Publications by authors named "Fei Zhang"

761 Publications

Expression Analysis of Circular RNAs in Young and Sexually Mature Boar Testes.

Animals (Basel) 2021 May 17;11(5). Epub 2021 May 17.

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Testicular development is critical for male animals' reproduction and is tightly regulated by epigenetic factors. Circular RNAs (circRNAs) were recently identified in the testes of humans and bulls. However, the expression profile of circRNAs and their potential biological functions in boar testicular development remain unclear. We identified 34,521 and 31,803 circRNAs in piglet (30 d) and adult (210 d) boar testes by high-throughput sequencing, respectively. Bioinformatics analysis revealed that these circRNAs are widely distributed on autosomes and sex chromosomes. Some of the host genes can generate multiple circRNAs. A total of 2326 differentially expressed circRNAs (DECs) derived from 1526 host genes was found in testicular development, of which 1003 circRNAs were up-regulated in adult boar testes and 1323 circRNAs were down-regulated. Furthermore, gene ontology analysis of host genes of DECs revealed that these circRNAs are mainly involved in regulating spermatogenesis, cilia motility, and hormone biosynthesis. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the DECs are markedly enriched to stem cell pluripotency regulation, tight junctions, adhesion junctions, and cAMP signaling pathway. These results indicate that circRNAs are abundantly expressed in boar testes and exhibit dynamic changes during testicular development.
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http://dx.doi.org/10.3390/ani11051430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156704PMC
May 2021

Compositions, sources, and potential health risks of volatile organic compounds in the heavily polluted rural North China Plain during the heating season.

Sci Total Environ 2021 May 23;789:147956. Epub 2021 May 23.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science & Engineering, Fudan University, Shanghai 200438, China; IRDR International Center of Excellence on Risk Interconnectivity and Governance on Weather/Climate Extremes Impact and Public Health, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China. Electronic address:

Severe volatile organic compound (VOC) pollution has become an urgent problem during the heating season in the North China Plain (NCP), as exposure to hazardous VOCs can lead to chronic or acute diseases. A campaign with online VOC measurements was conducted at a rural site in Wangdu, NCP during the 2018 heating season to characterize the compositions and associated sources of VOCs and to assess their potential health risks. The total concentration of VOCs with 94 identified species was 77.21 ± 54.39 ppb. Seven source factors were identified by non-negative matrix factorization, including coal combustion (36.1%), LPG usage (21.1%), solvent usage (13.9%), biomass burning and secondary formation (14.2%), background (7.0%), industrial emissions (4.5%), and vehicle emissions (3.3%). The point estimate approach and Monte Carlo simulation were used to estimate the carcinogenic and non-carcinogenic risks of harzadous VOCs. The results showed that the cumulative health risk of VOCs was above the safety level. Acrolein, 1.2-dichloroethane, 1,2-dichloropropane, chloroform, 1,3-butadiene, and benzene were identified as the key hazardous VOCs in Wangdu. Benzene had the highest average carcinogenic risk. Solvent usage and secondary formation were the dominant sources of adverse health effects. During the Spring Festival, most sources were sharply reduced; and VOC concentration declined by 49%. However, coal and biomass consumptions remained relatively large, probably due to heating demand. This study provides important references for the control strategies of VOCs during the heating season in heavily polluted rural areas in the NCP.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147956DOI Listing
May 2021

Two-dimensional ZrC as a novel anode material with high capacity for sodium ion battery.

Phys Chem Chem Phys 2021 Jun 26;23(22):12731-12738. Epub 2021 May 26.

College of Physics, Guizhou University, Guiyang 550025, People's Republic of China.

Rational design of high-performance anode materials is of paramount importance for developing rechargeable lithium ion batteries (LIBs) and sodium ion batteries (SIBs). In this work, a ZrC monolayer is predicted by performing the particle swarm optimization (PSO) algorithm. The high energetic, dynamic, and thermal stabilities of the ZrC monolayer are confirmed by cohesive energy, phonon dispersion, and molecular dynamics simulations, respectively. Unexpectedly, we find that the theoretical specific capacity for Na on the ZrC monolayer reaches as high as 932 mA h g, which is even higher than that of Li. Meanwhile, the diffusion energy barrier of Na on the ZrC monolayer is only 0.02 eV, ensuring the ultrafast charge/discharge rate. Additionally, the calculated open-circuit voltage (OCV) suggests that the change of Na intercalation voltage is steady. Therefore, our results consistently demonstrate that the ZrC monolayer can be an ideal anode material for SIBs.
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http://dx.doi.org/10.1039/d1cp00050kDOI Listing
June 2021

Particle-Phase Photoreactions of HULIS and TMIs Establish a Strong Source of HO and Particulate Sulfate in the Winter North China Plain.

Environ Sci Technol 2021 May 21. Epub 2021 May 21.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science and Engineering, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China.

