Publications by authors named "Feng Bai"

247 Publications

Self-reference Network-Related Interactions During the Process of Cognitive Impairment in the Early Stages of Alzheimer's Disease.

Front Aging Neurosci 2021 24;13:666437. Epub 2021 Mar 24.

Department of Neurology, Affiliated Drum Tower Hospital of Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

: Normal establishment of cognition occurs after forming a sensation to stimuli from internal or external cues, in which self-reference processing may be partially involved. However, self-reference processing has been less studied in the Alzheimer's disease (AD) field within the self-reference network (SRN) and has instead been investigated within the default-mode network (DMN). Differences between these networks have been proven in the last decade, while ultra-early diagnoses have increased. Therefore, investigation of the altered pattern of SRN is significantly important, especially in the early stages of AD. : A total of 65 individuals, including 43 with mild cognitive impairment (MCI) and 22 cognitively normal individuals, participated in this study. The SRN, dorsal attention network (DAN), and salience network (SN) were constructed with resting-state functional magnetic resonance imaging (fMRI), and voxel-based analysis of variance (ANOVA) was used to explore significant regions of network interactions. Finally, the correlation between the network interactions and clinical characteristics was analyzed. : We discovered four interactions among the three networks, with the SRN showing different distributions in the left and right hemispheres from the DAN and SN and modulated interactions between them. Group differences in the interactions that were impaired in MCI patients indicated that the degree of damage was most severe in the SRN, least severe in the SN, and intermediate in the DAN. The two SRN-related interactions showed positive effects on the executive and memory performances of MCI patients with no overlap with the clinical assessments performed in this study. : This study is the first and primary evidence of SRN interactions related to MCI patients' functional performance. The influence of the SRN in the ultra-early stages of AD is nonnegligible. There are still many unknowns regarding the contribution of the SRN in AD progression, and we strongly recommend future research in this area.
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http://dx.doi.org/10.3389/fnagi.2021.666437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024683PMC
March 2021

Noncovalent Self-Assembled Smart Gold(III) Porphyrin Nanodrug for Synergistic Chemo-Photothermal Therapy.

Nano Lett 2021 Apr 7. Epub 2021 Apr 7.

Key Laboratory for Special Functional Materials of Ministry of Education, National and Local Joint Engineering Research Center for High-Efficiency Display and Lighting Technology, School of Materials Science and Engineering, Collaborative Innovation Center of Nano Functional Materials and Applications, Henan University, Kaifeng 475004, China.

Self-assembly is a powerful means to fabricate multifunctional smart nanotheranostics. However, the complicated preparation, toxicity of responsive carriers, and low loading efficiency of drug cargo hinder the outcome. Herein, we developed a responsive carrier-free noncovalent self-assembly strategy of a metallized Au(III) tetra-(4-pyridyl) porphine (AuTPyP) anticancer drug for the preparation of a heat/acid dual-stimulated nanodrug, and it generated a better photothermal effect than monomers under irradiation. The photothermal effect promoted the protonation of the hydrophobic pyridyl group and the following release into tumorous acidic microenvironments. With cRGD modification, the released drug induced the aggravation of intracellular reactive oxygen species (ROS) via the activity inhibition of thioredoxin reductase (TrxR) for synergistic chemo-photothermal therapy of tumors.
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http://dx.doi.org/10.1021/acs.nanolett.0c04915DOI Listing
April 2021

Brain Structural Network Compensation Is Associated With Cognitive Impairment and Alzheimer's Disease Pathology.

Front Neurosci 2021 25;15:630278. Epub 2021 Feb 25.

Department of Neurology, Affiliated Drum Tower Hospital of Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

Background: Structural network alterations in Alzheimer's disease (AD) are related to worse cognitive impairment. The aim of this study was to quantify the alterations in gray matter associated with impaired cognition and their pathological biomarkers in AD-spectrum patients.

Methods: We extracted gray matter networks from 3D-T1 magnetic resonance imaging scans, and a graph theory analysis was used to explore alterations in the network metrics in 34 healthy controls, 70 mild cognitive impairment (MCI) patients, and 40 AD patients. Spearman correlation analysis was computed to investigate the relationships among network properties, neuropsychological performance, and cerebrospinal fluid pathological biomarkers (i.e., Aβ, t-tau, and p-tau) in these subjects.

Results: AD-spectrum individuals demonstrated higher nodal properties and edge properties associated with impaired memory function, and lower amyloid-β or higher tau levels than the controls. Furthermore, these compensations at the brain regional level in AD-spectrum patients were mainly in the medial temporal lobe; however, the compensation at the whole-brain network level gradually extended from the frontal lobe to become widely distributed throughout the cortex with the progression of AD.

Conclusion: The findings provide insight into the alterations in the gray matter network related to impaired cognition and pathological biomarkers in the progression of AD. The possibility of compensation was detected in the structural networks in AD-spectrum patients; the compensatory patterns at regional and whole-brain levels were different and the clinical significance was highlighted.
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http://dx.doi.org/10.3389/fnins.2021.630278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947929PMC
February 2021

Hyperconnectivity of Self-Referential Network as a Predictive Biomarker of the Progression of Alzheimer's Disease.

J Alzheimers Dis 2021 ;80(2):577-590

Department of Neurology, Nanjing Drum Tower Hospital of The Affiliated Hospital of Nanjing University Medical School, and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

Background: Self-referential processing is associated with the progression of Alzheimer's disease (AD), and cerebrospinal fluid (CSF) proteins have become accepted biomarkers of AD.

Objective: Our objective in this study was to focus on the relationships between the self-referential network (SRN) and CSF pathology in AD-spectrum patients.

Methods: A total of 80 participants, including 20 cognitively normal, 20 early mild cognitive impairment (EMCI), 20 late MCI (LMCI), and 20 AD, were recruited for this study. Independent component analysis was used to explore the topological SRN patterns, and the abnormalities of this network were identified at different stages of AD. Finally, CSF pathological characteristics (i.e., CSF Aβ, t-tau, and p-tau) that affected the abnormalities of the SRN were further determined during the progression of AD.

Results: Compared to cognitively normal subjects, AD-spectrum patients (i.e., EMCI, LMCI, and AD) showed a reversing trend toward an association between CSF pathological markers and the abnormal SRN occurring during the progression of AD. However, a certain disease state (i.e., the present LMCI) with a low concentration of CSF tau could evoke more hyperconnectivity of the SRN than other patients with progressively increasing concentrations of CSF tau (i.e., EMCI and AD), and this fluctuation of CSF tau was more sensitive to the hyperconnectivity of the SRN than the dynamic changes of CSF Aβ.

