Publications by authors named "Feng Han"

589 Publications

Agent-based model for simulation of the sustainability revolution in eco-industrial parks.

Environ Sci Pollut Res Int 2021 Nov 20. Epub 2021 Nov 20.

Institute of Science and Technology for Development of Shandong, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250100, China.

Eco-industrial parks (EIPs) are of increasing importance for implementing industrial ecology strategies and are facing increasing challenges in terms of environmental pollution and resource scarcity. As a complex adaptive system, an EIP involves multiple sectors and faces various disturbances that influence its evolutionary trajectories. This study adopts an agent-based model to simulate the material flows and industrial symbiosis process in the EIP, considering the initiative of each company and the ever-changing environment. The proposed EIP model emphasises the heterogeneity of companies and attempts to reflect multiple and dynamic factors that have received less attention in previous studies. This model contains two types of agents, companies and the external environment. A company agent makes decisions and interacts with other agents following its own behaviour rules, while the external environment agent functions to coordinate the material flows and exert influence on the companies. The model has been verified and validated by simulating a 20-year-period development of an empirical EIP in China. The simulation results are assessed by three indicators: eco-connectance, eco-efficiency, and industrial symbiosis indicator. Results showed that during the growing phase, the eco-connectance increased from 0.02 to 0.1 for the non-disturbance situation. The eco-efficiency and industrial symbiosis indicator also realised 78.5% and 74.8% of their total increments. The outcome of this research provides insights for the design of the strategies to improve the industrial symbiosis performance and is of high potential to facilitate EIPs in promoting eco-transformation and sustainable development.
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http://dx.doi.org/10.1007/s11356-021-17503-5DOI Listing
November 2021

[A novel alkaloid from Corydalis tomentella].

Zhongguo Zhong Yao Za Zhi 2021 Oct;46(19):5020-5026

College of Pharmaceutical Sciences, Southwest University Chongqing 400715, China.

The chemical constituents in the ethyl acetate extract of Corydalis tomentella was isolated and purified with normal and reversed phase silica gel column chromatography, Sephadex LH-20, MCI, and semi-preparative HPLC. The compound structures were identified based on spectroscopic experiments and reported papers. Finally, eighteen compounds(1-18) were obtained from C. tomentella, including 17 alkaloids and 1 terpenoid. Among them, compound 1(tomentellaine A) was a novel alkaloid. Compounds 2-5, 7-14, and 16-18 were isolated from this plant for the first time.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210706.201DOI Listing
October 2021

A Terahertz Detector Based on Double-Channel GaN/AlGaN High Electronic Mobility Transistor.

Materials (Basel) 2021 Oct 18;14(20). Epub 2021 Oct 18.

State Key Laboratory of Mechanical Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

A double-channel (DC) GaN/AlGaN high-electron-mobility transistor (HEMT) as a terahertz (THz) detector at 315 GHz frequency is proposed and fabricated in this paper. The structure of the epitaxial layer material in the detector is optimized, and the performance of the GaN HEMT THz detector is improved. The maximum responsivity of 10 kV/W and minimum noise equivalent power (NEP) of 15.5 pW/Hz are obtained at the radiation frequency of 315 GHz. The results are comparable to and even more promising than the reported single-channel (SC) GaN HEMT detectors. The enhancement of THz response and the reduction of NEP of the DC GaN HEMT detector mainly results from the interaction of 2DEG in the upper and lower channels, which improves the self-mixing effect of the detector. The promising experimental results mean that the proposed DC GaN/AlGaN HEMT THz detector is capable of the practical applications of THz detection.
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http://dx.doi.org/10.3390/ma14206193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539176PMC
October 2021

Cross-Sectional Analysis of the Involvement of Interleukin-17A in Diabetic Retinopathy in Elderly Individuals with Type 2 Diabetes Mellitus.

Diabetes Metab Syndr Obes 2021 9;14:4199-4207. Epub 2021 Oct 9.

Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People's Republic of China.

Background: To investigate the correlation between serum interleukin-17A (IL-17A) levels and diabetic retinopathy (DR) in elderly individuals with type 2 diabetes mellitus (T2DM).

Methods: The study included 194 elderly patients (94 males and 100 females) with T2DM. Digital retinal photography as well as fundus fluorescein angiography was employed to distinguish between nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR). In addition, multiple logistic regression analysis was conducted to determine the correlation between serum IL-17A levels and DR status.

Results: The average age of the study cohort was 69.14 ± 6.33 years, of which 52.08% were male. The study participants with the highest IL-17A (Q4) levels had higher TC, DBP, and low-density lipoprotein cholesterol (LDL-C) values than those the other groups. Analysis using unadjusted and adjusted linear regression revealed that the effect size of 1.09 for DR in the unadjusted model indicates that IL-17A is associated with an increase of 1.09 in DR (mmol/L) (β 1.09, 95% confidence interval (CI) 1.03, 1.16). Using the minimum-adjusted model (the model 2), as IL-17A increased, DR was higher by 1.11 (β 1.11, 95% CI 1.04, 1.18). With the fully adjusted model (the model 3), for each additional IL-17A increase, DR was higher by 1.15 (β 1.15, 95% CI 1.06, 1.24).

