Publications by authors named "Fei Yi"

146 Publications

Percutaneous Vertebroplasty for C1 Osteolytic Lesions via Lateral Approach Under Fluoroscopic Guidance.

J Pain Res 2021 13;14:2121-2128. Epub 2021 Jul 13.

Department of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

Objective: To report our experience of treating painful C1 osteolytic lesions with cement augmentation via a lateral approach under fluoroscopic guidance.

Materials And Methods: Nine consecutive patients (eight men and one woman; mean age: 56.7±13.2 years) with osteolytic lesions of the atlas who underwent cementoplasty via a lateral approach were enrolled in this study. The technical success rate, operation time, and complications were recorded. Visual analogue scale (VAS) and Neck disability index (NDI) were used to evaluate the pain relief and neck function status pre-procedure (baseline) and post-procedure (at 3 days; after 1, 3, 6, 12, 18, and 24 months; and at the last follow-up).

Results: The procedure was technically successful without any complications in all patients. The mean procedure time was 44.3±7.8 min. There were three cases of bone cement leakage, but no clinical symptoms. The mean VAS score decreased from 6.7±1.0 before the procedure to 3.7 three days after the procedure. The mean NDI score decreased from 67.3±11.2 before the procedure to 39.3±13.7 three days after the procedure. The VAS and NDI scores decreased at each follow-up time point, and the difference was statistically significant compared with the scores before the procedure (P<0.01).

Conclusion: Fluoroscopy-guided lateral approach is a feasible, safe, and effective method for treatment of osteolytic lesions of the atlas and can relieve pain and stabilize the bone.
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http://dx.doi.org/10.2147/JPR.S318236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286128PMC
July 2021

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis.

Int J Biol Sci 2021 23;17(7):1795-1807. Epub 2021 Apr 23.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China.

Emerging evidence has suggested the functions of exosomes in allergic diseases including asthma. By using a mouse model with asthma induced by ovalbumin (OVA), we explored the roles of M2 macrophage-derived exosomes (M2Φ-Exos) in asthma progression. M2Φ-Exos significantly alleviated OVA-induced fibrosis and inflammatory responses in mouse lung tissues, as well as inhibited abnormal proliferation, invasion, and fibrosis-related protein production in platelet derived growth factor (PDGF-BB) treated primary mouse airway smooth muscle cells (ASMCs). The OVA administration in mice or the PDGF-BB treatment in ASMCs reduced the expression of miR-370, which was detected in M2Φ-Exos by miRNA sequencing. However, treating the mice or ASMCs with M2Φ-Exos reversed the inhibitory effect of OVA or PDGF-BB on miR-370 expression. We identified that the target of miR-370 was fibroblast growth factor 1 (). Downregulation of miR-370 by Lv-miR-370 inhibitor or overexpression of FGF1 by Lv-FGF1 blocked the protective roles of M2Φ-Exos in asthma-like mouse and cell models. M2Φ-Exos were found to inactivate the MAPK signaling pathway, which was recovered by miR-370 inhibition or FGF1 overexpression. Collectively, we conclude that M2Φ-Exos carry miR-370 to alleviate asthma progression through downregulating FGF1 expression and the MAPK/STAT1 signaling pathway. Our study may offer a novel insight into asthma treatment.
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http://dx.doi.org/10.7150/ijbs.59715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120458PMC
April 2021

Bile Acids Impair Vaccine Response in Children With Biliary Atresia.

Front Immunol 2021 16;12:642546. Epub 2021 Apr 16.

Department of Liver Surgery and Liver Transplantation, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Vaccination is the best way to protect children under 5 years from death or disability. Children with biliary atresia (BA), which is the most common pediatric cholestatic end-stage liver disease (PELD), are more vulnerable to infectious diseases. However, the vaccination coverage and factors modulating vaccine responses in children with BA are largely unknown.

Methods: In this study, 288 children (median age: 7 months) diagnosed with BA before liver transplantation were enrolled for the evaluation of vaccination status and the factors affecting the immune response to the hepatitis B (HBV) vaccine. Moreover, 49 BA children (median age: 4 months) were enrolled for flow cytometric analysis of CD4 T cells and CD19 B cell subsets and correlations with serum bile acid levels.

Results: Generally, these children had very low routine vaccination rates for the meningococcal serogroup AC (Men AC) (41.2%), measles-mumps-rubella (MMR) (31.3%), poliomyelitis (Polio) (25.3%), hepatitis A (HAV) (25.0%), Japanese encephalitis (JE) (15.0%), diphtheria-tetanus-pertussis (DTP) (14.2%), meningococcal serogroup A (Men A) (13.5%) and varicella (VAR) (10.8%) vaccines, but not for the HBV (96.2%) and bacillus Calmette-Guérin (BCG) (84.7%) vaccines. Remarkably, 19.8% (57/288) of the patients had HBV infection. Out of 220 patients vaccinated for HBV, 113 (51.4%), 85 (38.6%) and 22 (10%) had one, two or three doses of the HBV vaccine, respectively. Furthermore, logistic regression analysis revealed that the bile acid level was an independent factor associated with poor HBV vaccine response ( = 0.03; OR = 0.394; 95% CI = 0.170-0.969). Immunophenotyping showed that bile acids were only negatively correlated with the CD19CD27IgG post-class-switched memory B cell ratio ( = 0.01).

Conclusion: This study reveals the overall vaccination rates of routine vaccines in Chinese BA children are very low and the poor HBV vaccine responses are associated with bile acids, possibly the inhibition of CD19CD27IgG post-class-switched memory B cell response.

Clinical Trial Registration: http://www.chictr.org.cn, identifier ChiCTR1800019165.
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http://dx.doi.org/10.3389/fimmu.2021.642546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085329PMC
April 2021

Transcriptome dynamic landscape underlying the improvement of maize lodging resistance under coronatine treatment.

