Publications by authors named "Fei Xu"

1,504 Publications

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Clinical and genetic study of three families with 15q11q13 duplications.

Taiwan J Obstet Gynecol 2022 Jul;61(4):717-721

Department of Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, China. Electronic address:

Objective: To report three families with chromosome 15q11q13 duplications.

Case Report: We report the prenatal diagnosis and genetic counseling of three 15q11q13 duplications.

Conclusion: Chromosomal microdeletions and microduplications are difficult to be detected by conventional cytogenetics. With molecular genetic techniques including array-based methods, the number of reported cases has rapidly increased. An integration of prenatal ultrasound, NIPT, karyotype analysis, CMA and genetic counseling is helpful for the prenatal diagnosis of chromosomal microdeletions/microduplications.
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http://dx.doi.org/10.1016/j.tjog.2021.12.004DOI Listing
July 2022

Comparative analysis of the structure and function of rhizosphere microbiome of the Chinese medicinal herb Alisma in different regions.

Arch Microbiol 2022 Jul 2;204(7):448. Epub 2022 Jul 2.

Nanjing University of Chinese Medicine School of Pharmacy, 138 Xianlin Avenue, Nanjing, 210023, China.

Rhizoma Alismatis, a commonly used traditional Chinese medicine, is the dried tuber of Alisma orientale and Alisma A. plantago-aquatica, mainly cultivated in Fujian and Sichuan provinces (China), respectively. Studies have shown that the rhizosphere microbiome is a key factor determining quality of Chinese medicinal plants. Here we applied metagenomics to investigate the rhizosphere microbiome of Alisma in Fujian and Sichuan, focusing on its structure and function and those genes involved in protostane triterpenes biosynthesis. The dominant phyla were Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes. Compared with Fujian, the rhizosphere of Sichuan has a greater α diversity and stronger microbial interactions but significantly lower relative abundance of archaea. Microbes with disease-suppressing functions were more abundant in Sichuan than Fujian, but vice versa for those with IAA-producing functions. Gemmatimonas, Anaeromyxobacter, and Pseudolabrys were the main contributors to the potential functional difference in two regions. Genes related to protostane triterpenes biosynthesis were enriched in Fujian. Steroidobacter, Pseudolabrys, Nevskia, and Nitrospira may contribute to the accumulation of protostane triterpenes in Alisma. This work fills a knowledge gap of Alisma's rhizosphere microbiome, providing a valuable reference for studying its beneficial microorganisms.
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http://dx.doi.org/10.1007/s00203-022-03084-5DOI Listing
July 2022

Roles of ursodeoxycholic acid in the bile biochemistry and metabolomics in patients with choledocholithiasis: a prospective study.

Metabolomics 2022 Jul 1;18(7):46. Epub 2022 Jul 1.

Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, No. 150 Jimo Road, Pudong New District, Shanghai, 200120, China.

Introduction: Recurrence after the endoscopic treatment of common bile duct stones (CBDS) is related to bile metabolism and bile compositions. Ursodeoxycholic acid (UDCA) has been proved effective in reducing the recurrence of CBDS. However, the detailed effects of UDCA on bile metabolism are still not extensively explored.

Objectives: This study aimed to analyze the role of UDCA in patients with choledocholithiasis (CDC) from the perspective of biochemistry and metabolomics.

Methods: A total of 89 patients with CDC who underwent endoscopic retrograde cholangiopancreatography were prospectively examined and randomly assigned to control and UDCA groups. The biochemical detections (cholesterol, bilirubin, and so on) were performed on the collected bile. Moreover, the metabolomics analysis was conducted based on bile from 20 patients in the UDCA group.

Results: The bile levels of cholesterol and endotoxins significantly decreased after UDCA treatment. Regarding bile metabolomics, the levels of 25 metabolites changed significantly after UDCA treatment. The pathway enrichment analysis showed that the UDCA addition evoked a common response related to phenylalanine, tyrosine, and tryptophan biosynthesis; phenylalanine metabolism; arachidonic acid metabolism; and terpenoid backbone biosynthesis.

Conclusions: UDCA treatment within a short time interval (7 days) did not improve the circulating laboratory values in patients with CDC who had undergone endoscopy surgery. However, relevant decreases in the bile levels of cholesterol and endotoxin were observed. UDCA evoked a common response related to lipid metabolism and amino acid metabolism, which probably reduced the bile level of cholesterol, protected hepatocytes, and corrected the abnormality of lipid metabolism caused by CDC.
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http://dx.doi.org/10.1007/s11306-022-01906-7DOI Listing
July 2022

Exploring the influence of intermolecular hydrogen bonding on the fluorescence properties of HQCT and HQPH fluorescent chemosensors.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 20;280:121537. Epub 2022 Jun 20.

School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, PR China.

Recently, two trace water detection probes, 8-hydroxyquinoline-2-carboxaldehyde thiosemicarbazone(HQCT) and 8-hydroxyquinoline-2-carboxaldehyde (pyridine-2-carbonyl)-hydrazine(HQPH) have been successfully designed in the experiment. The original intramolecular proton transfer can be prevented by the water molecules, leading to fluorescence quenching. In order to investigate the fluorescence quenching mechanism, the effect of water molecules on the excited state proton transfer process will be studied in detail. In this contribution, the six models have been optimized and the related analysis have been carried out. When water molecules are involved in the proton transfer process, the energy barrier decreases significantly and the conversion of the enol structure to the keto structure is accelerated. Moreover, the intermolecular hydrogen bonding, not participating in the proton transfer process, can facilitate the proton transfer process by affecting the distribution of the electrostatic potential within the molecule, which in turn lowers the energy barrier for proton transfer.
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http://dx.doi.org/10.1016/j.saa.2022.121537DOI Listing
June 2022

A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells.

J Mater Chem B 2022 Jun 29. Epub 2022 Jun 29.

Department of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China.

A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization. experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.
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http://dx.doi.org/10.1039/d2tb00721eDOI Listing
June 2022

Responsive change of crop-specific soil bacterial community to cadmium in farmlands surrounding mine area of Southeast China.

Environ Res 2022 Jun 21:113748. Epub 2022 Jun 21.

Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, PR China. Electronic address:

In arable soils co-influenced by mining and farming, soil bacteria significantly affect metal (Cadmium, Cd) bioavailability and accumulation. To reveal the soil microecology response under this co-influence, three intersection areas (cornfield, vegetable field, and paddy field) were investigated. With a similar nutrient condition, the soils showed varied Cd levels (0.31-7.70 mg/kg), which was negatively related to the distance from mining water flow. Different soils showed varied microbial community structures, which was dominated by Chloroflexi (19.64-24.82%), Actinobacteria (15.49-31.96%), Acidobacteriota (9.46-20.31%), and Proteobacteria (11.88-14.57%) phyla. A strong correlation was observed between functional microbial taxon (e. g. Acidobacteriota), soil physicochemical properties, and Cd contents. The relative abundance of tolerant bacteria including Vicinamibacteraceae, Knoellia, Ardenticatenales, Lysobacter, etc. elevated with the increase of Cd, which contributed to the enrichment of heavy metal resistance genes (HRGs) and integration genes (intlI), thus enhancing the resistance to heavy metal pollution. Cd content rather than crop species was identified as the dominant factor that influenced the bacterial community. Nevertheless, the peculiar agrotype of the paddy field contributed to its higher HRGs and intlI abundance. These results provided fundamental information about the crop-specific physiochemical-bacterial interaction, which was helpful to evaluate agricultural environmental risk around the intersection of farmland and pollution sources.
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http://dx.doi.org/10.1016/j.envres.2022.113748DOI Listing
June 2022

Thiourea derivatives acting as functional monomers of As(Ш) molecular imprinted polymers: A theoretical and experimental study on binding mechanisms.

J Hazard Mater 2022 05 17;430:128508. Epub 2022 Feb 17.

School of Medical Instrument and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address:

Thiourea derivatives are expected to be potential monomers of As(Ш) molecular imprinted polymers (MIPs) which are used to specifically recognize As(Ш). However, the specific recognition and binding mechanisms between template and monomers are unclear, which limits the practical applications of MIPs in As(Ш)detection. In this work, density functional theory (DFT) calculations, molecular dynamics (MD) simulations and experimental methods were jointly applied to explore the binding interactions between HAsO and thiourea derivatives and environmental factors influences, aiming to find out the best monomer and optimal preparation conditions for HAsO MIPs. Among five monomer candidates, (2, 6-difluorophenyl) thiourea (FT) was calculated to be the most potential one, while allyl thiourea (AT) was the second choice. Configurations of the most stable binding complexes were found out. The optimal solvent was found to be toluene and the bindings were more favorable at pH 7.5 in aqueous solution. Besides, EGDMA was proved as the best cross-linker with the optimal ratio of template: monomer: cross-linker= 2:3:20. Moreover, the binding interactions were identified to be hydrogen bonds, and the non-covalent nature was revealed. These findings provide references for efficient design and preparation of good-performance HAsO MIPs, which can be used to detect and remove As(Ш) from environment.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128508DOI Listing
May 2022

A Web-Based mHealth Intervention With Telephone Support to Increase Physical Activity Among Pregnant Patients With Overweight or Obesity: Feasibility Randomized Controlled Trial.

JMIR Form Res 2022 Jun 22;6(6):e33929. Epub 2022 Jun 22.

Division of Research, Kaiser Permanente Northern California, Oakland, CA, United States.

Background: Pregnant patients with overweight or obesity are at high risk for perinatal complications. Excess gestational weight gain (GWG) further exacerbates this risk. Mobile health (mHealth) lifestyle interventions that leverage technology to facilitate self-monitoring and provide just-in-time feedback may motivate behavior change to reduce excess GWG, reduce intervention costs, and increase scalability by improving access.

Objective: This study aimed to test the acceptability and feasibility of a pilot mHealth lifestyle intervention for pregnant patients with overweight or obesity to promote moderate intensity physical activity (PA), encourage guideline-concordant GWG, and inform the design of a larger pragmatic cluster randomized controlled trial.

Methods: We conducted a mixed methods acceptability and feasibility randomized controlled trial among pregnant patients with a prepregnancy BMI of 25 to 40 kg/m. Patients with singletons at 8 to 15 weeks of gestation who were aged ≥21 years and had Wi-Fi access were recruited via email from 2 clinics within Kaiser Permanente Northern California and randomized to receive usual prenatal care or an mHealth lifestyle intervention. Participants in the intervention arm received wireless scales, access to an intervention website, activity trackers to receive automated feedback on weight gain and activity goals, and monthly calls from a lifestyle coach. Surveys and focus groups with intervention participants assessed intervention satisfaction and ways to improve the intervention. PA outcomes were self-assessed using the Pregnancy Physical Activity Questionnaire, and GWG was assessed using electronic health record data for both arms.

Results: Overall, 33 patients were randomly assigned to the intervention arm, and 35 patients were randomly assigned to the usual care arm. All participants in the intervention arm weighed themselves at least once a week, compared with 20% (7/35) of the participants in the usual care arm. Participants in the intervention arm wore the activity tracker 6.4 days per week and weighed themselves 5.3 times per week, and 88% (29/33) of them rated the program "good to excellent." Focus groups found that participants desired more nutrition-related support to help them manage GWG and would have preferred an app instead of a website. Participants in the intervention arm had a 23.46 metabolic equivalent of task hours greater change in total PA per week and a 247.2-minute greater change in moderate intensity PA per week in unadjusted models, but these effects were attenuated in adjusted models (change in total PA: 15.55 metabolic equivalent of task hours per week; change in moderate intensity PA: 199.6 minutes per week). We found no difference in total GWG (mean difference 1.14 kg) compared with usual care.

Conclusions: The pilot mHealth lifestyle intervention was feasible, highly acceptable, and promoted self-monitoring. Refined interventions are needed to effectively affect PA and GWG among pregnant patients with overweight or obesity.

Trial Registration: ClinicalTrials.gov NCT03936283; https://clinicaltrials.gov/ct2/show/NCT03936283.
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http://dx.doi.org/10.2196/33929DOI Listing
June 2022

Clinical, radiologic, and physiologic features of idiopathic pulmonary fibrosis (IPF) with and without emphysema.

