Publications by authors named "Fei Xia"

250 Publications

Balanced crystalloids versus saline in critically ill patients: The PRISMA study of a meta-analysis.

Medicine (Baltimore) 2021 Sep;100(38):e27203

Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing, China.

Objective: To compare the safety of balanced crystalloids and saline among critically ill patients in intensive care unit (ICU).

Methods: The Medline, EMBASE, Web of Science, Cochrane Library databases were systematically searched from the inception dates to May 17, 2020 in order to identify randomized controlled trials which evaluated the safety of balanced crystalloids and saline in critically ill patients. The primary outcome was major adverse kidney events within 30 days (MAKE30). The second outcomes included 30-day mortality, ICU mortality, In-hospital mortality, ICU length of stay, hospital length of stay, creatinine highest before discharge (mg/dl) and needs for renal replacement therapy (RRT).

Results: A total of nine randomized controlled trials involving 19,578 critical ill patients fulfilled the inclusion criteria. The outcomes of this meta-analysis showed that balanced crystalloids treatment shared the same risk of MAKE30 with saline treatment among critical ill patients [RR = 0.95; 95%CI, 0.88 to 1.01; Z = 1.64 (P = .102)]. The clinical mortality which included 30-day mortality [RR = 0.92; 95%CI, 0.85 to 1.01; Z = 1.78 (P = .075)], ICU mortality [RR = 0.92; 95%CI, 0.83 to 1.02; Z = 1.67 (P = .094)] and In-hospital mortality [RR = 0.93; 95%CI, 0.71 to 1.21; Z = 0.55 (P = .585)] were similar between balanced crystalloids treatment and saline treatment among critical ill patients. Patients who received balanced crystalloids treatment or saline treatment needed the same length of ICU stay [WMD = 0.00; 95%CI, -0.09 to 0.10; Z = 0.09 (P = .932)] and hospital stay [WMD = 0.59; 95%CI, -0.33 to 1.51; Z = 1.26 (P = .209)]. Critical ill patients who received balanced crystalloids treatment or saline treatment had the same level of creatinine highest before discharge [WMD = 0.01; 95%CI, -0.02 to 0.04; Z = 0.76 (P = .446)] and needs for RRT [RR = 1.04; 95%CI, 0.75 to 1.43; Z = 0.21 (P = .830)]. Similar results were obtained in subgroups of trials stratified according to the age of patients (children or adults).

Conclusions: When compared with saline, balanced crystalloids could not reduce the risk of MAKE30, 30-day mortality, ICU mortality and in-hospital mortality, could not reduce the length of ICU stay, length of hospital stay, the level of creatinine highest before discharge and the needs for RRT among critical ill children and adults. Therefore, it was still too early for balanced crystalloids to replace normal saline among critical ill patients.
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http://dx.doi.org/10.1097/MD.0000000000027203DOI Listing
September 2021

Two allergens from Scylla paramamosain share common epitopes showed different allergenic potential in Balb/c mice.

Food Chem 2021 Sep 15;371:131132. Epub 2021 Sep 15.

College of Ocean Food and Biological Engineering, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China. Electronic address:

Filamin C (FLN c) and triosephosphate isomerase (TIM) are novel allergens of crab (Scylla paramamosain) which are sharing common epitopes. This work aimed to assess their contributions to the induction and elicitation of allergenic responses. Balb/c mice were sensitized by intraperitoneal injections and challenged by intragastric gavage with purified proteins. Upon oral challenge, FLN c triggered more severe anaphylactic symptoms, higher levels of specific antibodies and histamine in serum than TIM, while TIM was a more active promotor of early specific antibody production and stimulated stronger Th2-biased responses. Combined with the results of in vitro assays, the data demonstrated that though with common epitopes, the two allergens showed a different allergenicity, TIM favored Th2 polarization in sensitization stage, while FLN c had a better ability to stimulate B cells and is highly immunogenic in oral challenge stage. The findings can help with the better understanding of allergenicity of crab allergens.
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http://dx.doi.org/10.1016/j.foodchem.2021.131132DOI Listing
September 2021

Tagged Highly Degenerate Primer (THDP)-PCR for Community Analysis of Methane- and Ammonia-oxidizing Bacteria Based on Copper-containing Membrane-bound Monooxygenases (CuMMO).

Bio Protoc 2017 Jun 20;7(12):e2354. Epub 2017 Jun 20.

Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.

We describe a two-step PCR strategy using tagged highly degenerate primer (THDP-PCR) targeting copper-containing membrane-bound monooxygenases (CuMMO) genes for community analysis of methane- or ammonia-oxidizing bacteria. This strategy consists of a primary CuMMO gene-specific PCR followed by a secondary PCR with a tag as a single primer. This strategy remarkably increases the divergence of CuMMO gene amplicons while maintaining PCR efficiency without obvious amplification bias. This THDP-PCR strategy can be extended to other functional gene-based community analysis with design of new highly degenerate primer covering target functional gene sequences.
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http://dx.doi.org/10.21769/BioProtoc.2354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410328PMC
June 2017

Closed-loop wavefront sensing and correction in the mouse brain with computed optical coherence microscopy.

Biomed Opt Express 2021 Aug 16;12(8):4934-4954. Epub 2021 Jul 16.

Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.

Optical coherence microscopy (OCM) uses interferometric detection to capture the complex optical field with high sensitivity, which enables computational wavefront retrieval using back-scattered light from the sample. Compared to a conventional wavefront sensor, aberration sensing with OCM via computational adaptive optics (CAO) leverages coherence and confocal gating to obtain signals from the focus with less cross-talk from other depths or transverse locations within the field-of-view. Here, we present an investigation of the performance of CAO-based aberration sensing in simulation, bead phantoms, and mouse brain tissue. We demonstrate that, due to the influence of the double-pass confocal OCM imaging geometry on the shape of computed pupil functions, computational sensing of high-order aberrations can suffer from signal attenuation in certain spatial-frequency bands and shape similarity with lower order counterparts. However, by sensing and correcting only low-order aberrations (astigmatism, coma, and trefoil), we still successfully corrected tissue-induced aberrations, leading to 3× increase in OCM signal intensity at a depth of ∼0.9 mm in a freshly dissected mouse brain.
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http://dx.doi.org/10.1364/BOE.427979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407825PMC
August 2021

Conformational Features of Ras: Key Hydrogen-Bonding Interactions of Gln61 in the Intermediate State during GTP Hydrolysis.

