Publications by authors named "Feng Cao"

431 Publications

Extracellular vesicles and pancreatitis: mechanisms, status and perspectives.

Int J Biol Sci 2021 11;17(2):549-561. Epub 2021 Jan 11.

Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Comprehensive reviews and large population-based cohort studies have played an important role in the diagnosis and treatment of pancreatitis and its sequelae. The incidence and mortality of pancreatitis have been reduced significantly due to substantial advancements in the pathophysiological mechanisms and clinically effective treatments. The study of extracellular vesicles (EVs) has the potential to identify cell-to-cell communication in diseases such as pancreatitis. Exosomes are a subset of EVs with an average diameter of 50~150 nm. Their diverse and unique constituents include nucleic acids, proteins, and lipids, which can be transferred to trigger phenotypic changes of recipient cells. In recent years, many reports have indicated the role of EVs in pancreatitis, including acute pancreatitis, chronic pancreatitis and autoimmune pancreatitis, suggesting their potential influence on the development and progression of pancreatitis. Plasma exosomes of acute pancreatitis can effectively reach the alveolar cavity and activate alveolar macrophages to cause acute lung injury. Furthermore, upregulated exosomal miRNAs can be used as biomarkers for acute pancreatitis. Here, we summarized the current understanding of EVs in pancreatitis with an emphasis on their biological roles and their potential use as diagnostic biomarkers and therapeutic agents for this disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.54858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893579PMC
January 2021

TMEM176A acts as a tumor suppressor gene in pancreatic cancer by inhibiting ERK signaling.

Discov Med 2020 Nov-Dec;30(161):145-153

Department of General Surgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.

Background: Pancreatic cancer is the 7th leading cause of cancer-related death worldwide. Aberrant expressions of transmembrane 176A (TMEM176A) were found in multiple cancer types. However, the expression and function of TMEM176A remain unclear in pancreatic cancer.

Materials And Methods: Immunohistochemistry, flow cytometry, western blot, and transwell were applied on investigating samples from pancreatic cancer patients and pancreatic cancer cell lines.

Results: Analysis based on the TCGA database showed that a high level of TMEM176A was associated with a better relapse-free survival rate (P = 0.012). Further, the results of tissue microarray showed patients with a high level of TMEM176A were associated with lymph node metastasis (P = 0.045) and a better overall survival rate (P = 0.032). Overexpression of TMEM176A in Capan-1 and PANC-1 cells suppressed cell proliferation, cell invasion, and migration, and induced apoptosis in pancreatic cancer cells. TMEM176A suppressed ERK signaling in pancreatic cancer.

Conclusion: TMEM176A suppresses the growth and migration of pancreatic cancer cells by inhibiting ERK signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
February 2021

Latanoprost-loaded cyclodextrin microaggregate suspension eye drops for enhanced bioavailability and stability.

Eur J Pharm Sci 2021 Feb 12;160:105758. Epub 2021 Feb 12.

Department of Pharmaceutical, School of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing 210009, China. Electronic address:

Objective: The bioavailability of conventional eye drops is very low due to different physiological barriers. Commercial latanoprost (LAT) eye drops (Xalatan®) need to be refrigerated and protected from light. The purpose of this study was to develop novel LAT eye drops to improve ocular bioavailability and stability.

Methods: Ophthalmic suspension containing LAT/γ-cyclodextrin (γCD) aggregates was designed and the preparation process was sufficiently studied. The prepared formulations were evaluated for pH, viscosity, osmolality, particle size, entrapment efficiency and in vitro release study. In vitro permeability study using Human Corneal Epithelial Cells and in vivo studies on rabbits were also performed.

Results: LAT/γCD aggregates were formed and confirmed by scanning electron microscopy. LAT/γCD eye drops showed obvious sustained release profiles and were more stable than Xalatan®. In vitro corneal permeation study indicated LAT/γCD eye drops had no significant cytotoxicity and had higher cell permeability. In vivo precorneal retention study showed AUC , C, and mean residence time (MRT) of LAT/γCD eye drops were 3.98, 2.12, and 2.07 times higher than those of Xalatan®, respectively. In vivo ocular distribution study revealed that AUC , C, and MRT for latanoprost acid in aqueous humor exhibited 2.60-fold, 1.36-fold, and 1.99-fold increase in LAT/γCD eye drops group than those of Xalatan® group, respectively.

Conclusion: Cyclodextrin microaggregate suspension eye drops represent a potential strategy for enhanced bioavailability and stability of LAT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejps.2021.105758DOI Listing
February 2021

Treatment of Complex Anal Fistula by Video-Assisted Anal Fistula Treatment Combined with Anal Fistula Plug: A Single-Center Study.

Surg Innov 2021 Feb 10:1553350621992924. Epub 2021 Feb 10.

Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, People's Republic of China.

. The surgical treatment of complex anal fistulae is very challenging because of the incidence of incontinence after traditional approaches. There are no studies on the role of video-assisted anal fistula treatment (VAAFT) combined with anal fistula plug (AFP) in the complex anal fistulae. The aim of this study was to demonstrate the efficacy of treating complex anal fistulae using VAAFT combined with AFP. . This was a retrospective, nonrandomized observational study. 57 consecutive patients with complex anal fistulae who had undergone the VAAFT with AFP in our hospital between April 2016 and December 2019 were included. The primary outcomes were the cure rate, recurrence rate, and Wexner incontinence scores; the secondary outcomes were surgery time, blood loss, wound healing time postoperatively, pain, and patient satisfaction. . All 57 patients completed the surgery and follow-up, with an average follow-up time of 28 months; 6 patients suffered with recurrence (recurrence rate: 10.5%). The average surgery time was 57.9 minutes, and the average wound healing time was 46 days. There were no severe postoperative complications, and anal sphincter function was protected in all patients. . The treatment of complex anal fistula by VAAFT combined with AFP is safe and effective, has a high healing rate and few postoperative complications, and is a promising surgery that can effectively protect the patient's anal sphincter function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1553350621992924DOI Listing
February 2021

A narrative review of acute pancreatitis and its diagnosis, pathogenetic mechanism, and management.

