Publications by authors named "Fei Ye"

975 Publications

Structural basis of ketamine action on human NMDA receptors.

Nature 2021 Jul 28. Epub 2021 Jul 28.

Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.

Ketamine is a non-competitive channel blocker of N-methyl-D-aspartate (NMDA) receptors. A single sub-anaesthetic dose of ketamine produces rapid (within hours) and long-lasting antidepressant effects in patients who are resistant to other antidepressants. Ketamine is a racemic mixture of S- and R-ketamine enantiomers, with S-ketamine isomer being the more active antidepressant. Here we describe the cryo-electron microscope structures of human GluN1-GluN2A and GluN1-GluN2B NMDA receptors in complex with S-ketamine, glycine and glutamate. Both electron density maps uncovered the binding pocket for S-ketamine in the central vestibule between the channel gate and selectivity filter. Molecular dynamics simulation showed that S-ketamine moves between two distinct locations within the binding pocket. Two amino acids-leucine 642 on GluN2A (homologous to leucine 643 on GluN2B) and asparagine 616 on GluN1-were identified as key residues that form hydrophobic and hydrogen-bond interactions with ketamine, and mutations at these residues reduced the potency of ketamine in blocking NMDA receptor channel activity. These findings show structurally how ketamine binds to and acts on human NMDA receptors, and pave the way for the future development of ketamine-based antidepressants.
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http://dx.doi.org/10.1038/s41586-021-03769-9DOI Listing
July 2021

LINC01133 hampers the development of gastric cancer through increasing SST via binding to microRNA-576-5p.

Epigenomics 2021 Jul 28. Epub 2021 Jul 28.

Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410000, PR China.

Our study aimed at investigating how LINC01133 functions in gastric cancer (GC) progression. Gain-of-function and loss-of-function approaches were applied to analyze the effects of LINC01133, microRNA-576-5p (miR-576-5p) and somatostatin (SST) on the biological behaviors of GC cells and in tumor-bearing nude mice. GC tissues and cells showed low expression of LINC01133, and LINC01133 overexpression decreased malignant phenotypes of GC cells. Moreover, LINC01133 upregulated SST through binding to miR-576-5p. Overexpressing miR-576-5p or suppressing SST reversed the functions of LINC01133 in biological potentials of GC cells and tumor growth. LINC01133 overexpression may inhibit GC development by downregulation of miR-576-5p and upregulation of SST, which suggests new therapeutic targets for GC.
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http://dx.doi.org/10.2217/epi-2020-0377DOI Listing
July 2021

N6-Methyladenosine RNA modification in cerebrospinal fluid as a novel potential diagnostic biomarker for progressive multiple sclerosis.

J Transl Med 2021 Jul 22;19(1):316. Epub 2021 Jul 22.

Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Progressive multiple sclerosis (PMS) is an uncommon and severe subtype of MS that worsens gradually and leads to irreversible disabilities in young adults. Currently, there are no applicable or reliable biomarkers to distinguish PMS from relapsing-remitting multiple sclerosis (RRMS). Previous studies have demonstrated that dysfunction of N6-methyladenosine (m6A) RNA modification is relevant to many neurological disorders. Thus, the aim of this study was to explore the diagnostic biomarkers for PMS based on m6A regulatory genes in the cerebrospinal fluid (CSF).

Methods: Gene expression matrices were downloaded from the ArrayExpress database. Then, we identified differentially expressed m6A regulatory genes between MS and non-MS patients. MS clusters were identified by consensus clustering analysis. Next, we analyzed the correlation between clusters and clinical characteristics. The random forest (RF) algorithm was applied to select key m6A-related genes. The support vector machine (SVM) was then used to construct a diagnostic gene signature. Receiver operating characteristic (ROC) curves were plotted to evaluate the accuracy of the diagnostic model. In addition, CSF samples from MS and non-MS patients were collected and used for external validation, as evaluated by an m6A RNA Methylation Quantification Kit and by real-time quantitative polymerase chain reaction.

Results: The 13 central m6A RNA methylation regulators were all upregulated in MS patients when compared with non-MS patients. Consensus clustering analysis identified two clusters, both of which were significantly associated with MS subtypes. Next, we divided 61 MS patients into a training set (n = 41) and a test set (n = 20). The RF algorithm identified eight feature genes, and the SVM method was successfully applied to construct a diagnostic model. ROC curves revealed good performance. Finally, the analysis of 11 CSF samples demonstrated that RRMS samples exhibited significantly higher levels of m6A RNA methylation and higher gene expression levels of m6A-related genes than PMS samples.

Conclusions: The dynamic modification of m6A RNA methylation is involved in the progression of MS and could potentially represent a novel CSF biomarker for diagnosing MS and distinguishing PMS from RRMS in the early stages of the disease.
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http://dx.doi.org/10.1186/s12967-021-02981-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296732PMC
July 2021

Fragmenlt Recombination Design, Synthesis, and Safener Activity of Novel Ester-Substituted Pyrazole Derivatives.

J Agric Food Chem 2021 Jul 26. Epub 2021 Jul 26.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.

Fenoxaprop--ethyl (FE), a type of acetyl-CoA carboxylase (ACCase) inhibitor, has been extensively applied to a variety of crop plants. It can cause damage to wheat () even resulting in the death of the crop. On the prerequisite of not reducing herbicidal efficiency on target weed species, herbicide safeners selectively protect crops from herbicide injury. Based on fragment splicing, a series of novel substituted pyrazole derivatives was designed to ultimately address the phytotoxicity to wheat caused by FE. The title compounds were synthesized in a one-pot way and characterized infrared spectroscopy, H nuclear magnetic resonance, C nuclear magnetic resonance, and high-resolution mass spectrometry. The bioactivity assay proved that the FE phytotoxicity to wheat could be reduced by most of the title compounds. The molecular docking model indicated that compound prevented fenoxaprop acid (FA) from reaching or acting with ACCase. The absorption, distribution, metabolism, excretion, and toxicity predictions demonstrated that compound exhibited superior pharmacokinetic properties to the commercialized safener mefenpyr-diethyl. The current work revealed that a series of newly substituted pyrazole derivatives presented strong herbicide safener activity in wheat. This may serve as a potential candidate structure to contribute to the further protection of wheat from herbicide injury.
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http://dx.doi.org/10.1021/acs.jafc.1c02221DOI Listing
July 2021

Phenoxypyridine derivatives containing natural product coumarins with allelopathy as novel and promising proporphyrin IX oxidase-inhibiting herbicides: Design, synthesis and biological activity study.

