Publications by authors named "Feng Gao"

3,787 Publications

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Physics of defects in metal halide perovskites.

Rep Prog Phys 2022 Jun 28. Epub 2022 Jun 28.

Department of Physics, Chemistry, and Biology, Linköping University, F-House, Valla, Linköping, 581 83, SWEDEN.

Metal halide perovskites are widely used in optoelectronic devices, including solar cells, photodetectors, and light-emitting diodes. Defects in this class of low-temperature and solution-processed semiconductors play significant roles in the optoelectronic properties and performance of devices based on these semiconductors. Investigating the defect properties provides not only insight into the origin of the outstanding performance of perovskite optoelectronic devices but also guidance for further improvement of performance. Defects in perovskites have been intensely studied. Here, we review the progress in defect-related physics and techniques for perovskites. We survey the theoretical and computational results of the origin and properties of defects in perovskites. The underlying mechanism, functions, advantages, and limitations of trap state characterization techniques are discussed. We introduce the effect of defects on the performance of perovskite optoelectronic devices, followed by a discussion of the mechanism of defect treatment. Finally, we summarize and present key challenges and opportunities of defects and their role in the further development of perovskite optoelectronic devices.
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http://dx.doi.org/10.1088/1361-6633/ac7c7aDOI Listing
June 2022

Sequence-Dependent Tyrosine-Containing Peptide Nanoassemblies for Sensing Tyrosinase and Melanoma.

ACS Macro Lett 2022 Jun 28:875-881. Epub 2022 Jun 28.

Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer-Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Tyrosine-containing peptide nanoassemblies have received tremendous attention because of their potential applications in biomedicine and nanomaterial fields. However, a current outstanding challenge is to direct the equilibrium between oxidative polymerization of precursors and the noncovalent assembly to precisely tune their specific nanostructures and functionalities through the rational design of peptide sequences. With a simple library of tripeptides containing tyrosine, glycine, and lysine, here we demonstrate how amino acid sequence encodes the property of tripeptide nanoassemblies by modulating the enzymatic oxidation of tyrosinase with the accompanied self-assembly, and thus select the pathways toward fluorescent or melanin-like nanoassemblies. The fluorescence of tripeptide nanoassemblies has been demonstrated in sensing both tyrosinase and melanoma. Our findings will provide inspiration of peptide sequence design for generating the complex bioactive peptide nanomaterials for biomedical applications.
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http://dx.doi.org/10.1021/acsmacrolett.2c00180DOI Listing
June 2022

PHEV infection: A promising model of betacoronavirus-associated neurological and olfactory dysfunction.

PLoS Pathog 2022 Jun 27;18(6):e1010667. Epub 2022 Jun 27.

State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurotropic coronavirus belonging to the genus Betacoronavirus. Similar to pathogenic coronaviruses to which humans are susceptible, such as SARS-CoV-2, PHEV is transmitted primarily through respiratory droplets and close contact, entering the central nervous system (CNS) from the peripheral nerves at the site of initial infection. However, the neuroinvasion route of PHEV are poorly understood. Here, we found that BALB/c mice are susceptible to intranasal PHEV infection and showed distinct neurological manifestations. The behavioral study and histopathological examination revealed that PHEV attacks neurons in the CNS and causes significant smell and taste dysfunction in mice. By tracking neuroinvasion, we identified that PHEV invades the CNS via the olfactory nerve and trigeminal nerve located in the nasal cavity, and olfactory sensory neurons (OSNs) were susceptible to viral infection. Immunofluorescence staining and ultrastructural observations revealed that viral materials traveling along axons, suggesting axonal transport may engage in rapid viral transmission in the CNS. Moreover, viral replication in the olfactory system and CNS is associated with inflammatory and immune responses, tissue disorganization and dysfunction. Overall, we proposed that PHEV may serve as a potential prototype for elucidating the pathogenesis of coronavirus-associated neurological complications and olfactory and taste disorders.
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http://dx.doi.org/10.1371/journal.ppat.1010667DOI Listing
June 2022

Prediction of the generalization of myasthenia gravis with purely ocular symptoms at onset: a multivariable model development and validation.

Ther Adv Neurol Disord 2022 21;15:17562864221104508. Epub 2022 Jun 21.

Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Background: About half of myasthenia gravis (MG) patients with purely ocular symptoms at onset progress to generalized myasthenia gravis (gMG).

Objectives: To develop and validate a model to predict the generalization of MG at 6 months after disease onset in patients with ocular-onset myasthenia gravis (OoMG).

Methods: Data of patients with OoMG were retrospectively collected from two tertiary hospitals in Germany and China. An accelerated failure time model was developed using the backward elimination method based on the German cohort to predict the generalization of OoMG. The model was then externally validated in the Chinese cohort, and its performance was assessed using Harrell's C-index and calibration plots.

Results: Four hundred and seventy-seven patients (275 from Germany and 202 from China) were eligible for inclusion. One hundred and three (37.5%) patients in the German cohort progressed from OoMG to gMG with a median follow-up time of 69 (32-116) months. The median time to generalization was 29 (16-71) months. The estimated cumulative probability of generalization was 30.5% [95% CI (confidence interval), 24.3-36.2%) at 5 years after disease onset. The final model, which was represented as a nomogram, included five clinical variables: sex, titer of anti-AChR antibody, status of anti-MuSK antibody, age at disease onset and the presence of other autoimmune disease. External validation of the model using the bootstrap showed a C-index of 0.670 (95% CI, 0.602-0.738). Calibration curves revealed moderate agreement of predicted and observed outcomes.

Conclusion: The nomogram is a good predictor for generalization in patients with OoMG that can be used to inform of the individual generalization risk, which might improve the clinical decision-making.
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http://dx.doi.org/10.1177/17562864221104508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218496PMC
June 2022

Statistical estimation and comparison of group-specific bivariate correlation coefficients in family-type clustered studies.

J Appl Stat 2022 18;49(9):2246-2270. Epub 2021 Mar 18.

Department of Neurology, Alzheimer Disease Research Center, Washington University School of Medicine, St. Louis, MO, USA.

