Publications by authors named "Fei Xie"

444 Publications

Contralateral axillary lymph node metastasis and molecular changes in second primary breast cancer: a case report.

Gland Surg 2021 Apr;10(4):1547-1552

Breast Disease Department, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Contralateral axillary metastasis (CAM) is rather rare in primary breast cancer. In this case, we present a 46-year-old female patient who underwent left breast-conserving surgery (BCS) and left axillary lymph node dissection (ALND). Two years later, an enlarged lymph node was found in her right axilla. Magnetic resonance imaging (MRI) of the breast displayed a left breast mass with multiple internal mammary lymph nodes and abnormal lymph nodes in the right axillary region. However, no abnormalities were found in the right breast. The left breast mass was diagnosed as invasive carcinoma by core needle biopsy. During the operation, we suggested that the contralateral lymph nodes were metastatic from the second primary breast cancer by preoperative 99mTc injection around the left breast. The patient underwent left mastectomy and right axillary lymph node dissection. The postoperative pathology was diagnosed as metachronous secondary primary left breast cancer, in which the initial presentation was lymph node metastasis to the contralateral axilla of the left breast. Therefore, we propose that CAM may be more common in second primary or recurrent breast cancer. It should be treated as locoregional extension. Preoperative lymph node markers are important to identify whether contralateral axillary lymph node metastasis occurs from a second primary breast cancer.
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http://dx.doi.org/10.21037/gs-20-708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102228PMC
April 2021

Motor expertise and performance in sport-specific priming tasks: a systematic review and meta-analysis.

PeerJ 2021 13;9:e11243. Epub 2021 Apr 13.

School of Psychology, Shanghai University of Sport, Shanghai, China.

Objective: The present study aimed to summarize findings relevant to the influence of motor expertise on performance in sport-specific priming tasks and to examine potential moderators of this effect.

Methodology: Data were collected from the China National Knowledge Infrastructure (CNKI), PsychInfo, Medline, Google Scholar, Web of Science, Baidu Scholar and Sport Discus and Dissertation Abstracts Online databases from January 1999 to April 2020, supplemented by manual bibliographies and meeting minutes. Stata software was used to perform the meta-analysis. Study quality was evaluated systematically using the Newcastle-Ottawa scale (NOS). Standard mean differences (SMDs) with 95% CIs were calculated with a random-effects model. The Cochrane test and statistic were used to evaluate heterogeneity. Begg funnel plots and Egger tests were conducted to assess publication bias.

Results: Nine articles (including 12 studies) were ultimately included in the meta-analysis. Significant heterogeneity was observed among these studies ( = 44.42, < 0.001, = 75.2%) according to random-effects modeling. The results showed an overall advantage in favor of motor experts in sport-specific priming tasks (SMD = -1.01, 95% CI [-1.41 to -0.61]). However, the magnitude of that effect was moderated by sport type (interceptive sports/independent sports) and prime stimulus type (subliminal stimulus/supraliminal stimulus). No publication bias was detected by the Begg and Egger tests.

Conclusions: In general, compared with those of nonexperts, the responses of motor experts' responses to a target stimulus are easier and faster when the prime and target stimuli are consistent. However, the magnitude of this effect is moderated by sport type and prime stimulus type.
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http://dx.doi.org/10.7717/peerj.11243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051356PMC
April 2021

Clinical practice guidelines for diagnosis and treatment of invasive breast cancer: Chinese Society of Breast Surgery (CSBrS) practice guidelines 2021.

Chin Med J (Engl) 2021 Apr 28;134(9):1009-1013. Epub 2021 Apr 28.

Department of Breast Center, Peking University People's Hospital, Beijing 100044, China.

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http://dx.doi.org/10.1097/CM9.0000000000001498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116006PMC
April 2021

Inhalable Jojoba Oil Dry Nanoemulsion Powders for the Treatment of Lipopolysaccharide- or HO-Induced Acute Lung Injury.

Pharmaceutics 2021 Apr 2;13(4). Epub 2021 Apr 2.

Graduates Department, Anhui Medical University, Hefei 230032, China.

Jojoba (Simmondsia chinensis (Link) C.K. Schneid) is a dioecious plant in desert and semi-desert areas, e.g., the Ismailia Desert in Egypt. Jojoba oil (JJBO) is a natural slight yellow oil with the functions of skin barrier repairing and wound healing, which is dermally applied as a traditional medication or cosmetic in the Middle East. The objective of this study was to prepare JJBO dry nanoemulsion powders (JNDs) and investigate their anti-acute lung injury effects. JJBO nanoemulsions (JNEs) were prepared and then lyophilized to JNDs and the properties and simulated lung deposition were measured. Rat acute lung injury (ALI) models were established after intratracheal (i.t.) administration of lipopolysaccharide (LPS) or hydrogen peroxide (HO). JNDs and dexamethasone (DXM) solutions were also i.t. administered to the rats. The pathological states of lung tissues were checked. Inflammatory and oxidative factors in the lung tissues were determined using ELISA methods. NF-κB p65 and caspase-3 were measured with a Western blotting method and an immunohistochemical method, respectively. JNDs had an appropriate mass median aerodynamic diameter (MMAD) of 4.17 µm and a fine particle fraction (FPF) of 39.11%. JNDs showed higher anti-inflammatory effect on LPS-induced ALI than DXM with a decrease in total protein content and down-regulation of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and NF-κB p65. JNDs also showed higher anti-inflammatory and anti-oxidation effect on HO-induced ALI than DXM with elimination of reactive oxygen species (ROS), increasing of superoxide dismutase (SOD), decrease in of lipid peroxide malondialdehyde (MDA) and glutathione (GSH), and inhibition of caspase-3 expression. Moreover, i.t. JNDs attenuated bleeding and infiltrations of the inflammatory cells in the two ALI models. JNDs are a promising natural oil-contained inhalable medication for the treatment of LPS- or HO-induced ALI.
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http://dx.doi.org/10.3390/pharmaceutics13040486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065502PMC
April 2021

Obstructive ureteric calculus with superimposed infections causing reversible posterior leukoencephalopathy syndrome: A case report.