Conclusion: The integrity of the SRN was closely associated with CSF pathological characteristics, and these findings support the view that the hyperconnectivity of the SRN will play an important role in monitoring the progression of the pre-dementia state to AD.
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http://dx.doi.org/10.3233/JAD-201376DOI Listing
January 2021

Cu-MOF Material Constructed with a Triazine Polycarboxylate Skeleton: Multifunctional Identify and Microdetecting of the Aromatic Diamine Family (,,-Phenylenediamine) Based on the Luminescent Response.

Inorg Chem 2021 Feb 27;60(4):2829-2838. Epub 2021 Jan 27.

Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, Guilin 541004, PR China.

Organic aromatic amines are widely used in various fields such as pharmaceuticals, pesticides, dyes, and tobacco smoke. The pollution of organic amines has become a problem that cannot be ignored, due to the extensive harmful effects on the environment and public health, which has become one of the most concerned frontier fields in the world. Identifying and microdetecting -phenylenediamine (OPD), -phenylenediamine (MPD), and -phenylenediamine (PPD) using MOFs have rarely been reported. On the basis of the blue emission properties of Cu-TBDA constructed with 5,5'-((6-chloro-1,3,5-triazine-2,4-diyl)bis(azanediyl))diisophthalic acid (HTBDA) ligand, Cu-TBDA was studied primarily to identify and detect aromatic diamine family as a multifunctional chemical sensor. Interestingly, Cu-TBDA has a very high selectivity and sensitivity to OPD and MPD with a low limit of detection (5.00 μM for OPD and 1.77 μM for MPD). Especially for OPD, Cu-TBDA has a unique switching function for it. When the concentration of OPD is less than 9.1 × 10 M, the fluorescence response of Cu-TBDA suspension exhibit enhanced. However, when the concentration of OPD is more than 9.1 × 10 M, the emission intensity displays quenching phenomenon. Therefore, Cu-TBDA as a chemical sensor not only has recognition and detection functions for organic aromatic amines but also first exhibits turn-on and -off sensing behavior toward OPD.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03753DOI Listing
February 2021

PDGFRβ is an essential therapeutic target for BRCA1-deficient mammary tumors.

Breast Cancer Res 2021 Jan 21;23(1):10. Epub 2021 Jan 21.

Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, International Cancer Center, Shenzhen University Health Science Center, Shenzhen, 518060, China.

Background: Basal-like breast cancers (BLBCs) are a leading cause of cancer death due to their capacity to metastasize and lack of effective therapies. More than half of BLBCs have a dysfunctional BRCA1. Although most BRCA1-deficient cancers respond to DNA-damaging agents, resistance and tumor recurrence remain a challenge to survival outcomes for BLBC patients. Additional therapies targeting the pathways aberrantly activated by BRCA1 deficiency are urgently needed.

Methods: Most BRCA1-deficient BLBCs carry a dysfunctional INK4-RB pathway. Thus, we created genetically engineered mice with Brca1 loss and deletion of p16, or separately p18, to model the deficient INK4-RB signaling in human BLBC. By using these mutant mice and human BRCA1-deficient and proficient breast cancer tissues and cells, we tested if there exists a druggable target in BRCA1-deficient breast cancers.

Results: Heterozygous germline or epithelium-specific deletion of Brca1 in p18- or p16-deficient mice activated Pdgfrβ signaling, induced epithelial-to-mesenchymal transition, and led to BLBCs. Confirming this role, targeted deletion of Pdgfrβ in Brca1-deficient tumor cells promoted cell death, induced mesenchymal-to-epithelial transition, and suppressed tumorigenesis. Importantly, we also found that pharmaceutical inhibition of Pdgfrβ and its downstream target Pkcα suppressed Brca1-deficient tumor initiation and progression and effectively killed BRCA1-deficient cancer cells.

Conclusions: Our work offers the first genetic and biochemical evidence that PDGFRβ-PKCα signaling is repressed by BRCA1, which establishes PDGFRβ-PKCα signaling as a therapeutic target for BRCA1-deficient breast cancers.
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http://dx.doi.org/10.1186/s13058-021-01387-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819225PMC
January 2021

Role of lncRNA LUCAT1 in cancer.

Biomed Pharmacother 2021 Feb 24;134:111158. Epub 2020 Dec 24.

Lanzhou University Second Hospital, Department of Cardiology, 82 Cuiying Men, Lanzhou, 730030, PR China. Electronic address:

Long non-coding RNAs (lncRNAs) are RNA molecules with a transcript length of more than 200 nt and lack a protein-coding ability. They regulate gene expression by interacting with protein, RNA, and DNA. Their function is closely related to their subcellular localization. In the nucleus, lncRNAs regulate gene expression at the epigenetic and transcriptional levels, and in the cytoplasm, they regulate gene expression at the post-transcriptional and translational levels. Abnormalities in lncRNAs have been confirmed to exhibit tumor suppressor or carcinogenic effects and play an important role in the development of tumors. In particular, the lung cancer-related transcript 1 (LUCAT1) located in the antisense strand of the q14.3 region of chromosome 5 was first discovered in smoking-related lung cancer. Increasing evidence have showed that LUCAT1 is involved in breast cancer, ovarian cancer, thyroid cancer, renal cell carcinoma. It is highly expressed in liver cancer and other malignant tumors and has been confirmed to be induce various malignant tumors. It regulates tumor proliferation, invasion, and migration via various mechanisms and is related to the clinicopathological characteristics of tumor patients. Thus, LUCAT1 is a potential prognostic biological marker and therapeutic target for cancer. This article reviews its expression, function, and molecular mechanism in various malignant tumors.
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http://dx.doi.org/10.1016/j.biopha.2020.111158DOI Listing
February 2021

Long Longitudinal Tract Lesion Contributes to the Progression of Alzheimer's Disease.

Front Neurol 2020 16;11:503235. Epub 2020 Oct 16.

The State Key Laboratory of Pharmaceutical Biotechnology, Department of Neurology, Affiliated Drum Tower Hospital of Medical School, Institute of Brain Science, Nanjing University, Nanjing, China.