Conclusion: Serum IL-17A levels are apparently positively correlated to DR in Chinese elderly individuals with T2DM.
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http://dx.doi.org/10.2147/DMSO.S302199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517528PMC
October 2021

Enhancing the Mechanical Strength of Electrolyte-Supported Solid Oxide Cells with Thin and Dense Doped-Ceria Interlayers.

ACS Appl Mater Interfaces 2021 Oct 13;13(42):49879-49889. Epub 2021 Oct 13.

German Aerospace Centre (DLR), Institute of Engineering Thermodynamics, Pfaffenwaldring 38-40, 70569 Stuttgart, Germany.

The penetration of fuel cells and electrolyzers in energy systems calls for their scale-up to the gigawatt (GW) level. High temperature solid oxide cells (SOC) offer unrivaled efficiencies in both electrolysis and fuel cell operation. However, they are made of ceramics and are brittle by nature. Consequently, a high mechanical strength to avoid failure during stacking is essential to achieve a high manufacturing yield. Here, we show that without changing the materials of the cells, thin and dense ceria interlayers enable comparable power densities and durability in fuel cell operation. The sole tuning of the morphology and processing of the interlayers reduce the residual stress in the cell significantly which increases its mechanical strength by up to 78%. These results promise performance gains of similar magnitude by enabling a substantial decrease of the electrolyte thickness while maintaining robustness. This stress engineering approach presents a way to increase the volumetric power density and material efficiency of SOC systems.
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http://dx.doi.org/10.1021/acsami.1c13899DOI Listing
October 2021

Cell-derived extracellular matrix materials for tissue engineering.

Tissue Eng Part B Rev 2021 Oct 12. Epub 2021 Oct 12.

Chinese PLA General Hospital, 104607, Institute of Orthopedics; Beijing Key Lab of Regenerative Medicine in Orthopedics; Key Lab of Musculoskeletal Trauma & War Injuries, Beijing, China.

The involvement of cell-derived extracellular matrix (CDM) in assembling tissue engineering scaffolds has yielded significant results. CDM possesses excellent characteristics, such as ideal cellular microenvironment mimicry and good biocompatibility, which make it a popular research direction in the field of bionanomaterials. CDM has significant advantages as an expansion culture substrate for stem cells, including stabilization of phenotype, reversal of senescence, and guidance of specific differentiation. In addition, the applications of CDM-assembled tissue engineering scaffolds for disease simulation and tissue organ repair are comprehensively summarized; the focus is mainly on bone and cartilage repair, skin defect or wound healing, engineered blood vessels, peripheral nerves, and periodontal tissue repair. We consider CDM a highly promising bionic biomaterial for tissue engineering applications and propose a vision for its comprehensive development.
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http://dx.doi.org/10.1089/ten.TEB.2021.0147DOI Listing
October 2021

Endothelial peroxynitrite causes disturbance of neuronal oscillations by targeting caspase-1 in the arcuate nucleus.

Redox Biol 2021 Nov 27;47:102147. Epub 2021 Sep 27.

Key Laboratory of Cardiovascular & Cerebrovascular Medicine, Drug Target and Drug Discovery Center, School of Pharmacy, Nanjing Medical University, Nanjing 211166, China; Gusu School, Nanjing Medical University, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, China; Institute of Brain Science, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing 211166, China. Electronic address:

Severe anorexia limits the clinical application of cisplatin, and even leads to the discontinuation of treatment. However, the mechanisms underlying cisplatin-induced anorexia are unknown. Herein, we demonstrated that cisplatin could affect neuronal gamma oscillations and induce abnormal neuronal theta-gamma phase-amplitude coupling in the arcuate nucleus (Arc) of the hypothalamus, and these findings were associated with significantly decreased food intake and weight loss in mice. Chemogenetic activation of AgRP neurons in the Arc reversed the cisplatin-induced food intake reduction in mice. We further demonstrated that endothelial peroxynitrite (ONOO) formation in the Arc induced nitrosative stress following cisplatin treatment via a previously uncharacterized pathway involving neuronal caspase-1 activation. Strikingly, treatment with the ONOO scavenger uric acid (UA) reversed the reduced action potential (AP) frequency of AgRP neurons and increased the AP frequency of POMC neurons induced by SIN1, a donor of ONOO, in the Arc, as determined by whole-cell patch-clamp electrophysiological recording. Consistent with these findings, UA treatment effectively alleviated cisplatin-induced dysfunction of neuronal oscillations and neuronal theta-gamma phase-amplitude coupling in the Arc of mice. Taken together, these results suggest, for the first time, that targeting the overproduction of endothelial ONOO can regulate cisplatin-induced neurotoxicity through neuronal caspase-1, and thereby serve as a potential therapeutic approach to alleviate chemotherapy-induced anorexia and weight loss.
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http://dx.doi.org/10.1016/j.redox.2021.102147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495174PMC
November 2021

BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice.

Sci Rep 2021 Sep 29;11(1):19375. Epub 2021 Sep 29.

Department of Human Histology and Embryology, School of Basic Medical Sciences, Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University (North Campus), Guiyang, 550025, Guizhou, China.