Pestic Biochem Physiol 2021 Aug 9;177:104897. Epub 2021 Jun 9.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address:

To seek novel and safe protoporphyrinogen oxidase (PPO, EC 1.3.3.4) inhibitors with excellent herbicidal activity. A series of novel phenoxypyridine derivatives containing natural product coumarins with allelopathy were designed and synthesized based on bioisosterism and active subunit combination in this research. Compounds W3.1 and W3.4, with the half-maximal inhibitory concentration (IC) value of 0.02653 mg/L and 0.01937 mg/L, respectively, displayed excellent herbicidal activity in greenhouse. Their herbicidal activity was similar to commercial herbicide oxyfluorfen (IC = 0.04943 mg/L). The best field inhibitory effect of compounds W3.1 and W3.4 recorded was at doses of 450 g ai/ha and 300 g ai/ha, respectively. Compound W3.4 had the best herbicidal activity among all the target compounds in this paper. Molecular docking analysis revealed that compounds W3.1 and W3.4 could form a hydrogen bonds with the amino acid AGR-98 and a π-π superposition with the amino acid PHE-398, respectively, which was similar to the oxyfluorfen. The crop selectivity tests results indicated that maize, cotton and soybean showed high tolerance to compound W3.4. Compound W3.4 reduced the C and C contents of wheat and rice, but had less effect on maize, cotton and soybean. Selectivity of compound W3.4 in maize, cotton and soybean were appeared to be due to reduced absorption of the herbicide compared to wheat and rice. Compound W3.4 deserves further attention as a candidate structure for new herbicides.
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http://dx.doi.org/10.1016/j.pestbp.2021.104897DOI Listing
August 2021

MCA-Net: Multi-feature coding and attention convolutional neural network for predicting lncRNA-disease association.

IEEE/ACM Trans Comput Biol Bioinform 2021 Jul 20;PP. Epub 2021 Jul 20.

With the advent of the era of big data, it is troublesome to accurately predict the associations between lncRNAs and diseases based on traditional biological experiments due to its time-consuming and subjective. In this paper, we propose a novel deep learning method for predicting lncRNA-disease associations using multi-feature coding and attention convolutional neural network (MCA-Net). We first calculate six similarity features to extract different types of lncRNA and disease feature information. Second, a multi-feature coding method is proposed to construct the feature vectors of lncRNA-disease association samples by integrating the six similarity features. Furthermore, an attention convolutional neural network is developed to identify lncRNA-disease associations under 10-fold cross-validation. Finally, we evaluate the performance of MCA-Net from different perspectives including the effects of the model parameters, distinct deep learning models, and the necessity of attention mechanism. We also compare MCA-Net with several state-of-the-art methods on three publicly available datasets, i.e., LncRNADisease, Lnc2Cancer, and LncRNADisease2.0. The results show that our MCA-Net outperforms the state-of-the-art methods on all three of datasets. Besides, case studies on breast cancer and lung cancer further verify that MCA-Net is effective and accurate for the lncRNA-disease association prediction.
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http://dx.doi.org/10.1109/TCBB.2021.3098126DOI Listing
July 2021

Hepatic Steatosis and Ectopic Fat Are Associated With Differences in Subcutaneous Adipose Tissue Gene Expression in People With HIV.

Hepatol Commun 2021 Jul 27;5(7):1224-1237. Epub 2021 Feb 27.

Tennessee Center for AIDS Research Vanderbilt University Medical Center Nashville TN USA.

Persons with human immunodeficiency virus (PWH) have subcutaneous adipose tissue (SAT) dysfunction related to antiretroviral therapy and direct viral effects, which may contribute to a higher risk of nonalcoholic fatty liver disease compared with human immunodeficiency virus-negative individuals. We assessed relationships between SAT expression of major adipocyte regulatory and lipid storage genes with hepatic and other ectopic lipid deposits in PWH. We enrolled 97 PWH on long-term antiretroviral therapy with suppressed plasma viremia and performed computed tomography measurements of liver attenuation, a measure of hepatic steatosis, skeletal muscle (SM) attenuation, and the volume of abdominal subcutaneous, visceral, and pericardial adipose tissue. Whole SAT gene expression was measured using the Nanostring platform, and relationships with computed tomography imaging and fasting lipids were assessed using multivariable linear regression and network mapping. The cohort had a mean age of 47 years, body mass index of 33.4 kg/m, and CD4 count of 492 cells/mm. Lower liver attenuation, a marker of greater steatosis, was associated with differences in SAT gene expression, including lower lipoprotein lipase and acyl-CoA dehydrogenase, and higher phospholipid transfer protein. Lower liver attenuation clustered with lower visceral adipose tissue (VAT) attenuation and greater VAT volume, pericardial fat volume and triglycerides, but no relationship was observed between liver attenuation and SAT volume, SM attenuation, or low-density lipoprotein. Liver attenuation was associated with altered SAT expression of genes regulating lipid metabolism and storage, suggesting that SAT dysfunction may contribute to nonalcoholic fatty liver disease in PWH. SAT gene-expression relationships were similar for VAT volume and attenuation, but not SM, indicating that ectopic lipid deposition may involve multiple pathways.
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http://dx.doi.org/10.1002/hep4.1695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279464PMC
July 2021