Bivariate correlation coefficients (BCCs) are often calculated to gauge the relationship between two variables in medical research. In a family-type clustered design where multiple participants from same units/families are enrolled, BCCs can be defined and estimated at various hierarchical levels (subject level, family level and marginal BCC). Heterogeneity usually exists between subject groups and, as a result, subject level BCCs may differ between subject groups. In the framework of bivariate linear mixed effects modeling, we define and estimate BCCs at various hierarchical levels in a family-type clustered design, accommodating subject group heterogeneity. Simplified and modified asymptotic confidence intervals are constructed to the BCC differences and Wald type tests are conducted. A real-world family-type clustered study of Alzheimer disease (AD) is analyzed to estimate and compare BCCs among well-established AD biomarkers between mutation carriers and non-carriers in autosomal dominant AD asymptomatic individuals. Extensive simulation studies are conducted across a wide range of scenarios to evaluate the performance of the proposed estimators and the type-I error rate and power of the proposed statistical tests. Abbreviations: BCC: bivariate correlation coefficient; BLM: bivariate linear mixed effects model; CI: confidence interval; AD: Alzheimer's disease; DIAN: The Dominantly Inherited Alzheimer Network; SA: simple asymptotic; MA: modified asymptotic.
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http://dx.doi.org/10.1080/02664763.2021.1899141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225315PMC
March 2021

Electrochemically classifying DNA structure based on the small molecule-DNA recognition.

Bioelectrochemistry 2022 Jun 17;147:108193. Epub 2022 Jun 17.

Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, PR China. Electronic address:

Herein, we reported the differential binding ability of aminoglycosides to DNA structures using electrochemical method through principal component analysis (PCA) to classify different DNA secondary structures and understand the link between secondary structure and DNA conformation. In these analyses, the DNA with different secondary structure motifs: bulge, internal loop, hairpin loop and stem loop were designed. The aminoglycosides as receptors were modified on the surface of electrode. In the presence of DNA, the DNA will be absorbed on the surface of electrode via the recognition of DNA and aminoglycosides, resulting in the electrochemical signal observed in [Fe(CN)]. Furthermore, the DNA structures labeled with 2-aminopurine (2-AP) at the structural motif of interest were also employed to study the binding affinity between aminoglycosides and different DNA motifs. The PCA suggested that this method may achieve nucleotide-specific classification of two independent secondary structure motifs, and the structure and sequence of DNA and the size and structure of small molecule could affect the binding ability of the aminoglycosides and DNA. This approach presents a new approach to classify DNA structure and offers ideas for designing targeted drugs small molecule compounds for wound dressing and drug delivery.
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http://dx.doi.org/10.1016/j.bioelechem.2022.108193DOI Listing
June 2022

The Xiangya equation could not replace the Asian modified CKD-EPI equation in estimating glomerular filtration rate in Chinese patients with chronic kidney disease.

Int Urol Nephrol 2022 Jun 26. Epub 2022 Jun 26.

Department of Nuclear Medicine, The Third Hospital of Hebei Medical University, No.139, ZiQiang Road, Shijiazhuang, 050051, Hebei Province, People's Republic of China.

Background: To evaluate the clinical practicability of the Xiangya equation in estimating glomerular filtration rate (GFR) and compare with the Asian modified Chronic Kidney Disease-Epidemiology Collaboration (CKD-EPI) equation, assessing whether the Xiangya equation could replace the Asian modified CKD-EPI equation as the preferred method for predicting GFR in the Chinese CKD patients in an external validation study.

Methods: The GFR was determined simultaneously by two methods: (a) the Asian modified CKD-EPI equation (GFR); (b) the Xiangya equation (GFR); diagnostic performance of the two models was compared by the regression analysis, the Bland-Altman plot, bias, precision and P under the background of Tc-diethylenetriaminepentaacetic acid (Tc-DTPA) dual plasma sample clearance method as reference method for GFR measurement (mGFR).

Results: A total of 158 Chinese CKD patients were included in our external study. The GFR was highly related with mGFR, with the correlation coefficient of 0.92. The regression equation was GFR = 0.55*mGFR + 28.25, where the regression coefficient was far away from one and the intercept was wide. Compared with the Asian modified CKD-EPI equation, the performance of the Xiangya equation demonstrated a poorer bias (9.5 vs - 3.3 ml/min/1.73 m, P < 0.001), an inferior precision (23.9 vs 13.0 ml/min/1.73 m, P < 0.001), a lower P (51.3% vs 73.4%, P < 0.001) and a wider 95% limit of agreement in Bland-Altman analysis (65.0 vs 44.9 ml/min/1.73 m).

Conclusion: Due to relatively inferior performance, the Xiangya equation could not replace the Asian modified CKD-EPI equation in estimating GFR in Chinese patients with chronic kidney disease in our external cohort.
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http://dx.doi.org/10.1007/s11255-022-03248-8DOI Listing
June 2022

Gut Microbiota Combined with Metabolites Reveals Unique Features of Acute Myocardial Infarction Patients Different from Stable Coronary Artery Disease.

J Adv Res 2022 Jun 21. Epub 2022 Jun 21.

State Key Laboratory for Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Introduction: Acute myocardial infarction (AMI) accounts for the majority of deaths caused by coronary artery disease (CAD). Early warning of AMI, especially for patients with stable coronary artery disease (sCAD), is urgently needed. Our previous study showed that alterations in the gut microbiota were correlated with CAD severity.

Objectives: Herein, we tried to discover accurate and convenient biomarkers for AMI by combination of gut microbiota and fecal/blood/urinary metabolomics.

Methods: We recruited 190 volunteers including 93 sCAD patients, 49 AMI patients, and 48 subjects with normal coronary artery (NCA), and measured their blood biochemical parameters, 16S rRNA-based gut microbiota and NMR-based fecal/blood/urinary metabolites. We further selected 20 subjects from each group and analyzed their gut microbiota by whole-metagenome shotgun sequencing.

Results: Multi-omic analyses revealed that AMI patients exhibited specific changes in gut microbiota and serum/urinary/fecal metabolites as compared to subjects with sCAD or NCA. Fourteen bacterial genera and 30 metabolites (11 in feces, 10 in blood, 9 in urine) were closely related to AMI phenotypes and could accurately distinguish AMI patients from sCAD patients. Some species belonging to Alistipes, Streptococcus, Ruminococcus, Lactobacillus and Faecalibacterium were effective to distinguish AMI from sCAD and their predictive ability was confirmed in an independent cohort of CAD patients. We further selected nine indicators including 4 bacterial genera, 3 fecal and 2 urinary metabolites as a noninvasive biomarker set which can distinguish AMI from sCAD with an AUC of 0.932.

