Publications by authors named "Fei Yan"

594 Publications

Acute and short-term efficacy of sauna treatment on cardiovascular function: Ameta-analysis.

Eur J Cardiovasc Nurs 2021 Apr;20(2):96-105

Laboratory of Biomechanical Engineering, Department of Applied Mechanics, College of Architecture & Environment, Sichuan University, Chengdu, China.

Objective: The role of sauna bathing in cardiovascular function treatment has been increasingly explored, but insufficient attention has been paid to its efficacy. We performed a meta-analysis to provide more evidence for the efficacy of sauna treatment in cardiovascular nursing.

Methods: Sixteen peer-reviewed journal articles were screened to summarize the efficacy of the sauna on cardiovascular function. Both acute (0-30 min after the sauna) and short-term (2-4 weeks following the sauna treatment) efficacies were investigated.

Results: For pooled acute efficacy, body temperature and heart rate significantly (p < 0.001) grew by 0.94°C and 17.86 beats/min, respectively; reductions of 5.55 mmHg (p < 0.001) and 6.50 mmHg (p < 0.001) were also observed in systolic blood pressure and diastole blood pressure, respectively. For combined short-term efficacy, left ventricular ejection fraction (LVEF), 6-min walk distance, and flow-mediated dilation (p < 0.001) increased by 3.27%, 48.11 m, and 1.71%, respectively; greater amelioration in LVEF was observed in participants with lower LVEF. The proportion of patients with New York Heart Association class III and IV decreased by 10.9% and 12.2%, respectively. Systolic blood pressure, diastolic blood pressure, brain natriuretic peptide concentration, left ventricular end-diastolic dimension, cardiothoracic ratio, and left atrial dimension reduced by 5.26 mmHg (p < 0.001), 4.14 mmHg (p < 0.001), 116.66 pg/mL (p < 0.001), 2.79 mm (p < 0.001), 2.628% (p < 0.05), and 1.88 mm (p < 0.05), respectively, while the concentration of norepinephrine in the plasma remained unchanged.

Conclusion: Sauna treatment was found to play a positive role in improving cardiovascular function and physical activity levels, especially in patients with low cardiovascular function. These findings reveal that thermal intervention may be a promising means for cardiovascular nursing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1474515120944584DOI Listing
April 2021

A class of independently evolved transcriptional repressors in plant RNA viruses facilitates viral infection and vector feeding.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China;

Plant viruses employ diverse virulence strategies to achieve successful infection, but there are few known general strategies of viral pathogenicity and transmission used by widely different plant viruses. Here, we report a class of independently evolved virulence factors in different plant RNA viruses which possess active transcriptional repressor activity. Rice viruses in the genera , , and all have transcriptional repressors that interact in plants with the key components of jasmonic acid (JA) signaling, namely mediator subunit OsMED25, OsJAZ proteins, and OsMYC transcription factors. These transcriptional repressors can directly disassociate the OsMED25-OsMYC complex, inhibit the transcriptional activation of OsMYC, and then combine with OsJAZ proteins to cooperatively attenuate the JA pathway in a way that benefits viral infection. At the same time, these transcriptional repressors efficiently enhanced feeding by the virus insect vectors by repressing JA signaling. Our findings reveal a common strategy in unrelated plant viruses in which viral transcriptional repressors hijack and repress the JA pathway in favor of both viral pathogenicity and vector transmission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2016673118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980396PMC
March 2021

Influence of arch support heights on the internal foot mechanics of flatfoot during walking: A muscle-driven finite element analysis.

Comput Biol Med 2021 Mar 26;132:104355. Epub 2021 Mar 26.

Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China; The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, China. Electronic address:

Background: Different arch support heights of the customized foot orthosis could produce different effects on the internal biomechanics of the foot. However, quantitative evidence is scarce. Therefore, we aimed to investigate and quantify the influence of arch support heights on the internal foot biomechanics during walking stance.

Methods: We reconstructed a foot finite element model from a volunteer with flexible flatfoot. The model enabled a three-dimensional representation of the plantar fascia and its interactions with surrounding osteotendinous structures. The volunteer walked in foot orthosis with different arch heights (low, neutral, and high). Muscle forces during gaits were calculated by a multibody model and used to drive a foot finite element model. The foot contact pressures and plantar fascia strains in different regions were compared among the insole conditions at the first and second vertical ground reaction force (VGRF) peak and VGRF valley instants.

Results: The results indicated that peak foot pressures decreased in balanced standing and second VGRF as the arch support height increased. However, peak midfoot pressures increased during all simulated instants. Meanwhile, high arch support decreased the plantar fascia loading by 5%-15.4% in proximal regions but increased in the middle and distal regions.

