Publications by authors named "Fei Tong"

95 Publications

Control the Neural Stem Cell Fate with Biohybrid Piezoelectrical Magnetite Micromotors.

Nano Lett 2021 Apr 13;21(8):3518-3526. Epub 2021 Apr 13.

School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.

Inducing neural stem cells to differentiate and replace degenerated functional neurons represents the most promising approach for neural degenerative diseases including Parkinson's disease, Alzheimer's disease, etc. While diverse strategies have been proposed in recent years, most of these are hindered due to uncontrollable cell fate and device invasiveness. Here, we report a minimally invasive micromotor platform with biodegradable helical () as the framework and superparamagnetic FeO nanoparticles/piezoelectric BaTiO nanoparticles as the built-in function units. With a low-strength rotational magnetic field, this integrated micromotor system can perform precise navigation in biofluid and achieve single-neural stem cell targeting. Remarkably, by tuning ultrasound intensity, thus the local electrical output by the motor, directed differentiation of the neural stem cell into astrocytes, functional neurons (dopamine neurons, cholinergic neurons), and oligodendrocytes, can be achieved. This micromotor platform can serve as a highly controllable wireless tool for bioelectronics and neuronal regenerative therapy.
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http://dx.doi.org/10.1021/acs.nanolett.1c00290DOI Listing
April 2021

Successful treatment of cardiac arrest following hysteroscopic surgery using extracorporeal membrane oxygenation: A case report.

Medicine (Baltimore) 2021 Apr;100(15):e25519

Intensive Care Unit, The First Affiliated Hospital of USTC, University of Science and Technology of China, Anhui, China.

Rationale: Cardiac arrest caused by water intoxication syndrome following hysteroscopic surgery is a rare but life-threatening occurrence. Extracorporeal membrane oxygenation (ECMO) is rarely used to treat water intoxication syndrome in hysteroscopic surgery. Here, we successfully treated a patient with water intoxication syndrome following hysteroscopic surgery with ECMO.

Patient Concerns: We report a rare case of cardiac arrest during hysteroscopic surgery treated with veno-venous (VV) ECMO.

Diagnosis: Water poisoning syndrome was diagnosed by electrolyte examination, the lowest value of serum sodium was 110.7 mmol/L.

Interventions: VV-ECMO was prescribed as a measure after traditional cardiopulmonary resuscitation.

Results: ECMO was successfully evacuated on day 5 and the patient was discharged on day 45.

Conclusion: Mastering the hysteroscopic operative techniques and using a bipolar hysteroscopic generator, isotonic fluid, perfusion pressures less than 100 mm Hg, and local anesthesia may reduce the risk of hysteroscopic water intoxication syndrome. During hysteroscopic surgery, patients may experience cardiac arrest and fatal water intoxication syndrome. Even when traditional cardiopulmonary resuscitation is successful, VV ECMO may contribute to the recovery of brain function if oxygenation is not maintained.
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http://dx.doi.org/10.1097/MD.0000000000025519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052025PMC
April 2021

Effects of Cognitive Behavioral Therapy for Depression and Anxiety, Response Rates and Adverse Events in Patients with Locoregional Advanced Nasopharyngeal Carcinoma.

Integr Cancer Ther 2021 Jan-Dec;20:15347354211006179

Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.

Purpose: This retrospective study investigated the effects of cognitive behavioral therapy (CBT) on depression, anxiety, response rates, and adverse events in patients with locoregional advanced nasopharyngeal carcinoma (NPC).

Methods: A total of 269 patients with diagnosis of stage III-IVA NPC received either CBT plus chemoradiotherapy (CBT group, n = 136) or treatment as usual (TAU) plus chemoradiotherapy (TAU group, n = 133). Patients in the CBT group received a series of 6 CBT sessions for 6 weeks during concurrent chemoradiotherapy. Depression and anxiety were assessed using the Hospital Anxiety and Depression Scale (HADS) score at baseline, the completion of radiotherapy, and 6, 12, and 24 months after radiotherapy. Response rates and adverse events were also evaluated.

Results: Patients in the CBT group showed significantly less depression and anxiety than patients in the TAU group after the completion of radiotherapy ( < .05). Complete response rates were 99.3% (135/136) and 92.5% (123/133) in the CBT group and TAU group with a small effect size (Phi coefficient = .171), respectively ( = .005). Compared with the TAU group, the CBT group showed a significantly lower incidence of acute adverse events and late toxic effects.

Conclusions: The addition of CBT to chemoradiotherapy significantly reduced depressive and anxiety symptoms. CBT combined with chemoradiotherapy is associated with improved response rates, with reduced incidence of toxic effects in patients with locoregional advanced NPC. Based on this study, we registered a randomized controlled clinical trials to better define the role of CBT in patients with locoregional advanced NPC (Registration number: ChiCTR2000034701).
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http://dx.doi.org/10.1177/15347354211006179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020041PMC
March 2021

Novel Functionalized Cannabinoid Receptor Probes: Development of Exceptionally Potent Agonists.

J Med Chem 2021 Apr 24;64(7):3870-3884. Epub 2021 Mar 24.

Department of Molecular Medicine, The Scripps Research Institute, Jupiter, Florida 33458, United States.

We report the development of novel cannabinergic probes that can stabilize the cannabinoid receptors (CBRs) through tight binding interactions. Ligand design involves the introduction of select groups at a judiciously chosen position within the classical hexahydrocannabinol template (monofunctionalized probes). Such groups include the electrophilic isothiocyanato, the photoactivatable azido, and the polar cyano moieties. These groups can also be combined to produce bifunctionalized probes potentially capable of interacting at two distinct sites within the CBR-binding domains. These novel compounds display remarkably high binding affinities for CBRs and are exceptionally potent agonists. A key ligand (, AM11245) exhibits exceptionally high potency in both and assays and was designated as "megagonist," a property attributed to its tight binding profile. By acting both centrally and peripherally, distinguishes itself from our previously reported "megagonist" AM841, whose functions are restricted to the periphery.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02053DOI Listing
April 2021

Oxa-adamantyl cannabinoids.