Medicine (Baltimore) 2021 Apr;100(16):e25589

Department of Medicine, Princefield University, P.O. Box MA 128, Ho-Volta Region, Ghana, West Africa.

Rationale: Reversible posterior leukoencephalopathy syndrome (RPLS) is a clinicoradiological phenomenon first observed 2 decades ago. Reversibility is the hallmark of this rare clinical phenomenon once the triggering pathology is aptly and adequately treated. Tinnitus preceding bilateral hearing loss as a symptomatology of RPLS has not been reported in the literature. Furthermore, chronic obstructive ureteric calculus with superimposed infections as a cause of RPLS has not been reported in the literature.

Patient Concerns: A 57-year-old female was admitted at our facility because of 2 days history of hearing loss in both ears. She experienced tinnitus in both ears 2 weeks prior to the hearing loss. She is a known hypertensive. She has also undergone multiple surgical treatments for urinary calculi.

Diagnosis: Computed tomography (CT) scan of the urinary system revealed a calculus at the right ureter. Magnetic resonance imaging (MRI) showed abnormal signals at both temporo-parieto-occipital (TPO) cortices, the subcortical area, as well as the left hippocampus which was consistent with the diagnosis of RPLS.

Interventions: While on antibiotics for treatment of infections, the patient went into hypertensive encephalopathy and pneumonia was also established necessitating intensive care.

Outcomes: We observed a resolution of the patient's temperature and hypertension when the right ureteric stone finally descended into the bladder. Also, we observed disappearance of the abnormal signals at both TPO cortices, the subcortical area, as well as the left hippocampus. Two years follow-up revealed no recurrence of her symptomatology.

Lesions: Patients who present with hypertensive encephalopathy maybe more prone to developing RPLS. Renal insufficiency alone or hypertension alone may not be single predisposing entities to RPLS but rather multiple predisposing factors.
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http://dx.doi.org/10.1097/MD.0000000000025589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078273PMC
April 2021

The clinical prognostic value of lncRNA LINC00675 in cancer patients: A meta-analysis.

Medicine (Baltimore) 2021 Apr;100(16):e25244

Department of Hepatic-Biliary-Pancreatic Surgery, The First People's Hospital of Neijiang, Neijiang, Sichuan, China.

Abstract: A newly discovered long non-coding RNA (lncRNA) is associated with the progression of a variety of tumors. The purpose of this meta-analysis is to explore further the relationship between clinicopathological features and the prognostic value of LINC00675 in caners.We searched the various database, including PubMed, Web of Science, Cochrane Library, Embase together with Wanfang, and China National Knowledge Infrastructure for articles on LINC00675 and clinicopathological characteristics and prognosis of patients with cancers before February 20, 2020. According to the inclusion and exclusion criteria, the studies that meet the criteria were systematically collected through search keywords. The Newcastle Ottawa document quality assessment system was used to evaluate the quality of documents. The required data from literature were extracted, and the hazard ratio (HR), odds ratio (OR), and 95confidence interval (CI) were calculated using stata12.0 software and RevMan5.3 software.A total of 5 studies covering 462 patients were included in this meta-analysis to evaluate the prognostic value of LINC00675 in cancers. Our results showed that high LINC00675 expression was significantly correlated with poor overall survival (OS) (HR = 1.60, 95% CI: 1.23-2.08, P = .0005). Additionally, upregulated expression of LINC00675 was significantly associated with tumor node metastasis stage (OR = 1.74, 95% CI: 1.18-2.58, P = .006) and distant metastasis (OR = 2.22, 95% CI: 1.21-4.08, P = .01).Our study suggests that LINC00675 could be used as a biomarker to evaluate the prognosis of cancer patients. More studies to further confirm that the clinical value of LINC00675 in cancers will be required.
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http://dx.doi.org/10.1097/MD.0000000000025244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078383PMC
April 2021

Application of the ACOSOG Z0011 criteria to Chinese patients with breast cancer: a prospective study.

World J Surg Oncol 2021 Apr 20;19(1):128. Epub 2021 Apr 20.

Breast Center, Peking University People's Hospital, Beijing, China.

Background: Although the ACOSOG Z0011 study showed that axillary lymph node dissection (ALND) could be avoided in a specific population of sentinel lymph node-positive patients, it is not widely accepted by Chinese surgeons. We conducted a prospective single-arm study to confirm whether or not the results of Z0011 are applicable to Chinese patients.

Methods: Patients conforming to the Z0011 criteria were prospectively enrolled at the Peking University People's Hospital Breast Center from November 2014 to June 2019. The clinicopathological features of the study group were compared with those of the Z0011 study group. Lymphedema after surgery, the incidence of local-regional recurrence, and survival were analyzed.

Results: One hundred forty-two patients who met the Z0011 eligibility criteria were enrolled in this study; 115 underwent sentinel lymph node biopsy (SLNB) alone. Compared with the Z0011 trial, younger patients were included (median age, 52 [26-82] years vs 54 [25-90] years; P = 0.03). For clinical T stage, tumor histology, hormone status, lymphovascular invasion, and the number of positive sentinel lymph nodes (SLNs), no statistically significant differences were observed. More patients received adjuvant chemotherapy and endocrine therapy in this study (90.85% vs 58.0% and 80.99% vs 46.6% respectively, P <0.001). A similar percentage of patients received radiotherapy, but more nodal radiotherapy procedures were carried out in our study (54.5% vs 16.9%). After a median follow-up of 29 months, only 1 patient (0.9%) had ipsilateral breast tumor recurrence, and no regional recurrence occurred.

