Publications by authors named "Fei Ma"

681 Publications

Isolation and characterization of a podovirus infecting the opportunist pathogen Vibrio alginolyticus and Vibrio parahaemolyticus.

Virus Res 2021 Jun 10:198481. Epub 2021 Jun 10.

Beijing University of Chemical Technology, China. Electronic address:

Bacterial infections have a negative impact on both animal husbandry industry and medicine, and increasing bacterial drug resistance exacerbates this adverse impact. Phages show promise as an alternative to drugs against drug-resistant bacteria. In this study, a novel virulent bacteriophage (phage) vB_ValP_IME234 against Vibrio alginolyticus and Vibrio parahaemolyticus was isolated from freshwater in Beijing, China. Phage vB_ValP_IME234 had an isometric head (59 nm in diameter) and a short tail (10 nm long), belonging to Podoviridae family. Its complete genome is liner double-stranded DNA (dsDNA) with a GC content of 41.6% while encoding 61 putative proteins. Three transfer RNA (tRNA) and no lysogenic gene was detected. vB_ValP_IME234 had a polyvalent infectivity, a burst of 390 PFU/cell, and is stable under different temperatures (4°C to 50°C) and pH (6.0 to 10.0) values. Host range test showed that vB_ValP_IME234 has the ability to infect seven strains of Vibrio in total. Phylogenetic analyses based on terminase and capsid suggested that this phage had a close relationship with Vibrio phages. These results indicate that vB_ValP_IME234 could be used as a potential biocontrol agent against V. alginolyticus strains.
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http://dx.doi.org/10.1016/j.virusres.2021.198481DOI Listing
June 2021

Dynamic Stark Effect in Two-Dimensional Spectroscopy Revealing Modulation of Ultrafast Charge Separation in Bacterial Reaction Centers by an Inherent Electric Field.

J Phys Chem Lett 2021 Jun 7;12(23):5526-5533. Epub 2021 Jun 7.

Department of Biophysics, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.

Despite extensive study, mysteries remain regarding the highly efficient ultrafast charge separation processes in photosynthetic reaction centers (RCs). In this work, transient Stark signals were found to be present in ultrafast two-dimensional electronic spectra recorded for purple bacterial RCs at 77 K. These arose from the electric field that is inherent to the intradimer charge-transfer intermediate of the bacteriochlorophyll pair (P), PP. By comparing three mutated RCs, a correlation was found between the efficient formation of PP and a fast charge separation rate. Importantly, the energy level of P* was changed due to the Stark shift, influencing the driving force for P* → PB electron transfer and hence its rate. Furthermore, the orientation and amplitude of the inherent electric field varied in different ways upon different mutation, leading to contrasting changes in the rates. This mechanism of modulation provides a solution to a long-lasting inconsistency between experimental observations and activation energy theory.
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http://dx.doi.org/10.1021/acs.jpclett.1c01059DOI Listing
June 2021

Effects of sulfometuron-methyl on zebrafish at early developmental stages.

Ecotoxicol Environ Saf 2021 Jun 1;220:112385. Epub 2021 Jun 1.

Ganzhou Key Laboratory for Drug Screening and Discovery, School of Geography and Environmental Engineering, Gannan Normal University, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Ji'an 343009, Jiangxi, China. Electronic address:

Sulfometuron methyl (SM) is a widely used herbicide and thus leading to accumulation in the environment. The toxicity assessments of SM in model organisms are currently rare. In the present study, zebrafish were utilized for evaluating the detrimental effects of SM in aquatic vertebrates. Zebrafish embryos were exposed to 0, 10, 20, and 40 mg/L SM from 5.5 to 72 h post-fertilization (hpf), respectively. Consequently, SM exposure resulted in increasing the mortality rate and reducing hatching rate in larval zebrafish at 10, 20, and 40 mg/L SM-treated groups. The reduced numbers of immune cells (neutrophils and macrophages) were observed after SM exposure by a dose-dependent manner. The inflammatory responses (TLR4, MYD88, IL-1β, IL-6, IL-8, IFN-γ, IL-10, and TGF-β) were measured to estimate immune responses. Anti-inflammatory factors (IL-10 and TGF-β) were down-regulated in all the treated groups and significantly altered at 40 mg/L exposure group. Additionally, behavioral tests suggested that SM treatment significantly increased the total distance, average speed, and maximum acceleration of larval zebrafish during light-dark transition and subsequently enzymology test displayed the same trend to locomotor behaviors. The content significantly increased in oxidative stress, as reflected in ROS level in all the treated groups. The numbers of cell apoptosis were significantly increased at 20, and 40 mg/L and the highest concentration group induced the substantial increment (P < 0.001) of apoptosis-related genes including p53, Bax/Bcl-2, caspase-9, and caspase-3. In summary, our results demonstrated that exposure to SM caused toxicity of development, immune system, locomotor behavior, oxidative stress, and cell apoptosis at the early developmental stages of zebrafish.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112385DOI Listing
June 2021

Using a mixture of local bone dust and morselized bone as graft materials in single- and double-level ACDF.

BMC Musculoskelet Disord 2021 Jun 2;22(1):510. Epub 2021 Jun 2.

Department of Orthopedics, Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Sichuan, 646000, Luzhou, China.

Background: Using a cage filled with local bone in anterior cervical discectomy and fusion (ACDF) can eliminate morbidities associated with autograft harvest from the iliac crest while achieving high fusion rates. However, there is still no consensus regarding the methods for using local bone grafts. This retrospective study was performed to compare the clinical and radiological outcomes of using a mixture of bone dust and morselized bone versus morselized bone alone in ACDF.