Conclusion: Although arch support could generally decrease the plantar foot pressure and plantar fascia loading, the excessive arch height may induce high midfoot pressure and loadings at the central portion of the plantar fascia. The consideration of fascia-soft tissue interaction in modeling could improve the prediction of plantar fascia strains towards design optimization for orthoses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiomed.2021.104355DOI Listing
March 2021

Ataxia telangiectasia mutated inhibitor-loaded copper sulfide nanoparticles for low-temperature photothermal therapy of hepatocellular carcinoma.

Acta Biomater 2021 Mar 31. Epub 2021 Mar 31.

Department of Hepatobiliary and Pancreatic Surgery, the First Hospital, Jilin University, 71 Xinmin Street, Changchun 130021, China. Electronic address:

Hepatocellular carcinoma (HCC) is the most frequent type of primary liver cancer, and is ranked the sixth most common neoplasm and the third leading cause of cancer-related deaths. Photothermal therapy (PTT) for thermal ablation of local tumors has recently emerged as a therapeutic strategy. However, the relatively high temperature of over 50 °C may lead to unexpected heat-related damage to tumor-adjacent normal tissues. Herein, we designed and synthesized ataxia telangiectasia mutated (ATM) inhibitor loaded hollow-structured CuS NPs with surface modification with anti-TGF-β antibody (CuS-ATMi@TGF-β NPs). CuS-ATMi@TGF-β NPs are highly photo-stable, can release encapsulated drugs, and increase the temperature to an effective level in a near-infrared (NIR)-responsive manner. Moreover, CuS-ATMi@TGF-β NPs specifically target tumors and thereby significantly inhibit tumor growth on contribution to synergistic low-temperature PTT and chemotherapy. This system not only achieved low-temperature PTT but also resulted in reduced damage to normal tissues. Modification with anti-TGF-β antibody enhanced target specificity and immune activation. The combination of PTT and ATM inhibitor showed synergistic effects and significantly attenuated the growth of the HCC via down regulation of heat shock protein (HSP). CuS-ATMi@TGF-β NPs are a highly promising platform for targeted tumor ablation via hyperthermia-mediated tumor death with minimal damage to normal tissues at a low temperature. STATEMENT OF SIGNIFICANCE: We constructed ataxia telangiectasia mutated (ATM) inhibitor-loaded hollow-structured CuS NPs with surface modification with anti-TGF-β antibody (CuS-ATMi@TGF-β NPs). CuS-ATMi@TGF-β NPs not only achieved low-temperature photothermal therapy (PTT) but also resulted in reduced damage to normal tissues and sufficient biocompatibility. The modification with anti-TGF-β antibody enhanced targeted specificity, cell endocytosis, and immune activation. In addition, the combination of PTT and ATM inhibitor synergistically attenuated the growth of the HCC via downregulation of heat shock protein (HSP). This study provided proof-of-concept for the ATM inhibitor that mediated low-temperature PTT with a potential for future clinical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2021.03.051DOI Listing
March 2021

A transgene-encoded truncated human epidermal growth factor receptor for depletion of anti- B-cell maturation antigen CAR-T cells.

Cell Immunol 2021 May 14;363:104342. Epub 2021 Mar 14.

Department of Oncology, Xinqiao Hospital, The Army Medical Unviersity, Chongqing, China. Electronic address:

Background: Chimeric antigen receptor T cells (CAR-T) against B-cell maturation antigen (BCMA) has been used to treat multiple myeloma (MM). CAR-T cells co-expressing a truncated human EGFR (tEGFR) has been proposed for in vivo cell ablation.

Methods: We designed and tested a novel anti-BCMA CAR. We transduced T cells with retroviral vectors encoding CAR and tEGFR. The anti-BCMA-CAR-transduced T cells were evaluated for the functions including cytokine production, proliferation, cytotoxicity, and in vivo tumor eradication of BCMA. Cetuximab was used for in vivo cell ablation.

Results: The CAR-T cells could specifically recognize BCMA, and anti-BCMA CAR-T cells could exhibit interferon-γ and cytotoxicity specifically produced by BCMA and eradicate tumor in vivo. Cetuximab could mediate antibody-dependent cellular cytotoxicity and in vivo elimination.

Conclusions: We confirm that BCMA is a suitable target for CAR- T cells and tEGFR is a effective tool for cellular ablation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellimm.2021.104342DOI Listing
May 2021

Extremely high Q-factor terahertz metasurface using reconstructive coherent mode resonance.

