Felix Frolow

Felix Frolow

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Felix Frolow

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Pore-modulating toxins exploit inherent slow inactivation to block K channels.

Proc Natl Acad Sci U S A 2019 09 23;116(37):18700-18709. Epub 2019 Aug 23.

Department of Biomolecular Sciences, Weizmann Institute of Science, 76100 Rehovot, Israel;

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http://dx.doi.org/10.1073/pnas.1908903116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744907PMC
September 2019

Directed Evolution of β-Glucosidase A Towards Enhanced Thermostability.

Int J Mol Sci 2019 Sep 23;20(19). Epub 2019 Sep 23.

Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot 7610001, Israel.

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http://dx.doi.org/10.3390/ijms20194701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801902PMC
September 2019

Reassembly and co-crystallization of a family 9 processive endoglucanase from its component parts: structural and functional significance of the intermodular linker.

PeerJ 2015 15;3:e1126. Epub 2015 Sep 15.

Department of Molecular Microbiology and Biotechnology, The Daniella Rich Institute for Structural Biology, Tel Aviv University , Ramat Aviv , Israel.

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http://dx.doi.org/10.7717/peerj.1126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579020PMC
September 2015

Standalone cohesin as a molecular shuttle in cellulosome assembly.

FEBS Lett 2015 Jun 17;589(14):1569-76. Epub 2015 Apr 17.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Israel; The Daniella Rich Institute for Structural Biology, Tel Aviv University, 69978, Israel.

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http://dx.doi.org/10.1016/j.febslet.2015.04.013DOI Listing
June 2015

Crystal structure of the human mitochondrial chaperonin symmetrical football complex.

Proc Natl Acad Sci U S A 2015 May 27;112(19):6044-9. Epub 2015 Apr 27.

Departments of Biochemistry and Molecular Biology and The Daniella Rich Institute for Structural Biology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

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http://dx.doi.org/10.1073/pnas.1411718112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434751PMC
May 2015

Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson-Crick base pairing.

Nat Nanotechnol 2015 Apr 16;10(4):353-60. Epub 2015 Mar 16.

1] Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel [2] Department of Materials Science and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, Ramat Aviv 69978, Israel.

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http://dx.doi.org/10.1038/nnano.2015.27DOI Listing
April 2015

Protein products obtained by site-preferred partial crosslinking in protein crystals and "liberated" by redissolution.

Biotechnol Bioeng 2014 Jul 25;111(7):1296-303. Epub 2014 Jan 25.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978, Tel-Aviv, Israel.

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http://dx.doi.org/10.1002/bit.25186DOI Listing
July 2014

Structural characterization of a novel autonomous cohesin from Ruminococcus flavefaciens.

Acta Crystallogr F Struct Biol Commun 2014 Apr 25;70(Pt 4):450-6. Epub 2014 Mar 25.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1107/S2053230X14004051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976060PMC
April 2014

Fine-structural variance of family 3 carbohydrate-binding modules as extracellular biomass-sensing components of Clostridium thermocellum anti-σI factors.

Acta Crystallogr D Biol Crystallogr 2014 Feb 31;70(Pt 2):522-34. Epub 2014 Jan 31.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, Israel.

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http://dx.doi.org/10.1107/S139900471302926XDOI Listing
February 2014

Crystallization and structure determination of a symmetrical 'football' complex of the mammalian mitochondrial Hsp60-Hsp10 chaperonins.

Acta Crystallogr F Struct Biol Commun 2014 Jan 24;70(Pt 1):116-9. Epub 2013 Dec 24.

The Daniella Rich Institute for Structural Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel.

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http://dx.doi.org/10.1107/S2053230X1303389XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943094PMC
January 2014

Structure of a family 3a carbohydrate-binding module from the cellulosomal scaffoldin CipA of Clostridium thermocellum with flanking linkers: implications for cellulosome structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Jul 27;69(Pt 7):733-7. Epub 2013 Jun 27.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, Israel.

