stability and sustained-release characteristics. It has been attested that thermo-responsive QEO nanohydrogels have good potential as antibacterial cosmetics.
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http://dx.doi.org/10.3390/molecules26195771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510472PMC
September 2021

Improve P300-Speller performance by online adaptive tuning Stimulus Onset Asynchrony (SOA).

J Neural Eng 2021 Oct 12. Epub 2021 Oct 12.

Tianjin University School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjing, 300072, CHINA.

The P300-Speller is a classic brain-computer interface (BCI) paradigm that has been subjected to numerous clinical trials. Some studies have reported that the performance of the P300-Speller is closely related to Stimulus Onset Asynchrony (SOA), but very few studies have attempted to improve the performance of the P300-Speller by optimizing SOA. In this paper, we designed a P300-Speller system based on a dynamic SOA and dynamic stop strategy (DSS), which can automatically adjust SOA according to real-time operational performance. The online experiment results of 18 subjects showed that the event-related potential (ERP) classifier and the dynamic stop algorithm established at 200ms SOA can maintain the performance at a certain level among 50ms-300ms SOA. The system can then reduce the SOA from an initial 200 ms to an average of about 98.5 ms while maintaining letter output accuracy. The average theoretical information transfer rate (ITR) was significantly improved from 42.4 bit/min to 85 bit/min (the maximum was 232 bit/min). These results demonstrate that the system established in this paper can automatically optimize the SOA settings, and this personalized SOA adjustment can effectively improve the performance of the P300-Speller.
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http://dx.doi.org/10.1088/1741-2552/ac2f04DOI Listing
October 2021

Characterization of the early gene expression profile in Populus ussuriensis under cold stress using PacBio SMRT sequencing integrated with RNA-seq reads.

Tree Physiol 2021 Oct 9. Epub 2021 Oct 9.

State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.

Populus ussuriensis is an important and fast-growing afforestation plant species in north-eastern China. The whole-genome sequencing of P. ussuriensis has not been completed. Also, the transcriptional network of P. ussuriensis response to cold stress remains unknown. To unravel the early response of P. ussuriensis to chilling (3°C) stress and freezing (-3°C) stresses at the transcriptional level, we performed Single-Molecule Real-Time (SMRT) and Illumina RNA sequencing for P. ussuriensis. SMRT long-read isoform sequencing led to the identification of 29,243,277 subreads, and 575,481 circular consensus sequencing (CCS) reads. Approximately 50,910 high-quality (HQ) isoforms were generated, and 2,272 simple sequence repeats (SSRs), 8,086 long non-coding RNAs (lncRNAs) were identified. The Ca2+ content and abscisic acid (ABA) content in P. ussuriensis were significantly increased under cold stresses, while the value in freezing stress treatment group was significantly higher than chilling stress treatment group. A total of 49 genes that are involved in the signal transduction pathways related to perception and transmission of cold stress signals, such as Ca2+ signaling pathway, ABA signaling pathway, and MAPK signaling cascade, were found to be differentially expressed. In addition, 158 transcription factors (TFs) from 21 different families, such as MYB, WRKY, and AP2/ERF, were differentially expressed during chilling and freezing treatments. Moreover, the measurement of physiological indicators and bioinformatics observations demonstrated the altered expression pattern of genes involved in reactive oxygen species (ROS) balance, and sugar metabolism pathway during chilling and freezing stresses. This is the first time to report the early responses of P. ussuriensis to cold stress, which lays the foundation for future studies on the regulatory mechanisms in cold stress response. Besides, the full-length reference transcriptome of P. ussuriensis deciphered could be used in future studies on P. ussuriensis.
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http://dx.doi.org/10.1093/treephys/tpab130DOI Listing
October 2021

Effect of Stimulus Frequency on Air-Conducted Vestibular Evoked Myogenic Potentials.

J Int Adv Otol 2021 Sep;17(5):422-425

Department of Geriatrics, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China.

Objective: The aim of this study is to explore the effect of stimulus frequency on air-conducted cervical and ocular vestibular evoked myogenic potential (cVEMP and oVEMP) in healthy subjects.

Methods: The study included 45 healthy subjects who underwent the VEMP tests. Different stimulus frequencies (250-1500 Hz) were used for air-conducted cVEMP and oVEMP.

Results: In cVEMP, P1 and N1 latencies were significantly affected by different frequencies (P < .01). The amplitude at 500 Hz was significantly larger than those at other frequencies (P < .01).There was no significant main effect of frequency on asymmetry ratio (AR) (P > .05). In oVEMP, there was a tendency for the N1 and P1 latencies to decrease from 250 Hz to 1500 Hz (P < .01). The amplitudes at 500 Hz and 1000 Hz were significantly larger than the amplitudes at 250 Hz and 1500 Hz (P < .01).There was no significant main effect of frequency on AR (P > .05).

Conclusion: The optimal stimulus frequency of the cVEMP is 500 Hz and for the oVEMP is 500Hz or 1000Hz. Due to the absence of impact of stimulus frequency, AR is the best parameter of VEMP for clinical use.
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http://dx.doi.org/10.5152/iao.2021.8836DOI Listing
September 2021

Impact of color-coded and warning nutrition labelling schemes: A systematic review and network meta-analysis.

PLoS Med 2021 Oct 5;18(10):e1003765. Epub 2021 Oct 5.

Wolfson Institute of Population Health, Barts and The London School of Medicine & Dentistry, Queen Mary University of London, London, United Kingdom.

Background: Suboptimal diets are a leading risk factor for death and disability. Nutrition labelling is a potential method to encourage consumers to improve dietary behaviour. This systematic review and network meta-analysis (NMA) summarises evidence on the impact of colour-coded interpretive labels and warning labels on changing consumers' purchasing behaviour.