Opt Express 2021 Mar;29(5):7015-7023

High Q-factor resonance has a pivotal role in wide applications for manipulating electromagnetic waves. However, high Q-factor resonance, especially in the terahertz (THz) regime, has been a challenge faced by plasmonic metamaterials due to the inherent ohmic and radiation losses. Here, we theoretically present a unique metasurface scheme to produce extremely high Q-factor Fano resonance of the reconstructive coherent mode in the THz regime. The THz metasurface is composed of periodically arranged vertical symmetric split ring resonators (SRRs), which can produce perfect reconstructive coherent coupling effect in the sense that dipole radiation is destructively suppressed. Under the polarized electric field perpendicular to SRR gap, the surface currents are out of phase for an individual SRR, leading to the cancellation of net dipole moment. The reconstructive coherent mode resonance can occur between each SRR and its neighboring SRRs, accompanied by destructive interference of the scattered fields of each SRR. This is due to the coupling between the localized resonance of individual particles and the Rayleigh anomaly of the array. The proposed metasurface can significantly suppress far-field radiation and perform an extremely high Q-factor beyond 10 level with large modulation depth in the THz region, which pushes the advancement of THz high Q-factor resonance. The extremely high Q-factor of reconstructive coherent mode is tunable by adjusting the geometry parameters. The design strategy is useful to develop ultra-sensitive sensors, narrow-band filters and strong interaction of field-matter in the THz regime.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.417367DOI Listing
March 2021

Safety and efficacy of rituximab in connective tissue disease-associated interstitial lung disease: A systematic review and meta-analysis.

Int Immunopharmacol 2021 Mar 12;95:107524. Epub 2021 Mar 12.

Department of Rheumatology, The 1st Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. Electronic address:

Objective: Rituximab (RTX) is widely used in the treatment of connective tissue disease (CTD) because it can target and eliminate pathogenic B cells. Interstitial lung disease (ILD) is one of the common complications of CTD; however, the clinical benefits of RTX in connective tissue disease-associated interstitial lung disease (CTD-ILD) are still controversial. This meta-analysis was performed to systematically evaluate the efficacy and safety of RTX in CTD-ILD patients.

Methods: We performed a systematic online query in PubMed, Cochrane, and Embase up to February 2020. Randomized controlled trials and observational studies on the use of RTX and conventional treatment in CTD-ILD patients were comprehensively reviewed and investigated.

Results: In total, 6 studies, including 242 participants, were analysed. The pooled results showed that RTX is superior to conventional treatment methods in improving forced vital capacity and modified Rodnan skin scores (MRSS) in patients with systemic sclerosis (P<0. 05), but there was no statistically significant difference between RTX and conventional treatment method in the improvement of lung diffusion function. The risk of adverse effects declined in the RTX therapy groups compared with the conventional therapy groups in terms of infection and the blood system.

Conclusion: The pooled results of this meta-analysis indicated that RTX is well tolerated, and RTX is able to improve or stabilize pulmonary function in CTD-ILD patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2021.107524DOI Listing
March 2021

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus.

Arch Virol 2021 May 8;166(5):1427-1431. Epub 2021 Mar 8.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. This cDNA clone was infectious by agro-infiltration, leading to systemic symptoms in Nicotiana benthamiana, tomato, pepper, and potato.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-05018-wDOI Listing
May 2021

Sensitization of nerve cells to ultrasound stimulation through Piezo1-targeted microbubbles.

Ultrason Sonochem 2021 Feb 13;73:105494. Epub 2021 Feb 13.

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address:

Neuromodulation by ultrasound (US) has recently drawn considerable attention due to its great advantages in noninvasiveness, high penetrability across the skull and highly focusable acoustic energy. However, the mechanisms and safety from US irradiation still remain less understood. Recently, documents revealed Piezo1, a mechanosensitive cation channel, plays key role in converting mechanical stimuli from US through its trimeric propeller-like structure. Here, we developed a Piezo1-targeted microbubble (PTMB) which can bind to the extracellular domains of Piezo1 channel. Due to the higher responsiveness of bubbles to mechanical stimuli from US, significantly lower US energy for these PTMB-binding cells may be needed to open these mechanosensitive channels. Our results showed US energy at 0.03 MPa of peak negative pressure can achieve an equivalent level of cytoplasmic Ca transients which generally needs 0.17 MPa US intensity for the control cells. Cytoplasmic Ca elevations were greatly reduced by chelating extracellular calcium ions or using the cationic ion channel inhibitors, confirming that US-mediated calcium influx are dependent on the Piezo1 channels. No bubble destruction and obvious temperature increase were observed during the US exposure, indicating cavitation and heating effects hardly participate in the process of Ca transients. In conclusion, our study provides a novel strategy to sensitize the response of nerve cells to US stimulation, which makes it safer application for US-mediated neuromodulation in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ultsonch.2021.105494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921623PMC
February 2021

"Sandwich" wound dressing to reduce surgical site infections during sacrococcygeal surgery: A retrospective analysis.

J Tissue Viability 2020 Aug 4. Epub 2020 Aug 4.

Department of Spinal Surgery, First Affiliated Hospital of Harbin Medical University, No.23 Youzheng Street, 150001, Harbin, China. Electronic address:

Objective: To explore whether the "sandwich" wound dressing method with a hydrocolloid dressing can effectively reduce surgical site infection rates in sacrococcygeal aseptic operations.