Conclusion: Our study showed that it is achievable to avoid ALND in patients eligible for Z0011 in China.

Trial Registration: ClinicalTrials.gov. Registration number NCT03606616 . Retrospectively registered on 31 July 2018.
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http://dx.doi.org/10.1186/s12957-021-02242-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059271PMC
April 2021

Stereoelectroencephalography-guided radiofrequency thermocoagulation for hypothalamic hamartoma: Electroclinical patterns and the relationship with surgical prognosis.

Epilepsy Behav 2021 May 16;118:107957. Epub 2021 Apr 16.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Department of Stereotactic and Functional Neurosurgery, Beijing Neurosurgical Institute, Beijing, China. Electronic address:

Background: Radiofrequency thermocoagulation (RFTC) guided by stereo-electroencephalography (SEEG) has been proved to be a safe method to reduce seizure frequency in patients with drug-resistant epilepsy. However, there are few reports addressing the value and safety of this procedure in hypothalamic hamartoma (HH).

Objective: To present the results of our experience using SEEG-guided RFTC in HH patients with drug-resistant epilepsy, and identify outcome predictors.

Methods: We retrospectively reviewed the clinical and surgical characteristics of 27 HH-related patients with epilepsy in our center between 2015 and 2019. All patients underwent invasive recordings with SEEG before RFTC was performed. We reported surgical outcome predictors and postoperative follow-up concerning safety and efficacy (mean follow-up, 27.3 months; range, 12-63). Surgical strategy was also analyzed.

Results: Nineteen patients (70.4%) achieved Engel's class I outcome, while 4 patients (14.8%) did not show significant improvement. Of all observed seizures, two different onset patterns of intracranial electrophysiology recorded by SEEG were observed. Patients presented with focal low-voltage fast activity were more likely to obtain seizure freedom (p = 0.045), while classification (p = 0.478), volume (p = 0.546), history of resection (p = 0.713), seizure types (p = 0.859), or seizure duration (p = 0.415) showed no significant effect on the outcome. Weight gain was the most common long-term complication (18.5%).

Conclusion: The SEEG can guide the ablation of HH and serve as an important factor to predict favorable seizure outcomes. Radiofrequency thermocoagulation guided by SEEG can offer a minimally invasive and low-risk surgical approach with excellent outcomes. Disconnecting the attachment of HH should be the appropriate strategy to obtain the best seizure outcome.
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http://dx.doi.org/10.1016/j.yebeh.2021.107957DOI Listing
May 2021

Halogen-Bonded Organic Framework (XOF) Based on Iodonium-Bridged N···I+···N Interactions: A Type of Diphase Periodic Organic Network.

Angew Chem Int Ed Engl 2021 Apr 19. Epub 2021 Apr 19.

Wuhan University, The Institute of Advanced Studies, 299 Bayi Road, 430072, Wuhan, CHINA.

Due to the fascinating structures and wide applications, porous materials with open frameworks have attracted more and more attentions. Herein, a novel two-dimensional (2D) halogen-bonded organic framework ( XOF-TPPE ) was successfully designed and fabricated by iodonium-bridged N···I + ···N interactions between pyridyl groups and I + for the first time. The formation of XOF-TPPE and its linear analogue was monitored by 1 H NMR, UV-Vis, X-ray photoelectron spectroscopy (XPS), IR, SEM, TEM, HRTEM and selected-area electron diffraction (SAED). The structural model of XOF-TPPE was established based on powder X-ray diffraction (PXRD) data and theoretical simulations. Significantly, synchrotron small-angle X-ray scattering (SAXS), DLS and UV-vis spectroscopy experiments suggested that XOF-TPPE still maintains a stable 2D framework structure in solutions. This research opens up a novel avenue for the development of organic frameworks materials, and may bring new promising applications for the field of porous materials.
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http://dx.doi.org/10.1002/anie.202102448DOI Listing
April 2021

Seeking sustainable development policies at the municipal level based on the triad of city, economy and environment: evidence from Hunan province, China.

J Environ Manage 2021 Apr 15;290:112554. Epub 2021 Apr 15.

State Key Laboratory of Remote Sensing Science, Jointly Sponsored by Beijing Normal University and Institute of Remote Sensing and Digital Earth of Chinese Academy of Sciences, Beijing, 100875, China; Beijing Engineering Research Center for Global Land Remote Sensing Products, Institute of Remote Sensing Science and Engineering, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.

Keeping urbanization, economy and eco-environment in harmony is a core issue for attaining Sustainable Development Goals (SDGs) in any complex geographical regions. Previous studies mainly focused on seeking the balance between urban expansion levels, eco-environment quality and socioeconomic degree. But the challenges still exist in solving the negative influence of urban expansion that affects eco-environmental and economic development. Based on the Environmental Kuznets Curve theory, we involved inclusive indexes to analyze the interlinkages of eco-environment quality, economic level, and urban expansion degree, which closely relate to urban sustainable development goals and spatial complexity, as well as using available data corresponding to waterfront cities. Cities in Hunan were taken as a study-case, and the study period of 2006-2016 covers the last 10 years of the millennium development goals agenda and the first 2 years of SDGs agenda. The key indicators of city-economy-environment relationships were different at the provincial level, urban level and urbanization grade. According to the regression models and inverted N shape curve, urban expansion resulted in high positive effects on economic development level and negative effects on ecological environment quality, partically higher at high urbanization level than that of the low ones. But the overall trends were that the environmental quality of the cities was undergoing slowly improving processes both at low and high urban expansion levels. Promoting adaptations with the eco-environmental capacity when formulating policies and taking actions is necessary for realizing sustainable cities and communities (SDG11), life on land (SDG15), decent work and economic growth (SDG8) and responsible consumption and production (SDG12) at the same time. Regulating citizens' density, urban expansion speed in area, the quantity of enterprises with heavy pollution, and the structure of industry to the suitable urbanization stages is an important way for achieving SDGs at provincial and municipal levels.
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http://dx.doi.org/10.1016/j.jenvman.2021.112554DOI Listing
April 2021

Clinical practice guideline for breast fibroadenoma: Chinese Society of Breast Surgery (CSBrS) practice guideline 2021.