Ann Transl Med 2021 Jan;9(1):69

Department of General Surgery, Xuan Wu Hospital, Capital Medical University, Beijing, China.

Acute pancreatitis (AP) is an inflammatory disease that can progress to severe acute pancreatitis (SAP), which increases the risk of death. AP is characterized by inappropriate activation of trypsinogen, infiltration of inflammatory cells, and destruction of secretory cells. Other contributing factors may include calcium (Ca) overload, mitochondrial dysfunction, impaired autophagy, and endoplasmic reticulum (ER) stress. In addition, exosomes are also associated with pathophysiological processes of many human diseases and may play a biological role in AP. However, the pathogenic mechanism has not been fully elucidated and needs to be further explored to inform treatment. Recently, the treatment guidelines have changed; minimally invasive therapy is advocated more as the core multidisciplinary participation and "step-up" approach. The surgical procedures have gradually changed from open surgery to minimally invasive surgery that primarily includes percutaneous catheter drainage (PCD), endoscopy, small incision surgery, and video-assisted surgery. The current guidelines for the management of AP have been updated and revised in many aspects. The type of fluid to be used, the timing, volume, and speed of administration for fluid resuscitation has been controversial. In addition, the timing and role of nutritional support and prophylactic antibiotic therapy, as well as the timing of the surgical or endoscopic intervention, and the management of complications still have many uncertainties that could negatively impact the prognosis and patients' quality of life. Consequently, to inform clinicians about optimal treatment, we aimed to review recent advances in the understanding of the pathogenesis of AP and its diagnosis and management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-4802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859757PMC
January 2021

Vacancy ordering induced topological electronic transition in bulk EuZnSb.

Sci Adv 2021 Feb 5;7(6). Epub 2021 Feb 5.

School of Materials Science and Engineering and Institute of Materials Genome and Big Data, Harbin Institute of Technology, Shenzhen 518055, P.R. China.

Metal-semiconductor transitions from changes in edge chirality from zigzag to armchair were observed in many nanoribbon materials, especially those based on honeycomb lattices. Here, this is generalized to bulk complex Zintl semiconductors, exemplified by EuZnSb where the Zn vacancy ordering plays an essential role. Five EuZnSb structural models are proposed to guide transmission electron microscopy imaging. Zigzag vacancy ordering models show clear metallicity, while the armchair models are semiconducting with indirect bandgaps that monotonously increase with the relative distances between neighboring ZnSb chains. Topological electronic structure changes based on cation ordering in a Zintl compound point toward tunable and possibly switchable topological behavior, since cations in these are often mobile. Thus, their orderings can often be adjusted by temperature, minor alloying, and other approaches. We explain the electronic structure of an interesting thermoelectric and point the way to previously unidentified types of topological electronic transitions in Zintl compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abd6162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864570PMC
February 2021

Gait Optimization Method for Humanoid Robots Based on Parallel Comprehensive Learning Particle Swarm Optimizer Algorithm.

Front Neurorobot 2020 15;14:600885. Epub 2021 Jan 15.

School of Electronic and Information Engineering, Suzhou University of Science and Technology, Suzhou, China.

To improve the fast and stable walking ability of a humanoid robot, this paper proposes a gait optimization method based on a parallel comprehensive learning particle swarm optimizer (PCLPSO). Firstly, the key parameters affecting the walking gait of the humanoid robot are selected based on the natural zero-moment point trajectory planning method. Secondly, by changing the slave group structure of the PCLPSO algorithm, the gait training task is decomposed, and a parallel distributed multi-robot gait training environment based on RoboCup3D is built to automatically optimize the speed and stability of bipedal robot walking. Finally, a layered learning approach is used to optimize the turning ability of the humanoid robot. The experimental results show that the PCLPSO algorithm achieves a quickly optimal solution, and the humanoid robot optimized possesses a fast and steady gait and flexible steering ability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnbot.2020.600885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843375PMC
January 2021

Red to Near-Infrared Mechanochromism from Metal-free Polycrystals: Noncovalent Conformational Locks Facilitating Wide-Range Redshift.

Angew Chem Int Ed Engl 2021 Jan 27. Epub 2021 Jan 27.

Huzhou University, Department of Material Chemistry, CHINA.

Piezochromic organic materials that present a large difference in fluorescence wavelength in the near-infrared region have important potential applications; however, few such metal-free luminophores have been reported. In this study, we design and prepare π-conjugated electron acceptors whose planar conformation can be locked by the hydrogen-bonded interactions. The formation of a planar fused-ring geometry via noncovalent conformational locking can narrow the optical band gap, enhance the molecular stability and rigidity, as well as increase the radiative rate. As expected, the polymorphs Re-phase and Ni-phase emit the high-brightness fluorescence with wavelength maxima (λem,max) at 615 and 727 nm, respectively. Upon full grinding, the λem,max of Re-phase is bathochromically shifted to 775 nm. Detailed spectrophotometric and single-crystal X-ray analyses demonstrate that the ground powder of Re-phase becomes metastable as a consequence of noncovalent conformational locking and that the red to near-infrared (large colour difference) mechanochromism arises from the high degree of conformational coplanarity. This strategy is both conceptually and synthetically simple and offers a promising approach to the development of organic piezochromic materials with wide-range redshift and excellent penetrability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202100301DOI Listing
January 2021

Identification of -benzothiazolyl-2-benzenesulfonamides as novel ABCA1 expression upregulators.

RSC Med Chem 2020 Mar 11;11(3):411-418. Epub 2020 Mar 11.