During haze periods in the North China Plain, extremely high NO concentrations have been observed, commonly exceeding 1 ppbv, preventing the classical gas-phase HO formation through HO recombination. Surprisingly, HO mixing ratios of about 1 ppbv were observed repeatedly in winter 2017. Combined field observations and chamber experiments reveal a photochemical in-particle formation of HO, driven by transition metal ions (TMIs) and humic-like substances (HULIS). In chamber experiments, steady-state HO mixing ratios of 116 ± 83 pptv were observed upon the irradiation of TMI- and HULIS-containing particles. Correspondingly, HO formation rates of about 0.2 ppbv h during the initial irradiation periods are consistent with the HO rates observed in the field. A novel chemical mechanism was developed explaining the in-particle HO formation through a sequence of elementary photochemical reactions involving HULIS and TMIs. Dedicated box model studies of measurement periods with relative humidity >50% and PM ≥ 75 μg m agree with the observed HO concentrations and time courses. The modeling results suggest about 90% of the particulate sulfate to be produced from the SO reaction with OH and HSO oxidation by HO. Overall, under high pollution, the HO-caused sulfate formation rate is above 250 ng m h, contributing to the sulfate formation by more than 70%.
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http://dx.doi.org/10.1021/acs.est.1c00561DOI Listing
May 2021

Ratiometric fluorescence detection of sulfide ions based on lanthanide coordination polymer using guanosine diphosphate as ligand.

Colloids Surf B Biointerfaces 2021 Apr 28;204:111796. Epub 2021 Apr 28.

Jiangxi Key Laboratory of Industrial Ceramics, College of Materials and Chemical Engineering, Pingxiang University, Pingxiang, 337055, China.

The efficiency of energy transfer from guanine nucleotide to terbium ion (Tb) is affected by the phosphate group significantly. Compared with the biomolecules 5'-GMP (guanosine monophosphate), guanosine diphosphate (GDP) exhibits better sensitize ability to Tb ions luminescence. Assisted with the carboxycoumarin ligand, we synthesized a more stable optical [email protected] polymer with the characteristic emission peaks located on 440 nm and 545 nm in this work. The [email protected] polymer is not only rich in metal binding sites, but also maintains a moderate ionic binding force, which helps metal ions to bind or leave it easily. Experiment result shows that [email protected] polymer has the appropriate binding force for Fe ions, which can be destroyed by sulfur ions (S) as the formation of FeS precipitation. Based on this, [email protected] was designed as the ratio fluorescence probe for sulfur ions detection, where the fluorescence at 545 nm can be selectively quenched by Fe ions, while that at 440 nm was unaffected, in the presence of S ions, the quenched fluorescence can be recovered remarkably. With the increasing S ions from 0.1-45 μM, the ratio of fluorescence intensity at 545 nm to 440 nm (F/F) is linear to S concentration, and the detection limit of S was calculated to be 0.073 μM. Contrast to those fluorescence probes with single wavelength emission, [email protected] displays a comparable sensitivity, the introduced self-adjust wavelength improved the detection accuracy efficiently. The above 98.1 % recovery rates of S ions in the actual water sample demonstrated the practicability of [email protected] fluorescence probe.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111796DOI Listing
April 2021

Author Correction: Periostin Upregulates Wnt/β-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells.

Sci Rep 2021 Apr 29;11(1):9598. Epub 2021 Apr 29.

Department of Orthopaedics, National & Regional United Engineering Laboratory, Southwest Hospital, Third Military Medical University, Chongqing, China.

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http://dx.doi.org/10.1038/s41598-021-89264-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085006PMC
April 2021

Study on Corrosion Mechanism of Different Concentrations of NaSO Solution on Early-Age Cast-In-Situ Concrete.

Materials (Basel) 2021 Apr 16;14(8). Epub 2021 Apr 16.

Shanxi Qinhan Hengsheng New Building Materials Science and Technology Ltd., Xianyang 712000, China.

The deterioration of early-age concrete performance caused by SO internal diffusion in concrete is a critical factor of concrete durability. In this study, the mechanical properties, heat of hydration, and pore structure of early-age cast-in-situ concrete with different sodium sulfate (NaSO) concentrations were studied. The mechanism of SO internal corrosion was evaluated by measuring the dynamic elastic modulus, compressive strength, and heat of hydration rate. Scanning electron microscopy, energy dispersive spectroscopy, X-ray computed tomography, X-ray diffraction, thermogravimetry-derivative thermogravimetry, and differential scanning calorimetry were applied to analyze microstructural variations and complex mineral assemblages of concrete samples. The results indicated that during the hardening process of cast-in-situ concrete, NaSO first promoted and then hindered the hydration rate of cement, and also hindered the early strength development of the cement. As the concentration of NaSO solution increases, the corrosion products of ettringite (AFt) and gypsum (Gyp) gradually increase, causing cross cracks in the concrete. The proportion of small and medium pores first increases and then decreases, and the large pores first decrease and then increase. The mechanical properties of concrete gradually decrease and diminish the mechanical properties of the concrete (thereby accelerating the damage to the concrete).
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http://dx.doi.org/10.3390/ma14082018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073549PMC
April 2021

A simple strategy for constructing PET fluorescent probe and its application in hypochlorite detection.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 20;258:119827. Epub 2021 Apr 20.

Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

It is great meaningful to develop a fast and efficient method for detecting hypochlorite (ClO) owing to its importance in the immune system. In this work, we proposed a strategy to construct fluorescent probes for ClO based on photoinduced electron transfer (PET) mechanism. According to the strategy, we developed four fluorescent probes named TPA-NO, TPA-2NO, TPB-NO and TPB-2NO, and studied their detecting performances for hypochlorite. Among them, TPB-NO displayed the most obvious fluorescence changes towards ClO with a rapid response (<90 s). The detection limit was calculated to be 0.36 μM. Moreover, probe TPB-NO was successfully used to detect ClO in living cells and zebrafish. These results demonstrated the feasibility of our strategy and provided a guidance for developing more excellent probes in the future.
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http://dx.doi.org/10.1016/j.saa.2021.119827DOI Listing
September 2021

Drug-resistant cancer cell-derived exosomal EphA2 promotes breast cancer metastasis via the EphA2-Ephrin A1 reverse signaling.

Cell Death Dis 2021 Apr 20;12(5):414. Epub 2021 Apr 20.

Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, 300060, China.

Tumor metastasis induced by drug resistance is a major challenge in successful cancer treatment. Nevertheless, the mechanisms underlying the pro-invasive and metastatic ability of drug resistance remain elusive. Exosome-mediated intercellular communications between cancer cells and stromal cells in tumor microenvironment are required for cancer initiation and progression. Recent reports have shown that communications between cancer cells also promote tumor aggression. However, little attention has been regarded on this aspect. Herein, we demonstrated that drug-resistant cell-derived exosomes promoted the invasion of sensitive breast cancer cells. Quantitative proteomic analysis showed that EphA2 was rich in exosomes from drug-resistant cells. Exosomal EphA2 conferred the invasive/metastatic phenotype transfer from drug-resistant cells to sensitive cells. Moreover, exosomal EphA2 activated ERK1/2 signaling through the ligand Ephrin A1-dependent reverse pathway rather than the forward pathway, thereby promoting breast cancer progression. Our findings indicate the key functional role of exosomal EphA2 in the transmission of aggressive phenotype between cancer cells that do not rely on direct cell-cell contact. Our study also suggests that the increase of EphA2 in drug-resistant cell-derived exosomes may be an important mechanism of chemotherapy/drug resistance-induced breast cancer progression.
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http://dx.doi.org/10.1038/s41419-021-03692-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058342PMC
April 2021

Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer.

Mediators Inflamm 2021 1;2021:8856326. Epub 2021 Apr 1.

School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Non-small-cell lung cancer (NSCLC) remains the most common malignancy with the highest morbidity and mortality worldwide. In our previous study, we found that a classic traditional Chinese medicine (TCM) formula Ze-Qi-Tang (ZQT), which has been used in the treatment of respiratory diseases for thousands of years, could directly inhibit the growth of human NSCLC cells via the p53 signaling pathway. In this study, we explored the immunomodulatory functions of ZQT. We found that ZQT significantly prolonged the survival of orthotopic lung cancer model mice by modulating the tumor microenvironment (TME). ZQT remarkably reduced the number of MDSCs (especially G-MDSCs) and inhibited their immunosuppressive activity by inducing apoptosis in these cells via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. When G-MDSCs were depleted, the survival promotion effect of ZQT and its inhibitory effect on lung luminescence signal disappeared in tumor-bearing mice. This is the first study to illustrate the immunomodulatory effect of ZQT in NSCLC and the underlying molecular mechanism.
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http://dx.doi.org/10.1155/2021/8856326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035014PMC
April 2021

Identification of Metabolic-Associated Genes for the Prediction of Colon and Rectal Adenocarcinoma.

Onco Targets Ther 2021 31;14:2259-2277. Epub 2021 Mar 31.

Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin, 300060, People's Republic of China.

Background And Aim: Uncontrolled proliferation is the most prominent biological feature of tumors. In order to rapidly proliferate, tumor cells regulate their metabolic behavior by controlling the expression of metabolism-related genes (MRGs) to maximize the utilization of available nutrients. In this study, we aimed to construct prognosis models for colorectal adenocarcinoma (COAD) and rectum adenocarcinoma (READ) using MRGs to predict the prognoses of patients.

Methods: We first acquired the gene expression profiles of COAD and READ from the TCGA database, and then utilized univariate Cox analysis, Lasso regression, and multivariable Cox analysis to identify the MRGs for risk models.

Results: Eight genes (, and ) in the colon cancer risk model and six genes (, and ) in the rectal cancer risk model were identified successfully. Multivariate Cox analysis indicated that these two models could accurately and independently predict overall survival (OS) for patients with COAD or READ. Furthermore, functional enrichment analysis was used to identify the metabolism pathway of MRGs in the risk models and analyzed these genes comprehensively. Then, we verified the prognosis model in independent COAD cohorts (GSE17538) and detected the correlations of the protein expression levels of GSR and ENPP2 with prognosis for COAD or READ.

Conclusion: In this study, 14 MRGs were identified as potential prognostic biomarkers and therapeutic targets for colorectal cancer.
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http://dx.doi.org/10.2147/OTT.S297134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020594PMC
March 2021

Maternal and neonatal outcomes after energy-restricted diet for women with gestational diabetes mellitus: A systematic review and meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2021 Apr;100(14):e25279

Department of Obstetrics, Zaozhuang Maternity and Child Health Care Hospital, Zaozhuang, Shandong, P.R. China.