Methods: A retrospective study of 228 patients affected by cervical degenerative disease who had undergone single- or double-level ACDF between January 2014 and June 2018 was performed. Nanohydroxyapatite/polyamide-66 (n-HA/PA66) combined with morselized bone was used in 111 patients (group A: single-level ACDF in 51 patients and double-level ACDF in 60 patients), whereas the n-HA/PA66 cage combined with a mixture of bone dust and morselized bone was used in 117 patients (group B: single-level ACDF in 58 patients and double-level ACDF in 59 patients). The fusion rate, extent of cage subsidence, fusion segmental height (FSH), C2-7 lordosis, segmental sagittal alignment (SSA), 10-point visual analog scale (VAS) score, and Neck Disability Index (NDI) were compared between the two groups.

Results: The VAS score and NDI were significantly reduced after the operation in group A and group B. At the final follow-up, the fusion rate was 90.2 % (46/51) and 94.8 % (55/58) in patients treated with single-level ACDF in group A and group B, respectively (p > 0.05). In patients treated with double-level ACDF, bone fusion was achieved in 52 patients (86.7 %) in group A and 55 patients (93.2 %) in group B (p > 0.05). The fusion rate of single- and double-level ACDF was higher in patients in group B than those in group A at the 3-month, 6-month and 12-month follow-ups (p < 0.05). The extent of cage subsidence after single- and double-level ACDF was lower in patients in group B (1.5 ± 0.5 mm and 2.3 ± 0.8 mm, respectively) than in those in group A (1.8 ± 0.7 mm and 2.9 ± 1.4 mm, respectively) (p < 0.05). There was no significant difference between the two groups in the C2-7 lordosis, FSH, SSA, VAS score, or NDI before or after the operation (p > 0.05).

Conclusions: Using a mixture of local bone dust and morselized bone as cage-filling materials yielded comparably good clinical outcomes as using morselized bone alone in single- and double-level ACDF. However, the mixture graft of bone dust and morselized bone was more beneficial in promoting early fusion and reducing cage subsidence.
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http://dx.doi.org/10.1186/s12891-021-04394-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173926PMC
June 2021

The association between cardiovascular risk burden and cognitive function among the old: a 9-year longitudinal cohort study.

Eur J Neurol 2021 Jun 2. Epub 2021 Jun 2.

Department of Epidemiology & Biostatistics, School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Cardiovascular risk burden in midlife has been linked to cognitive decline in later life, but whether this association still exist in older cohorts is unclear.

Methods: We investigated the association between the cardiovascular risk score and cognitive function using the 9-year follow-up data. The risk score evaluated algorithms were from Chinese guidelines on prevention and treatment of dyslipidemia in adults (2016 revised), which was assessed at baseline and categorized into tertiles (low, middle, and high). Full intelligence quotient (FIQ), verbal intelligence quotient (VIQ) and performance intelligence quotient (PIQ) were assessed with Wechsler adult intelligence scale-Chinese revised (WAIS-RC) at follow-ups. Data were analyzed using linear mixed-effects model.

Results: A total of 924 participants (mean age: 78.06 ± 7.58 years) were included in our study. In all participants, risk score ranged from 0.02 to 0.55 (mean score: 0.16 ± 0.08). Compared with the low tertile, higher risk score was associated with lower FIQ (β:-0.094, 95% confidence interval [CI]: -0.181, -0.007) and VIQ (β:-0.100; 95% CI: -0.192, -0.007) over the follow-up. There is more significant association between higher risk score and lower FIQ among female (β:-0.263; 95% CI: -0.462, -0.065) and VIQ (β:-0.268; 95% CI: -0.478, -0.057).

Conclusions: The higher cardiovascular risk score was associated with lower FIQ and VIQ. Higher cardiovascular risk burden increased the risk of cognition impairment and accelerated its progression over time. This study has implications for early detection of cognition impairment.
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http://dx.doi.org/10.1111/ene.14947DOI Listing
June 2021

What is the difference in pedicle morphology of the fifth lumbar vertebra between isthmic and degenerative L5-S1 spondylolisthesis? An anatomic study of 328 patients via multi-slice spiral computed tomography.

Eur Spine J 2021 May 28. Epub 2021 May 28.

Department of Orthopaedics, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Lu Zhou City, China.

Purpose: The purpose of this study was to investigate and determine whether there are differences in L5 pedicles morphology between isthmic and degenerative L5-S1 spondylolisthesis.

Methods: One hundred and nineteen patients with isthmic spondylolisthesis and 45 patients with degenerative spondylolisthesis at L5-S1 were enrolled in the IS group and DS group, respectively, and 164 lumbar disc herniation patients without spondylolysis or spondylolisthesis were classified into the normal (NL) group. A series of pedicle parameters of the fifth lumbar vertebra, including pedicle length (PL), pedicle width (PW), pedicle screw trajectory length (PSTL), pedicle height (PH), and the pedicle camber angle (PCA), were measured using multi-slice spiral computed tomography (MSCT). The slip distance of the L5 vertebra was measured on radiography, and the percentage of slip was also recorded.

Results: The pedicles of the fifth lumbar vertebra were shorter and wider, and the PCA was larger in the IS group compared to the DS group and NL group. On the contrary, the pedicles in the DS group were elongated and thinner, and the PCA was smaller. The pedicle parameters of PL were significantly positively correlated with the percentage of slip in the DS group, but PW and PCA were negatively correlated with the percentage of slip. There was no correlation between the percentage of slip and L5 pedicle parameters in the IS group.

Conclusions: The L5 pedicles morphology in L5-S1 isthmic spondylolisthesis shows abduction, shortness, and width, while that in the degenerative spondylolisthesis shows adduction, lengthening, and thinning compared with the normal populations. The morphology changes may be the result of pedicle stress remodelling in the development of spondylolisthesis, which should be taken into consideration when placing at the insertion of pedicle screws.
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http://dx.doi.org/10.1007/s00586-021-06884-3DOI Listing
May 2021

Risk Factors and Survival of Patients With Liver Metastases at Initial Metastatic Breast Cancer Diagnosis in Han Population.