Chin Med J (Engl) 2021 Apr 15;134(9):1014-1016. Epub 2021 Apr 15.

Department of Breast Center, Peking University People's Hospital, Beijing 100044, China.

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http://dx.doi.org/10.1097/CM9.0000000000001462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116029PMC
April 2021

Clinical metagenomics assessments improve diagnosis and outcomes in community-acquired pneumonia.

BMC Infect Dis 2021 Apr 15;21(1):352. Epub 2021 Apr 15.

Chinese People's Liberation Army General Hospital, Beijing, 100039, China.

Background: Identifying the causes of community-acquired pneumonia (CAP) is challenging due to the disease's complex etiology and the limitations of traditional microbiological diagnostic methods. Recent advances in next generation sequencing (NGS)-based metagenomics allow pan-pathogen detection in a single assay, and may have significant advantages over culture-based techniques.

Results: We conducted a cohort study of 159 CAP patients to assess the diagnostic performance of a clinical metagenomics assay and its impact on clinical management and patient outcomes. When compared to other techniques, clinical metagenomics detected more pathogens in more CAP cases, and identified a substantial number of polymicrobial infections. Moreover, metagenomics results led to changes in or confirmation of clinical management in 35 of 59 cases; these 35 cases also had significantly improved patient outcomes.

Conclusions: Clinical metagenomics could be a valuable tool for the diagnosis and treatment of CAP.

Trial Registration: Trial registration number with the Chinese Clinical Trial Registry: ChiCTR2100043628 .
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http://dx.doi.org/10.1186/s12879-021-06039-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047593PMC
April 2021

The complete mitochondrial genome of long-tailed red-toothed shrew ( and implication of phylogenetic status.

Mitochondrial DNA B Resour 2021 Mar 31;6(4):1308-1309. Epub 2021 Mar 31.

College of Life Sciences, Sichuan Normal University, Chengdu, China.

We determined a complete mitochondrial genome of . This mitogenome is a circular molecule with 16,838 bp in length, containing 13 protein-coding genes, 22 transfer RNA genes (tRNA), and two ribosomal RNA genes (rRNA). This mitochondrial genome has a base composition of 32.8% A, 29.2% T, 24.9% C, and 13.1% G. We reconstructed Bayesian phylogenetic tree by taking advantage of 19 species belonging to subfamily Soricinae. Phylogenetic tree shows that the long-tailed red-toothed shrew belongs to genus , and it is the closest relationship with This mitochondrial genome provides an important resource for addressing taxonomic issues and studying molecular evolution.
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http://dx.doi.org/10.1080/23802359.2021.1907798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018343PMC
March 2021

CircPTPRA blocks the recognition of RNA N-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression.

Mol Cancer 2021 04 14;20(1):68. Epub 2021 Apr 14.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Circular RNAs (circRNAs) have been found to have significant impacts on bladder cancer (BC) progression through various mechanisms. In this study, we aimed to identify novel circRNAs that regulate the function of IGF2BP1, a key mA reader, and explore the regulatory mechanisms and clinical significances in BC.

Methods: Firstly, the clinical role of IGF2BP1 in BC was studied. Then, RNA immunoprecipitation sequencing (RIP-seq) analysis was performed to identify the circRNAs interacted with IGF2BP1 in BC cells. The overall biological roles of IGF2BP1 and the candidate circPTPRA were investigated in both BC cell lines and animal xenograft studies. Subsequently, we evaluated the regulation effects of circPTPRA on IGF2BP1 and screened out its target genes through RNA sequencing. Finally, we explored the underlying molecular mechanisms that circPTPRA might act as a blocker in recognition of mA.

Results: We demonstrated that IGF2BP1 was predominantly binded with circPTPRA in the cytoplasm in BC cells. Ectopic expression of circPTPRA abolished the promotion of cell proliferation, migration and invasion of BC cells induced by IGF2BP1. Importantly, circPTPRA downregulated IGF2BP1-regulation of MYC and FSCN1 expression via interacting with IGF2BP1. Moreover, the recognition of mA-modified RNAs mediated by IGF2BP1 was partly disturbed by circPTPRA through its interaction with KH domains of IGF2BP1.

Conclusions: This study identifies exonic circular circPTPRA as a new tumor suppressor that inhibits cancer progression through endogenous blocking the recognition of IGF2BP1 to mA-modified RNAs, indicating that circPTPRA may serve as an exploitable therapeutic target for patients with BC.
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http://dx.doi.org/10.1186/s12943-021-01359-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045402PMC
April 2021

Mineralosphere Microbiome Leading to Changed Geochemical Properties of Sedimentary Rocks from Aiqigou Mud Volcano, Northwest China.