Background: Gestational diabetes mellitus (GDM) affects 1% to 14% of pregnant women annually worldwide and is one of the most common pregnancy complications.

Objective: We reviewed studies on maternal and neonatal outcomes after dietary managements for women with GDM comparing caloric-restricted (intervention group) and unrestricted diets (control group).

Methods: We systematically searched online databases including Medline, Cochrane Controlled Register of Trials (CENTRAL), ScienceDirect, and Google Scholar from inception until September 2019. We performed a meta-analysis with random-effects model and reported pooled risk ratios (RRs) or pooled mean differences (MD) with 95% confidence intervals (CIs).

Results: We analyzed data from 6 randomized controlled trials including 1300 participants, most of them with high bias risks. We found that the women in the intervention group achieved slightly better glycemic control (pooled MD, -0.72 mg/dL; 95% CI, -7.10 to 5.66 mg/dL) and overall pregnancy outcomes (except neonatal hypoglycemia) than the women in the control group.

Conclusion: An energy-restricted diet does not seem superior to the usual/standard GDM diet based on maternal or neonatal outcomes. But, clinical recommendations cannot be made as the evidence is inconclusive.
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http://dx.doi.org/10.1097/MD.0000000000025279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036084PMC
April 2021

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Aging (Albany NY) 2021 04 4;13(8):11281-11295. Epub 2021 Apr 4.

Department of Rehabilitation Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Huangpu 200011, Shanghai, People's Republic of China.

Background: Developmental dysplasia of the hip (DDH) is the most common joint disease in child orthopedics. Secreted Frizzled-Related Protein 3 (FRZB) plays an important role in joint development. however, no direct association between and DDH has been demonstrated.

Methods: Analysis of genotype distribution and allele frequency for detected single nucleotide polymorphisms (SNP) of was performed. expression was assayed in DDH joint tissues. Further experiments to identify the chondrogenic properties of were conducted. Potential upstream miRNAs for were assayed in DDH.

Results: Significant difference in genotype distribution for rs3768842 (OR=1.46, P=0.0081) and rs2242040 (OR=0.65, P=0.0067) was found. DDH joint tissues showed significantly higher expression. demonstrated chondrogenic and anti-hypertrophic properties . modulated cell adhesion pathway and cell spreading by regulating integrins expressions. Upstream miRNAs regulating FRZB expression were identified in DDH synovial fluid. Experiments indicated that downregulated miRNA-454 caused FRZB upregulation in DDH joint.

Conclusion: Dysregulated and its loci were associated with DDH. As a Wnt antagonist with chondrogenic properties, modulated cell adhesion pathway and cell spreading by regulating integrins expressions. FRZB in multiple DDH joint tissues might be mediated by the dysregulated miRNA expression profiles in the joint synovial fluid.
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http://dx.doi.org/10.18632/aging.202815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109121PMC
April 2021

A TICT + AIE based fluorescent probe for ultrafast response of hypochlorite in living cells and mouse.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 23;256:119735. Epub 2021 Mar 23.

Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

Hypochlorite (HClO/ClO), an important reactive oxygen species (ROS), plays a significant role in the human immune system. Thus, developing a fast and efficient method for detecting ClO is quite necessary. Herein, we designed and synthesized a fluorescent probe TPB-CN based on twisted intramolecular charge transfer (TICT) and aggregation-induced emission (AIE) characteristics. The probe could respond to ClO with an ultrafast response velocity (<2 s). The detection limit was calculated to be 6.198 nM. In addition, probe TPB-CN was successfully applied for detecting ClO in living cells and mouse.
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http://dx.doi.org/10.1016/j.saa.2021.119735DOI Listing
July 2021

Laser surface texturing of zirconia-based ceramics for dental applications: A review.

Mater Sci Eng C Mater Biol Appl 2021 Apr 11;123:112034. Epub 2021 Mar 11.

KU Leuven, Department of Mechanical Engineering and Flanders [email protected] Leuven-MaPS, Celestijnenlaan 300, 3001 Leuven, Belgium. Electronic address:

Laser surface texturing is widely explored for modifying the surface topography of various materials and thereby tuning their optical, tribological, biological, and other surface properties. In dentistry, improved osseointegration has been observed with laser textured titanium dental implants in clinical trials. Due to several limitations of titanium materials, dental implants made of zirconia-based ceramics are now considered as one of the best alternatives. Laser surface texturing of zirconia dental implants is therefore attracting increasing attention. However, due to the brittle nature of zirconia, as well as the metastable tetragonal ZrO phase, laser texturing in the case of zirconia is more challenging than in the case of titanium. Understanding these challenges requires different fields of expertise, including laser engineering, materials science, and dentistry. Even though much progress was made within each field of expertise, a comprehensive analysis of all the related factors is still missing. This review paper provides thus an overview of the common challenges and current status on the use of lasers for surface texturing of zirconia-based ceramics for dental applications, including texturing of zirconia implants for improving osseointegration, texturing of zirconia abutments for reducing peri-implant inflammation, and texturing of zirconia restorations for improving restoration retention by bonding.
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http://dx.doi.org/10.1016/j.msec.2021.112034DOI Listing
April 2021

Creatine promotes cancer metastasis through activation of Smad2/3.