Rheumatoid arthritis (RA) is an autoimmune disorder characterized by persistent inflammatory responses in target tissues and organs, resulting in the destruction of joints. Collagen type II (CII)-induced arthritis (CIA) is the most used animal model for human RA. Although BTN2A2 protein has been previously shown to inhibit T cell functions in vitro, its effect on autoimmune arthritis has not been reported. In this study, we investigate the ability of a recombinant BTN2A2-IgG2a Fc (BTN2A2-Ig) fusion protein to treat CIA. We show here that administration of BTN2A2-Ig attenuates established CIA, as compared with control Ig protein treatment. This is associated with reduced activation, proliferation and Th1/Th17 cytokine production of T cells in BTN2A2-Ig-treated CIA mice. BTN2A2-Ig also inhibits CII-specific T cell proliferation and Th1/Th17 cytokine production. Although the percentage of effector T cells is decreased in BTN2A2-Ig-treated CIA mice, the proportions of naive T cells and regulatory T cells is increased. Furthermore, BTN2A2-Ig reduces the percentage of proinflammatory M1 macrophages but increases the percentage of anti-inflammatory M2 macrophages in the CIA mice. Our results suggest that BTN2A2-Ig protein has the potential to be used in the treatment of collagen-induced arthritis models.
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http://dx.doi.org/10.1038/s41598-021-98443-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481265PMC
September 2021

A System Dynamics Model for Urban Residential Building Stock towards Sustainability: The Case of Jinan, China.

Int J Environ Res Public Health 2021 09 9;18(18). Epub 2021 Sep 9.

Energy Research Institute of Shandong Academy of Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250100, China.

Resource and environmental issues related to urban building systems have recently become a hot research topic in the field of urban environmental management research. Taking Jinan city as an example, this paper establishes a system dynamic model for an urban residential building stock system. The simulated results show that the urban residential building stock will be 1.99 × 10 m in 2050; and the annual total demolition buildings will be at 3.36 × 10 m in 2082. Policy measures were developed based on four important action fields such as per capita floor area (PCFA), the building structure proportion of new construction, lifetime of the residential building, and the recycling of the C&D waste. Among these approaches, the set of policy measures focusing on the recycling of C&D waste appears to be more effective in reducing environmental and resource impacts than the other three fields. It is also found that the recycling of brick and concrete waste plays a considerable role in reducing environment and resource impacts due to the development of urban residential building stock with the lapse of time.
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http://dx.doi.org/10.3390/ijerph18189520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472286PMC
September 2021

Olanzapine: Association Between a Typical Antipsychotic Drug and Aortic Calcification.

Front Cardiovasc Med 2021 10;8:710090. Epub 2021 Sep 10.

Department of Radiation Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

This study concentrates on the relationship between antipsychotic drugs (APDs) and aortic calcification. All 56 patients with schizophrenia were divided into two groups according to aortic calcification index. APD equivalent dose was calculated via defined daily doses method. In schizophrenia patients with higher aortic calcification index scores, APD equivalent doses were lower. APD equivalent dose was negatively related to aortic calcification index. Although equivalent APD dose in patients without olanzapine treatment was negatively related to aortic calcification index, it seems that equivalent APD dose did not associate with aortic calcification. Aortic calcification is negatively associated with APD dose in schizophrenia patients. Olanzapine seems to be vital to the relationship between aortic calcification and APD treatment.
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http://dx.doi.org/10.3389/fcvm.2021.710090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460775PMC
September 2021

Pulmonary Benefits of Intervention with Air Cleaner among Schoolchildren in Beijing: A Randomized Double-Blind Crossover Study.

Environ Sci Technol 2021 Sep 7. Epub 2021 Sep 7.

Key Laboratory of Environment and Human Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.

We conducted a crossover study employing air cleaner intervention among 125 schoolchildren aged 9-12 years in a boarding school in Beijing, China. The PM concentrations were monitored, and 27 biomarkers were analyzed. We used the linear mixed-effects model to evaluate the association of intervention/time-weighted PM concentrations with biomarkers. The outcomes showed that air cleaner intervention was associated with FeNO, exhaled breath condensate (EBC) IL-1β, and IL-6, which decreased by 12.57%, 10.83%, and 4.33%, respectively. Similar results were observed in the associations with PMs. Lag 1 day PMs had the strongest relationship with biomarkers, and significant changes were observed in biomarkers such as FEV, FeNO, EBC 8-iso, and MCP-1. Boys showed higher percentage changes than girls, and the related biomarkers were FeNO, EBC 4-HNE, IL-1β, IL-6, and MCP-1. The results showed that biomarkers such as FeNO, EBC IL-6, MCP-1, and 4-HNE could sensitively reflect the early abnormal response of the respiratory system under short-term PM exposure among healthy schoolchildren and indicated that (1) air cleaners exert a protective effect on children's respiratory system. (2) PM had lag and cumulative effect, lag 1 day had the greatest effect. (3) The boys were more sensitive than the girls.
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http://dx.doi.org/10.1021/acs.est.1c03146DOI Listing
September 2021

Structures of heat shock factor trimers bound to DNA.

iScience 2021 Sep 5;24(9):102951. Epub 2021 Aug 5.

Institute of Immunology, Army Medical University, Chongqing 400038, China.