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http://dx.doi.org/10.1107/S174430911301614XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702315PMC
July 2013

A triclinic crystal structure of the carboxy-terminal domain of HIV-1 capsid protein with four molecules in the asymmetric unit reveals a novel packing interface.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Jun 23;69(Pt 6):602-6. Epub 2013 May 23.

Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, Israel.

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http://dx.doi.org/10.1107/S1744309113011871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668575PMC
June 2013

A simple method for determining specificity of carbohydrate-binding modules for purified and crude insoluble polysaccharide substrates.

Methods Mol Biol 2012 ;908:101-7

Department of Molecular Microbiology and Biotechnology, The Daniella Rich Institute for Structural Biology, Tel Aviv University, Ramat Aviv, Israel.

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http://dx.doi.org/10.1007/978-1-61779-956-3_10DOI Listing
December 2012

Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides.

Methods Enzymol 2012 ;510:247-59

Department of Molecular Microbiology and Biotechnology, The Daniella Rich Institute for Structural Biology, Tel Aviv University, Ramat Aviv, Israel.

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http://dx.doi.org/10.1016/B978-0-12-415931-0.00013-6DOI Listing
September 2012

A single mutation reforms the binding activity of an adhesion-deficient family 3 carbohydrate-binding module.

Acta Crystallogr D Biol Crystallogr 2012 Jul 15;68(Pt 7):819-28. Epub 2012 Jun 15.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1107/S0907444912013133DOI Listing
July 2012

Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Jan 24;68(Pt 1):8-13. Epub 2011 Dec 24.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, Israel.

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http://dx.doi.org/10.1107/S174430911104807XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253825PMC
January 2012

Scaffoldin-borne family 3b carbohydrate-binding module from the cellulosome of Bacteroides cellulosolvens: structural diversity and significance of calcium for carbohydrate binding.

Acta Crystallogr D Biol Crystallogr 2011 Jun 12;67(Pt 6):506-15. Epub 2011 May 12.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1107/S0907444911011322DOI Listing
June 2011

Re-structuring protein crystals porosity for biotemplating by chemical modification of lysine residues.

Biotechnol Bioeng 2011 Jan;108(1):1-11

Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.

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http://doi.wiley.com/10.1002/bit.22921
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http://dx.doi.org/10.1002/bit.22921DOI Listing
January 2011

Noncellulosomal cohesin from the hyperthermophilic archaeon Archaeoglobus fulgidus.

Proteins 2011 Jan 15;79(1):50-60. Epub 2010 Oct 15.

Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.

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http://dx.doi.org/10.1002/prot.22857DOI Listing
January 2011

Modular arrangement of a cellulosomal scaffoldin subunit revealed from the crystal structure of a cohesin dyad.

J Mol Biol 2010 Jun 13;399(2):294-305. Epub 2010 Apr 13.

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://linkinghub.elsevier.com/retrieve/pii/S002228361000364
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http://dx.doi.org/10.1016/j.jmb.2010.04.013DOI Listing
June 2010

Structure of a family 3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum: structural diversity and implications for carbohydrate binding.

Acta Crystallogr D Biol Crystallogr 2010 Jan 21;66(Pt 1):33-43. Epub 2009 Dec 21.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1107/S0907444909043030DOI Listing
January 2010

Cohesin diversity revealed by the crystal structure of the anchoring cohesin from Ruminococcus flavefaciens.

Proteins 2009 Nov;77(3):699-709

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1002/prot.22483DOI Listing
November 2009

Modification of protein crystal packing by systematic mutations of surface residues: implications on biotemplating and crystal porosity.

Biotechnol Bioeng 2009 Oct;104(3):444-57

Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

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http://doi.wiley.com/10.1002/bit.22427
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http://dx.doi.org/10.1002/bit.22427DOI Listing
October 2009

Intermodular linker flexibility revealed from crystal structures of adjacent cellulosomal cohesins of Acetivibrio cellulolyticus.

J Mol Biol 2009 Aug 6;391(1):86-97. Epub 2009 Jun 6.

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.