Conclusion: Combination of gut microbiota and fecal/urinary metabolites provided a set of potential useful and noninvasive predictive biomarker for AMI from sCAD.
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http://dx.doi.org/10.1016/j.jare.2022.06.008DOI Listing
June 2022

Palladium-catalyzed stereoselective ring-opening reaction of aryl cyclopropyl ketones.

Org Biomol Chem 2022 Jun 24. Epub 2022 Jun 24.

Department of Pharmacy, The First Afflicted Hospital of Chengdu Medical College, Chengdu 610500, PR China.

Herein, we report that α,β-unsaturated ketones could be obtained by palladium-catalyzed ring-opening of mono-substituted cyclopropyl ketones efficiently and systematically. ()-1-Arylbut-2-en-1-ones were generated from aryl cyclopropyl ketones stereoselectively in yields of 23-89% by the Pd(OAc)/PCy catalytic system. The reaction exhibited stereoselectivity (only products were found) and was suitable for both phenyl and heteroaryl cyclopropyl ketones.
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http://dx.doi.org/10.1039/d2ob00719cDOI Listing
June 2022

Tuning Multicolor Emission of Manganese-Activated Gallogermanate Nanophosphors by Regulating Mn Ions Occupying Sites for Multiple Anti-Counterfeiting Application.

Nanomaterials (Basel) 2022 Jun 13;12(12). Epub 2022 Jun 13.

Department of Physics, The University of Texas at Arlington, Arlington, TX 76019-0059, USA.

The ability to manipulate the luminescent color, intensity and long lifetime of nanophosphors is important for anti-counterfeiting applications. Unfortunately, persistent luminescence materials with multimode luminescent features have rarely been reported, even though they are expected to be highly desirable in sophisticated anti-counterfeiting. Here, the luminescence properties of ZnGaGeO:Mn phosphors were tuned by using different preparation approaches, including a hydrothermal method and solid-state reaction approach combining with non-equivalent ion doping strategy. As a result, Mn-activated ZnGaGeO phosphors synthesized by a hydrothermal method demonstrate an enhanced red photoluminescence at 701 nm and a strong green luminescence with persistent luminescence and photostimulated luminescence at 540 nm. While Mn-activated ZnGaGeO phosphors synthesized by solid-state reactions combined with a hetero-valent doping approach only exhibit an enhanced single-band red emission. Keeping the synthetic method unchanged, the substitution of hetero-valent dopant ion Li into different sites is valid for spectral fine-tuning. A spectral tuning mechanism is also proposed. Mn-activated ZnGaGeO phosphors synthesized by a hydrothermal approach with multimodal luminescence is especially suitable for multiple anti-counterfeiting, multicolor display and other potential applications.
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http://dx.doi.org/10.3390/nano12122029DOI Listing
June 2022

Experimental Study on the Dry Drilling Nickel-Based Superalloy of CrAlYN Coated Carbide Bit.

Materials (Basel) 2022 Jun 17;15(12). Epub 2022 Jun 17.

School of Materials Science and Engineering, Xi'an University of Technology, Xi'an 710048, China.

Nickel-based superalloy is regarded as one of the materials with the poorest cutting and drilling performance. Additionally, there is much less research on the drilling of it. This paper aims to study the drilling performance of dry drilling nickel-based superalloy with uncoated and CrAlYN coated carbide bit. First of all, the primary and secondary factors influencing the machining performance of dry drilling nickel-base superalloy uncoated carbide bit were explored through an orthogonal test. Secondly, the self-prepared CrAlYN coated carbide drills, and uncoated drills were compared and analyzed from perspectives of service life, drilling force, drilling temperature, drill surface topography, failure mechanism, and machining surface quality. The research results are as follows: the drilling temperature is the primary factor affecting the drilling performance under dry drilling conditions. CrAlYN coating can obviously prolong the service life of tools, reduce the drilling force and drilling temperature, and improve the machining surface quality at lower rotational speeds. Moreover, the coated cemented carbide bit has a similar failure mode to the uncoated cemented carbide bit after the CrAlYN coating falls off in the wear zone of cemented carbide bit, which is mainly bonding wear on the rear tool surface and the front tool surface.
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http://dx.doi.org/10.3390/ma15124302DOI Listing
June 2022

Strategies for Controlling or Releasing the Influence Due to the Volume Expansion of Silicon inside Si-C Composite Anode for High-Performance Lithium-Ion Batteries.

Materials (Basel) 2022 Jun 16;15(12). Epub 2022 Jun 16.

College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China.

Currently, silicon is considered among the foremost promising anode materials, due to its high capacity, abundant reserves, environmental friendliness, and low working potential. However, the huge volume changes in silicon anode materials can pulverize the material particles and result in the shedding of active materials and the continual rupturing of the solid electrolyte interface film, leading to a short cycle life and rapid capacity decay. Therefore, the practical application of silicon anode materials is hindered. However, carbon recombination may remedy this defect. In silicon/carbon composite anode materials, silicon provides ultra-high capacity, and carbon is used as a buffer, to relieve the volume expansion of silicon; thus, increasing the use of silicon-based anode materials. To ensure the future utilization of silicon as an anode material in lithium-ion batteries, this review considers the dampening effect on the volume expansion of silicon particles by the formation of carbon layers, cavities, and chemical bonds. Silicon-carbon composites are classified herein as coated core-shell structure, hollow core-shell structure, porous structure, and embedded structure. The above structures can adequately accommodate the Si volume expansion, buffer the mechanical stress, and ameliorate the interface/surface stability, with the potential for performance enhancement. Finally, a perspective on future studies on Si-C anodes is suggested. In the future, the rational design of high-capacity Si-C anodes for better lithium-ion batteries will narrow the gap between theoretical research and practical applications.
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http://dx.doi.org/10.3390/ma15124264DOI Listing
June 2022

Fluorinated Perylene-diimides: Cathode Interlayers Facilitating Carrier Collection for High-Performance Organic Solar Cells.

