Publications by authors named "Feng Hao"

696 Publications

Liver metastasis as the initial clinical manifestation of sublingual gland adenoid cystic carcinoma: A case report.

World J Clin Cases 2021 Jul;9(19):5238-5244

Department of Radiology, The First People's Hospital of Zigong, Zigong 643099, Sichuan Province, China.

Background: Adenoid cystic carcinoma (ACC) is a common malignant tumor of salivary gland. The lung and liver are frequent sites of distant metastasis. Liver metastasis as the initial clinical manifestation of sublingual gland ACC is very rare.

Case Summary: A 51-year-old Chinese woman presented with a painless mass in the right lobe of liver. The tumor was composed of ductal cells and myoepithelial cells with a morphology including tubiform and cribriform structures. Immunostaining results showed ductal cells positive for CK7, CK14, CK19, CD117, and 34βE12, and negative for MYB, vimentin, ER, PR, and CEA. The myoepithelial cells were positive for p63, calponin and CK5/6. Metastatic salivary ACC was considered, and a sublingual gland mass was revealed by computed tomography. Histological evaluation confirmed primary sublingual gland ACC. Fluorescence hybridization (FISH) did not find an MYB-NFIB fusion gene in specimens from either the primary or metastatic ACC tumors. The sublingual gland ACC relapsed in 20 mo. The recurrent lesion disappeared following local radiation therapy and computed tomography-guided radioactive seed implantation. The patient remains in good condition until now.

Conclusion: Metastatic sublingual gland ACC with initial clinical manifestation as a liver mass is very rare, and was pathologically confirmed in this patient by its histological appearance. Primary hepatic tumors and metastatic carcinomas should be included in the differential diagnosis. Immunohistochemical detection of MYB protein and MYB-NFIB fusion gene detection by FISH can be helpful, but occasional negative results confuse the diagnosis.
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http://dx.doi.org/10.12998/wjcc.v9.i19.5238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283605PMC
July 2021

Enhanced activity promoted by amorphous metal oxyhydroxides on CeO for alkaline oxygen evolution reaction.

J Colloid Interface Sci 2021 Jul 7;604:719-726. Epub 2021 Jul 7.

College of Materials Science and Engineering, Sichuan University, Chengdu 610064, Sichuan, China. Electronic address:

Herein, we demonstrate a direct growth of amorphous metal oxyhydroxide (AMO) attached on CeO by a galvanic replacement mechanism as advanced oxygen evolution reaction (OER) catalyst. In this unique structure, the CeO substrate not only offers high specific surface area for the formation of AMO, but also provides high conductivity, guaranteeing the promoted electron transfer for the catalytic reaction. In addition, the AMO on the surface of the CeO exposes abundant active sites for the OER. Benefiting from the above advantages, the as-prepared [email protected] supported on nickel foam ([email protected]/NF) exhibits excellent OER performance with low overpotential of 261 mV at 10 mA cm, high turnover frequency of 0.07 s at 20 mA cm and superior stability in 1.0 M KOH.
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http://dx.doi.org/10.1016/j.jcis.2021.06.149DOI Listing
July 2021

Sevoflurane exerts improved protective effects than propofol on hypoxia-reoxygenation injury by regulating the microRNA-221-5p/ADAM8 axis in cardiomyocytes.

Exp Ther Med 2021 Aug 18;22(2):893. Epub 2021 Jun 18.

Department of Neurosurgery, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Myocardial ischemia-reperfusion (I/R) injury is a leading cause of heart disease and death. Decreasing the detrimental effect of I/R remains an urgent issue in clinical practice. The present study examined the interaction of the anesthetics (sevoflurane and propofol), ADAM8, and microRNA (miR)-221-5p in myocardial tissue protection in the hypoxia-reoxygenation (H/R) model. H9C2 cells were cultured and subjected to H/R stimulation for further verifications . Reverse transcription-quantitative PCR or western blotting was performed to evaluate mRNA or protein expression levels. Cell Counting Kit-8, BrdU, and caspase-3 activity assays were performed to investigate cell viability, proliferation and apoptosis. A dual-luciferase reporter assay was performed to verify the association between miR-221-5p and ADAM8. Sevoflurane had greater protective effects on the life of cardiomyocytes with H/R injury compared with propofol by promoting cell viability, proliferation and inhibiting apoptosis. Concurrently, compared with propofol-treated H/R injured cardiomyocytes, the expression level of ADAM8 in sevoflurane-treated H/R injured cardiomyocytes was higher. In addition, overexpression of ADAM8 promoted the cell viability and proliferation of sevoflurane-treated cardiomyocytes with H/R injury but inhibited cell apoptosis, while the downregulation of miR-221-5p showed an opposite trend to that of ADAM8 overexpression. The present data provide evidence that sevoflurane can mediate the miR-221-5p/ADAM8 axis, playing a better protective role compared with propofol in cardiomyocytes with H/R injury.
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http://dx.doi.org/10.3892/etm.2021.10325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243314PMC
August 2021

Effects of different plastic film mulching on soil hydrothermal conditions and grain-filling process in an arid irrigation district.

Sci Total Environ 2021 Jul 5;795:148886. Epub 2021 Jul 5.