J Phys Chem B 2021 Aug 29;125(31):8805-8813. Epub 2021 Jul 29.

Collaborative Innovation Center of Chemistry for Energy Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, MOE Key Laboratory of Computational Physical Sciences, Departments of Chemistry, Fudan University, Shanghai 200433, China.

The Ras protein is one of the most important drug targets for battling cancers. To effectively design novel drugs of Ras, we characterize here its conformational ensembles for the hydrolysis intermediate state RasGDP·Pi and the product state RasGDP by extensive replica-exchange molecular dynamics simulations. Several substates for RasGDP·Pi have been identified, while structural analyses have revealed an unrecognized hydrogen-bonding network that stabilizes the hydrolysis intermediate state. More interestingly, Gln61, which is involved in numerous oncogenic mutations, was found to be engaged in this hydrogen-bonding network, adopting a specific conformation that always points to Pi in contrast to that in the RasGTP state. The simulations also reveal that RasGDP has more than one substate, suggesting a conformational selection mechanism for the interaction between Ras and the guanine nucleotide exchange factors (GEFs). These findings offer new opportunities for the drug design of Ras by stabilizing the hydrolysis intermediate or disrupting its interaction with the GEFs.
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http://dx.doi.org/10.1021/acs.jpcb.1c04679DOI Listing
August 2021

Vanillic acid combats Vibrio alginolyticus by cell membrane damage and biofilm reduction.

J Fish Dis 2021 Jul 26. Epub 2021 Jul 26.

School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, China.

Antibiotics are the most powerful weapon against bacterial infectious diseases in aquaculture. However, the indiscriminate usage of antibiotics often culminates in the emerging development of antibiotic-resistant bacteria, making it imperative to search for novel types of antimicrobial agents. This study investigated the antibacterial and antivirulence effects of vanillic acid (VA) against the fish pathogen, Vibrio alginolyticus. We showed that VA had a good anti-Vibrio activity with minimal inhibitory concentration (MIC) of 1.0 mg/ml. In addition, VA wielded its antibacterial action in a dose-/time-dependent manner by causing cell membrane damage and increasing membrane permeability, which is evidenced by increasing the conductivity and malondialdehyde content in the treated cell cultures and the scanning electron microscopy images. Furthermore, VA significantly reduced the biofilm-forming capability, mobility and exotoxin production (protease and exopolysaccharide) and downregulation of the expression of biofilm- and virulence-associated genes (sypG, fliS, fliK, lafA, lafK, asp and luxR) was seen in the V. alginolyticus that exposed to VA at subinhibitory concentrations. Overall, our findings suggested that VA may be of interest for treating V. alginolyticus-associated infections in aquaculture.
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http://dx.doi.org/10.1111/jfd.13498DOI Listing
July 2021

Access to Epidemic Information and Life Satisfaction under the Period of COVID-19: the Mediating Role of Perceived Stress and the Moderating Role of Friendship Quality.

Appl Res Qual Life 2021 Jul 1:1-19. Epub 2021 Jul 1.

School of Education, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022 China.

The present study mainly focused on college students amidst the COVID-19 outbreak and aimed to develop and examine a moderated mediation model between access to epidemic information and life satisfaction. Friendship quality as a moderator, and perceived stress as a mediator. A sample of 1032 college students participated in this study and completed questionnaires regarding access to epidemic information, perceived stress, friendship quality, and life satisfaction. Findings indicated that 1) access to epidemic information was strongly related to life satisfaction; 2) perceived stress acts as a mediator in the positive relationship between access to epidemic information and life satisfaction; 3) friendship quality moderated the relationship between access to epidemic information and perceived stress as well as perceived stress and life satisfaction, and such that there was a stronger association between access to epidemic information and perceived stress for college students with high friendship quality. But the relationship between perceived stress and life satisfaction became weaker for college students with high friendship quality. The results illuminate the mechanism to theoretical and practical implications for improving college students' life satisfaction during the pandemic.
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http://dx.doi.org/10.1007/s11482-021-09957-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245917PMC
July 2021

Coronary artery calcification and risk of mortality and adverse outcomes in patients with COVID-19: a Chinese multicenter retrospective cohort study.

Chin J Acad Radiol 2021 Jun 28:1-9. Epub 2021 Jun 28.

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, 305 Zhongshan East Road, Xuanwu District, Nanjing, 210002 Jiangsu Province People's Republic of China.

Background: Coronary artery calcification (CAC) is an independent risk factor of major adverse cardiovascular events; however, the impact of CAC on in-hospital death and adverse clinical outcomes in patients with coronavirus disease 2019 (COVID-19) remains unclear.

Objective: To explore the association between CAC and in-hospital mortality and adverse events in patients with COVID-19.

Methods: This multicenter retrospective cohort study enrolled 2067 laboratory-confirmed COVID-19 patients with definitive clinical outcomes (death or discharge) admitted from 22 tertiary hospitals in China between January 3, 2020 and April 2, 2020. Demographic, clinical, laboratory results, chest CT findings, and CAC on admission were collected. The primary outcome was in-hospital death and the secondary outcome was composed of in-hospital death, admission to intensive care unit (ICU), and requiring mechanical ventilation. Multivariable Cox regression analysis and Kaplan-Meier plots were used to explore the association between CAC and in-hospital death and adverse clinical outcomes.