Methods And Findings: We conducted a literature review of peer-reviewed articles published between 1 January 1990 and 24 May 2021 in PubMed, Embase via Ovid, Cochrane Central Register of Controlled Trials, and SCOPUS. Randomised controlled trials (RCTs) and quasi-experimental studies were included for the primary outcomes (measures of changes in consumers' purchasing and consuming behaviour). A frequentist NMA method was applied to pool the results. A total of 156 studies (including 101 RCTs and 55 non-RCTs) nested in 138 articles were incorporated into the systematic review, of which 134 studies in 120 articles were eligible for meta-analysis. We found that the traffic light labelling system (TLS), nutrient warning (NW), and health warning (HW) were associated with an increased probability of selecting more healthful products (odds ratios [ORs] and 95% confidence intervals [CIs]: TLS, 1.5 [1.2, 1.87]; NW, 3.61 [2.82, 4.63]; HW, 1.65 [1.32, 2.06]). Nutri-Score (NS) and warning labels appeared effective in reducing consumers' probability of selecting less healthful products (NS, 0.66 [0.53, 0.82]; NW,0.65 [0.54, 0.77]; HW,0.64 [0.53, 0.76]). NS and NW were associated with an increased overall healthfulness (healthfulness ratings of products purchased using models such as FSAm-NPS/HCSP) by 7.9% and 26%, respectively. TLS, NS, and NW were associated with a reduced energy (total energy: TLS, -6.5%; NS, -6%; NW, -12.9%; energy per 100 g/ml: TLS, -3%; NS, -3.5%; NW, -3.8%), sodium (total sodium/salt: TLS, -6.4%; sodium/salt per 100 g/ml: NS: -7.8%), fat (total fat: NS, -15.7%; fat per 100 g/ml: TLS: -2.6%; NS: -3.2%), and total saturated fat (TLS, -12.9%; NS: -17.1%; NW: -16.3%) content of purchases. The impact of TLS, NS, and NW on purchasing behaviour could be explained by improved understanding of the nutrition information, which further elicits negative perception towards unhealthful products or positive attitudes towards healthful foods. Comparisons across label types suggested that colour-coded labels performed better in nudging consumers towards the purchase of more healthful products (NS versus NW: 1.51 [1.08, 2.11]), while warning labels have the advantage in discouraging unhealthful purchasing behaviour (NW versus TLS: 0.81 [0.67, 0.98]; HW versus TLS: 0.8 [0.63, 1]). Study limitations included high heterogeneity and inconsistency in the comparisons across different label types, limited number of real-world studies (95% were laboratory studies), and lack of long-term impact assessments.

Conclusions: Our systematic review provided comprehensive evidence for the impact of colour-coded labels and warnings in nudging consumers' purchasing behaviour towards more healthful products and the underlying psychological mechanism of behavioural change. Each type of label had different attributes, which should be taken into consideration when making front-of-package nutrition labelling (FOPL) policies according to local contexts. Our study supported mandatory front-of-pack labelling policies in directing consumers' choice and encouraging the food industry to reformulate their products.

Protocol Registry: PROSPERO (CRD42020161877).
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http://dx.doi.org/10.1371/journal.pmed.1003765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491916PMC
October 2021

Plastic response of L. root system traits and cold resistance to simulated rainfall events.

PeerJ 2021 9;9:e11962. Epub 2021 Sep 9.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Climate change (rainfall events and global warming) affects the survival of alfalfa ( L.) in winter. Appropriate water management can quickly reduce the mortality of alfalfa during winter. To determine how changes in water affect the cold resistance of alfalfa, we explored the root system traits under different rainfall events and the effects on cold resistance in three alfalfa cultivars. These were exposed to three simulated rainfall events (SRE) × two phases in a randomized complete block design with six replications. The three cultivars were WL168, WL353 and WL440, and the three SRE were irrigation once every second day (D), every four days (D) and every eight days (D). There were two phases: before cold acclimation and after cold acclimation. Our results demonstrated that a period of exposure to low temperature was required for alfalfa to achieve maximum cold resistance. The root system tended toward herringbone branching under D, compared with D and D, and demonstrated greater root biomass, crown diameter, root volume, average link length and topological index. Nevertheless, D had less lateral root length, root surface area, specific root length, root forks and fractal dimensions. Greater root biomass and topological index were beneficial to cold resistance in alfalfa, while more lateral roots and root forks inhibited its ability to survive winter. Alfalfa roots had higher proline, soluble sugar and starch content in D than in D and D. In contrast, there was lower malondialdehyde in D, indicating that alfalfa had better cold resistance following a longer irrigation interval before winter. After examining root biomass, root system traits and physiological indexes we concluded that WL168 exhibited stronger cold resistance. Our results contribute to greater understanding of root and cold stress, consequently providing references for selection of cultivars and field water management to improve cold resistance of alfalfa in the context of changes in rainfall patterns.
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http://dx.doi.org/10.7717/peerj.11962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435203PMC
September 2021

Ameliorating end-product inhibition to improve cadaverine production in engineered and its application in the synthesis of bio-based diisocyanates.

Synth Syst Biotechnol 2021 Dec 14;6(4):243-253. Epub 2021 Sep 14.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, Jiangsu, China Bbitechnology and Bioengineering.