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest A&F University, Yangling 712100, China; College of Water Resources and Architectural Engineering, Northwest A&F University, Yangling 712100, Shaanxi Province, China; Institute of Water-saving Agriculture in Arid Areas of China, Northwest A&F University, Yangling 712100, China; Institute of Soil and Water Conservation, Northwest A & F University, Yangling 712100, China. Electronic address:

Plastic film mulching has been extensively used for spring maize (Zea mays L.) production in the Hetao Irrigation District (HID). Determining whether transparent plastic film mulching results in premature senescence and yield reduction of spring maize still needs to be verified. A two-year field experiment was conducted in the HID that involved planting spring maize under three mulching practices on a flat plot 1) without mulching (control treatment, CK), 2) with black plastic film mulching (BM), 3) with transparent plastic film mulching (TM). The results indicated that TM and BM were superior to CK in terms of effects on soil hydrothermal conditions. Compared with BM, TM produced significantly higher soil temperature at V6, and had no significant temperature effect at V12, R1, R3, and R6. Both TM and BM promoted early seedling emergence and earlier silking, and TM extended the duration of the reproductive stages by 1-2 days compared with BM, and 4-5 days longer than CK. TM and BM produced greater kernel weights and kernel volumes in the superior and middle portions of the ear than CK. TM produced significantly greater total kernel weights per ear than BM at and after 23 days after silking. TM significantly increased grain-filling rate and length of the active grain-filling period compared with BM and CK. Additionally, TM and BM produced significantly higher photosynthetic parameters than CK at the grain-filling stage in the two study years. The net photosynthesis rate for TM was significantly greater than for BM. TM and BM significantly increased grain yields by 28.1% and 15.1%, respectively, in 2019 over CK, and by 24.6% and 21.1% in 2020. Transparent plastic film mulching could serve as a promising adaptive management practice to increase resource use efficiency and to improve maize productivity in the HID.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148886DOI Listing
July 2021

Long Non-coding RNA LINC01119 Promotes Neuropathic Pain by Stabilizing BDNF Transcript.

Front Mol Neurosci 2021 21;14:673669. Epub 2021 Jun 21.

Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Neuropathic pain (NP) is caused by primary injury or dysfunction of the peripheral and the central nervous system. Long non-coding RNAs were critical regulators involved in nervous system diseases, however, the precise regulatory mechanism remains unclear. This study aims to uncover the essential role of LINC01119 in NP progression and further clarify the underlying regulatory mechanism at post-transcriptional level. LINC01119 was significantly upregulated in rats of spare nerve injury (SNI) group compared to sham group. Functionally, silencing of LINC01119 significantly alleviated the neuropathic pain-induced hypersensitivity and reduced the increase in IL-6, IL-1β, and TNF-α caused by SNI. Mechanistically, Brain-derived neurotrophic factor (BDNF) was identified as the functional target of LINC01119. Besides, an RNA binding protein, ELAVL1 could directly interact with LINC01119, and this formed LINC01119- ELAVL1 complex binds to BDNF mRNA, strengthening its RNA stability and increasing the expression level of BDNF at both transcript and protein levels. Clinically, serum LINC01119 was verified as a promising diagnostic biomarker for NP patients. LINC01119 induces NP progression via binding with ELAVL1 and increasing BDNF mRNA stability and expression level. Therefore, LINC01119 may serve as a promising diagnostic marker and therapeutic target for NP treatment.
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http://dx.doi.org/10.3389/fnmol.2021.673669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255623PMC
June 2021

Carbon footprint of a winter wheat-summer maize cropping system under straw and plastic film mulching in the Loess Plateau of China.

Sci Total Environ 2021 Jun 23;794:148590. Epub 2021 Jun 23.

College of Water Resources and Architectural Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

The objective of this study was to calculate the carbon footprint (CF) of straw and plastic film mulching practices in order to identify the optimum field management for low-carbon agriculture. A four-year field experiment was conducted to determine the effects of different mulching measurements on greenhouse gas (GHG) emissions, grain yield, and CF of a winter wheat-summer maize cropping system in the Loess Plateau of China. Mulching treatments were no mulching (NM), straw mulching (SM), half plastic film mulching (HPM); full plastic film mulching (FPM), and ridge-furrow planting with film mulching over ridges (RPM). Plastic film mulching decreased NO emissions compared with NM. However, SM significantly increased direct NO emissions by 59.2% and indirect NO emissions by 16.2%. Average annual total GHG emissions calculated by life cycle assessment were 5199-7631 kg CO-eq ha yr. Nitrogen (N) fertilizer was the largest contributor to total GHG emissions, accounting for >41%. For plastic film mulching treatments, the second greatest contributor was plastic film, accounting for 21.1-35.7% of total GHG emissions. In contrast, the second greatest contributor was direct and indirect NO and CH emissions under NM (17.2%) and SM (21.6%). Emissions from diesel consumption was the third largest component of total GHG emissions. All mulching treatments showed significantly greater annual grain yield than the NM treatment. The CF of summer maize yield was higher than that of winter wheat. SM showed the lowest CF (0.38 kg CO-eq kg), and plastic film mulching increased CFs compared with NM. These results suggest that SM should be the priority mulching practice used to increase yield and to reduce the CF of winter wheat-summer maize production in the Loess Plateau, China. Optimizing N fertilizer application rates should be one of the key production strategies employed to mitigate agricultural GHG emissions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148590DOI Listing
June 2021

Lipid Levels and 3-Month Prognosis After Spontaneous Intracerebral Hemorrhage in Women.

Front Neurol 2021 17;12:690194. Epub 2021 Jun 17.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