Bioorg Med Chem Lett 2021 Apr 24;38:127882. Epub 2021 Feb 24.

Center for Drug Discovery, Department of Chemistry and Chemical Biology, and Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, United States. Electronic address:

As a continuation of earlier work on classical cannabinoids bearing bulky side chains we report here the design, synthesis, and biological evaluation of 3'-functionalized oxa-adamantyl cannabinoids as a novel class of cannabinergic ligands. Key synthetic steps involve nucleophilic addition/transannular cyclization of aryllithium to epoxyketone in the presence of cerium chloride and stereoselective construction of the tricyclic cannabinoid nucleus. The synthesis of the oxa-adamantyl cannabinoids is convenient, and amenable to scale up allowing the preparation of these analogs in sufficient quantities for detailed in vitro evaluation. The novel oxa-adamantyl cannabinoids reported here were found to be high affinity ligands for the CB1 and CB2 cannabinoid receptors. In the cyclase assay these compounds were found to behave as potent and efficacious CB1 receptor agonists. Isothiocyanate analog AM10504 is capable of irreversibly labeling both the CB1 and CB2 receptors.
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http://dx.doi.org/10.1016/j.bmcl.2021.127882DOI Listing
April 2021

Magnesium-Based Micromotors as Hydrogen Generators for Precise Rheumatoid Arthritis Therapy.

Nano Lett 2021 03 24;21(5):1982-1991. Epub 2021 Feb 24.

Department of Medicine Ultrasonics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Hydrogen therapy is an emerging and highly promising strategy for the treatment of inflammation-related diseases. However, nonpolarity and low solubility of hydrogen under the physiological conditions results in a limited therapeutic effect. Herein, we develop a biocompatible magnesium micromotor coated with hyaluronic acid as a hydrogen generator for precise rheumatoid arthritis management. The hydrogen bubbles generated locally not only function as a propellant for the motion but also function as the active ingredient for reactive oxygen species (ROS) and inflammation scavenging. Under ultrasound guidance, the micromotors are injected intra-articularly, and the dynamics of the micromotors can be visualized. By scavenging ROS and inflammation via active hydrogen, the oxidative stress is relieved and the levels of inflammation cytokines are reduced by our micromotors, showing prominent therapeutic efficacy in ameliorating joint damage and suppressing the overall arthritis severity toward a collagen-induced arthritis rat model. Therefore, our micromotors show great potential for the therapy of rheumatoid arthritis and further clinical transformation.
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http://dx.doi.org/10.1021/acs.nanolett.0c04438DOI Listing
March 2021

Dexmedetomidine attenuates lipopolysaccharide-induced acute liver injury in rats by inhibiting caveolin-1 downstream signaling pathway.

Biosci Rep 2021 Mar;41(3)

Department of Anesthesiology, Huzhou Maternal and Child Health Care Hospital, Huzhou, Zhejiang Province, China.

Objective: The aim of the present study is to investigate the anti-injury and anti-inflammatory effects of dexmedetomidine (Dex) in acute liver injury induced by lipopolysaccharide (LPS) in Sprague-Dawley rats and its possible mechanism.

Methods: The acute liver injury model of male rats was established by injecting LPS into tail vein. The mean arterial pressure (MAP) of rats was recorded at 0-7 h, and lactic acid was detected at different time points. Wet/dry weight ratio (W/D) was calculated. Pathological changes of rat liver were observed by HE staining. ALT and AST levels in serum were detected. The activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) in liver tissue homogenate and the levels of IL-1β and IL-18 in serum were detected by ELISA. Protein levels of Caveolin-1 (Cav-1), TLR-4 and NLRP3 in liver tissue were tested by immunohistochemistry method. The expression of Cav-1, TLR-4 and NLRP3 mRNA in liver tissue was detected by quantitative polymerase chain reaction (qPCR) to explore its related mechanism.

Results: Compared with NS group, serum lactic acid, W/D of liver tissue, MPO, SOD, IL-1β and IL-18 were significantly increased and MAP decreased significantly in LPS group and D+L group. However, compared with NS group, D group showed no significant difference in various indicators. Compared with LPS group, MPO, SOD, IL-1β and IL-18 were significantly decreased and MAP was significantly increased in D+L group. D+L group could significantly increase the level of Cav-1 protein and decrease the level of TLR-4 and NLRP3 protein in liver tissue caused by sepsis. The expression of Cav-1 mRNA was significantly up-regulated and the expression of TLR-4 and NLRP3 mRNA was inhibited in D+L group.

Conclusion: Dex pretreatment protects against LPS-induced actue liver injury via inhibiting the activation of the NLRP3 signaling pathway by up-regulating the expression of Cav-1 by sepsis.
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http://dx.doi.org/10.1042/BSR20204279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938455PMC
March 2021

The effect of organic solvent washing on the structure of hydrochar-based dissolved organic matters and its potential environmental toxicity.

Environ Sci Pollut Res Int 2021 Jan 23. Epub 2021 Jan 23.

Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, China.

With the increased interest in the practical use of hydrochar, concerns about the possible environmental biotoxicity of hydrochar and its released dissolved organic matters (DOM) have grown. As a common method for removing bio-oil on the surface of hydrochar, the effect of organic solvent washing on the properties of hydrochar released DOM remains unclear. In this study, we made a comprehensive comparison of hydrochar properties and molecule structure as well as biotoxicity of DOM released from HC (raw hydrochar) and THC (hydrochar washed by tetrahydrofuran). The results indicated that the mass loss of hydrochar was obvious after tetrahydrofuran (THF) washing, and a decline of H/C atomic ratio and increase of N/C and O/C atomic ratios was observed based on Van Krevelen (VK) diagram. This result was further confirmed by FTIR, C NMR, and XPS results. Meanwhile, the molecule structure of DOM was shifted to lower molecule weight with higher O-contain compounds after THF extraction due to the demethanation process. However, the biotoxicity experiments indicated that both extracted DOM had no significant impact on germination rate of wheat, and HC-treated sample even exhibited growth superiority. Nevertheless, potential toxicity was observed with the increase of the activity of antioxidant enzymes, and THF washing aggravated the potential oxidative damage through increasing the aromaticity of DOM. Such understanding highlights the importance of evaluating hydrochar and its released DOM before applications, so as to reduce the potential environment biotoxicity.
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http://dx.doi.org/10.1007/s11356-021-12517-5DOI Listing
January 2021

Light-Powered Autonomous Flagella-Like Motion of Molecular Crystal Microwires.