A simple and rapid fluorescent approach for Pb determination and application in water samples and living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 10;263:120168. Epub 2021 Jul 10.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

A novel selective fluorescent chemosensor, thiosemicarbazide-appended naphthalimide derivative (TND), has been designed and synthesized, which exhibited good selectivity and sensibility for Pb in CHCN:HO (1:1) solution. The probe TND showed obvious color changes under UV light of 365 nm and displayed turn-on fluorescence response with Pb added. The binding mode of TND with Pb was found to be 1:1 based on the Job's plot analysis. The detection limit of Pb was 4.7 nM, which is far below the allowable concentration determined by WHO in drinking water. Moreover, the fortified recoveries of Pb were from 100.54% to 113.68% in water samples. TND is also applied for fluorescence imaging of Pb in lysosomes of human stromal cell line (HSC). This study indicated that TND would be a potential sensor detecting Pb in real sample.
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http://dx.doi.org/10.1016/j.saa.2021.120168DOI Listing
July 2021

Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study.

Int J Nanomedicine 2021 6;16:4661-4674. Epub 2021 Jul 6.

Institute of Modern Physics, Chinese Academy of Sciences; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Lanzhou, Gansu Province, 730000, People's Republic of China.

Purpose: Gold nanoparticles (AuNPs) are widely studied as radiosensitizers, but their radiosensitization in carbon ion radiotherapy is unsatisfactory. There is a lack of in vivo data on the radiosensitization of AuNPs under carbon ion irradiation. This study focused on the radiosensitization effect of AuNPs in the mouse melanoma cell line B16-F10 in vitro and in vivo.

Materials And Methods: 11-mercaptoundecanoic acid (11-MUA)-coated gold (Au) nanoparticles (mAuNPs) formulations were prepared and characterized. To verify the radiosensitization effect of mAuNPs, hydroxyl radicals were generated in aqueous solution, and the detection of intracellular reactive oxygen species (ROS) and clone survival were carried out in vitro. The tumor growth rate (TGR) and survival of mice were analyzed to verify the radiosensitization effect of mAuNPs in vivo. The apoptosis of tumor cells was detected, and the expression of key proteins in the apoptosis pathway was verified by immunohistochemistry.

Results: The intracellular ROS level in B16-F10 cells was enhanced by mAuNPs under carbon ion irradiation. The sensitization rate of mAuNPs was 1.22 with a 10% cell survival rate. Compared with irradiation alone, the inhibitory effect of mAuNPs combined with carbon ion irradiation on tumor growth was 1.94-fold higher, the survival time of mice was prolonged by 1.75-fold, and the number of apoptotic cells was increased by 1.43-fold. The ratio of key proteins Bax and Bcl2 in the apoptosis pathway was up-regulated, and the expression of caspase-3, a key executor of the apoptosis pathway, was up-regulated.

Conclusion: In in vivo and in vitro experiments, mAuNPs showed radiosensitivity to carbon ion irradiation. The sensitization effect of mAuNPs on mice tumor may be achieved by activating the mitochondrial apoptosis pathway and increasing tumor tissue apoptosis. To our best knowledge, the present study is the first in vivo evidence for radiosensitization of mAuNPs in tumor-bearing mice exposed to carbon ion irradiation.
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http://dx.doi.org/10.2147/IJN.S313678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275145PMC
July 2021

Evaluation of Thyroid Nodule Malignant Neoplasms and Obesity Among Children and Young Adults.

JAMA Netw Open 2021 Jul 1;4(7):e2116369. Epub 2021 Jul 1.

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee.

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http://dx.doi.org/10.1001/jamanetworkopen.2021.16369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271420PMC
July 2021

Changing trends of deep neck infections in southern China: A review of 127 cases.

Sci Prog 2021 Apr-Jun;104(2):368504211028367

The Department of Otolaryngology in the Zhongshan City People's Hospital, Zhongshan Affiliated Hospital of Sun Yat-sen University, Zhongshan, Guangdong, P.R. China.

This study aimed to review our experience with the clinical characteristics and management of deep neck infections (DNIs) and determine the changing trends of their characteristics over time in southern China. Patients diagnosed with a DNI between January 2009 and December 2018 were screened retrospectively for their demographic characteristics, etiology of infection, site of infection, microbiology, treatment, and complications. In total, 127 patients were included: 41 (32.3%) were treated between 2009 and 2013 (group A), and 86 (67.7%) were treated between 2014 and 2018 (group B). The most common site of infection in group A was the parapharyngeal space (15 patients, 36.6%), while that in group B involved multiple spaces (36 patients, 41.9%). The leucocyte count (×10 cells/L) was 13.23 ± 4.19 in group A and 16.04 ± 4.33 in group B ( < 0.001). was the most common bacteria in both groups. The mean hospital stay was 21.46 ± 33.09 days in group A and 10.44 ± 6.19 days in group B. The rate of diabetes mellitus (DM) in group A was lower than that in group B (8/41 and 33/86, respectively;  = 0.034). Airway obstruction was the most common complication in both groups. DNIs are more likely to show multi-space involvement, affect more DM patients, and be associated with higher leucocyte counts over time. We infer that the duration from morbidity to admission and that from admission to operation play roles in the successful management of DNIs, possibly causing fewer complications, lower mortality rates, and shorter hospital stays. DM patients require increased attention.
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http://dx.doi.org/10.1177/00368504211028367DOI Listing
June 2021

Far from black and white: Role of race, health literacy, and socioeconomic factors in the presentation of acute diverticulitis.

Surgery 2021 Jun 25. Epub 2021 Jun 25.

Department of Surgery, Division of Colon & Rectal Surgery, Vanderbilt University Medical Center, Nashville, TN. Electronic address:

Background: Racial discrepancies in treatment and outcomes of acute diverticulitis have been observed, yet underlying factors are poorly understood. We aimed to identify racial inequalities in health literacy among patients hospitalized with acute diverticulitis and characterize factors associated with more severe presentation.