Adv Mater 2022 Jun 21:e2203690. Epub 2022 Jun 21.

Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Organic solar cells (OSCs) have experienced rapid progress with the innovation of near-infrared (NIR)-absorbing small-molecular acceptors (SMAs), while the unique electronic properties of the SMAs raise new challenges in relation to cathode engineering for effective electron collection. To address this issue, we synthesized two fluorinated perylene-diimides (PDIs), PDINN-F and PDINN-2F by a simple fluorination method, for the application as cathode interlayer (CIL) materials. The two bay-fluorinated PDI based CILs possess lower the lowest unoccupied molecular orbital energy level of ca. -4.0 eV, which improves the energy level alignment at the NIR-SMAs (such as BTP-eC9)/cathode interface for a favorable electron extraction efficiency. The mono-fluorinated PDINN-F shows higher electron mobility and better improved interfacial compatibility. The PDINN-F based OSCs with PM6: BTP-eC9 as active layer exhibit an enhanced fill factor and larger short-circuit current density, leading to a high power conversion efficiency (PCE) exceeding 18%. The devices with PDINN-F CIL retained more than 80% of its initial PCE after operating at the maximum power point under continuous illumination for 750 hours. This work prescribes a facile, cost-effective and scalable method for the preparation of stable, high-performance fluorinated CILs, and instilling promise for the NIR-SMAs-based OSCs moving forward. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202203690DOI Listing
June 2022

The feasibility of proteomics sequencing based immune-related prognostic signature for predicting clinical outcomes of bladder cancer patients.

BMC Cancer 2022 Jun 20;22(1):676. Epub 2022 Jun 20.

Pathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100, Hai Ning Road, Shanghai, 200080, China.

Background: Bladder cancer (BCa) shows its potential immunogenity in current immune-checkpoint inhibitor related immunotherapies. However, its therapeutic effects are improvable and could be affected by tumor immune microenvironment. Hence it is interesting to find some more prognostic indicators for BCa patients concerning immunotherapies.

Methods: In the present study, we retrospect 129 muscle-invasive BCa (MIBC) patients with radical cystectomy in Shanghai General Hospital during 2007 to 2018. Based on the results of proteomics sequencing from 9 pairs of MIBC tissue from Shanghai General Hospital, we focused on 13 immune-related differential expression proteins and their related genes. An immune-related prognostic signature (IRPS) was constructed according to Cancer Genome Atlas (TCGA) dataset. The IRPS was verified in ArrayExpress (E-MTAB-4321) cohort and Shanghai General Hospital (General) cohort, separately. A total of 1010 BCa patients were involved in the study, including 405 BCa patients in TCGA cohort, 476 BCa patients in E-MTAB-4321 cohort and 129 MIBC patients in General cohort.

Result: It can be indicated that high IRPS score was related to poor 5-year overall survival and disease-free survival. The IRPS score was also evaluated its immune infiltration. We found that the IRPS score was adversely associated with GZMB, IFN-γ, PD-1, PD-L1. Additionally, higher IRPS score was significantly associated with more M2 macrophage and resting mast cell infiltration.

Conclusion: The study revealed a novel BCa prognostic signature based on IRPS score, which may be useful for BCa immunotherapies.
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http://dx.doi.org/10.1186/s12885-022-09783-yDOI Listing
June 2022

Sodium channel 1 subunit alpha SCNN1A exerts oncogenic function in pancreatic cancer via accelerating cellular growth and metastasis.

Arch Biochem Biophys 2022 Jun 14:109323. Epub 2022 Jun 14.

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China. Electronic address:

The identification of new diagnostic and therapeutic biomarkers might be helpful to understand molecular mechanism of cancer pathogenesis and develop anti-cancer targets. This study reported the alteration of Sodium channel 1 subunit alpha (SCNN1A) expression, its prognostic significance and biological roles in pancreatic cancer. Bioinformatics database was searched to explore the expression of SCNN1A in pancreatic cancer specimens and analysis results were further validated by qRT-PCR and Western blot assay. The correlation between SCNN1A expression and clinicopathological characteristics and its impact on survival outcome of pancreatic cancer patients were investigated using GEPIA database and Kaplan-Meier plotter. Loss- and gain-of-functional experiments in vitro were done to investigate the biological function of SCNN1A in pancreatic cancer. Bioinformatics analysis and validation experiment showed that SCNN1A was frequently overexpressed in pancreatic cancer specimens and cell lines (P < 0.001), and there were significant relevance between high SCNN1A expression and TP53 mutation (P < 0.05) as well as unfavorable prognosis of pancreatic cancer patients (HR for overall survival: 1.9, P = 0.003 and HR for disease-free survival: 1.7, P = 0.014). The silencing of SCNN1A suppressed cell proliferation, migration and invasion and induced cell apoptosis (P < 0.05), while its overexpression promoted aggressive phenotypes of pancreatic cancer cells in vitro (P < 0.05). SCNN1A possessed oncogenic function and its dysregulation could be implicated in the development and metastasis of pancreatic cancer.
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http://dx.doi.org/10.1016/j.abb.2022.109323DOI Listing
June 2022

Scalp EEG functional connection and brain network in infants with West syndrome.

Neural Netw 2022 Jun 7;153:76-86. Epub 2022 Jun 7.

Department of Neurology, the Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzho, 310003, China. Electronic address:

The common age-dependent West syndrome can be diagnosed accurately by electroencephalogram (EEG), but its pathogenesis and evolution remain unclear. Existing research mainly aims at the study of West seizure markers in time/frequency domain, while less literature uses a graph-theoretic approach to analyze changes among different brain regions. In this paper, the scalp EEG based functional connectivity (including Correlation, Coherence, Time Frequency Cross Mutual Information, Phase-Locking Value, Phase Lag Index, Weighted Phase Lag Index) and network topology parameters (including Clustering coefficient, Feature path length, Global efficiency, and Local efficiency) are comprehensively studied for the prognostic analysis of the West episode cycle. The scalp EEGs of 15 children with clinically diagnosed string spasticity seizures are used for prospective study, where the signal is divided into pre-seizure, seizure, and post-seizure states in 5 typical brain wave rhythm frequency bands (δ (1-4 Hz), θ (4-8 Hz), α (8-13 Hz), β (13-30 Hz), and γ (30-80 Hz)) for functional connectivity analysis. The study shows that recurrent West seizures weaken connections between brain regions responsible for cognition and intelligence, while brain regions responsible for information synergy and visual reception have greater variability in connectivity during seizures. It is observed that the changes inβandγfrequency bands of the multiband brain network connectivity patterns calculated by Corr and WPLI can be preliminarily used as judgment of seizure cycle changes in West syndrome.
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http://dx.doi.org/10.1016/j.neunet.2022.05.029DOI Listing
June 2022

Acute stress deteriorates breast meat quality of Ross 308 broiler chickens by inducing redox imbalance and mitochondrial dysfunction.