Microorganisms 2021 Mar 9;9(3). Epub 2021 Mar 9.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

The properties of rocks can be greatly affected by seepage hydrocarbons in petroleum-related mud volcanoes. Among them, the color of sedimentary rocks can reflect the changes of sedimentary environment and weathering history. However, little is known about the microbial communities and their biogeochemical significance in these environments. In this study, contrasting rock samples were collected from the Aiqigou mud volcano on the southern margin of the Junggar Basin in Northwest China as guided by rock colors indicative of redox conditions. The physicochemical properties and mineral composition are similar under the same redox conditions. For example, the content of chlorite, muscovite, quartz, and total carbon were higher, and the total iron was lower under reduced conditions compared with oxidized environments. High-throughput sequencing of 16S rRNA gene amplicons revealed that different functional microorganisms may exist under different redox conditions; microbes in oxidized conditions have higher diversity. Statistical analysis and incubation experiments indicated that the microbial community structure is closely related to the content of iron which may be an important factor for color stratification of continental sedimentary rocks in the Aiqigou mud volcano. The interactions between organics and iron-bearing minerals mediated by microorganisms have also been hypothesized.
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http://dx.doi.org/10.3390/microorganisms9030560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998385PMC
March 2021

Novel antibody-drug conjugate with UV-controlled cleavage mechanism for cytotoxin release.

Bioorg Chem 2020 Nov 16;111:104475. Epub 2020 Nov 16.

National Engineering Research Center for the Emergency Drug, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. Electronic address:

Antibody-drug conjugates (ADCs) are being developed worldwide with the potential to revolutionize current cancer treatment strategies. However, off-target toxicity caused by the instability of linkers remains one of the main issues to be resolved. Developing a novel photocontrol-ADC with good stability and photocontrolled release seemed to be an attractive and practical solution. In this study, we designed, for the first time, a novel ultraviolet (UV) light-controlled ADC by carefully integrating the UV-cleavable o-nitro-benzyl structure into the linker. Our preliminary work indicated that the ADC exhibited good stability and photocontrollability while maintaining a targeting effect similar to that of the naked antibody. Upon irradiation with UV light, the ADC rapidly released free cytotoxins and exerted significant cytotoxicity toward drug-resistant tumor cells. Compared to those of the unirradiated cells, the EC values of ADCs increased by up to 50-fold. Furthermore, our research confirmed that the degradation products of unirradiated ADC, Cys-1a, were relatively less toxic, thus potentially reducing the off-target toxicity caused by nonspecific uptake of ADCs. The novel design strategy of UV light-controlled ADCs may provide new perspectives for future research on ADCs and promote the development of photocontrol systems.
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http://dx.doi.org/10.1016/j.bioorg.2020.104475DOI Listing
November 2020

The complete mitochondrial genome of white-tailed mole ().

Mitochondrial DNA B Resour 2021 Mar 19;6(3):1112-1113. Epub 2021 Mar 19.

College of Life Sciences, Sichuan Normal University, Chengdu, PR China.

The white-tailed mole () belongs to genus , which is a monotypic genus distributed across Southwestern China, Assam (India), Bengal, and Northern Burma, and Laos. In this study, we obtained the complete mitochondrial genome of . The genome is total 16,875 bp in length, containing 13 protein-coding genes, 22 transfer genes (tRNA), two ribosomal genes (rRNA), and two non-coding regions, with a base composition of 33.5% A, 26.4% T, 25.7% C, and 14.3% G. The nucleotide sequence data of 13 protein-coding genes of and other nine Eulipotyphla species were used to reconstruct a Bayesian phylogenetic tree. The tree shows that belongs to subfamily Talpinae and is closely related to .
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http://dx.doi.org/10.1080/23802359.2021.1899871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995813PMC
March 2021

Evaluating and Balancing the Risk of Breast Cancer-Specific Death and Other Cause-Specific Death in Elderly Breast Cancer Patients.

Front Oncol 2021 12;11:578880. Epub 2021 Mar 12.

Breast Center, Peking University People's Hospital, Beijing, China.

The dilemma of undertreatment and overtreatment of elderly breast cancer patients is common. This study aimed to investigate clinicopathological features, treatment modalities, and survival in women diagnosed with breast cancer at age 70 years or over, and to assist clinicians in developing individualized treatment plans by balancing the risks of breast cancer-specific death (BCSD) and other cause-specific death (OCSD). This retrospective study included 420 women who were diagnosed with pathologically confirmed invasive breast cancer at age 70 years or older from January 2008 to December 2015 at Peking University People's Hospital (PKUPH). We collected baseline health status, tumor characteristics, treatment choices, and outcomes and created nomograms for clinicians to estimate individualized BCSD and OCSD risk directly. During a median follow-up of 71.5 months (range 2 to 144 months) in patients with stage I-III tumors, breast cancer specific survival (BCSS) was 92.4% (376/407) and overall survival (OS) was 78.1% (318/407). There were 89 deaths, and 65.2% (58/89) were non-breast cancer related. Upon multivariate analysis by Cox regression model, tumor size, positive lymph nodes, Ki-67, and surgery were independent predictors of BCSS, and comorbidities, positive lymph nodes, Ki-67, surgery, and endocrine therapy were independent predictors of OS. Propensity score weighted (PSW) was applied to analyze therapeutic efficacy, and there was BCSS and OS benefit with surgery (both < 0.001), BCSS benefit with chemotherapy ( = 0.029), BCSS and OS benefit with endocrine therapy ( = 0.006 and 0.004), and neither BCSS nor OS benefit with radiotherapy (RT) ( = 0.348 and 0.289). Competing-risk nomograms were developed to estimate cumulative mortality probabilities for BCSD and OCSD for individual patients according to clinicopathologic characteristics and treatments. The calibration curves displayed exceptionally, with C-indexes 0.714 for BCSD and 0.717 for OCSD. Older patients had greater risk of dying from non-breast cancer causes. Surgery, chemotherapy, and endocrine therapy were associated with improved survival. Competing risk nomograms allowed individual assessment of BCSD and OCSD, based on clinicopathological characteristics and treatment options, and can be used as a tool to help in choosing appropriate treatment strategies. This study was approved by the Peking University People's Hospital Research Ethics Board on September 4, 2018.
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http://dx.doi.org/10.3389/fonc.2021.578880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994517PMC
March 2021

Iron-Catalyzed Water Oxidation: O-O Bond Formation via Intramolecular Oxo-Oxo Interaction.