Cadaverine is an important C5 platform chemical with a wide range of industrial applications. However, the cadaverine inhibition on the fermenting strain limited its industrial efficiency of the strain. In this study, we report an engineered strain with high cadaverine productivity that was generated by developing a robust host coupled with metabolic engineering to mitigate cadaverine inhibition. First, a lysine producing was treated with a combination of radiation (ultraviolet and visible spectrum) and ARTP (atmospheric and room temperature plasma) mutagenesis to obtain a robust host with high cadaverine tolerance. Three mutant targets including HokD, PhnI and PuuR are identified for improved cadaverine tolerance. Further transcriptome analysis suggested that cadaverine suppressed the synthesis of ATP and lysine precursor. Accordingly, the related genes involved in glycolysis and lysine precursor, as well as cadaverine exporter was engineered to release the cadaverine inhibition. The final engineered strain was fed-batch cultured and a titer of 58.7 g/L cadaverine was achieved with a yield of 0.396 g/g, both of which were the highest level reported to date in . The bio-based cadaverine was purified to >99.6% purity, and successfully used for the synthesis of polyurethane precursor 1,5-pentamethylene diisocyanate (PDI) through the approach of carbamate decomposition.
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http://dx.doi.org/10.1016/j.synbio.2021.09.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446744PMC
December 2021

Novel Serum and Urinary Metabolites Associated with Diabetic Retinopathy in Three Asian Cohorts.

Metabolites 2021 Sep 9;11(9). Epub 2021 Sep 9.

Singapore Eye Research Institute, Singapore National Eye Centre, Singapore 168751, Singapore.

Diabetic retinopathy (DR) is a microvascular complication of diabetes mellitus, a metabolic disorder, but understanding of its pathophysiology remains incomplete. Meta-analysis of three population-based cross-sectional studies (2004-11) representing three major Asian ethnic groups (aged 40-80 years: Chinese, 592; Malays, 1052; Indians, 1320) was performed. A panel of 228 serum/plasma metabolites and 54 urinary metabolites were quantified using nuclear magnetic resonance (NMR) spectroscopy. Main outcomes were defined as any DR, moderate/above DR, and vision-threatening DR assessed from retinal photographs. The relationship between metabolites and DR outcomes was assessed using multivariate logistic regression models, and metabolites significant after Bonferroni correction were meta-analyzed. Among serum/plasma metabolites, lower levels of tyrosine and cholesterol esters to total lipids ratio in IDL and higher levels of creatinine were positively associated with all three outcomes of DR (all < 0.005). Among urinary metabolites, lower levels of citrate, ethanolamine, formate, and hypoxanthine were positively associated with all three DR outcomes (all < 0.005). Higher levels of serum/plasma 3-hydroxybutyrate and lower levels of urinary 3-hydroxyisobutyrate were associated with VTDR. Comprehensive metabolic profiling in three large Asian cohorts with DR demonstrated alterations in serum/plasma and urinary metabolites mostly related to amino acids, lipoprotein subclasses, kidney function, and glycolysis.
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http://dx.doi.org/10.3390/metabo11090614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467425PMC
September 2021

Investigation of Diagnostic Biomarkers for Osteoporosis Based on Differentially Expressed Gene Profile with QCT and mDixon-Quant Techniques.

Orthop Surg 2021 Sep 13. Epub 2021 Sep 13.

Department of Biomedical Engineering, School of Precision Instrument and Optoelectronic Engineering, Tianjin University, Tianjin, China.

Objective: To develop a comprehensive differential expression profile for osteoporosis based on two independent data sources.

Methods: Using a hindlimb unloading (HLU) rat model to mimic osteoporosis syndrome in humans (animal experiments), the significant differentially expressed mRNAs in osteoporosis were analyzed using RNA-seq. The enriched GO terms as well as KEGG signaling pathways were also deeply investigated. Using clinical specimens to verify the functions of potential hub genes (biomarkers) for osteoporosis (clinical experiments), 128 suspected cases for osteoporosis from January 2019 to December 2020 were randomly selected and analyzed by quantitative computed tomography (QCT) as well as modified Dixon quantification (mDixon-Quant) techniques in the Tianjin hospital. Among these, 80 patients out of 128 suspected cases were finally diagnosed as the osteoporosis group. Meanwhile, 48 patients were selected for osteopenia group. There was no significant age and gender difference across participant subgroups. The protein levels of potential hub genes (FST, CCL3, and RAPGEF4) were determined by ELISA double antibody sandwich method for osteopenia and osteoporosis groups from peripheral blood.

Result: In the RNA-seq analysis, compared with control group, a total of 803 differentially expressed mRNAs were identified, including 288 up-regulated and 515 down-regulated mRNAs. Of these, FST, CCL3, CPE, RAPGEF4, IL6, MDFI, PDZD2, and GATM were primary hub genes (biomarkers) for osteoporosis. These differentially expressed genes were significantly enriched in GO terms related to extracellular matrix process and KEGG signaling pathways including osteoclast differentiation. In the functional experiments, the protein expression level of FST, CCL3, and RAPGEF4 displayed a specific expression pattern between osteoporosis patients and control group. The protein concentration of FST was 23.63 ± 6.39 ng/mL in osteoporosis patients compared as 48.36 ± 9.12 ng/mL in osteopenia group (P < 0.01). Meanwhile, CCL3 was 1.03 ± 0.64 ng/mL in osteoporosis patients vs 0.56 ± 0.24 in osteopenia group (P < 0.01) and RAPGEF4 was 53.58 ± 11.42 ng/mL in osteoporosis patients vs 66.47 ± 13.28 ng/mL in osteopenia group (P < 0.05), respectively.

Conclusion: This study has identified potential gene biomarkers (the genes with most significantly differential expression and useful for distinguishing osteoporosis from other bone disorders) and established a differential expression profile for osteoporosis, which is a valuable reference for future clinical research.
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http://dx.doi.org/10.1111/os.13094DOI Listing
September 2021

Biomarkers of inflammation and fibrosis in young adults with history of Kawasaki disease.

Int J Cardiol Heart Vasc 2021 Oct 1;36:100863. Epub 2021 Sep 1.

Dept. of Pediatrics, University of California San Diego, La Jolla, CA, United States.