The degenerative pattern of white matter (WM) microstructures during Alzheimer's disease (AD) and its relationship with cognitive function have not yet been clarified. The present research aimed to explore the alterations of the WM microstructure and its impact on amnestic mild cognitive (aMCI) and AD patients. Mechanical learning methods were used to explore the validity of WM microstructure lesions on the classification in AD spectrum disease. Neuropsychological data and diffusion tensor imaging (DTI) images were collected from 28 AD subjects, 31 aMCI subjects, and 27 normal controls (NC). Tract-based spatial statistics (TBSS) were used to extract diffusion parameters in WM tracts. We performed ANOVA analysis to compare diffusion parameters and clinical features among the three groups. Partial correlation analysis was used to explore the relationship between diffusion metrics and cognitive functions controlling for age, gender, and years of education. Additionally, we performed the support vector machine (SVM) classification to determine the discriminative ability of DTI metrics in the differentiation of aMCI and AD patients from controls. As compared to controls or aMCI patients, AD patients displayed widespread WM lesions, including in the inferior longitudinal fasciculus, inferior fronto-occipital fasciculi, and superior longitudinal fasciculus. Significant correlations between fractional anisotropy (FA), mean diffusivity (MD), and radial diffusion (RD) of the long longitudinal tract and memory deficits were found in aMCI and AD groups, respectively. Furthermore, through SVM classification, we found DTI indicators generated by FA and MD parameters can effectively distinguish AD patients from the control group with accuracy rates of up to 89 and 85%, respectively. The WM microstructure is extensively disrupted in AD patients, and the WM integrity of the long longitudinal tract is closely related to memory, which would hold potential value for monitoring the progression of AD. The method of classification based on SVM and WM damage features may be objectively helpful to the classification of AD diseases.
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http://dx.doi.org/10.3389/fneur.2020.503235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597387PMC
October 2020

Aberrant White Matter Microstructure as a Potential Diagnostic Marker in Alzheimer's Disease by Automated Fiber Quantification.

Front Neurosci 2020 24;14:570123. Epub 2020 Sep 24.

Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Neuroimaging evidence has suggested white matter microstructure are heavily affected in Alzheimer's disease (AD). However, whether white matter dysfunction is localized at the specific regions of fiber tracts and whether they would be a potential biomarker for AD remain unclear. By automated fiber quantification (AFQ), we applied diffusion tensor images from 25 healthy controls (HC), 24 amnestic mild cognitive impairment (aMCI) patients and 18 AD patients to create tract profiles along 16 major white matter fibers. We compared diffusion metrics [Fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (DA), and radial diffusivity (DR)] between groups. To assess the diagnostic value, we applied a random forest (RF) classifier, a type of machine learning method. In the global tract level, we found that aMCI and AD patients showed higher MD, DA, and DR values in some fiber tracts mostly in the left hemisphere compared to HC. In the point-wise level, widespread disruption were distributed on specific locations of different tracts. The point-wise MD measurements presented the best classification performance with respect to differentiating AD from HC. The two most important variables were localized in the prefrontal potion of left uncinate fasciculus and anterior thalamic radiation. In addition, the point-wise DA in the posterior component of the left cingulum cingulate displayed the most robust discriminative ability to identify AD from aMCI. Our findings provide evidence that white matter abnormalities based on the AFQ method could be as a diagnostic biomarker in AD.
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http://dx.doi.org/10.3389/fnins.2020.570123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541946PMC
September 2020

Precise magnetic resonance imaging-guided sonodynamic therapy for drug-resistant bacterial deep infection.

Biomaterials 2021 Jan 18;264:120386. Epub 2020 Sep 18.

CAS Center for Excellence in Nanoscience, Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Beijing, 100190, China. Electronic address:

The precise treatment of drug-resistant deep bacterial infections remains a huge challenge in clinic. Herein, a polymer-peptide-porphyrin conjugate (PPPC), which can be real-time monitored in infectious site, is developed for accurate and deep sonodynamic therapy (SDT) based on "in vivo self-assembly" strategy. The PPPC contains four moieties, i.e., a hyperbranched polymer backbone, a self-assembled peptide linked with an enzyme-cleavable peptide-poly (ethylene glycol) terminal, a bacterial targeting peptide, and a porphyrin sonosensitizer (MnTCPP) segment. Once PPPC nanoparticles reach the infectious area, the protecting PEG layers are removed due to the over-expressed gelatinase, leading to the secondary assembly into large nanoaggregates and resultant enhanced accumulation of sonosensitizer. The nanoaggregates exhibit enhanced interaction with bacterial membrane and decrease the minimum inhibitory concentration (MIC) significantly. Meanwhile, compared with free MnTCPP, the concentration of which can not be accurately quantified, the accumulation amount of MnTCPP in PPPCs at infectious site can be in situ monitored by magnetic resonance imaging (MRI) using T combined with T. When the concentration of PPPC-1 reaches MIC, the drug-resistant bacterial infection area is exposed to ultrasound irradiation, causing the precise and efficient elimination of bacteria. Therefore, the MRI-guided SDT system shows extraordinary tissue penetration depth, drug concentration monitoring, morphology-transformation induced accumulation and improved treatment capacity toward drug-resistant bacteria.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120386DOI Listing
January 2021

Physical activity and liver health among urban and rural Chinese adults: results from two independent surveys.

J Exerc Sci Fit 2021 Jan 28;19(1):8-12. Epub 2020 Jul 28.

Primary Care Centre Versus Arthritis, Research Institute for Primary Care & Health Sciences, Keele University, Keele, ST5 5BG, UK.

Background: Increased physical activity has been associated with reduced risks of various physical and mental conditions. However, the association between physical activity and liver health in the Chinese general adult population is not clear. This study investigated whether physical activity, stratified by intensity (i.e. walking (light), moderate-to-vigorous), was associated with alanine aminotransferase (ALT) level in middle-aged and older Chinese adults.

Methods: Two independent surveys of urban (n = 5,824, males 44%, mean (standard deviation) age 52 (10) years) and rural populations (n = 20,269, males 41%, mean (standard deviation) age 51 (10) years) were undertaken. Physical activity was measured using the International Physical Activity Questionnaire, and in metabolic equivalents of task (MET) × minutes. Elevated serum level of ALT, a clinical surrogate of abnormal liver function, was defined as >40 IU/L (males) and >30 IU/L (females). Multivariable regression models were used.

Results: Amount of moderate-to-vigorous activity was inversely associated with serum level of ALT (β = -0.147 per 1k MET-minutes, p < 0.001), whereas walking was not associated. People who reached the lower limit of WHO recommendation (≥600 MET-minutes per week) had a reduced odds of ALT elevation, compared to those who did not (adjusted odds ratio: 0.85 95%CI (0.76, 0.95)).

Conclusions: Meeting the moderate-to-vigorous recommendations for physical activity in adults may be associated with decreased likelihood of abnormal liver function both in Chinese urban and rural populations. Promoting such activities could be a low-cost strategy in maintaining liver health as well as providing many other health-related benefits.
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http://dx.doi.org/10.1016/j.jesf.2020.07.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452301PMC
January 2021

Exogenous supplement of glucagon like peptide-1 protects the heart against aortic banding induced myocardial fibrosis and dysfunction through inhibiting mTOR/p70S6K signaling and promoting autophagy.

Eur J Pharmacol 2020 Sep 1;883:173318. Epub 2020 Jul 1.