Methods: We performed retrospective cohort analysis of 947 Black or White patients admitted with acute diverticulitis at a quaternary referral center from January 2009 through September 2019. Health literacy was determined by the validated Brief Health Literacy Screening, and socioeconomic status was defined by area deprivation index, a composite of multiple neighborhood socioeconomic deprivation measures. The primary outcome was severity of disease presentation represented by systemic inflammatory response syndrome criteria; secondary outcomes included intensive care unit admission, length of stay, and invasive interventions.

Results: Among all study participants, 121 (12.8%) self-identified as Black. Overall, 140 (14.8%) patients had inadequate health literacy, and 495 (52.3%) had area deprivation index greater than the national median. There was no association between race or area deprivation index and health literacy. A total of 340 (35.9%) patients met criteria for systemic inflammatory response syndrome, and 88 (9.3%) underwent an intervention; median length of stay was 3.5 days. Race, health literacy, and area deprivation index were not significantly associated with outcomes (P > .05).

Conclusion: Among patients with acute diverticulitis, no difference in severity of presentation by race, health literacy, or area deprivation index was observed. These findings suggest that differences in presentation of acute diverticulitis may not be driven by these social factors. Future studies should include considerations of clinical characteristics of acute diverticulitis, such as the role of access and underuse of healthcare resources.
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http://dx.doi.org/10.1016/j.surg.2021.05.049DOI Listing
June 2021

Daphnetin inhibits spinal glial activation via Nrf2/HO-1/NF-κB signaling pathway and attenuates CFA-induced inflammatory pain.

Int Immunopharmacol 2021 Jun 26;98:107882. Epub 2021 Jun 26.

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

Daphnetin (7, 8-dihydroxycoumarin, DAPH), a coumarin derivative isolated from Daphne odora var., recently draws much more attention as a promising drug candidate to treat neuroinflammatory diseases due to its protective effects against neuroinflammation. However, itscontribution to chronic inflammatory pain is largely unknown. In the current work, we investigated the effects of DAPH in a murine model of inflammatory pain induced by complete Freund's adjuvant (CFA) and its possible underlying mechanisms. Our results showed that DAPH treatment significantly attenuated mechanical allodynia provoked by CFA. A profound inhibition of spinal glial activation, followed by attenuated expression levels of spinal pro-inflammatory cytokines, was observed in DAPH-treated inflammatory pain mice. Further study demonstrated that DAPH mediated negative regulation of spinal NF-κB pathway, as well as its preferential activation of Nrf2/HO-1 signaling pathway in inflammatory pain mice. This study, for the first time, indicated that DAPH might preventthe development of mechanical allodynia in mice with inflammatory pain. And more importantly, these data provide evidence for the potential application of DAPH in the treatment of chronic inflammatory pain.
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http://dx.doi.org/10.1016/j.intimp.2021.107882DOI Listing
June 2021

Lifelong Teacher-Student Network Learning.

Authors:
Fei Ye Adrian Bors

IEEE Trans Pattern Anal Mach Intell 2021 Jun 25;PP. Epub 2021 Jun 25.

A unique cognitive capability of humans consists in their ability to acquire new knowledge and skills from a sequence of experiences. Meanwhile, artificial intelligence systems are good at learning only the last task for which they are trained while using their ability to generalise from the given data. We propose a novel lifelong learning methodology by employing a Teacher-Student network framework. While the Student module is trained with a new given database, the Teacher module would remind the Student about the information learnt in the past. The Teacher, implemented by a Generative Adversarial Network (GAN), is trained to preserve and replay past knowledge corresponding to the probabilistic representations of previously learn databases. Meanwhile, the Student module is implemented by a Variational Autoencoder (VAE) which infers its latent variable representation from both the output of the Teacher module as well as from the latest available database. Moreover, the Student module is trained to capture both continuous and discrete underlying data representations across different domains. This framework is extended to deal with lifelong learning problems in three distinct artificial systems learning situations: supervised, semi-supervised and unsupervised.
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http://dx.doi.org/10.1109/TPAMI.2021.3092677DOI Listing
June 2021

Design, synthesis, and herbicidal activity of novel phenoxypyridine derivatives containing natural product coumarin.

Pest Manag Sci 2021 Jun 23. Epub 2021 Jun 23.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, China.

Background: In recent years, protoporphyrinogen oxidase (PPO, EC 1.3.3.4) inhibitors have been widely studied as important agricultural herbicides. Our research focused on the design and synthesis of novel PPO inhibitor herbicides, through linking of a diphenylether pyridine bioisostere structure to substituted coumarins, which aims to enhance environmental and crop safety while retaining high efficacy.

Results: A total of 21 compounds were synthesized via acylation reactions and all compounds were characterized using infrared, H NMR, C NMR, and high-resolution mass spectra. The respective configurations of compounds IV-6 and IV-12 were also confirmed using single crystal X-ray diffraction. The bioassay results showed that the title compounds displayed notable herbicidal activity, particularly compound IV-6 which displayed better herbicidal activity in greenhouse and field experiments, crop selectivity and safety for cotton and soybean compared with the commercial herbicide oxyfluorfen.

Conclusion: The work revealed that compound IV-6 deserves further attention as a candidate structure for a novel and safe herbicide.
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http://dx.doi.org/10.1002/ps.6523DOI Listing
June 2021

Ruthenium-Catalyzed Deuteration of Aromatic Carbonyl Compounds with a Catalytic Transient Directing Group.

Chemistry 2021 Jul 17;27(38):9720. Epub 2021 Jun 17.

Leibniz-Institut für Katalyse e. V., Rostock, Albert-Einstein-Straße 29a, 18059, Rostock, Germany.