Background: Myocardial histology from autopsies of young adults with giant coronary artery aneurysms following Kawasaki disease (KD) shows bridging fibrosis beyond the territories supplied by the aneurysmal arteries. The etiology of this fibrosis is unknown, but persistent, low-level myocardial inflammation and microcirculatory ischemia are both possible contributing factors. To investigate the possibility of subclinical myocardial inflammation or fibrosis, we measured validated biomarkers in young adults with a remote history of KD.

Methods: We measured plasma calprotectin, galectin-3 (Gal-3), growth differentiation factor-15 (GDF-15), soluble ST2 (sST2), and serum procollagen type 1C-terminal propeptide (P1CP) in 91 otherwise healthy young adults with a remote history of KD and in 88 age-similar, healthy controls. KD subjects were stratified by coronary artery aneurysm (CAA) status and history of remote myocardial infarction (MI).

Results: After correction for multiple testing, calprotectin, Gal-3, and GDF-15 levels were significantly higher in subjects with persistent CAA (n = 26) compared with KD subjects with remodeled CAA (n = 20, p = 0.005, 0.001, 0.0036, respectively). In a multivariable regression model with CA status as the main predictor and adjusting for sex, MI history, and interval from KD onset, CA status was a significant predictor (Persistent CAA vs KD Normal CA) of calprotectin, Gal-3, GDF-15 and sST2 levels (p = 0.004, <0.001, 0.007, and 0.049, respectively).

Conclusions: These results suggest that ongoing inflammation and fibrosis may be occurring in individuals with persistent CAA. Longitudinal follow-up is needed to clarify the clinical significance of these elevated biomarker levels in this patient population that requires life-long monitoring.
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http://dx.doi.org/10.1016/j.ijcha.2021.100863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413893PMC
October 2021

Bimetallic Mixed Clusters Highly Loaded on Porous 2D Graphdiyne for Hydrogen Energy Conversion.

Adv Sci (Weinh) 2021 Sep 8:e2102777. Epub 2021 Sep 8.

Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

There is no doubt that hydrogen energy can play significant role in promoting the development and progress of modern society. The utilization of hydrogen energy has developed rapidly, but it is far from the requirement of human. Therefore, it is very urgent to develop methodologies and technologies for efficient hydrogen production, especially high activity and durable electrocatalysts. Here a bimetallic oxide cluster on heterostructure of vanadium ruthenium oxides/graphdiyne (VRuO /GDY) is reported. The unique acetylene-rich structure of graphdiyne achieves outstanding characteristics of electrocatalyst: i) controlled preparation of catalysts for achieving multiple-metal clusters; ii) regulation of catalyst composition and morphology for synthesizing high-performance catalysts; iii) highly active and durable hydrogen evolution reaction (HER) properties. The optimal porous electrocatalyst (VRu O /GDY) can deliver 10 mA cm at low overpotentials of 13 and 12 mV together with robust long-term stability in alkaline and neutral media, respectively, which are much smaller than Pt/C. The results reveal that the synergism of different components can efficiently facilitate the electron/mass transport properties, reduce the energy barrier, and increase the active site number for high catalytic performances.
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http://dx.doi.org/10.1002/advs.202102777DOI Listing
September 2021

Triclosan weakens the nitrification process of activated sludge and increases the risk of the spread of antibiotic resistance genes.

J Hazard Mater 2021 08 25;416:126085. Epub 2021 May 25.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China. Electronic address:

The usage of triclosan (TCS) may rise rapidly due to the COVID-19 pandemic. TCS usually sinks in the activated sludge. However, the effects of TCS in activated sludge remain largely unknown. The changes in nitrogen cycles and the abundances of antibiotic resistance genes (ARGs) caused by TCS were investigated in this study. The addition of 1000 μg/L TCS significantly inhibited nitrification since the ammonia conversion rate and the abundance of nitrification functional genes decreased by 12.14%. The other nitrogen cycle genes involved in nitrogen fixation and denitrification were also suppressed. The microbial community shifted towards tolerance and degradation of phenols. The addition of 100 μg/L TCS remarkably increased the total abundance of ARGs and mobile genetic elements by 33.1%, and notably, the tetracycline and multidrug resistance genes increased by 54.75% and 103.42%, respectively. The co-occurrence network revealed that Flavobacterium might have played a key role in the spread of ARGs. The abundance of this genus increased 92-fold under the addition of 1000 μg/L TCS, indicating that Flavobacterium is potent in the tolerance and degradation of TCS. This work would help to better understand the effects of TCS in activated sludge and provide comprehensive insight into TCS management during the pandemic era.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126085DOI Listing
August 2021

Enhanced dechlorination of trichloroethene by sulfidated microscale zero-valent iron under low-frequency AC electromagnetic field.

J Hazard Mater 2021 Aug 24;423(Pt A):127020. Epub 2021 Aug 24.

Department of Earth and Planetary Sciences, University of, Tennessee, Knoxville, TN 37996, USA.

In this study an electromagnetic heating strategy is proposed for remediation of trichloroethene (TCE) by ball milled, sulfidated microscale zero valent iron (S-mZVI) particles. S-mZVI is ferromagnetic, which generates heat under the application of a low-frequency alternating current electromagnetic field (AC EMF). We found that the temperature reached up to ~120 ℃ during 30-min electromagnetic induction heating of 10 g/L S-mZVI (with S/Fe molar ratio of 0.1), compared with ~55 ℃ and ~80 ℃ for ZVI and ball milled mZVI, respectively. The application of AC EMF accelerated the TCE degradation rate (k = 5.5 × 10 h) by up to 4-fold without compromising or even enhancing electron efficiency of S-mZVI compared to no-heating. Furthermore, this process halved the generation of chlorinated intermediate, cis-DCE. In contrast, water-bath heating only increased the dechlorination rate 2-fold with unchanged cis-DCE generation and lowered electron efficiency. This is attributed to both rising temperature by induction heating and accelerated ZVI corrosion and surface Fe exposure caused by AC EMF. In real groundwater, the AC EMF maintained the same promoting effects for TCE dechlorination by S-mZVI. This study shows that combination of filed-scale available AC EMF with S-mZVI provides a promising approach for remediation of chlorinated hydrocarbons in contaminated groundwater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127020DOI Listing
August 2021

Citrus sinensis CBF1 Functions in Cold Tolerance by Modulating Putrescine Biosynthesis Through Regulation of ARGININE DECARBOXYLASE.