Key Laboratory of Cellular Physiology of Ministry of Education and Department of Physiology, Shanxi Medical University, Taiyuan, Shanxi, China; Basic Biomedical Sciences, Mercer University School of Medicine, Savannah, GA, USA. Electronic address:

Mammalian target of rapamycin (mTOR) and a ribosomal protein S6 kinase (p70S6K) mediate tissue fibrosis and negatively regulate autophagy. This study aims to investigate whether glucagon-like peptide-1 (GLP-1) analog liraglutide protects the heart against aortic banding-induced cardiac fibrosis and dysfunction through inhibiting mTOR/p70S6K signaling and promoting autophagy activity. Male SD rats were randomly divided into four groups (n = 6/each group): sham operated control; abdominal aortic constriction (AAC); liraglutide treatment during AAC (0.3 mg/kg, injected subcutaneously twice daily); rapamycin treatment during AAC (0.2 mg/kg/day, administered by gastric gavage). Relative to the animals with AAC on week 16, liraglutide treatment significantly reduced heart/body weight ratio, inhibited cardiomyocyte hypertrophy, and augmented plasma GLP-1 level and tissue GLP-1 receptor expression. Phosphorylation of mTOR/p70S6K, populations of myofibroblasts and synthesis of collagen I/III in the myocardium were simultaneously inhibited. Furthermore, autophagy regulating proteins: LC3-II/LC3-I ratio and Beclin-1 were upregulated, and p62 was downregulated by liraglutide. Compared with liraglutide group, treatment with rapamycin, a specific inhibitor of mTOR, compatibly augmented GLP-1 receptor level, inhibited phosphorylation of mTOR/p70S6K and expression of p62 as well as increased level of LC3-II/LC3-I ratio and Beclin-1, suggesting that there is an interaction between GLP-1 and mTOR/p70S6K signaling in the regulation of autophagy. In line with these modifications, treatment with liraglutide and rapamycin significantly reduced perivascular/interstitial fibrosis, and preserved systolic/diastolic function. These results suggest that the inhibitory effects of liraglutide on cardiac fibrosis and dysfunction are potentially mediated by inhibiting mTOR/p70S6K signaling and enhancing autophagy activity.
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http://dx.doi.org/10.1016/j.ejphar.2020.173318DOI Listing
September 2020

The compensatory phenomenon of the functional connectome related to pathological biomarkers in individuals with subjective cognitive decline.

Transl Neurodegener 2020 05 27;9(1):21. Epub 2020 May 27.

Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, 321 Zhongshan Road, Nanjing, Jiangsu, 210008, P. R. China.

Background: Subjective cognitive decline (SCD) is a preclinical stage along the Alzheimer's disease (AD) continuum. However, little is known about the aberrant patterns of connectivity and topological alterations of the brain functional connectome and their diagnostic value in SCD.

Methods: Resting-state functional magnetic resonance imaging and graph theory analyses were used to investigate the alterations of the functional connectome in 66 SCD individuals and 64 healthy controls (HC). Pearson correlation analysis was computed to assess the relationships among network metrics, neuropsychological performance and pathological biomarkers. Finally, we used the multiple kernel learning-support vector machine (MKL-SVM) to differentiate the SCD and HC individuals.

Results: SCD individuals showed higher nodal topological properties (including nodal strength, nodal global efficiency and nodal local efficiency) associated with amyloid-β levels and memory function than the HC, and these regions were mainly located in the default mode network (DMN). Moreover, increased local and medium-range connectivity mainly between the bilateral parahippocampal gyrus (PHG) and other DMN-related regions was found in SCD individuals compared with HC individuals. These aberrant functional network measures exhibited good classification performance in the differentiation of SCD individuals from HC individuals at an accuracy up to 79.23%.

Conclusion: The findings of this study provide insight into the compensatory mechanism of the functional connectome underlying SCD. The proposed classification method highlights the potential of connectome-based metrics for the identification of the preclinical stage of AD.
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http://dx.doi.org/10.1186/s40035-020-00201-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254770PMC
May 2020

Effects of Angiotensin-Converting Enzyme Inhibitors on Arterial Stiffness: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Cardiovasc Ther 2020 25;2020:7056184. Epub 2020 Feb 25.

Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, China.

To determine the effects of ACEIs on arterial stiffness, a meta-analysis of randomized controlled trials was conducted. Relevant articles that investigated the effects of ACEIs on arterial stiffness from PubMed, Embase, and the Cochrane library from inception to September 2018 were systematically retrieved. The investigated outcomes included brachial-ankle pulse wave velocity (ba-PWV) and carotid-femoral PWV (cf-PWV) by using weighted mean differences (WMDs) and 95% confidence intervals (CIs) with the random-effects model. A total of 17 RCTs including 1,458 individuals were included. The summary results indicated no significant differences between ACEIs and control for ba-PWV and cf-PWV. Also, no significant differences between ACEI and control for ba-PWV and cf-PWV were observed in hypertensive patients, while the therapeutic effects of ACEI versus placebo showed statistically significant difference. Moreover, subgroup analysis indicated that the levels of ba-PWV were significantly associated if the study was conducted in Western countries, mean age <60.0 years, percentage male ≥60.0%, compared with ARBs, baseline PWV <10.0, and high-quality study. Furthermore, the significant levels of cf-PWV in patients who received ACEIs were observed when percentage male was ≥60.0% and the studies were of high-quality. Finally, no significant differences were observed between ACEIs and other antihypertensive drugs regarding the changes of systolic blood pressure (SBP) and diastolic blood pressure (DBP). The overall analysis suggested no significant differences between ACEIs and other antihypertensive drugs for ba-PWV and cf-PWV levels, whereas ACEIs versus placebo showed lower levels of ba-PWV and cf-PWV.
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http://dx.doi.org/10.1155/2020/7056184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068147PMC
June 2020

IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.

Theranostics 2020 16;10(5):2358-2373. Epub 2020 Jan 16.

Guangdong Key Laboratory of Genome Instability and Human Disease Prevention, Department of Biochemistry and Molecular Biology, Shenzhen University School of Medicine, Shenzhen 518055, China.

Invadopodia formation is a key driver of cancer metastasis. The noncanonical IkB-related kinase IKKε has been implicated in cancer metastasis, but its roles in invadopodia formation and colorectal cancer (CRC) metastasis are unclear. : Immunofluorescence, gelatin-degradation assay, wound healing assay and transwell invasion assay were used to determine the influence of IKKε over-expression, knockdown and pharmacological inhibition on invadopodia formation and the migratory and invasive capacity of CRC cells . Effects of IKKε knockdown or pharmacological inhibition on CRC metastasis were examined in mice. Immunohistochemistry staining was used to detect expression levels of IKKε in CRC patient tissues, and its association with prognosis in CRC patients was also analyzed. Immunoprecipitation, western blotting and kinase assay were constructed to investigate the molecular mechanisms. : IKKε co-localizes with F-actin and the invadopodia marker Tks5 at the gelatin-degrading sites of CRC cells. Genetic over-expression/knockdown or pharmacological inhibition of IKKε altered invadopodia formation and the migratory and invasive capacity of CRC cells . , knockdown or pharmacological inhibition of IKKε significantly suppressed metastasis of CRC cells in mice. IKKε knockdown also inhibited invadopodia formation . Clinical investigation of tumor specimens from 191 patients with CRC revealed that high IKKε expression correlates with metastasis and poor prognosis of CRC. Mechanistically, IKKε directly binds to and phosphorylates kindlin-2 at serine 159; this effect mediates the IKKε-induced invadopodia formation and promotion of CRC metastasis. : We identify IKKε as a novel regulator of invadopodia formation and a unique mechanism by which IKKε promotes the metastasis of CRC. Our study suggests that IKKε is a potential target to suppress CRC metastasis.
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http://dx.doi.org/10.7150/thno.40397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019159PMC
February 2021

White Matter Microstructural Damage as an Early Sign of Subjective Cognitive Decline.