Front Oncol 2021 11;11:670723. Epub 2021 May 11.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

The risk factors for morbidity and mortality in patients with breast cancer liver metastases (BCLM) upon initial metastatic breast cancer (MBC) diagnosis have not been adequately identified in Han population. Data of 3,161 female patients who were initially diagnosed with MBC from December 1991 to September 2019 and treated in the China National Cancer Center were extracted and a total of 2,263 MBC patients were included in our study, among which 550 patients had liver metastases. Multivariable logistic regression was performed to identify risk factors for the presence of liver metastases at initial MBC diagnosis. Univariable and multivariable Cox proportional hazards regression analyses were conducted to determine prognostic factors for the survival of BCLM patients. Patients with hormone receptor (HR)-negative, human epidermal growth factor receptor 2 (HER2)-positive (35.0% of the entire population) subtype had the highest incidence of liver metastases. stage IV breast cancer, HR-/HER2+ and HR+/HER2+ subtypes were associated with higher odds of liver metastases and patients with lung metastases had lower risk of liver metastases at initial MBC diagnosis. The median overall survival of BCLM patients was 31.4 months and BCLM patients with HR+/HER2- subtype had the longest survival of 38.2 months. Older age, worse performance status, later stage of initial breast cancer, triple-negative subtype and lung metastases were significantly associated with a poorer prognosis in BCLM patients. Our study offers insights into the incidence and prognosis of BCLM patients at initial MBC diagnosis in Han population.
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http://dx.doi.org/10.3389/fonc.2021.670723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147724PMC
May 2021

[Characteristics and influence factors in leaf and soil carbon stable isotopes of .]

Ying Yong Sheng Tai Xue Bao 2021 May;32(5):1744-1752

School of Ecology and Environment, Ningxia University, Yinchuan 750021, China.

In order to explore the relationship of plant carbon (C) cycling with its habitat in the high-altitude regions, a leguminous shrub, , that mainly distributed in those areas was studied. We collected leaf and soil samples of from 35 sites along an east-west transect across the alpine regions of China. We measured leaf carbon stable isotope (δC), soil δC, and the difference between leaf and soil δC (ΔδC) of each sampling site. We further analyzed the effects of climatic factors, leaf and soil elements on leaf δC, soil δC and ΔδC. Results showed that leaf δC ranged from -30.9‰ to -27.1‰, with a mean value of -28.4‰, soil δC ranged from -26.2‰ to -23.2‰, with a mean value of -25.3‰, and ΔδC ranged from 2.0‰ to 7.7‰, with a mean value of 3.1‰. δC values of leaf was significantly lower than that of soil. Soil δC initially decreased and subsequently increased with increasing leaf δC. Leaf δC was negatively correlated with growing season temperature and leaf C content. Soil δC was negatively correlated with relative humidity and mean temperature of the warmest month, and was positively correlated with soil carbon:nitrogen (C:N). Soil δC firstly decreased and subsequently increased with soil C content. ΔδC was positively correlated with leaf C content, soil C content, and soil C: N. Climatic factors directly affected leaf δC and ΔδC, and indirectly affected leaf δC, soil δC and ΔδC through their effects on leaf and soil elements. The C cycle of . was affected by climatic factors, leaf and soil elements in the alpine regions.
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http://dx.doi.org/10.13287/j.1001-9332.202105.004DOI Listing
May 2021

Phase II study of apatinib in combination with oral vinorelbine in heavily pretreated HER2-negative metastatic breast cancer and clinical implications of monitoring ctDNA.

Cancer Biol Med 2021 May 26. Epub 2021 May 26.

Unit of Medical Oncology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma 00176, Italy.

Objective: Apatinib is an oral TKI targeting VEGFR-2. Single-agent apatinib treatment has been shown to produce an objective response in patients with pretreated mBC. Oral vinorelbine also holds promise as a treatment of choice in patients with mBC. This study aimed to investigate the efficacy and safety of the oral vinorelbine-apatinib combination in patients with pretreated mBC. In addition, we detected gene variants in ctDNA to explore the therapeutic implications.

Methods: This study enrolled patients with HER2-negative mBC who were pretreated with anthracycline/taxanes. Patients were treated with apatinib at 500 mg/425 mg daily plus oral vinorelbine 60 mg/m on days 1, 8, and 15 of every cycle (3 weeks). The primary endpoint was PFS. The secondary endpoints were ORR, CBR, OS, and safety. Patients eligible for ctDNA detection were evaluated before and during treatment.

Results: Forty patients were enrolled. The median PFS was 5.2 months (95% CI, 3.4-7.0 months), and the median OS was 17.4 months (95% CI, 8.0-27.0 months). The ORR was 17.1% (6/35), and the CBR was 45.7% (16/35). The most common AEs included gastrointestinal reaction, myelosuppression, and hypertension. In 20 patients, ctDNA was detected at baseline and during treatment. A significant difference was found in PFS for undetected detected baseline ctDNA (13.9 months 3.6 months, = 0.018).

Conclusions: All-oral therapy with apatinib plus vinorelbine displayed objective efficacy in patients with heavily pretreated HER2-negative mBC, with acceptable and manageable toxicity profiles. Patients with no gene variant detected and lower variant allele frequencies in ctDNA at baseline showed longer PFS.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0418DOI Listing
May 2021

Self-assembled CoSe-FeSe heteronanoparticles along the carbon nanotube network for boosted oxygen evolution reaction.

Nanoscale 2021 Jun;13(21):9651-9658

State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu 610054, PR China.