Methods: A retrospective cohort of patients with sacrococcygeal aseptic operations (class I incision category) from January 2017 to March 2018 were divided into intervention (sandwich dressing) and control groups (conventional dressing). The surgical site infections (SSI) rate, wound healing course, hospitalization time, and medical costs in the two groups were determined. To exclude the influence of other factors, operation time, blood loss, age, sex ratio, the distance of the incision from the distal edge to the anus, and initial defecation times were compared between the groups.

Results: The SSI rates and medical costs in the interventional group were significantly lower than the control group (0% vs 78.57%, P < 0.0001; 3.27 ± 0.98 vs 5.83 ± 1.66 ¥10,000, p < 0.0001). Hospitalization times were also lower in the intervention compared to the control group (17.05 ± 4.77 vs 34.50 ± 15.47 day, P = 0.001).

Conclusions: The sandwich wound dressing method with a hydrocolloid dressing can effectively prevent SSI during sacrococcygeal aseptic surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtv.2020.07.008DOI Listing
August 2020

Complete genome sequence of passiflora virus Y infecting passion fruit in China.

Arch Virol 2021 May 26;166(5):1489-1493. Epub 2021 Feb 26.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

The complete genome sequence of passiflora virus Y (PaVY) from passion fruit growing in Guangdong province, China, was determined. The entire positive single-strand RNA genome comprises 9681 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3084 amino acids flanked by 5' and 3' untranslated regions of 169 and 257 nt, respectively. In sequence comparisons and phylogenetic analysis, PaVY appears to represent a new species in the bean common mosaic virus subgroup of the genus Potyvirus. This is the first report of the complete genome sequence of PaVY and the first report of this virus in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-05013-1DOI Listing
May 2021

Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X.

Mol Plant Pathol 2021 Apr 24;22(4):456-464. Epub 2021 Feb 24.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China.

The apoplast is the extracellular space for signalling, nutrient transport, and plant-microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)-infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription-PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high-speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one-way manner and do not share the bidirectional transport of exosomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/mpp.13039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938632PMC
April 2021

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China.

Virol J 2021 02 23;18(1):42. Epub 2021 Feb 23.

State Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo, 315211, Zhejiang, China.

Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5' half of its genome (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3'-part encoding P5, where it is most similar (91.7% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event affecting nucleotide positions 4126 to 5192 nt, with PeVYV-3 as the major parent but with the region 4126-5192 nt derived from TVDV as the minor parent. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12985-021-01511-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901092PMC
February 2021

"Hand As Foot" teaching method in central vein catheterization: A neonate teaching experience.

Authors:
Fei Yan Li Xu

Asian J Surg 2021 Apr 19;44(4):676-677. Epub 2021 Feb 19.

Inner Mongolia Medical University, Department of Neonatology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010050, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.asjsur.2021.01.033DOI Listing
April 2021

Cerebrovascular segmentation from TOF-MRA based on multiple-U-net with focal loss function.

Comput Methods Programs Biomed 2021 Apr 11;202:105998. Epub 2021 Feb 11.

School of Computer and Communication Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Background And Objective: Accurate cerebrovascular segmentation plays an important role in the diagnosis of cerebrovascular diseases. Considering the complexity and uncertainty of doctors' manual segmentation of cerebral vessels, this paper proposed an automatic segmentation algorithm based on Multiple-U-net (M-U-net) to segment cerebral vessel structures from the Time-of-flight Magnetic Resonance Angiography (TOF-MRA) data.

Methods: First, the TOF-MRA data was normalized by volume and then divided into three groups through slices of axial, coronal and sagittal directions respectively. Three single U-nets were trained by divided dataset. To solve the problem of uneven distribution of positive and negative samples, the focal loss function was adopted in training. After obtaining the prediction results of three single U-nets, the voting feature fusion and the post-processing process based on connected domain analysis would be performed. 95 volumes of TOF-MRA provided by the MIDAS platform were applied to the experiment, among which 20 volumes were treated as the training dataset, 5 volumes were used as the validation dataset and the remaining 70 volumes were divided into 10 groups to test the trained model respectively.

Results: Experiments showed that the proposed M-U-net based algorithm achieved 88.60% and 87.93% Dice Similarity Coefficient (DSC) on the verification dataset and testing dataset, which performed better than any single U-net.

Conclusions: Compared with other existing algorithms, our algorithm reached the state of the art level. The feature fusion of three single U-nets could effectively complement the segmentation results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cmpb.2021.105998DOI Listing
April 2021

Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles.

Biomater Sci 2021 Apr 17;9(7):2454-2466. Epub 2021 Feb 17.

Shenzhen Medical Ultrasound Engineering Center, Department of Ultrasonography, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, First Clinical Medical College of Southern University of Science and Technology, Shenzhen 518020, China.