Front Aging Neurosci 2019 28;11:378. Epub 2020 Jan 28.

Department of Neurology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

: Subjective cognitive decline (SCD) is considered a preclinical state of Alzheimer's disease (AD) and may represent a more advanced preclinical status than amnestic mild cognitive impairment (aMCI). Our aim was to explore changes in the white matter (WM) microstructure and their correlation with cognitive function in these AD-spectrum patients. : Diffusion tensor images from 43 individuals with normal cognition (NC), 38 SCD patients, and 36 aMCI patients were compared using an atlas-based segmentation strategy. The correlation between diffusion parameters and cognitive function was further analyzed. : The anatomical pattern of WM impairment was generally similar between SCD and aMCI patients. However, aMCI patients showed significantly lower fractional anisotropy (i.e., corpus callosum forceps major and forceps minor) and increased mean diffusivity [i.e., bilateral anterior thalamic radiation (ATR), left corticospinal tract (CST), forceps minor, left cingulum (cingulate gyrus), left cingulum hippocampus, and left inferior fronto-occipital fasciculus (IFO)] in some tracts than did SCD subjects, indicating a disruption in WM microstructural integrity in the aMCI. Individuals with microstructural disruption in forceps minor, left cingulum (cingulate gyrus), and left cingulum hippocampus tracts performed worse in general cognition and memory function tests, as indicated by line regression analysis. : SCD individuals had extensive WM microstructural damage in a pattern similar to that seen in aMCI, although presenting a cognitive performance comparable with that of cognitively healthy individuals. Our results suggest that WM integrity might precede objectively measurable memory decline and may be a potential early biomarker for AD.
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http://dx.doi.org/10.3389/fnagi.2019.00378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997435PMC
January 2020

The assembly and antitumor activity of lycium barbarum polysaccharide-platinum-based conjugates.

J Inorg Biochem 2020 04 24;205:111001. Epub 2020 Jan 24.

Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224051, People's Republic of China. Electronic address:

In this work, the new polysaccharide-platinum conjugates of 5-aminosalicylic acid modified lycium barbarum polysaccharide linking platinum compounds were designed in order to construct an anticancer metal drug delivery system. The multiple analysis methods were used to describe the chemical structure and physical properties of the polysaccharide-metal conjugates. The results showed that 5-aminosalicylic acid successfully acted as linker which was covalently bound between polysaccharide and platinum compound. The morphology and rheological properties of polysaccharide have been changed by the formation of conjugates, which exhibited certain inhibition specificity to A549 (human lung cancer cell line). The agarose gel electrophoresis and fluorescence microscopy results demonstrated that such conjugates promoted the unwinding of DNA and could significantly damage the nucleus of A549 cells. Cell cycle analyzing the Pt complex of conjugates could cause intracellular DNA damage and induced G2 phase arrest. So, polysaccharide-platinum conjugates might find a range of applications, for example in metal anticancer drug delivery.
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http://dx.doi.org/10.1016/j.jinorgbio.2020.111001DOI Listing
April 2020

Corrigendum to: "Central metal-derived co-assembly of biomimetic GdTPP/ZnTPP porphyrin nanocomposites for enhanced dual-modal imaging-guided photodynamic therapy" [Biomaterials 229 (2020) 119576].

Biomaterials 2020 Mar 20;235:119758. Epub 2020 Jan 20.

International Joint Center for Biomedical Innovation, School of Life Sciences Henan University, Kaifeng, 475004, China; Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, Macquarie University, Sydney, NSW, 2109, Australia. Electronic address:

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http://dx.doi.org/10.1016/j.biomaterials.2020.119758DOI Listing
March 2020

The efficacy of gray matter atrophy and cognitive assessment in differentiation of aMCI and naMCI.

Appl Neuropsychol Adult 2020 Jan 16:1-7. Epub 2020 Jan 16.

Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

Mild cognitive impairment (MCI) is a heterogeneous entity that can be categorized into related but different subtypes. In this study, we analyzed the gray matter structural changes of amnestic MCI (aMCI) and non-amnestic MCI (naMCI), and how it resulted in diverse cognitive impairment. Altogether 77 individuals were recruited, including 28 cognitively normal controls (NC), 25 naMCI subjects, and 24 aMCI subjects. All participants underwent a 3.0 T magnetic resonance (MR) scan and a detailed neuropsychological examination. Cortical thickness and subcortical nuclei volume were extracted by Freesurfer software and compared among groups. The areas with significant differences were further analyzed by general linear regression to identify the risk factors of each cognitive impairment subtypes. Significant differences were observed in bilateral hippocampi, amygdala, thalamus, accumbens, left transverse temporal gyrus and left precuneus among groups. AMCI and naMCI were significantly different in the right hippocampus, bilateral amygdala, left precuneus, and left transverse temporal gyrus. Linear regression analysis revealed that the atrophy of left precuneus was a risk factor of memory, executive function (EF) and visuospatial impairment ( < 0.001). The atrophy of left amygdala, right accumbens and left thalamus were risk factors of memory, EF and language impairment respectively ( < 0.05). These findings confirmed that different gray matter structural changes could lead to specific neuropsychological features in MCI subtypes. Thorough understanding of MCI subtypes and the underlying pathology would be beneficial for precise diagnosis and intervention.
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http://dx.doi.org/10.1080/23279095.2019.1710509DOI Listing
January 2020

Engineering Tropane Alkaloid Production Based on Metabolic Characterization of Ornithine Decarboxylase in .

ACS Synth Biol 2020 02 22;9(2):437-448. Epub 2020 Jan 22.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences , Southwest University , Chongqing 400715 , China.