Hebei Key Laboratory of Organic Functional Molecules , College of Chemistry and Materials Science , Hebei Normal University , Shijiazhuang , 050024 , China . Email:

ATP binding cassette transporter A1 (ABCA1) is a critical transporter that mediates cellular cholesterol efflux from macrophages to apolipoprotein A-I (ApoA-I). Therefore, increasing the expression level of ABCA1 is anti-atherogenic and ABCA1 expression upregulators have become novel choices for atherosclerosis treatment. In this study, a series of -benzothiazolyl-2-benzenesulfonamides, based on the structure of WY06 discovered in our laboratory, were designed and synthesized as novel ABCA1 expression upregulators. Based on an ABCA1 upregulatory cell model, ABCA1 upregulation of target compounds was evaluated. Compounds , , and have good upregulated ABCA1 expression activities, with EC values of 0.97, 0.37, and 0.41 μM, respectively. A preliminary structure-activity relationship is summarized. Replacing the methoxy group on the benzothiazole moiety of WY06 with a fluorine or chlorine atom and exchanging the ester group with a cyano group resulted in more potent ABCA1 upregulating activity. Moreover, compound increased ABCA1 mRNA and protein expression and significantly promoted cholesterol efflux in RAW264.7 cells. In conclusion, -benzothiazolyl-2-benzenesulfonamides were identified as novel ABCA1 expression upregulators.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9md00556kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593777PMC
March 2020

Bioinformatics Analysis of Differentially Expressed Genes and Protein-Protein Interaction Networks Associated with Functional Pathways in Ulcerative Colitis.

Med Sci Monit 2021 Jan 19;27:e927917. Epub 2021 Jan 19.

Department of General Surgery, The Second Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).

BACKGROUND This bioinformatics study aimed to identify differentially expressed genes (DEGs) and protein-protein interaction (PPI) networks associated with functional pathways in ulcerative colitis based on 3 Gene Expression Omnibus (GEO) datasets. MATERIAL AND METHODS The GSE87466, GSE75214, and GSE48958 MINiML formatted family files were downloaded from the GEO database. DEGs were identified from the 3 datasets, and volcano maps and heat maps were drawn after R language standardization and analysis, respectively. Venn diagram software was used to identify common DEGs. PPI analysis of common DEGs was performed using the Search Tool for the Retrieval of Interacting Genes. Gene modules and hub genes were visualized in the PPI network using Cytoscape. Enrichment analysis was performed for all common DEGs, module genes, and hub genes. RESULTS A total of 90 DEGs were selected, which included 3 functional modules and 1 hub gene module. CXCL8 module genes were mainly enriched in cytokine-mediated signaling pathways and interleukin (IL)-10 signaling. CCL20 module genes were mainly enriched in the IL-17 signaling pathway and cellular response to IL-1. Hub gene modules mainly involved IL-10, IL-4, and IL-13 signaling pathways. CXCL8, CXCL1, and IL-1ß were the top 3 hub genes and were mainly involved in IL-10 signaling. CONCLUSIONS Bioinformatics analysis using 3 GEO datasets identified CXCL8, CXCL1, and IL-1ß, which are involved in IL-10 signaling, as the top 3 hub genes in ulcerative colitis. The findings from this study remain to be validated, but they may contribute to the further understanding of the pathogenesis of ulcerative colitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.927917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824989PMC
January 2021

Video-assisted anal fistula treatment combined with anal fistula plug for treatment of horseshoe anal fistula.

J Int Med Res 2021 Jan;49(1):300060520980525

Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objective: Horseshoe anal fistula is a common anorectal disease, and there is no standard procedure for its treatment. In this study, we performed a modified surgical procedure for the treatment of horseshoe anal fistula and investigated its efficacy and adverse effects.

Methods: We retrospectively analyzed the outcomes of video-assisted anal fistula treatment combined with an anal fistula plug (VAAFT-Plug) in 26 patients with a horseshoe anal fistula. The follow-up period ranged from 6 to 18 months. Preoperative and postoperative data were collected to analyze the cure rate, anal sphincter function, and incidence of complications.

Results: The surgeries were successfully performed in all patients, 23 of whom were cured (effective cure rate of 88.46%). Three patients developed recurrence and were cured after traditional surgery. No patients developed severe complications or postoperative anal incontinence. The VAAFT-Plug protocol was performed with a small incision in the fistula that subsequently promoted fistula healing and preserved sphincter function.

Conclusion: Although randomized controlled trials will be needed to fully validate these findings, our results suggest that VAAFT-Plug represents a promising treatment strategy for horseshoe anal fistulas. This technique preserves normal anal function and achieves satisfactory outcomes in most patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0300060520980525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809318PMC
January 2021

Coronavirus diseases 2019 and kidney injury: a brief review based on current evidence.

Chin Med J (Engl) 2021 Jan 5;Publish Ahead of Print. Epub 2021 Jan 5.

Department of Geriatric Nephrology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, The General Hospital of People's Liberation Army, Beijing 100853, China Department of Geriatric Cardiovascular Disease, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, The General Hospital of People's Liberation Army, Beijing 100853, China The Second Medical Center & National Clinical Research Center for Geriatric Diseases, The General Hospital of People's Liberation Army, Beijing 100853, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001311DOI Listing
January 2021

A comparative study on the adsorption behavior of pesticides by pristine and aged microplastics from agricultural polyethylene soil films.

Ecotoxicol Environ Saf 2021 Feb 16;209:111781. Epub 2020 Dec 16.

Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Environment, Nanjing Normal University, Nanjing 210023, PR China. Electronic address:

Compared with pristine agricultural polyethylene (PE) soil films microplastics (MPs), aged agricultural polyethylene (APE) soil films MPs have a rougher surface, more cracks and have some oxygen-containing functional groups that makes them adsorb organic pollutants, such as pesticides more easily. This may be more harmful to human beings than marine MPs as the agricultural soil films are closer to our living environment. But few works focused on the adsorption of pesticides on pristine or aged agricultural polyethylene soil films MPs. In order to promote the risk assessment of co-exposure of pesticides and agricultural polyethylene soil films MPs, a comparative study on the adsorption behavior and mechanism of four pesticides (carbendazim, diflubenzuron, malathion, difenoconazole) by pristine PE MPs and APE MPs were carried out in this paper. The results showed microcracks and surface oxidation observed on APE MPs. The adsorption kinetics and isotherm models indicated that the adsorption capacity of APE MPs was higher than that of PE MPs, which attribute to the larger surface area of APE MPs. The adsorption capacities of pesticides on APE MPs were positively correlated with LogK (Water octanol partition coefficient) values of these four pesticides, showed the hydrophobic partitioning played the most important part in the adsorption, but also some H-bonding between secondary amines in the molecular of diflubenzuron and polar O-containing functional groups on APE MPs may be formed. And electrostatic forces or interactions are not the determining factor for these pesticides adsorption behavior of PE MPs, and the effect of pH is mainly driven by changes in sorbate properties rather than changes in surface properties of MPs. The results presented herein show the APE MPs can be a better vector of most hydrophobic pesticides than pristine MPs in the agricultural field, and more attention should be paid to the problem of films and pesticides residue in farmland soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.111781DOI Listing
February 2021

Efficacy of video-assisted anal fistula treatment combined with closure of the internal opening using a stapler for Parks II anal fistula.

Ann Transl Med 2020 Nov;8(22):1517

Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Background: To explore the efficacy of video-assisted anal fistula treatment (VAAFT) combined with an internal-opening closure technique using a stapler in the treatment of Parks II anal fistula.

Methods: From September 2017 to June 2019, 75 patients with Parks II anal fistulas in Beijing Erlonglu Hospital were enrolled and randomly allocated into two groups. The 37 patients in the treatment group received VAAFT combined with internal-opening closure techniques, and the 38 patients in the control group were treated with anal fistulotomy with seton placement. The primary outcomes included the healing rate and recurrence, the fecal incontinence severity index (FISI) score, and the Wexner incontinence score during the 6-month postoperative follow-up.

Results: Thirty-two cases were healed in the treatment group (86.5%) and thirty-six cases were cured in the control group (94.7%). There was no notable difference in the healing rate between the two groups (P=0.487). Significant differences between the groups were observed in the Wexner incontinence and FISI scores at 1, 3, and 6 months after the operation (P=0.001). Furthermore, the wound healing time in the treatment group was significantly shorter than in the control group (P<0.05), while the numerical rating scale (NRS) for postoperative pain on the first day and 1 week after the operation were significantly lower in the treatment group than in the control group (P<0.05).

Conclusions: VAAFT combined with closure of the internal opening using a stapler is effective and safe for Parks II anal fistula, and should be promoted as a promising treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-7154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729315PMC
November 2020

Simultaneous resection of colorectal cancer and synchronous liver metastases is safe for properly selected elderly patients: A retrospective study.

J BUON 2020 Sep-Oct;25(5):2192-2198

Department of General Surgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.

Purpose: The present study was conducted to evaluate the efficacy and safety of simultaneous resection of colorectal cancer (CRC) and synchronous liver metastases (SCRLM) in a group of elderly Chinese patients regarding the population aging in China.

Methods: From January 1st 2010 to May 1st 2015, 24 out of 32 elderly patients who underwent simultaneous resection of CRC and SCRLM were matched with 24 out of 55 young patients based on the propensity scores. Perioperative results and survival outcomes were compared.

Results: The demographic and cancer characteristics were comparable between the two groups. The postoperative duration of intensive nursing care in the elderly group was significantly longer than that in the young group [5.00 (4.00-6.75) vs. 6.50 (5.00-9.00) days, p=0.038]. No significant between-group difference was observed with respect to time to first defecation, length of postoperative hospital stay, or postoperative complication rate. There was no significant difference with respect to 3-year overall and disease-free survival rates between the two groups.

Conclusions: Simultaneous resection of CRC and SCRLM was safe and feasible in elderly patients, with reasonable 3-year survival rates. Age per se should not be considered as a contraindication for simultaneous resection of CRC and SCRLM.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2020

Solution-Processed All-Ceramic Plasmonic Metamaterials for Efficient Solar-Thermal Conversion over 100-727 °C.

Adv Mater 2021 Jan 25;33(1):e2005074. Epub 2020 Nov 25.

Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, 999077, China.

Low-cost and large-area solar-thermal absorbers with superior spectral selectivity and excellent thermal stability are vital for efficient and large-scale solar-thermal conversion applications, such as space heating, desalination, ice mitigation, photothermal catalysis, and concentrating solar power. Few state-of-the-art selective absorbers are qualified for both low- (<200 °C) and high-temperature (>600 °C) applications due to insufficient spectral selectivity or thermal stability over a wide temperature range. Here, a high-performance plasmonic metamaterial selective absorber is developed by facile solution-based processes via assembling an ultrathin (≈120 nm) titanium nitride (TiN) nanoparticle film on a TiN mirror. Enabled by the synergetic in-plane plasmon and out-of-plane Fabry-Pérot resonances, the all-ceramic plasmonic metamaterial simultaneously achieves high, full-spectrum solar absorption (95%), low mid-IR emission (3% at 100 °C), and excellent stability over a temperature range of 100-727 °C, even outperforming most vacuum-deposited absorbers at their specific operating temperatures. The competitive performance of the solution-processed absorber is accompanied by a significant cost reduction compared with vacuum-deposited absorbers. All these merits render it a cost-effective, universal solution to offering high efficiency (89-93%) for both low- and high-temperature solar-thermal applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202005074DOI Listing
January 2021

Neurofibromatosis type I in children: a case report and literature review.

Int J Clin Exp Pathol 2020 1;13(10):2656-2660. Epub 2020 Oct 1.

Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, Anhui, P. R. China.