Angew Chem Int Ed Engl 2021 Mar 26. Epub 2021 Mar 26.

Center of Basic Molecular Science (CBMS), Department of Chemistry, Tsinghua University, Beijing, 100084, China.

Herein, we report the importance of structure regulation on the O-O bond formation process in binuclear iron catalysts. Three complexes, [Fe (μ-O)(OH ) (TPA) ] (1), [Fe (μ-O)(OH ) (6-HPA)] (2) and [Fe (μ-O)(OH ) (BPMAN)] (3), have been designed as electrocatalysts for water oxidation in 0.1 M NaHCO solution (pH 8.4). We found that 1 and 2 are molecular catalysts and that O-O bond formation proceeds via oxo-oxo coupling rather than by the water nucleophilic attack (WNA) pathway. In contrast, complex 3 displays negligible catalytic activity. DFT calculations suggested that the anti to syn isomerization of the two high-valent Fe=O moieties in these catalysts takes place via the axial rotation of one Fe=O unit around the Fe-O-Fe center. This is followed by the O-O bond formation via an oxo-oxo coupling pathway at the Fe Fe state or via oxo-oxyl coupling pathway at the Fe Fe state. Importantly, the rigid BPMAN ligand in complex 3 limits the anti to syn isomerization and axial rotation of the Fe=O moiety, which accounts for the negligible catalytic activity.
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http://dx.doi.org/10.1002/anie.202100060DOI Listing
March 2021

Long-term follow-up results of fluorescence and blue dye guided sentinel lymph node biopsy in early breast cancer.

Breast Cancer Res Treat 2021 Mar 24. Epub 2021 Mar 24.

Breast Center, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Purpose: This study aimed to assess the efficacy of the combination of indocyanine green (ICG) and methylene blue (MB) dye in early breast cancer patients undergoing sentinel lymph node biopsy (SLNB).

Methods: Between January 2011 and December 2015, 1061 early breast cancer patients underwent SLNB were included. SLNB was performed for enrolled patients by injection of both ICG and MB. Detection rate, positivity, and number of sentinel lymph nodes (SLNs) by ICG and MB were analysed. Axillary recurrence and arm lymphedema at 5.6-year follow-up were reported.

Results: The combination of ICG and MB resulted in a very high detection rate of 99.6%, the median number of sentinel lymph nodes was 3. A total of 374 histologically confirmed positive SLNs were detected in 237 patients, 96.6% of the positive patients and 94.1% of the positive nodes could be identified by the combination of ICG and MB. After a median follow-up of 5.6 (2-9.3) years, 0.64% of patients with negative SLNs had ipsilateral axillary recurrence, and the incidence of arm lymphedema was 2.1%.

Conclusions: Although the missing isotope control weakens the interpretation of the findings, the dual tracing modality of ICG and MB, without involvement of radioactive isotopes, was an effective method for SLNB in patients with early breast cancer.

Trial Registration: ACTRN12612000109808. Registered on 23 January 2012.
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http://dx.doi.org/10.1007/s10549-021-06196-6DOI Listing
March 2021

Novel Compound Heterozygous SACS Mutations in a Case with a Spasticity-Lacking Phenotype of Sacsin-Related Ataxia.

Neurol India 2021 Jan-Feb;69(1):219-221

Department of Neurology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

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http://dx.doi.org/10.4103/0028-3886.310115DOI Listing
March 2021

Progress in Research of Asymptomatic Primary Mitral Regurgitation Examination Methods: A Review of Literature.

Heart Surg Forum 2021 02 15;24(1):E116-E120. Epub 2021 Feb 15.

Department of Cardio-Thoracic Surgery, Chengdu Second People's Hospital, Zunyi Medical University, Chengdu, Sichuan, China.

Mitral regurgitation (MR) is a common valvular heart disease, which can be classified into primary and secondary, according to the cause. Primary mitral regurgitation (PMR) is caused by rheumatic fever, degenerative changes, valve prolapse, etc. The appearance of clinical symptoms has always been the best indicator of surgical intervention in patients with severe PMR, but for asymptomatic patients, the best treatment has been controversial. The choice of follow-up observation or early surgery has different results in different randomized studies. Two-dimensional echocardiography is the most commonly used detection method for evaluating MR, but its evaluation of the degree of reflux may be inaccurate, and there are differences in the outcomes of patients with asymptomatic PMR. Recent studies have shown that three-dimensional echocardiography, cardiac magnetic resonance, speckle-tracking echocardiography, brain natriuretic peptide, and exercise stress test can optimize the timing of surgery for asymptomatic patients and judge the asymptomatic of PMR.
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http://dx.doi.org/10.1532/hsf.3483DOI Listing
February 2021

Lack of Mof reduces acute liver injury by enhancing transcriptional activation of Igf1.

J Cell Physiol 2021 Feb 26. Epub 2021 Feb 26.

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Advanced Medical Research Institute, Shandong University, Qingdao, Shandong, China.