The relationship between serum lipids levels and prognosis after spontaneous intracerebral hemorrhage (ICH) is still unclear. We aim to examine the association between lipid levels and 3-month ICH prognosis in women. We went through a registry of spontaneous ICH cases and selected female patients to study according to our criteria. We collected demographic, clinical, and laboratory information and evaluated serum triglyceride (TG) levels, total cholesterol (TC) levels, low-density cholesterol (LDLC) levels, high-density cholesterol (HDLC) levels, non-high-density cholesterol (non-HDLC) levels, and 3-month modified Rankin Scale (mRS). Multivariate logistic regression was performed, and receiver operating characteristic (ROC) curves were plotted to explore the relationship between serum lipid levels and 3-month ICH clinical outcomes. Two hundred six female patients were included in this study, and 96 (46.6%) of them had poor functional outcomes. In the univariate analysis, low TG ( = 0.006), TC ( = 0.025), LDLC ( = 0.001), non-HDLC ( < 0.001) levels, and high HDL ( = 0.036) levels were associated with poor 3-month clinical outcomes in women. In the multivariate logistic regression, low levels of TG (OR = 0.711, 95% CI = 0.542-0.933, = 0.014), TC (OR = 0.523, 95% CI = 0.304-0.903, = 0.020), LDLC (OR = 0.538, 95% CI = 0.307-0.942, = 0.030), non-HDLC (OR = 0.327, 95% CI = 0.177-0.603, < 0.001), and a high level of HDLC (OR = 2.075, 95% CI = 1.064-4.047, = 0.032) with area under the curve (AUC) of 0.610, 0.590, 0.630, 0.645, and 0.415, respectively, remained as independent indicators of poor prognosis at 3 months after adjusting for confounding factors. Low levels of TG, TC, LDLC, non-HDLC, and high levels of HDLC were independently associated with poor prognosis of spontaneous ICH in women.
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http://dx.doi.org/10.3389/fneur.2021.690194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247568PMC
June 2021

Quantifying the interaction of water and radiation use efficiency under plastic film mulch in winter wheat.

Sci Total Environ 2021 Jun 25;794:148704. Epub 2021 Jun 25.

Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA.

Local natural resources, (e.g., precipitation, solar radiation) are important for developing environmentally and scientifically sound management practices in dryland agroecosystem. Maximizing water use efficiency (WUE) in dryland farming systems remains a challenge. The objectives of this study were to assessing the robustness of radiation use efficiency (RUE) during different periods and investigate the interaction between RUE and WUE from water loss pattern and canopy development during wheat growth under different agricultural practices (non-mulched control, CK; transparent film mulching, TF; and black film mulching, BF) from 2013 to 2016 on the Loess Plateau, Northwest China. Results showed that RUE was mainly improved during post-anthesis under PM treatments. PM treatments contributed to elevated canopy photosynthesis and a delayed RUE peak during the reproductive period. Due to the increased spike number and ratio of plant transpiration to soil evaporation, TF and BF treatments had relatively stable photosynthetic activity relative to the CK treatment even those during dry periods. Initially, no relationship was found between WUE and RUE under the CK treatment. On the other hand, RUE and WUE were positively related in TF and BF treatments following a power function. RUE values increased with WUE rapidly to stabilize at a plateau value of 5.5 g MJ under TF and BF treatments, and thus, the wheat WUE had a higher improvement potential than RUE as it did not have an apparent plateau value. PM treatments enhanced the wheat production by taking full advantage of local solar radiation and precipitation (improving RUE and WUE). This higher use efficiency of resources produced more photoassimilates for wheat than that under the CK management, increased source size (LAI) and sink size (spike number) during wheat growth seasons, and thus increased the final grain yield.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148704DOI Listing
June 2021

Biochemical and functional characterization of two microbial type terpene synthases from moss Stereodon subimponens.

Plant Physiol Biochem 2021 Jun 25;166:750-760. Epub 2021 Jun 25.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. Electronic address:

Terpenes constitute a large class of plant secondary metabolites. Usually, there is only one type of terpene synthase in seed plants, which is called typical plant terpene synthase. Currently, as a new family of plant terpene synthases, microbial terpene synthase-like (MTPSL) is identificated in nonseed plants. However, our knowledge about the biological function of most MTPSLs in nonseed plants is very limited. Here, we investigated the biochemical and functional characterization of the enzymes encoded by two MTPSLs from moss Stereodon subimponens, SsMTPSL1 and SsMTPSL2. A phylogenetic tree analysis showed that SsMTPSL1 and SsMTPSL2 are homologous to AaMTPSL1, AaMTPSL3, ApMTPSL1, and ApMTPSL3 from hornworts. The enzyme activity experiment demonstrated that SsMTPSL1 has monoterpene synthase and sesquiterpene synthase activity, and SsMTPSL2 has monoterpene synthase activity. Next, we selected SsMTPSL1 to study its biochemical functions. Anti-bacterial activity test in vitro showed that the products of SsMTPSL1 have an anti-bacterial effect on Escherichia coli, Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000), and Staphylococcus aureus. To further understand the function of SsMTPSL1, the transgenic Arabidopsis thaliana plant of SsMTPSL1 is inoculated by Pst DC3000, and the result showed that SsMTPSL1 enhances the resistance of A. thaliana to Pst DC3000. All in all, this study provides new information about the functions of moss MTPSLs.
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http://dx.doi.org/10.1016/j.plaphy.2021.06.047DOI Listing
June 2021

Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple.

Hortic Res 2020 Jul 1;7(1):102. Epub 2020 Jul 1.

State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.

MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress. In this study, using a metabolomic approach, we identified differentially accumulated phenylpropanoid and flavonoid metabolites in MdMYB88/124 transgenic RNAi plants under control and long-term drought stress conditions in apple roots. We confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought conditions. Using Electrophoretic Mobility Shift Assay (EMSA) and ChIP-quantitative PCR (qPCR) analyses, we found that MdMYB88 positively regulates the MdCM2 gene, which is responsible for phenylalanine biosynthesis, through binding to its promoter region. Under long-term drought conditions, MdMYB88/124 RNAi plants consistently accumulated increased amounts of HO and MDA, while MdMYB88 and MdMYB124 overexpression plants accumulated decreased amounts of HO and MDA. We also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of MdMYB88 and MdMYB124 transgenic apple plants after long-term drought stress. We found that metabolites responsible for plant defense, including phenylpropanoids and flavonoids, accumulated less in the RNAi plants but more in the overexpression plants under both control and drought conditions. We further demonstrated that MdMYB88/124 RNAi plants were more sensitive to Alternaria alternata f. sp. mali and Valsa mali, two pathogens that currently severely threaten apple production. In contrast, MdMYB88 and MdMYB124 overexpression plants were more tolerant to these pathogens. The cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124, further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance, and provided information concerning molecular aspects of their roles in disease resistance.
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http://dx.doi.org/10.1038/s41438-020-0324-2DOI Listing
July 2020

Light-induced activities of novel naphtho[1,8-ef]isoindole-7,8,10(9H)-trione and oxoisoaporphine derivatives towards mosquito larvae.