Plant Cell Physiol 2021 Sep 3. Epub 2021 Sep 3.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

C-repeat (CRT) binding factors (CBFs) are well known to act as crucial transcription factors that function in cold stress response. Arginine decarboxylase (ADC)-mediated putrescine biosynthesis has been reported to be activated in plants exposed to cold conditions, but it remains elusive whether CBFs can regulate ADC expression and putrescine accumulation. In this study, we show that cold up-regulated ADC gene (CsADC) and elevation of endogenous putrescine content in sweet orange (Citrus sinensis). Promoter of CsADC contains two CRT sequences that are canonical elements recognized by CBFs. Sweet orange genome contains four CBFs (CsCBF1-4), in which CsCBF1 was significantly induced by cold. CsCBF1, located in the nucleus, was demonstrated to bind directly and specifically to the promoter of CsADC and acted as a transcriptional activator. Overexpression of CsCBF1 led to notable elevation of CsADC and putrescine level in sweet orange transgenic plants, along with remarkably enhanced cold tolerance, relative to the wild type (WT). However, pretreatment with D-arginine, an ADC inhibitor, caused prominent reduction of endogenous putrescine level in the overexpressing lines, accompanied by greatly compromised cold tolerance. Taken together, these results demonstrate that CBF1 of sweet orange directly regulates ADC expression and modulates putrescine synthesis for orchestrating the cold tolerance. Our findings shed light into the transcriptional regulation of putrescine accumulation through targeting the ADC gene in the presence of cold stress. Meanwhile, this study illustrates a new mechanism underlying the CBF-mediated cold stress response.
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http://dx.doi.org/10.1093/pcp/pcab135DOI Listing
September 2021

Synthesis of New AIEE-Active Chalcones for Imaging of Mitochondria in Living Cells and Zebrafish In Vivo.

Int J Mol Sci 2021 Aug 19;22(16). Epub 2021 Aug 19.

School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China.

Fluorophores with aggregation-induced emission enhancement (AIEE) properties have attracted increasing interest in recent years. On the basis of our previous research, we successfully designed and synthesized eleven chalcones. Through an optical performance experiment, we confirmed that compounds - had obvious AIEE properties. As these AIEE molecules had excellent fluorescence properties and a large Stokes shift, we studied their application in living cell imaging, and the results showed that these compounds had low cytotoxicity and good biocompatibility at the experimental concentrations. More importantly, they could specifically label mitochondria. Subsequently, we selected zebrafish as experimental animals to explore the possibilities of these compounds in animal imaging. The fluorescence imaging of zebrafish showed that these AIEE molecules can enter the embryo and can be targeted to aggregate in the digestive tract, which provides a strong foundation for their practical application in the field of biological imaging. Compared with traditional fluorophores, these AIEE molecules have the advantages of possessing a small molecular weight and high flexibility. Therefore, they have excellent application prospects in the field of biological imaging. In addition, the findings of this study have very positive practical significance for the discovery of more AIEE molecules.
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http://dx.doi.org/10.3390/ijms22168949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396511PMC
August 2021

Sodium and Potassium Excretion of Schoolchildren and Relationship with Their Family Excretion in China.

Nutrients 2021 Aug 20;13(8). Epub 2021 Aug 20.

The George Institute for Global Health at Peking University Health Science Centre, Beijing 100600, China.

This cross-sectional study aimed to assess 24-h urinary sodium and potassium excretion in children and the relationships with their family excretion. Using the baseline data of a randomized trial conducted in three cities of China in 2018, a total of 590 children (mean age 8.6 ± 0.4 years) and 1180 adults (mean age 45.8 ± 12.9 years) from 592 families had one or two complete 24-h urine collections. The average sodium, potassium excretion and sodium-to-potassium molar ratio of children were 2180.9 ± 787.1 mg/d (equivalent to 5.5 ± 2.0 g/d of salt), 955.6 ± 310.1 mg/d and 4.2 ± 1.7 respectively, with 77.1% of the participants exceeding the sodium recommendation and 100% below the proposed potassium intake. In mixed models adjusting for confounders, every 1 mg/d increase in sodium excretion of adult family members was associated with a 0.11 mg/d (95% CI: 0.06 to 0.16, < 0.0001) increase in sodium excretion of children. The family-child regression coefficient corresponds to 0.20 mg/d (95% CI: 0.15 to 0.26, < 0.0001) per 1 mg/d in potassium and to 0.36 (95% CI: 0.26 to 0.45, < 0.0001) in sodium-to-potassium molar ratio. Children in China are consuming too much sodium and significantly inadequate potassium. The sodium, potassium excretion and sodium-to-potassium ratio of children are associated with their family excretions in small to moderate extent. Efforts are warranted to support salt reduction and potassium enhancement in children through comprehensive strategies engaging with families, schools and food environments.
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http://dx.doi.org/10.3390/nu13082864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402222PMC
August 2021

Investigation on Preparation and Properties of Crack Sealants Based on CNTs/SBS Composite-Modified Asphalt.

Materials (Basel) 2021 Aug 14;14(16). Epub 2021 Aug 14.

College of Transportation, Jilin University, Changchun 130025, China.