BMC Plant Biol 2021 Apr 27;21(1):202. Epub 2021 Apr 27.

State Key Laboratory of Plant Physiology and Biochemistry, Engineering Research Center of Plant Growth Regulator, Ministry of Education &College of Agronomy and Biotechnology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, China.

Background: Lodging is one of the important factors causing maize yield. Plant height is an important factor in determining plant architecture in maize (Zea mays L.), which is closely related to lodging resistance under high planting density. Coronatine (COR), which is a phytotoxin and produced by the pathogen Pseudomonas syringae, is a functional and structural analogue of jasmonic acid (JA).

Results: In this study, we found COR, as a new plant growth regulator, could effectively reduce plant height and ear height of both hybrids (ZD958 and XY335) and inbred (B73) maize by inhibiting internode growth during elongation, thus improve maize lodging resistance. To study gene expression changes in internode after COR treatment, we collected spatio-temporal transcriptome of inbred B73 internode under normal condition and COR treatment, including the three different regions of internode (fixed, meristem and elongation regions) at three different developmental stages. The gene expression levels of the three regions at normal condition were described and then compared with that upon COR treatment. In total, 8605 COR-responsive genes (COR-RGs) were found, consist of 802 genes specifically expressed in internode. For these COR-RGs, 614, 870, 2123 of which showed expression changes in only fixed, meristem and elongation region, respectively. Both the number and function were significantly changed for COR-RGs identified in different regions, indicating genes with different functions were regulated at the three regions. Besides, we found more than 80% genes of gibberellin and jasmonic acid were changed under COR treatment.

Conclusions: These data provide a gene expression profiling in different regions of internode development and molecular mechanism of COR affecting internode elongation. A putative schematic of the internode response to COR treatment is proposed which shows the basic process of COR affecting internode elongation. This research provides a useful resource for studying maize internode development and improves our understanding of the COR regulation mechanism based on plant height.
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http://dx.doi.org/10.1186/s12870-021-02962-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077928PMC
April 2021

Voltage tunable mid-wave infrared reflective varifocal metalens via an optomechanic cavity.

Opt Lett 2021 Apr;46(8):1930-1933

Metalenses enable the multifunctional control of light beams with an optically thin layer of nanoantennas. Efficient on-chip voltage tuning of the focal length is the crucial step toward the integration of metalenses into dynamically tunable optical systems. We propose and numerically investigate the on-chip electrical tuning of a reflective metalens via an optomechanic cavity. Light is focused by an array of silicon nanopillar antennas separated from a deformable metallic reflector by a small air gap. A transparent electrode is inserted into the optomechanic cavity to electrostatically deform the reflector and rearrange the reflection phase profile, resulting in a shift in the focal point. Two modes of voltage tuning via the relative curvature change of the reflector are analyzed. In mode 1, the size of the air gap is modified through the nearly parallel shift of the reflector, whereas in mode 2, the distribution of the air-gap size is tailored by the curvature change of the reflector. With the designed working wavelength of 3.8 µm and the initial focal length of 80.35 µm, the focal length is shifted by 20.3 µm in mode 1 and 7.25 µm in mode 2. Such a device can be used as a free space coupler between quantum cascade lasers and mid-infrared fibers with variable coupling efficiency.
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http://dx.doi.org/10.1364/OL.417224DOI Listing
April 2021

Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K Uptake in Cotton Roots.

Int J Mol Sci 2021 Mar 19;22(6). Epub 2021 Mar 19.

State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100193, China.

To properly understand cotton responses to potassium (K) deficiency and how its shoot feedback regulates K uptake and root growth, we analyzed the changes in root transcriptome induced by low K (0.03 mM K, lasting three days) in self-grafts of a K inefficient cotton variety (CCRI41/CCRI41, scion/rootstock) and its reciprocal grafts with a K efficient variety (SCRC22/CCRI41). Compared with CCRI41/CCRI41, the SCRC22 scion enhanced the K uptake and root growth of CCRI41 rootstock. A total of 1968 and 2539 differently expressed genes (DEGs) were identified in the roots of CCRI41/CCRI41 and SCRC22/CCRI41 in response to K deficiency, respectively. The overlapped and similarly (both up- or both down-) regulated DEGs in the two grafts were considered the basic response to K deficiency in cotton roots, whereas the DEGs only found in SCRC22/CCRI41 (1954) and those oppositely (one up- and the other down-) regulated in the two grafts might be the key factors involved in the feedback regulation of K uptake and root growth. The expression level of four putative K transporter genes (three and one ) increased in both grafts under low K, which could enable plants to cope with K deficiency. In addition, two ethylene response factors (ERFs), and , both down-regulated in the roots of CCRI41/CCRI41 and SCRC22/CCRI41, may negatively regulate K uptake in cotton roots due to higher net K uptake rate in their virus-induced gene silencing (VIGS) plants. In terms of feedback regulation of K uptake and root growth, several up-regulated DEGs related to Ca binding and CIPK (CBL-interacting protein kinases), one up-regulated and several up-regulated probably play important roles. In conclusion, these results provide a deeper insight into the molecular mechanisms involved in basic response to low K stress in cotton roots and feedback regulation of K uptake, and present several low K tolerance-associated genes that need to be further identified and characterized.
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http://dx.doi.org/10.3390/ijms22063133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003395PMC
March 2021

Miniature Seed6, encoding an endoplasmic reticulum signal peptidase, is critical in seed development.

Plant Physiol 2021 04;185(3):985-1001

State Key Laboratory of Plant Physiology and Biochemistry and National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, P. R. China.