Invited for the cover of this issue are Helfried Neumann, Matthias Beller and co-workers at the Leibniz-Institut für Katalyse e. V., Rostock and Hangzhou Normal University. The image depicts "the case of ketone deuteration" being solved by Sherlock Holmes. Read the full text of the article at 10.1002/chem.202100468.
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http://dx.doi.org/10.1002/chem.202101157DOI Listing
July 2021

The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation.

Stem Cell Res Ther 2021 06 10;12(1):342. Epub 2021 Jun 10.

Tumor Immunology and Gene Therapy Center, Third Affiliated Hospital of Second Military Medical University, Shanghai, 200438, China.

Background: The liver possesses a powerful regeneration ability, which is correlated with the stemness of hepatocytes in the portal vein (PV). However, the mechanism underlying the maintenance of hepatocyte stemness has not been elucidated. Here, we hypothesized that high levels of lipopolysaccharide from the portal vein might maintain the stemness of hepatocytes in the PV area.

Methods: First, we examined the location of hepatic stem cells and the concentration of lipopolysaccharide (LPS) in the portal vein and inferior vena cava. Then, we assessed the effect of LPS on stemness maintenance in mice by using antibiotics to eliminate LPS and knocking out the LPS receptor, TLR4. In vitro, the effect of LPS on the stemness of hepatocytes was investigated by colony and sphere formation assays and assessment of pluripotent and stem cell marker expression. Furthermore, we studied the mechanism by which LPS regulates the stemness of hepatocytes. Finally, we ligated the portal vein branch to further verify the effect of LPS.

Results: We found that a high level of LPS from the portal vein was correlated with the location of hepatic stem cells in the PV area, and elimination of LPS by antibiotics inhibited the expression of the stemness marker. LPS promoted colony and sphere formation and induced the upregulation of pluripotent and stem cell markers in AML12 cells. Furthermore, in the reprogramming medium, LPS facilitated the dedifferentiation of mature hepatocytes into hepatic progenitor-like cells, which exhibited a bipotent differentiation capacity in vivo and in vitro. Mechanistically, LPS bound TLR4 to regulate stemness of hepatocytes via the activation of YAP1 signaling, and blockade of YAP1 abolished the LPS-induced cell stemness and upregulation of pluripotent markers.

Conclusions: Our study implies a correlation between LPS/TLR4/YAP1 signaling and cell stemness, and LPS was shown to be involved in stemness maintenance of hepatocytes in the PV area. LPS might be used to induce the dedifferentiation of mature hepatocytes into progenitor-like cells for repair of liver injury.
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http://dx.doi.org/10.1186/s13287-021-02421-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193885PMC
June 2021

Oncostatin M promotes hepatic progenitor cell activation and hepatocarcinogenesis via macrophage-derived tumor necrosis factor-α.

Cancer Lett 2021 Oct 5;517:46-54. Epub 2021 Jun 5.

Tumor Immunology and Gene Therapy Center, Third Affiliated Hospital of Second Military Medical University, Shanghai, 200438, China. Electronic address:

Hepatocellular carcinoma (HCC) usually occurs at the late stage of chronic liver injury. Oncostatin M (OSM) is a tumor-associated cytokine highly expressed in cirrhosis and HCC patients; however, its role in hepatocarcinogenesis has not been clearly elucidated. In this study, we investigated the effect of OSM on HCC occurrence in a rat model of N-diethylnitrosamine-induced HCC. OSM overexpression significantly increased the number of tumor nodules and shortened the overall survival of the rats. Notably, OSM promoted HPC activation in vivo but did not directly regulate the proliferation of the HPC cell line in vitro. Further, OSM induced tumor necrosis factor-α (TNF-α) secretion and CD68 macrophage accumulation, which were positively correlated with HPC activation. Additionally, TNF-α or macrophage depletion inhibited the promoting effect of OSM on hepatocarcinogenesis and HPC activation. Furthermore, OSM expression in the peritumoral tissues of HCC was positively correlated with poor overall survival of patients. In conclusion, OSM plays an important role in hepatocarcinogenesis by regulating the liver inflammation environment. Hence, OSM could be used as a potential target for HCC prevention and therapy or as an indicator of HCC prognosis.
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http://dx.doi.org/10.1016/j.canlet.2021.05.039DOI Listing
October 2021

Corticomuscular coupling analysis based on improved LSTM and transfer entropy.

Neurosci Lett 2021 Jun 5:136012. Epub 2021 Jun 5.

Key Laboratory of Brain Machine Collaborative Intelligence of Zhejiang Province, Hangzhou 310018, China; School of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou 310018, China. Electronic address:

The study of functional corticomuscular coupling can reflect the interaction between the cerebral cortex and muscle tissue, thereby helping to understand how the brain controls muscle tissue and the effect of muscle movement on brain function. This study proposes a detection model of the coupling strength between the cortex and muscles. The detection model uses an adaptive selector to choose the optimal long short-term memory network, uses this network to extract the features of electroencephalography and electromyography, and finally transforms time characteristics into the frequency domain. The transfer entropy is used to represent the interaction intensity of signals in different frequency bands. Using this model, we analyze the coupling relationship between the cortex and muscles in the three movements of wrist flexion, wrist extension, and clench fist, and compare the model with traditional wavelet coherence analysis and deep canonical correlation analysis. The experimental results show that our model can not only express the bidirectional coupling relationship between different frequency bands but also suppress the possible false coupling that traditional methods may detect. Our research shows that the proposed model has great potential in medical rehabilitation, movement decoding, and other fields.
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http://dx.doi.org/10.1016/j.neulet.2021.136012DOI Listing
June 2021

A new strategy for constructing artificial light-harvesting systems: supramolecular self-assembly gels with AIE properties.

Soft Matter 2021 Jun;17(23):5666-5670

College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, People's Republic of China.