Bioorg Med Chem Lett 2021 Jun 24;48:128225. Epub 2021 Jun 24.

Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Infected mosquitoes are significant vectors of dengue, yellow fever, chikungunya, zika and other pathogens. In the view of increasing resistance in mosquito larvae control, photoactivated insecticides is a promising approach by utilizing highly toxic singlet oxygen produced by photosensitizer through irradiation. However, the choice of photosensitizer for mosquito control is limited. Here, we report a novel series of naphtho[1,8-ef]isoindole-7,8,10(9H)-trione and oxoisoaporphines derivatives as excellent type II photosensitizers. Meanwhile, the light-dependent activities against permethrin-susceptible and permethrin-resistant strain of Aedes aegypti mosquito larvae of these compounds were evaluated. Among them, compound 7b was proved to be potential photodynamic insecticide due to its excellent phototoxicity, the LC value was 0.19 μg mL under visible light irradiation. The irradiation-generated enhancement in the activity was more than 520-fold. This compound could be the potential candidate in the search for new photoactivated insecticide leads. Importantly, 7b has good fluorescence quantum yield (ϕ = 0.70), it can be used as a fluorescence indicator in mosquito larvae to observe uptake and morphology change.
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http://dx.doi.org/10.1016/j.bmcl.2021.128225DOI Listing
June 2021

Electrocatalytic generation and tuning of ultra-stable and ultra-dense nanometre bubbles: an molecular dynamics study.

Nanoscale 2021 Jul;13(25):11242-11249

MIIT Key Laboratory of Thermal Control of Electronic Equipment, School of Energy and Power Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

Electrocatalytic generation of nanometre gas bubbles (nanobubbles) and their tuning are important for many energy and chemical processes. Studies have sought to use indirect or ex situ methods to investigate the dynamics and properties of nanobubbles, which are of fundamental interest. Alternatively, we present a molecular dynamics simulation method, which features in situ and high spatial resolution, to directly address these fundamentals. Particularly, our simulations can quantitatively reproduce the generation of ultra-stable and ultra-dense nanobubbles observed in electrochemical experiments. More importantly, our results demonstrate that the classical nucleation theory is still valid even for the scale down to several nanometres, to predict the dynamics and properties of nanobubbles. This provides general guidelines to design efficient nanocatalysts and nanoelectrodes. In our specific case, nanoelectrodes with wetting angles below 71° can suppress the generation of surface nanobubbles.
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http://dx.doi.org/10.1039/d1nr01588eDOI Listing
July 2021

RIPK3 collaborates with RIPK1 to inhibit MAVS-mediated signaling during black carp antiviral innate immunity.

Fish Shellfish Immunol 2021 Aug 17;115:142-149. Epub 2021 Jun 17.

State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China. Electronic address:

Both the activation and attenuation of MAVS/IFN signaling are critical for host defensing against viral infection and thus lead to an elaborate regulation of MAVS-mediated signaling. However, the regulatory mechanisms concerning MAVS/IFN signaling in teleost fish are not well understood. RIPK3 has been identified as a key regulator of necroptosis, apoptosis, and inflammatory signaling in human and mammals. Here we report the identification of the RIPK3 homologue from black carp Mylopharyngodon piceus (bcRIPK3) and describe its role in regulating MAVS/IFN signaling. qPCR results demonstrated that bcRIPK3 was transcriptionally activated in response to poly (I:C) or LPS stimulation. Immunoblot assay and immunofluorescent staining assay showed that bcRIPK3 was a cytosolic protein with molecular weights of 47 kDa. Like its mammalian counterparts, bcRIPK3 exhibited a conserved function in inducing cell death. The reporter assay and plaque assay showed that overexpression of bcRIPK3 restricted bcMAVS-activated transcription of the interferon promoters of black carp and zebrafish, and suppressed bcMAVS-mediated antiviral activity. Notably, EPC cells co-expressing bcRIPK3, bcRIPK1 and bcMAVS presented much attenuated antiviral activity than the cells co-expressing bcRIPK3 and bcMAVS; and the subsequent co-IP assay identified the interaction between bcRIPK3 and bcRIPK1. Our findings collectively elucidate for the first time in teleost that black carp RIPK3 interacts with RIPK1 to inhibit MAVS-mediated antiviral signaling.
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http://dx.doi.org/10.1016/j.fsi.2021.06.011DOI Listing
August 2021

Declining utilization of potentially inappropriate medications in older adults by dermatologists: A cross-sectional Medicare analysis.

J Am Acad Dermatol 2021 Jun 16. Epub 2021 Jun 16.

Department of Dermatology, University of Connecticut Health Center, Farmington, Connecticut. Electronic address:

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http://dx.doi.org/10.1016/j.jaad.2021.06.839DOI Listing
June 2021

Hot-Casting Large-Grain Perovskite Film for Efficient Solar Cells: Film Formation and Device Performance.

Nanomicro Lett 2020 Jul 31;12(1):156. Epub 2020 Jul 31.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu, 610054, People's Republic of China.