Water electrolysis is a significant alternative technique to produce clean hydrogen fuel in order to replace environmentally destructive fossil fuel combustion. However, the sluggish oxygen evolution kinetics makes this process vulnerable as it requires relatively high overpotentials. Hence, significantly effective electrocatalysts are necessary to access the water-oxidation process at a low overpotential to make this process industrially viable. Therefore, in order to reduce the energy barrier, we developed bimetallic CoSe2-FeSe2 heteronanoparticles along the carbon nanotube network (CoSe2-FeSe2/CNT) via a facile selenization strategy. Due to the unique assembly of highly conductive nanoparticles along the CNT network, the CoSe2-FeSe2/CNT displays an exceptionally good oxygen evolution (OER) activity; it requires 248 mV overpotential to reach a current density of 10 mA cm-2 (η10) with an ultra-low Tafel slope of 36 mV dec-1 and displays an overpotential of 1.59 V (η10) in the full water-splitting catalysis with the commercial Pt/C cathode. The high OER activity of CoSe2-FeSe2/CNT over the monometallic CoSe2/CNT and FeSe2/CNT electrocatalysts approve the synergistic interactions. Therefore, the superior performance is possibly ascribed to the unique porous nanoarchitecture and the strong coupling interactions between CoSe2 and FeSe2 heteronanoparticles on the conductive network. This study introduces an innovative approach to rationally design and fabricate cost-effective and highly proficient electrocatalysts for boosted OER performance.
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http://dx.doi.org/10.1039/d1nr01092aDOI Listing
June 2021

Laparoscopic-assisted versus open proximal gastrectomy with double-tract reconstruction for Siewert type II-III adenocarcinomas of esophago-gastric junction: a retrospective observational study of short-term outcomes.

J Gastrointest Oncol 2021 Apr;12(2):249-258

Department of Medical Oncology, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.

Background: Currently, the surgical approach to adenocarcinomas of esophago-gastric junction (AEG) remains controversial. Function-preserving gastric surgeries are becoming more popular, with proximal gastrectomy with double-tract anastomosis being one of the most important for AEG. Meanwhile, with the increasing use of laparoscopic techniques in the treatment of gastric cancer, the safety and effectiveness of laparoscopic-assisted proximal gastrectomy with double-tract anastomosis for Siewert type II-III AEG need to be further clarified.

Methods: Data of patients with Siewert type II/III AEG was collected at our center from October 2010 to December 2019 were retrospectively analyzed. 61 patients underwent open proximal gastrectomy with double-tract anastomosis (OPG-DT group) and 52 underwent laparoscopic-assisted proximal gastrectomy with double-tract anastomosis (LAPG-DT group). The clinical features, surgery, and short-term outcomes of patients in these 2 groups were collected to assess the safety and feasibility of LAPG-DT.

Results: A total of 113 patients were analyzed, there were 98 males and 15 females. No death during the operation. The differences in the number of lymph nodes, time to first flatus time to first eating, postoperative hospital stay, Additional analgesics were not statistically significant between two groups. Although the operative duration of LAPG-DT group was significantly longer than that of the OPG-DT group [(217±61) (161±14) min, P=0.000), while less blood loss and less stress in LAPG-DT group. Early and late postoperative complications were similar between two groups.

Conclusions: Although laparoscopic-assisted proximal gastrectomy with double-tract anastomosis requires long operative time, it is associated with less bleeding and milder stress. Therefore, it is a safe and feasible surgical method.
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http://dx.doi.org/10.21037/jgo-21-165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107614PMC
April 2021

Orphan drugs in different countries and development of new drugs to treat biliary tract cancer.

Intractable Rare Dis Res 2021 May;10(2):146-147

Department of Oncology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Biliary tract cancer (BTC), which includes cholangiocarcinoma and gallbladder carcinoma, is a rare malignancy. Due to its low incidence, drugs treating these diseases are scarce, so they can be considered orphan drugs. The main treatment choice for BTC is chemotherapy with gemcitabine or cisplatin or combined use of both, but patients fail to significantly benefit from established chemotherapy. Advancements in immunotherapy and targeted therapy will shed light on ways to improve clinical outcomes for patients with BTC. In conjunction, more new drugs will come onto the market. This article compares the conditions for development of orphan drugs in different countries and it describes several types of new drugs that were recently approved to treat BTC.
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http://dx.doi.org/10.5582/irdr.2021.01038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122311PMC
May 2021

The effects of infection on pregnancy-related diseases and fetal development in diabetes in pregnancy.

Ann Transl Med 2021 Apr;9(8):686

Endocrinology Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: This study aimed to determine the association of () infection with pregnancy-related diseases and fetal development in women with diabetes in pregnancy (DIP).

Methods: All the participants were recruited before 16 weeks of gestation. According to their medical history and the results of a 75-g oral glucose tolerance test at the 24 week of pregnancy, the participants were divided into a normal control group (NC group), a gestational diabetes mellitus group (GDM group), and a pre-pregnancy diabetes mellitus group (PGDM group). According to the results of an serum antibody detection test, each group was further divided into two subgroups: an positive subgroup ( subgroup) and an negative group ( subgroup). The incidences of pregnancy-related diseases, the fetal developmental status, and the newborn status were compared among the groups.

Results: This study recruited 356 pregnant women. The infection rates of type I were significantly higher in the GDM group and the PGDM group than in the NC group (χ=6.949, P=0.031). With the exception of the NC- subgroup, there were higher incidences of pregnancy-related diseases in the subgroups than in the subgroups (P0.05). Furthermore, the incidences of pregnancy-induced hypertension (PIH), preeclampsia, and premature delivery were significantly higher in the GDM- subgroup and the PGDM- subgroup than in the NC- subgroup (P<0.05). At the end of pregnancy, all 3 subgroups showed better fetal development than the subgroups (P<0.05), and the NC- subgroup showed better fetal development than the GDM- and PGDM- subgroups (P<0.05). Meanwhile, the PGDM- subgroup showed poor fetal development, even in the 2 trimester of pregnancy.

Conclusions: infection is extremely common in DIP. For women with DIP, infection with can increase the risks of pregnancy-related diseases and poor fetal development. screening and eradication therapy before pregnancy may aid in preventing pregnancy-related diseases and improve fetal development.
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http://dx.doi.org/10.21037/atm-21-1209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106047PMC
April 2021

The association between RAPSN methylation in peripheral blood and breast cancer in the Chinese population.