Endoplasmic reticulum (ER) type I signal peptidases (ER SPases I) are vital proteases that cleave signal peptides from secreted proteins. However, the specific function of ER SPase I in plants has not been genetically characterized, and the substrate is largely unknown. Here, we report the identification of a maize (Zea mays) miniature seed6 (mn6) mutant. The loss-of-function mn6 mutant exhibited severely reduced endosperm size. Map-based cloning and molecular characterization indicated that Mn6 is an S26-family ER SPase I, with Gly102 (box E) in Mn6 critical for protein function during processing. Mass spectrometric and immunoprecipitation analyses revealed that Mn6 is predominantly involved in processing carbohydrate synthesis-related proteins, including the cell wall invertase miniature seed1 (Mn1), which is specifically expressed in the basal endosperm transfer layer. RNA and protein expression levels of Mn1 were both significantly downregulated in the mn6 mutant. Due to the significant reduction in cell wall invertase activity in the transfer cell layer, mutation of Mn6 caused dramatic defects in endosperm development. These results suggest that proper maturation of Mn1 by Mn6 may be a crucial step for proper seed filling and maize development.
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http://dx.doi.org/10.1093/plphys/kiaa060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133640PMC
April 2021

Erasing iatrogenic neoantigens from in vivo CRISPR screens.

Immunity 2021 Mar;54(3):406-408

Human Oncology and Pathogenesis Program, Immuno-Oncology Service, Memorial Sloan Kettering Cancer Center (MSKCC), New York, NY 10065, USA; Center for Cell Engineering, MSKCC, New York, NY 10065, USA; Parker Institute for Cancer Immunotherapy, New York, NY 10065, USA; Weill Cornell Medical College, New York, NY 10065, USA. Electronic address:

In vivo genetic screens using CRISPR-Cas9 are a powerful tool to resolve the molecular determinants of response and resistance to cancer immunotherapies; however, vector immunogenicity can introduce artifact. In this issue of Immunity, Dubrot et al. report a strategy to "erase" vector-associated neoantigens, enabling a more physiologic assessment of tumor-immune cell interactions in immunocompetent hosts.
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http://dx.doi.org/10.1016/j.immuni.2021.02.017DOI Listing
March 2021

The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.

Sci Adv 2021 Feb 24;7(9). Epub 2021 Feb 24.

Department of Pathology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35249-7331, USA.

Improper distribution of chromosomes during mitosis can contribute to malignant transformation. Higher eukaryotes have evolved a mitotic catastrophe mechanism for eliminating mitosis-incompetent cells; however, the signaling cascade and its epigenetic regulation are poorly understood. Our analyses of human cancerous tissue revealed that the NAD-dependent deacetylase SIRT2 is up-regulated in early-stage carcinomas of various organs. Mass spectrometry analysis revealed that SIRT2 interacts with and deacetylates the structural maintenance of chromosomes protein 1 (SMC1A), which then promotes SMC1A phosphorylation to properly drive mitosis. We have further demonstrated that inhibition of SIRT2 activity or continuously increasing SMC1A-K579 acetylation causes abnormal chromosome segregation, which, in turn, induces mitotic catastrophe in cancer cells and enhances their vulnerability to chemotherapeutic agents. These findings suggest that regulation of the SIRT2-SMC1A axis through deacetylation-phosphorylation permits escape from mitotic catastrophe, thus allowing early precursor lesions to overcome oncogenic stress.
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http://dx.doi.org/10.1126/sciadv.abe5518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904255PMC
February 2021

MP3RNA-seq: Massively parallel 3' end RNA sequencing for high-throughput gene expression profiling and genotyping.

J Integr Plant Biol 2021 Jul 7;63(7):1227-1239. Epub 2021 Apr 7.

State Key Laboratory of Plant Physiology and Biochemistry, National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.

Transcriptome deep sequencing (RNA-seq) has become a routine method for global gene expression profiling. However, its application to large-scale experiments remains limited by cost and labor constraints. Here we describe a massively parallel 3' end RNA-seq (MP3RNA-seq) method that introduces unique sample barcodes during reverse transcription to permit sample pooling immediately following this initial step. MP3RNA-seq allows for handling of hundreds of samples in a single experiment, at a cost of about $6 per sample for library construction and sequencing. MP3RNA-seq is effective for not only high-throughput gene expression profiling, but also genotyping. To demonstrate its utility, we applied MP3RNA-seq to 477 double haploid lines of maize. We identified 19,429 genes expressed in at least 50% of the lines and 35,836 high-quality single nucleotide polymorphisms for genotyping analysis. Armed with these data, we performed expression and agronomic trait quantitative trait locus (QTL) mapping and identified 25,797 expression QTLs for 15,335 genes and 21 QTLs for plant height, ear height, and relative ear height. We conclude that MP3RNA-seq is highly reproducible, accurate, and sensitive for high-throughput gene expression profiling and genotyping, and should be generally applicable to most eukaryotic species.
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http://dx.doi.org/10.1111/jipb.13077DOI Listing
July 2021

The Regulatory Effect of SIRT1 on Extracellular Microenvironment Remodeling.

Int J Biol Sci 2021 1;17(1):89-96. Epub 2021 Jan 1.

College of Basic Medical Science, Institute of Translational Medicine, Key Laboratory of Medical Cell Biology, Ministry of Education, Key Laboratory of Liaoning Province, China Medical University, Shenyang, Liaoning Province, P.R. China, 110122.