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http://dx.doi.org/10.1016/j.jmb.2009.06.006DOI Listing
August 2009

Crystallization and preliminary X-ray analysis of a cohesin-like module from AF2375 of the archaeon Archaeoglobus fulgidus.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Mar 26;65(Pt 3):275-8. Epub 2009 Feb 26.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Israel.

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http://scripts.iucr.org/cgi-bin/paper?S1744309109002887
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http://dx.doi.org/10.1107/S1744309109002887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650457PMC
March 2009

Physical association of the catalytic and helper modules of a family-9 glycoside hydrolase is essential for activity.

FEBS Lett 2009 Mar 12;583(5):879-84. Epub 2009 Feb 12.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel.

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http://doi.wiley.com/10.1016/j.febslet.2009.02.013
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http://dx.doi.org/10.1016/j.febslet.2009.02.013DOI Listing
March 2009

Structural basis of restoring sequence-specific DNA binding and transactivation to mutant p53 by suppressor mutations.

J Mol Biol 2009 Jan 30;385(1):249-65. Epub 2008 Oct 30.

Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.

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http://dx.doi.org/10.1016/j.jmb.2008.10.063DOI Listing
January 2009

Thiol-disulfide organization in alliin lyase (alliinase) from garlic (Allium sativum).

Protein Sci 2009 Jan;18(1):196-205

Department of Chemical Research Support, The Weizmann Institute of Science, 76100 Rehovot, Israel.

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http://dx.doi.org/10.1002/pro.10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708034PMC
January 2009

Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution.

Proteins 2008 Aug;72(2):711-9

Department of Organic Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1002/prot.21946DOI Listing
August 2008

Preliminary X-ray characterization of a novel type of anchoring cohesin from the cellulosome of Ruminococcus flavefaciens.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Feb 18;64(Pt 2):77-80. Epub 2008 Jan 18.

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1107/S1744309107067437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374186PMC
February 2008

Crystallization and preliminary X-ray analysis of Acetivibrio cellulolyticus cellulosomal type II cohesin module: two versions having different linker lengths.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Jan 20;64(Pt 1):58-61. Epub 2007 Dec 20.

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1107/S1744309107066821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373993PMC
January 2008

Crystallization and preliminary diffraction studies of CBM3b of cellobiohydrolase 9A from Clostridium thermocellum.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2007 Dec 21;63(Pt 12):1044-7. Epub 2007 Nov 21.

Department of Molecular Microbiology and Biotechnology, Tel Aviv University 69978, Israel.

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http://scripts.iucr.org/cgi-bin/paper?S1744309107054644
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http://dx.doi.org/10.1107/S1744309107054644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2344089PMC
December 2007

Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis.

Biotechnol Bioeng 2007 Oct;98(3):711-8

Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.

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http://doi.wiley.com/10.1002/bit.21459
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http://dx.doi.org/10.1002/bit.21459DOI Listing
October 2007

X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity.

J Mol Biol 2007 Feb 10;366(2):586-601. Epub 2006 Nov 10.

Department of Plant Sciences, George S. Wise Faculty of Life Sciences, and The Daniella Rich Institute for Structural Biology, Tel-Aviv University, Tel-Aviv 69978, Israel.

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http://dx.doi.org/10.1016/j.jmb.2006.10.085DOI Listing
February 2007

Two structures of alliinase from Alliium sativum L.: apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor.

J Mol Biol 2007 Feb 14;366(2):611-25. Epub 2006 Nov 14.

Department of Chemical Research Support, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1016/j.jmb.2006.11.041DOI Listing
February 2007

Monitoring the stability of crosslinked protein crystals biotemplates: a feasibility study.

Biotechnol Bioeng 2006 Aug;94(5):1005-11

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1002/bit.20935DOI Listing
August 2006

Structure of alcohol dehydrogenase from Entamoeba histolytica.

Acta Crystallogr D Biol Crystallogr 2006 May 19;62(Pt 5):541-7. Epub 2006 Apr 19.