Crack is the main distress of asphalt pavement. Sealant is one of the most commonly used crack repair materials, and its performance is the key to affect the service life of asphalt pavements. In order to find an efficient modifier and optimize the performances of crack sealants. In this paper, carbon nanotubes (CNTs) and styrene-butadiene-styrene (SBS) were used as modifiers to prepare CNTs/SBS composite-modified asphalt crack sealant. The properties of the sealant were tested to evaluate its suitability for crack repair, which included the viscosity, softening point, resilience recovery, cone penetration, flow value, penetration, aging resistance, and fatigue resistance. The results showed that the conventional properties of the sealants meet the requirements of the specification. In addition, after heating aging, the elastic recovery rate of the sealant containing more CNTs decreased only slightly. The sealant containing 1 wt% CNTs exhibited a higher viscosity, fatigue resistance, thermal aging resistance.
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http://dx.doi.org/10.3390/ma14164569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401205PMC
August 2021

Study on sialic acid binding state in stewed bird's nest and optimization of enzymatic extraction of free and oligosaccharide-bound sialic acid.

J AOAC Int 2021 Aug 17. Epub 2021 Aug 17.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.

Background: As a traditional Chinese health food, edible bird's nest (EBN) has high medicinal value, which is mostly attributed to the high content of sialic acid (SA). SA mainly exists in protein-bound, oligosaccharide-bound and free forms and the binding forms of SA are closely related to the functions of EBN.

Objective: To establish a simple but robust method to distinguish and determinate the free and oligosaccharide-bound SA content and the protein-bound SA content, and investigate the changes of SA binding state in EBN during different processing.

Methods: Protein-bound SA in EBN was separated from other forms of SA by trichloroacetic acid precipitation, and SA content was determined by high performance liquid chromatography (HPLC). The effects of stewing conditions on the distribution of SA in EBN were investigated and response surface methodology was used to explore the optimal conditions for enzymatic extraction of free and oligosaccharide-bound SA from EBN.

Results: The average recoveries of free and oligosaccharide-bound SA and protein-bound SA were 97.82%-98.92% and 94.67%-95.75%. The content of free and oligosaccharide-bound SA in stewed EBN was proportional to the stewing temperature, stewing time and liquid to material ratio, while that of protein-bound SA was inversely proportional to those factors. Through response surface analysis, we found that the optimum technological parameters were as follows: liquid to material ratio was 60: 1, enzymolysis time was 2 h, enzyme dosage was 12000 U/g (alkaline protease), pH was 11, enzymolysis temperature was 60 °C.

Conclusion: This method can not only distinguish free and oligosaccharide-bound SA and protein-bound SA effectively, but also determine the contents of them. The results of investigation on stewing conditions and response surface analysis can be used as the theoretical basis for the further pharmacological research of EBN, and can also provide theoretical guidance for the development of EBN products.

Highlights: A method for the determination of free and oligosaccharide-bound SA and protein-bound SA in EBN by HPLC was established, and the extraction process of free and oligosaccharide-bound SA was optimized.
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http://dx.doi.org/10.1093/jaoacint/qsab096DOI Listing
August 2021

Synthesis of trans- methyl ferulate bearing an oxadiazole ether as potential activators for controlling plant virus.

Bioorg Chem 2021 Oct 8;115:105248. Epub 2021 Aug 8.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China. Electronic address:

A series of new ferulic acid derivatives bearing an oxadiazole ether was synthesized by introducing a structure of oxadiazole into trans-ferulic acid via an ether linkage. The synthesized target compounds were evaluated in vivo for their anti-TMV (tobacco mosaic virus) activity, which indicated that some synthesized compounds displayed strong activity for controlling TMV. For protective activity, compounds 6f and 6h had the most activities of 65% and 69.8% at 500 mg L, respectively. Compounds 6a, 6b, 6e, 6f and 6h showed > 60% curative activities at 500 mg L. Preliminary proteomics analysis showed that compound 6h could regulate the phenylpropanoid biosynthesis pathway and chloroplast function. These results indicated that synthesized novel ferulic acid derivatives could be used for controlling TMV.
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http://dx.doi.org/10.1016/j.bioorg.2021.105248DOI Listing
October 2021

Dopaminergic Projection from Ventral Tegmental Area to Substantia Nigra Pars Reticulata Mediates Chronic Social Defeat Stress-Induced Hypolocomotion.

Mol Neurobiol 2021 Aug 12. Epub 2021 Aug 12.

Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, People's Republic of China.

Numerous human clinical studies have suggested that decreased locomotor activity is a common symptom of major depressive disorder (MDD), as well as other psychiatric diseases. In MDD, the midbrain ventral tegmental area (VTA) dopamine (DA) neurons are closely related to regulate the information processing of reward, motivation, cognition, and aversion. However, the neural circuit mechanism that underlie the relationship between VTA-DA neurons and MDD-related motor impairments, especially hypolocomotion, is still largely unknown. Herein, we investigate how the VTA-DA neurons contribute to the hypolocomotion performance in chronic social defeat stress (CSDS), a mouse model of depression-relevant neurobehavioral states. The results show that CSDS could affect the spontaneous locomotor activity of mice, but not the grip strength and forced locomotor ability. Chemogenetic activation of VTA-DA neurons alleviated CSDS-induced hypolocomotion. Subsequently, quantitative whole-brain mapping revealed decreased projections from VTA-DA neurons to substantia nigra pars reticulata (SNr) after CSDS treatment. Optogenetic activation of dopaminergic projection from VTA to SNr with the stimulation of phasic firing, but not tonic firing, could significantly increase the locomotor activity of mice. Moreover, chemogenetic activation of VTA-SNr dopaminergic circuit in CSDS mice could also rescued the decline of locomotor activity. Taken together, our data suggest that the VTA-SNr dopaminergic projection mediates CSDS-induced hypolocomotion, which provides a theoretical basis and potential therapeutic target for MDD.
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http://dx.doi.org/10.1007/s12035-021-02522-7DOI Listing
August 2021

Primary intracranial papillary meningioma: Analysis of factors of prognosis and systematic review.