Cell Metab 2021 Jun 2;33(6):1111-1123.e4. Epub 2021 Apr 2.

Key Laboratory of RNA Biology, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Center for Excellence in Biomacromolecules, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

As one of the most popular nutrient supplements, creatine has been highly used to increase muscle mass and improve exercise performance. Here, we report an adverse effect of creatine using orthotopic mouse models, showing that creatine promotes colorectal and breast cancer metastasis and shortens mouse survival. We show that glycine amidinotransferase (GATM), the rate-limiting enzyme for creatine synthesis, is upregulated in liver metastases. Dietary uptake, or GATM-mediated de novo synthesis of creatine, enhances cancer metastasis and shortens mouse survival by upregulation of Snail and Slug expression via monopolar spindle 1 (MPS1)-activated Smad2 and Smad3 phosphorylation. GATM knockdown or MPS1 inhibition suppresses cancer metastasis and benefits mouse survival by downregulating Snail and Slug. Our findings call for using caution when considering dietary creatine to improve muscle mass or treat diseases and suggest that targeting GATM or MPS1 prevents cancer metastasis, especially metastasis of transforming growth factor beta receptor mutant colorectal cancers.
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http://dx.doi.org/10.1016/j.cmet.2021.03.009DOI Listing
June 2021

A Robot Object Recognition Method Based on Scene Text Reading in Home Environments.

Sensors (Basel) 2021 Mar 9;21(5). Epub 2021 Mar 9.

School of Information Science and Technology, Northeast Normal University, Changchun 130117, China.

With the aim to solve issues of robot object recognition in complex scenes, this paper proposes an object recognition method based on scene text reading. The proposed method simulates human-like behavior and accurately identifies objects with texts through careful reading. First, deep learning models with high accuracy are adopted to detect and recognize text in multi-view. Second, datasets including 102,000 Chinese and English scene text images and their inverse are generated. The F-measure of text detection is improved by 0.4% and the recognition accuracy is improved by 1.26% because the model is trained by these two datasets. Finally, a robot object recognition method is proposed based on the scene text reading. The robot detects and recognizes texts in the image and then stores the recognition results in a text file. When the user gives the robot a fetching instruction, the robot searches for corresponding keywords from the text files and achieves the confidence of multiple objects in the scene image. Then, the object with the maximum confidence is selected as the target. The results show that the robot can accurately distinguish objects with arbitrary shape and category, and it can effectively solve the problem of object recognition in home environments.
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http://dx.doi.org/10.3390/s21051919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967195PMC
March 2021

The complete chloroplast genome sequence of (Gaertn.) DC. ().

Mitochondrial DNA B Resour 2021 Mar 11;6(3):769-770. Epub 2021 Mar 11.

Pharmacy College of Henan University of Chinese Medicine, Zhengzhou, China.

In this study, we constructed and annotated a complete circular chloroplast genome of wild . The chloroplast genome of wild is 153,678 bp in length, including two inverted repeat (IR) regions of 25,759 bp, separated by a large single copy (LSC) region of 84,544 bp and a small single copy (SSC) region of 17,616 bp. The genome contains 149 genes, including 104 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Neighbor-joining method phylogenomic analysis showed that wild formed a monophyletic group, and was sister to other groups of .
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http://dx.doi.org/10.1080/23802359.2021.1881837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954418PMC
March 2021

CENTRORADIALIS maintains shoot meristem indeterminacy by antagonizing THORN IDENTITY1 in Citrus.

Curr Biol 2021 May 23;31(10):2237-2242.e4. Epub 2021 Mar 23.

Department of Molecular, Cellular and Developmental Biology, Yale University, 260 Whitney Avenue, New Haven, CT 06520, USA; Department of Ecology and Evolutionary Biology, Yale University, 165 Prospect Street, New Haven, CT 06520, USA. Electronic address:

Differential regulation of stem cell activity in shoot meristems contributes to the wide variation in shoot architecture. In most Citrus species, a thorn meristem and a dormant axillary meristem co-localize at each leaf base, offset from each other in a spiral phyllotactic pattern. We recently identified THORN IDENTITY1 (TI1) and THORN IDENTITY2 (TI2), encoding TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors, as necessary for the termination of meristem proliferation and concomitant thorn production in Citrus. However, how the dormant axillary meristem at the same leaf axil maintains stem cell activity is still unknown. The phosphatidylethanolamine-binding protein (PEBP)-type transcription factors CENTRORADIALIS (CEN) and TERMINAL FLOWER1 (TFL1) maintain inflorescence meristem indeterminacy in many plant species by antagonizing floral meristem identity regulators. Here, we show that, in Citrus, Citrus CEN (CsCEN) maintains vegetative axillary meristem indeterminacy by antagonizing TI1. CsCEN is expressed in the axillary meristem, but not in the thorn meristem. Disruption of CsCEN function results in termination of the stem cell activity and conversion of dormant axillary meristems into thorns, although ectopic overexpression of CsCEN represses TI1 expression and converts thorns into dormant buds, a phenotype similar to the ti1 mutant. We further show that CsCEN interacts with Citrus FD (CsFD) to repress TI1 expression. CsCEN activity depends on the function of TI1 and TI2, as mutations in TI1 and TI2 rescue the cscen mutant phenotype. We suggest that the antagonistic roles of CsCEN and TI1 define the pattern of axillary meristem determinacy, which shapes vegetative Citrus tree shoot architecture.
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http://dx.doi.org/10.1016/j.cub.2021.02.051DOI Listing
May 2021

Research Progress on the Mechanism of Lumbarmultifidus Injury and Degeneration.