An artificial light-harvesting system (ALHS) has been designed and constructed based on supramolecular organogels made of a simple hydrazide-functionalized benzimidazole derivative (HB), as well as the fluorescent dye rhodamine B (RhB). RhB acted as a good acceptor to realize the energy-transfer process with good efficiency based on a HB/RhB assembly, which showed considerable fluorescence resonance energy transfer (FRET) efficiency of 53% for the energy transfer process. Remarkably, the obtained system showed superior color conversion abilities, converting blue light into orange light. By properly tuning the donor to acceptor ratio, bright orange light emission was achieved with a high fluorescence quantum yield of 35.5%. This system exhibited promise for applications relating to visible-light photo-transformation.
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http://dx.doi.org/10.1039/d1sm00528fDOI Listing
June 2021

Cut-off values of lesion and vessel quantitative flow ratio in de novo coronary lesion post-drug-coated balloon therapy predicting vessel restenosis at mid-term follow-up.

Chin Med J (Engl) 2021 Jun 4;134(12):1450-1456. Epub 2021 Jun 4.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China.

Background: Drug-coated balloons (DCBs) have emerged as potential alternatives to drug-eluting stents in specific lesion subsets for de novo coronary lesions. Quantitative flow ratio (QFR) is a method based on the three-dimensional quantitative coronary angiography and contrast flow velocity during coronary angiography (CAG), obviating the need for an invasive fractional flow reserve procedural. This study aimed to assess the serial angiographic changes of de novo lesions post-DCB therapy and further explore the cut-off values of lesion and vessel QFR, which predict vessel restenosis (diameter stenosis [DS] ≥50%) at mid-term follow-up.

Methods: The data of patients who underwent DCB therapy between January 2014 and December 2019 from the multicenter hospital were retrospectively collected for QFR analysis. From their QFR performances, which were analyzed by CAG images at follow-up, we divided them into two groups: group A, showing target vessel DS ≥50%, and group B, showing target vessel DS <50%. The median follow-up time was 287 days in group A and 227 days in group B. We compared the clinical characteristics, parameters during DCB therapy, and QFR performances, which were analyzed by CAG images between the two groups, in need to explore the cut-off value of lesion/vessel QFR which can predict vessel restenosis. Student's t test was used for the comparison of normally distributed continuous data, Mann-Whitney U test for the comparison of non-normally distributed continuous data, and receiver operating characteristic (ROC) curves for the evaluation of QFR performance which can predict vessel restenosis (DS ≥50%) at mid-term follow-up using the area under the curve (AUC).

Results: A total of 112 patients with 112 target vessels were enrolled in this study. Group A had 41 patients, while group B had 71. Vessel QFR and lesion QFR were lower in group A than in group B post-DCB therapy, and the cut-off values of lesion QFR and vessel QFR in the ROC analysis to predict target vessel DS ≥50% post-DCB therapy were 0.905 (AUC, 0.741 [95% confidence interval, CI: 0.645, 0.837]; sensitivity, 0.817; specificity, 0.561; P < 0.001) and 0.890 (AUC, 0.796 [95% CI: 0.709, 0.882]; sensitivity, 0.746; specificity, 0.780; P < 0.001).

Conclusions: The cut-off values of lesion QFR and vessel QFR can assist in predicting the angiographic changes post-DCB therapy. When lesion/vessel QFR values are <0.905/0.890 post-DCB therapy, a higher risk of vessel restenosis is potentially predicted at follow-up.
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http://dx.doi.org/10.1097/CM9.0000000000001577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213272PMC
June 2021

[F]FEDAC translocator protein positron emission tomography-computed tomography for early detection of mitochondrial dysfunction secondary to myocardial ischemia.

Ann Nucl Med 2021 Aug 3;35(8):927-936. Epub 2021 Jun 3.

Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.

Background: This study aimed to evaluate the biodistribution and kinetics of [F]FEDAC targeting the translocator protein TSPO in the myocardium, and to explore its use for the identification of mitochondrial dysfunction. We also assessed the feasibility of [18F]FEDAC for the early detection of mitochondrial dysfunction associated with myocardial ischemia (MI).

Methods: The radiochemical purity and stability of [F]FEDAC were analyzed by radio-high-performance liquid chromatography (radio-HPLC). Its biodistribution and kinetics were evaluated by dissection and dynamic imaging using micro-positron emission tomography-computed tomography (micro-PET-CT) in healthy mice. [F]FEDAC was also applied in an MI rat model and in sham-operated controls. Mitochondrial changes were observed by immunohistochemical staining and electron microscopy.

Results: Radioactivity levels (%ID/g) in the myocardium in normal mice, determined by [F]FEDAC, were 8.32 ± 0.80 at 5 min and 2.40 ± 0.10 at 60 min. PET showed significantly decreased uptake by injured cardiac tissue in MI rats, with maximal normal-to-ischemic uptake ratios of 10.47 ± 3.03 (1.5 min) and 3.92 ± 1.12 (27.5 min) (P = 0.025). Immunohistochemistry confirmed that TSPO expression was decreased in MI rats. Mitochondrial ultrastructure demonstrated significant swelling and permeability.

Conclusion: [F]FEDAC uptake is reduced in the injured myocardium, consistent with mitochondrial dysfunction. These results may provide new evidence to aid the early detection of mitochondrial dysfunction associated with myocardial ischemic injury.
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http://dx.doi.org/10.1007/s12149-021-01630-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285353PMC
August 2021

3,3-Difluoroallyl ammonium salts: highly versatile, stable and selective gem-difluoroallylation reagents.

Nat Commun 2021 05 31;12(1):3257. Epub 2021 May 31.

Leibniz-Institute for Catalysis, Albert-Einstein-Str. 29a, Rostock, 18059, Germany.