Expert Rev Respir Med 2022 Jun 29:1-9. Epub 2022 Jun 29.

Department of Pulmonary and Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, P.R.China.

Background: Idiopathic pulmonary fibrosis (IPF) can combine with emphysema, a condition termed as IPF with emphysema (IPFE). We compared the clinical, radiologic, and physiologic features of IPF and IPFE.

Research Design And Methods: Newly diagnosed IPF    and IPFE    patients were recruited between January 2018 and September 2020. Symptoms, high resolution computed tomography (HRCT), pulmonary function test (PFT) data, composite physiologic index (CPI), gender-age-physiology (GAP) scores, and follow-up data were obtained.

Results: The IPFE group had greater proportion of male smokers, and of lung cancer cases. The IPFE group had higher VC, FVC FEV, and lower FEV/FVC and DL and lower percent fibrosis on HRCT. Both groups had similar symptoms and mortality. Mortality rate was associated with inability to perform PFT, CPI, GAP scores, percent fibrosis, VC, FVC, FEV and DL, serum SCC-Ag and CA125, and anti-fibrotic therapy (≥12 months) in IPF, while it was associated with inability to perform PFT, CPI, percent fibrosis, DL, serum CEA, CYFRA21-1 and CA125, and anti-fibrotic therapy (≥12 months) in IPFE.

Conclusion: IPF and IPFE patients are different in smoking history, physiologic indices, HRCT patterns and prognostic factors, however, they have similar mortality. Anti-fibrotic therapy could improve the survival rate in both IPF and IPFE.
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http://dx.doi.org/10.1080/17476348.2022.2093717DOI Listing
June 2022

[Investigation and health risk assessment of veterinary drug residues in chickens and eggs sold in Ningxia from 2016 to 2020].

Wei Sheng Yan Jiu 2022 May;51(3):497-508

Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China.

Objective: To understand the monitoring status of veterinary drug residues in chickens and eggs sold in Ningxia from 2016 to 2020, and to evaluate the health risks of human intake.

Methods: A total of 303 chicken and 237 eggs were collected from 2016 to 2020, and the veterinary drug residues in chickens and eggs sold in Ningxia were detected and analyzed by high performance liquid chromatography-tandem mass spectrometry. The food safety index method was used to assess the health risks in combination with the dietary intake of residents.

Results: The detection rate of veterinary drug residues in chicken was 38.0%(115/303), and the over-standard rate was 7.6%(23/303). The detection rate of veterinary drug residues in eggs was 26.6%(63/237), and the over-standard rate was 19.8%(47/237). The food safety index of doxycycline in chicken was 4.59, and the food safety indexes of doxycycline, flumequine and flufenicol in eggs were 7.09, 26.5 and 2.33, respectively, all of which were much higher than 1, suggesting that there were health risks. The food safety indexes of other substances were all less than 1, and the food safety indexes were all acceptable.

Conclusion: Veterinary drugs were widely detected in chicken and eggs in Ningxia from 2016 to 2020. Some veterinary drugs had hidden health risks.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.025DOI Listing
May 2022

Correction to: Correlation between lordosis distribution index, lordosis tilt, and occurrence of proximal junctional kyphosis following surgery for adult degenerative scoliosis.

Eur Spine J 2022 Jun 18. Epub 2022 Jun 18.

Orthopaedic Department, Peking University Third Hospital, Haidian District, No. 49 North Garden Road, Beijing, 100191, China.

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http://dx.doi.org/10.1007/s00586-022-07274-zDOI Listing
June 2022

Thermal-Switchable, Trifunctional Ceramic-Hydrogel Nanocomposites Enable Full-Lifecycle Security in Practical Battery Systems.

ACS Nano 2022 Jun 16. Epub 2022 Jun 16.

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Thermal runaway (TR) failures of large-format lithium-ion battery systems related to fires and explosions have become a growing concern. Here, we design a smart ceramic-hydrogel nanocomposite that provides integrated thermal management, cooling, and fire insulation functionalities and enables full-lifecycle security. The glass-ceramic nanobelt sponges exhibit high mechanical flexibility with 80% reversible compressibility and high fatigue resistance, which can firmly couple with the polymer-nanoparticle hydrogels and form thermal-switchable nanocomposites. In the operating mode, the high enthalpy of the nanocomposites enables efficient thermal management, thereby preventing local temperature spikes and overheating under extremely fast charging conditions. In the case of mechanical or thermal abuse, the stored water can be immediately released, leaving behind a highly flexible ceramic matrix with low thermal conductivity (42 mW m K at 200 °C) and high-temperature resistance (up to 1300 °C), thus effectively cooling the TR battery and alleviating the devastating TR propagation. The versatility, self-adaptivity, environmental friendliness, and manufacturing scalability make this material highly attractive for practical safety assurance applications.
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http://dx.doi.org/10.1021/acsnano.2c02557DOI Listing
June 2022

The Homogeneous Gas-Phase Formation Mechanism of PCNs from Cross-Condensation of Phenoxy Radical with 2-CPR and 3-CPR: A Theoretical Mechanistic and Kinetic Study.

Int J Mol Sci 2022 May 24;23(11). Epub 2022 May 24.

Environment Research Institute, Shandong University, Qingdao 266237, China.