Department of Chemical Research Support, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://scripts.iucr.org/cgi-bin/paper?S0907444906009292
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http://dx.doi.org/10.1107/S0907444906009292DOI Listing
May 2006

Crystal structure of yeast V-ATPase subunit C reveals its stator function.

EMBO Rep 2004 Dec;5(12):1148-52

Department of Biochemistry, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1038/sj.embor.7400294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1299189PMC
December 2004

Expression, crystallization and phasing of vacuolar H(+)-ATPase subunit C (Vma5p) of Saccharomyces cerevisiae.

Acta Crystallogr D Biol Crystallogr 2004 Oct 23;60(Pt 10):1906-9. Epub 2004 Sep 23.

Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1107/S0907444904019699DOI Listing
October 2004

Molecular basis of the high insecticidal potency of scorpion alpha-toxins.

J Biol Chem 2004 Jul 8;279(30):31679-86. Epub 2004 May 8.

Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel.

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http://dx.doi.org/10.1074/jbc.M402048200DOI Listing
July 2004

The ternary complex of Pseudomonas aeruginosa alcohol dehydrogenase with NADH and ethylene glycol.

Protein Sci 2004 Jun;13(6):1547-56

Department of Organic Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel.

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http://dx.doi.org/10.1110/ps.03531404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2279990PMC
June 2004

Evolution of photosystem I - from symmetry through pseudo-symmetry to asymmetry.

FEBS Lett 2004 Apr;564(3):274-80

Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1016/S0014-5793(04)00360-6DOI Listing
April 2004

Light-harvesting features revealed by the structure of plant photosystem I.

Photosynth Res 2004 ;81(3):239-50

Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel,

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http://dx.doi.org/10.1023/B:PRES.0000036881.23512.42DOI Listing
January 2004

Crystal structure of plant photosystem I.

Nature 2003 Dec;426(6967):630-5

Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

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http://dx.doi.org/10.1038/nature02200DOI Listing
December 2003

Crystallization and initial X-ray diffraction studies of higher plant photosystem I.

Acta Crystallogr D Biol Crystallogr 2003 Oct 19;59(Pt 10):1824-7. Epub 2003 Sep 19.

Department of Biological Chemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.

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http://dx.doi.org/10.1107/s0907444903016056DOI Listing
October 2003

Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.

Acta Crystallogr D Biol Crystallogr 2003 Sep 19;59(Pt 9):1670-3. Epub 2003 Aug 19.

Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 68790, Israel.

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http://dx.doi.org/10.1107/s0907444903014094DOI Listing
September 2003

Antibody recognition of chiral surfaces. Enantiomorphous crystals of leucine-leucine-tyrosine.

J Am Chem Soc 2003 Jan;125(3):696-704

Department of Structural Biology, Weizmann Institute of Science, 76100 Rehovot, Israel.

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http://pubs.acs.org/doi/abs/10.1021/ja027942j
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http://dx.doi.org/10.1021/ja027942jDOI Listing
January 2003

Alliin lyase (alliinase) from garlic (Allium sativum): crystallization and preliminary X-ray characterization.

Acta Crystallogr D Biol Crystallogr 2002 Aug 20;58(Pt 8):1335-7. Epub 2002 Jul 20.

Department of Chemical Services, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1107/s0907444902010466DOI Listing
August 2002

Thermophilic alcohol dehydrogenase from the mesophile Entamoeba histolytica: crystallization and preliminary X-ray characterization.

Acta Crystallogr D Biol Crystallogr 2002 Mar 21;58(Pt 3):546-8. Epub 2002 Feb 21.

Department of Chemical Services, The Weizmann Institute of Science, Rehovot 76100, Israel.

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http://dx.doi.org/10.1107/s0907444902001245DOI Listing
March 2002

Formation and X-ray Structures of PCP Ligand Based Platinum(II) and Palladium(II) Macrocycles.

Inorg Chem 1996 Nov;35(24):7068-7073

Departments of Organic Chemistry and Chemical Services, The Weizmann Institute of Science, 76100 Rehovot, Israel.

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http://dx.doi.org/10.1021/ic960285+DOI Listing
November 1996