Organic-inorganic metal halide perovskite solar cells (PSCs) have recently been considered as one of the most competitive contenders to commercial silicon solar cells in the photovoltaic field. The deposition process of a perovskite film is one of the most critical factors affecting the quality of the film formation and the photovoltaic performance. A hot-casting technique has been widely implemented to deposit high-quality perovskite films with large grain size, uniform thickness, and preferred crystalline orientation. In this review, we first review the classical nucleation and crystal growth theory and discuss those factors affecting the hot-casted perovskite film formation. Meanwhile, the effects of the deposition parameters such as temperature, thermal annealing, precursor chemistry, and atmosphere on the preparation of high-quality perovskite films and high-efficiency PSC devices are comprehensively discussed. The excellent stability of hot-casted perovskite films and integration with scalable deposition technology are conducive to the commercialization of PSCs. Finally, some open questions and future perspectives on the maturity of this technology toward the upscaling deposition of perovskite film for related optoelectronic devices are presented.
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http://dx.doi.org/10.1007/s40820-020-00494-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770834PMC
July 2020

Suppressive role of microRNA-130b-3p in ferroptosis in melanoma cells correlates with DKK1 inhibition and Nrf2-HO-1 pathway activation.

Hum Cell 2021 Jun 11. Epub 2021 Jun 11.

Department of Ultrasound, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, Changsha, 410005, Hunan Province, People's Republic of China.

Cell death pathways related to ferroptosis are implicated in the progression of melanoma. Emerging data reporting the upregulation of microRNA (miR)-130b-3p in melanoma indicate the potential implication of miR-130b-3p in this malignancy. Herein, we aimed to identify whether and how miR-130b-3p regulated ferroptosis in melanoma cells. Melanoma cells (A375, G-361) were treated with erastin or RSL3 to mimic ferroptosis in vitro. Viability, lipid peroxidation level and ferrous ion content in melanoma cells were then assessed in response to manipulation of miR-130b-3p expression. Luciferase assay was conducted to determine the binding of miR-130b-3p to Dickkopf1 (DKK1). Western blot assay was conducted to determine the expression of molecules related to nuclear factor-erythroid 2 p45-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. The results indicated that miR-130b-3p exerted an inhibitory role in erastin or RSL3-induced ferroptosis, evidenced by reductions in lipid peroxidation and ferrous ion content. By suppressing the expression of target gene DKK1, miR-130b-3p activated the Nrf2/HO-1 pathway, whereby repressing ferroptosis. miR-130b-3p blocked the antitumor activity of erastin. Further, in vitro findings were reproduced in an in vivo murine model. Together, these data suggest the potential of miR-130b-3p to inhibit ferroptosis in melanoma cells and the mechanism was related to DKK1-mediated Nrf2/HO-1 pathway.
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http://dx.doi.org/10.1007/s13577-021-00557-5DOI Listing
June 2021

Somatic Mutation Profiling of Papillary Thyroid Carcinomas by Whole-exome Sequencing and Its Relationship with Clinical Characteristics.

Int J Med Sci 2021 26;18(12):2532-2544. Epub 2021 Apr 26.

Department of Neurosurgery, the Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225012, China.

The incidence of papillary thyroid carcinomas (PTCs) has increased rapidly during the past several decades. Until now, the mechanisms underlying the tumorigenesis of PTCs have remained largely unknown. Next-generation-sequencing (NGS) provides new ways to investigate the molecular pathogenesis of PTCs. To characterize the somatic alterations associated with PTCs, we performed whole-exome sequencing (WES) of PTCs from 23 Chinese patients. This study revealed somatic mutations in genes with relevant functions for tumorigenesis, such as BRAF, BCR, CREB3L2, DNMT1, IRS2, MSH6, and TP53. We also identified novel somatic gene alterations which may be potentially involved in PTC progression. Gene set enrichment analysis revealed that the cellular response to hormone stimulus, epigenetic modifications, such as protein/histone methylation and protein alkylation, as well as MAPK, PI3K-AKT, and FoxO/mTOR signaling pathways, were significantly altered in the PTCs studied here. Moreover, Protein-Protein Interaction (PPI) network analysis of our mutated gene selection highlighted EP300, KRAS, PTEN, and TP53 as major core genes. The correlation between gene mutations and clinicopathologic features of the PTCs defined by conventional ultrasonography (US) and contrast-enhanced ultrasonography (CEUS) were assessed. These analyses established significant associations between subgroups of mutations and respectively taller-than-wide, calcified, and peak time iso- or hypo-enhanced and metastatic PTCs. In conclusion, our study supplements the genomic landscape of PTCs and identifies new actionable target candidates and clinicopathology-associated mutations. Extension of this study to larger cohorts will help define comprehensive genomic aberrations in PTCs and validate target candidates. These new targets may open methods of individualized treatments adapted to the clinicopathologic specifics of the patients.
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http://dx.doi.org/10.7150/ijms.50916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176168PMC
April 2021

Stable isotopes of deep soil water retain long-term evaporation loss on China's Loess Plateau.

Sci Total Environ 2021 Aug 17;784:147153. Epub 2021 Apr 17.

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest A&F University, Yangling, Shaanxi Province 712100, China.

Evaporation from the land surface enriches heavy isotope ratios (H/H and O/ O) in shallow soils, and downward water movement will carry the fractionation signal to deep soils. However, how to acquire the evaporation from water stable isotopes in deep soils remains untested. Here, we measured water stable isotope composition in the deep soils (2-10 m) across 20 sites on China's Loess Plateau. Our results show that the line-conditioned excess (lc-excess) in deep soils of these sites was invariable with depth at each site, but ranged between -14.0‰ and - 4.1‰ among these sites, indicating differing degree of enrichment in heavy water isotopes between sites. Moreover, the mean lc-excess in deep soils water was significantly correlated to mean annual precipitation (R = 0.57), potential evapotranspiration (R = 0.25), and the Budyko dryness (R = 0.68), indicating that deep soil water lc-excess reflects land surface climate conditions. Furthermore, the deep soils correspond to a timescale of approximately 100 years at one site and more than 27 years at the remaining sites. These results together indicate that stable isotopes of deep soil water retained long-term land surface evaporation effects. Further, by implementing the steady-state isotope mass balance model into the lc-excess framework, we derived a new method to estimate evaporation loss fraction (f). Our f estimates at these sites varied between 5% and 15%, which may represent the lower bound of the actual evaporation to precipitation ratio. Nevertheless, our work suggests that in these and the other similar regions, deep soil is a novel archive for long-term soil evaporation loss, and f may be estimated through a snapshot field campaign of stable isotope measurements.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147153DOI Listing
August 2021

Trends in utilization of reflectance confocal microscopy in the United States, 2017-2019.