Results: The mean age was 50 years (SD,16) and 1097 (53.1%) were male. A total of 177 patients showed high CAC level, and compared with patients with low CAC, these patients were older (mean age: 49 vs. 69 years,  < 0.001) and more likely to be male (52.0% vs. 65.0%,  = 0.001). Comorbidities, including cardiovascular disease (CVD) ([33.3%, 59/177] vs. [4.7%, 89/1890],  < 0.001), presented more often among patients with high CAC, compared with patients with low CAC. As for laboratory results, patients with high CAC had higher rates of increased D-dimer, LDH, as well as CK-MB (all  < 0.05). The mean CT severity score in high CAC group was also higher than low CAC group (12.6 vs. 11.1,  = 0.005). In multivariable Cox regression model, patients with high CAC were at a higher risk of in-hospital death (hazard ratio [HR], 1.731; 95% CI 1.010-2.971,  = 0.046) and adverse clinical outcomes (HR, 1.611; 95% CL 1.087-2.387,  = 0.018).

Conclusion: High CAC is a risk factor associated with in-hospital death and adverse clinical outcomes in patients with confirmed COVID-19, which highlights the importance of calcium load testing for hospitalized COVID-19 patients and calls for attention to patients with high CAC.

Supplementary Information: The online version contains supplementary material available at 10.1007/s42058-021-00072-4.
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http://dx.doi.org/10.1007/s42058-021-00072-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237549PMC
June 2021

Metagenomic Analysis of Marigold: Mixed Infection Including Two New Viruses.

Viruses 2021 06 28;13(7). Epub 2021 Jun 28.

College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China.

Marigold plants with symptoms of mosaic, crinkle, leaf curl and necrosis were observed and small RNA and ribo-depleted total RNA deep sequencing were conducted to identify the associated viruses. Broad bean wilt virus 2, cucumber mosaic virus, turnip mosaic virus, a new potyvirus tentatively named marigold mosaic virus (MMV) and a new partitivirus named as marigold cryptic virus (MCV) were finally identified. Complete genome sequence analysis showed MMV was 9811 nt in length, encoding a large polyprotein with highest aa sequence identity (57%) with the putative potyvirus polygonatumkingianum virus 1. Phylogenetic analysis with the definite potyviruses based on the polyprotein sequence showed MMV clustered closest to plum pox virus. The complete genome of MCV comprised of dsRNA1 (1583 bp) and dsRNA2 (1459 bp), encoding the RNA-dependent RNA polymerase (RdRp), and coat protein (CP), respectively. MCV RdRp shared the highest (75.7%) aa sequence identity with the unclassified partitivirus ambrosia cryptic virus 2, and 59.0%, 57.1%, 56.1%, 54.5% and 33.7% with the corresponding region of the definite delta-partitiviruses, pepper cryptic virus 2, beet cryptic virus 3, beet cryptic virus 2, pepper cryptic virus 1 and fig cryptic virus, respectively. Phylogenetic analysis based on the RdRp aa sequence showed MCV clustered into the delta-partitivirus group. These findings enriched our knowledge of viruses infecting marigold, but the association of the observed symptom and the identified viruses and the biological characterization of the new viruses should be further investigated.
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http://dx.doi.org/10.3390/v13071254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310094PMC
June 2021

OmpC, a novel factor H-binding surface protein, is dispensable for the adherence and virulence of Salmonella enterica serovar Typhimurium.

Vet Microbiol 2021 Aug 18;259:109157. Epub 2021 Jun 18.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China; Key Laboratory of Animal Infectious Diseases, Ministry of Agriculture, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address:

Salmonella enterica serovar Typhimurium utilizes a series of strategies to evade host innate immune defenses, including the serum complement system. Many microbial pathogens have evolved the ability to bind the complement regulatory protein factor H (FH) through their surface factor H-binding proteins (FHBPs) to circumvent the complement-mediated bactericidal effect. However, the roles of FHBPs in Salmonella pathogenesis are not well understood. In this study, we demonstrated that the survival of S. Typhimurium in human serum was decreased in a time and concentration dependent manner. Pre-incubation with FH attenuated the sensitivity of S. Typhimurium strain χ3761 to complement-mediated serum killing, suggesting FH binding enhance survival in serum. We aimed to identify novel S. Typhimurium FHBPs and characterize their biological functions. Here, six potential FHBPs were identified by two-dimensional (2D)-Far-western blot, and three of them were further confirmed to bind FH by Far-western blot and dot blot. We found that deletion of ompC (ΔompC) significantly inhibited the survival of S. Typhimurium strain χ3761 in human serum. Our results indicated that the ompC mutation does not affect χ3761 adhesion to HeLa cells. Furthermore, a mice infection model showed that deletion of ompC had no significant effect on the histopathological lesions or viability compared with the wild-type strain χ3761. In summary, these results suggested that OmpC is an important FHBP, but not a critical virulence factor of S. Typhimurium.
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http://dx.doi.org/10.1016/j.vetmic.2021.109157DOI Listing
August 2021

Maternal Immune System and State of Inflammation Dictate the Fate and Severity of Disease in Preeclampsia.

J Immunol Res 2021 5;2021:9947884. Epub 2021 Jun 5.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Soochow University, Soochow, China.

Preeclampsia, a multisystem disorder in pregnant women, is diagnosed by onset of new hypertension, proteinuria, or organ damage. Antiangiogenic factors, such as soluble fms-like tyrosine kinase 1 (sFlt1) and soluble endoglin (sEng), are long known to be involved in preeclampsia. However, the role of maternal immune system and inflammation in promotion of preeclampsia has lately been a subject of immense interest. Link between maternal inflammation and preeclampsia is not well established. Furthermore, whether cigarette smoke promotes inflammation and also promotes severity of preeclampsia remains an open question. We herein investigated correlation of established inflammation signatures in the plasma and placental tissue from cohorts of preterm preeclampsia (PPE) and preterm pregnancies (control) with or without smoking history. Besides confirming increased levels of Flt1 and Eng in preeclampsia, we also observed an increase in various mediators of maternal inflammation in women with PPE compared to preterm cohort. Increased IL-6, IL-35, and TNF- and reduced IL-10 in serum and higher MMP-12, TLR4, HMGB-1, and iNOS and lower Foxp3, CD56 transcripts in placental tissues of PPE compared to preterm pregnancies indicate an association of preterm preeclampsia with stark imbalance in maternal immune system and signatures of inflammation. Smoker PPE cohorts showed highest inflammatory signatures including statistically significant increase for many signatures compared to other cohorts. Together, these results provide evidence for association of inflammation with PPE and strong correlation of smoking with inflammatory signatures in PPE.
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http://dx.doi.org/10.1155/2021/9947884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203389PMC
June 2021

Ammonium promoting methane oxidation by stimulating the Type Ia methane-oxidizing bacteria in tidal flat sediments of the Yangtze River estuary.