J Clin Neurosci 2021 Sep 5;91:118-124. Epub 2021 Jul 5.

Department of Neurosurgery, Jining NO.1 People's Hospital, Affiliated Jining NO.1 People's Hospital of Jining Medical University, Jining Medical University, Jining 272011, Shandong, China. Electronic address:

Objective: Papillary meningioma is rare and displays an aggressive clinical behavior with poor prognosis. Therefore, we performed an extensive literature review to evaluate the adverse factors and treatment strategy of survival.

Method: We performed Ovid, Medline, Embase, Pubmed, Web of Science and Cochrane database queries for articles published between 1938 and 2019 with the search term "WHO grade III meningioma" or "papillary meningioma" and "central nervous system", "cerebral", or "intracranial".

Results: After a careful evaluation, a total of 19 studies were included. The entire cohort included the 67 patients, 34 (50.7%) were male and 33 (49.3%) were female with a mean age of 32.6 ± 2.1 years ranging from 4.5 months to 74 years. Gross total resection was achieved in 48 (71.6%) cases, and 29 (51.8%) patients received postoperative radiation. The mean follow-up period was 42.3 ± 4.4 months (range, 2-197 months). Thirty-six (53.7%) patients happened to recurrences, 11 (16.4%) patients happened to extracranial metastasis and 25 (37.3%) patients died. Univariate analysis revealed that the MIB > 5% trended toward a shorter time to recurrence (p = 0.084). Gross total resection was associated with favorable progression-free survival (p = 0.007) and overall survival (p = 0.001). Postoperative radiation was associated with favorable progression-free survival (p = 0.001).

Conclusions: Gross total resection and adjuvant radiation were recommended as the initial treatment option for patients with papillary meningioma.
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http://dx.doi.org/10.1016/j.jocn.2021.06.025DOI Listing
September 2021

Changes in N6-Methyladenosine Modification Modulate Diabetic Cardiomyopathy by Reducing Myocardial Fibrosis and Myocyte Hypertrophy.

Front Cell Dev Biol 2021 21;9:702579. Epub 2021 Jul 21.

Graduate School, Peking Union Medical College, Beijing, China.

In this study, we aimed to systematically profile global RNA N6-methyladenosine (mA) modification patterns in a mouse model of diabetic cardiomyopathy (DCM). Patterns of mA in DCM and normal hearts were analyzed via mA-specific methylated RNA immunoprecipitation followed by high-throughput sequencing (MeRIP-seq) and RNA sequencing (RNA-seq). mA-related mRNAs were validated by quantitative real-time PCR analysis of input and mA immunoprecipitated RNA samples from DCM and normal hearts. A total of 973 new mA peaks were detected in DCM samples and 984 differentially methylated sites were selected for further study, including 295 hypermethylated and 689 hypomethylated mA sites (fold change (FC) > 1.5, < 0.05). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses indicated that unique mA-modified transcripts in DCM were closely linked to cardiac fibrosis, myocardial hypertrophy, and myocardial energy metabolism. Total mA levels were higher in DCM, while levels of the fat mass and obesity-associated (FTO) protein were downregulated. Overexpression of FTO in DCM model mice improved cardiac function by reducing myocardial fibrosis and myocyte hypertrophy. Overall, mA modification patterns were altered in DCM, and modification of epitranscriptomic processes, such as mA, is a potentially interesting therapeutic approach.
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http://dx.doi.org/10.3389/fcell.2021.702579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8334868PMC
July 2021

High Voltage-Stabilized Graphdiyne Cathode Interface.

Small 2021 Sep 8;17(38):e2102066. Epub 2021 Aug 8.

Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Research/Education Center for Excellence in Molecular Sciences Institute of Chemistry, Chinese Academy of Science, Beijing, 100190, P. R. China.

Suppressing the irreversible interfacial reactions is an important scientific bottleneck in the development of stable high-energy-density lithium-ion battery. The interfacial chemistry of graphdiyne (GDY) on the high-voltage cathode of LiNi Mn O (LNMO) shows a very interesting process, in which the sp-hybridization carbon atoms chemically scavenge the hydrofluoric acid (HF) and in situ form the fluorinated GDY interface. It first turns the harmful HF into profit, and greatly enhances the interfacial stability and restrains the side reaction on the cathode under high working voltage. The GDY-coated LNMO cathode obviously alleviates the electrolyte degradation, achieves high Coulombic efficiency and reliability. Due to atomic-level selectivity and chemical trapping of HF by GDY, it effectively suppresses the dissolution of Mn, Ni elements. These results highlight the unparalleled advantages of GDY in the formation of high stable interfaces and protection of high-energy-density electrodes.
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http://dx.doi.org/10.1002/smll.202102066DOI Listing
September 2021

Barriers, Enablers, and Perceptions on Dietary Salt Reduction in the Out-of-Home Sectors: A Scoping Review.

Int J Environ Res Public Health 2021 07 30;18(15). Epub 2021 Jul 30.

Wolfson Institute of Preventive Medicine, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK.