Ornithine decarboxylase (ODC) plays an important role in various biological processes; however, its role in plant secondary metabolism, especially in the biosynthesis of tropane alkaloids (TAs) such as pharmaceutical hyoscyamine, anisodamine, and scopolamine, remains largely unknown. In this study, we characterized the physiological and metabolic functions of the gene of () and determined its role in TA production using metabolic engineering approaches. Feeding assays with enzyme inhibitors indicated that ODC, rather than arginine decarboxylase (ADC), plays a major role in TA biosynthesis. Tissue-specific expression analysis and β-glucuronidase (GUS) staining assays showed that was highly expressed in secondary roots, especially in the cylinder tissue. Enzymatic assays indicated that AbODC was able to convert ornithine to putrescine, with the highest activity at pH 8.0 and 30 °C. Additionally, AbODC showed higher catalytic efficiency than other plant ODCs, as evident from the , , and values of AbODC using ornithine as the substrate. In root cultures, suppression of greatly reduced the production of putrescine, -methylputrescine, and TAs, whereas overexpression of significantly increased the biosynthesis of putrescine, -methylputrescine, hyoscyamine, and anisodamine. Moreover, transgenic plants overexpressing showed a significantly higher production of hyoscyamine and anisodamine compared with control plants. These findings indicate that AbODC plays a key role in TA biosynthesis and therefore is a valuable candidate for increasing TA production in .
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http://dx.doi.org/10.1021/acssynbio.9b00461DOI Listing
February 2020

Steroidogenic acute regulatory protein/aldosterone synthase mediates angiotensin II-induced cardiac fibrosis and hypertrophy.

Mol Biol Rep 2020 Feb 9;47(2):1207-1222. Epub 2019 Dec 9.

Department of Basic Biomedical Sciences, Mercer University School of Medicine, Savannah, GA, USA.

Aldosterone produced in adrenal glands by angiotensin II (Ang II) is known to elicit myocardial fibrosis and hypertrophy. This study was designed to test the hypothesis that Ang II causes cardiac morphological changes through the steroidogenic acute regulatory protein (StAR)/aldosterone synthase (AS)-dependent aldosterone synthesis primarily initiated in the heart. Sprague-Dawley rats were randomized to following groups: Ang II infusion for a 4-week period, treatment with telmisartan, spironolactone or adrenalectomy during Ang II infusion. Sham-operated rats served as control. Relative to Sham rats, Ang II infusion significantly increased the protein levels of AT1 receptor, StAR, AS and their tissue expression in the adrenal glands and heart. In coincidence with reduced aldosterone level in the heart, telmisartan, an AT1 receptor blocker, significantly down-regulated the protein level and expression of StAR and AS. Ang II induced changes in the expression of AT1/StAR/AS were not altered by an aldosterone receptor antagonist spironolactone. Furthermore, Ang II augmented migration of macrophages, protein level of TGFβ1, phosphorylation of Smad2/3 and proliferation of myofibroblasts, accompanied by enhanced perivascular/interstitial collagen deposition and cardiomyocyte hypertrophy, which all were significantly abrogated by telmisartan or spironolactone. However, adrenalectomy did not fully suppress Ang II-induced cell migration/proliferation and fibrosis/hypertrophy, indicating a role of aldosterone synthesized within the heart in pathogenesis of Ang II induced injury. These results indicate that myocardial fibrosis and hypertrophy stimulated by Ang II is associated with tissue-specific activation of aldosterone synthesis, primarily mediated by AT1/StAR/AS signaling pathways.
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http://dx.doi.org/10.1007/s11033-019-05222-7DOI Listing
February 2020

Conservation of glucagon like peptide-1 level with liraglutide and linagilptin protects the kidney against angiotensin II-induced tissue fibrosis in rats.

Eur J Pharmacol 2020 Jan 4;867:172844. Epub 2019 Dec 4.

Cardiovascular Research Laboratory, Mercer University School Medicine, Savannah, GA, USA. Electronic address:

This study tested the hypothesis that the enhancement of glucagon-like peptide-1 (GLP-1) level through either exogenous supply of GLP-1 agonist, liraglutide or prevention of endogenous GLP-1 degradation with dipeptidyl peptidease-4 inhibitor, lingaliptin ameliorates angiotensin II (Ang II)-induced renal fibrosis. Sprague-Dawley rats were randomly divided into four groups: 0.9% saline or Ang II (500 ng/kg/min) was infused with osmotic minipumps for 4 weeks, defined as sham and Ang II groups. In drug treated groups, liraglutide (0.3 mg/kg) was injected subcutaneously twice daily or linagliptin (8 mg/kg) was administered daily via oral gavage during Ang II infusion. Compared with Ang II stimulation, liraglutide or linagliptin comparatively down-regulated the protein level of the AT receptor, and up-regulated the AT receptor, as identified by a reduced AT/AT ratio (all p < 0.05), consistent with less locally-expressed AT receptor and enhanced AT receptor in the glomerular capillaries and proximal tubules of the renal cortex. Furthermore, both drugs significantly increased the expression of GLP-1 receptor and attenuated the protein levels of TLR4, NOX4 and IL-6. The populations of macrophages and α-SMA expressing myofibroblasts decreased with treatment of liraglutide and linagliptin, in coincidence with the reduced expression of phosphor-Smad2/3, Smad4, TGFβ1, and up-regulated Smad7. Along with these modulations, renal morphology was preserved and synthesis of fibronectin/collagen I was down-regulated, as identified by small collagen-rich area in the renal cortex. These results suggest that the preservation of GLP-1 level using liraglutide or linagliptin might be considered as an add-on therapeutic option for inhibiting Ang II induced renal fibrosis and failure.
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http://dx.doi.org/10.1016/j.ejphar.2019.172844DOI Listing
January 2020

Triazine Poly(carboxylic acid) Metal-Organic Frameworks and the Fluorescent Response with Lead Oxygen Clusters: [Pb(COO)X] by Halogen Tuning (X = Cl, Br, or I).

Inorg Chem 2019 Dec 14;58(23):15898-15908. Epub 2019 Nov 14.

College of Chemistry and Chemical Engineering , Liaoning Normal University , Huanghe Road 850# , Dalian 116029 , P. R. China.

A series of novel metal-organic frameworks were synthesized by using semirigid ligand 1,1',1″-(1,3,5-triazine-2,4,6-triyl)tripiperidine-4-carboxylic acid (HTTPCA) and lead halide (Cl, Br, or I). The three complexes were characterized by elemental analysis, infrared spectroscopy, ultraviolet-visible spectroscopy, powder X-ray diffraction analysis, and thermogravimetric analysis. X-ray single-crystal diffraction analysis demonstrated that all three complexes were three-dimensional inorganic-organic framework structures with Pb-X (X = Cl, Br, or I). However, slight differences in the chemical environment were the focus of the coordinated halogen atoms and the different compositions of metal oxygen clusters: [Pb(COO)Cl], [Pb(COO)Br], and [Pb(COO)I]. Because of the fluorescence of the organic ligand, the three complexes showed similar photoluminescence properties at room temperature, but the intensity of emissions decreased gradually with an increase in the atomic radius of coordinated halogen atoms. Interestingly, in the fluorescence response tests, complexes and displayed an optical signal of fluorescence "turn-on" while complex showed an optical signal of fluorescence "turn-off". Here we aim to provide a possible mechanism to explain these unique and contradictory luminescence results.
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http://dx.doi.org/10.1021/acs.inorgchem.9b02365DOI Listing
December 2019

Central metal-derived co-assembly of biomimetic GdTPP/ZnTPP porphyrin nanocomposites for enhanced dual-modal imaging-guided photodynamic therapy.