J Anim Sci 2022 Jun 17. Epub 2022 Jun 17.

College of Animal Science and Technology; Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.

This study investigated the effects of acute stress on breast meat quality, redox status and mitochondrial function in pectoralis major (PM) muscle of broilers. A total of 168 broiler chickens (42-day-old, Ross 308) were randomly divided into control (CON) and pre-slaughter transport (T) treatments. A broiler was an experimental unit. Each treatment consisted of 84 broilers, and they were put in 12 crates with 7 broilers each. Broilers in the T group were transported according to a designed protocol, and the CON broilers were kept in crates under normal living conditions before slaughtering. Based on the meat quality traits assessed at postmortem 24 h, all PM muscles of the transported broilers were further classified into normal (T-NOR) and pale, soft and exudative (PSE)-like (T-PSE) groups for the determination of redox status in PM muscle and isolated mitochondria, energy metabolites, mitochondrial electron transport chain complexes activities, as well as mitochondrial function-modulating genes expression. Compared with CON, the extent of lipid peroxidation as well as protein oxidation were significantly increased in both PM muscles and mitochondria in T-PSE (P < 0.05), whereas not in T-NOR. Higher activities of glutathione peroxidase, total superoxide dismutase and Cu-Zn superoxide dismutase were observed in PM muscle of T-NOR broilers as compared with CON (P < 0.05). Pre-slaughter transport increased the generation of reactive oxygen species, as well as enhanced antioxidant capacity in PM mitochondria of broilers (P < 0.05). Compared with CON, the ATP content, activities of complex I and III, as well as relative mitochondrial membrane potential and swelling were significantly decreased in T-PSE (P < 0.05), whereas no significant changes in either ATP content or complex I activity were observed in T-NOR. Pre-slaughter transport enhanced the mRNA expression of regulators involved in the glutathione system, thioredoxin 2 system and mitochondrial biosynthesis in PM muscle of broilers (P < 0.05). Moreover, we noticed a more evident enhancement effect in T-NOR than in T-PSE (P < 0.05). Overall, this work indicates that acute stress-induced redox imbalance and mitochondrial dysfunction have significant implications for the development of PSE-like meat.
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http://dx.doi.org/10.1093/jas/skac221DOI Listing
June 2022

Distinguishing Tumor Admixed in a Radiation Necrosis (RN) Background: H and H MR With a Novel Mouse Brain-Tumor/RN Model.

Front Oncol 2022 30;12:885480. Epub 2022 May 30.

Department of Radiology, Washington University, Saint Louis, MO, United States.

Purpose: Distinguishing radiation necrosis (RN) from recurrent tumor remains a vexing clinical problem with important health-care consequences for neuro-oncology patients. Here, mouse models of pure tumor, pure RN, and admixed RN/tumor are employed to evaluate hydrogen (H) and deuterium (H) magnetic resonance methods for distinguishing RN vs. tumor. Furthermore, proof-of-principle, range-finding deuterium (H) metabolic magnetic resonance is employed to assess glycolytic signatures distinguishing RN vs. tumor.

Materials And Methods: A pipeline of common quantitative H MRI contrasts, including an improved magnetization transfer ratio (MTR) sequence, and H magnetic resonance spectroscopy (MRS) following administration of H-labeled glucose, was applied to C57BL/6 mouse models of the following: (i) late time-to-onset RN, occurring 4-5 weeks post focal 50-Gy (50% isodose) Gamma Knife irradiation to the left cerebral hemisphere, (ii) glioblastoma, growing ~18-24 days post implantation of 50,000 mouse GL261 tumor cells into the left cerebral hemisphere, and (iii) mixed model, with GL261 tumor growing within a region of radiation necrosis (H MRI only). Control C57BL/6 mice were also examined by H metabolic magnetic resonance.

Results: Differences in quantitative H MRI parametric values of R1, R2, ADC, and MTR comparing pure tumor vs. pure RN were all highly statistically significant. Differences in these parameter values and DCE for tumor vs. RN in the mixed model (tumor growing in an RN background) are also all significant, demonstrating that these contrasts-in particular, MTR-can effectively distinguish tumor vs. RN. Additionally, quantitative H MRS showed a highly statistically significant dominance of aerobic glycolysis (glucose ➔ lactate; fermentation, Warburg effect) in the tumor vs. oxidative respiration (glucose ➔ TCA cycle) in the RN and control brain.

Conclusions: These findings, employing a pipeline of quantitative H MRI contrasts and H MRS following administration of H-labeled glucose, suggest a pathway for substantially improving the discrimination of tumor vs. RN in the clinic.
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http://dx.doi.org/10.3389/fonc.2022.885480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196939PMC
May 2022

Accelerated aging of all-inorganic, interface-stabilized perovskite solar cells.

Science 2022 Jun 16:eabn5679. Epub 2022 Jun 16.

Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.

To understand degradation routes and improve the stability of perovskite solar cells (PSCs), accelerated aging tests are needed. Here, we use elevated temperatures (up to 110 Celsius) to quantify the accelerated degradation of encapsulated CsPbI PSCs under constant illumination. Incorporating a 2D CsPbICl capping layer between the perovskite active layer and hole-transport layer stabilizes the interface while increasing power conversion efficiency of the all-inorganic PSCs from 14.9% to 17.4%. Devices with this 2D capping layer did not degrade at 35 Celsius and required >2100 hours at 110 Celsius under constant illumination to degrade by 20% of their initial efficiency. Degradation acceleration factors based on the observed Arrhenius temperature dependence predict intrinsic lifetimes of 51,000 ± 7,000 hours (>5 years) operating continuously at 35 Celsius.
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http://dx.doi.org/10.1126/science.abn5679DOI Listing
June 2022

Molecular-docking-guided design, palladium-catalyzed synthesis and anticancer activity of paclitaxel-benzoxazoles hybrids.