J Am Acad Dermatol 2021 Jun 1. Epub 2021 Jun 1.

Department of Dermatology, University of Connecticut Health Center, Farmington, Connecticut. Electronic address:

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http://dx.doi.org/10.1016/j.jaad.2021.04.104DOI Listing
June 2021

The Efficacy and Safety of Fractional 1064 nm Nd:YAG Picosecond Laser Combined With Intense Pulsed Light in the Treatment of Atrophic Acne Scar: A Split-Face Study.

Lasers Surg Med 2021 Jun 3. Epub 2021 Jun 3.

Department of Dermatology, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Wulumuqi Road, Shanghai, 200040, China.

Background And Objectives: To evaluate the efficacy and safety of fractional 1064 nm Nd:YAG picosecond laser combined with intense pulsed light (IPL) in the treatment of atrophic acne scar with post-inflammatory erythema (PIE). © 2021 Wiley Periodicals LLC.

Study Design/materials And Methods: Seventeen patients received five sessions of treatment at weeks 0, 4, 8, 12, 16 and were followed up at week 28. One half of the face was randomly treated by fractional 1064 nm Nd:YAG picosecond laser combined with IPL (FxPico + IPL), and the other by IPL alone as a control.

Results: For the 15 patients who completed the study, the FxPico + IPL side demonstrated significant median Échelle D'évaluation clinique des cicatrices D'acné (ECCA) score improvement (P < 0.01), while IPL alone side did not (P = 0.1250). The pore counts for both sides decreased but more pore count reduction was seen on the FxPico + IPL side (P < 0.05). Better scar improvement was observed on the FxPico + IPL-treated side (P < 0.05) while no difference in erythema improvement was seen between the two sides. There was no difference between the two treatments in terms of overall satisfaction. Pain, erythema, edema, petechiae, crusting, reactive acneiform eruptions, and pruritus were common adverse effects and were mild to moderate.

Conclusion: FxPico + IPL is an effective, efficient, and safe treatment regimen for atrophic acne scars complicated by PIE.
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http://dx.doi.org/10.1002/lsm.23428DOI Listing
June 2021

Outcomes of Eyes Lost to Follow-Up in Patients with Central Retinal Vein Occlusion Who are Receiving Anti-Vascular Endothelial Growth Factor Treatment.

Ther Clin Risk Manag 2021 26;17:489-496. Epub 2021 May 26.

Department of Ophthalmology, The First Hospital of China Medical University, Shenyang, People's Republic of China.

Background: To evaluate the anatomic and functional outcomes in eyes of patients with macular edema (ME) caused by central retinal vein occlusion (CRVO) who were lost to follow-up (LTFU) for more than 6 months following treatment with anti-vascular endothelial growth factor (VEGF) therapy and to determine the predictive factors of visual prognosis in these patients.

Methods: This study was conducted as a retrospective, case series investigation. Patients whose eyes were receiving intravitreal anti-VEGF treatment for CRVO-ME, with the next follow-up visit occurring more than 6 months following treatment were identified. Baseline disease characteristics (at the last visit before being LTFU), cause and duration of treatment interruption, and the resulting disease progression, complications, and outcomes were assessed. Baseline characteristics predictive of visual outcome were also analyzed.

Results: This study included a total of 17 eyes of 17 patients. The mean duration of being LTFU was 7.8 ± 2.1 months. On the return visit after being LTFU, 7 of 17 eyes (41.2%) developed neovascular complications. Despite treatment, 12 eyes (70.1%) lost ≥3 best-corrected visual acuity (BCVA) lines, with 2 eyes (11.8%) developing a final BCVA of hand motion or more severe. At the final visit, the mean logarithm of minimal angle of resolution (logMAR) BCVA deteriorated significantly compared to before being LTFU (P < 0.001). The increasing duration of being LTFU is associated with a deterioration of visual acuity prognosis.

Conclusion: In CRVO-ME patients who are receiving anti-VEGF therapy, unintentional treatment interruptions can cause visually disastrous consequences, including irreversible blindness. Patients who were LTFU for a long period should be strongly warned about their poor visual prognosis.
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http://dx.doi.org/10.2147/TCRM.S303248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165207PMC
May 2021

Fluorinated Oligomer Wrapped Perovskite Crystals for Inverted MAPbI Solar Cells with 21% Efficiency and Enhanced Stability.

ACS Appl Mater Interfaces 2021 Jun 30;13(22):26093-26101. Epub 2021 May 30.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, China.

Defects at the grain boundary provide sites for nonradiative recombination in halide perovskite solar cells (PSCs). Here, by polymerization and fluorination of a Lewis acid of 4,4-bis(4-hydroxyphenyl)pentanoic acid, a fluorinated oligomer (FO-19) is synthesized and applied to passivate these defects in methlyammonium lead iodide (MAPbI). It is demonstrated that the carboxyl bond of FO-19 was coordinated with Pb ions in the perovskite films to achieve a wrapping effect on the perovskite crystals. The defects of perovskite film are effectively passivated, and the undesirable nonradiative recombination is greatly inhibited. As a result, FO-19 gives a power conversion efficiency of 21.23% for the inverted MAPbI-based PSCs, which is among the highest reported values in the literature. Meanwhile, the corresponding device with FO-19 exhibits significantly improved humidity and thermal stability. Therefore, this work offers insights into the realization of high-efficiency and stable PSCs through fluorinated additive engineering.
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http://dx.doi.org/10.1021/acsami.1c06216DOI Listing
June 2021

Reply to: Metformin use and keratinocyte carcinoma risk.