Oxid Med Cell Longev 2021 26;2021:6629037. Epub 2021 Feb 26.

Department of Spinal Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei Province, China.

This review summarizes recent research progress in the clinical features, image manifestations, and pathological mechanism of multifidus injury. After a brief introduction to the fiber classification, innervation, blood supply, and multifidus function, some factors of multifidus injury, consisting of denervation, intraoperative incision selection and traction, and lumbar degenerative disease are overviewed. In addition, the clinical index of multifidus injury including myoglobin, creatine kinase, IL-6, C-reactive protein, the cross-sectional area of multifidus, the degree of fat infiltration, and intraoperative biopsy are summarized. Furthermore, we recommend that patients with chronic low back pain should take the long-term exercise of lumbodorsal muscles. Finally, some remaining issues, including external fixation and the imaging quantitative evaluation criteria of multifidus, need to be further explored in the future.
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http://dx.doi.org/10.1155/2021/6629037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936897PMC
May 2021

How to Choose a Proper Theoretical Analysis Model Based on Cell Adhesion and Nonadhesion Impedance Measurement.

ACS Sens 2021 03 16;6(3):673-687. Epub 2021 Mar 16.

School of Electrical and Information Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

The accurate equivalent circuit model contributes to the better fitting of required cell characteristics, such as cell impedance, cell adhesion area, and cell-electrode distance. However, so many theoretical models on specific modules make it difficult for new researchers to understand the whole model of electrode system physically. Besides, the accurate theoretical model and the simplified calculations obviously contradict each other; therefore, it is confusing for many researchers to choose the proper theoretical model to calculate the specific parameters required. In this review, we first discuss the problems and suggestions of electrode system design for cell adhesion-based measurement in terms of parasitic capacitance, detection range of cell number, electric field distribution, and interelectrode distance. The design of electrode system for cell nonadhesion measurement was analyzed in terms of microchannel size and electrode position. Then, we discuss the advantages and disadvantages of various equivalent circuit models according to different requirements of researchers, and simultaneously provide a corresponding theoretical model for researchers. Various factors influencing electric impedance spectroscopy (EIS) such as the parasitic capacitance between microelectrodes, the changes of cell adhesion area and cell-electrode distance, the electrode geometry, and the surface conductivity of electrode were quantitatively analyzed to contribute to better understanding of the equivalent models. Finally, we gave advice to optimize the theoretical models further and perspectives on building uniform principles of theoretical model optimization in the future.
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http://dx.doi.org/10.1021/acssensors.0c02710DOI Listing
March 2021

Evaluating cell viability heterogeneity based on information fusion of multiple adhesion strengths.

Biotechnol Bioeng 2021 Jun 25;118(6):2360-2367. Epub 2021 Mar 25.

School of Electrical and Information Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.

Cell viability evaluation is significantly meaningful for cellular assays. Some cells with weak viability are easily killed in the detection of anticancer drugs, while others with strong viability survive and proliferate, ultimately leading to the treatment failure or the inaccuracy of biological assays. Accurately evaluating cell viability heterogeneity still remains difficult. This article proposed a multiphysical property information fusion method for evaluating cell viability heterogeneity based on polynomial regression in a single-channel integrated microfluidic chip. In this method, adhesion strengths τ , that are defined as the magnitude of shear stress needed to detach (100 - N) % of cell population, were extracted as the independent variables of polynomial regression model by calculating the nonlinear fitting of the impedance-response curves for shear stress (cell detachment assay). Besides, by calculating the nonlinear fitting of the drug dose-response curves for cancer cells (IC assay), the half-maximal inhibitory concentration (IC ) was extracted as the dependent variables of polynomial regression model. The results show that the mean relative error of our fusion method averagely reduces by 6.04% and 62.79% compared with the multiple linear regression method and the cell counting method. Moreover, a simplified theoretical model used to describe the quantitative relationship between cell viability and its adhesion strengths was built to provide a theoretical basis for our fusion method.
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http://dx.doi.org/10.1002/bit.27749DOI Listing
June 2021

Quasi-Continuous Metasurface Beam Splitters Enabled by Vector Iterative Fourier Transform Algorithm.

Materials (Basel) 2021 Feb 21;14(4). Epub 2021 Feb 21.

State Key Laboratory of Optical Technologies on Nano-Fabrication and Micro-Engineering, Institute of Optics and Electronics, Chinese Academy of Sciences, P.O. Box 350, Chengdu 610209, China.