Heat shock factor 1 (HSF1) and 2 (HSF2) play distinct but overlapping regulatory roles in maintaining cellular proteostasis or mediating cell differentiation and development. Upon activation, both HSFs trimerize and bind to heat shock elements (HSEs) present in the promoter region of target genes. Despite structural insights gained from recent studies, structures reflecting the physiological architecture of this transcriptional machinery remains to be determined. Here, we present co-crystal structures of human HSF1 and HSF2 trimers bound to DNA, which reveal a triangular arrangement of the three DNA-binding domains (DBDs) with protein-protein interactions largely mediated by the wing domain. Two structural properties, different flexibility of the wing domain and local DNA conformational changes induced by HSF binding, seem likely to contribute to the subtle differential specificity between HSF1 and HSF2. Besides, two more structures showing DBDs bound to "two-site" head-to-head HSEs were determined as additions to the published tail-to-tail dimer-binding structures.
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http://dx.doi.org/10.1016/j.isci.2021.102951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379338PMC
September 2021

The challenges relating to the effectiveness of augmentation enterocystoplasty for treating ketamine-related bladder contractures.

World J Urol 2021 Aug 26. Epub 2021 Aug 26.

Department of Urology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, China.

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http://dx.doi.org/10.1007/s00345-021-03822-2DOI Listing
August 2021

Biochemical Characterization of a Novel Exo-Type PL7 Alginate Lyase VsAly7D from Marine sp. QY108.

Int J Mol Sci 2021 Aug 5;22(16). Epub 2021 Aug 5.

School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L--5-hexoseulose uronate (DEH) by a non-enzyme reaction, which is an important raw material for the preparation of bioethanol. In our research, a novel exo-type alginate lyase, VsAly7D, belonging to the PL7 family was isolated from marine bacterium sp. QY108 and recombinantly expressed in . The purified VsAly7D demonstrated the highest activity at 35 °C, whereas it still maintained 46.5% and 83.1% of its initial activity at 20 °C and 30 °C, respectively. In addition, VsAly7D exhibited the maximum activity under alkaline conditions (pH 8.0), with the simultaneously remaining stability between pH 8.0 and 10.0. Compared with other reported exo-type enzymes, VsAly7D could efficiently degrade alginate, poly-β-D-mannuronate (polyM) and poly-α-L-guluronate (polyG) with highest specific activities (663.0 U/mg, 913.6 U/mg and 894.4 U/mg, respectively). These results showed that recombinant VsAly7D is a suitable tool enzyme for unsaturated alginate monosaccharide preparation and holds great promise for producing bioethanol from brown algae.
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http://dx.doi.org/10.3390/ijms22168402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395142PMC
August 2021

Determination of Methylene Blue and Its Metabolite Residues in Aquatic Products by High-Performance Liquid Chromatography-Tandem Mass Spectrometry.

Molecules 2021 Aug 17;26(16). Epub 2021 Aug 17.

East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.

A sensitive and reliable method was developed to determine methylene blue (MB) and its metabolite residues, including azure A (AZA), azure B (AZB), and azure C (AZC) in aquatic products by HPLC-MS/MS. The samples were extracted by acetonitrile and cleaned up by alumina-neutral (ALN) cartridges. The analytes were separated on a Sunfire C18 column (150 mm × 2.1 mm, 5 µm). The method was validated according to the European criteria of Commission Decision 2002/657/CE. Good linearity between 1-500 µg/L was obtained with correlation coefficients () greater than 0.99. The limit of quantification (LOQ) was 1.0 µg/kg. The average recoveries at three levels of each compound (1, 5, and 10 µg/kg) were demonstrated to be in the range of 71.8-97.5%, with relative standard deviations (RSDs) from 1.05% to 8.63%. This method was suitable for the detection of methylene blue and its metabolite residues in aquatic products.
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http://dx.doi.org/10.3390/molecules26164975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401997PMC
August 2021

Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression.

Hum Genomics 2021 08 21;15(1):55. Epub 2021 Aug 21.

Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu Province, China.

Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD.
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http://dx.doi.org/10.1186/s40246-021-00354-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379796PMC
August 2021

The Possible Mechanism of Physiological Adaptation to the Low-Se Diet and Its Health Risk in the Traditional Endemic Areas of Keshan Diseases.

Biol Trace Elem Res 2021 Aug 8. Epub 2021 Aug 8.

Department of Nutrition and Metabolism, Chinese Center for Disease Control and Prevention, National Institute for Nutrition and Health, Beijing, 100050, China.

Selenium is an essential trace element for humans and animals. As with oxygen and sulfur, etc., it belongs to the sixth main group of the periodic table of elements. Therefore, the corresponding amino acids, such as selenocysteine (Sec), serine (Ser), and cysteine (Cys), have similar spatial structure, physical, and chemical properties. In this review, we focus on the neglected but key role of serine in a possible mechanism of the physiological adaptation to Se-deficiency in human beings with an adequate intake of dietary protein: the insertion of Cys in place of Sec during the translation of selenoproteins dependent on the Sec insertion sequence element in the 3'UTR of mRNA at the UGA codon through a novel serine-dependent pathway for the de novo synthesis of the Cys-tRNA, similar to Sec-tRNA. We also discuss the important roles of serine in the metabolism of selenium directly or indirectly via GSH, and the maintenance of selenium homostasis regulated through the methylation modification of Sec-tRNA at the position 34U by SAM. Finally, we propose a hypothesis to explain why Keshan disease has gradually disappeared in China and predict the potential health risk of the human body in the physiological adaptation state of low selenium based on the results of animal experiments.
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http://dx.doi.org/10.1007/s12011-021-02851-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349466PMC
August 2021

Identification and Biochemical Characterization of a Surfactant-Tolerant Chondroitinase VhChlABC from LWW-1.