Neurofibromatosis is an autosomal dominant genetic disease that originates from neuroepithelial tissue and involves disorders of ectoderm and mesoderm. At present, there are relatively few reports of neurofibroma type I in children. Therefore, understanding the clinical manifestations, diagnosis, and treatment of neurofibromatosis is of great significance to the occurrence and development of neurofibroma type I. This study is a case of and literature review of neurofibromatosis type I in children.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642698PMC
October 2020

Photodetection and Safety of 5-Aminolevulinic Acid-Induced Porphyrin in Patients With Cervical Intraepithelial Neoplasia.

Lasers Surg Med 2020 Nov 8. Epub 2020 Nov 8.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, 100034, China.

Background And Objectives: 5-Aminolevulinic acid (5-ALA) is a porphyrin-based photosensitizer and has been used for photodynamic therapy of cervical intraepithelial neoplasia (CIN). In this study, photodetection and safety of 5-ALA-induced porphyrin in CIN tissues were assessed.

Study Design/materials And Methods: A total of 42 patients for whom colposcopy or loop electrosurgical excision procedure (LEEP) was planned were administered 10%, 20%, or 30% 5-ALA locally on the surface of the cervix for 1.5-18 hours. At different times after application, the fluorescence intensity of 5-ALA-induced porphyrin on CIN and non-CIN lesions of the cervix was detected.

Results: Fluorescence intensity was correlated with drug concentration and application time. With 20% 5-ALA, the porphyrin fluorescence intensity increased over time, reaching a peak after 6 hours of application intervals in CIN. There was no significant difference in fluorescence intensity between CIN lesions and chronic cervicitis (benign tissue) or among CIN lesions of different severities. Three adverse events were considered related to the drug; however, these had no significant correlation with the drug concentration or application time.

Conclusions: 5-ALA at 10%-30% concentration is safe in patients with CIN. For further treatment, we recommend the application of 20% 5-ALA, 6 hours before photodynamic therapy in CIN.

Clinical Trial Registration: This study was registered at http://www.chinadrugtrials.org.cn (registration number: CTR20130326) in accordance with the requirements of the National Medical Products Administration in China. Considering that there is no English version of the above website, we made a retrospective registration on http://www.chictr.org.cn (registration number ChiCTR1800016755) in 2018. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/lsm.23338DOI Listing
November 2020

Risk Factors of Lymph Node Metastasis in Patients with Pancreatic Neuroendocrine Tumors (PNETs).

Gastroenterol Res Pract 2020 29;2020:1946156. Epub 2020 Sep 29.

Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Background: The prognostic value of lymph node metastasis in patients with PNETs is controversial. Understanding the effect of lymph node metastasis on prognosis in pancreatic neuroendocrine tumors is helpful for surgery and follow-up. The purposes of this study are to identify predictors of lymph node metastasis among patients with PNETs and determine its prognostic associations.

Methods: A retrospective analysis of the surveillance, epidemiology, and end results (SEER) database was performed. Patients with PNETs that underwent surgery and pathologic nodal staging were identified. Logistic regression and Cox regression were performed to identify independent predictors and prognostic factors, respectively.

Results: Of 1956 patients (age: 56.8 ± 13.4 years, 53.3% males), 748 (38.2%) had lymph node metastasis. On multivariable analysis, tumor located in pancreas head, distant metastasis, and poorly differentiated, undifferentiated, and unknown differentiated histology grades were three independent risk factors of lymph node metastasis. In the entire cohort, lymph node metastasis indicated a worse overall survival (HR: 1.48, 95% CI: 1.17-1.88, < 0.001) and disease-specific survival (HR: 1.87, 95% CI: 1.41-2.48, < 0.001) on multivariable analysis. Lymph node metastasis was associated with worse overall (HR: 1.45, 95% CI: 1.08-1.93, = 0.012) and disease-specific survival (HR: 2.13, 95% CI: 1.48-3.05, < 0.001) in patients without distant metastasis on multivariate analysis. Lymph node metastasis was also independently associated with worse disease-specific survival among patients in well differentiation (HR: 2.16, 95% CI: 1.35-3.46, = 0.001) and moderately differentiation (HR: 2.67, 95% CI: 1.28-5.56, = 0.009) groups on multivariate analysis.

Conclusions: Tumor located in pancreas head, distant metastasis, and poorly differentiated, undifferentiated, and unknown differentiated histology grades were three independent risk factors for lymph node metastasis. Lymph node metastasis was an independent prognostic factor of worse OS and DSS in patients with tumor located in pancreas head. Lymph node metastasis was an independent prognostic factor of worse OS and DSS in patients without distant metastasis. Lymph node metastasis was an independent prognostic factor of worse DSS in well differentiation and moderately differentiation groups.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/1946156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542491PMC
September 2020

Circulating Ceramide: A New Cardiometabolic Biomarker in Patients With Comorbid Acute Coronary Syndrome and Type 2 Diabetes Mellitus.

Front Physiol 2020 15;11:1104. Epub 2020 Sep 15.

The Second Medical Centre, Chinese PLA General Hospital, National Clinical Research Center for Geriatric Diseases, Beijing, China.

Aims: This study investigated the association of circulating ceramides in patients with comorbid acute coronary syndrome and type 2 diabetes mellitus (ACS-DM).

Methods: A total of 761 patients with coronary heart disease who were admitted to the Department of Cardiology at the Chinese PLA General Hospital from March to August 2018 were enrolled in this study. Of these 761 patients, 282 were diagnosed with acute coronary syndrome (ACS). We selected 65 patients with ACS-DM (ACS-DM group; mean age 64.88 years; 38 men) and 65 patients with ACS but without any comorbidities (ACS group; mean age 64.68 years; 38 men); the two groups were matched by age and sex. We determined four circulating ceramides in 130 plasma samples: Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/24:1), and Cer(d18:1/24:0). The ceramides in plasma samples from patients with ACS and those from patients with ACS-DM were compared. Pearson correlation coefficients between individual ceramides and traditional cardiovascular risk factors for the whole study population were calculated. Multiple logistic regression models were used to evaluate the relativity between the ceramide and ACS-DM.