Biomaterials 2020 01 24;229:119576. Epub 2019 Oct 24.

International Joint Center for Biomedical Innovation, School of Life Sciences Henan University, Kaifeng, 475004, China; Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, Macquarie University, Sydney, NSW, 2109, Australia. Electronic address:

Dual-modal imaging guided photodynamic therapy (PDT) of multifunctional nanocomposites holds great promise for precision tumor theranostics. However, poor heterogeneous interfacial compatibility between functional components, low hydrophilicity and complicated preparation of nanocomposites remain major obstacles for further bioapplication. Herein, a facile central metal-derived co-assembly strategy is developed to effectively integrate gadolinium porphyrin (GdTPP) contrast agent and Zinc porphyrin (ZnTPP) photosensitizer into a homogeneous GdTPP/ZnTPP nanocomposites (GZNs). GZNs possesses the following advantages: (1) Greatly improved interfacial compatibility facilitated by incorporating two metalporphyrins with same group (phenyl-) and different central metal atoms (Zn and Gd) leading to higher yield (4.7-5 fold) than either monocomponent nanoparticles. (2) Poor dispersity of GdTPP nanoparticles is greatly improved after integrating with ZnTPP blocks. (3) The GZNs inherit excellent fluorescence imaging, high relaxation rate (8.18 mM s) and singlet oxygen production from two raw metalporphyrins. After camouflaging with homotypic cancer cell membrane for immunologic escape, the HeLa membrane coated GZNs (mGZNs) show enhanced in vivo MR/FL imaging guided anti-tumor targeting efficiency of 80.6% for HeLa cells. Our new strategy using central metal-derived co-assembly of homogeneous building blocks greatly improves interfacial compatibility to achieve combined functions for visualized cancer theranostics.
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http://dx.doi.org/10.1016/j.biomaterials.2019.119576DOI Listing
January 2020

Liraglutide attenuates cardiac remodeling and improves heart function after abdominal aortic constriction through blocking angiotensin II type 1 receptor in rats.

Drug Des Devel Ther 2019 6;13:2745-2757. Epub 2019 Aug 6.

Department of Physiology, Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.

Objective: Angiotensin II (Ang II) is known to contribute to the pathogenesis of heart failure by eliciting cardiac remodeling and dysfunction. The glucagon-like peptide-1 (GLP-1) has been shown to exert cardioprotective effects in animals and patients. This study investigates whether GLP-1 receptor agonist liraglutide inhibits abdominal aortic constriction (AAC)-induced cardiac fibrosis and dysfunction through blocking Ang II type 1 receptor (AT1R) signaling.

Methods: Sprague-Dawley rats were subjected to sham operation and abdominal aortic banding procedure for 16 weeks. In treated rats, liraglutide (0.3 mg/kg) was subcutaneously injected twice daily or telmisartan (10 mg/kg/day), the AT1R blocker, was administered by gastric gavage.

Results: Relative to the animals with AAC, liraglutide reduced protein level of the AT1R and upregulated the AT2R, as evidenced by reduced ratio of AT1R/AT2R (0.59±0.04 vs. 0.91±0.06, <0.05). Furthermore, the expression of angiotensin converting enzyme 2 was upregulated, tissue levels of malondialdehyde and B-type natriuretic peptide were reduced, and superoxide dismutase activity was increased. Along with a reduction in HW/BW ratio, cardiomyocyte hypertrophy was inhibited. In coincidence with these changes, liraglutide significantly decreased the populations of macrophages and myofibroblasts in the myocardium, which were accompanied by reduced protein levels of transforming growth factor beta1, Smad2/3/4, and upregulated smad7. The synthesis of collagen I and III was inhibited and collagen-rich fibrosis was attenuated. Consistent with these findings, cardiac systolic function was preserved, as shown by increased left ventricular systolic pressure (110±5 vs. 99±2 mmHg, <0.05), ejection fraction (83%±2% vs. 69%±4%, <0.05) and fraction shortening (49%±2% vs. 35%±3%, <0.05). Treatment with telmisartan provided a comparable level of protection as compared with liraglutide in all the parameters measured.

Conclusion: Taken together, liraglutide ameliorates cardiac fibrosis and dysfunction, potentially via suppressing the AT1R-mediated events. These data indicate that liraglutide might be selected as an add-on drug to prevent the progression of heart failure.
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http://dx.doi.org/10.2147/DDDT.S213910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690048PMC
April 2020

Enhanced Regional Homogeneity and Functional Connectivity in Subjects With White Matter Hyperintensities and Cognitive Impairment.

Front Neurosci 2019 3;13:695. Epub 2019 Jul 3.

Department of Neurology, Affiliated Drum Tower Hospital, Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, China.

Objective: White matter hyperintensities (WMH) is an important cause of vascular cognitive impairment (CI). However, a considerable portion of individuals with WMH do not develop CI. The present study aimed to investigate distinctive regional brain activity and connectivity patterns in WMH subjects with and without CI, who displayed comparable WMH burden.

Methods: Fourteen WMH subjects with CI, 16 WMH subjects without CI and 37 healthy subjects underwent multimodal MRI scans and neuropsychological tests. All WMH subjects displayed Fazekas grade 2 of WMH. Regional Homogeneity (ReHo) and functional connectivity (FC) patterns were identified based on resting-state functional MRI data.

Results: No significant differences in WMH volume, the number of WMH lesions and brain volume were shown between the 2 WMH groups. In contrast, the WMH with CI group showed higher ReHo in bilateral superior parietal gyrus (SPG)/superior occipital gyrus (SOG) than the WMH without CI group. Compared with the WMH without CI group, the WMH with CI group also displayed higher FC of the left SPG/SOG with frontal regions, and higher FC of the right SPG/SOG with parietal regions. Furthermore, higher FC of the left SPG/SOG with frontal regions were significantly associated with less worse executive dysfunction in WMH with CI subjects, suggesting a compensatory effect.

Conclusion: Higher local coherence of activities in the SPG/SOG and higher connectivity of the SPG/SOG with parietal and frontal regions are related to CI in WMH subjects. The findings provide novel insights into functional alterations underlying the cognitive variety in WMH subjects.
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http://dx.doi.org/10.3389/fnins.2019.00695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617843PMC
July 2019

The potential role of testosterone in hypertension and target organ damage in hypertensive postmenopausal women.