Sci Total Environ 2021 Nov 15;793:148470. Epub 2021 Jun 15.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai, China. Electronic address:

Estuary and coastal environments have essential ecosystem functions in greenhouse gas sinks and removal of nitrogen pollution. Methane-oxidizing bacteria (MOB) and ammonia-oxidizing bacteria (AOB) communities play critical functions in the estuary's tidal flat sediments. Therefore, the effects of ammonium on MOB communities and methane on AOB communities need to be further explained. In this study, microcosm incubations with different contents of ammonium or methane were conducted for a relatively short (24 h) or long (28 days) period with tidal flat sediments from the Yangtze River estuary. Subsequently, the tagged highly degenerate primer PCR and DNA-based stable isotope probing method were employed to demonstrate the effects on MOB and AOB populations. The results indicated that the methane consumption was enhanced with ammonium supplements within 24 h of incubation. Supplement of 2 μmol/g d.w.s (μmol per gram dry weight soil) NH increased the amount of MOB and its proportion to the total bacteria (p < 0.05) for 28 days incubation. The ammonium supplement increased the proportion of Methylomonas and Methylobacter based on the 16S rRNA gene. According to the functional gene analysis, the MOB primarily engaged in methane oxidation include Methylomonas, Methylobacter, Methylomicrobium, and Methylosarcina, which were associated with Type Ia MOB. It suggested that ammonium supplement may promote methane oxidation by stimulating the Type Ia MOB in tidal flat sediments of the Yangtze River estuary. The current research helps understand the effect of ammonium on methane consumption in the estuary and coastal environments.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148470DOI Listing
November 2021

Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Shang Qiao Zhong Lu, Chaoyang DistrictBeijing, China, 100024.

To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus (CNSV), apple stem grooving virus (ASGV), lychnis mottle virus (LycMoV), grapevine line pattern virus (GLPV), and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, Citrivirus in Betaflexiviridae), peony betaflexivirus 1 (PeV1, unclassified in Betaflexiviridae), and peony leafroll-associated virus (PLRaV, Ampelovirus in Closteroviridae). PYaCV was 8,666 nucleaotides (nt) in length, comprising three open reading frames (ORFs) and shared 63.8-75.9% nucleotide sequence identity with citrus leaf blotch virus (CLBV) isolates. However, the ORF encoding the replication-associated protein (REP) shared 57% and 52% sequence identities at the nt and amino acid (aa) level, respectively, with other reported CLBV isolates, which were below the criterion for species classification within the family Betaflexiviridae. Recombination analysis identified putative recombination sites in PYaCV, which originated from CLBV. PeV1, only identified from the transcriptome data, was 8,124 nt in length with five ORFs encoding the REP (ORF1), triple gene block (TGB, ORF2-4) and coat protein (CP, ORF5) proteins. Phylogenetic analysis and sequence comparison showed that PeV1 clustered with an unassigned member, the garlic yellow mosaic-associated virus (GYMaV) within the Betaflexiviridae family, into a separate clade. Partial genome sequence analysis of PLRaV (12,545 nt) showed it contained seven ORFs encoding the partial polyprotein 1a, the RNA-dependent RNA polymerase (RdRp), two small hydrophobic proteins p11 and p6, HSP70h, p55, and a CP duplicate, which shared low aa sequence identity with Closteroviridae family members. Phylogenetic analysis based on the aa sequences of RdRp or HSP70h indicated that PLRaV clustered with grapevine leafroll-associated virus 1 (GLRaV-1) and GLRaV-13 in the Ampelovirus genus. Field investigation confirmed the wide distribution of these viruses, causing mixed infections of peony plants in Beijing.
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http://dx.doi.org/10.1094/PDIS-01-21-0007-REDOI Listing
June 2021

New insights into metabolomics profile generation in fermented tea: the relevance of bacteria and metabolites in Fuzhuan brick tea.

J Sci Food Agric 2021 Jun 18. Epub 2021 Jun 18.

School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, China.

Background: The contribution of bacteria to fermented tea is not clear and the associated research is relatively limited. To reveal the role of microorganisms in fermented tea processing, the microbial community and metabolites of Fuzhuan brick tea (FBT), a Chinese traditional fermented tea, were revealed via high-throughput sequencing and liquid chromatography-mass spectrometry (LC-MS).

Results: In FBT, bacterial communities had a higher abundance and diversity, Lactococcus and Bacillus were the main bacteria, and Eurotium was the predominant fungus. The predictive metabolic function indicated the pathways of cellular growth, environmental information, genetics and material metabolism of bacterial communities were abundant, whereas the fungal community predictive metabolic function was almost saprotroph. Using LC-MS, 1143 and 536 metabolites were defined in positive and negative ion mode, respectively. There were essential correlations between bacterial populations and metabolites, such that Bacillus was correlated significantly with 44 metabolites (P < 0.05) and Enterococcus was significantly associated with 15 metabolites (P < 0.05). Some of the main active components were significantly correlated with the bacteria, such as Enterococcus, Lactococcus and Carnobacterium.

Conclusion: Not only Eurotium, but also the bacteria were involved in the changes of metabolomics profile in fermented FBT. The present study assists in providing new insights into metabolomics profile generation in fermented tea. The present research lays a foundation for controlling the FBT fermentation by artificial inoculation to improve quality. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11365DOI Listing
June 2021

First Report of broad bean wilt virus-2 in marigold (Tagetes erecta L.) in China.

Plant Dis 2021 Jun 3. Epub 2021 Jun 3.