Results: Compared with the ACS group, the levels of Cer(d18:1/16:0), Cer(d18:1/18:0), and Cer(d18:1/24:1) and their ratios to Cer(d18:1/24:0) were higher in the ACS-DM group and Cer(d18:1/24:0) was lower in the ACS-DM group ( < 0.05). Correlation analysis demonstrated mild-to-moderate correlations of ceramide and traditional cardiovascular risk factors. There were relatively strong correlations of Cer(d18:1/18:0) and Cer(d18:1/24:1) with C-reactive protein, blood lipids, fasting blood glucose, and glycated hemoglobin Ac. In multiple logistic regression models, Cer(d18:1/18:0) [odds ratio (OR) 2.396; 95% confidence interval (CI) 1.103-5.205; = 0.027], Cer(d18:1/24:1) (OR 2.826; 95% CI 1.158-6.896; = 0.023), Cer(d18:1/18:0)/Cer(d18:1/24:0) (OR 2.242; 95% CI 1.103-4.555; = 0.026), and Cer(d18:1/24:1)/Cer(d18:1/24:0) (OR 2.673; 95% CI 1.225-5.836; = 0.014) were positively correlated with ACS-DM, and Cer(d18:1/24:0) (OR 0.200; 95% CI 0.051-0.778; = 0.020) was negatively correlated with ACS-DM.

Conclusion: Circulating ceramides are positively correlated with the risk of ACS-DM comorbidity. These results give a new insight into the pathogenesis of ACS-DM comorbidity and could provide new options for risk estimation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2020.01104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522524PMC
September 2020

[Isochlorogenic acid A inhibits proliferation and migration and promotes apoptosis in MH7A cells induced by TNF-α].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Aug;36(8):693-698

Department of Rheumatology and Immunology, First Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China. *Corresponding author,

Objective To investigate the effects of isochlorogenic acid A on the proliferation, migration, invasion and apoptosis of MH7A cells. Methods Following by the induction of 20 μg/L TNF-α in vitro, different concentrations of isochlorogenic acid A (0, 0.04, 0.09, 0.13, 0.16, 0.20 μg/μL) were added into MH7A cells. The proliferation of MH7A cells was detected by CCK-8 assay, and the invasion and migration were observed by Transwell assay. The levels of cellular caspase-3, ROS and MMP3 in the MH7A cells were detected by corresponding kits. BAX and Bcl2 expression of MH7A cells were tested by immunofluorescence cytochemistry. Results The proliferation, invasion and migration activity were enhanced significantly and the apoptotic activity was reduced in MH7A cells induced by 20 μg/L TNF-α. Compared with TNF-α in vitro induction, isochlorogenic acid A significantly inhibited the proliferation, invasion, migration and MMP3 secretion of MH7A cells, increased ROS release and promoted MH7A apoptosis. Conclusion Isochlorogenic acid A can inhibit proliferation, invasion, migration and promoted apoptosis of MH7A cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2020

Myosin light chain kinase is a potential target for hypopharyngeal cancer treatment.

Biomed Pharmacother 2020 Nov 10;131:110665. Epub 2020 Sep 10.

Laboratory of Molecular Biology, and Department of Biochemistry, Anhui Medical University, Hefei, Anhui, 230032, China; Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, 230032, China; Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, United States. Electronic address:

Hypopharyngeal cancer is squamous cell carcinoma (SCC) with the worst prognosis among the head and neck cancers. Overall, the 5-year survival rate remains poor although diagnostic imaging, radiation, chemotherapy, and surgical techniques have been improved. The mortality of patients with hypopharyngeal cancer is partly due to an increased likelihood of developing a second primary malignancy and metastasis. In this study, we found that MLCK expression, compared to healthy tissue, was up-regulated in hypopharyngeal tumor tissue. Of particular interest, a low 5-year survival rate was positively correlated with MLCK expression. We hypothesized that MLCK might be a target for hypopharyngeal cancer prognosis and treatment. In order to explore the function of MLCK in the development of cancer, we knockdown MLCK in hypopharyngeal cancer FaDu cells. The results showed that MLCK knockdown reduced the migration and invasion of FaDu cells. 4-amino-2-trifluoromethyl-phenyl retinate (ATPR) is the derivative of all-trans retinoic acid (ATRA), which was able to reduce both MLCK expression and activity in FaDu cells. ATPR induced FaDu cells apoptosis in a dose-dependent manner and also inhibited cell growth both in vivo and in vitro. Further experiments showed that overexpression of MLCK reduced ATPR induced-migration inhibition while increase of ATPR induced apoptosis, which suggested that MLCK was involved in ATPR's anti-cancer function. In conclusion, MLCK is a novel prognostic marker and therapeutic target for hypopharyngeal cancer. By targeting MLCK, ATPR exhibits its potential application in the treatment of this type of cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.110665DOI Listing
November 2020

Elabela alleviates myocardial ischemia reperfusion-induced apoptosis, fibrosis and mitochondrial dysfunction through PI3K/AKT signaling.

Am J Transl Res 2020 15;12(8):4467-4477. Epub 2020 Aug 15.

National Clinical Research Center for Geriatric Diseases, 2nd Medical Center, Chinese PLA General Hospital Beijing 100853, China.