Angew Chem Int Ed Engl 2021 02 23;60(5):2414-2423. Epub 2020 Dec 23.

Department of Chemistry, University of California, Riverside, 501 Big Springs Road, Riverside, CA, 92521, USA.

The ability to exhibit life-like oscillatory motion fueled by light represents a new capability for stimuli-responsive materials. Although this capability has been demonstrated in soft materials like polymers, it has never been observed in molecular crystals, which are not generally regarded as dynamic objects. In this work, it is shown that molecular crystalline microwires composed of (Z)-2-(3-(anthracen-9-yl)allylidene)malononitrile ((Z)-DVAM) can be continuously actuated when exposed to a combination of ultraviolet and visible light. The photo-induced motion mimics the oscillatory behavior of biological flagella and enables propagation of microwires across a surface and through liquids, with translational speeds up to 7 μm s . This is the first example of molecular crystals that show complex oscillatory behavior under continuous irradiation. A model that relates the rotation of the transition dipole moment between reversible E→Z photoisomerization to the microscopic torque can qualitatively reproduce how the rotational frequency depends on light intensity and polarization.
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http://dx.doi.org/10.1002/anie.202012417DOI Listing
February 2021

X-ray2Shape: Reconstruction of 3D Liver Shape from a Single 2D Projection Image.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:1608-1611

Computed tomography (CT) and magnetic resonance imaging (MRI) scanners measure three-dimensional (3D) images of patients. However, only low-dimensional local two-dimensional (2D) images may be obtained during surgery or radiotherapy. Although computer vision techniques have shown that 3D shapes can be estimated from multiple 2D images, shape reconstruction from a single 2D image such as an endoscopic image or an X-ray image remains a challenge. In this study, we propose X-ray2Shape, which permits a deep learning-based 3D organ mesh to be reconstructed from a single 2D projection image. The method learns the mesh deformation from a mean template and deep features computed from the individual projection images. Experiments with organ meshes and digitally reconstructed radiograph (DRR) images of abdominal regions were performed to confirm the estimation performance of the methods.
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http://dx.doi.org/10.1109/EMBC44109.2020.9176655DOI Listing
July 2020

RDCPF: A Redundancy-Based Duty-Cycling Pipelined-Forwarding MAC for Linear Sensor Networks.

Sensors (Basel) 2020 Sep 30;20(19). Epub 2020 Sep 30.

School of Cyber Science and Engineering, Southeast University, Nanjing 210096, China.

Existing duty-cycling and pipelined-forwarding (DCPF) protocols applied in battery-powered wireless sensor networks can significantly alleviate the sleep latency issue and save the energy of networks. However, when a DCPF protocol applies to a linear sensor network (LSN), it lacks the ability to handle the bottleneck issue called the energy-hole problem, which is mainly manifested due to the excessive energy consumption of nodes near the sink node. Without overcoming this issue, the lifespan of the network could be greatly reduced. To that end, this paper proposes a method of deploying redundant nodes in LSN, and a corresponding enhanced DCPF protocol called redundancy-based DCPF (RDCPF) to support the new topology of LSN. In RDCPF, the distribution of energy consumption of the whole network becomes much more even. RDCPF also brings improvements to the network in terms of network survival time, packet delivery latency, and energy efficiency, which have been shown through the extensive simulations in comparison with existing DCPF protocols.
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http://dx.doi.org/10.3390/s20195608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583853PMC
September 2020

LncRNA SNHG17 promotes the progression of oral squamous cell carcinoma by modulating miR-375/PAX6 axis.

Cancer Biomark 2021 ;30(1):1-12

Department of Orthodontics, Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine, Nanchang, Jiangxi, China.

Background: The prognosis of patients with recurrent and/or metastatic oral squamous cell carcinoma (OSCC) remains poor, and its incidence is especially high in developing countries. Multiple long non-coding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumor suppressors. This study aimed to probe into the role of lncRNA small nucleolar RNA host gene 17 (SNHG17) on the progression of OSCC.

Methods: The expression level of SNHG17 in OSCC samples was tested using quantitative real-time polymerase chain reaction (qRT-PCR). Human OSCC cell lines CAL-27 and Tca8113 were used in in vitro studies. Cell counting kit-8 (CCK-8) and BrdU assays were used to assess the effect of SNHG17 on OSCC cell proliferation. Flow cytometry was used to study the effect of SNHG17 on OSCC cell apoptosis. Transwell assay was conducted to detect the effect of SNHG17 on migration and invasion. Moreover, luciferase reporter assay was employed to confirm targeting relationship between miR-375 and SNHG17. Additionally, Western blot was used to observe the regulatory function of SNHG17 on PAX6.

Results: SNHG17 expression in OSCC clinical samples was significantly increased and was correlated with unfavorable pathological indexes. Its overexpression remarkably accelerated proliferation and metastasis of OSCC cells, while reduced apoptosis. Accordingly, knockdown of SNHG17 suppressed the malignant phenotypes of OSCC cells. Overexpression of SNHG17 significantly reduced the expression of miR-375 by sponging it, but enhanced the expression of PAX6.

Conclusion: SNHG17 is a sponge of tumor suppressor miR-375 in OSCC, enhances the expression of PAX6 indirectly, and functions as an oncogenic lncRNA.
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http://dx.doi.org/10.3233/CBM-191070DOI Listing
January 2021

Molecular Crystal Microcapsules: Formation of Sealed Hollow Chambers via Surfactant-Mediated Growth.