The selective synthesis of fluorinated organic molecules continues to be of major importance for the development of bioactive compounds (agrochemicals and pharmaceuticals) as well as unique materials. Among the established synthetic toolbox for incorporation of fluorine-containing units, efficient and general reagents for introducing -CF- groups have been largely neglected. Here, we present the synthesis of 3,3-difluoropropen-1-yl ammonium salts (DFPAs) as stable, and scalable gem-difluoromethylation reagents, which allow for the direct reaction with a wide range of fascinating nucleophiles. DFPAs smoothly react with N-, O-, S-, Se-, and C-nucleophiles under mild conditions without necessity of metal catalysts with exclusive regioselectivity. In this way, the presented reagents also permit the straightforward preparation of many analogues of existing pharmaceuticals.
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http://dx.doi.org/10.1038/s41467-021-23504-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167079PMC
May 2021

Development and Validation of a Nomogram to Predict the Individual Future Stroke Risk for Adult Patients With Moyamoya Disease: A Multicenter Retrospective Cohort Study in China.

Front Neurol 2021 13;12:669025. Epub 2021 May 13.

Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Studies exploring the predictive performance of major risk factors associated with future stroke events are insufficient, and a useful tool to predict individual risk is not available. Therefore, personalized advice for preventing future stroke in patients with moyamoya disease (MMD) cannot provide evidence-based recommendations. The aim of this study was to develop a novel nomogram with reliable validity to predict the individual risk of future stroke for adult MMD patients. This study included 450 patients from seven medical centers between January 2013 and December 2018. Follow-ups were performed via clinical visits and/or telephone interviews from initial discharge to December 2019. The cohort was randomly assigned to a training set (2/3, = 300) for nomogram development and a test set (1/3, = 150) for external validation. The Kaplan-Meier analyses and receiver operating characteristic (ROC) curves were applied to assess the clinical benefits of this nomogram. Diabetes mellitus, a family history of MMD, a past history of stroke or transient ischemic attack, clinical manifestation, and treatment were identified as major risk factors via the least absolute shrinkage and selection operator (LASSO) method. A nomogram including these predictors was established via a multivariate Cox regression model, which displayed excellent discrimination [Harrell's concordance index (C-index), 0.85; 95% confidence interval (CI): 0.75-0.96] and calibration. In the external validation, the nomogram was found to have good discrimination (C-index, 0.81; 95% CI: 0.68-0.94) and calibration. In the subgroup analysis, this predictive nomogram also showed great performance in both ischemic-type (C-index, 0.90; 95% CI: 0.77-1.00) and hemorrhagic-type MMD (C-index, 0.72; 95% CI: 0.61-0.83). Furthermore, the nomogram was shown to have potential in clinical practice through Kaplan-Meier analyses and ROC curves. We developed a novel nomogram incorporating several clinical characteristics with relatively good accuracy, which may have considerable potential for evaluating individual future stroke risk and providing useful management recommendations for adult patients with MMD in clinical practice.
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http://dx.doi.org/10.3389/fneur.2021.669025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155507PMC
May 2021

Bardoxolone and bardoxolone methyl, two Nrf2 activators in clinical trials, inhibit SARS-CoV-2 replication and its 3C-like protease.

Signal Transduct Target Ther 2021 05 29;6(1):212. Epub 2021 May 29.

BNLMS, Peking-Tsinghua Center for Life Sciences at College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

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http://dx.doi.org/10.1038/s41392-021-00628-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164054PMC
May 2021

Dynamic fingerprint of fractionalized excitations in single-crystalline CuZn(OH)FBr.

Nat Commun 2021 May 24;12(1):3048. Epub 2021 May 24.

Shenzhen Institute for Quantum Science and Engineering, and Department of Physics, Southern University of Science and Technology, Shenzhen, China.

Beyond the absence of long-range magnetic orders, the most prominent feature of the elusive quantum spin liquid (QSL) state is the existence of fractionalized spin excitations, i.e., spinons. When the system orders, the spin-wave excitation appears as the bound state of the spinon-antispinon pair. Although scarcely reported, a direct comparison between similar compounds illustrates the evolution from spinon to magnon. Here, we perform the Raman scattering on single crystals of two quantum kagome antiferromagnets, of which one is the kagome QSL candidate CuZn(OH)FBr, and another is an antiferromagnetically ordered compound EuCu(OH)Cl. In CuZn(OH)FBr, we identify a unique one spinon-antispinon pair component in the E magnetic Raman continuum, providing strong evidence for deconfined spinon excitations. In contrast, a sharp magnon peak emerges from the one-pair spinon continuum in the E magnetic Raman response once EuCu(OH)Cl undergoes the antiferromagnetic order transition. From the comparative Raman studies, we can regard the magnon mode as the spinon-antispinon bound state, and the spinon confinement drives the magnetic ordering.
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http://dx.doi.org/10.1038/s41467-021-23381-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144382PMC
May 2021

Comparing intraoperative parathyroid identification based on surgeon experience versus near infrared autofluorescence detection - A surgeon-blinded multi-centric study.

Am J Surg 2021 May 13. Epub 2021 May 13.

Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, 37235, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA. Electronic address:

Background: Near infrared autofluorescence (NIRAF) detection has previously demonstrated significant potential for real-time parathyroid gland identification. However, the performance of a NIRAF detection device - PTeye® - remains to be evaluated relative to a surgeon's own ability to identify parathyroid glands.

Methods: Patients eligible for thyroidectomy and/or parathyroidectomy were enrolled under 6 endocrine surgeons at 3 high-volume institutions. Participating surgeons were categorized based on years of experience. All surgeons were blinded to output of PTeye® when identifying tissues. The surgeon's performance for parathyroid discrimination was then compared with PTeye®. Histology served as gold standard for excised specimens, while expert surgeon's opinion was used to validate in-situ tissues.

Results: PTeye® achieved 92.7% accuracy across 167 patients recruited. Junior surgeons (<5 years of experience) were found to have lower confidence in parathyroid identification and higher tissue misclassification rate per specimen when compared to PTeye® and senior surgeons (>10 years of experience).