The success of gene therapy depends largely on the development of gene vectors and effective gene delivery systems. It has been demonstrated that cationic microbubbles can be loaded with negatively charged plasmid DNA and thus improve gene transfection efficiency. In this study, we developed dual-targeting cationic microbubbles conjugated with iRGD peptides(Cyclo(Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys)) and CCR2 (chemokine (C-C motif) receptor 2) antibodies (MB) for ultrasound molecular imaging and targeted tumor gene therapy. The ultrasound molecular imaging experiments showed that there were significantly enhanced ultrasound molecular imaging signals in the tumor that received MB, compared with those that received MB, MB, or MB. As a therapy plasmid, pGPU6/GFP/Neo-shAKT2, carrying an expression cassette for the human AKT2 RNA interference sequence, was used. Our results demonstrated that MB had a significantly higher gene transfection efficiency than MB, MB, or MB under ultrasound irradiation, resulting in much lower AKT2 protein expression and stronger tumor growth inhibition effects in vivo and in vitro. In conclusion, our study demonstrated a novel gene delivery system via MB for ultrasound molecular-imaging-guided gene therapy of breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0bm01857kDOI Listing
April 2021

Integration of vertically-ordered mesoporous silica-nanochannel film with electro-activated glassy carbon electrode for improved electroanalysis in complex samples.

Talanta 2021 Apr 29;225:122066. Epub 2020 Dec 29.

Department of Chemistry, Zhejiang Sci-Tech University, 928 Second Avenue, Xiasha Higher Education Zone, Hangzhou, 310018, PR China. Electronic address:

Vertically-ordered mesoporous silica-nanochannel films (VMSF) with highly ordered nanochannels, uniform and adjustable pore size, ultra-thin thickness, and high porosity, have attracted considerable attention in analysis, molecular separation, catalysis, and nanomaterial synthesis. However, their widespread applications in practical electrochemical sensing are largely limited by the poor adhesion to common electrode materials, especially the lack of highly active substrate electrode to equip mechanically stable VMSF. Herein, we report a facile strategy to fabricate VMSF on widely used sensing electrodes without the use of any chemical adhesive for developing superior VMSF based electrochemical sensors. We demonstrate that simple electrochemical polarization (anodic polarization and subsequent cathodic reduction) to activate glassy carbon electrode (GCE) can generate a suitable surface environment allowing direct growth of stable VMSF on such pre-activated GCE (p-GCE) via electrochemically assisted self-assembly (EASA). Compared to traditional VMSF electrodes with ITO or organosilane grafted GCE as substrate, the developed VMSF/p-GCE exhibits much higher electrochemical response to four redox biomarkers (norepinephrine, dopamine, tryptophan, and uric acid). In-depth insights on mechanisms of the high electrochemical activity and incorporation stability of VMSF/p-GCE are made. We further demonstrate the VMSF/p-GCE can be employed to detect dopamine in real serum samples with exceptional sensitivity, low detection potential, as well as superior anti-interference and anti-fouling performance. In addition, high selectivity is realized as the common co-existing interference substances (ascorbic acid-AA and uric acid-UA) do not interfere with the detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2020.122066DOI Listing
April 2021

Down-regulation of estrogen-related receptor alpha (ERRα) inhibits gastric cancer cell migration and invasion and .

Aging (Albany NY) 2021 02 11;13(4):5845-5857. Epub 2021 Feb 11.

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, Jiangsu Province, China.

Objective: To investigate the correlation between estrogen-related receptor a (ERRα) expression level and gastric cancer (GC).

Methods: We collected GC and adjacent normal tissues from 50 patients. The parameters of the patients were summarized, and correlation with the expression level of ERRα was calculated. Downregulated ERRα using lentivirus was designed and transfected to SGC-7901 and MGC-803 cells. Cell migration, invasion and wound assays were conducted to determine the correlation between ERRα and capacity for cell migration and invasion. The expression level of the genes involved in epithelial-mesenchymal transition, including E-cadherin, γ-catenin, N-cadherin and vimentin, was determined via real-time or quantitative polymerase chain reaction(qPCR) and Western blot analysis.

Results: The expression of ERRα tends to be higher in GC tissues than in adjacent normal tissues. Analyses ofthe expression level of ERRα and patient parameters show that the ERRα level is significantly correlated with TNM staging and patient survival (<0.05). The downregulation of ERRα can inhibit cell invasion and migration, which was proven by Transwell and cell wound assays. The levels of E-cadherin and γ-catenin increased by conducting qPCR and Western blot analysis. Meanwhile, the levels of N-cadherin and vimentin decreased when ERRα expression was reduced.

Conclusion: ERRα is highly expressed in GC tissues and can promote the migration and invasion of cancer cells. It can be a potential marker for GC diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950300PMC
February 2021

Multi-level quantum noise spectroscopy.