Angew Chem Int Ed Engl 2020 Dec 13;59(51):23035-23039. Epub 2020 Oct 13.

Chemistry Department, University of California, Riverside, Riverside, CA, 92521, USA.

Hollow organic molecular cocrystals comprised of 9-methylanthracene-1,2,4,5-tetracyanobenzene (9MA-TCNB) and naphthalene-1,2,4,5-tetracyanobenzene (NAPH-TCNB) were fabricated using a surfactant-mediated co-reprecipitation method. The crystals exhibit a narrow size distribution that can be easily tuned by varying the concentration of surfactant and incubation temperature. The rectangular crystals possess symmetrical twinned cavities with an estimated storage volume on the order of 10  L. An aqueous dye solution can be incorporated into the cavities during crystal growth and stored inside for up to several hours, confirming the sealed nature of the hollow chambers. Our results demonstrate that it is possible to harness non-classical crystal growth to fabricate organic molecular crystals with novel topologies.
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http://dx.doi.org/10.1002/anie.202009906DOI Listing
December 2020

Mechanical Properties and Photomechanical Fatigue of Macro- and Nanodimensional Diarylethene Molecular Crystals.

Nano Lett 2020 09 26;20(9):6744-6749. Epub 2020 Aug 26.

Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, United States.

The diarylethene derivative, 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)perfluorocyclopentene, undergoes a reversible photoisomerization between its ring-open and ring-closed forms in the solid-state and has applications as a photomechanical material. Mechanical properties of macrocrystals, nanowire single crystals, and amorphous films as a function of multiple sequential UV and visible light exposures have been quantified using atomic force microscopy nanoindentation. The isomerization reaction has no effect on the elastic modulus of each solid. But going from the macro- to the nanowire crystal results in a remarkable over 3-fold decrease in the elastic modulus. The macrocrystal and amorphous solids are highly resistant to photomechanical fatigue, while nanowire crystals show clear evidence of photomechanical fatigue attributed to a transition from crystal to amorphous forms. This study provides first experimental evidence of size-dependent photomechanical fatigue in photoreactive molecular crystalline solids and suggests crystal morphology and size must be considered for future photomechanical applications.
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http://dx.doi.org/10.1021/acs.nanolett.0c02631DOI Listing
September 2020

A Data-Gathering, Dynamic Duty-Cycling MAC Protocol for Large-Scale Wireless Sensor Networks.

Sensors (Basel) 2020 Jul 22;20(15). Epub 2020 Jul 22.

Faculty of Information Technology, Macau University of Science and Technology, Taipa 999078, Macau, China.

This paper presents a Data-gathering, Dynamic Duty-cycling (D3) protocol for wireless sensor networks. With a proposed duty-cycling MAC of high energy efficiency in D3, a routing scheme is naturally embedded to reduce protocol overhead. A packet can be forwarded in a pipelined fashion by staggering the sleep-wakeup schedules between two communicating nodes, which can significantly reduce end-to-end delay to meet real-time transmission requirements. To construct and maintain schedules, a grade and schedule establishment mechanism with a lightweight schedule error correction scheme is designed. In addition, based on the intrinsic characteristics of the network, an adaptive schedule maintenance scheme is proposed to dynamically adjust the node duty cycle to the network traffic load. The results based on the extensive OPNET simulations show that D3 can largely improve packet delivery ratio, energy efficiency and throughput, and reduce packet delivery latency.
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http://dx.doi.org/10.3390/s20154071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435929PMC
July 2020

Bone-Targeting Prodrug Mesoporous Silica-Based Nanoreactor with Reactive Oxygen Species Burst for Enhanced Chemotherapy.

ACS Appl Mater Interfaces 2020 Aug 21;12(31):34630-34642. Epub 2020 Jul 21.

School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University, Guangzhou 510515, China.

Cancer remains a primary threat to human lives. Recently, amplification of tumor-associated reactive oxygen species (ROS) has been used as a boosting strategy to improve tumor therapy. Here, we report on a bone-targeting prodrug mesoporous silica-based nanoreactor for combined photodynamic therapy (PDT) and enhanced chemotherapy for osteosarcoma. Because of surface modification of a bone-targeting biphosphate moiety and the enhanced permeability and retention effect, the formed nanoreactor shows efficient accumulation in osteosarcoma and exhibits long-term retention in the tumor microenvironment. Upon laser irradiation, the loaded photosensitizer chlorin e6 (Ce6) produces in situ ROS, which not only works for PDT but also functions as a trigger for controlled release of doxorubicin (DOX) and doxycycline (DOXY) from the prodrugs based on a thioketal () linkage. The released DOXY further promotes ROS production, thus perpetuating subsequent DOX/DOXY release and ROS burst. The ROS amplification induces long-term high oxidative stress, which increases the sensitivity of the osteosarcoma to chemotherapy, therefore resulting in enhanced tumor cell inhibition and apoptosis. The as-developed nanoreactor with combined PDT and enhanced chemotherapy based on ROS amplification shows significant promise as a potential platform for cancer treatment.
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http://dx.doi.org/10.1021/acsami.0c08992DOI Listing
August 2020

[Value of neutrophil-to-lymphocyte ratio in the classification diagnosis of coronavirus disease 2019].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 May;32(5):554-558

Department of Intensive Care Medicine, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230036, Anhui, China. Corresponding author: Tao Xiaogen, Email:

Objective: To investigate the clinical significance of neutrophil-to-lymphocyte ratio (NLR) in classification of patients with coronavirus disease 2019 (COVID-19).

Methods: A retrospective analysis was performed on 72 patients with COVID-19 admitted to the critical ward of Cancer Center of Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology in Wuhan from February to March in 2020. The patients were divided into two groups: moderate type (non-severe group) and severe/critical type (severe group). The results of white blood cell count (WBC), neutrophil count (NEU), lymphocyte count (LYM), interleukin-6 (IL-6) and D-dimer were collected at the 2nd day after admission from the two groups, and the NLR was calculated. The diagnostic value of WBC, NEU, LYM, IL-6, D-dimer and NLR on COVID-19 classification was evaluated by the receiver operating characteristic (ROC) curve.