Chlorophenols (CPs) and phenol are abundant in thermal and combustion procedures, such as stack gas production, industrial incinerators, metal reclamation, etc., which are key precursors for the formation of polychlorinated naphthalenes (PCNs). CPs and phenol can react with H or OH radicals to form chlorophenoxy radicals (CPRs) and phenoxy radical (PhR). The self-condensation of CPRs or cross-condensation of PhR with CPRs is the initial and most important step for PCN formation. In this work, detailed thermodynamic and kinetic calculations were carried out to investigate the PCN formation mechanisms from PhR with 2-CPR/3-CPR. Several energetically advantageous formation pathways were obtained. The rate constants of key elementary steps were calculated over 600~1200 K using the canonical variational transition-state theory (CVT) with the small curvature tunneling (SCT) contribution method. The mechanisms were compared with the experimental observations and our previous works on the PCN formation from the self-condensation of 2-CPRs/3-CPRs. This study shows that naphthalene and 1-monochlorinated naphthalene (1-MCN) are the main PCN products from the cross-condensation of PhR with 2-CPR, and naphthalene and 2-monochlorinated naphthalene (2-MCN) are the main PCN products from the cross-condensation of PhR with 3-CPR. Pathways terminated with Cl elimination are preferred over those terminated with H elimination. PCN formation from the cross-condensation of PhR with 3-CPR can occur much easier than that from the cross-condensation of PhR with 2-CPR. This study, along with the study of PCN formation from the self-condensation 2-CPRs/3-CPRs, can provide reasonable explanations for the experimental observations that the formation potential of naphthalene is larger than that of 1-MCN using 2-CP as a precursor, and an almost equal yield of 1-MCN and 2-MCN can be produced with 3-CP as a precursor.
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http://dx.doi.org/10.3390/ijms23115866DOI Listing
May 2022

Evolutionary and Regulatory Pattern Analysis of Soybean Ca ATPases for Abiotic Stress Tolerance.

Front Plant Sci 2022 19;13:898256. Epub 2022 May 19.

Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

P-type Ca ATPases are responsible for cellular Ca transport, which plays an important role in plant development and tolerance to biotic and abiotic stresses. However, the role of P-type Ca ATPases in stress response and stomatal regulation is still elusive in soybean. In this study, a total of 12 P-type Ca ATPases genes ( and ) were identified from the genome of . We analyzed the evolutionary relationship, conserved motif, functional domain, gene structure and location, and promoter elements of the family. Chlorophyll fluorescence imaging analysis showed that vegetable soybean leaves are damaged to different extents under salt, drought, cold, and shade stresses. Real-time quantitative PCR (RT-qPCR) analysis demonstrated that most of the and are up-regulated after drought, cold, and NaCl treatment, but are down-regulated after shading stress. Microscopic observation showed that different stresses caused significant stomatal closure. Spatial location and temporal expression analysis suggested that , and might promote stomatal closure under drought, cold, and salt stress. might regulate stomatal closure in shading stress. and might have a negative function on cold stress. The results laid an important foundation for further study on the function of P-type Ca ATPase genes and for breeding abiotic stress-tolerant vegetable soybean.
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http://dx.doi.org/10.3389/fpls.2022.898256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161174PMC
May 2022

Combined lifestyle factors on mortality among the elder population: evidence from a Chinese cohort study.

BMC Geriatr 2022 06 1;22(1):474. Epub 2022 Jun 1.

School of Nursing and Health, Zhengzhou University, Zhengzhou, China.

Background: Numerous studies have suggested that lifestyle-related factors are associated with mortality, however limited evidence is available for the Chinese elder population.

Methods: The data of this study was obtained from the Chinese Longitudinal Health Survey (CLHLS) during 2008 - 2018, lifestyle-related factors including body mass index (BMI), smoking, drinking, consumption of vegetables and fruits, physical activity and sleep duration were included as dependent variables in the analysis. A lifestyle risk score was created using six unhealthy behaviors: smoking, drinking, unhealthy weight, physical inactivity, not eat vegetables or fruits and short or prolonged sleep. The Kaplan-Meier curves were used to illustrate the cumulative effect of lifestyle factors on mortality and cox regression models were conducted to estimate the combined effects of lifestyle-related factors on total mortality.

Results: The results illustrated that low BMI, smoking, no fruit eating, and no physical inactivity were risk factors for total mortality. KM curves showed significant cumulative effect of unhealthy lifestyle factors on mortality. Compared with participants without any unhealthy factors, the hazard ratio (HR) for participants with six unhealthy factors was 1.335 (1.015,1.757) for all-cause mortality.

Conclusions: This study demonstrated poor adherence to a healthy lifestyle may increase all-cause mortality and specific combinations of lifestyle related factors have different effects on mortality among Chinese elderly population.
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http://dx.doi.org/10.1186/s12877-022-03017-3DOI Listing
June 2022

Upregulation of Ferroptosis-Related Fanconi Anemia Group D2 is a Poor Prognostic Factor and an Indicator of Tumor Immune Cell Infiltration in Lung Adenocarcinoma.

Front Genet 2022 11;13:825685. Epub 2022 May 11.

Department of Geriatric Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.

Fanconi anemia (FA) group D2 () is a ferroptosis-related gene crucial for DNA damage repair and negative ferroptosis regulation. Our study aimed to evaluate its prognostic value as well as its association with ferroptosis and immune infiltration in lung adenocarcinoma (LUAD). Transcriptome sequencing data, clinical information, and immunohistochemistry data were collected from the TCGA, GEO, and HPA databases, respectively, for three independent cohorts. Univariate and multivariate analyses were used to assess the correlations between FANCD2 expression and overall survival or clinicopathological parameters. cBioPortal was utilized to investigate the alteration status. Gene and protein networks based on FANCD2 interactions were generated using GeneMANIA and STRING, respectively. Based on the CancerSEA database, the function of FANCD2 was explored at the single-cell level. The relationships between expression levels and tumor-infiltrating immune cells and their equivalent gene signatures were analyzed using TIMER, GEPIA, TISIDB, and ssGSEA databases. CIBERSORT was used to analyze the relevance of the infiltration of 24 types of immune cells. The results revealed that FANCD2 expression was significantly upregulated in LUAD and lung squamous cell carcinoma (LUSC) tissues than that in normal tissues. Further, the overexpression of FANCD2 was closely associated with poor survival for Patients with LUAD but not for patients with LUSC. FANCD2 expression levels were related to tumor-infiltrating immune cells and their matching gene signatures, including CD8 T cells, natural killer (NK) cells, dendritic cells (DC), and Th2 cells in cases of LUAD. Therefore, FANCD2 was identified as a crucial molecule underlying the synergistic effects of ferroptosis and immunotherapy for Patients with LUAD.
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http://dx.doi.org/10.3389/fgene.2022.825685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130730PMC
May 2022

ZIF-90 with biomimetic Zn-N coordination structures as an effective nanozyme to mimic natural hydrolase.