Myocardial ischemia/reperfusion (I/R) injury is a common cardiovascular disease with high morbidity and mortality globally, which derives from acute myocardial infarction and coronary artery disease. Elabela has been proved to bind to apelin receptors in the heart. The present study aimed to investigate the protective effects of Elabela in myocardial I/R injury and illustrating the potential mechanisms. In this study, the rat I/R model was established . Following treatment with Elabela, the histopathological changes of heart tissue were evaluated by the hematoxylin and eosin- or Masson's trichrome staining. Apoptosis of heart tissue was examined using TUNEL staining. The expression of type I or III collagen and apoptosis-associated proteins was measured using western blotting. Moreover, myocardial ultrastructure in myocardium was detected via electron microscopy analysis. H9c2 cells were treated with hypoxia/reoxygenation (H/R) to mimic the myocardial I/R injury . After treatment with Elabela or Elabela combined with LY294002, the levels of oxidative stress and apoptosis were examined. The results revealed that Elabela significantly improved the pathological changes of rat myocardial tissues induced by I/R. Additionally, Elabela treatment reduced cardiomyocyte I/R induced fibrosis and apoptosis as well as ameliorated mitochondrial dysfunction in animal and cells. Within inhibition of PI3K pathway, the protective effects of Elabela was reversed. Taken together, these findings demonstrated that Elabela could protect against fibrosis, apoptosis and oxidative stress via PI3K/ATK signaling pathway in cardiac ischemia reperfusion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476165PMC
August 2020

Laparoscopic advanced intraoperative restaging for radiographic non-metastasis pancreatic cancer: Protocol for a single-center, cross-sectional and follow-up study.

Medicine (Baltimore) 2020 Sep;99(36):e22090

Department of General Surgery, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China.

Background: Although surgical resection holds promise for curing pancreatic cancer, <20% of patients are suitable; however, early postoperative recurrence is common. Currently, radiographic examination is the primary method to determine whether pancreatic cancer has metastasized and to inform clinical staging before surgery. However, the method has a limited detection rate for micro-metastasis within the abdominal cavity; therefore, patients with advanced pancreatic cancer and existing micro-metastasis may receive unnecessary surgical treatment, delaying the timing of adjuvant chemotherapy and resulting in poor prognosis. Laparoscopic staging might be used as a supplement to detect micro-metastasis in patients with pancreatic cancer; however, there is no consistent standard to guide the use of this procedure. Therefore, it is necessary to conduct a trial to further explore the consistency and short-term and long-term efficacy of an intraoperative staging strategy for patients with radiographic non-metastasis.

Methods/design: This is a single-center cross-sectional and follow-up study. Patients diagnosed with pancreatic cancer without metastasis by radiographic examination and histopathological biopsy, who received intraoperative restaging, will be enrolled. The total sample size required for the trial is approximately 125 patients from May 2020 to December 2022. First, radiographic examination staging will be used. Then, laparoscopic exploration will be performed for patients without definite metastatic lesions. Data collection will include preoperative blood examination, radiographic examination, surgical information, and postoperative recovery. The patients will undergo follow-up every 3 months after surgery until death. The primary endpoint is the metastasis-positive rate via laparoscopic exploration. The secondary endpoints are the consistency, sensitivity, and specificity of the intraoperative restaging strategy and radiographic examination, the incidence of postoperative complications within 30 days, the 6-month relapse-free survival rate, and perioperative indicators (total cost, hospital stay, length of surgery, and intraoperative blood loss).

Discussion: We are conducting the trial to explore the metastasis-positive rate of intraoperative restaging strategy for diagnosing pancreatic cancer micro-metastasis. This new intraoperative restaging strategy would help pancreatic cancer patients with potential micro-metastasis avoid receiving unnecessary resection, allow systemic treatment as early as possible, and improve the prognosis of patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000022090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478394PMC
September 2020

Lightweight and Hydrophobic Three-Dimensional Wood-Derived Anisotropic Magnetic Porous Carbon for Highly Efficient Electromagnetic Interference Shielding.

ACS Appl Mater Interfaces 2020 Sep 27;12(36):40802-40814. Epub 2020 Aug 27.

Key Laboratory for Anisotropy and Texture of Materials (MOE), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, P. R. China.

Constructing multifunctional characteristics toward advanced electromagnetic interference shielding materials in harsh environments has become a development trend. Herein, the wood-derived magnetic porous carbon composites with a highly ordered anisotropic porous architecture were successfully fabricated through a pyrolysis procedure. The three-dimensional porous skeleton inherited from the wood stock serves as an electrically conductive network and incorporates magnetic Ni nanoparticles homogeneously and firmly embedded within the carbon matrix that can further improve the electromagnetic attenuation capacity. The optimized Ni/porous carbon (PC) composite exhibits an exceptional electromagnetic interference (EMI) shielding effectiveness of 50.8 dB at the whole X band (8.2-12.4 GHz) with a low thickness (2 mm) and an ultralow density (0.288 g/cm) and simultaneously possesses an extraordinary compressive strength (11.7 MPa) and a hydrophobic water contact angle (152.1°). Our study provides an alternative strategy to utilize green wood-based materials to design multifunctional EMI shielding composites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c11530DOI Listing
September 2020

A Prognostic Nomogram Based on Immune Scores Predicts Postoperative Survival for Patients with Hepatocellular Carcinoma.

Biomed Res Int 2020 7;2020:1542394. Epub 2020 Jul 7.

Department of General Surgery, Xuanwu Hospital of Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, China.

Background: Increasing research attention has focused on tumor-infiltrating immune cells. However, the threshold of an immune score for use in predicting overall survival (OS) and disease-free survival (DFS) in hepatocellular carcinoma (HCC) is not defined. This study aims at exploring the association between immune scores with prognosis and building a clinical nomogram for predicting the survival of HCC patients. . A total of 299 patients were enrolled in this study. Their clinical pathological characteristics and immune scores downloaded from The Cancer Genome Atlas (TCGA) database were analyzed. Survival differences between different immune score subgroups were compared, and a final nomogram was built using the Cox proportional hazards regression model. The predictive performance of the nomogram was assessed using the concordance index (C-index) and a calibration plot.