Sci Rep 2022 Jun 15;12(1):10021. Epub 2022 Jun 15.

Department of Pharmacy, The First Afflicted Hospital of Chengdu Medical College, No. 278, Baoguang Rd, Xindu Region, Chengdu, 610500, People's Republic of China.

A series of new paclitaxel-benzoxazoles hybrids were designed based on both the molecular docking mode of beta-tubulin with paclitaxel derivatives (7a and 7g), and the activity-structure relationship of C-13 side chain in paclitaxel. Palladium-catalyzed direct Csp-H arylation of benzoxazoles with different aryl-bromides was used as the key synthetic strategy for the aryl-benzoxazoles moieties in the hybrids. Twenty-six newly synthesized hybrids were screened for their antiproliferative activity against human cancer cell lines such as human breast cancer cells (MDA-MB-231) and liver hepatocellular cells (HepG2) by the MTT assay and results were compared with paclitaxel. Interestingly, most hybrids (7a-7e, 7i, 7k, 7l, 7A, 7B, 7D and 7E) showed significantly active against both cell lines at concentration of 50 µM, which indicated that the hybrid strategy is effective to get structural simplified paclitaxel analogues with high anti-tumor activity.
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http://dx.doi.org/10.1038/s41598-022-14172-3DOI Listing
June 2022

The Effect of Alfentanil on Emergence Delirium Following General Anesthesia in Children: A Randomized Clinical Trial.

Paediatr Drugs 2022 Jul 13;24(4):413-421. Epub 2022 Jun 13.

Department of Anesthesiology, Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing, China.

Background: Emergence delirium can occur after general anesthesia in children. An intravenous infusion of alfentanil may reduce the incidence or severity of emergence delirium after sevoflurane anesthesia.

Objective: The study aimed to investigate the effects of alfentanil intravenous infusion on emergence delirium and other perioperative complications.

Method: This was a single-center, randomized, placebo-controlled, double-blind clinical trial. A total of 172 children undergoing ambulatory dental treatment were randomized into three groups. Alfentanil group Alf2 received 0.2 μg/kg/min of alfentanil for continuous infusion, alfentanil group Alf4 received 0.4 μg/kg/min alfentanil, and the saline group (group Sal) received a continuous infusion of normal saline, with the same volume as the two other groups, as a placebo. The incidence of emergence delirium (assessed by the Paediatric Anaesthesia Emergence Delirium [PAED] scale), hemodynamic parameters, and recovery characteristics were recorded during the recovery period. The Aono scale was also used to assess for emergence delirium. A WeChat applet was designed to facilitate a caregiver teleconsultation and to provide feedback on postoperative nausea and vomiting and any other complications after discharge.

Results: The incidence of emergence delirium in group Alf2 (22.9%) and group Alf4 (21.1%) was significantly lower than that observed in the Sal group (48.3%). The PAED scores in group Alf2 (6.4 ± 3.5) and group Alf4 (5.8 ± 3.8) were significantly lower than those for group Sal (9.6 ± 5.1) (p < 0.01). Ten children in the Alf4 group needed manual ventilatory assistance to maintain end-tidal carbon dioxide (ETCO) < 55 mm; children in group Alf2 did not. There was no significant difference between the discharge time of groups Alf2 and Sal (31.2 ± 4.64 vs 30.5 ± 2.82 min; 0.659 [95% confidence interval {CI} -1.052 to 2.369], p = 0.643); the time to discharge of group Alf4 (35.16 ± 3.97 min) was significantly longer than that of groups Alf2 and Sal (p < 0.01). The incidence of nausea and vomiting was similar in the three groups. No other clinically relevant adverse events were observed.

Conclusions: Intravenous infusion of 0.2 μg/kg/min and 0.4 μg/kg/min alfentanil decreased the incidence of emergence delirium in the post-anesthesia care unit. The 0.2 μg/kg/min dose of alfentanil resulted in less respiratory depression and discharge delay than the 0.4 μg/kg/min alfentanil dose.

Trial Registration: Chinese Clinical Trial Registry (ChiCTR2100043320).
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http://dx.doi.org/10.1007/s40272-022-00510-5DOI Listing
July 2022

A long-acting interleukin-7, rhIL-7-hyFc, enhances CAR T cell expansion, persistence, and anti-tumor activity.

Nat Commun 2022 Jun 13;13(1):3296. Epub 2022 Jun 13.

Division of Oncology, Department of Medicine, Washington University in St. Louis, St. Louis, MO, USA.

Chimeric antigen receptor (CAR) T cell therapy is routinely used to treat patients with refractory hematologic malignancies. However, a significant proportion of patients experience suboptimal CAR T cell cytotoxicity and persistence that can permit tumor cell escape and disease relapse. Here we show that a prototype pro-lymphoid growth factor is able to enhance CAR T cell efficacy. We demonstrate that a long-acting form of recombinant human interleukin-7 (IL-7) fused with hybrid Fc (rhIL-7-hyFc) promotes proliferation, persistence and cytotoxicity of human CAR T cells in xenogeneic mouse models, and murine CAR T cells in syngeneic mouse models, resulting in long-term tumor-free survival. Thus, rhIL-7-hyFc represents a tunable clinic-ready adjuvant for improving suboptimal CAR T cell activity.
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http://dx.doi.org/10.1038/s41467-022-30860-0DOI Listing
June 2022

An Exploratory Study of Neoadjuvant Cetuximab Followed by Cetuximab and Chemoradiotherapy in Women With Newly Diagnosed Locally Advanced Cervical Cancer.

Am J Clin Oncol 2022 07 7;45(7):286-293. Epub 2022 Jun 7.

Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine.