Results: A total of 72 COVID-19 patients were enrolled, among whom 52 were moderate, 17 were severe, and 3 were critical. The most common clinical manifestations of patients were fever (70.8%), cough (36.1%), chest tightness and breathlessness (37.5%), diarrhea (15.3%), fatigue (15.3%), vomiting and nausea (11.1%), occasionally accompanied by acute dyspnea (2.8%), and only one patient had no clinical symptom (1.4%). The levels of WBC, NEU, IL-6, D-dimer and NLR in the severe group were significantly higher than those in the non-severe group [WBC (×10/L): 7.81±3.65 vs. 5.34±1.69, NEU (×10/L): 5.83±3.13 vs. 3.24±1.53, IL-6 (ng/L): 133.63 (71.09, 249.61) vs. 28.05 (6.41, 101.24), D-dimer (mg/L): 0.86 (0.31, 2.56) vs. 0.33 (0.20, 0.71), NLR: 6.14±4.75 vs. 2.66±1.93, all P < 0.05], and the level of LYM was significantly lower than that in the non-severe group (×10/L: 1.09±0.56 vs. 1.49±0.74, P < 0.05). The results of ROC curve analysis showed that the areas under ROC curve (AUC) of WBC, NEU, LYM, IL-6, D-dimer and NLR for COVID-19 classification were 0.790 [95% confidence interval (95%CI) was 0.684-0.897), 0.869 (95%CI was 0.789-0.949), 0.719 (95%CI was 0.592-0.847), 0.790 (95%CI was 0.682-0.898), 0.676 (95%CI was 0.526-0.827), and 0.888 (95%CI was 0.814-0.963) respectively. The AUC of NLR was the highest, which was of high diagnostic value; when the optimum cut-off value of NLR was 3.00, the sensitivity was 100%, and the specificity was 73.1%.

Conclusions: NLR can be used as a biomarker to predict classification of COVID-19 patients independently, which can provide a theoretical basis for the classification management of COVID-19 patients.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200413-00506DOI Listing
May 2020

[Analysis of gastrointestinal symptoms in 80 patients with coronavirus disease 2019].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Apr;32(4):412-416

Department of Critical Care Medicine, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230001, Anhui, China.

Objective: To investigate the clinical characteristics of gastrointestinal symptoms in patients with coronavirus disease 2019 (COVID-19) during the whole disease process, and provide reference for etiological diagnosis and treatment.

Methods: The clinical data of patients with COVID-19 admitted in the Infectious Diseases Branch of the First Affiliated Hospital of University of Science and Technology of China from January 22nd, 2020 to March 8th, 2020 were analyzed retrospectively. According to whether there were gastrointestinal symptoms (poor appetite, nausea/vomiting and diarrhea), all patients were divided into gastrointestinal symptom group and asymptomatic group. The characteristics of gastrointestinal symptoms, such as poor appetite, nausea, vomiting and diarrhea were counted and analyzed, and the correlation between gastrointestinal symptoms and gender, age, basic diseases, disease severity, laboratory examination and drug treatment were analyzed.

Results: A total of 80 COVID-19 patients were involved, 43 cases (53.8%) presented with poor appetite, 17 cases (21.3%) had nausea and vomiting, and 33 cases (41.3%) had diarrhea. Among them, 5 cases, 1 case and 4 cases respectively preformed poor appetite, nausea/vomiting and diarrhea before admission, while the others experienced gastrointestinal symptoms within 48 hours after admission. Duration of poor appetite, nausea/vomiting and diarrhea (days) of all patients were 5.3±2.1, 2.2±1.0 and 1.4±0.9, respectively. The patients with poor appetite were older than those without symptoms (years old: 48.2±17.6 vs. 39.3±15.1), albumin (Alb) level and the lymphocytes ratio were lower than those in asymptomatic group [Alb (g/L): 39.8 (35.7, 45.1) vs. 46.1 (42.6, 49.4), lymphocytes ratio: 0.19 (0.09, 0.28) vs. 0.28 (0.17, 0.35)], while the neutrophil ratio, the levels of C-reactive protein (CRP), D-dimer, and lactate dehydrogenase (LDH) were higher than those in asymptomatic group [the neutrophil ratio: 0.74 (0.61, 0.85) vs. 0.64 (0.52, 0.76), CRP (mg/L): 21.4 (3.9, 52.9) vs. 5.6 (2.4, 14.0), D-dimer (mg/L): 0.2 (0.2, 0.5) vs. 0.2 (0.1, 0.3), LDH (μmol×s×L): 4.49 (3.59, 5.19) vs. 3.12 (2.77, 4.90)]; at the same time, more traditional Chinese medicine was used in the patients with gastrointestinal symptoms [65.1% (28/43) vs. 40.5% (15/37), all P < 0.05]. In addition, 14 cases of 18 patients with cardiovascular diseases presented with poor appetite, 7 patients had nausea and vomiting symptoms. All of the 3 patients with chronic kidney disease presented with poor appetite, nausea and vomiting, and 2 of them had diarrhea.

Conclusions: The gastrointestinal symptoms in patients with COVID-19 are common. Whether it is caused by the virus or related drugs, diet and mental conditions, clinicians should analyze the causes of these symptoms timely, and then provide a better treatment for patients with COVID-19.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200406-00411DOI Listing
April 2020

Highly selective removal of Ni(II) from plating rinsing wastewaters containing [Ni-xNH-yPO] complexes using N-chelating resins.