The sirtuins family is well known by its unique nicotinamide adenine dinucleotide (NAD)-dependent deacetylase function. The most-investigated member of the family, Sirtuin 1 (SIRT1), accounts for deacetylating a broad range of transcription factors and coregulators, such as p53, the Forkhead box O (FOXO), and so on. It serves as a pivotal regulator in various intracellular biological processes, including energy metabolism, DNA damage response, genome stability maintenance and tumorigenesis. Although the most attention has been focused on its intracellular functions, the regulatory effect on extracellular microenvironment remodeling of SIRT1 has been recognized by researchers recently. SIRT1 can regulate cell secretion process and participate in glucose metabolism, neuroendocrine function, inflammation and tumorigenesis. Here, we review the advances in the understanding of SIRT1 on remodeling the extracellular microenvironment, which may provide new ideas for pathogenesis investigation and guidance for clinical treatment.
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http://dx.doi.org/10.7150/ijbs.52619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757024PMC
January 2021

Corrigendum to "Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells".

Evid Based Complement Alternat Med 2020 8;2020:7939705. Epub 2020 Oct 8.

Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun 130041, China.

[This corrects the article DOI: 10.1155/2012/957568.].
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http://dx.doi.org/10.1155/2020/7939705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732371PMC
October 2020

Geographic population genetic structure and diversity of based on genotyping-by-sequencing (GBS).

PeerJ 2020 6;8:e9609. Epub 2020 Aug 6.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.

is a perennial leguminous low shrub endemic to the Yarlung Zangbo River basin in Tibet with irreplaceable economic and ecological value. To determine the drivers of evolution in this species, 225 individuals belonging to 15 populations from different geographic locations were sampled, and population genetics was studied using high-throughput genotyping-by-sequencing (GBS). Based on genetic diversity analysis, phylogenetic analysis, principal component analysis, and structure analysis, 15 natural populations were clustered into the following five subgroups: subgroup I (Shigatse subgroup) was located in the upper reaches of the Yarlung Zangbo River with a relatively high level of population genetic variation (means for PIC, Shannon and PI were 0.173, 0.326 and 0.0000305, respectively), and gene flow within the subgroup was also high (mean value for was 4.67). Subgroup II (including Pop 7 and Pop 8; means for PIC, Shannon and PI were 0.182, 0.345 and 0.0000321, respectively), located in the middle reaches of the Yarlung Zangbo River had relatively high levels of gene flow with the populations distributed in the upper and lower reaches. The between subgroup II with subgroups I and III was 3.271 and 2.894, respectively. Considering all the genetic diversity indices Pop 8 had relatively high genetic diversity. Subgroup III (the remaining mixed subgroup of Lhasa and Shannan) was located in the middle reaches of the Yarlung Zangbo River and the means for PIC, Shannon and PI were 0.172, 0.324 and 0.0000303, respectively. Subgroup IV (Nyingchi subgroup), located in the lower reaches of the Yarlung Zangbo River basin, showed a further genetic distance from the other subgroups and the means for PIC, Shannon and PI were 0.147, 0.277 and 0.0000263, respectively. Subgroup V (Nyingchi Gongbu Jiangda subgroup), located in the upper reaches of the Niyang River, had the lowest level of genetic variation (means for PIC, Shannon and PI were 0.106, 0.198 and 0.0000187, respectively) and gene flow with other populations (mean value for was 0.42). According to the comprehensive analysis, the populations generally expanded from upstream to downstream and displayed a high level of genetic differentiation in the populations in the upper and lower reaches. There were high levels of gene exchange between the central populations with upstream and downstream populations, and wind-induced seed dispersal was an important factor in the formation of this gene exchange mode.
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http://dx.doi.org/10.7717/peerj.9609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676378PMC
August 2020

Non-dispersive infrared multi-gas sensing via nanoantenna integrated narrowband detectors.

Nat Commun 2020 Oct 16;11(1):5245. Epub 2020 Oct 16.

School of Optical and Electronic Information and Wuhan National Research Center for Optoelectronics (WNLO), Huazhong University of Science and Technology, Wuhan, 430074, China.

Non-dispersive infrared (NDIR) spectroscopy analyzes the concentration of target gases based on their characteristic infrared absorption. In conventional NDIR gas sensors, an infrared detector has to pair with a bandpass filter to select the target gas. However, multiplexed NDIR gas sensing requires multiple pairs of bandpass filters and detectors, which makes the sensor bulky and expensive. Here, we propose a multiplexed NDIR gas sensing platform consisting of a narrowband infrared detector array as read-out. By integrating plasmonic metamaterial absorbers with pyroelectric detectors at the pixel level, the detectors exhibit spectrally tunable and narrowband photoresponses, circumventing the need for separate bandpass filter arrays. We demonstrate the sensing of HS, CH, CO, CO, NO, CHO, NO, SO. The detection limits of common gases such as CH, CO, and CO are 63 ppm, 2 ppm, and 11 ppm, respectively. We also demonstrate the deduction of the concentrations of two target gases in a mixture.
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http://dx.doi.org/10.1038/s41467-020-19085-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567865PMC
October 2020

The NAD-dependent deacetylase SIRT2 regulates T cell differentiation involved in tumor immune response.

Int J Biol Sci 2020 3;16(15):3075-3084. Epub 2020 Oct 3.

Institute of Translational Medicine, Key Laboratory of Cell Biology of Ministry of Public Health, and Key Laboratory of Medical Cell Biology of Ministry of Education, Liaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis, Treatment and Prevention, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang, 110042, Liaoning, China.

Sirtuin 2 (SIRT2), an NAD+-dependent deacetylase, regulates multiple biologic and pathologic processes including mitosis, genomic integrity, cell homeostasis and tumorigenesis. However, the role of SIRT2 in the immune response to cancer remains largely elusive. In this study, we found significantly lower expression of SIRT2 in peripheral T lymphocytes from breast cancer patients when compared to normal individuals. Moreover, SIRT2 levels positively correlated with CD8 effector memory T (T) cells in breast cancer patients. In keeping with these findings, altered T cells differentiation manifested as decreased T cells and increased naive T cells were observed in deficient mice. The upregulation of CD8 T by SIRT2 might attribute to the activation of aerobic oxidation as well as the inhibition of GSK3β acetylation in CD8 T cells. Taken together, these results suggest that SIRT2 participate in tumor immune response by regulating T cell differentiation, which may provide novel insight for tumor prevention and immune therapy.
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http://dx.doi.org/10.7150/ijbs.49735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545715PMC
October 2020

Genome-wide analysis of lncRNA and mRNA expression and endogenous hormone regulation during tension wood formation in Catalpa bungei.