Nanoscale 2022 Jun 9;14(22):7985-7990. Epub 2022 Jun 9.

Department of Physics, Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Xiamen University, Xiamen 361005, P. R. China.

Inspired by the structures of enzymes, a fast and robust strategy for generating ZIF-90 metallo-nanozymes is presented. The Zn-N coordination structure in ZIF-90 can closely imitate the catalytic center of a natural zinc-based hydrolase. As expected, ZIF-90 possesses potent hydrolase-mimicking activity, high stability and excellent recyclability.
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http://dx.doi.org/10.1039/d2nr01213hDOI Listing
June 2022

First-line immunotherapy or angiogenesis inhibitor combined with chemotherapy for advanced non-small cell lung cancer with EGFR exon 20 insertions: Real-world evidence from China.

Cancer Med 2022 May 24. Epub 2022 May 24.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Chaoyang, Beijing, China.

Background: Currently, survival benefit of immunotherapy in advanced non-small cell lung cancer (NSCLC) with EGFR exon 20 insertions (ex20ins) is controversial, though it generally indicates poor response and activity. Compared with standard chemotherapy in combination with bevacizumab, first-line chemotherapy plus immune checkpoint inhibitor (ICI) in advanced NSCLC with EGFR ex20ins remains elusive and lacks real-world evidence.

Patients And Methods: A retrospective real-world study was conducted to evaluate clinical outcomes of chemotherapy alone (C), chemotherapy plus ICI (C + I), or chemotherapy plus angiogenesis inhibitors (C + A) as first-line strategies for advanced NSCLC patients with EGFR ex20ins. Investigator-assessed response and survival outcomes were compared between subgroups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was conducted to reveal concomitant alterations and explore the molecular landscape of ex20ins.

Results: A total of 164 patients were screened, identifying 35 kinds of ex20ins, and 122 cases treated with C, C + I, and C + A were finally included in the first-line analysis. C + A achieved much better objective response rate (ORR, 38.1% vs. 18.2%) and significant progression-free survival (PFS) benefit compared with C (median, 7.73 vs.5.93 months, HR = 0.60, 95% CI: 0.40-0.90, p = 0.014), and it showed similar ORR (38.1% vs. 40.0%), but higher disease control rate (DCR, 96.8% vs. 80.0%) and numerically longer median PFS (7.73 vs. 6.53 months, HR = 0.83, 95% CI: 0.44-1.56, p = 0.30) than C + I. There was no PFS difference between C + I and C, despite of PD-L1 expression or tumor mutational burden. KEGG analysis revealed concomitant upregulation of PI3K/AKT signaling might mediate intrinsic resistance to ICI in ex20ins.

Conclusion: First-line chemotherapy plus angiogenesis inhibitors might yield more survival benefits than chemotherapy alone for NSCLC with EGFR ex20ins, whereas, it suggests that chemotherapy in combination with ICI might not obtain a better survival benefit for this subset of patients. Activation of PI3K/AKT signaling might mediate intrinsic immunosuppression in NSCLC with EGFR ex20ins.
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http://dx.doi.org/10.1002/cam4.4852DOI Listing
May 2022

A fast, sensitive, low-cost electrochemical paper-based chip for real-time simultaneous detection of cadmium (Ⅱ) and lead (Ⅱ) via aptamer.

Talanta 2022 Sep 14;247:123548. Epub 2022 May 14.

Shanghai Engineering Research Centre for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai, China. Electronic address:

A novel electrochemical paper-based microfluidic chip was firstly developed to simultaneously detect cadmium (Ⅱ) and lead (Ⅱ) in vegetable and fruit samples. The patterned filter paper was prepared through the printing of three-electrode patterns on filter paper using an automatic screen-printing machine. Portable and low-cost (less than $1) electrochemical paper-based chips are prepared by filling conductive ink and hot pressing. The paper-based chip could realize signal amplification through gold nanoparticles and seed solutions. Cadmium (Ⅱ) and lead (Ⅱ) were sensitively detected by their aptamers labeled with methylene blue and ferrocene, separately. Under the optimal experimental parameters, the paper-based chip detected cadmium (Ⅱ) and lead (Ⅱ) as low as 23.31 and 46.23 pmol/L (3σ) with a wide linear range from 0.1 to 1000 nmol/L and exhibited excellent selectivity. The RSD was 6.41% (cadmium (Ⅱ)) and 4.20% (lead (Ⅱ)). Compared with other methods, the paper-based chip could complete the detection within 15 min and could be stored at -20 °C for 5 days. Furthermore, the results for vegetable and fruit samples were agreed with the results of the graphite furnace atomic absorption spectrophotometer, in which the recovery rates were 93.20%-95.80%.
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http://dx.doi.org/10.1016/j.talanta.2022.123548DOI Listing
September 2022

A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation.

Bioact Mater 2023 Jan 11;19:666-677. Epub 2022 May 11.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China.

An increased level of reactive oxygen species (ROS) plays a major role in endothelial dysfunction and vascular smooth muscle cell (VSMC) proliferation during in-stent thrombosis and restenosis after coronary artery stenting. Herein, we report an electrospun core-shell nanofiber coloaded with 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL) and rapamycin (RAPA) that correspondingly serves as an ROS scavenger and VSMC inhibitor. This system has the potential to improve the biocompatibility of current drug-eluting stent (DES) coatings with the long-term and continuous release of TEMPOL and rapamycin. Moreover, the RAPA/TEMPOL-loaded membrane selectively inhibited the proliferation of VSMCs while sparing endothelial cells (ECs). This membrane demonstrated superior ROS-scavenging, anti-inflammatory and antithrombogenic effects in ECs. In addition, the membrane could maintain the contractile phenotype and mitigate platelet-derived growth factor BB (PDGF-BB)-induced proliferation of VSMCs. results further revealed that the RAPA/TEMPOL-loaded covered stents promoted rapid restoration of vascular endothelium compared with DES and persistently impeded inflammation and neointimal hyperplasia in porcine models.
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http://dx.doi.org/10.1016/j.bioactmat.2022.04.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114161PMC
January 2023

Hydrogels for Tissue Engineering: Addressing Key Design Needs Toward Clinical Translation.