College of Bioscience and Resource Environment, Beijing University of Agriculture, Plant Pathology, Beijing, China;

Broad bean wilt virus 2 (BBWV-2), a member of the genus Fabavirus in the family Secoviridae mainly transmitted by aphids, has been recognized as a severe pathogen affecting the production of horticultural and ornamental plants worldwide (Xia et al. 2020). The virus was reported to infect many plant species mostly belonging to the family Fabaceae in China (Wang et al. 2017). In August 2018, marigold plants with the symptom of mosaic were observed in the field of Huairou, Beijing (Figure S1). Total RNA was extracted from symptomatic leaf samples from a single plant with TRIzol reagent (Invitrogen, Carlsbad, CA) with a standard procedure following the manufacturer's instructions, and small RNAs were isolated for deep sequencing library construction with Illumina TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA). The high-throughput sequencing was carried out on an Illumina HiSeq2500 platform. After raw data process, 8,911,917 clean reads were gained and further de novo assembled into contigs with CLC Genomics Workbench software. BLASTN and BLASTX analysis against the GenBank database showed that 81 of the 9,495 assembled contigs shared high nucleotide (nt) sequence identity with the bipartite genome of BBWV-2 isolate Gyp (KX686589-KX686590 for RNA1 and RNA2, 89% of the genome coverage and 90% nt identity) and 34 with high nt sequence identity of cucumber mosaic virus(CMV) from Tagetes erecta (EU665000-EU665002 for RNA1-RNA3,89% of the genome coverage and 96% nt sequence identity) with sequence coverage ranging from 24-fold to 8,078-fold at different genome positions. To further confirm the presence of BBWV-2, a RNA2 specific primer pair targeting the coat protein region (F1423-1448,5-CTGACAGAGGAATACTATTTCCAAAG-3;R2692-2719,5-CCTGTAAAATTGATATCTCCGGACAAAC-3) was designed from the obtained HTS sequence and reverse transcription-polymerase chain reaction (RT-PCR) was conducted. The 1.3 kb amplicon was ligated to pMD19-T vector (TaKaRa, Dalian, China) and sequenced. Sequence analysis showed it (BBWV-2-marigold, MW322809) shared 99% nt sequence identity with the Gyp isolate infecting Gynura procumbens from South Korea (LC497425.1). Phylogenetic analysis constructed with MEGA6 with the CP nt sequence of other reported BBWV-2 isolates showed BBWV-2-marigold clustered closely with the isolates from South Korea infecting Gynura procumbens (Figure S2), in accordance with the sequence identity analysis. Further RT-PCR with primer pair targeting the RNA1 (F3025-3050 5-GACAGAGTGATATTCCTAATCGAGAT-3; R4035-4062CACTCAATGC AATAAAGGTCTGGCACCT) was conducted and specific bands with the expected size of 1.0 kb were obtained in the agarose gel (data not shown), which further confirmed the existence of BBWV-2.A total of 16 marigold leaf samples(7 from Huairou and 9 from Yanqing) with mosaic symptom were collected and tested by RT-PCR with the abovementioned primer pair, and 4 from Huairou were BBWV-2 positive. Sequence analysis showed that these 4 isolates shared 100% nt sequence identity with the former sequenced isolateBBWV-2-marigold. Furthermore, CMV specific primer pair targeting the CP (F: 5-ATGGACAAATCTGGATCTCCCAAT-3/R: 5-CTAAGTCGGG AGCATCCGTGAGAT-3) were designed to detect the existence of CMV in these samples and results showed that all these 16 samples were positive for CMV. To the best of our knowledge, this is the first report of BBWV-2 in marigold in China.These findings will assist investigations on the epidemiology of diseases caused by BBWV2 in China.
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http://dx.doi.org/10.1094/PDIS-01-21-0078-PDNDOI Listing
June 2021

The biological role of MutT in the pathogenesis of the zoonotic pathogen serotype 2.

Virulence 2021 12;12(1):1538-1549

MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Lab of Animal Bacteriology, Ministry of Agriculture, OIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

() is an important rising pathogen that causes serious diseases in humans and pigs. Although some putative virulence factors of have been identified, its pathogenic mechanisms are largely unclear. Here, we identified a putative virulence-associated factor MutT, which is unique to serotype 2 (SS2) virulent strains. To investigate the biological roles of MutT in the SS2 virulent strain ZY05719, the knockout mutant (Δ) was generated and used to explore the phenotypic and virulent variations between the parental and Δ strains. We found that the mutation significantly inhibited cell growth ability, shortened the chain length, and displayed a high susceptibility to HO-induced oxidative stress. Moreover, this study revealed that MutT induced the adhesion and invasion of SS2 to host cells. Deletion of increased microbial clearance in host tissues of the infected mice. Sequence alignment results suggested that was encoded in a strain-specific manner, in which the detection was strongly linked to bacterial pathogenicity. In both zebrafish and mice infection models, the virulence of Δ was largely reduced compared with that of ZY05719. Overall, this study provides compelling evidence that MutT is indispensable for the virulence of SS2 and highlights the biological role of MutT in bacteria pathogenesis during infection.
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http://dx.doi.org/10.1080/21505594.2021.1936770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183525PMC
December 2021

Facies-Controlled Geostatistical Porosity Model for Estimation of the Groundwater Potential Area in Hongliu Coalmine, Ordos Basin, China.

ACS Omega 2021 Apr 8;6(15):10013-10029. Epub 2021 Apr 8.

Tianjin Branch of CNOOC Limited, Tianjin 300452, China.