Mar Drugs 2021 Jul 18;19(7). Epub 2021 Jul 18.

School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.

Chondroitinases, catalyzing the degradation of chondroitin sulfate (CS) into oligosaccharides, not only play a crucial role in understanding the structure and function of CS, but also have been reported as a potential candidate drug for the treatment of high CS-related diseases. Here, a marine bacterium LWW-1 was isolated, and its genome was sequenced and annotated. A chondroitinase, VhChlABC, was found to belong to the second subfamily of polysaccharide lyase (PL) family 8. VhChlABC was recombinant expressed and characterized. It could specifically degrade CS-A, CS-B, and CS-C, and reached the maximum activity at pH 7.0 and 40 °C in the presence of 0.25 M NaCl. VhChlABC showed high stability within 8 h under 37 °C and within 2 h under 40 °C. VhChlABC was stable in a wide range of pH (5.0~10.6) at 4 °C. Unlike most chondroitinases, VhChlABC showed high surfactant tolerance, which might provide a good tool for removing extracellular CS proteoglycans (CSPGs) of lung cancer under the stress of pulmonary surfactant. VhChlABC completely degraded CS to disaccharide by the exolytic mode. This research expanded the research and application system of chondroitinases.
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http://dx.doi.org/10.3390/md19070399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306027PMC
July 2021

Simultaneous Recognition of Atrophic Gastritis and Intestinal Metaplasia on White Light Endoscopic Images Based on Convolutional Neural Networks: A Multicenter Study.

Clin Transl Gastroenterol 2021 08 3;12(8):e00385. Epub 2021 Aug 3.

Department of Gastroenterology, Sir Run Run Shaw Hospital, Medical School, Zhejiang University, Hangzhou, China.

Introduction: Patients with atrophic gastritis (AG) or gastric intestinal metaplasia (GIM) have elevated risk of gastric adenocarcinoma. Endoscopic screening and surveillance have been implemented in high incidence countries. The study aimed to evaluate the accuracy of a deep convolutional neural network (CNN) for simultaneous recognition of AG and GIM.

Methods: Archived endoscopic white light images with corresponding gastric biopsies were collected from 14 hospitals located in different regions of China. Corresponding images by anatomic sites containing AG, GIM, and chronic non-AG were categorized using pathology reports. The participants were randomly assigned (8:1:1) to the training cohort for developing the CNN model (TResNet), the validation cohort for fine-tuning, and the test cohort for evaluating the diagnostic accuracy. The area under the curve (AUC), sensitivity, specificity, and accuracy with 95% confidence interval (CI) were calculated.

Results: A total of 7,037 endoscopic images from 2,741 participants were used to develop the CNN for recognition of AG and/or GIM. The AUC for recognizing AG was 0.98 (95% CI 0.97-0.99) with sensitivity, specificity, and accuracy of 96.2% (95% CI 94.2%-97.6%), 96.4% (95% CI 94.8%-97.9%), and 96.4% (95% CI 94.4%-97.8%), respectively. The AUC for recognizing GIM was 0.99 (95% CI 0.98-1.00) with sensitivity, specificity, and accuracy of 97.9% (95% CI 96.2%-98.9%), 97.5% (95% CI 95.8%-98.6%), and 97.6% (95% CI 95.8%-98.6%), respectively.

Discussion: CNN using endoscopic white light images achieved high diagnostic accuracy in recognizing AG and GIM.
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http://dx.doi.org/10.14309/ctg.0000000000000385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337066PMC
August 2021

Intercalation of Thin-Film Gd-Doped Ceria Barrier Layers in Electrolyte-Supported Solid Oxide Cells: Physicochemical Aspects.

ACS Appl Mater Interfaces 2021 Aug 28;13(31):37239-37251. Epub 2021 Jul 28.

German Aerospace Centre (DLR), Institute of Engineering Thermodynamics, Pfaffenwaldring 38-40, Stuttgart 70569, Germany.