Quasi-continuous metasurfaces are widely used in various optical systems and their subwavelength structures invalidate traditional design methods based on scalar diffraction theory. Here, a novel vector iterative Fourier transform algorithm (IFTA) is proposed to realize the fast design of quasi-continuous metasurface beam splitters with subwavelength structures. Compared with traditional optimization algorithms that either require extensive numerical simulations or lack accuracy, this method has the advantages of accuracy and low computational cost. As proof-of-concept demonstrations, several beam splitters with custom-tailored diffraction patterns and a 7 × 7 beam splitter are numerically demonstrated, among which the maximal diffraction angle reaches 70° and the best uniformity error reaches 0.0195, showing good consistency with the target energy distribution and these results suggest that the proposed vector IFTA may find wide applications in three-dimensional imaging, lidar techniques, machine vision, and so forth.
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http://dx.doi.org/10.3390/ma14041022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926747PMC
February 2021

The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer.

Front Oncol 2020 16;10:570916. Epub 2021 Feb 16.

Department of Surgical Oncology and General Surgery, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, The First Affiliated Hospital of China Medical University, Shenyang, China.

tRNA-derived fragments (tRFs) are a new classification of small non-coding RNAs (sncRNAs) derived from the specific cleavage of precursors and mature tRNAs. Accumulating recent evidence has shown that tRFs are frequently abnormal in several cancers. Nevertheless, the role of tRFs in gastric cancer and its mechanism remain unclear. In this study, we found abnormal expression of tRF-3017A (derived from tRNA-Val-TAC) in gastric cancer tissues and cell lines and confirmed its effect on promoting the invasion and migration of gastric cancer cells through functional experiments . Analysis of clinicopathologic data showed patients with higher tRF-3017A were associated with significantly higher lymph node metastasis. Mechanistic investigation implies that tRF-3017A regulates the tumor suppressor gene NELL2 through forming the RNA-induced silencing complex (RISC) with Argonaute (AGO) proteins. In this study, we found that higher tRF-3017A were associated with significantly higher lymph node metastasis in gastric cancer patients and the tRF-3017A may play a role in promoting the migration and invasion of gastric cancer cells by silencing tumor suppressor NELL2.
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http://dx.doi.org/10.3389/fonc.2020.570916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921707PMC
February 2021

The protein phosphatase PPM1A dephosphorylates and activates YAP to govern mammalian intestinal and liver regeneration.

PLoS Biol 2021 Feb 25;19(2):e3001122. Epub 2021 Feb 25.

MOE Laboratory of Biosystems Homeostasis & Protection, Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.

The Hippo-YAP pathway responds to diverse environmental cues to manage tissue homeostasis, organ regeneration, tumorigenesis, and immunity. However, how phosphatase(s) directly target Yes-associated protein (YAP) and determine its physiological activity are still inconclusive. Here, we utilized an unbiased phosphatome screening and identified protein phosphatase magnesium-dependent 1A (PPM1A/PP2Cα) as the bona fide and physiological YAP phosphatase. We found that PPM1A was associated with YAP/TAZ in both the cytoplasm and the nucleus to directly eliminate phospho-S127 on YAP, which conferring YAP the nuclear distribution and transcription potency. Accordingly, genetic ablation or depletion of PPM1A in cells, organoids, and mice elicited an enhanced YAP/TAZ cytoplasmic retention and resulted in the diminished cell proliferation, severe gut regeneration defects in colitis, and impeded liver regeneration upon injury. These regeneration defects in murine model were largely rescued via a genetic large tumor suppressor kinase 1 (LATS1) deficiency or the pharmacological inhibition of Hippo-YAP signaling. Therefore, we identify a physiological phosphatase of YAP/TAZ, describe its critical effects in YAP/TAZ cellular distribution, and demonstrate its physiological roles in mammalian organ regeneration.
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http://dx.doi.org/10.1371/journal.pbio.3001122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978383PMC
February 2021

Tunable NIR AIE-active optical materials for lipid droplet imaging in typical model organisms and photodynamic therapy.

J Mater Chem B 2021 03;9(10):2417-2427

Institute of Molecular Science, Engineering Research Center for Sewage Treatment of Shanxi Province, Shanxi University, Taiyuan, 030006, China.

Near infrared (NIR) luminescent materials with aggregation-induced emission (AIE) features have attracted enormous attention in the areas of medical imaging and diagnostic therapeutics because of their low background fluorescence and strong tissue penetration. This study reports a series of easily synthesized AIEgen molecules that feature NIR emission. These molecules have a donor-donor-π-acceptor (D1-D2-π-A) structure with intramolecular charge transfer (ICT) character. The nature of charge transfer transition can be modified by different structures of D2, i.e. phenyl, thiophene, and furan ring. These AIEgens have high selectivity towards lipid droplets (LDs) in vitro and in vivo, such as zebrafish, Caenorhabditis elegans, and oil crop tissue. In addition, the effect of photodynamic therapy (PDT) on SMMC-7721 cells was investigated, and the results indicate that these AIEgens have potential application for PDT on cancer cells with white light illumination. This study reveals that these triphenylamine (TPA)-based AIEgens have great potential for biological imaging and preclinical applications of PDT.
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http://dx.doi.org/10.1039/d0tb02801kDOI Listing
March 2021

Regional suitability prediction of soil salinization based on remote-sensing derivatives and optimal spectral index.