BMC Genomics 2020 Sep 5;21(1):609. Epub 2020 Sep 5.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, PR China.

Background: Phytohormones are the key factors regulating vascular development in plants, and they are also involved in tension wood (TW) formation. Although the theory of hormone distribution in TW formation is widely supported, the effects of endogenous hormones on TW formation have not yet been assessed. In this study, TW formation was induced in Catalpa bungei by artificial bending. The phytohormone content of TW, opposite wood (OW) and normal wood (NW) was determined using liquid chromatography-mass spectrometry (LC-MS), and transcriptome sequencing was performed. The hormone content and related gene expression data were comprehensively analyzed.

Results: The results of analyses of the plant hormone contents indicated significantly higher levels of cis-zeatin (cZ), indoleacetic acid (IAA) and abscisic acid (ABA) in TW than in OW. Genes involved in the IAA and ABA synthesis pathways, such as ALDH (evm.

Model: group5.1511) and UGT (evm.

Model: scaffold36.20), were significantly upregulated in TW. and the expression levels of ARF (evm.

Model: group5.1332), A-ARR (evm.

Model: group0.1600), and TCH4 (evm.

Model: group2.745), which participate in IAA, cZ and Brassinolide (BR) signal transduction, were significantly increased in TW. In particular, ARF expression may be regulated by long noncoding RNAs (lncRNAs) and the HD-ZIP transcription factor ATHB-15.

Conclusions: We constructed a multiple hormone-mediated network of C. bungei TW formation based on hormone levels and transcriptional expression profiles were identified during TW formation.
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http://dx.doi.org/10.1186/s12864-020-07044-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487903PMC
September 2020

Genetic optimization of plasmonic metamaterial absorber towards dual-band infrared imaging polarimetry.

Opt Express 2020 Jul;28(15):22617-22629

Mid-infrared imaging detectors are essential tools for many applications because they can visualize the objects in the dark via thermal radiation. However, these detectors have to pair with separate spectral and polarization filters to select the target spectral bands and polarization states, resulting in complicated and bulky imaging systems. One way to mitigate the need for separate spectral filters and polarizers is to use metamaterial absorbers, which are arrays of optical resonators with sub-wavelength dimensions and spacing, to tailor the responses of the detector pixels. Here we report an intelligent program based on the genetic algorithm that automates the design and optimization of a metal-insulator-metal based metamaterial absorber with multi-sized nanostrip antennas as the top layer. The program starts from a randomly generated pattern of the top antenna layer, and it iteratively approaches the optimized designs of two polarization selective MIM absorbers with wideband high absorption in the specified 3-5 (MWIR) band and 8-12 µm (LWIR) band. The measured absorption spectra of the two optimized designs agree well with the simulated results. The influences of the incident angle of light, the finite size of detector pixels, and the air gap between the neighboring pixels on the spectral absorption are numerically evaluated.
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http://dx.doi.org/10.1364/OE.397868DOI Listing
July 2020

Efficacy and safety of cinepazide maleate injection in patients with acute ischemic stroke: a multicenter, randomized, double-blind, placebo-controlled trial.

BMC Neurol 2020 Jul 14;20(1):282. Epub 2020 Jul 14.

Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, 100730, China.

Background: Ischemic stroke is a leading cause of morbidity and mortality. Thrombolytic therapy improves disability and survival rates; however, to be effective, it must be given within 4.5 h of onset. Moreover, thrombolytic therapy is frequently contraindicated. Therefore, alternative therapeutic options are required. In China, cinepazide maleate injection has been shown to improve the cerebral collateral circulation and further reduce disability in stroke patients; however, very few studies investigating this therapy have been conducted to date. Therefore, this study aimed to further confirm the efficacy and safety of cinepazide maleate injection in patients with acute ischemic stroke.

Methods: Patients with acute ischemic stroke were administered an intravenous infusion of 320 mg cinepazide maleate or placebo once daily for 14 days. All patients were also administered basic therapy (citicoline sodium). The primary efficacy endpoint was the proportion of patients with a modified Rankin scale (mRS) ≤2 on day 90. Secondary efficacy endpoints included Barthel Index ≥95. Safety was evaluated by recording all adverse events (AEs), monitoring laboratory parameters and vital signs, and electrocardiogram.

Results: In total, 937 patients with an acute ischemic stroke were included, with a mean (standard deviation, SD) National Institutes of Health Stroke Scale score of 8.8 (2.4) and a mean (SD) stroke onset of 30.9 (11.4) hours prior. Following treatment for 90 days, the proportion of patients with an mRS score ≤ 2 was significantly higher in the cinepazide maleate group than in the control group (60.9% vs. 50.1%; p = 0.0004). Moreover, the proportion of patients with a Barthel Index of ≥95 on day 90 was also significantly higher in the cinepazide maleate group than in the control group (53.4% vs. 46.7%; p = 0.0230). There were no statistically significant differences in safety parameters between the cinepazide maleate and control groups.

Conclusions: The results of this study show that cinepazide maleate injection is superior to placebo in improving neurological function and activities of daily living, reducing disability, and promoting functional recovery in patients with acute ischemic stroke. Cinepazide maleate injection was safe and well tolerated with no unexpected AEs reported.