Front Bioeng Biotechnol 2022 5;10:849831. Epub 2022 May 5.

Department of Chemical Engineering, McMaster University, Hamilton, ON, Canada.

While the soft mechanics and tunable cell interactions facilitated by hydrogels have attracted significant interest in the development of functional hydrogel-based tissue engineering scaffolds, translating the many positive results observed in the lab into the clinic remains a slow process. In this review, we address the key design criteria in terms of the materials, crosslinkers, and fabrication techniques useful for fabricating translationally-relevant tissue engineering hydrogels, with particular attention to three emerging fabrication techniques that enable simultaneous scaffold fabrication and cell loading: 3D printing, tissue engineering, and cell electrospinning. In particular, we emphasize strategies for manufacturing tissue engineering hydrogels in which both macroporous scaffold fabrication and cell loading can be conducted in a single manufacturing step - electrospinning, 3D printing, and tissue engineering. We suggest that combining such integrated fabrication approaches with the lessons learned from previously successful translational experiences with other hydrogels represents a promising strategy to accelerate the implementation of hydrogels for tissue engineering in the clinic.
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http://dx.doi.org/10.3389/fbioe.2022.849831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119391PMC
May 2022

Hybrid Photochromic Lanthanide Phosphonate with Multiple Photoresponsive Functionalities.

Inorg Chem 2022 May 20;61(21):8379-8385. Epub 2022 May 20.

College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong 266071, P. R. China.

Hybrid photochromic materials (HPMs) with specific photoresponsive functionality have applications in many fields. The photoinduced electron-transfer (ET) strategy has been proved to be effective in the synthesis of HPMs with diverse photomodulated properties. The exploitation of new electron acceptors (EAs) is meaningful for promoting the development of HPMs. In this work, we introduced a rigid tetraimidazole derivative, 3,3,5,5-tetra(imidazol-1-yl)-1,1-biphenyl (TIBP) as a potential EA, into a metal-diphosphonate (1-hydroxyethylidene-1,1-diphosphonic acid, H-HEDP) system to explore HPMs and finally obtained a hybrid metal phosphonate (H-TIBP)·[Dy(H-HEDP) (H-HEDP)]·HO (). features anionic chains composed of diphosphonate and Dy ions. The extra charge is balanced by protonated TIBP cations, which exist in the void of adjacent chains and form H-bonds with O (N-H···O). Upon photostimulation with a Xe lamp (300 W), the crystalline sample exhibited coloration by changing from colorless to pale yellow because of the presence of photoinduced radicals that originated from the ET from O to N. Along with the coloration, photomodulated fluorescence, magnetism, and proton conductivity were also detected in the photoactivated samples. Different from the reported HPMs based on polypyridine derivatives and photoactive species such as pyridinium and naphthalimide derivatives as EAs, our study provides a new category of EA units to yield HPMs with fascinating photoresponsive functionality via the assembly of polyimidazole derivatives and phosphonate-based supramolecular building blocks.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01217DOI Listing
May 2022

Adebrelimab or placebo plus carboplatin and etoposide as first-line treatment for extensive-stage small-cell lung cancer (CAPSTONE-1): a multicentre, randomised, double-blind, placebo-controlled, phase 3 trial.

Lancet Oncol 2022 06 13;23(6):739-747. Epub 2022 May 13.

Department of Thoracic Oncology, Jilin Cancer Hospital, Changchun, China. Electronic address:

Background: Extensive-stage small-cell lung cancer (ES-SCLC) is associated with poor prognosis and treatment options are scarce. Immunotherapy has shown robust clinical activity in ES-SCLC in previous phase 3 trials. We aimed to assess the efficacy and safety of adebrelimab (SHR-1316), a novel anti-PD-L1 antibody, with standard chemotherapy as a first-line treatment for ES-SCLC.

Methods: The CAPSTONE-1 study was a randomised, double-blind, placebo-controlled, phase 3 trial, done in 47 tertiary hospitals in China. Key inclusion criteria were patients aged 18-75 years, with previously untreated histologically or cytologically confirmed ES-SCLC and an Eastern Cooperative Oncology Group (ECOG) performance status of 0-1. Eligible patients were randomly assigned (1:1) to receive four to six cycles of carboplatin (area under the curve of 5 mg/mL per min, day 1 of each cycle) and etoposide (100 mg/m of body-surface area, on days 1-3 of each cycle) with either adebrelimab (20 mg/kg, day 1 of each cycle) or matching placebo, followed by maintenance therapy with adebrelimab or placebo. All treatments were given intravenously in 21-day cycles. Randomisation was done using a centralised interactive web response system with a block size of four, stratified by liver metastases, brain metastases, and lactate dehydrogenase concentration. The primary endpoint was overall survival in patients who received at least one dose of study medication. Safety was analysed in the as-treated population. This study is complete and registered with ClinicalTrials.gov, NCT03711305.

Findings: Between Dec 26, 2018, and Sept 4, 2020, 462 eligible patients were enrolled and randomly assigned: 230 (50%) patients received adebrelimab plus chemotherapy (adebrelimab group) and 232 (50%) patients received placebo plus chemotherapy (placebo group). At data cutoff (Oct 8, 2021), median follow-up was 13·5 months (IQR 8·9-20·1). Median overall survival was significantly improved in the adebrelimab group (median 15·3 months [95% CI 13·2-17·5]) compared with the placebo group (12·8 months [11·3-13·7]; hazard ratio 0·72 [95% CI 0·58-0·90]; one-sided p=0·0017). The most common treatment-related grade 3 or 4 adverse events were decreased neutrophil count (174 [76%] patients in the adebrelimab group and 175 [75%] patients in the placebo group), decreased white blood cell count (106 [46%] and 88 [38%]), decreased platelet count (88 [38%] and 78 [34%]), and anaemia (64 [28%] and 66 [28%]). Treatment-related serious adverse events occurred in 89 (39%) patients in the adebrelimab group and 66 (28%) patients in the placebo group. Four treatment-related deaths were reported: two each in the adebrelimab group (respiratory failure and interstitial lung disease and pneumonia) and placebo group (multiple organ dysfunction and unknown cause of death).