Nat Commun 2021 Feb 11;12(1):967. Epub 2021 Feb 11.

Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.

System noise identification is crucial to the engineering of robust quantum systems. Although existing quantum noise spectroscopy (QNS) protocols measure an aggregate amount of noise affecting a quantum system, they generally cannot distinguish between the underlying processes that contribute to it. Here, we propose and experimentally validate a spin-locking-based QNS protocol that exploits the multi-level energy structure of a superconducting qubit to achieve two notable advances. First, our protocol extends the spectral range of weakly anharmonic qubit spectrometers beyond the present limitations set by their lack of strong anharmonicity. Second, the additional information gained from probing the higher-excited levels enables us to identify and distinguish contributions from different underlying noise mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21098-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878521PMC
February 2021

Exploring the mechanism of (-)-Epicatechin on premature ovarian insufficiency based on network pharmacology and experimental evaluation.

Biosci Rep 2021 Feb;41(2)

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Methods: Relevant potential targets for EC were obtained based on Traditional Chinese Medicine System Pharmacology Database (TCMSP), a bioinformatics analysis tool for molecular mechanism of Traditional Chinese Medicine (BATMAN-TCM) and STITCH databases. The Online Mendelian Inheritance in Man (OMIM) and GeneCards databases were utilized to screen the known POI-related targets, while Cytoscape software was used for network construction and visualization. Then, the Gene Ontology (GO) and pathway enrichment analysis were carried out by the Database for Annotation, Visualization and Integrated Discovery (DAVID) database. Furthermore, KGN cells were performed to validate the predicted results in oxidative stress (OS) model, and antioxidant effect was examined.

Results: A total of 70 potential common targets for EC in the treatment of POI were obtained through network pharmacology. Metabolic process, response to stimulus and antioxidant activity occupied a leading position of Gene Ontology (GO) enrichment. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that PI3K/protein kinase B (AKT), TNF, estrogen, VEGF and MAPK signaling pathways were significantly enriched. In addition, cell experiments showed that EC exhibited antioxidant effects in an H2O2-mediated OS model in ovarian granulosa cells by regulating the expression of PI3K/AKT/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway and multiple downstream antioxidant enzymes.

Conclusion: EC could regulate multiple signaling pathways and several biological processes (BPs). EC had the ability to down-regulate elevated OS level through the PI3K/AKT/Nrf2 signaling pathway and represented a potential novel treatment for POI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20203955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881164PMC
February 2021

A Robust Lane Detection Model Using Vertical Spatial Features and Contextual Driving Information.

Sensors (Basel) 2021 Jan 21;21(3). Epub 2021 Jan 21.

School of Information Science and Technology, Southwest Jiaotong University, Chengdu 611756, China.

The quality of detected lane lines has a great influence on the driving decisions of unmanned vehicles. However, during the process of unmanned vehicle driving, the changes in the driving scene cause much trouble for lane detection algorithms. The unclear and occluded lane lines cannot be clearly detected by most existing lane detection models in many complex driving scenes, such as crowded scene, poor light condition, etc. In view of this, we propose a robust lane detection model using vertical spatial features and contextual driving information in complex driving scenes. The more effective use of contextual information and vertical spatial features enables the proposed model more robust detect unclear and occluded lane lines by two designed blocks: feature merging block and information exchange block. The feature merging block can provide increased contextual information to pass to the subsequent network, which enables the network to learn more feature details to help detect unclear lane lines. The information exchange block is a novel block that combines the advantages of spatial convolution and dilated convolution to enhance the process of information transfer between pixels. The addition of spatial information allows the network to better detect occluded lane lines. Experimental results show that our proposed model can detect lane lines more robustly and precisely than state-of-the-art models in a variety of complex driving scenarios.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21030708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864510PMC
January 2021

Activation of Toll Immune Pathway in an Insect Vector Induced by a Plant Virus.

Front Immunol 2020 8;11:613957. Epub 2021 Jan 8.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.

The Toll pathway plays an important role in defense against infection of various pathogenic microorganisms, including viruses. However, current understanding of Toll pathway was mainly restricted in mammal and some model insects such as and mosquitoes. Whether plant viruses can also activate the Toll signaling pathway in vector insects is still unknown. In this study, using rice stripe virus (RSV) and its insect vector (small brown planthopper, ) as a model, we found that the Toll pathway was activated upon RSV infection. In comparison of viruliferous and non-viruliferous planthoppers, we found that four Toll pathway core genes (, , , and ) were upregulated in viruliferous planthoppers. When the planthoppers infected with RSV, the expressions of and were rapidly upregulated at the early stage (1 and 3 days post-infection), whereas was upregulated at the late stage (9 days post-infection). Furthermore, induction of Toll pathway was initiated by interaction between a Toll receptor and RSV nucleocapsid protein (NP). Knockdown of increased the proliferation of RSV in vector insect, and the ds-treated insects exhibited higher mortality than that of ds-treated ones. Our results provide the first evidence that the Toll signaling pathway of an insect vector is potentially activated through the direct interaction between Toll receptor and a protein encoded by a plant virus, indicating that Toll immune pathway is an important strategy against plant virus infection in an insect vector.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.613957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821435PMC
January 2021

Natural Melanin/Polyurethane Composites as Highly Efficient Near-Infrared-Photoresponsive Shape Memory Implants.