J Am Acad Dermatol 2021 May 27. Epub 2021 May 27.

Faculty of Medicine, University of Iceland, Reykjavik, Iceland; Department of Pathology, Landspitali National-University Hospital, Reykjavik, Iceland.

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http://dx.doi.org/10.1016/j.jaad.2021.05.028DOI Listing
May 2021

Diversity and antibacterial activity of fungal endophytes from Eucalyptus exserta.

BMC Microbiol 2021 May 27;21(1):155. Epub 2021 May 27.

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 510642, No. 483, Wushan Road, Tianhe District, Guangdong, 510642, Guangzhou, China.

Background: Eucalyptus bacterial wilt caused by Ralstonia solanacearum is an important eucalyptus disease. Endophytic fungi, an important source of natural active substances, provide a new breakthrough for the control of plant diseases.

Results: In the present study, 80 endophytic fungal isolates were obtained from the healthy branches and fruits of Eucalyptus exserta. Fifteen distinct isolates (MK120854-MK120868) were selected for further taxonomic identification through morphological trait assessments and internal transcribed spacer (ITS) region-rRNA gene sequence analysis. Thirteen genera, namely, Phyllosticta, Penicillium, Eutypella, Purpureocillium, Talaromyces, Lophiostoma, Cladosporium, Pestalotiopsis, Chaetomium, Fusarium, Gongronella, Scedosporium and Pseudallescheria, were identified on the basis of their morphological characteristics. Members of the genus Phyllosticta were the primary isolates, with a colonization frequency (CF) of 27.5 %. Most of the fungal isolates displayed antibacterial activity. The crude extracts obtained from Lophiostoma sp. Eef-7, Pestalotiopsis sp. Eef-9 and Chaetomium sp. Eef-10 exhibited strong inhibition on the test bacteria, and Lophiostoma sp. Eef-7 was further cultured on a large scale. Three known compounds, scorpinone (1), 5-deoxybostrycoidin (2) and 4-methyl-5,6-dihydro-2 H-pyran-2-one (3), were isolated from the endophytic fungus Lophiostoma sp. Eef-7 associated with E. exserta. The structures of these compounds were elucidated by analysis of 1D and 2D NMR and HR-ESI-MS spectra and a comparison of their spectral data with published values. Compounds 1 and 2 showed weak antimicrobial activity against Ralstonia solanacearum.

Conclusions: Endophytic fungi from Eucalyptus exserta may represent alternative sources of antimicrobial agents. Lophiostoma sp. Eef-7 can produce 2-azaanthraquinone derivatives and shows weak antibacterial activity against Ralstonia solanacearum.
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http://dx.doi.org/10.1186/s12866-021-02229-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157698PMC
May 2021

Heating events drive the seasonal patterns of volatile organic compounds in a typical semi-arid city.

Sci Total Environ 2021 Sep 19;788:147781. Epub 2021 May 19.

School of Ecology and Environment, Inner Mongolia University, 010021 Hohhot, China; Institute of Environmental Geology, Inner Mongolia University, Hohhot 010021, China. Electronic address:

The emission characteristics, source apportionment and chemical behavior of volatile organic compounds (VOCs) are important for strategy-making on ozone (O) and fine particulate matter (PM) control. Based on the continuous observation during four seasons, the seasonal characteristics, chemical reactivity and source apportionment of 116 VOCs species were studied in a typical semi-arid city with no relevant research. The results showed that the annual average concentrations of total volatile organic compounds (TVOCs) in Hohhot was 44.67 ± 46.59 ppbv with the predominant of alkanes and oxygenated volatile organic compounds (OVOCs). The sharp increment of TVOCs were explained by the elevating OVOCs and alkanes in autumn, while alkanes and alkenes in winter. The levels of alkenes presented negative and positive correlations with solar radiation and PM, respectively. The mixing ratios accounted for 30% (alkanes) and 23% (alkenes and aromatics) of the TVOCs, respectively; while their ozone formation potential (OFP) ~15% and nearly 50% (even 75% in winter), respectively, indicating that the OFP of different VOCs species depends not only on their concentrations but more importantly on their chemical activity in atmosphere. According to the seasonal source apportionment, both the high levels of short-chain alkanes, alkenes and aromatics and the increasing coal sales volume suggested that the combustion sources were the predominant in heating seasons, while solvent uses was extracted as the most predominant during non-heating seasons. In non-heating seasons, the biogenic emission sources, ranking as the second contributor, were significantly higher than heating seasons. Isoprene was the most active biogenic VOCs species, bagging test results showed that deciduous trees were the predominant contributors for isoprene (~99%), while coniferous trees and shrub for monoterpenes (>95%). It will be helpful for understanding the characteristics of VOCs in Chinese national key development areas and informing policy to control semi-arid regional VOCs air pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147781DOI Listing
September 2021

Positivity and Stability Analysis of T-S Fuzzy Descriptor Systems With Bounded and Unbounded Time-Varying Delays.

IEEE Trans Cybern 2021 May 24;PP. Epub 2021 May 24.

This work focuses on the stability analysis of positive Takagi-Sugeno (T-S) fuzzy descriptor systems with time-varying delays. An equivalent augmented system is constructed to investigate the positivity and stability of T-S fuzzy descriptor time-delay systems. By using this transformed system, a necessary and sufficient positivity condition is first derived for systems, which can be verified by linear programming (LP). Then, based on the positivity of T-S fuzzy descriptor systems, a sufficient condition is put forward for the asymptotic stability of systems with bounded and unbounded time-varying delays. Finally, several examples are provided to show the effectiveness of the obtained results.
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http://dx.doi.org/10.1109/TCYB.2021.3072392DOI Listing
May 2021

Black carp TUFM collaborates with NLRX1 to inhibit MAVS-mediated antiviral signaling pathway.