Interpretation: Adding adebrelimab to chemotherapy significantly improved overall survival with an acceptable safety profile in patients with ES-SCLC, supporting this combination as a new first-line treatment option for this population.

Funding: Jiangsu Hengrui Pharmaceuticals.
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http://dx.doi.org/10.1016/S1470-2045(22)00224-8DOI Listing
June 2022

Alteromonas oceanisediminis sp. nov., isolated from deep-sea sediment.

Arch Microbiol 2022 May 16;204(6):325. Epub 2022 May 16.

State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China.

A Gram-stain-negative, aerobic and rod-shaped bacterium, designated strain SM 2104, was isolated from a deep-sea sediment sample collected from the Southwest Indian Ocean. Strain SM 2104 grew at 10-37 °C (optimum at 25 °C), and with 1.0-9.0% (w/v, optimum with 2-4%) NaCl. It hydrolyzed starch, tween 80 and gelatin but did not reduced nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM 2104 was affiliated with the genus Alteromonas, sharing the highest 16S rRNA gene sequence similarities with type strains of Alteromonas flava (97.5%) and Alteromonas facilis (97.4%) and forming a distinct clade together with the two Alteromonas species. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM 2104 and type strains of Alteromonas flava and Alteromonas facilis were below 14.5%, and 71.0%, respectively. The major fatty acids of strain SM 2104 were summed feature 3 (Cω6c/Cω7c), C and summed feature 8 (Cω7c/Cω6c). The major polar lipids of strain SM 2104 were phosphatidylethanolamine and phosphatidylglycerol and the only respiratory quinone of strain SM 2104 was ubiquinone-8. The genomic DNA G + C content of strain SM 2104 was 48.0%. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic analyses presented in this study, strain SM 2104 is considered to represent a novel species within the genus Alteromonas, for which the name Alteromonas oceansediminis sp. nov. is proposed. The type strain is SM 2104 (= CCTCC AB 2021121 = KCTC 82867).
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http://dx.doi.org/10.1007/s00203-022-02908-8DOI Listing
May 2022

Assessing the environmental risk and mobility of cobalt in sediment near nonferrous metal mines with risk assessment indexes and the diffusive gradients in thin films (DGT) technique.

Environ Res 2022 Sep 12;212(Pt C):113456. Epub 2022 May 12.

College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan, 637009, China; Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agriculture Sciences, Beijing, 100081, China. Electronic address:

The Jialing River is the tributary of the Yangtze River with the largest drainage area. In recent years, the Jialing River has suffered a series of environmental problems, such as discharge of industrial effluent and sand mining activities, which have severely threatened the aquatic ecosystem of the river. In the present study, we employed risk assessment indexes, sequential extraction and the diffusive gradients in thin films (DGT) technique to assess environmental risks and study the remobilization of cobalt (Co) in sediments. The potential ecological risk index and risk assessment code results demonstrated that Co may pose a low environmental and ecological risk to the local aquatic environment. However, BCR sequential extraction showed that the sum of the F1, F2 and F3 fractions of Co still accounted for over 50% of the Co in the study areas, indicating that sediments may be a source of Co release. The DGT results showed an increasing trend for DGT-labile Co in deep sediments (-8 cm to -12 cm), and the calculated flux values ranged from 0.08 to 15.54 ng cm·day, indicating that Co tends to transfer across the sediment-water interface at all sampling sites. Correlation analysis showed that F1-Co, F2-Co and F3-Co are the fractions readily captured by DGT and can be used for predicting Co remobilization in sediment. Sand mining activities contribute substantially to the release of Co from the F1 and F3 fractions as a result of strong stirring of sediments and introduction of oxygen into the sediments. The reductive dissolution of iron (Fe) and manganese (Mn) hydroxides or oxides causes the release of Co and Fe/Mn in the sediment, which leads to Co release from the reducible fraction. The above work suggests that sand mining in the Jialing River should be reasonably regulated to prohibit illegal sand mining activities.
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http://dx.doi.org/10.1016/j.envres.2022.113456DOI Listing
September 2022

Establishment and Application of the Assessment System on Ecosystem Health for Restored Urban Rivers in North China.

Int J Environ Res Public Health 2022 05 5;19(9). Epub 2022 May 5.

Beijing Municipal Research Institute of Eco-Environment Protection, National Engineering Research Center for Urban Environmental Pollution Control, Beijing 100037, China.

The study on ecosystem health evaluation for restored urban rivers is of specific significance to improving river health and realizing the adaptive management for urban river ecosystems. Based on the health definition of restored urban rivers in North China, this study attempted to set up a system of alternative indicators on ecosystem health assessment, including water quality, water regime, aquatic organism, riparian environment, and physical morphology. Additionally, a set of health assessment system was proposed, including selection of assessment indexes and determination of assessment criteria and health classes. Taking seventeen typical restored urban rivers in Beijing as the assessment target, the said system was applied in assessing urban river health in 2016 and 2019. As the assessment results indicated, in 2016, the health statuses of 29 percent of urban rivers were ordinary, while 71 percent of urban rivers were somewhat inferior. In 2019, the health state of only one urban river reached "good" level. The health statuses of 88 percent of urban rivers were ordinary, and 6 percent were somewhat inferior in terms of comprehensive health index. In 2019, the health states of rivers improved significantly compared with that of 2016, which indicated that most urban rivers saw marked improvement in ecosystem health after ecological restoration. The health assessment system proposed in the paper not only could be applied to regular evaluation of restored urban rivers in the north but also was suitable for a contrastive health-state analysis between different years prior to or after the restoration. In order to carry out adaptive management of water ecology in urban rivers, the measures of ecological restoration could be adjusted based on the regular health assessment and health weakness analysis.
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http://dx.doi.org/10.3390/ijerph19095619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105896PMC
May 2022

DNA Kirigami Driven by Polymerase-Triggered Strand Displacement.

Small 2022 Jun 13;18(24):e2201478. Epub 2022 May 13.