Clin Interv Aging 2019 29;14:743-752. Epub 2019 Apr 29.

Department of Cardiology, Lanzhou University Second Hospital, Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.

The aim of this study was to confirm the potential role of testosterone in hypertension and target organ damage (TOD) in hypertensive postmenopausal women. A matched group study was conducted. One hundred sixty-one hypertensive postmenopausal women between 45 and 65 years of age were enrolled as group 1. Another 161 age-matched hypertensive men were enrolled as group 2. Ambulatory blood pressure monitoring, echocardiographic imaging, vascular function, sex hormones and clinical characteristics were evaluated. Quantitative data were analyzed using independent Student's -test and multiple regression analysis. The mean and load level of blood pressure were lower in women than in men (<0.05), except for the mean level and load of the nocturnal systolic blood pressure (SBP) (123.77±15.72 mmHg vs 126.35±15.64 mmHg, and 50.43±30.31% vs 55.35±28.51%, >0.05). However, the carotid-femoral pulse wave velocity (cf-PWV) in women was higher than that in men (9.68±2.23 m/s vs 8.03±2.82 m/s, <0.05). The ratio of the early diastolic mitral peak flow velocity to early diastolic mitral annular velocity (E/Em) was obviously impaired (13.06±3.53 vs 12.05±3.68, <0.05) in women. Furthermore, in women, a positive correlation was found between testosterone and cf-PWV (γ=0.157, =0.046), and Cf-PWV was positively related to the mean level of nighttime SBP (γ=0.210, =0.008). Moreover, nocturnal SBP was a risk factor for E/Em (γ=0.156, =0.048, <0.05). Testosterone may play a role in the correlation between hypertension and TOD in hypertensive postmenopausal women. This research study was registered under the ClinicalTrials.gov PRS Website (NCT03451747).
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http://dx.doi.org/10.2147/CIA.S195498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501555PMC
August 2019

Does virtue lead to status? Testing the moral virtue theory of status attainment.

J Pers Soc Psychol 2020 Mar 20;118(3):501-531. Epub 2019 May 20.

School of Management, Zhejiang University.

[Correction Notice: An Erratum for this article was reported online in on Oct 24 2019 (see record 2019-63656-001). In the article "Does Virtue Lead to Status? Testing the Moral Virtue Theory of Status Attainment" by Feng Bai, Grace Ching Chi Ho, and Jin Yan, funding acknowledgments were omitted from the author note. The second paragraph of the author note should read as follows: This research was funded by an internal start-up grant (1-ZE74) awarded to Feng Bai from the Hong Kong Polytechnic University and a grant (NSFC 71572175) awarded to Jin Yan from the National Natural Science Foundation of China.] The authors perform one of the first empirical tests of the moral virtue theory of status attainment (MVT), a conceptual framework for showing that morality leads to status. Studies 1a to 1d are devoted to developing and validating a 15-item status attainment scale (SAS) to measure how virtue leads to admiration (virtue-admiration), how dominance leads to fear (dominance-fear), and how competence leads to respect (competence-respect). Studies 2a and 2b are an exploration of the nomological network and discriminant validity to show that peer-reported virtue-admiration is positively related to moral character and perceptions such as perceived warmth and unrelated to amoral constructs such as neuroticism. In addition, virtue-admiration mediates the positive effect of several self-reported moral character traits, such as moral identity-internalization, on status conferral. Study 3 supports the external validity of the virtue route to status in a sample of fulltime managers from China. In Study 4, a preregistered experiment, virtue evokes superior status while selfishness evokes inferior status. Perceivers who are high in moral character show stronger perceptions of superior status. Finally, Study 5, another preregistered experiment, shows that virtue leads to higher status through inducing virtue-admiration rather than competence-respect, even for incompetent actors. The findings provide initial support for MVT arguing that virtue is a distinct, third route to status. (PsycINFO Database Record (c) 2020 APA, all rights reserved).
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http://dx.doi.org/10.1037/pspi0000192DOI Listing
March 2020

Pressure Induced Nanoparticle Phase Behavior, Property, and Applications.

Chem Rev 2019 06 6;119(12):7673-7717. Epub 2019 May 6.

Sandia National Laboratories, Albuquerque, New Mexico 87185, United States.

Nanoparticle (NP) high pressure behavior has been extensively studied over the years. In this review, we summarize recent progress on the studies of pressure induced NP phase behavior, property, and applications. This review starts with a brief overview of high pressure characterization techniques, coupled with synchrotron X-ray scattering, Raman, fluorescence, and absorption. Then, we survey the pressure induced phase transition of NP atomic crystal structure including size dependent phase transition, amorphization, and threshold pressures using several typical NP material systems as examples. Next, we discuss the pressure induced phase transition of NP mesoscale structures including topics on pressure induced interparticle separation distance, NP coupling, and NP coalescence. Pressure induced new properties and applications in different NP systems are highlighted. Finally, outlooks with future directions are discussed.
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http://dx.doi.org/10.1021/acs.chemrev.9b00023DOI Listing
June 2019

Technical note: Evaluation of interval between measurements and calculation method for the quantification of enteric methane emissions measured by respiration chamber.

J Dairy Sci 2019 Jul 2;102(7):6242-6247. Epub 2019 May 2.

CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, China.

Respiration chambers share one analyzer working in parallel, and methane (CH) concentrations have to be measured at certain intervals. The maximum and minimum values in the kinetics of CH emissions can be missed during the interval between measurements, which may influence the quantification of CH emissions. Chambers must be opened for morning feeding and cleaning, which causes a loss of CH data. Calculation methods are needed to estimate the lost CH emission data, which may influence the estimated amount of daily CH emissions. In this study, we measured the CH emissions of 10 growing Chinese Holstein dairy heifers in respiration chambers. Methane concentrations were measured every 0.5 min to obtain the 23-h kinetics of CH emissions, which were further selected at different intervals between measurements (i.e., 5, 30, 60, 120, 180, and 240 min) to evaluate the effects of interval on quantification of CH emissions. The missing 1-h kinetics of CH emissions before feeding were not measured, and 2 calculation methods were used to estimate the missing 1-h kinetics of CH emissions: mean value of measuring period (the mean method) and the nearest value of measurement just before chamber opening (the nearest method). The results showed that the rates of CH emission from 10 heifers varied from 4.56 to 11.42 g/h. The increment of intervals decreased maximum rate of CH emission and increased minimum rate of CH emission. Interval caused less than 5% of the difference in measuring CH emissions. Although the mean method had greater estimated daily CH emission than the nearest method, the difference was within 3%. The interval between measurements (≤3 h) and calculation method had little influence on enteric CH emission measurements.
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http://dx.doi.org/10.3168/jds.2019-16245DOI Listing
July 2019