To minimize alteration of the LaSrCoFeO (LSCF)/GdCeO(CGO20)/YZrO(3YSZ) interface via strontium zirconate formation in solid oxide cells, electron beam physical vapor deposition was employed to manufacture dense, thin gadolinium-doped ceria (CGO) interlayers. CGO layers with thicknesses of 0.15, 0.3, and 0.5 μm were integrated in state-of-the-art 5 × 5 cm-large electrolyte-supported cells, and their performance characteristics and degradation behavior were investigated. Electrochemical impedance spectroscopy measurements are correlated with a postmortem scanning electron microscopy/energy-dispersive X-ray spectroscopy analysis to show that 0.15 μm-thick layers lead to the formation of a continuous Sr-containing secondary phase at the CGO/YSZ interface, likely related to the formation of a SrO-ZrO phase. Major performance losses were confirmed by an increase in both Ohmic and polarization resistance with an increase in the frequency region ∼10 Hz. Cells with 0.3 μm- and 0.5 μm-thick CGO layers showed similar high performance and low degradation rates over a testing period of ∼800 h. The YSZ/CGO interface of the cells with a 0.3 μm-thick CGO layer showed the formation of a discontinuous Sr-containing secondary phase; however, performance losses were still successfully prevented. Furthermore, it is observed that 0.5 μm-thick CGO layers were sufficient to suppress the formation of the Sr-containing secondary phase.
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http://dx.doi.org/10.1021/acsami.1c11175DOI Listing
August 2021

The Association Between Serum Endocan Level and Short-Term Prognosis of Patients With Acute Ischemic Stroke.

Angiology 2021 Jul 16:33197211030732. Epub 2021 Jul 16.

Clinical Medical College Jiujiang University Hospital, 71220Jiujiang University, Jiujiang, China.

This cohort study was designed to assess the association between serum endocan levels and the prognosis of acute ischemic stroke. A total of 227 patients were recruited consecutively. Study outcome data on death and major disability (modified Rankin Scale score ≥3) were collected at 3 months after stroke onset. After 3 months of follow-up, death and disability occurred in 48 and 85 patients, respectively, while the primary (death) and secondary (death or disability) outcome incident rate was 21.15% and 37.44%, respectively. The multivariable adjusted odds ratio (OR) (95% confidence interval, 95% CIs) of the highest endocan quartile for death or major disability was 1.21 (1.10, 4.13) compared with the lowest quartile. After adjusting for confounding factors, the increase in the risk of death was not significant. Receiver operating characteristic curve analysis showed that endocan predicted primary and secondary outcomes with C-statistical values (95% CIs) of 0.61 (0.55-0.67, = .001) and 0.68 (0.59-0.76, < .001), respectively. Elevated endocan levels were independently related to increased risk of poor outcome at 3 months after ischemic stroke onset. Endocan is a potential prognostic factor for ischemic stroke.
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http://dx.doi.org/10.1177/00033197211030732DOI Listing
July 2021

Special issue: Neuroinflammatory pathways as treatment targets in brain disorders autophagic regulation of neuroinflammation in ischemic stroke.

Neurochem Int 2021 09 27;148:105114. Epub 2021 Jun 27.

Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address:

Despite the high lethality and increasing prevalence, effective therapy for ischemic stroke is still limited. As a crucial pathophysiological mechanism underlying ischemic injury, neuroinflammation remains a promising target for novel anti-ischemic strategies. However, the potential adverse effects limit the applications of traditional anti-inflammatory therapies. Recent explorations into the mechanisms of inflammation reveal that autophagy acts as a critical part in inflammation regulation. Autophagy refers to the hierarchically organized process resulting in the lysosomal degradation of intracellular components. Autophagic clearance of intracellular danger signals (DAMPs) suppresses the inflammation activation. Alternatively, autophagy blunts inflammation by removing either inflammasomes or the transcriptional modulators of cytokines. Interestingly, several compounds have been proved to alleviate neuroinflammatory responses and protect against ischemic injury by activating autophagy, highlighting autophagy as a promising target for the regulation of ischemia-induced neuroinflammation. Nonetheless, the molecular mechanism underlying autophagic regulation of neuroinflammation in the central nervous system is less clear and further explorations are still needed.
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http://dx.doi.org/10.1016/j.neuint.2021.105114DOI Listing
September 2021

Characterization of a Hyaluronic Acid Utilization Locus and Identification of Two Hyaluronate Lyases in a Marine Bacterium LWW-9.

Front Microbiol 2021 10;12:696096. Epub 2021 Jun 10.

Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China.

Hyaluronic acid (HA) is a negatively charged and linear polysaccharide existing in the tissues and body fluids of all vertebrates. Some pathogenic bacteria target hyaluronic acid for adhesion and/or infection to host cells. is an opportunistic pathogen related to infections of humans and marine animals, and the hyaluronic acid-degrading potential of spp. has been well-demonstrated. However, little is known about how spp. utilize hyaluronic acid. In this study, a marine bacterium LWW-9 capable of degrading hyaluronic acid has been isolated. Genetic and bioinformatic analysis showed that LWW-9 harbors a gene cluster involved in the degradation, transport, and metabolism of hyaluronic acid. Two novel PL8 family hyaluronate lyases, VaHly8A and VaHly8B, are the key enzymes for the degradation of hyaluronic acid. VaHly8A and VaHly8B have distinct biochemical properties, reflecting the adaptation of the strain to the changing parameters of the aquatic habitats and hosts. Based on genomic and functional analysis, we propose a model for the complete degradation of hyaluronic acid by LWW-9. Overall, our study expands our knowledge of the HA utilization paradigm within the , and the two novel hyaluronate lyases are excellent candidates for industrial applications.
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http://dx.doi.org/10.3389/fmicb.2021.696096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222515PMC
June 2021

Regulation of the Volume Flow Rate of Aqueous Methyl Blue Solution and the Wettability of CuO/ZnO Nanorods to Improve the Photodegradation Performance of Related Microfluidic Reactors.