J Hum Genet 2021 May 6. Epub 2021 May 6.

Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, China.

DNA methylation in peripheral blood is associated with breast cancer (BC) but has mainly been studied in Caucasian populations. We investigated the association between blood-based methylation of receptor-associated protein of the synapse (RAPSN) and BC in Chinese population. The methylation levels of 12 RAPSN CpG sites were quantitatively evaluated by mass spectrometry in two case-control studies with 283 sporadic BC cases and 331 controls totally. The association was analyzed by logistic regression adjusted for covariants. The RAPSN methylation levels in patients with variant clinical characteristics were investigated by non-parametric tests. We found a significant association between BC and altered RAPSN methylation in blood in women at premenopausal and perimenopausal (age < 50 years old), but not in the elder women. This was approved by two independent case-control studies as well as by combining the subjects of the two studies (taken all subjects together, age < 50 years old, per 5% of methylation, odds ratio (OR) range from 1.17 to 1.30 for two CpG sites; OR = 0.75 for one CpG site; all p values < 0.02). This age-related RAPSN methylation was further modified by human epidermal growth factor receptor 2 (HER2) status (age < 50 years old, HER2 negative, per 5% of methylation, OR range from 1.27 to 1.48 for two CpG sites; OR = 0.76 for one CpG site; all p values < 0.02). We elucidated an association between BC and blood-based RAPSN methylation influenced by age and the status of HER2 in Chinese population.
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http://dx.doi.org/10.1038/s10038-021-00933-xDOI Listing
May 2021

Adsorptive removal of aflatoxin B from vegetable oils via novel adsorbents derived from a metal-organic framework.

J Hazard Mater 2021 06 23;412:125170. Epub 2021 Jan 23.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China; Laboratory of Quality and Safety Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China.

Vegetable oils are essential daily diet, but they are simply contaminated with aflatoxin B (AFB), a serious toxic compound to human health. Adsorption method due to the easy operation, high efficiency and low costing is set to become a main detoxification technique for AFB. Unfortunately, previous reported adsorbents were rarely used for detoxification in food industry since they cannot meet the criteria of large-scale production of edible oils. Metal-organic frameworks (MOFs) with unique textural properties could be favorable precursors for synthesis of advanced materials. In this research, three kinds of Cu-BTC MOF-derived porous materials were prepared by different carbonization temperature and characterized by XRD, SEM, FT-IR, and nitrogen adsorption-desorption techniques. Isotherm and kinetic studies on the adsorption behaviour of AFB onto the three porous carbonaceous materials have been systematically conducted. The results revealed that the porous carbonaceous materials could act as the excellent adsorbents that were of enough adsorption sites for AFB, mainly due to the hierarchical porous structure and large surface areas for the enhancement of adsorption capacity. Notably, the porous carbonaceous materials could not only remove more than 90% of AFB from real vegetable oils within 30 min, but also remain the treated oils at low cytotoxicity. Meanwhile, the detoxification process could little affect the quality of oils. Thus, the Cu-BTC MOF-derived porous carbonaceous materials with high efficiency, safe, practical and economic characteristics could be novel potential adsorbents used in the application of AFB removal from contaminated vegetable oils.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125170DOI Listing
June 2021

Analyzing master regulators and scRNA-seq of COVID-19 patients reveals an underlying anti-SARS-CoV-2 mechanism of ZNF proteins.

Brief Bioinform 2021 Apr 27. Epub 2021 Apr 27.

COVID-19 Research Center, Institute of Laboratory Medicine, Jinling Hospital, Nanjing University School of Medicine; Nanjing Clinical College of Southern Medical University, Nanjing, China.

Studies have demonstrated that both mortality and severe illness rates exist significant difference in different gender COVID-19 patients, but the reasons are still very mysterious to date. Here, we firstly find that the survival outcome of female patients is better to male patients through analyzing the 3044 COVID-19 cases. Secondly, we identify many important master regulators [e.g. STAT1/STAT2 and zinc finger (ZNF) proteins], in particular female patients can express more ZNF proteins and stronger transcriptional activities than male patients in response to SARS-CoV-2 infection. Thirdly, we discover that ZNF protein activity is significantly negative correlation with the SARS-CoV-2 load of COVID-19 patients, and ZNF proteins as transcription factors can also activate their target genes to participate in anti-SARS-CoV-2 infection. Fourthly, we demonstrate that ZNF protein activity is positive correlation with the abundance of multiple immune cells of COVID-19 patients, implying that the highly ZNF protein activity might promote the abundance and the antiviral activity of multiple immune cells to effectively suppress SARS-CoV-2 infection. Taken together, our study proposes an underlying anti-SARS-COV-2 role of ZNF proteins, and differences in the amount and activity of ZNF proteins might be responsible for the distinct prognosis of different gender COVID-19 patients.
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http://dx.doi.org/10.1093/bib/bbab118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135462PMC
April 2021

Safety and efficacy study of oral metronomic vinorelbine combined with trastuzumab (mNH) in HER2-positive metastatic breast cancer: a phase II trial.

Breast Cancer Res Treat 2021 Apr 25. Epub 2021 Apr 25.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No.17, Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.

Purpose: We conducted a single-arm prospective phase II trial to evaluate the efficacy and safety of oral metronomic vinorelbine combined with trastuzumab (mNH) in human epidermal growth factor receptor 2-positive (HER2-positive) metastatic breast cancer (MBC) patients.

Methods: HER2-positive MBC patients received oral vinorelbine 40 mg thrice a week and trastuzumab (loading dose of 8 mg/kg, followed by 6 mg/kg every 3 weeks) until disease progression, unacceptable toxicity, or consent withdrawal. The primary endpoints were objective response rate (ORR), clinical benefit rate (CBR; CR + PR + SD for ≥ 24 weeks). The secondary endpoints were progression-free survival (PFS), tolerability, and overall survival (OS).