Accurate and reliable evaluations of potential groundwater areas are of significance in the hydrogeological assessments of coalfields because water inrush disasters may be caused by unclear groundwater potential. A three-dimensional geological model of porosity based on deterministic modeling and a facies-controlled method are used to determine the groundwater potential of the coal measure aquifer. The modeling processes are as follows: based on the interlayer and discontinuity (faults) data extracted from boreholes and geological maps, an integrated sequence framework model is developed. Using the results of sedimentary microfacies identification and the method of deterministic modeling, a sedimentary microfacies model is successfully established. Finally, based on facies-controlled and sequential Gaussian methods, an effective porosity model is established that can predict the groundwater potential. The predicted results show that sandstones sedimented in channel, point bar, and batture environments possess high effective porosity and strong groundwater potential; however, the sandstones sedimented in interdistributary bays, flood plains, and sand sheets possess low effective porosity. Model validation was performed based on the hydrological pumping test data collected from observation boreholes, drainage water inflow data from dewatered boreholes in the tunnel around workface, and the mine water inflow in tunnels and the workfaces. The validation analysis results show that the effective porosity and sedimentary facies were correlated with the actual flux. The predicted results are consistent with the actual flux data, validating the predicted model.
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http://dx.doi.org/10.1021/acsomega.0c06166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153666PMC
April 2021

Gastrointestinal lipolysis and trans-epithelial transport of SMEDDS oral route.

Acta Pharm Sin B 2021 Apr 10;11(4):1010-1020. Epub 2021 Mar 10.

Key Laboratory of Smart Drug Delivery of MOE, School of Pharmacy, Fudan University, Shanghai 201203, China.

Self-microemulsifying drug delivery systems (SMEDDSs) have recently returned to the limelight of academia and industry due to their enormous potential in oral delivery of biomacromolecules. However, information on gastrointestinal lipolysis and trans-epithelial transport of SMEDDS is rare. Aggregation-caused quenching (ACQ) fluorescent probes are utilized to visualize the behaviors of SMEDDSs, because the released probes during lipolysis are quenched upon contacting water. Two SMEDDSs composed of medium chain triglyceride and different ratios of Tween-80 and PEG-400 are set as models, meanwhile Neoral® was used as a control. The SMEDDS droplets reside in the digestive tract for as long as 24 h and obey first order kinetic law of lipolysis. The increased chain length of the triglyceride decreases the lipolysis of the SMEDDSs. imaging of main tissues and histological examination confirm the trans-epithelial transportation of the SMEDDS droplets. Approximately 2%-4% of the given SMEDDSs are transported the lymph route following epithelial uptake, while liver is the main termination. Caco-2 cell lines confirm the cellular uptake and trans-epithelial transport. In conclusion, a fraction of SMEDDSs can survive the lipolysis in the gastrointestinal tract, permeate across the epithelia, translocate the lymph, and accumulate mainly in the liver.
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http://dx.doi.org/10.1016/j.apsb.2021.03.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105768PMC
April 2021

Dual Functional Pd-Catalyzed Multicomponent Reaction by Umpolung Chemistry of the Oxygen Atom in Electrophiles.

J Org Chem 2021 05 12;86(9):6847-6854. Epub 2021 Apr 12.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, 3663 North Zhongshan Road, Shanghai, China 200062.

A Pd-catalyzed multicomponent reaction was developed by trapping oxomium ylide with nitrosobenzene via Pd-promoted umpolung chemistry. The Pd catalyst plays two important roles: diazo compound decomposed catalyst and Lewis acid for the activation of nitrosobenzene. This strategy provides some insight into a new way for discovery of multicomponent methodology to construct complex molecules. The developed method also provides rapid access to a series of -(2-oxy) hydroxylamine derivatives, which exhibit good anticancer activity in osteosarcoma cells.
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http://dx.doi.org/10.1021/acs.joc.0c02413DOI Listing
May 2021

Effects of Qingguang'an containing serum on the expression levels of autophagy-related genes in human Tenon's fibroblasts induced by transforming growth factor beta 1.

J Tradit Chin Med 2021 04;41(2):236-245

Ophthalmology Department, the First Affiliated Hospital, Hunan University of TCM, Changsha 410007, China.

Objective: To explore the effects of Qingguang'an () containing serum on the expression levels of autophagy related genes in the transforming growth factor beta 1 (TGF-β1)-activated human Tenon's fibroblasts (HTFs).

Methods: (a) Primary HTFs were stimulated by TGF-β1 and underwent immunohistochemistry, which established a cell model after Glaucoma filtration surgery (GFS). (b) The cell models were divided into 4 group: normal group (normal cells), model group (+TGF-β1),treatment group (+TGF-β1+ medicated serum), and positive control group (TGF-β1+ rapamycin). Then, Qingguang'an medicated serum with optimum concentration was added to the corresponding group. The autophagy positive cells were identified by the Cyto-ID autophagy detection kits under fluorescent microscope and Cytation 5 multifunctional instrument for cell imaging. And the mean fluorescence intensity of autophagy positive cells was determined by flow cytometry. The expression levels of autophagy related genes - Beclin-1, autophagy related gene 5 (ATG-5), and microtubule-associated protein 1 light chain 3 (LC-3Ⅱ were detected by quantitative reverse transcription-polymerase chain reaction and Western blot analysis.

Results: Compared with the normal group and the model group, the relative mRNA expression levels of autophagy-related genes (Beclin-1, ATG-5 and LC-3Ⅱ in the experimental group were notably increased (P < 0.05, P < 0.01), and with the extension of treatment time, it had an increasing trend (48 h was more obvious), which showed a certain time dependency; the protein expression levels of autophagy-related genes (Beclin-1, ATG-5, and LC-3Ⅱ were significantly increased in the experimental group (P < 0.05, P < 0.01). With the prolongation of treatment time, there was an increasing trend (48 h was relatively obvious), and it revealed a certain time dependency.

Conclusion: The Qingguang'an medicated serum could up-regulate autophagy related genes (Beclin1, ATG5, and LC3Ⅱ in the TGF-β1-activated HTFs.
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April 2021

Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system.

J Microbiol 2021 May 29;59(5):481-490. Epub 2021 Mar 29.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named "Fuzi", which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in short-term continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.
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http://dx.doi.org/10.1007/s12275-021-0515-zDOI Listing
May 2021

Seven-year observation of posterior corneal elevation after small incision lenticule extraction (SMILE) in patients with moderate and high myopia.