Dev Comp Immunol 2021 Sep 14;122:104134. Epub 2021 May 14.

State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China. Electronic address:

TUFM is a mitochondrial protein and serves as a regulator of antiviral signaling; nevertheless, the character of TUFM in teleosts remains unidentified. In this study, TUFM homologue of black carp (Mylopharyngodon piceus) has been characterized and its role in innate immunity has been explored. Black carp TUFM (bcTUFM) comprises 447 amino acids and shows the high similarity to human TUFM. bcTUFM was about 50 kDa in the Western blot assay and was determined as a cytosolic protein by immunofluorescent microscopy. Knockdown of bcTUFM by shRNA enhanced the antiviral ability of the host cells. The induction fold of interferon promoter transcription in the cells co-expressing bcTUFM and bcMAVS was much lower than that of the cells expressing bcMAVS alone. Our previous study has identified that bcNLRX1 interacted with bcMAVS and functioned as an inhibitor of bcMAVS. The interaction between bcTUFM and bcNLRX1, but not bcTUFM and bcMAVS, was detected through co-immunoprecipitation. The subsequent reporter assay and plaque assay demonstrated that the inhibition of bcMAVS-mediated interferon production and antiviral activity by bcNLRX1 was enhanced by co-expressed bcTUFM. Thus, our data suggests that bcTUFM cooperates with bcNLRX1 to inhibit bcMAVS-mediated antiviral signaling during host antiviral innate immune response against SVCV.
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http://dx.doi.org/10.1016/j.dci.2021.104134DOI Listing
September 2021

Annual Trends in Medicare Part D Prescription Claims for Vismodegib, 2013-2018.

J Am Acad Dermatol 2021 May 14. Epub 2021 May 14.

Department of Dermatology, University of Connecticut, Farmington, Connecticut. Electronic address:

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http://dx.doi.org/10.1016/j.jaad.2021.05.012DOI Listing
May 2021

Identifying the Potential Therapeutic Targets for Atopic Dermatitis Through the Immune Infiltration Analysis and Construction of a ceRNA Network.

Clin Cosmet Investig Dermatol 2021 7;14:437-453. Epub 2021 May 7.

Department of Dermatology, The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital, Changsha, People's Republic of China.

Purpose: This study was meant to analyze immune infiltration and construct a ceRNA network to explore the new therapeutic targets for atopic dermatitis (AD) through bioinformatics way.

Patients And Methods: We downloaded the AD patients' RNA expression profile datasets (GSE63741, GSE124700) from the Gene Expression Omnibus (GEO) database, which were analyzed through the GEO2R. We explored the hub genes by the enrichment analysis and the protein-protein interaction (PPI) analysis. Moreover, we estimated immune cell types and their proportions by ImmucellAI. GSE121212 dataset validation was performed to verify the robustness of the hub genes. Then, a ceRNA network was constructed by the miRWalk, miRNet, miRDB, DIANA, TargetScan, and starbase database. Finally, gene expression analysis was performed by using RT-qPCR.

Results: In total, we detected 22 differentially expressed genes (DEGs), which contained 8 downregulated genes and 14 upregulated genes. There were 5 hub genes confirmed as key genes through PPI network analysis and the ROC curves. KEGG pathway analysis revealed that they were significantly enriched in the IL-17 signaling pathway and GO analysis showed mainly in the immune cell chemotaxis. The immune infiltration profiles were different between normal controls and AD, and each of the key genes (S100A7, S100A8, S100A9, and LCE3D) was significantly correlated with the main infiltration cell of AD. A lncRNA-miRNA-mRNA ceRNA network containing the key genes was constructed, and NEAT1 and XIST, the core of ceRNA network, were significantly overexpressing verified by RT-qPCR in AD patients.

Conclusion: Altogether, the key genes and their ceRNA network provided a novel perspective to the immunomodulation of AD, which may be potential and new therapeutic targets for AD.
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http://dx.doi.org/10.2147/CCID.S310426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112859PMC
May 2021

Functional characterization of an interleukin 20 like homologue in grass carp Ctenopharyngodon idella.

Fish Shellfish Immunol 2021 Aug 13;115:43-57. Epub 2021 May 13.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

IL-20 is a pleiotropic cytokine that belongs to the IL-10 family and plays an important biological role in tissue homeostasis and regulation of host immune defenses. IL-20 homologues have recently been discovered in fish, but their functions have not been studied. In this study, an IL-20 like (IL-20L) cytokine was cloned in grass carp (Ctenopharyngodon idella) and its bioactivities were investigated. Expression analysis showed that the CiIL-20L gene was constitutively expressed in tissues with the highest expression detected in the head kidney. It was upregulated in the head kidney after infection with Flavobactrium columnare (F. cloumnare) and grass carp reovirus II (GCRV II). The recombinant CiIL-20L produced in E. coli cells was shown to be effective in inducing the expression of Th cytokine genes (IFN-γ, IL-4/13A, IL-4/13B and IL-10), macrophage marker genes (arginase 2, IRF4, KLF4 and SOCS3) and inflammatory genes (IL-1β, IL-6, IL-8 and TNFα) in the head kidney leukocytes when stimulated at 12 h. Long term culture (6 days) of head kidney macrophages in the presence of CiIL-20L leads to high expression of IRF4, TGFβ1 and arginase 2. Our data suggest that IL-20 may play regulatory roles in promoting Th responses, macrophage differentiation and inflammation.
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http://dx.doi.org/10.1016/j.fsi.2021.05.009DOI Listing
August 2021
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