ACS Biomater Sci Eng 2020 09 21;6(9):5305-5314. Epub 2020 Aug 21.

Institute for Frontier Materials, Australian Future Fibers Research and Innovation Center, Deakin University, 75 Pigdons Road, Geelong, Victoria 3220, Australia.

Natural melanin is recognized as a biocompatible photothermal agent because of its biologically derived nature and efficient photothermal conversion ability. Here, yak hair melanin (YM) is added to polyurethane (PU) for the fabrication of NIR-photoresponsive shape memory implants. The toxicity of the YM/PU composites is carried out by exposing them to human mesenchymal stem cells (hMSCs) and mouse fibroblast (L929) cells lines for 24 h, while the toxicity is investigated by implanting the YM/PU composites in the mouse for two months. No significant differences on cell viability, blood chemistry, hematology, and histological results are observed between YM/PU composites and control groups, suggesting their excellent biocompatibility. The biostability of the YM/PU composites is confirmed by monitoring their degradation for 12 weeks. The YM/PU column implanted in the back subcutis or vagina of the mouse rapidly recovered to its original state within 60 s under a very low NIR laser (808 nm, 0.5 W/cm) intensity, which is much lower than the general laser intensity for photothermal cancer therapy (1-2 W/cm). This work confirms the applicability of the YM/PU composites as long-term implant materials and expedites the use of YM/PU composites as cost-effective candidates for biomedical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsbiomaterials.0c00933DOI Listing
September 2020

Achieving Superior Energy Storage Properties and Ultrafast Discharge Speed in Environment-Friendly Niobate-Based Glass Ceramics.

ACS Appl Mater Interfaces 2021 Jan 14;13(3):4236-4243. Epub 2021 Jan 14.

Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Functional Materials Research Laboratory, School of Materials Science & Engineering, Tongji University, 4800 Caoan Road, Shanghai 201804, China.

Glass ceramics composed of NaO-BaO-BiO-NbO-AlO-SiO (NBBN-AS) were modified by rare-earth doping and prepared via the melt-quenching process accompanied by controlled crystallization. High-resolution transmission electron microscopy displayed the glassy matrix closely encompassing the nanosized NaNbO, BaNaNbO, BaAlSiO, and AlNbO crystalline grains. With rare-earth doping, the NBBN-AS glass ceramics' theoretical energy storage density can reach 22.48 J/cm. This excellent energy storage property is credited with increasing breakdown strength, and numerical simulation was applied to reveal the intrinsic mechanism for increased breakdown strength by rare-earth doping. The charge-discharge results indicated a giant power density of 220 MW/cm as well as an ultrafast discharge speed of 11 ns. The results indicate that the glass ceramic can be used in advanced capacitor applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c21231DOI Listing
January 2021

Complete genome sequence of a novel foveavirus isolated from Allium sativum L. in China.

Arch Virol 2021 Mar 13;166(3):983-986. Epub 2021 Jan 13.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

The complete genome sequence of a novel foveavirus identified in garlic (Allium sativum L.) in China was determined using RNA-seq, reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The entire genomic RNA (GenBank accession MT981417) is 8748 nucleotides long excluding the 3'-terminal poly(A) tail and contains five open reading frames (ORFs). These ORFs encode the viral replicase, a triple gene block, and a coat protein. The virus was tentatively named "garlic yellow stripe associated virus" (GarYSaV). Pairwise comparisons of protein sequences show that GarYSaV encodes proteins that share less than 47% identity with those of other foveaviruses, suggesting that it represents a new species in the genus. Phylogenetic analysis of amino acid sequences of the replicase and CP confirm that GarYSaV is a member of the genus Foveavirus. To our knowledge, this is the first report of a foveavirus in a monocot plant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-04957-8DOI Listing
March 2021

Anthocyanins Are Converted into Anthocyanidins and Phenolic Acids and Effectively Absorbed in the Jejunum and Ileum.

J Agric Food Chem 2021 Jan 11;69(3):992-1002. Epub 2021 Jan 11.

Shaanxi Provincial Bioresource Key Laboratory, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, Shaanxi, China.