J Cataract Refract Surg 2021 Mar 9. Epub 2021 Mar 9.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Purpose: To investigate the long-term changes in posterior corneal elevation in moderate and high myopia after small incision lenticule extraction (SMILE).

Setting: Eye & ENT Hospital, Fudan University.

Design: Prospective case series.

Methods: Thirty-three eyes of 20 patients (30.1+/-9.5 years, 7 male and 13 female, spherical equivalent [SE] range -4.00 to -8.75 D, average SE -6.25+/-1.29 D) who underwent SMILE were included. Changes in the posterior corneal elevation at central points (PCE), the thinnest point (PTE), the maximal point (PME), and an additional 20 points within the 4-mm area of the best-fit sphere were evaluated with a Pentacam (Oculus Optikgerate GmbH, Wetzlar, Germany) during a 7-year follow-up period. Mixed linear models were used to evaluate changes with P-values <0.05. Correlations of elevation changes and residual bed thickness (RBT) were also evaluated.

Results: No ectasia was observed among the 33 eyes. The safety index was 1.08, and the efficacy index was 1.03. The mean change in PCE at 1, 3, 5, and 7 years was -1.42+/-0.95, -2.67+/-0.98, -2.44+/-1.06, and -1.91+/-0.92 [micro]m, respectively. Significant differences were found at 3, 5, and 7 years (P=0.007, 0.023, and 0.040, respectively). PTE was significantly reduced at each time point compared to baseline. The mean change was -2.82+/-1.19, -3.55+/-1.22, -3.47+/-1.32, and -2.39+/-1.15 [micro]m, respectively (P=0.019, 0.004, 0.010, and 0.039, respectively). PME changed 2.45+/-1.02 [micro]m at 7 years compared to baseline (P=0.017). The changes in PCE and PME negatively correlated with the RBT.

Conclusions: Long-term posterior corneal elevation was stable in moderate and high myopia after SMILE.
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http://dx.doi.org/10.1097/j.jcrs.0000000000000639DOI Listing
March 2021

Stabilizing black-phase formamidinium perovskite formation at room temperature and high humidity.

Science 2021 03;371(6536):1359-1364

Key Laboratory of Flexible Electronics (KLoFE) and Institute of Advanced Materials (IAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, Jiangsu, China.

The stabilization of black-phase formamidinium lead iodide (α-FAPbI) perovskite under various environmental conditions is considered necessary for solar cells. However, challenges remain regarding the temperature sensitivity of α-FAPbI and the requirements for strict humidity control in its processing. Here we report the synthesis of stable α-FAPbI, regardless of humidity and temperature, based on a vertically aligned lead iodide thin film grown from an ionic liquid, methylamine formate. The vertically grown structure has numerous nanometer-scale ion channels that facilitate the permeation of formamidinium iodide into the lead iodide thin films for fast and robust transformation to α-FAPbI A solar cell with a power-conversion efficiency of 24.1% was achieved. The unencapsulated cells retain 80 and 90% of their initial efficiencies for 500 hours at 85°C and continuous light stress, respectively.
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http://dx.doi.org/10.1126/science.abf7652DOI Listing
March 2021

MicroRNA‑378d inhibits by targeting in vitamin D deficient ovarian granulosa cells.

Mol Med Rep 2021 05 24;23(5). Epub 2021 Mar 24.

Department of Reproductive Center, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

Vitamin D (VD) is not only associated with bone growth and development, but is also closely associated with numerous other pathological conditions. The present study aimed to investigate the effect of microRNA (miRNA/miR)‑378d on ovarian granulosa cells by regulating the round spermatid basic protein 1 () in the absence of VD. The abnormal expression of miRNAs in ovarian tissues of the VD‑deficient mouse was analyzed using transcriptome sequencing. miR‑378d, glucose transporter 4 () and aromatase () expression levels were examined via reverse transcription‑quantitative (RT‑q)PCR and western blotting. The expression levels of , and were detected in transfected mouse ovarian granulosa cells. The targeting regulation between miR‑378d and was verified using double reporter gene assay and functional rescue experiments. Among the 672 miRNAs that were differentially expressed, cluster analysis revealed that 17 were significantly upregulated and 16 were significantly downregulated. Moreover, miR‑378d showed significant upregulation, which was further verified via RT‑qPCR. It was identified that the protein expression level of was significantly downregulated. Furthermore, mRNA expression was significantly decreased in the mimic group but markedly increased in the inhibitor group. By contrast, the mRNA expression levels of and did not demonstrate any significant difference. The western blotting results indicated that the protein expression levels of and were decreased and increased, respectively, while did not show any significant change. In addition, the double reporter gene experiments confirmed that was the target gene of miR‑378d. Collectively, the present results demonstrated that miR‑378d was abnormally overexpressed in the ovarian tissues of the VD‑deficient mice, and that miR‑378d could inhibit production by targeting , which may lead to insulin resistance.
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http://dx.doi.org/10.3892/mmr.2021.12008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985995PMC
May 2021

Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain.

Sci Adv 2021 Mar 17;7(12). Epub 2021 Mar 17.

School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Multiphoton fluorescence microscopy is a powerful technique for deep-tissue observation of living cells. In particular, three-photon microscopy is highly beneficial for deep-tissue imaging because of the long excitation wavelength and the high nonlinear confinement in living tissues. Because of the large spectral separation of fluorophores of different color, multicolor three-photon imaging typically requires multiple excitation wavelengths. Here, we report a new three-photon excitation scheme: excitation to a higher-energy electronic excited state instead of the conventional excitation to the lowest-energy excited state, enabling multicolor three-photon fluorescence imaging with deep-tissue penetration in the living mouse brain using single-wavelength excitation. We further demonstrate that our excitation method results in ≥10-fold signal enhancement for some of the common red fluorescent molecules. The multicolor imaging capability and the possibility of enhanced three-photon excitation cross section will open new opportunities for life science applications of three-photon microscopy.
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http://dx.doi.org/10.1126/sciadv.abf3531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968831PMC
March 2021

Low FOXJ2 expression is associated with unfavorable postoperative prognosis of patients with epithelial ovarian cancer.