Results: Twenty patients with HER2-positive MBC were enrolled, with a median of 1 prior chemotherapy regimens for MBC. Median age was 61.5 years (95% Confidence Interval (CI) 48.6-63.1). Visceral involvements presented in 14 patients (70.0%). ORR was 20.0%, and CBR was 75% with 4 PR (20.0%) and 11 SD (55.0%). The median PFS (mPFS) and median OS (mOS) were 7.4 months (95% CI 3.2-11.5) and 45.8 months (95%CI: not reached), respectively. The mPFS was 17.7 months (95%CI not reached) and 5.8 months (95%CI 5.6-5.9) in mNH as first-line and ≥ second-line therapy (log rank p = 0.03), respectively. The most common grade 1 adverse events (AEs) included nausea (15%), leukopenia (15%), ALT/AST elevation (15%), diarrhea (10%), and peripheral neuropathy (10%). Grade 2 adverse events included leukopenia (5%) and neutropenia (10%). No grade 3/4 AEs were observed.

Conclusions: Oral metronomic vinorelbine combined with trastuzumab is a well-tolerated and effective anti-tumor regimen for HER2-positive MBC.
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http://dx.doi.org/10.1007/s10549-021-06216-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070982PMC
April 2021

In Situ Construction of Mo C Quantum Dots-Decorated CNT Networks as a Multifunctional Electrocatalyst for Advanced Lithium-Sulfur Batteries.

Small 2021 Jun 23;17(23):e2100460. Epub 2021 Apr 23.

School of Electronic Science and Engineering, and State Key Laboratory of Electronic Thin Films and Integrated Devices, University of Electronic Science and Technology of China, Chengdu, 610054, P. R. China.

The slow redox kinetics during cycling process and the serious shuttle effect caused by the solubility of lithium polysulfides (LiPSs) dramatically hinder the practical application of Li-S batteries. Herein, a facile and scalable spray-drying strategy is presented to construct conductive polar Mo C quantum dots-decorated carbon nanotube (CNT) networks (MCN) as an efficient absorbent and electrocatalyst for Li-S batteries. The results reveal that the MCN/S electrode exhibits a high specific capacity of 1303.3 mAh g at 0.2 C, and ultrastable cycling stability with decay of 0.019% per cycle even at 1 C. Theoretical simulation uncovers that Mo C exhibits much stronger binding energies for S and Li S . The energy barrier for the conversion between Li S and Li S decreases from 1.02 to 0.72 eV when hybriding with Mo C. Furthermore, in situ discharge/charge-dependent Raman spectroscopy shows that long-chain Li S configuration is generated via S ring opening near the first plateaus at ≈2.36 V versus Li/Li and the S configuration in CNT/S electrode is maintained below the potential of ≈2.30 V versus Li/Li , indicating that the shuttle of soluble LiPSs happens during the whole discharge process. This work provides deep insights into the polar nanoarchitecture design and scalable fabrication for advanced Li-S batteries.
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http://dx.doi.org/10.1002/smll.202100460DOI Listing
June 2021

Gender-specific prevalence and influencing factors of depression in elderly in rural China: A cross-sectional study.

J Affect Disord 2021 Jun 2;288:99-106. Epub 2021 Apr 2.

Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Department of Social Medicine and Health Administration, School of Public Health, Tianjin Medical University, Tianjin, China. Electronic address:

Background: With an aging population, late-life depression has been a major health problem in rural China. This study aims to explore the gender-specific prevalence of geriatric depression in rural Tianjin, its influencing factors, and to provide a scientific basis for the prevention and intervention of depression in the elderly.

Methods: A cross-sectional study of 4,933 elderly individuals in rural Tianjin was conducted using the cluster sampling method. The independent samples t-test and chi-squared test were used to assess differences in participants' characteristics by depressive symptoms, while multiple linear regressions and multiple logistic regressions were used to analyze the potential influencing factors of depression.

Results: The prevalence of geriatric depression was found to be 12.2% in the study participants (9.5% in men and 14.5% in women). Gender, education, household income, employment, living alone, social activities, physical exercise and chronic diseases were associated with depression (P<0.05). In addition to the above factors, sleep duration was also related with scores on self-rating depression scale (P<0.05).

Limitations: The study used a cross-sectional approach, so causation cannot be concluded.

Conclusions: Late-life depression is a serious mental health issue in rural China, highlighting the importance of appropriate diagnosis and treatment as a priority to improve the quality of mental health.
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http://dx.doi.org/10.1016/j.jad.2021.03.078DOI Listing
June 2021

Myocardial strain features by 2D-STE during the course of fulminant myocarditis: Correlation with characteristics by CMR and clinical implications.

Medicine (Baltimore) 2021 Apr;100(15):e25050

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.

Abstract: Myocardial strain analysis by 2D speckle tracking echocardiography could determine the left ventricular function. Our purpose is to investigate the global longitudinal strain (GLS) changes during the course of fulminant myocarditis (FM) and evaluate their correlation with cardiac magnetic resonance (CMR).Patients with clinical diagnosis of FM from June 30, 2017 to June 30, 2019 were screened prospectively. 18 survived patients (mean age 34 ± 18 years) who had two scans of transthoracic echocardiography and underwent CMR were included.All patients had severely impaired left ventricular ejection fraction and GLS value at admission that improved significantly before discharge. The patients in the healed stage revealed elevated global native T1 and T2 relaxation time and extracellular volume fraction as well, which were 1408.3 ± 88.3ms, 46.56 ± 5.23ms, and 0.35 ± 0.09, respectively. GLS from the second transthoracic echocardiography in the healed stage correlated significantly with global native T1 relaxation time (r =-0.574, P = .013) and with extracellular volume fraction (r = -0.582, P = .011), but not global native T2 relaxation time (r = -0.31, P = .211) and not with late gadolinium enhancement mass (r = 0.084, P = .743). In comparison, GLS at admission were not correlated with CMR parameters of fibrosis and oedema in the healed stage.GLS by 2D-STE may emerge as a new tool to monitor inflammatory myocardial injuries during the course of FM. FM in the acute healed stage has the presence of both chronic fibrosis and oedema which are correlated with GLS, but GLS at admission can't predict the early recovery of myocardial inflammation.
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http://dx.doi.org/10.1097/MD.0000000000025050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052038PMC
April 2021

DNA-Encoded Libraries: Hydrazide as a Pluripotent Precursor for On-DNA Synthesis of Various Azole Derivatives.