J Hazard Mater 2020 11 20;398:122960. Epub 2020 May 20.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, PR China. Electronic address:

Inorganic complexants, such as ammonia (AA) and pyrophosphate (PP), are often present alongside heavy metal ions in alkaline plating rinsing wastewater. We investigated the removal capacity of Ni(II) from waters containing [Ni-xNH-yPO] complexes by chelating and ion-exchange resins in sole and dual-ligand systems. D463 (containing iminodiacetic groups) and PAMD (possessing polyamine groups) exerted superior performance under all conditions. Ni(II) adsorption on D463 decreased with AA and PP by 10.3% and 64.4%, respectively. Conversely, the adsorption on PAMD increased by 57.3% and 75.8%, respectively. PAMD exhibited high selectivity toward anionic [Ni-PP] species over free PP. More Ni(II) was captured by PAMD in the dual-ligand systems than sole systems, while the case for D463 was opposite. As confirmed by species tracking and DFT/XPS analyses, complexes breaking-Ni capture was the dominant mechanism for D463, while the dual-site (non-charged and protonated amines) interactions with NiPO on PAMD promoted its adsorption. The tandem combination D463-PAMD was the optimal mode to remove the most Ni(II). The actual wastewater test demonstrated that >210 BV effluent met the limit of 0.1 mg Ni(II)/L and the eluent contained 15 g Ni(II)/L. This study guides the application of chelating adsorption processes in the advanced treatment of plating rinsing wastewaters.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122960DOI Listing
November 2020

Resveratrol Nanoparticle Complex: Potential Therapeutic Applications in Myocardial Ischemia Reperfusion Injury.

J Biomed Nanotechnol 2020 Mar;16(3):382-389

Resveratrol (RES) is a natural non-flavonoid polyphenol with cardioprotective activities, antioxidant, antiplatelet, and antiinflammatory. However, its low aqueous solubility, chemical stability, and oral bioavailability, as well as a short circulation half-life greatly limit its clinical applications. To overcome these limitations of RES, we synthesized a methoxy poly(ethylene glycol)--oligomerization(D, L-Leucine) (mPEG--O(D, L-Leu)) nanoparticle (NP) as the carrier of RES and evaluated the myocardial-protective effectiveness of this RES/NP complex in rat myocardial ischemia-reperfusion injury models. We gauged the characterization of the NP through proton nuclear magnetic resonance spectroscopy, gel permeation chromatography, transmission electron microscope, and Fourier transform infrared spectroscopy and then loaded RES on the nanocarrier by hydrophobic interactions under physiological pH to extend the release time of RES and prolong its circulation half-life. Subsequently, we used rat cardiomyocytes (H9C2 cells) and rat MI/RI model to investigate the relationship between drug composition and myocardial preservation properties. It was found that RES was encapsulated quickly and efficiently, and displayed an effectual loading-capacity and sustained-release. Anti-MI/RI effect of the RES/NP complex was found satisfactory in rat models using free RES as the control. This study suggested that NP may prove to be a potent nanocarrier to augment the pharmacotherapy of RES against MI/RI.
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http://dx.doi.org/10.1166/jbn.2020.2900DOI Listing
March 2020

Low-dose corticosteroid therapy does not delay viral clearance in patients with COVID-19.

J Infect 2020 07 11;81(1):147-178. Epub 2020 Apr 11.

Department of Intensive Care Unit, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui Province 230001, China; Department of Intensive Care Unit, The Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui Province 230001, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2020.03.039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151466PMC
July 2020

First case of COVID-19 in a patient with multiple myeloma successfully treated with tocilizumab.

Blood Adv 2020 04;4(7):1307-1310

Department of Hematology, The First Affiliated Hospital of University of Science and Technology of China (USTC), Hefei, China.

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http://dx.doi.org/10.1182/bloodadvances.2020001907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160284PMC
April 2020

pH-sensitive magnetic drug delivery system via layer-by-layer self-assembly of CS/PEG and its controlled release of DOX.

J Biomater Sci Polym Ed 2020 06 16;31(8):1057-1070. Epub 2020 Mar 16.

School of Chemistry and Environmental Engineering, Jiangsu University of Technology, Changzhou, China.

Cancer is one of the biggest killers threat to human life and health and is still difficult to treat mainly due to the lack of targeted drug delivery stages and limitations associated with delivering drugs to targeted cancer tissues. The goal of this work was to develop a magnetic, pH-sensitive formulation for targeted delivery of chemotherapeutic agents to cancer cells. Then the functional drug delivery system (FeO@CS/PEG-DOX) was synthesized by the layer-by-layer (LbL) self-assembly technique. And the drug loading content is calculated to be 19.1%. In addition, the FeO@CS/PEG-DOX exhibited excellent pH-sensitivity, 73.1% DOX was released within 48 h at pH 4.0. Furthermore, all the release behaviors fit the Higuchi model very well and the dissolution of CS/PEG layers played a key role on DOX release from FeO@CS/PEG-DOX. The results of toxicity analysis in human liver hepatocellular carcinoma cells (HepG2) revealed that FeO@CS/PEG-DOX exhibited high anti-tumor activity, while the FeO@CS/PEG nanocomposites were practically non-toxic. Therefore, all the results demonstrated that the FeO@CS/PEG-DOX could have an important impact on the development of targeted intracellular delivery nanodevices for cancer therapy.
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http://dx.doi.org/10.1080/09205063.2020.1740963DOI Listing
June 2020

Treatment and posttreatment effects of a novel magnetic palatal expansion appliance with reactivation system in dogs.

Am J Orthod Dentofacial Orthop 2020 Feb;157(2):194-204

Department of Orthodontics, Affiliated Stomatological Hospital of Nanchang University, Stomatological and Biomedical Key Laboratory of Jiangxi Province, Nanchang, Jiangxi, China. Electronic address:

Introduction: This study aimed to compare the effects of a novel magnetic palatal expansion appliance (MPEA) during the expansion and maintenance period with that of a screw expansion appliance.