School of Artificial Intelligence and Automation, Huazhong University of Science and Technology, Wuhan, 430074, China.

The precursors of functional biomolecules in living cells are synthesized in a bottom-up manner and subsequently activated by modification into a delicate structure with near-atomic precision. DNA origami technology provides a promising way to mimic the synthesis of precursors, although mimicking the modification process is a challenge. Herein, a DNA paper-cutting (DNA kirigami) method to trim origami into designer nanostructures is proposed, where the modification is implemented by a polymerase-triggered DNA strand displacement reaction. Six geometric shapes are created by cutting rectangular DNA origami. Gel electrophoresis and atomic force microscopy results demonstrate the feasibility and capability of the DNA paper-cutting method. The proposed DNA paper-cutting strategy can enrich the toolbox for dynamically transforming DNA origami and has potential applications in biomimetics. .
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http://dx.doi.org/10.1002/smll.202201478DOI Listing
June 2022

Development of a Novel Immune-Related Gene Prognostic Index for Breast Cancer.

Front Immunol 2022 26;13:845093. Epub 2022 Apr 26.

Innovative Institute of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.

Objective: To construct an immune-related gene prognostic index (IRGPI) for breast cancer (BC) and investigate its prognostic specificity and the molecular and immune characteristics.

Methods: BC hub genes were identified from The Cancer Genome Atlas and immune-related databases using weighted gene co-expression network analysis (WGCNA). IRGPI was constructed using univariate, LASSO, and multivariate regression analyses, and was validated with GSE58812 and GSE97342 in the Gene Expression Omnibus database (GEO). At the same time, we evaluated the predictive ability of IRGPI for different BC subtypes. Subsequently, the molecular and immune characteristics, clinical relevance, and benefits of immune checkpoint inhibitor treatment were analyzed for different IRGPI subgroups.

Results: IRGPI consisted of six genes: SOCS3, TCF7L2, TSLP NPR3, ANO6, and HMGB3. The IRGPI 1-, 5-, and 10-years area under curve (AUC) values were 0.635, 0.752, and 0.753, respectively, indicating that IRGPI has good potential in predicting the long-term survival of BC patients, consistent with the results in the GEO cohort. IRGPI showed good predictive power in four different breast cancer subtypes: ER positive, PR positive, HER2 positive and triple-negative (<0.01). Compared with the low-IRGPI group, the high-IRGPI group had a worse prognosis and a lower degree of immune infiltrating cells ( < 0.05). IRGPI showed specificity in distinguishing age, TNM stage, ER, and HER2 statuses, and our study found that the high-IRGPI group had low tumor immune dysfunction and exclusion (TIDE), microsatellite instability (MSI), and T cell dysfunction scores ( < 0.05). In addition, compared with the TIDE and TIS models, showed that the AUCs of IRGPI were better during the 5-year follow-up.

Conclusion: IRGPI can be used as an independent prognostic indicator of breast cancer, providing a method for monitoring the long-term treatment of BC.
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http://dx.doi.org/10.3389/fimmu.2022.845093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086776PMC
April 2022

Highly Durable Ether-Free Polyfluorene-Based Anion Exchange Membranes for Fuel Cell Applications.

ACS Macro Lett 2021 Oct 14;10(10):1180-1185. Epub 2021 Sep 14.

Institute of Intelligent Flexible Mechatronics, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

The preparation of anion exchange membranes (AEMs) with excellent chemical and dimensional stability and high conductivity faces several challenges. In the present work, a novel ether-free durable polyfluorene (PF) without fluorine-bearing pendant piperidinium groups was synthesized by the Suzuki cross-coupling reaction. Alkyl groups were introduced into the backbone of PF to enhance the solubility and flexibility of PF-based AEMs, and the transparent and flexible polymer membrane showed a high conductivity of 80.44 mS cm and excellent alkaline stability in 2 M KOH solution at 80 °C. Although the membrane possesses a high ion exchange capacity (IEC) (2.49 mequiv g), it exhibits a low swelling ratio (9.4% at 80 °C), excellent mechanical properties, and dimensional stability. The H/O single cell assembled with PFPE-Pi exhibited a maximum power density of 661 mW cm at a current density of 1280 mA cm at 80 °C. The present work provides a simple and effective strategy for the preparation of ether-free polyfluorene-based AEMs with high conductivity, excellent mechanical properties, and dimensional stability for application in alkaline fuel cells.
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http://dx.doi.org/10.1021/acsmacrolett.1c00506DOI Listing
October 2021

Changes and closure grades of the laryngeal vestibule in different positions after anesthesia induction.

Minerva Anestesiol 2022 May 13. Epub 2022 May 13.

Department of Anesthesiology, Yan'an Branch of Peking University Third Hospital, Yan'an City, Shanxi Province, China -

Background: To observe changes in the laryngeal vestibule under head extension or in neutral position with or without the chin lifted after anesthesia induction.

Methods: After anesthesia induction, a flexible bronchoscope was used to observe the status of the laryngeal vestibule under four positions. Then, the degree of laryngeal vestibule closure was graded. The grading scores and the incidence of severe closure under these four positions were compared. The factors that may be related to the severe closure and potential contributions of head extension and chin lifting were also analyzed. When laryngeal mask was applied, the change in the laryngeal vestibule state was observed through the laryngeal mask after placement.

Results: The closure scores of the laryngeal vestibule were significantly different among these four positions (P<0.001). The incidence of severe closure was highest in the neutral head position without the chin lifted. Regression analysis showed that age and snoring were associated with severe closure of the laryngeal vestibule. Both head extension and chin lifting could decrease the risk of severe closure of the laryngeal vestibule, and head extension was more important than chin lift. After laryngeal mask placement, the severely closed laryngeal vestibule can be further opened up to grades 1-2.

Conclusions: The neutral position of the head after anesthesia induction may cause complete closure of the laryngeal vestibule. The best way to keep the laryngeal vestibule open is head extension with the chin lifted, while most the laryngeal vestibules will be severely closed in the neutral position without the chin lifted.
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http://dx.doi.org/10.23736/S0375-9393.22.16438-2DOI Listing
May 2022
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