Medicine (Baltimore) 2021 Mar;100(10):e24759

Department of Gynecology and Obstetrics, Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Abstract: The forkhead box (FOX) family is a large and diverse group of transcription factors. Forkhead box J2 (FOXJ2) is a member of the FOX family that is aberrantly expressed in a variety of cancers. However, its role in epithelial ovarian cancer (EOC) remains elusive. The purpose of this study was to evaluate the prognostic value of FOXJ2 expression in patients with epithelial ovarian cancer.The current study retrospectively included 151 patients with EOC from January 2013 to September 2016. FOXJ2 expression was analyzed by immunohistochemistry based on tissue microarrays. Then, the prognostic value of FOXJ2 expression and clinical outcomes were evaluated by Kaplan-Meier and cox regression analysis.Low FOXJ2 expression was associated with high International Federation of Gynecology and Obstetrics (FIGO) stage. Kaplan-Meier curves showed that high FOXJ2 expression was associated with improved median overall survival (OS, 57.9 vs 31.9 months; P = .037) and longer median progression-free survival (PFS, 31.8 vs 18.1 months; P = .012). Univariate analysis demonstrated that FOXJ2 expression was significantly correlated with OS and PFS in patients with epithelial ovarian cancer. Multivariate analysis revealed FOXJ2 expression as an independent prognostic factor of progression-free survival of epithelial ovarian cancer patients.Low FOXJ2 expression is a novel adverse prognostic factor of clinical outcome in epithelial ovarian cancer.
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http://dx.doi.org/10.1097/MD.0000000000024759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969229PMC
March 2021

Coarse-Grained Simulation of Mechanical Properties of Single Microtubules With Micrometer Length.

Front Mol Biosci 2020 15;7:632122. Epub 2021 Feb 15.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.

Microtubules are one of the most important components in the cytoskeleton and play a vital role in maintaining the shape and function of cells. Because single microtubules are some micrometers long, it is difficult to simulate such a large system using an all-atom model. In this work, we use the newly developed convolutional and K-means coarse-graining (CK-CG) method to establish an ultra-coarse-grained (UCG) model of a single microtubule, on the basis of the low electron microscopy density data of microtubules. We discuss the rationale of the micro-coarse-grained microtubule models of different resolutions and explore microtubule models up to 12-micron length. We use the devised microtubule model to quantify mechanical properties of microtubules of different lengths. Our model allows mesoscopic simulations of micrometer-level biomaterials and can be further used to study important biological processes related to microtubule function.
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http://dx.doi.org/10.3389/fmolb.2020.632122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917235PMC
February 2021

Iron speciation and annual records in black coral as new proxy for mining and environmental impacts.

Sci Total Environ 2021 Jul 19;776:145965. Epub 2021 Feb 19.

State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China.

Iron (Fe) is a micronutrient and plays an important role in regulating ocean primary production and consequently changing oceanic CO uptake. However, approaches for high-resolution of Fe records in marine environment has been a great challenge. In this study, we report for the first time an annual Fe record on black coral organic skeleton from the northern South China Sea (SCS) as an archive to study the environmental change during the past century. In situ micro-Raman, synchrotron micro X-ray absorption near edge spectroscopy (μ-XANES) and synchrotron micro X-ray fluorescence (μ-XRF) were applied to investigate the Fe speciation and the radial Fe profile in black coral. The preliminary results from micro-Raman and synchrotron micro XANES analysis demonstrated that Fe in black coral was mainly combined with 3,4-dihydroxyphenylalanine (dopa) as tris-DOPA-Fe complex. Such spatial coordination structure of complexation makes Fe have high affinity with dopa in black coral. Furthermore, elevated Fe concentration in Fe profile recorded on synchrotron μ-XRF spectra with 2.5 μm resolution corresponded well to the exploitation history of the adjacent onshore Tiandu Iron Mine (Sanya, China) from 1939 to 1960. Other distinct Fe peak coincides with the war activities in 1970s. The findings presented in this work indicate that the high-resolution iron record with low annual growth rate (~17.8 μm year) of black coral may serve as a proxy of marine environmental record.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145965DOI Listing
July 2021

Curcumin promotes venous thrombi resolve process in a mouse deep venous thrombosis model via regulating miR-499.

Microvasc Res 2021 07 23;136:104148. Epub 2021 Feb 23.

Department of Cardiology, The Fourth People's Hospital of Shenyang, Shenyang, China. Electronic address:

Background/aims: The morbidity of deep venous thrombosis (DVT) is increasing rapidly and the current therapeutic strategies for DVT are unsatisfactory. Accumulating evidence suggest that venous thrombi resolve (VTR) may provide new insights into DVT therapeutic strategies. The aim of this study was to investigate the role of curcumin in VTR process and try to reveal the potential mechanism.

Methods: Immunofluorescence and HE staining were performed to investigate the therapeutic angiogenesis effect of curcumin in VTR process. Microarray analysis and RT-PCR were performed to examine the expression level of miR-499 in thrombosis after curcumin administration. Cell proliferation, migration and angiogenesis capacity were tested by CCK8 assay, Transwell assay and Tube formation assay, respectively. Dual-luciferase reporter assay (DLR) was used to confirm the connection between miR-499 and paired phosphate and tension homology deleted on chromosome ten (PTEN).

Results: We found that curcumin could effectively promote VTR process by activating angiogenesis in thrombus in vivo. The expression of miR-499 exhibited notably downregulated after curcumin administration. The proangiogenic effect of curcumin in HUVECs could be blocked by miR-499 overexpression. In addition, we confirmed that miR-499 directly target to the 3'UTR region of PTEN.

Conclusion: Curcumin promotes VTR process in DVT through activating therapeutic angiogenesis. Mechanically, curcumin promotes therapeutic angiogenesis by regulating miR-499 mediated PTEN/VEGF/Ang-1 signaling pathway.
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http://dx.doi.org/10.1016/j.mvr.2021.104148DOI Listing
July 2021
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