Publications by authors named "Felipe de Oliveira Santos"

3 Publications

  • Page 1 of 1

Beneficial effects of losartan or telmisartan on the local hepatic renin-angiotensin system to counter obesity in an experimental model.

World J Hepatol 2019 Apr;11(4):359-369

Laboratório de Morfometria, Metabolismo e Doenças Cardiovasculares, Departamento de Anatomia, Instituto de Biologia Roberto Alcântara Gomes, Rio de Janeiro 20551-030, Brazil.

Background: Obesity has been associated with hepatic overexpression of the renin-angiotensin system (RAS).

Aim: To evaluate the action of two angiotensin II (ANGII) receptor blockers (losartan or telmisartan) on the modulation of local hepatic RAS and the resulting metabolic effects in a diet-induced obesity murine model.

Methods: Twenty C57BL/6 mice were randomly divided into two nutritional groups for 10 wk: control group (C, = 5, 10% of energy as fat) or high-fat group (HF, = 15, 50% of energy as fat). After treatment started, the HF group was randomly divided into three groups: untreated HF group ( = 5), HF treated with losartan (HFL, = 5) and HF treated with telmisartan (HFT, = 5). The treatments lasted for 5 wk, and the dose was 10 mg/kg body mass.

Results: HF diet induced body mass gain (+28%, < 0.0001), insulin resistance (+69%, = 0.0079), high hepatic triacylglycerol (+127%, = 0.0004), and overexpression of intrahepatic angiotensin-converting enzyme (ACE) 1/ ANGII type 1 receptor (AT1r) (+569.02% and +141.40%, respectively, < 0.0001). The HFL and HFT groups showed higher ACE2/rMAS gene expression compared to the HF group (ACE2: +465.57%, = 0.0002 for HFL and +345.17%, = 0.0049 for HFT; rMAS: +711.39%, < 0.0001 for HFL and +539.75%, < 0.0001 for HFT), followed by reduced insulin/glucose ratio (-30% for HFL and -33% for HFT, = 0.0181), hepatic triacylglycerol levels (-28%, = 0.0381 for HFL; and -45%, = 0.0010 for HFT, and Plin2 expression.

Conclusion: Modulation of the intrahepatic RAS, with favored involvement of the ACE2/rMAS axis over the ACE1/AT1r axis after losartan or telmisartan treatments, caused hepatic and metabolic beneficial effects as demonstrated by reduced hepatic triacylglycerol levels coupled with reduced PLIN 2 expression and improved glycemic control.
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http://dx.doi.org/10.4254/wjh.v11.i4.359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504859PMC
April 2019

AT1 receptor antagonist induces thermogenic beige adipocytes in the inguinal white adipose tissue of obese mice.

Endocrine 2017 Mar 23;55(3):786-798. Epub 2016 Dec 23.

Laboratory of Morphometry, Metabolism and Cardiovascular disease, Biomedical Centre, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

Purpose: To evaluate whether losartan is able to induce beige adipocytes formation, focusing on the thermogenic gene expression and adipocyte remodeling in the subcutaneous white adipose tissue of diet-induced obese mice.

Methods: Male C57BL/6 mice received a control diet (10% energy as lipids) or a high-fat diet (50% energy as lipids) for 10 weeks, followed by a 5-week treatment with losartan: control group, control-losartan group (10 mg/Kg/day), high-fat group and high-fat-losartan group (10 mg/Kg/day). Biochemical, morphometrical, stereological and molecular approaches were used to evaluate the outcomes.

Results: The high-fat diet elicited overweight, insulin resistance and adipocyte hypertrophy in the high-fat group, all of which losartan rescued in the high-fat-losartan group. These effects comply with the induction of beige adipocytes within the inguinal fat pads in high-fat-losartan group as they exhibited the greatest energy expenditure among the groups along with the presence uncoupling protein 1 positive multilocular adipocytes with enhanced peroxisome proliferator-activated receptor gamma coactivator 1-alpha and PR domain containing 16 mRNA levels, indicating a significant potential for mitochondrial biogenesis and adaptive thermogenesis.

Conclusions: Our results show compelling evidence that losartan countered diet-induced obesity in mice by enhancing energy expenditure through beige adipocytes induction. Reduced body mass, increased insulin sensitivity, decreased adipocyte size and marked expression of uncoupling protein 1 by ectopic multilocular adipocytes support these findings. The use of losartan as a coadjutant medicine to tackle obesity and its related disorders merits further investigation.
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http://dx.doi.org/10.1007/s12020-016-1213-1DOI Listing
March 2017

Quality of life in hypertensive patients and concurrent validity of Minichal-Brazil.

Arq Bras Cardiol 2010 Mar;94(3):337-44, 357-64

Universidade Federal do Paraná, Curitiba, PR, Brasil.

Background: Health-Related Quality of Life Questionnaires (HRQOL) are humanistic outcome measure instruments both in clinical and in pharmacoeconomic studies. However, they should have their psychometric parameters evaluated so as to reflect the individual subjective assessment of quality of life.

Objectives: To describe the quality of life profile of hypertensive patients and to assess the concurrent validity of the Minichal-Brazil instrument by comparing it to the generic World Health Organization's (WHO) assessment instrument known as WHOQOL-Bref.

Methods: A total of 191 adult patients (72.8% females) with hypertension were interviewed. Approximately one third of these patients had their hypertension controlled. The mean HRQOL as measured by the total Minichal score was 69.7 (SD = 19.2; 95%CI from 66.9 to 72.4); the "mental status" domain and "somatic manifestations" domain scores were 69.1 (95%CI from 66.1 to 72.2), and 69.9 (95%CI from 66.5 to 73.2), respectively. The means for the WHOQOL-Bref instrument were: in the "physical" domain = 61.5 (95%CI from 59.0 to 64.1); in the "psychological" domain = 65.7 (95%CI from 63.2 to 68.2); in the "social" domain = 72.3 (95%CI from 70.0 to 74.5); and in the "environment" domain = 59.7 (95%CI from 57.7 to 61.7).

Results: Minichal significantly correlated (p<0.001) with WHOQOL-Bref as regards all its domains, except for the "environment" domain, which did not correlate with the "somatic manifestations" domain.

Conclusion: Minichal-Brazil proved to be a useful tool in the assessment of HRQOL in hypertensive patients.
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http://dx.doi.org/10.1590/s0066-782x2010000300013DOI Listing
March 2010
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