Chemistry 2021 Jun 27;27(31):8214-8220. Epub 2021 Apr 27.

Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.

DNA-encoded combinatorial chemical library (DEL) technology, an approach that combines the power of genetics and chemistry, has emerged as an invaluable tool in drug discovery. Skeletal diversity plays a fundamental importance in DEL applications, and relies heavily on novel DNA-compatible chemical reactions. We report herein a phylogenic chemical transformation strategy using DNA-conjugated benzoyl hydrazine as a common versatile precursor in azole chemical expansion of DELs. DNA-compatible reactions deriving from the common benzoyl hydrazine precursor showed excellent functional group tolerance with exceptional efficiency in the synthesis of various azoles, including oxadiazoles, thiadiazoles, and triazoles, under mild reaction conditions. The phylogenic chemical transformation strategy provides DELs a facile way to expand into various unique chemical spaces with privileged scaffolds and pharmacophores.
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http://dx.doi.org/10.1002/chem.202100850DOI Listing
June 2021

Discovery of a Potent and Selective FLT3 Inhibitor ()--(5-((5-Fluoro-2-oxoindolin-3-ylidene)methyl)-4-methyl-1-pyrrol-3-yl)-3-(pyrrolidin-1-yl)propanamide with Improved Drug-like Properties and Superior Efficacy in FLT3-ITD-Positive Acute Myeloid Leukemia.

J Med Chem 2021 04 2;64(8):4870-4890. Epub 2021 Apr 2.

Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, P.R. China.

Overcoming the FLT3-ITD mutant has been a promising drug design strategy for treating acute myeloid leukemia (AML). Herein, we discovered a novel FLT3 inhibitor , which displayed potent inhibitory activity against the FLT3-ITD mutant (IC = 0.8 nM) and achieved good selectivity over c-KIT kinase (over 500-fold). Compound selectively inhibited the proliferation of FLT3-ITD-positive AML cell lines MV4-11 (IC = 23.5 nM) and MOLM-13 (IC = 35.5 nM) and exhibited potent inhibitory effects against associated acquired resistance mutations. In cellular mechanism studies, compound strongly inhibited FLT3-mediated signaling pathways and induced apoptosis by arresting the cell cycle in the sub-G1 phase. In in vivo studies, compound demonstrated a good bioavailability (73.6%) and significantly suppressed tumor growth in MV4-11 (10 mg/kg, TGI 93.4%) and MOLM-13 (20 mg/kg, TGI 98.0%) xenograft models without exhibiting obvious toxicity. These results suggested that compound may be a promising drug candidate for treating FLT3-ITD-positive AML.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02247DOI Listing
April 2021

Perovskite Light-Emitting Diodes with External Quantum Efficiency Exceeding 22% via Small-Molecule Passivation.

Adv Mater 2021 May 1;33(18):e2007169. Epub 2021 Apr 1.

Key Laboratory of Semiconductor Materials Science, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

Perovskite light-emitting diodes (PeLEDs) are considered as particularly attractive candidates for high-quality lighting and displays, due to possessing the features of wide gamut and real color expression. However, most PeLEDs are made from polycrystalline perovskite films that contain a high concentration of defects, including point and extended imperfections. Reducing and mitigating non-radiative recombination defects in perovskite materials are still crucial prerequisites for achieving high performance in light-emitting applications. Here, ethoxylated trimethylolpropane triacrylate (ETPTA) is introduced as a functional additive dissolved in antisolvent to passivate surface and bulk defects during the spinning process. The ETPTA can effectively decrease the charge trapping states by passivation and/or suppression of defects. Eventually, the perovskite films that are sufficiently passivated by ETPTA make the devices achieve a maximum external quantum efficiency (EQE) of 22.49%. To our knowledge, these are the most efficient green PeLEDs up to now. In addition, a threefold increase in the T operational time of the devices was observed, compared to control samples. These findings provide a simple and effective strategy to make highly efficient perovskite polycrystalline films and their optoelectronics devices.
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http://dx.doi.org/10.1002/adma.202007169DOI Listing
May 2021

Advances in single-molecule fluorescent nanosensors.

Wiley Interdiscip Rev Nanomed Nanobiotechnol 2021 Mar 28:e1716. Epub 2021 Mar 28.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, China.

Single-molecule detection represents the ultimate sensitivity in measurement science with the characteristics of simplicity, rapidity, low sample consumption, and high signal-to-noise ratio and has attracted considerable attentions in biosensor development. In recent years, a variety of functional nanomaterials with unique chemical, optical, mechanical, and electronic features have been synthesized. The integration of single-molecule detection with functional nanomaterials enables the construction of novel single-molecule fluorescent nanosensors with excellent performance. Herein, we review the advance in single-molecule fluorescent nanosensors constructed by novel nanomaterials including quantum dots, gold nanoparticles, upconversion nanoparticles, fluorescent conjugated polymer nanoparticles, nanosheets, and magnetic nanoparticles in the past decade (2011-2020), and discuss the strategies, features, and applications of single-molecule fluorescent nanosensors in the detection of microRNAs, DNAs, enzymes, proteins, viruses, and live cells. Moreover, we highlight the future direction and challenges in this area. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle-Based Sensing Diagnostic Tools > Diagnostic Nanodevices.
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http://dx.doi.org/10.1002/wnan.1716DOI Listing
March 2021

Power-law graphs with small diameter: Framework, structural properties, and average trapping time.