Trial Registration: Chinese Clinical Trial Registry CTR20160292 and ChiCTR1900023827 . Retrospectively registered June 13, 2019.
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http://dx.doi.org/10.1186/s12883-020-01844-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359492PMC
July 2020

Identification of Transposable Elements in Conifer and Their Potential Application in Breeding.

Evol Bioinform Online 2020 15;16:1176934320930263. Epub 2020 Jun 15.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.

Transposable elements (TEs) are known to play a role in genome evolution, gene regulation, and epigenetics, representing potential tools for genetics research in and breeding of conifers. Recently, thanks to the development of high-throughput sequencing, more conifer genomes have been reported. Using bioinformatics tools, the TEs of 3 important conifers (, and ) were identified in our previous study, which provided a foundation for accelerating the use of TEs in conifer breeding and genetic study. Here, we review recent studies on the functional biology of TEs and discuss the potential applications for TEs in conifers.
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http://dx.doi.org/10.1177/1176934320930263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297469PMC
June 2020

RNF8 induces β-catenin-mediated c-Myc expression and promotes colon cancer proliferation.

Int J Biol Sci 2020 1;16(12):2051-2062. Epub 2020 May 1.

Department of Anorectal Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, RP China.

DNA damage signals transducer RING finger protein 8 (RNF8) is involved in maintaining genomic stability by facilitating the repair of DNA double-strand breaks (DSB) via ubiquitin signaling. By analyzing the TCGA database and colon cancer tissue microarrays, we found that the expression level of RNF8 was positively correlated with that of c-Myc in colon cancer, which were closely associated with poor survival of colon cancer patients. Furthermore, overexpressing and knocking down RNF8 increased and decreased the expression of c-Myc in colon cancer cells, respectively. In addition, RNF8 interacted with β-catenin and facilitated its nuclear translocation by conjugating K63 polyubiquitination on it. These observations suggested a de novo role of RNF8 in promoting the progression of colon cancer by inducing β-catenin-mediated c-Myc expression.
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http://dx.doi.org/10.7150/ijbs.44119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294952PMC
May 2020

Accumulation of prelamin A induces premature aging through mTOR overactivation.

FASEB J 2020 06 13;34(6):7905-7914. Epub 2020 Apr 13.

Institute of Translational Medicine, Key Laboratory of Cell Biology of Ministry of Public Health, and Key Laboratory of Medical Cell Biology of Ministry of Education, Liaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis and Treatment and Prevention, China Medical University, Shenyang, China.

Hutchinson-Gilford progeria syndrome (HGPS) arises when a truncated form of farnesylated prelamin A accumulates at the nuclear envelope, leading to misshapen nuclei. Previous studies of adult Zmpste24-deficient mice, a mouse model of progeria, have reported a metabolic response involving inhibition of the mTOR (mammalian target of rapamycin) kinase and activation of autophagy. However, exactly how mTOR or autophagy is involved in progeria remains unclear. Here, we investigate this question by crossing Zmpste24 mice with mice hypomorphic in mTOR (mTOR ), or mice heterozygous in autophagy-related gene 7 (Atg7 ). We find that accumulation of prelamin A induces premature aging through mTOR overactivation and impaired autophagy in newborn Zmpste24 mice. Zmpste24 mice with genetically reduced mTOR activity, but not heterozygosity in Atg7, show extended lifespan. Moreover, mTOR inhibition partially restores autophagy and S6K1 activity. We also show that progerin interacts with the Akt phosphatase to promote full activation of the Akt/mTOR signaling pathway. Finally, although we find that genetic reduction of mTOR postpones premature aging in Zmpste24 KO mice, frequent embryonic lethality occurs. Together, our findings show that over-activated mTOR contributes to premature aging in Zmpste24 mice, and suggest a potential strategy in treating HGPS patients with mTOR inhibitors.
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http://dx.doi.org/10.1096/fj.201903048RRDOI Listing
June 2020

ATM-CHK2-Beclin 1 axis promotes autophagy to maintain ROS homeostasis under oxidative stress.

EMBO J 2020 05 18;39(10):e103111. Epub 2020 Mar 18.

Institute of Translational Medicine, Key Laboratory of Cell Biology of Ministry of Public Health, Key Laboratory of Medical Cell Biology of Ministry of Education, Liaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis and Treatment and Prevention, China Medical University, Shenyang, China.

The homeostatic link between oxidative stress and autophagy plays an important role in cellular responses to a wide variety of physiological and pathological conditions. However, the regulatory pathway and outcomes remain incompletely understood. Here, we show that reactive oxygen species (ROS) function as signaling molecules that regulate autophagy through ataxia-telangiectasia mutated (ATM) and cell cycle checkpoint kinase 2 (CHK2), a DNA damage response (DDR) pathway activated during metabolic and hypoxic stress. We report that CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, thereby impairing Beclin 1-Bcl-2 autophagy-regulatory complex formation in a ROS-dependent fashion. We further demonstrate that CHK2-mediated autophagy has an unexpected role in reducing ROS levels via the removal of damaged mitochondria, which is required for cell survival under stress conditions. Finally, CHK2 mice display aggravated infarct phenotypes and reduced Beclin 1 p-Ser90/Ser93 in a cerebral stroke model, suggesting an in vivo role of CHK2-induced autophagy in cell survival. Taken together, these results indicate that the ROS-ATM-CHK2-Beclin 1-autophagy axis serves as a physiological adaptation pathway that protects cells exposed to pathological conditions from stress-induced tissue damage.
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http://dx.doi.org/10.15252/embj.2019103111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232007PMC
May 2020

Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of .