Acute liver injury (ALI) is a rapid pathological process that may cause severe liver disease and may even be life-threatening. During ALI, the function of males absent on the first (MOF) has not yet been elucidated. In this study, we unveiled the expression pattern of MOF during carbon tetrachloride (CCl )-induced ALI and role of MOF in the regulation of liver regeneration. In the process of ALI, MOF is significantly overexpressed in the liver injury area. Knockdown of Mof attenuated CCl -induced ALI, and promoted liver cell proliferation, hepatic stellate cell activation and aggregation to the injured area, and liver fibrosis. Simultaneously, overexpression of Mof aggravated liver dysfunction caused by ALI. By directly binding to the promoter, MOF suppressed the transcriptional activation of Igf1. Knockdown of Mof promotes the expression of Igf1 and activates the Insulin-like growth factor 1 signaling pathway in the liver. Through this pathway, Knockdown of Mof reduces CCl -induced ALI and promotes liver regeneration. Our results provide the first demonstration for MOF contributing to ALI. Further understanding of the role of MOF in ALI may lead to new therapeutic strategies for ALI.
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http://dx.doi.org/10.1002/jcp.30332DOI Listing
February 2021

Combination of albumin-bilirubin grade and clinically significant portal hypertension predicts the prognosis of patients with hepatocellular carcinoma after liver resection.

Biosci Trends 2021 Mar 24;15(1):41-49. Epub 2021 Feb 24.

Department of Liver Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

There is little information concerning whether incorporating clinically significant portal hypertension (CSPH) into albumin-bilirubin (ALBI) grading could improve its predictive capacity. In this study, we investigated the predictive ability of ALBI grade plus CSPH (ALBI-P score) for patients with hepatocellular carcinoma (HCC) after liver resection. Data from 1,679 patients were retrospectively reviewed. The ALBI-P score was calculated from the ALBI grade and a point for CSPH (0 for absence of CSPH and 1 for presence of CSPH). Independent risk factors for recurrence-free survival (RFS) and overall survival (OS) were analyzed. Multivariate analysis suggested that the ALBI-P score was an independent risk factor for both postoperative recurrence (HR = 1.441, 95% CI = 1.328-1.563, P < 0.001) and mortality (HR = 1.332, 95% CI = 1.156-1.535, P < 0.001). Both the RFS and OS of patients with an ALBI-P score of 1 were significantly better than those of patients with ALBI-P scores of 2 and 3 (5-year RFS of 38.9%, 26.1%, and 14.7%, respectively, P < 0.001; 5-year OS of 52.7%, 42.6%, and 29.3%, P < 0.001). When the ALBI-P score and BCLC stage were combined, the ALBI-P-BCLC score showed the highest area under the receiver operating characteristic curve to predict both postoperative recurrence and mortality compared with BCLC stage alone, BCLC stage combined with ALBI grade, or platelet-albumin-bilirubin grade. These results suggested incorporating CSPH into the ALBI grade could strengthen its prognostic power. The ALBI-P score may serve as a surrogate marker to predict HCC patient outcomes after liver resection.
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http://dx.doi.org/10.5582/bst.2021.01064DOI Listing
March 2021

Clinical manifestation and phenotypic analysis of novel gene mutation in 28 Chinese children with hereditary spherocytosis.

Mol Genet Genomic Med 2021 Apr 23;9(4):e1577. Epub 2021 Feb 23.

Department of Pediatrics, Changhai Hospital, Naval Military Medical University, Shanghai, China.

Purpose: Objective to summarize the clinical features and laboratory findings of 28 Chinese children with hereditary spherocytosis (HS), and analyze these mutations.

Method: Collected and analyzed the clinical data of all children and their parents, and completed the relevant laboratory examinations of all children. Analyzed the sequence of related genes by second-generation sequencing technology, and verified the suspected mutations by Sanger sequencing method. Analyzed all biological information using the Single Nucleotide Polymorphism database, the 1000 Human Genome Project, and the Exosome Aggregation Consortium.

Result: New mutations were detected in the HS coding region of 28 children. Among them, there were 13 cases (46.4%) with ANK1 mutation, 10 cases (35.7%) with SPTB mutation, three cases (10.7%) with SLC4A1 mutation, and two cases (7.2%) with SPTA1 mutation. All mutations cause amino acid changes in the coding gene, as well as subsequent changes in protein structure or loss of function.

Conclusion: All the newly discovered gene coding region mutation sites detected are the suspected pathogenic causes of the 28 Chinese children. At the same time, the second-generation gene sequencing technology is an effective means to diagnose HS. Different mutation types and different mutation regions have no significant correlation with the severity of anemia. The novel gene mutation sites in 28 children studied in this paper have not yet been included in the human genome database, dbSNP (v138), or ExAC database. The new gene mutations found in HS children can provide a theoretical basis for further exploring the genetic causes of HS in Chinese children.
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http://dx.doi.org/10.1002/mgg3.1577DOI Listing
April 2021

Mechanical ventilation strategy for pulmonary rehabilitation based on patient-ventilator interaction.

Sci China Technol Sci 2021 Feb 10:1-10. Epub 2021 Feb 10.

Department of Orthopedics, Chinese PLA General Hospital, Beijing, 100039 China.

Mechanical ventilation is an effective medical means in the treatment of patients with critically ill, COVID-19 and other pulmonary diseases. During the mechanical ventilation and the weaning process, the conduct of pulmonary rehabilitation is essential for the patients to improve the spontaneous breathing ability and to avoid the weakness of respiratory muscles and other pulmonary functional trauma. However, inappropriate mechanical ventilation strategies for pulmonary rehabilitation often result in weaning difficulties and other ventilator complications. In this article, the mechanical ventilation strategies for pulmonary rehabilitation are studied based on the analysis of patient-ventilator interaction. A pneumatic model of the mechanical ventilation system is established to determine the mathematical relationship among the pressure, the volumetric flow, and the tidal volume. Each ventilation cycle is divided into four phases according to the different respiratory characteristics of patients, namely, the triggering phase, the inhalation phase, the switching phase, and the exhalation phase. The control parameters of the ventilator are adjusted by analyzing the interaction between the patient and the ventilator at different phases. A novel fuzzy control method of the ventilator support pressure is proposed in the pressure support ventilation mode. According to the fuzzy rules in this research, the plateau pressure can be obtained by the trigger sensitivity and the patient's inspiratory effort. An experiment prototype of the ventilator is established to verify the accuracy of the pneumatic model and the validity of the mechanical ventilation strategies proposed in this article. In addition, through the discussion of the patient-ventilator asynchrony, the strategies for mechanical ventilation can be adjusted accordingly. The results of this research are meaningful for the clinical operation of mechanical ventilation. Besides, these results provide a theoretical basis for the future research on the intelligent control of ventilator and the automation of weaning process.
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http://dx.doi.org/10.1007/s11431-020-1778-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882862PMC
February 2021

Plant egg cell fate determination depends on its exact position in female gametophyte.