Methods: Based on previous research, the MPEA had a reactivation system that was modified for a broader working range and more stable expansion. Thirty-six male beagle dogs were assigned to a magnetic expansion (ME; n = 12), screwed expansion (SE; n = 12) or control (n = 12) group. Half of the dogs from each group were evaluated only during 5 weeks of activation, whereas the rest were evaluated for 5 weeks of activation and 8 additional weeks of retention. Nonmagnetic metal marking implants were implanted on both sides of the midpalatal suture of all dogs. Three-dimensional assessment of treatment and posttreatment dental and skeletal effects were conducted using cone-beam computed tomography. The width of the midpalatal suture, mineralization and deposition rate of bone, and fluorescence integral optical density were calculated during the expansion and retention periods using tetracycline fluorescence labeling.

Results: There were increases in the value of all cone-beam computed tomography parameters in the SE and ME groups during the expansion period, and the increase was significantly greater than that of the control group (P <0.01). However, there was no significant difference in the values of any parameters during the retention period. The width of the midline sutures, mineralization and deposition rate of bone, and integral optical density in the 2 experimental groups were significantly higher than those of the control group (P <0.01), and there was no significant difference between the SE and ME groups. After the retention period, the values of all tetracycline fluorescence evaluation parameters of the experimental groups decreased significantly.

Conclusions: The novel MPEA with a reactivation system was able to expand the midpalatal suture effectively. Dental and skeletal expansion effects are similar to those of the screw expansion appliance. Wearing the appliance as a retainer can effectively maintain the expansion effect. The new bone formation rate was accelerated during the expansion process and decreased to normal levels during the retention period.
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http://dx.doi.org/10.1016/j.ajodo.2019.03.020DOI Listing
February 2020

Clinical Characteristics and Prognosis of Influenza B Virus-Related Hospitalizations in Northern China during the 2017-18 Influenza Season: A Multicenter Case Series.

Biomed Res Int 2019 7;2019:8756563. Epub 2019 Nov 7.

Department of Emergency, Second Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province 050000, China.

Background: By weekly monitoring of China's influenza situation, Chinese National Influenza Center observed that the 2017-18 season was predominated by influenza B virus (IBV)/Yamagata. No studies regarding hospitalizations in adults with IBV infections have been performed. We aimed to describe the clinical characteristics of hospitalized patients with IBV infection in northern China.

Methods: In this multicenter and retrospective study, we reviewed all consecutive adult patients with confirmed IBV infections at two level A tertiary teaching hospitals in northern China during the 2017-18 influenza season. Patients' clinical and diagnostic findings, as well as administered treatments and mortality data, were analyzed.

Results: A total of 573 patients with a confirmed diagnosis of IBV infection were identified, of whom 22 cases were analyzed because of IBV-related hospitalization. Most patients were admitted to the intensive care unit (ICU) and had at least one underlying disease. The total in-hospital mortality was 27.3%. An elevated initial pneumonia severity index score, elevated direct bilirubin values, and lower platelet levels were associated with mortality (=0.020, 0.013, and 0.049, respectively). The quick development of bilateral diffuse alveolar infiltrates was the most common imaging characteristics, following consolidation and pleural effusion(s). Risk factors such as HIV infection, pregnancy, underlying medical conditions, coinfections, and treatment delays were not associated with mortality.

Conclusions: IBV should not be neglected because of its significant mortality. The elderly and patients with comorbidities, such as hypertension, diabetes, and connective tissue diseases, are more likely to have severe IBV-related pneumonia. Higher heart rates, direct bilirubin levels, initial PSI scores, and lower platelet levels are correlated with hospital mortality. Increased uptake in tetravalent influenza vaccine should be very helpful in preventing future cases of IBV hospitalizations.
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http://dx.doi.org/10.1155/2019/8756563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885173PMC
April 2020

Combined Ischemic Preconditioning and Resveratrol Improved Bloodbrain Barrier Breakdown Hippo/YAP/TAZ Signaling Pathway.

CNS Neurol Disord Drug Targets 2019 ;18(9):713-722

Department of Neurology, The First Affiliated Hospital of Xiamen University, Xiamen, China.

Background: Transient Ischemia/Reperfusion (I/R) is the main reason for brain injury and results in disruption of the Blood-Brain Barrier (BBB). It had been reported that BBB injury is one of the main risk factors for early death in patients with cerebral ischemia. Numerous investigations focus on the study of BBB injury which have been carried out.

Objective: The objective of this study was to investigate the treatment function of the activation of the Hippo/Yes-Associated Protein (YAP) signaling pathway by combined Ischemic Preconditioning (IPC) and resveratrol (RES) before brain Ischemia/Reperfusion (BI/R) improves Blood-Brain Barrier (BBB) disruption in rats.

Methods: Sprague-Dawley (SD) rats were pretreated with 20 mg/kg RES and IPC and then subjected to 2 h of ischemia and 22 h of reperfusion. The cerebral tissues were collected; the cerebral infarct volume was determined; the Evans Blue (EB) level, the brain Water Content (BWC), and apoptosis were assessed; and the expressions of YAP and TAZ were investigated in cerebral tissues.

Results: Both IPC and RES preconditioning reduced the cerebral infarct size, improved BBB permeability, lessened apoptosis, and upregulated expressions of YAP and transcriptional co-activator with PDZ-binding motif (TAZ) compared to the Ischemia/Reperfusion (I/R) group, while combined IPC and RES significantly enhanced this action.

Conclusion: combined ischemic preconditioning and resveratrol improved blood-brain barrier breakdown via Hippo/YAP/TAZ signaling pathway.
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http://dx.doi.org/10.2174/1871527318666191021144126DOI Listing
September 2020

Chain Substituted Cannabilactones with Selectivity for the CB2 Cannabinoid Receptor.

Molecules 2019 Oct 1;24(19). Epub 2019 Oct 1.

Center for Drug Discovery and Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.