Objectives: This study explored the feasibility of cetuximab with chemoradiation in women with cervical carcinoma and evaluated fluorine-18 fluorodeoxyglucose-positron emission tomography/computed tomography (18F-FDG-PET/CT) to assess early response to cetuximab (NCT00292955).

Patients And Methods: Eligible patients with International Federation of Gynecology and Obstetrics (FIGO) stage IB-IVB invasive carcinoma of the uterine cervix were treated on 1 of 3 dose levels (DL). DL1 consisted of neoadjuvant cetuximab, then concurrent radiotherapy with cetuximab 250 mg/m2/cisplatin 40 mg/m2, followed by weekly cetuximab. DL2 consisted of radiotherapy with cetuximab 200 mg/m2 and cisplatin 30 mg/m2. DL3 consisted of radiotherapy with cetuximab 250 mg/m2 and cisplatin 30 mg/m2. Patients underwent 18F-FDG-PET/CT before treatment, after neoadjuvant cetuximab, and at the end of treatment.

Results: Of the 21 patients enrolled, 9, 3, and 9 were treated in DL1, DL2, and DL3, respectively. DL1 required dose reductions due to gastrointestinal toxicities. DL2 and 3 were tolerated with 1 dose-limiting toxicity (grade 4 renal failure) at DL3. Following 3 weekly treatments of neoadjuvant cetuximab in DL1, 7 patients had maximum standardized uptake value changes on 18F-FDG-PET/CT consistent with response to cetuximab. Of the 12 patients with locally advanced disease, eleven evaluable patients had no evidence of disease on 18F-FDG-PET/CT at treatment end. Five-year progression-free survival and overall survival rates for all patients were 57.5% and 58.5%, respectively.

Conclusions: Cetuximab with cisplatin 30 mg/m2 and radiotherapy was tolerated. 18F-FDG-PET/CT demonstrated early evidence of response to neoadjuvant cetuximab. With advances in precision oncology and the recent approval of pembrolizumab in metastatic cervical cancer, dual-target inhibition with an epidermal growth factor receptor inhibitor may be a promising treatment in the future.
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http://dx.doi.org/10.1097/COC.0000000000000926DOI Listing
July 2022

Activation and blockade of 5-HT receptors in the dorsal hippocampus enhance working and hippocampus-dependent memories in the unilateral 6-hydroxydopamine lesioned rats.

Behav Brain Res 2022 Jun 8;431:113952. Epub 2022 Jun 8.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. Electronic address:

Cognitive dysfunction is a common symptom in Parkinson's disease (PD). Serotonin4 (5-HT) receptors are richly expressed in the dorsal hippocampus (dHIPP) and play an important role in cognitive activities. However, the mechanism underlying the role of dHIPP 5-HT receptors in PD-related cognitive dysfunction remains unclear. Here we found that unilateral 6-hydroxydopamine lesions of the medial forebrain bundle increased the protein expression of 5-HT receptors in the dHIPP, decreased hippocampal theta rhythm, and impaired working memory and hippocampus-dependent memory in the T-maze and hole-board test, respectively. Both activation and blockade of dHIPP 5-HT receptors (agonist BIMU8 and antagonist GR113808) improved working memory and hippocampus-dependent memory in the lesioned rats, but not in sham rats. Activation of dHIPP 5-HT receptors increased hippocampal theta rhythm in the lesioned rats. The neurochemical studies showed that injection of BIMU8, GR113808 or GR113808/BIMU8 in the dHIPP increased the levels of dopamine in the medial prefrontal cortex (mPFC), dHIPP and amygdala, and the level of 5-HT in the amygdala in the lesioned rats, but not in sham rats. Injection of GR113808 or GR113808/BIMU8 into the dHIPP also increased the levels of noradrenaline in the mPFC, dHIPP and amygdala only in the lesioned rats. These results suggest that activation or blockade of dHIPP 5-HT receptors may improve the cognitive impairments in parkinsonian rats, which may be due to the increase of hippocampal theta rhythm, up-regulated expressions of 5-HT receptors in the dHIPP and the changes in the levels of monoamines in the relative brain areas.
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http://dx.doi.org/10.1016/j.bbr.2022.113952DOI Listing
June 2022

Design of Sports Training Simulation System for Children Based on Improved Deep Neural Network.

Comput Intell Neurosci 2022 31;2022:9727415. Epub 2022 May 31.

Jiaxing College Sports and Military Training Department, Jiaxing University, Jiaxing, Zhejiang 314000, China.

With the development of AI technology, human-computer interaction technology is no longer the traditional mouse and keyboard interaction. AI and VR have been widely used in early childhood education. In the process of the slow development and application of voice interaction, visual interaction, action interaction, and other technologies, multimodal interaction technology system has become a research hotspot. In this paper, dynamic image capture and recognition technology is integrated into early childhood physical education for intelligent interaction. According to the basic movement process and final node matching in children's sports training to judge children's physical behavior ability, attention is paid to identify the accuracy and safety of movement. The input images and questions are from the abstract clipart dataset of dynamic image recognition and the self-made 3D dataset of Web3D dynamic motion scene with the same style, which is similar to the action content in the actual preschool training teaching. Therefore, according to the idea of process capture and target recognition, on the basis of the original conditions of the recognition model, a new recognition model is developed through Zheng's target detector. The modified model is characterized by higher accuracy. Weapons need to combine process recognition and result recognition. The experimental results show that the improved model has the obvious advantages of high precision and fast speed, which provides a new research idea for the development of children's physical training simulation.
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http://dx.doi.org/10.1155/2022/9727415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9173887PMC
June 2022

Model Predictive Control of a Novel Wheeled-Legged Planetary Rover for Trajectory Tracking.

Sensors (Basel) 2022 May 30;22(11). Epub 2022 May 30.

State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Amid increasing demands for planetary exploration, wide-range autonomous exploration is still a great challenge for existing planetary rovers, which calls for new planetary rovers with novel locomotive mechanisms and corresponding control strategies. This paper proposes a novel wheeled-legged mechanism for the design of planetary rovers. The leg suspension utilizes a rigid-flexible coupling mechanism with a hybrid serial-parallel topology. First, the kinematic model is derived. Then, a control strategy for the wheeled-legged rover that includes a trajectory tracking module based on the model predictive control, the steering strategy, and the wheel speed allocation algorithm is proposed. After that, three groups of cosimulations with different trajectories and speeds, and experiments are carried out. Results of both the simulations and experiments validate the proposed control method.
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http://dx.doi.org/10.3390/s22114164DOI Listing
May 2022

Genome-Wide Identification and Analysis of Ariadne Gene Family Reveal Its Genetic Effects on Agronomic Traits of .