Sci Total Environ 2021 Jun 12;775:145807. Epub 2021 Feb 12.

Key Laboratory of Wisdom City and Environment Modeling of Higher Education Institute, College of Resources and Environmental Science, Xinjiang University, Urumqi 830046, China; Key Laboratory of Oasis Ecology, Xinjiang University, Urumqi 830046, China.

Soil salinization is an extremely serious land degradation problem in arid and semi-arid regions that hinders the sustainable development of agriculture and food security. Information and research on soil salinity using remote sensing (RS) technology provide a quick and accurate assessment and solutions to address this problem. This study aims to compare the capabilities of Landsat-8 OLI and Sentinel-2A MSI in RS prediction and exploration of the potential application of derivatives to RS prediction of salinized soils. It explores the ability of derivatives to be used in the Landsat-8 OLI and Sentinel-2A MSI multispectral data, and it was used as a data source as well as to address the adaptability of salinity prediction on a regional scale. The two-dimensional (2D) and three-dimensional (3D) optimal spectral indices are used to screen the bands that are most sensitive to soil salinity (0-10 cm), and RS data and topographic factors are combined with machine learning to construct a comprehensive soil salinity estimation model based on gray correlation analysis. The results are as follows: (1) The optimal spectral index (2D, 3D) can effectively consider possible combinations of the bands between the interaction effects and responding to sensitive bands of soil properties to circumvent the problem of applicability of spectral indices in different regions; (2) Both the Landsat-8 OLI and Sentinel-2A MSI multispectral RS data sources, after the first-order derivative techniques are all processed, show improvements in the prediction accuracy of the model; (3) The best performance/accuracy of the predictive model is for sentinel data under first-order derivatives. This study compared the capabilities of Landsat-8 OLI and Sentinel-2A MSI in RS prediction in finding the potential application of derivatives to RS prediction of salinized soils, with the results providing some theoretical basis and technical guidance for salinized soil prediction and environmental management planning.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145807DOI Listing
June 2021

Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis.

Genomics Proteomics Bioinformatics 2021 Feb 17. Epub 2021 Feb 17.

Department of Surgical Oncology and General Surgery, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, The First Affiliated Hospital of China Medical University, Shenyang 110001, China. Electronic address:

Gastric cancer (GC) is known as a top malignant type of tumors worldwide. Despite the recent decrease in mortality rates, the prognosis remains poor. Therefore, it is necessary to find novel biomarkers with early diagnostic value for GC. In this study, we present a large-scale proteomic analysis of 30 GC tissues and 30 matched healthy tissues using label-free global proteome profiling. Our results identified 537 differentially expressed proteins, including 280 upregulated and 257 downregulated proteins. The ingenuity pathway analysis (IPA) results indicated that the sirtuin signaling pathway was the most activated pathway in GC tissues whereas oxidative phosphorylation was the most inhibited. Moreover, the most activated molecular function was cellular movement, including tissue invasion by tumor cell lines. Based on IPA results, 15 hub proteins were screened. Using the receiver operating characteristic curve, most of hub proteins showed a high diagnostic power in distinguishing between tumors and healthy controls. A four-protein (ATP5B-ATP5O-NDUFB4-NDUFB8) diagnostic signature was built using a random forest model. The area under the curve (AUC) values of this model were 0.996 and 0.886 for the training and testing sets, respectively, suggesting that the four-protein signature has a high diagnostic power. This signature was further tested with independent datasets using plasma enzyme-linked immune sorbent assays, resulting in an AUC value of 0.778 for distinguishing GC tissues from healthy controls, and using immunohistochemical tissue microarray analysis, resulting in an AUC value of 0.805. In conclusion, this study identifies potential biomarkers and improves our understanding of the pathogenesis, providing novel therapeutic targets for GC.
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http://dx.doi.org/10.1016/j.gpb.2020.06.012DOI Listing
February 2021

Extreme-Angle Silicon Infrared Optics Enabled by Streamlined Surfaces.

Adv Mater 2021 Mar 10;33(11):e2008157. Epub 2021 Feb 10.

State Key Laboratory of Optical Technologies on Nano-Fabrication and Micro-Engineering, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, 610209, China.

Infrared optical systems are indispensable in almost all domains of society, but their performances are often restricted by bulky size, small field of view, large thermal sensitivity, high fabrication cost, etc. Here, based on the concept of catenary optics, a novel isophase streamline optimization approach is leveraged to design silicon complementary metal-oxide-semiconductor (CMOS)-compatible metasurfaces with broadband, wide-angle, and high-efficiency performances, which breaks through the glass ceiling of traditional optical technologies. By using the truly local geometric phase, a maximum diffraction efficiency approaching 100% is obtained in ultrawide spectral and angular ranges. Somewhat surprising results are shown in that wide-angle diffraction-limited imaging and laser beam steering can be realized with a record field of view up to 178°. This methodology is scalable to the entire optical band and other materials, enabling unprecedented compact infrared systems for surveillance, unmanned vehicles, medical science, etc.
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http://dx.doi.org/10.1002/adma.202008157DOI Listing
March 2021