In this review, we have investigated the perceptions, barriers, and enabling factors that were responsible for a dietary salt reduction in the out-of-home sectors. For this purpose, we examined different knowledge databases such as Google Scholar, Ebscohost, MEDLINE (PubMed), Ovid, and Cochrane Library for research articles from September to December 2020. The inclusion criteria for the research articles were that they had to be published in English and had to be a qualitative or quantitative study that was conducted after 2010. These studies also had to report the various enablers, barriers, and perceptions regarding salt reduction in the out-of-home sectors. After implementing the inclusion criteria, we successfully screened a total of 440 studies, out of which 65 articles fulfilled all the criteria. The perceived barriers that hindered salt reduction among the out-of-home sectors included lack of menu and food variabilities, loss of sales due to salt reduction, lack of technical skills for implementing the salt reduction processes for cooking or reformulation, and an absence of environmental and systemic support for reducing the salt concentration. Furthermore, the enablers for salt reduction included the intervention programs, easy accessibility to salt substitutes, salt intake measurement, educational availability, and a gradual reduction in the salt levels. With regards to the behavior or perceptions, the effect of organizational and individual characteristics on their salt intake were reported. The majority of the people were not aware of their salt intake or the effect of salt on their health. These people also believed that low salt food was recognized as tasteless. In conclusion, the enablers, barriers, and perceptions regarding salt reduction in the out-of-home sectors were multifaceted. Therefore, for the implementation of the strategies, policies, and initiatives for addressing the barriers, the policymakers need to encourage a multisectoral collaboration for reducing the salt intake in the population.
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http://dx.doi.org/10.3390/ijerph18158099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345971PMC
July 2021

HMGB2 promotes chondrocyte proliferation under negative pressure through the phosphorylation of AKT.

Biochim Biophys Acta Mol Cell Res 2021 Oct 30;1868(11):119115. Epub 2021 Jul 30.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Department of Oral Anatomy and Physiology and TMD, School of Stomatology, the Fourth Military Medical University, Xi'an, China. Electronic address:

Cells in articular cartilage are zonal arranged. Cells in superficial zone cartilage are generally small and proliferative. Appropriate negative pressure stimulation is beneficial to cell survival and tissue repair. Whether negative pressure has promotive impact on the proliferation activity of the superficial zone chondrocytes is of interest. In this study, we isolated superficial chondrocytes from the mandibular condylar cartilage of rats. After negative pressure treatment, the cells were collected for RNA-sequencing, quantitative real-time PCR and western blotting assays, aiming to detect the proliferative responses of chondrocytes to negative pressure and explore the potential molecular mechanisms. Data from RNA-sequencing analysis indicated that the superficial chondrocytes responded to the 4 h -10 kPa treatment by a significant increase in proliferation. In addition, the expression of high-mobility group box 2 (HMGB2) and the phosphorylation of AKT were obviously promoted. Knockdown of HMGB2 decreased AKT phosphorylation and diminished the negative pressure-induced proliferation of chondrocytes, as shown by decreased expression of Ki67 and cyclin-dependent kinase 6 (CDK6). In contrast, overexpression of HMGB2 enhanced AKT phosphorylation and further promoted proliferative activity. Moreover, LY294002, an AKT inhibitor, suppressed the proliferative activity of chondrocytes under negative pressure, while SC79, an activator of AKT phosphorylation, enhanced the proliferation of chondrocytes. Our data demonstrated that HMGB2 exhibits a promotion impact on chondrocyte proliferation under negative pressure via the phosphorylation of AKT. These results provide a new perspective for superficial zone chondrocytes proliferation under negative pressure, which should be benefit for cartilage regeneration.
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http://dx.doi.org/10.1016/j.bbamcr.2021.119115DOI Listing
October 2021

[Research progress of necroptosis on bone related diseases].

Zhongguo Gu Shang 2021 Jul;34(7):687-90

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Department of Oral Anatomy and Physiology and TMD, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, Shaanxi, China.

As a new type of cell death, necroptosis is initiated by tumor necrosis factor receptor 1(TNFR1), and then activated receptor-interacting protein kinase 1(RIP1) and receptor-interacting protein kinase 3 (RIP3), following by the activation of mixed lineage kinase domain-like protein(MLKL) to deliver cell death signal. When necroptosis happens, damage associated molecular patterns (DAMPs) enter into extracellular area through the ruptured cytomembrane, followed by the disordered tissue hemeostasis. In recent years, many researches showed that necroptosis playimportant roles in a few bone related diseases, such as osteoporosis, osteonecrosis, osteosarcoma, etc. Thus, we try to briefly review the researches in this field.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.07.020DOI Listing
July 2021

17.6%-Efficient Quasiplanar Heterojunction Organic Solar Cells from a Chlorinated 3D Network Acceptor.

Adv Mater 2021 Sep 28;33(37):e2102778. Epub 2021 Jul 28.

Shenzhen Grubbs Institute and Department of Chemistry, Southern University of Science and Technology, Shenzhen, 518055, China.

Bulk heterojunction (BHJ) organic solar cells (OSCs) have achieved great success because they overcome the shortcomings of short exciton diffusion distances. With the progress in material innovation and device technology, the efficiency of BHJ devices is continually being improved. For some special photovoltaic material systems, it is difficult to manipulate the miscibility and morphology of blend films, and this results in moderate, even poor device performance. Quasiplanar heterojunction (Q-PHJ) OSCs have been proposed to exploit the excellent photovoltaic properties of these materials. An OSC with BTIC-BO-4Cl has a 3D interpenetrating network structure with multiple channels that can facilitate the exciton diffusion and charge transport, and BTIC-BO-4Cl is therefore a good candidate for Q-PHJ OSCs. In this work, a D18:BTIC-BO-4Cl-based Q-PHJ device is fabricated. The exciton diffusion lengths of D18 and BTIC-BO-4Cl are in accord with the requirements of the Q-PHJ device and the efficiency of Q-PHJ device is as high as 17.60%. This study indicates that the Q-PHJ architecture can replace the BHJ architecture to produce excellent OSCs for certain unique donors and acceptors, providing an alternative approach to photovoltaic material design and device fabrication.
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http://dx.doi.org/10.1002/adma.202102778DOI Listing
September 2021
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