In earlier work, we reported a novel class of CB2 selective ligands namely cannabilactones. These compounds carry a dimethylheptyl substituent at C3, which is typical for synthetic cannabinoids. In the current study with the focus on the pharmacophoric side chain at C3 we explored the effect of replacing the C1'--dimethyl group with the bulkier cyclopentyl ring, and, we also probed the chain's length and terminal carbon substitution with bromo or cyano groups. One of the analogs synthesized namely 6-[1-(1,9-dihydroxy-6-oxo-6-benzo[]chromen-3-yl) cyclopentyl] hexanenitrile (AM4346) has very high affinity ( = 4.9 nM) for the mouse CB2 receptor (mCB2) and 131-fold selectivity for that target over the rat CB1 (rCB1). The species difference in the affinities of AM4346 between the mouse (m) and the human (h) CB2 receptors is reduced when compared to our first-generation cannabilactones. In the cyclase assay, our lead compound was found to be a highly potent and efficacious hCB2 receptor agonist (EC = 3.7 ± 1.5 nM, = 89%). We have also extended our structure-activity relationship (SAR) studies to include biphenyl synthetic intermediates that mimic the structure of the phytocannabinoid cannabinodiol.
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http://dx.doi.org/10.3390/molecules24193559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804212PMC
October 2019

Preconditioning with endoplasmic reticulum stress alleviated heart ischemia/reperfusion injury via modulating IRE1/ATF6/RACK1/PERK and PGC-1α in diabetes mellitus.

Biomed Pharmacother 2019 Oct 2;118:109407. Epub 2019 Sep 2.

Xiamen Diabetes Institute, The First Affiliated Hospital, Xiamen University, Xiamen, 361000, China. Electronic address:

The purpose of this study was to observe the functions of preconditioning with endoplasmic reticulum stress (ERS) whether alleviated heart ischemia/reperfusion injury (HI/RI) via modulating IRE1/ATF6/RACK1/PERK and PGC-1α expressions in diabetes mellitus (DM) or not. Diabetic rats were pretreated with 0.6 mg/kg tunicamycin (TM, 0.6 mg/kg tunicamycin was administered via intraperitoneal injection 30 minutes prior to the I/R procedures), and then subjected to 45 minutes of ischemia and 3 hours of reperfusion. Blood and myocardial tissues were collected, myocardial pathological injuries were investigated, serum creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT) levels were measured, left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), maximum rate of left ventricular pressure rise (+dp/dt) and maximum rate of left ventricular pressure drop (-dp/dt) were evaluated, reactive oxygen species (ROS) and caspase-3 levels were observed, ΔΨm level and ROS expression were measured, and activated transcript factor 6 (ATF6), receptor for activated C kinase 1 (RACK1), PRK-like ER kinase (PERK), glucose regulated protein 78 (GRP78) and peroxisome proliferator-activated receptor γ co-activator 1-α (PGC-1α) expressions were assessed. The TM ameliorated the pathological damages, reduced myocardial oxidative stress damages, restrained apoptosis, and upregulated the expressions of ATF6, RACK1, PERK, GRP78 and PGC-1α compared with those of the ischemia/reperfusion (I/R) group in DM. This study suggested the preconditioning with endoplasmic reticulum stress (TM) strategy that could enhance protection against HI/RI in DM in clinical myocardial diseases.
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http://dx.doi.org/10.1016/j.biopha.2019.109407DOI Listing
October 2019

Crystal-to-Gel Transformation Stimulated by a Solid-State E→Z Photoisomerization.

Angew Chem Int Ed Engl 2019 Oct 13;58(43):15429-15434. Epub 2019 Sep 13.

Department of Chemistry, University of California, Riverside, 501 Big Springs Road, Riverside, CA, 92521, USA.

The molecule (E)-(5-(3-anthracen-9-yl-allylidene)-2,2-dimethyl-[1,3] dioxane-4,6-dione) (E-AYAD) undergoes E→Z photoisomerization. In the solid state, this photoisomerization process can initiate a physical transformation of the crystal that is accompanied by a large volume expansion (ca. 10 times), loss of crystallinity, and growth of large pores. This physical change requires approximately 10 % conversion of the E isomer to the Z isomer and results in a gel-like solid with decreased stiffness that still retains its mechanical integrity. The induced porosity allows the expanding gel to engulf superparamagnetic nanoparticles from the surrounding liquid. The trapped superparamagnetic nanoparticles impart a magnetic susceptibility to the gel, allowing it to be moved by a magnetic field. The photoinduced phase transition, starting with a compact crystalline solid instead of a dilute solution, provides a new route for in situ production of functional porous materials.
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http://dx.doi.org/10.1002/anie.201907454DOI Listing
October 2019

Simvastatin Alleviates Intestinal Ischemia/Reperfusion Injury by Modulating Omi/HtrA2 Signaling Pathways.

Transplant Proc 2019 Oct 24;51(8):2798-2807. Epub 2019 Jul 24.

Cardiovascular Medicine, Shanxi Cardiovascular Disease Hospital, Taiyuan, Shanxi, China. Electronic address:

Purpose: The objective of this research was to survey the therapeutic action of simvastatin (Sim) on intestinal ischemia/reperfusion injury (II/RI) by modulating Omi/HtrA2 signaling pathways.

Methods: Sprague Dawley rats were pretreated with 40 mg/kg Sim and then subjected to 1 hour of ischemia and 3 hours of reperfusion. The blood and intestinal tissues were collected, pathologic injury was observed, the contents of serum tumor necrosis factor-α and interleukin-6 (IL-6) were estimated, and superoxide dismutase, methane dicarboxylic aldehyde, and cysteinyl aspartate specific proteinase-3 (caspase-3) levels, as well as the expressions of Omi/HtrA2 and caspase-3, were measured in the intestinal tissues.

Results: Sim preconditioning mitigated the damnification of intestinal tissues by decreasing oxidative stress, inflammatory damage, and apoptosis and downregulating the expression of Omi/HtrA2 compared to the ischemia/reperfusion group, while Sim+Ucf-101 significantly augmented this effect.

Conclusion: These results suggest that Sim may alleviate intestinal ischemia/reperfusion injury by modulating Omi/HtrA2 signaling pathways.
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http://dx.doi.org/10.1016/j.transproceed.2019.04.076DOI Listing
October 2019