Int J Mol Sci 2022 Jun 3;23(11). Epub 2022 Jun 3.

Key Laboratory of Biology and Genetics Improvement of Oil Crops, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.

E3 ligases promote protein ubiquitination and degradation, which regulate every aspect of eukaryotic life. The Ariadne (ARI) proteins of RBR (ring between ring fingers) protein subfamily has been discovered as a group of potential E3 ubiquitin ligases. Only a few available research studies show their role in plant adaptations processes against the external environment. Presently, the functions of ARI proteins are largely unknown in plants. Therefore, in this study, we performed genome-wide analysis to identify the gene family and explore their potential importance in . A total of 39 genes were identified in the genome and were classified into three subfamilies (A, B and C) based on phylogenetic analysis. The protein-protein interaction networks and enrichment analysis indicated that genes could be involved in endoreduplication, DNA repair, proteasome assembly, ubiquitination, protein kinase activity and stress adaptation. The transcriptome data analysis in various tissues provided us an indication of some genes' functional importance in tissue development. We also identified potential genes that were significantly responsive towards the abiotic stresses. Furthermore, eight genes were identified as candidate genes for multiple agronomic traits through association mapping analysis in ; among them, , which is the ortholog of , was significantly associated with ten agronomic traits. This study provided useful information on genes, which could aid targeted functional research and genetic improvement for breeding in .
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http://dx.doi.org/10.3390/ijms23116265DOI Listing
June 2022

Potential Antiviral Strategy Exploiting Dependence of SARS-CoV-2 Replication on Lysosome-Based Pathway.

Int J Mol Sci 2022 May 31;23(11). Epub 2022 May 31.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130022, China.

The recent novel coronavirus (SARS-CoV-2) disease (COVID-19) outbreak created a severe public health burden worldwide. Unfortunately, the SARS-CoV-2 variant is still spreading at an unprecedented speed in many countries and regions. There is still a lack of effective treatment for moderate and severe COVID-19 patients, due to a lack of understanding of the SARS-CoV-2 life cycle. Lysosomes, which act as "garbage disposals" for nearly all types of eukaryotic cells, were shown in numerous studies to support SARS-CoV-2 replication. Lysosome-associated pathways are required for virus entry and exit during replication. In this review, we summarize experimental evidence demonstrating a correlation between lysosomal function and SARS-CoV-2 replication, and the development of lysosomal perturbation drugs as anti-SARS-CoV-2 agents.
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http://dx.doi.org/10.3390/ijms23116188DOI Listing
May 2022

Activation of CD44 signaling in leader cells induced by tumor-associated macrophages drives collective detachment in luminal breast carcinomas.

Cell Death Dis 2022 Jun 9;13(6):540. Epub 2022 Jun 9.

Department of Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Collective detachment of cancer cells at the invading front could generate efficient metastatic spread. However, how cancer cell clusters shed from the leading front remains unknown. We previously reported that the dynamic expression of CD44 in breast cancers (BrCas) at collectively invading edges was associated with tumor-associated macrophages (TAMs). In this study, we first observed that the highly expressed CD44 (CD44) cancer cell clusters were located in the BrCa circulating vessels, accompanied by CD206 TAMs. Next, we identified that the cancer cell clusters can be converted to an invasive CD44 state which was induced by TAMs, thus giving rise to CD44-associated signaling mediated cohesive detachment. Then, we showed that disrupting CD44-signaling inhibited the TAMs triggered cohesive detaching using 3D organotypic culture and mouse models. Furthermore, our mechanistic study showed that the acquisition of CD44 state was mediated by the MDM2/p53 pathway activation which was induced by CCL8 released from TAMs. Blocking of CCL8 could inhibit the signaling cascade which decreased the CD44-mediated cohesive detachment and spread. Our findings uncover a novel mechanism underlying collective metastasis in BrCas that may be helpful to seek for potential targets.
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http://dx.doi.org/10.1038/s41419-022-04986-4DOI Listing
June 2022

Enzyme-free nucleic acid dual-amplification strategy combined with mimic enzyme catalytic precipitation reaction for the photoelectrochemical detection of microRNA-21.

Mikrochim Acta 2022 06 10;189(7):249. Epub 2022 Jun 10.

Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.

A novel photoelectrochemical (PEC) biosensor based on an enzyme-free nucleic acid dual-amplification strategy combined with a mimic enzyme to catalyze the deposition of a quencher is reported for the ultrasensitive detection of miRNA-21. A limited amount of target miRNA-21 can trigger the formation of long DNA duplexes on the electrode, owing to the synergistic effect of the enzyme-free nucleic acid dual-amplification strategy of entropy-driven strand displacement reaction (ESDR) amplification and hybridization chain reaction (HCR) amplification. The embedded manganese porphyrin (MnPP) in the long DNA duplexes acts as a horseradish peroxidase (HRP)-mimicking enzyme to catalyze the transformation of benzo-4-chlorohexadienone on the electrode surface, resulting in a significant reduction in photocurrent intensity. As a photosensitive material, BiOCl-BiOI is used as a tag to provide strong initial PEC signals. Based on the cascade integration of the enzyme-free nucleic acid dual-amplification strategy and the mimic enzyme-catalyzed precipitation reaction, the current PEC biosensor exhibits outstanding performance for miRNA-21 detection with an ultralow detection limit (33 aM) and a wide quantification range (from 100 aM to 1 nM). This work provides a new avenue toward the ultrasensitive detection of miRNAs, and is expected to be used for clinical and biochemical samples. A unique PEC biosensor with the BiOCl-BiOI composite, as the photosensitive material, has been developed for ultrasensitive miRNA-21 determination based on the combination of an enzyme-free nucleic acid dual-amplification strategy and mimic enzyme catalytic precipitation reaction.
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http://dx.doi.org/10.1007/s00604-022-05345-yDOI Listing
June 2022
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