Int J Mol Sci 2020 Mar 1;21(5). Epub 2020 Mar 1.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

is an economically important tree with high-quality wood and highly valuable to the study of wood formation. In this work, the xylem microstructure of tension wood (TW) was observed, and we performed transcriptomics, proteomics and Raman spectroscopy of TW, opposite wood (OW) and normal wood (NW). The results showed that there was no obvious gelatinous layer (G-layer) in the TW of and that the secondary wall deposition in the TW was reduced compared with that in the OW and NW. We found that most of the differentially expressed mRNAs and proteins were involved in carbohydrate polysaccharide synthesis. Raman spectroscopy results indicated that the cellulose and pectin content and pectin methylation in the TW were lower than those in the OW and NW, and many genes and proteins involved in the metabolic pathways of cellulose and pectin, such as galacturonosyltransferase (), polygalacturonase (), endoglucanase () and β-glucosidase () genes, were significantly upregulated in TW. In addition, we found that the MYB2 transcription factor may regulate the pectin degradation genes and , and ARF, ERF, SBP and MYB1 may be the key transcription factors regulating the synthesis and decomposition of cellulose. In contrast to previous studies on TW with a G-layer, our results revealed a change in metabolism in TW without a G-layer, and we inferred that the change in the pectin type, esterification and cellulose characteristics in the TW of may contribute to high tensile stress. These results will enrich the understanding of the mechanism of TW formation.
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http://dx.doi.org/10.3390/ijms21051686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084593PMC
March 2020

MoS Nanosheets Sensitized with Quantum Dots for Room-Temperature Gas Sensors.

Nanomicro Lett 2020 Feb 19;12(1):59. Epub 2020 Feb 19.

School of Optical and Electronic Information, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, People's Republic of China.

The Internet of things for environment monitoring requires high performance with low power-consumption gas sensors which could be easily integrated into large-scale sensor network. While semiconductor gas sensors have many advantages such as excellent sensitivity and low cost, their application is limited by their high operating temperature. Two-dimensional (2D) layered materials, typically molybdenum disulfide (MoS) nanosheets, are emerging as promising gas-sensing materials candidates owing to their abundant edge sites and high in-plane carrier mobility. This work aims to overcome the sluggish and weak response as well as incomplete recovery of MoS gas sensors at room temperature by sensitizing MoS nanosheets with PbS quantum dots (QDs). The huge amount of surface dangling bonds of QDs enables them to be ideal receptors for gas molecules. The sensitized MoS gas sensor exhibited fast and recoverable response when operated at room temperature, and the limit of NO detection was estimated to be 94 ppb. The strategy of sensitizing 2D nanosheets with sensitive QD receptors may enhance receptor and transducer functions as well as the utility factor that determine the sensor performance, offering a powerful new degree of freedom to the surface and interface engineering of semiconductor gas sensors.
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http://dx.doi.org/10.1007/s40820-020-0394-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770826PMC
February 2020

Construction of a high-density genetic map and QTL mapping of leaf traits and plant growth in an interspecific F population of Catalpa bungei × Catalpa duclouxii Dode.

BMC Plant Biol 2019 Dec 30;19(1):596. Epub 2019 Dec 30.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People's Republic of China.

Background: Catalpa bungei is an important tree species used for timber in China and widely cultivated for economic and ornamental purposes. A high-density linkage map of C. bungei would be an efficient tool not only for identifying key quantitative trait loci (QTLs) that affect important traits, such as plant growth and leaf traits, but also for other genetic studies.

Results: Restriction site-associated DNA sequencing (RAD-seq) was used to identify molecular markers and construct a genetic map. Approximately 280.77 Gb of clean data were obtained after sequencing, and in total, 25,614,295 single nucleotide polymorphisms (SNPs) and 2,871,647 insertions-deletions (InDels) were initially identified in the genomes of 200 individuals of a C. bungei (7080) × Catalpa duclouxii (16-PJ-3) F population and their parents. Finally, 9072 SNP and 521 InDel markers that satisfied the requirements for constructing a genetic map were obtained. The integrated genetic map contained 9593 pleomorphic markers in 20 linkage groups and spanned 3151.63 cM, with an average distance between adjacent markers of 0.32 cM. Twenty QTLs for seven leaf traits and 13 QTLs for plant height at five successive time points were identified using our genetic map by inclusive composite interval mapping (ICIM). Q16-60 was identified as a QTL for five leaf traits, and three significant QTLs (Q9-1, Q18-66 and Q18-73) associated with plant growth were detected at least twice. Genome annotation suggested that a cyclin gene participates in leaf trait development, while the growth of C. bungei may be influenced by CDC48C and genes associated with phytohormone synthesis.

Conclusions: This is the first genetic map constructed in C. bungei and will be a useful tool for further genetic study, molecular marker-assisted breeding and genome assembly.
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http://dx.doi.org/10.1186/s12870-019-2207-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937828PMC
December 2019

Healthy aging delays the neural processing of face features relevant for behavior by 40 ms.

Hum Brain Mapp 2020 04 29;41(5):1212-1225. Epub 2019 Nov 29.

Institute of Neuroscience and Psychology, University of Glasgow, Glasgow, UK.

Fast and accurate face processing is critical for everyday social interactions, but it declines and becomes delayed with age, as measured by both neural and behavioral responses. Here, we addressed the critical challenge of understanding how aging changes neural information processing mechanisms to delay behavior. Young (20-36 years) and older (60-86 years) adults performed the basic social interaction task of detecting a face versus noise while we recorded their electroencephalogram (EEG). In each participant, using a new information theoretic framework we reconstructed the features supporting face detection behavior, and also where, when and how EEG activity represents them. We found that occipital-temporal pathway activity dynamically represents the eyes of the face images for behavior ~170 ms poststimulus, with a 40 ms delay in older adults that underlies their 200 ms behavioral deficit of slower reaction times. Our results therefore demonstrate how aging can change neural information processing mechanisms that underlie behavioral slow down.
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http://dx.doi.org/10.1002/hbm.24869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268067PMC
April 2020

Correction to: Colorectal Cancer Diagnostic Algorithm Based on Sub-Patch Weight Color Histogram in Combination of Improved Least Squares Support Vector Machine for Pathological Image.