Langmuir 2021 07 21;37(26):7890-7906. Epub 2021 Jun 21.

State Key Laboratory for Manufacturing Systems Engineering at Xi'an Jiaotong University, Xi'an 710049, P. R. China.

Six CuO/ZnO nanorod (CuO/ZnONR)-based microfluidic reactors were constructed for different UV irradiation durations, with which an aqueous methylene blue (MB) solution was photodegraded at varied volume flow rate . Via numerical and experimental routes, the effects of the on the kinetic adsorption rate constant and the initial rate constant of the CuO/ZnONR-based microfluidic reactors were discussed. Moreover, a reverse contacting angle (CA) trend of CuO/ZnONRs to the reaction constant curve of corresponding CuO/ZnONR-based microfluidic reactor suggested that the CA of CuO/ZnONRs was another key influencing factor that affected greatly the photodegradation performance of the microfluidic reactors. The of the aqueous MB solution and the UV irradiation duration for the photodeposition of CuO/ZnONRs were optimized to be 125 μL/min and 1.0 h, the of the CuO/ZnONR-based microfluidic reactors reached 4.84 min, and the related / was less than 6%. Similarly, these methods and results can be employed not only to enhance the mass transport and adsorption of specific species within other nanostructured matrix material-coated microchannels but also to enlarge the actual contacting surface areas between these microchannels and the related solution, which further improve the performance of other nanostructured catalyst-based microfluidic reactors, rGO microfluidic voltage generation, and a GOx/AuNW enzymatic glucose microfluidic sensor.
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http://dx.doi.org/10.1021/acs.langmuir.1c00407DOI Listing
July 2021

Continuous-wave near-infrared stimulated-emission depletion microscopy using downshifting lanthanide nanoparticles.

Nat Nanotechnol 2021 09 14;16(9):975-980. Epub 2021 Jun 14.

Department of Chemistry, National University of Singapore, Singapore, Singapore.

Stimulated-emission depletion (STED) microscopy has profoundly extended our horizons to the subcellular level. However, it remains challenging to perform hours-long, autofluorescence-free super-resolution imaging in near-infrared (NIR) optical windows under facile continuous-wave laser depletion at low power. Here we report downshifting lanthanide nanoparticles that enable background-suppressed STED imaging in all-NIR spectral bands (λ = 808 nm, λ = 1,064 nm and λ = 850-900 nm), with a lateral resolution of below 20 nm and zero photobleaching. With a quasi-four-level configuration and long-lived (τ > 100 μs) metastable states, these nanoparticles support near-unity (98.8%) luminescence suppression under 19 kW cm saturation intensity. The all-NIR regime enables high-contrast deep-tissue (~50 μm) imaging with approximately 70 nm spatial resolution. These lanthanide nanoprobes promise to expand the application realm of STED microscopy and pave the way towards high-resolution time-lapse investigations of cellular processes at superior spatial and temporal dimensions.
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http://dx.doi.org/10.1038/s41565-021-00927-yDOI Listing
September 2021

Snapshot polarized light scattering spectroscopy using spectrally-modulated polarimetry for early gastric cancer detection.

J Biophotonics 2021 09 17;14(9):e202100140. Epub 2021 Jun 17.

Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Polarized light scattering spectroscopy (PLSS) is a promising optical technique developed for the detection of cancer, which extracts the single scattering light to infer morphological information of epithelial cells. However, traditional PLSS uses either a rotatable polarizer or two orthogonal polarizers to purify the single scattering light, which makes it complicated and challenged to build a PLSS endoscope. Herein, we propose a snapshot PLSS with a single optical path to directly get the single scattering light for the first time. The single scattering light is encoded using the spectrally-modulated polarimetry and decoded using the continuous slide iterative method. Both the polystyrene microsphere solutions and the ex vivo gastric cancer samples are used to verify the method. The experimental results of the snapshot PLSS are consistent well with that of the traditional PLSS. The proposed method has a potential for the building of snapshot PLSS endoscope systems in future.
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http://dx.doi.org/10.1002/jbio.202100140DOI Listing
September 2021

Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice.

Biomedicines 2021 May 10;9(5). Epub 2021 May 10.

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aramaki-Aoba, Aoba-Ku, Sendai 980-8578, Japan.

Fatty acid-binding proteins (FABPs) regulate the intracellular dynamics of fatty acids, mediate lipid metabolism and participate in signaling processes. However, the therapeutic efficacy of targeting FABPs as novel therapeutic targets for cerebral ischemia is not well established. Previously, we synthesized a novel FABP inhibitor, i.e., FABP ligand 6 [4-(2-(5-(2-chlorophenyl)-1-(4-isopropylphenyl)-1H-pyrazol-3-yl)-4-fluorophenoxy)butanoic acid] (referred to here as MF6). In this study, we analyzed the ability of MF6 to ameliorate transient middle cerebral artery occlusion (tMCAO) and reperfusion-induced injury in mice. A single MF6 administration (3.0 mg/kg, per os) at 0.5 h post-reperfusion effectively reduced brain infarct volumes and neurological deficits. The protein-expression levels of FABP3, FABP5 and FABP7 in the brain gradually increased after tMCAO. Importantly, MF6 significantly suppressed infarct volumes and the elevation of FABP-expression levels at 12 h post-reperfusion. MF6 also inhibited the promotor activity of FABP5 in human neuroblastoma cells (SH-SY5Y). These data suggest that FABPs elevated infarct volumes after ischemic stroke and that inhibiting FABPs ameliorated the ischemic injury. Moreover, MF6 suppressed the inflammation-associated prostaglandin E levels through microsomal prostaglandin E synthase-1 expression in the ischemic hemispheres. Taken together, the results imply that the FABP inhibitor MF6 can potentially serve as a neuroprotective therapeutic for ischemic stroke.
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http://dx.doi.org/10.3390/biomedicines9050529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150391PMC
May 2021