Conclusions: NIRAF detection with PTeye® can be a valuable intraoperative adjunct technology to aid in parathyroid identification for surgeons.
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http://dx.doi.org/10.1016/j.amjsurg.2021.05.001DOI Listing
May 2021

Pyroelectric Catalysis-Based "Nano-Lymphatic" Reduces Tumor Interstitial Pressure for Enhanced Penetration and Hydrodynamic Therapy.

ACS Nano 2021 06 21;15(6):10488-10501. Epub 2021 May 21.

State Key Laboratory of Metastable Materials Science and Technology, Applying Chemistry Key Lab of Hebei Province, Yanshan University, Qinhuangdao 066004, China.

Because of the deficiency of lymphatic reflux in the tumor, the retention of tumor interstitial fluid causes aggravation of the tumor interstitial pressure (TIP), which leads to unsatisfactory tumor penetration of nanomedicine. It is the main inducement of tumor recurrence and metastasis. Herein, we design a pyroelectric catalysis-based "Nano-lymphatic" to decrease the TIP for enhanced tumor penetration and treatments. It realizes photothermal therapy and decomposition of tumor interstitial fluid under NIR-II laser irradiation after reaching the tumor, which reduces the TIP for enhanced tumor penetration. Simultaneously, reactive oxygen species generated during the pyroelectric catalysis can further damage deep tumor stem cells. The results indicate that the "Nano-lymphatic" relieves 52% of TIP, leading to enhanced tumor penetration, which effectively inhibits the tumor proliferation (93.75%) and recurrence. Our finding presents a rational strategy to reduce TIP by pyroelectric catalysis for enhanced tumor penetration and improved treatments, which is of great significance for drug delivery.
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http://dx.doi.org/10.1021/acsnano.1c03048DOI Listing
June 2021

A novel risk score for the prediction of airway management in patients with deep neck space abscess: a multicenter retrospective cohort study.

J Intensive Care 2021 May 20;9(1):41. Epub 2021 May 20.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou, Guangdong, People's Republic of China.

Background: Airway management, including noninvasive endotracheal intubation or invasive tracheostomy, is an essential treatment strategy for patients with deep neck space abscess (DNSA) to reverse acute hypoxia, which aids in avoiding acute cerebral hypoxia and cardiac arrest. This study aimed to develop and validate a novel risk score to predict the need for airway management in patients with DNSA.

Methods: Patients with DNSA admitted to 9 hospitals in Guangdong Province between January 1, 2015, and December 31, 2020, were included. The cohort was divided into the training and validation cohorts. The risk score was developed using the least absolute shrinkage and selection operator (LASSO) and logistic regression models in the training cohort. The external validity and diagnostic ability were assessed in the validation cohort.

Results: A total of 440 DNSA patients were included, of which 363 (60 required airway management) entered into the training cohort and 77 (13 required airway management) entered into the validation cohort. The risk score included 7 independent predictors (p < 0.05): multispace involvement (odd ratio [OR] 6.42, 95% confidence interval [CI] 1.79-23.07, p < 0.001), gas formation (OR 4.95, 95% CI 2.04-12.00, p < 0.001), dyspnea (OR 10.35, 95% CI 3.47-30.89, p < 0.001), primary region of infection, neutrophil percentage (OR 1.10, 95% CI 1.02-1.18, p = 0.015), platelet count to lymphocyte count ratio (OR 1.01, 95% CI 1.00-1.01, p = 0.010), and albumin level (OR 0.86, 95% CI 0.80-0.92, p < 0.001). Internal validation showed good discrimination, with an area under the curve (AUC) of 0.951 (95% CI 0.924-0.971), and good calibration (Hosmer-Lemeshow [HL] test, p = 0.821). Application of the clinical risk score in the validation cohort also revealed good discrimination (AUC 0.947, 95% CI 0.871-0.985) and calibration (HL test, p = 0.618). Decision curve analyses in both cohorts demonstrated that patients could benefit from this risk score. The score has been transformed into an online calculator that is freely available to the public.

Conclusions: The risk score may help predict a patient's risk of requiring airway management, thus advancing patient safety and supporting appropriate treatment.
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http://dx.doi.org/10.1186/s40560-021-00554-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139013PMC
May 2021

Electrospun Polymer-Free Nanofibers Incorporating Hydroxypropyl-β-cyclodextrin/Difenoconazole via Supramolecular Assembly for Antifungal Activity.

J Agric Food Chem 2021 Jun 20;69(21):5871-5881. Epub 2021 May 20.

Department of Chemistry, Northeast Agricultural University, Harbin 150030, China.

In this study, flexible and self-standing hydroxypropyl-β-cyclodextrin/difenoconazole inclusion complex (HPβCD/DZ-IC) nanofibers were prepared by polymer-free electrospinning, which exhibited potential to be a new fast-dissolving pesticide formulation. Scanning electron microscopy and optical microscopy were applied to evaluate the morphology of nanofibers, which showed that the resulting HPβCD/DZ-IC nanofibers were bead-free and uniform. In addition, the proton nuclear magnetic resonance (H NMR) spectrum suggested a stoichiometric ratio of 1:0.9 (HPβCD/DZ). Other characterization methods, such as UV-vis absorption, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA), were applied in this study. On the one hand, UV-vis absorption, fluorescence spectroscopy, FT-IR, XRD, and TGA provided useful information for the successful formation of an inclusion complex; on the other hand, the results of TGA indicated the thermal stability of DZ was enhanced after the formation of inclusion complexes. Besides, the phase solubility test could explain the increased water solubility of the nanofibers of inclusion complexes formed by DZ and HPβCD. The results of molecular docking studies demonstrated the most favorable binding interactions when HPβCD combined with DZ. The dissolution test and the antifungal performance test exhibited the characteristics of fast dissolution and the excellent antifungal performance of HPβCD/DZ-IC nanofibers, respectively.
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http://dx.doi.org/10.1021/acs.jafc.1c01351DOI Listing
June 2021
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