Results: All the patients were divided into three subgroups based on immune scores. Patients with medium and high immune scores had significantly better OS (HR and 95% CI: 0.417 [0.186-0.937] and 0.299 [0.146-0.616]) and DFS (HR and 95% CI: 0.575 [0.329-1.004] and 0.451 [0.278-0.733], respectively, compared with those with low immune scores. The C indices for OS and DFS were 0.748 (95% CI, 0.687-0.809) and 0.675 (95% CI, 0.630-0.720), respectively. A calibration plot used to determine the probability of survival at 3 or 5 years (OS and DFS) showed a significant agreement between nomogram predictions and actual observations.

Conclusions: Medium and high immune scores are significantly associated with prolonged OS and DFS in HCC patients. Nomograms built in this study can help doctors and patients assess prognosis and guide treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/1542394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366168PMC
July 2020

Neuroligin 2 regulates absence seizures and behavioral arrests through GABAergic transmission within the thalamocortical circuitry.

Nat Commun 2020 07 27;11(1):3744. Epub 2020 Jul 27.

Neuroscience & Mental Health, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.

Epilepsy and autism spectrum disorders (ASD) are two distinct brain disorders but have a high rate of co-occurrence, suggesting shared pathogenic mechanisms. Neuroligins are cell adhesion molecules important in synaptic function and ASD, but their role in epilepsy remains unknown. In this study, we show that Neuroligin 2 (NLG2) knockout mice exhibit abnormal spike and wave discharges (SWDs) and behavioral arrests characteristic of absence seizures. The anti-absence seizure drug ethosuximide blocks SWDs and rescues behavioral arrests and social memory impairment in the knockout mice. Restoring GABAergic transmission either by optogenetic activation of the thalamic reticular nucleus (nRT) presynaptic terminals or postsynaptic NLG2 expression in the thalamic neurons reduces the SWDs and behavioral arrests in the knockout mice. These results indicate that NLG2-mediated GABAergic transmission at the nRT-thalamic circuit represents a common mechanism underlying both epileptic seizures and ASD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-17560-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385104PMC
July 2020

Taxonomic studies on the genus (Aristolochiaceae) from China: II. (Aristolochiaceae), a new species from Guizhou, China.

PhytoKeys 2020 3;152:15-25. Epub 2020 Jul 3.

College of Life Sciences, Xinyang Normal University, Xinyang, 464000, Henan, China Xinyang Normal University Xinyang China.

A new species of was recently discovered from Guizhou, China and is here named as . It is most similar to and , but differs in the morphology of leaves and flowers. A detailed description for the new species, along with line drawings, photographs, as well as morphological comparisons with similar species, are provided. In addition, the distribution of in China is here confirmed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/phytokeys.152.51760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351795PMC
July 2020

Integrated epigenetic biomarkers in circulating cell-free DNA as a robust classifier for pancreatic cancer.

Clin Epigenetics 2020 07 23;12(1):112. Epub 2020 Jul 23.

Department of Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.

Background: The high lethal rate of pancreatic cancer is partly due to a lack of efficient biomarkers for screening and early diagnosis. We attempted to develop effective and noninvasive methods using 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) markers from circulating cell-free DNA (cfDNA) for the detection of pancreatic ductal adenocarcinoma (PDAC).

Results: A 24-feature 5mC model that can accurately discriminate PDAC from healthy controls (area under the curve (AUC) = 0.977, sensitivity = 0.824, specificity = 1) and a 5hmC prediction model with 27 features demonstrated excellent detection power in two distinct validation sets (AUC = 0.992 and 0.960, sensitivity = 0.786 and 0.857, specificity = 1 and 0.993). The 51-feature model combining 5mC and 5hmC markers outperformed both of the individual models, with an AUC of 0.997 (sensitivity = 0.938, specificity = 0.955) and particularly an improvement in the prediction sensitivity of PDAC. In addition, the weighted diagnosis score (wd-score) calculated with the 5hmC model can distinguish stage I patients from stage II-IV patients.

Conclusions: Both 5mC and 5hmC biomarkers in cfDNA are effective in PDAC detection, and the 5mC-5hmC integrated model significantly improve the detection sensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13148-020-00898-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376965PMC
July 2020

Rapamycin Promotes Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells in a Stage-Dependent Manner.

Stem Cells Dev 2020 09 18;29(18):1229-1239. Epub 2020 Aug 18.

Department of Cardiology & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital & Medical School of Chinese PLA, Beijing, China.

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are a promising source for cardiac regenerative therapy, and ideal for in vitro cell modeling of cardiovascular diseases and drug screening. Recent studies have shown that rapamycin can promote cardiomyocyte differentiation in various stem cells. However, how rapamycin affects cardiomyocyte differentiation of iPSCs is still not fully understood. This study aimed to investigate the effect of rapamycin on cardiomyocyte differentiation based on embryoid body (EB) method. First, to determine the autophagy induction protocol, different concentrations of rapamycin were applied in hEBs on day 6. The autophagy was most significant when applying rapamycin at 1 μM for 48 h, demonstrating by the LC3II/LC3I ratio and p62 expression. Then, 1 μM rapamycin was applied for 48 h at different time points of cardiomyocyte differentiation to investigate the role of rapamycin in this process. Compared with control, rapamycin applied on days 0-4 of differentiation significantly decreased the proportion of beating EBs and expression of cardiomyocyte-specific genes, while rapamycin applied on days 4-14 significantly increased them. Among all groups, rapamycin applied on days 4-6 achieved highest cardiomyocyte differentiation efficiency. Furthermore, using autophagy inhibitor NHCl and GSK-3β inhibitor CHIR-99021, we found rapamycin-induced autophagy promoted cardiomyocyte differentiation at middle stage by negatively regulating the Wnt/β-catenin signaling pathway. These results suggest that rapamycin regulates EB-based cardiomyocyte differentiation in a stage-dependent manner, and the negative regulation of Wnt/β-catenin signaling pathway by autophagy was involved in the prodifferentiation effect of rapamycin at middle stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/scd.2020.0025DOI Listing
September 2020