Anthocyanins have been known for their health benefits. However, the digestion and absorption of anthocyanins through the gastrointestinal tract have not been fully clarified, creating challenges for understanding why anthocyanins have high biological activities and purported low bioavailability . Twenty-seven male rats were intubated with a 500 mg/kg dose of cyanidin-3-glucoside (C3G). Samples from rats' stomach, duodenum, jejunum, ileum, colon, and serum were collected at 0.5, 1, 2, 3, 4, 5, 6, 12, and 24 h after intubation. Three rats without C3G were used as the control with samples collected at 0 h. C3G and its metabolites in each sample were analyzed using high-performance liquid chromatography-PDA-electrospray ionization-MS/MS. These studies' results unequivocally demonstrated that cyanidin and phenolic acids were the primary C3G metabolites absorbed, mainly in the jejunum and ileum, between 1 and 5 h post-ingestion. We speculate that C3G uses phloroglucinaldehyde and protocatechuic acid metabolic pathways in its metabolism .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c07771DOI Listing
January 2021

LncRNA DHRS4-AS1 Inhibits the Stemness of NSCLC Cells by Sponging miR-224-3p and Upregulating TP53 and TET1.

Front Cell Dev Biol 2020 23;8:585251. Epub 2020 Dec 23.

Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death. This study aimed to examine the roles of DHRS4-AS1/miR-224-3p signaling in the cancer cell stemness of NSCLC. Real-time PCR showed that DHRS4-AS1 was downregulated in cancerous tissues, and bioinformatics analysis revealed that high DHRS4-AS1 expression indicated a good prognosis for NSCLC patients. Sphere and colony formation assays showed that DHRS4-AS1 overexpression significantly suppressed NSCLC cell colony formation and stem cell-like properties. DHRS4-AS1 also abrogated the expression of OCT4, SOX2, CD34, and CD133, markedly inhibited the expression of epithelial-mesenchymal transition (EMT)-related factors, N-cadherin, ZEB1, and Vimentin, and increased E-cadherin expression in spheres. Furthermore, luciferase reporter assays and real-time PCR analysis demonstrated that DHRS4-AS1 and miR-224-3p were antagonistically repressed in NSCLC cells. RNA immunoprecipitation (RIP) analysis revealed that DHRS4-AS1 interacted with miR-224-3p. DHRS4-AS1 partially reversed the miR-224-3p-decreased TP53 and TET1, resulting in the inhibition of tumor growth . Finally, TP53 and TET1 were antagonistically regulated by DHRS4-AS1 and miR-224-3p in NSCLC cells. In conclusion, TP53- and TET1-associated DHRS4-AS1/miR-224-3p axis is an essential mechanism by which NSCLC modulates cancer cell stemness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2020.585251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786137PMC
December 2020

Identification of Two New Isolates of From Different Regions in China: Molecular Diversity, Phylogenetic and Recombination Analysis.

Front Microbiol 2020 23;11:616171. Epub 2020 Dec 23.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.

(ChiVMV) is an important plant pathogen with a wide host range, causing serious yield losses in pepper production all over the world. Recombination is a major evolutionary event for single-stranded RNA viruses, which helps isolates adapt to new environmental conditions and hosts. Recombination events have been identified in multiple potyviruses, but so far, there have been no reports of recombination events among the ChiVMV population. We here detected ChiVMV in pepper samples collected from Guangxi and Yunnan provinces for the first time and amplified the nearly full-length sequences. Phylogenetic and recombination analysis were performed using the new sequences and the 14 full-length and 23 capsid protein (CP) sequences available in GenBank. Isolates tend to cluster on a geographical basis, indicating that geographic-driven evolution may be an important determinant of ChiVMV genetic differences. A total of 10 recombination events were detected among the ChiVMV sequences using RDP4 with a strict algorithm, and both the Guangxi and Yunnan isolates were identified as recombinants. Recombination appears to be a significant factor affecting the diversity of ChiVMV isolates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.616171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785935PMC
December 2020

High-Fidelity, High-Scalability Two-Qubit Gate Scheme for Superconducting Qubits.

Phys Rev Lett 2020 Dec;125(24):240503

Shenzhen Institute for Quantum Science and Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

High-quality two-qubit gate operations are crucial for scalable quantum information processing. Often, the gate fidelity is compromised when the system becomes more integrated. Therefore, a low-error-rate, easy-to-scale two-qubit gate scheme is highly desirable. Here, we experimentally demonstrate a new two-qubit gate scheme that exploits fixed-frequency qubits and a tunable coupler in a superconducting quantum circuit. The scheme requires less control lines, reduces cross talk effect, and simplifies calibration procedures, yet produces a controlled-Z gate in 30 ns with a high fidelity of 99.5%, derived from the interleaved randomized benchmarking method. Error analysis shows that gate errors are mostly coherence limited. Our demonstration paves the way for large-scale implementation of high-fidelity quantum operations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.125.240503DOI Listing
December 2020