Reduced coupling between cerebrospinal fluid flow and global brain activity is linked to Alzheimer disease-related pathology.

PLoS Biol 2021 06 1;19(6):e3001233. Epub 2021 Jun 1.

Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania, United States of America.

The glymphatic system plays an important role in clearing the amyloid-β (Aβ) and tau proteins that are closely linked to Alzheimer disease (AD) pathology. Glymphatic clearance, as well as Aβ accumulation, is highly dependent on sleep, but the sleep-dependent driving forces behind cerebrospinal fluid (CSF) movements essential to the glymphatic flux remain largely unclear. Recent studies have reported that widespread, high-amplitude spontaneous brain activations in the drowsy state and during sleep, which are shown as large global signal peaks in resting-state functional magnetic resonance imaging (rsfMRI), are coupled with CSF movements, suggesting their potential link to glymphatic flux and metabolite clearance. By analyzing multimodal data from the Alzheimer's Disease Neuroimaging Initiative (ADNI) project, here we showed that the coupling between the global fMRI signal and CSF influx is correlated with AD-related pathology, including various risk factors for AD, the severity of AD-related diseases, the cortical Aβ level, and cognitive decline over a 2-year follow-up. These results provide critical initial evidence for involvement of sleep-dependent global brain activity, as well as the associated physiological modulations, in the clearance of AD-related brain waste.
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http://dx.doi.org/10.1371/journal.pbio.3001233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168893PMC
June 2021

Effect of , -Dimethylglycine on Homocysteine Metabolism in Rats Fed Folate-Sufficient and Folate-Deficient Diets.

Biomed Environ Sci 2021 May;34(5):356-363

Key Laboratory of Trace Element Nutrition of National Health Commission, National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: This study aimed to investigate the effects of -dimethylglycine (DMG) on the concentration and metabolism of plasma homocysteine (pHcy) in folate-sufficient and folate-deficient rats.

Methods: In this study, 0.1% DMG was supplemented in 20% casein diets that were either folate-sufficient (20C) or folate-deficient (20CFD). Blood and liver of rats were subjected to assays of Hcy and its metabolites. Hcy and its related metabolite concentrations were determined using a liquid chromatographic system.

Results: Folate deprivation significantly increased pHcy concentration in rats fed 20C diet (from 14.19 ± 0.39 μmol/L to 28.49 ± 0.50 μmol/L; < 0.05). When supplemented with DMG, pHcy concentration was significantly decreased (12.23 ± 0.18 μmol/L) in rats fed 20C diet but significantly increased (31.56 ± 0.59 μmol/L) in rats fed 20CFD. The hepatic methionine synthase activity in the 20CFD group was significantly lower than that in the 20C group; enzyme activity was unaffected by DMG supplementation regardless of folate sufficiency. The activity of hepatic cystathionine β-synthase (CBS) in the 20CFD group was decreased but not in the 20C group; DMG supplementation enhanced hepatic CBS activity in both groups, in which the effect was significant in the 20C group but not in the other group.

Conclusion: DMG supplementation exhibited hypohomocysteinemic effects under folate-sufficient conditions. By contrast, the combination of folate deficiency and DMG supplementation has deleterious effect on pHcy concentration.
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http://dx.doi.org/10.3967/bes2021.047DOI Listing
May 2021

Efficient Construction of 5H-1,4-Benzodiazepine Derivatives by a Catalyst-Free Direct Aerobic Oxidative Annulation Strategy.

ChemSusChem 2021 Jul 18;14(14):2866-2871. Epub 2021 Jun 18.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, P. R. China.

A catalyst-free direct aerobic oxidative annulation reaction of 2-aminobenzylic amines and α-hydroxy ketones efficiently afforded versatile 5H-1,4-benzodiazepine derivatives by employing air as economic and green oxidant under mild conditions. Interestingly, solvent was found to be crucial to the reaction, so that by using acetic acid as the best solvent an efficient and practical method could be achieved, requiring no catalysts or additives at all. This method tolerates a wide range of 2-aminobenzylic amines and α-hydroxy ketones and could be scaled up to multigram synthesis and directly applied in one-step synthesis of the pharmaceutically active N-desmethylmedazepam derivatives, revealing the potential of this new method in the synthesis of 5H-1,4-benzodiazepine skeleton-based pharmaceuticals and chemicals.
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http://dx.doi.org/10.1002/cssc.202100703DOI Listing
July 2021
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