J Med Syst 2019 Nov 12;43(12):333. Epub 2019 Nov 12.

Department of Radiological Intervention, Shanghai Sixth People's Hospital East Campus Affiliated to Shanghai University of Medicine & Health Science, Shanghai, 201306, China.

The original article unfortunately contained a mistake. The corresponding author's name should be corrected as "Yingsheng Cheng".
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http://dx.doi.org/10.1007/s10916-019-1449-4DOI Listing
November 2019

Dual Model Medical Invoices Recognition.

Sensors (Basel) 2019 Oct 10;19(20). Epub 2019 Oct 10.

School of Petroleum Engineering, China University of Petroleum, Beijing 102249, China.

Hospitals need to invest a lot of manpower to manually input the contents of medical invoices (nearly 300,000,000 medical invoices a year) into the medical system. In order to help the hospital save money and stabilize work efficiency, this paper designed a system to complete the complicated work using a Gaussian blur and smoothing-convolutional neural network combined with a recurrent neural network (GBS-CR) method. Gaussian blur and smoothing (GBS) is a novel preprocessing method that can fix the breakpoint font in medical invoices. The combination of convolutional neural network (CNN) and recurrent neural network (RNN) was used to raise the recognition rate of the breakpoint font in medical invoices. RNN was designed to be the semantic revision module. In the aspect of image preprocessing, Gaussian blur and smoothing were used to fix the breakpoint font. In the period of making the self-built dataset, a certain proportion of the breakpoint font (the font of breakpoint is 3, the original font is 7) was added, in this paper, so as to optimize the Alexnet-Adam-CNN (AA-CNN) model, which is more suitable for the recognition of the breakpoint font than the traditional CNN model. In terms of the identification methods, we not only adopted the optimized AA-CNN for identification, but also combined RNN to carry out the semantic revisions of the identified results of CNN, meanwhile further improving the recognition rate of the medical invoices. The experimental results show that compared with the state-of-art invoice recognition method, the method presented in this paper has an average increase of 10 to 15 percentage points in recognition rate.
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http://dx.doi.org/10.3390/s19204370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832594PMC
October 2019

Application of Percutaneous Osteoplasty in Treating Pelvic Bone Metastases: Efficacy and Safety.

Cardiovasc Intervent Radiol 2019 Dec 23;42(12):1738-1744. Epub 2019 Sep 23.

Department of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.

Background: Percutaneous vertebroplasty has been a good option to treat vertebral metastases. The pelvic bone is a common site of spread for many cancers. Using follow-up data for 126 patients, we evaluated the safety and efficacy of percutaneous osteoplasty (POP) to treat pelvic bone metastases.

Materials And Methods: In this retrospective study, 126 patients (mean age 57.45 ± 11.46 years old) with 178 lesions were treated using POP. The visual analog scale (VAS), Oswestry Disability Index (ODI), and the changes in the patient's use of painkillers were used to evaluate pain and quality of life before the procedure, and at 3 days and 1, 3, 6, 9, and 12 months after the procedure.

Results: Technical success was achieved in all patients. The mean VAS scores decreased significantly from 6.87 ± 1.33 before the procedure to 3.33 ± 1.94 by day 3 after the procedure (P < 0.05), 2.26 ± 1.59 at 1 month (P < 0.05), 1.89 ± 1.53 at 3 months (P < 0.05), 1.87 ± 1.46 at 6 months (P < 0.05), 1.90 ± 1.47 at 9 months (P < 0.05), and 1.49 ± 1.17 at 12 months (P < 0.05). The ODI also changed after the procedure, with significant differences between baseline scores and at each follow-up examination (P < 0.05). Pain relief was achieved in 118 patients (93.65%); however, pain relief was not obvious in seven patients (5.56%), and pain was aggravated in one patient (0.79%). Extraosseous cement leakage occurred in 35 patients (27.78%) without causing any clinical complications.

Conclusion: Percutaneous osteoplasty is a safe and effective choice for patients with painful osteolytic pelvic bone metastases. It can relieve pain, reduce disability, and improve function.

Level Of Evidence: Level 3b, retrospective study.
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http://dx.doi.org/10.1007/s00270-019-02320-8DOI Listing
December 2019

Colorectal Cancer Diagnostic Algorithm Based on Sub-Patch Weight Color Histogram in Combination of Improved Least Squares Support Vector Machine for Pathological Image.

J Med Syst 2019 Aug 14;43(9):306. Epub 2019 Aug 14.

Department of Radiological Intervention, Shanghai Sixth People's Hospital East Campus Affiliated to Shanghai University of Medicine & Health Science, Shanghai, 201306, China.

In order to improve the diagnostic accuracy of colon cancer, a novel classification algorithm based on sub-patch weight color histogram and improved SVM is proposed, which has good approximation ability for complex pathological image. Our proposed algorithm combines wavelet kernel SVM with color histogram to classify pathological image. Firstly, the pathological image is divided into non-overlapping sub-patches, and the features of sub-patch histogram are extracted. Then, the global and local features are fused by the sub-patch weighting algorithm. Then, the RelicfF based forward selection algorithm is used to integrate color features and texture features so as to enhance the characterization capabilities of the tumor cell. Finally, Morlet wavelet kernel-based least squares support vector machine method is adopted to enhance the generalization ability of the model for small sample with non-linear and high-dimensional pattern classification problems. Experimental results show that the proposed pathological diagnostic algorithm can gain higher accuracy compared with existing comparison algorithms.
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http://dx.doi.org/10.1007/s10916-019-1429-8DOI Listing
August 2019
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