Proc Natl Acad Sci U S A 2021 Feb;118(8)

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, 430072 Wuhan, China

Plant fertilization involves both an egg cell, which fuses with a sperm cell, and synergid cells, which guide pollen tubes for sperm cell delivery. Therefore, egg and synergid cell functional specifications are prerequisites for successful fertilization. However, how the egg and synergid cells, referred to as the "egg apparatus," derived from one mother cell develop into distinct cell types remains an unanswered question. In this report, we show that the final position of the nuclei in female gametophyte determines the cell fate of the egg apparatus. We established a live imaging system to visualize the dynamics of nuclear positioning and cell identity establishment in the female gametophyte. We observed that free nuclei should migrate to a specific position before egg apparatus specialization. Artificial changing in the nuclear position on disturbance of the actin cytoskeleton, either in vitro or in vivo, could reset the cell fate of the egg apparatus. We also found that nuclei of the same origin moved to different positions and then showed different cell identities, whereas nuclei of different origins moved to the same position showed the same cell identity, indicating that the final positions of the nuclei, rather than specific nucleus lineage, play critical roles in the egg apparatus specification. Furthermore, the active auxin level was higher in the egg cell than in synergid cells. Auxin transport inhibitor could decrease the auxin level in egg cells and impair egg cell identity, suggesting that directional and accurate auxin distribution likely acts as a positional cue for egg apparatus specialization.
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http://dx.doi.org/10.1073/pnas.2017488118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923378PMC
February 2021

Predicting therapeutic drugs for hepatocellular carcinoma based on tissue-specific pathways.

PLoS Comput Biol 2021 Feb 9;17(2):e1008696. Epub 2021 Feb 9.

Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Advanced Medical Research Institute, Shandong University, 72, Jimo District, Qingdao, Shandong, China.

Hepatocellular carcinoma (HCC) is a significant health problem worldwide with poor prognosis. Drug repositioning represents a profitable strategy to accelerate drug discovery in the treatment of HCC. In this study, we developed a new approach for predicting therapeutic drugs for HCC based on tissue-specific pathways and identified three newly predicted drugs that are likely to be therapeutic drugs for the treatment of HCC. We validated these predicted drugs by analyzing their overlapping drug indications reported in PubMed literature. By using the cancer cell line data in the database, we constructed a Connectivity Map (CMap) profile similarity analysis and KEGG enrichment analysis on their related genes. By experimental validation, we found securinine and ajmaline significantly inhibited cell viability of HCC cells and induced apoptosis. Among them, securinine has lower toxicity to normal liver cell line, which is worthy of further research. Our results suggested that the proposed approach was effective and accurate for discovering novel therapeutic options for HCC. This method also could be used to indicate unmarked drug-disease associations in the Comparative Toxicogenomics Database. Meanwhile, our method could also be applied to predict the potential drugs for other types of tumors by changing the database.
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http://dx.doi.org/10.1371/journal.pcbi.1008696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920387PMC
February 2021

Association between BRCA mutational status and survival in patients with breast cancer: a systematic review and meta-analysis.

Breast Cancer Res Treat 2021 Apr 9;186(3):591-605. Epub 2021 Feb 9.

Breast Center, Peking University, People's Hospital, Beijing, China.

Background: Studies evaluating role of BRCA mutations on the survival outcomes in breast cancer (BC) patients have given confounding results and hence, in this meta-analysis, we assessed the impact of BRCA mutations on survival in BC patients.

Methods: Studies comparing survival outcomes of BC patients having BRCA mutations against wildtype BRCA phenotype were retrieved from PubMed, EMBASE, and Cochrane Library. Overall survival (OS), disease-free survival (DFS), distant metastasis-free survival (DMFS), and breast cancer-specific survival (BCCS) were the outcomes. Hazard ratio (HR) with 95% confidence interval (CI) was used for analysis. Subgroup analysis was performed for survival based on triple negative breast cancer (TNBC) and follow-up durations. The meta-analysis was performed as per PRISMA guidelines.

Results: Altogether, 30 articles with 35,972 patients (mean age 45.6 years) were included. Patients with BRCA 1 mutation had significantly lower OS (HR [95% CI] 1.2 [1.08, 1.33]; P < 0.001), BRCA 2 mutation had significantly lower DFS (HR [95% CI] 1.35 [1.1, 1.67]; P = 0.0049) and BCSS (HR [95%CI] 1.46 [1.26, 1.7]; P < 0.0001), and TNBC patients with BRCA 1 mutation had significantly poor DFS (HR [95% CI] 1.65 [1.08, 2.54]; P = 0.0216). Based on follow-up duration, the OS in BRCA 1-mutated patients revealed significantly poorer outcomes in studies with ≤ 5 years (HR 1.48) and > 5 years (HR 1.14) of follow-up. In BRCA 2 -mutated patients, the OS was significantly poorer in studies with > 5 years of follow-up (HR 1.39, P < 0.05).

Conclusion: BC patients with BRCA 1 or BRCA 2 mutations had poor survival outcomes and hence screening patients with BC for BRCA mutations might help in strategizing their treatment and improving their survival.
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http://dx.doi.org/10.1007/s10549-021-06104-yDOI Listing
April 2021