Authors:
Fei Ma Ping Wang

Phys Rev E 2021 Feb;103(2-1):022318

National Engineering Research Center for Software Engineering, Peking University, Beijing 100871, China.

Here, we propose a simple algorithmic framework for creating power-law graphs with small diameters and then study structural properties, for instance, average degree, on graphs built. The results show that our graphs have not only some commonly seen properties including scale-free feature, small-world property, and disassortative structure, but also many rarely found characteristics, such as the density feature due to power-law exponents equal to 2 and the diameter equivalent to 2, compared to most previous scale-free models. In addition, we also consider the trapping problem on the proposed graphs and then find that they have more optimal trapping efficiency by means of their own average trapping times achieving the theoretical lower bound, a phenomenon that is seldom observed in existing scale-free models. We conduct extensive simulations, and the results show that empirical simulations are consistent with theoretical analysis.
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http://dx.doi.org/10.1103/PhysRevE.103.022318DOI Listing
February 2021

Highly tunable electronic structure and linear dichroism in 90° twisted α-phosphorus carbide bilayer: a first-principles calculation.

Phys Chem Chem Phys 2021 Mar 18;23(12):7080-7087. Epub 2021 Mar 18.

State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.

α-Phosphorus carbide (α-PC) shares a similar puckered structure with black phosphorus and has a high carrier mobility, showing great application potential in the future nano-electronic devices. Based on first-principles calculations, we reveal that an interlayer twist angle of 90° results in a symmetric band dispersion and spatial separation of electronic states in the α-PC bilayer, leading to isotropic electrical transport. Nevertheless, the anisotropic electronic states can be rebooted by introducing an out-of-plane electrostatic potential or an in-plane deformation potential, both of which can break the energy degeneracy and continuously modulate the bandgap of the 90° twisted bilayer. This highly tunable band structure can also induce a directionally exchangeable optical linear dichroism by flipping the voltage sign or changing the strain mode. These results indicate that the combination of the interlayer twist and gating/strain technique provides great flexibility to control the anisotropic behaviors in 2D puckered materials.
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http://dx.doi.org/10.1039/d0cp06619bDOI Listing
March 2021

Interactions Between Handgrip Strength and Serum Folate and Homocysteine Levels on Cognitive Function in the Elderly Chinese Population.

J Alzheimers Dis 2021 ;80(4):1503-1513

Department of Nutrition & Food Science, School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Handgrip strength (HGS) and serum folate and homocysteine (Hcy) levels were associated with cognitive function. However, little was known whether there were interactions between HGS and serum folate and Hcy levels on cognitive function.

Objective: To examine the interactions between HGS and serum folate and Hcy levels on cognitive function.

Methods: This study analyzed the baseline data of the Tianjin Elderly Nutrition and Cognition Cohort study. All participants aged ≥60 years were potential eligible. HGS was measured using a grip strength dynamometer. Serum folate and Hcy levels were assayed using standard laboratory protocol. A Mini-Mental State Examination was used to assess cognitive function. Linear regressions were employed to examine the interactions between HGS and serum folate and Hcy levels on cognitive function.

Results: 4,484 participants were included in this study. There were interactions between HGS and serum folate and Hcy levels on cognitive function. Furthermore, subjects with strong HGS and sufficient folate level had the best cognitive function (β= 2.018), sequentially followed by those with strong HGS and insufficient folate level (β= 1.698) and with poor HGS and sufficient folate level (β= 0.873). Similarly, cognitive function was ranked in the descending order of subjects with strong HGS and normal Hcy level (β= 1.971), strong HGS and high Hcy level (β= 1.467), and poor HGS and normal Hcy level (β= 0.657).

Conclusion: There were interactions between HGS and serum folate and Hcy levels on cognitive function. However, the temporal associations cannot be examined in a cross-sectional study. Further cohort study should be conducted to confirm these associations in the future.
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http://dx.doi.org/10.3233/JAD-201537DOI Listing
January 2021

Anlotinib has good efficacy and low toxicity: a phase II study of anlotinib in pre-treated HER-2 negative metastatic breast cancer.

Cancer Biol Med 2021 Mar 12. Epub 2021 Mar 12.

Department of VIP Medical Services, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Objective: Anlotinib is a novel tyrosine kinase inhibitor blocking angiogenesis. This study was performed to assess the efficacy and safety of anlotinib in patients with metastatic breast cancer.

Methods: Patients with HER2-negative breast cancer, who were pre-treated with anthracycline or taxanes in a neoadjuvant, adjuvant, or metastatic setting, and had treatment failure after at least one prior chemotherapy regimen in the metastatic setting were enrolled. Anlotinib was administered at 12 mg daily for 14 days in a 21-day cycle until disease progression or unacceptable toxicity occurred. Simultaneously, 5-10 mL of venous blood was collected to perform circulating tumor DNA (ctDNA) testing every 2 treatment cycles. The primary endpoint was the objective response rate (ORR). Secondary endpoints included the disease control rate (DCR), progression-free survival (PFS), overall survival, safety, and biomarkers.

Results: Twenty-six eligible patients were enrolled, with a median age of 56 (30-75) years. The median follow-up time was 10.5 months. The ORR was 15.4%, the DCR was 80.8%, and the median PFS was 5.22 months (95% confidence interval 2.86-6.24). Fourteen (53.8%) patients survived for more than 10 months. The changes in the detectable ctDNA variant allele frequency were consistent with the tumor response. The most common treatment-related adverse events were hypertension (57.7%), thyroidstimulating hormone elevation (34.6%), and hand-foot syndrome (23.1%).

Conclusions: Anlotinib showed objective efficacy with tolerable toxicity in heavily pre-treated, metastatic HER2-negative breast cancer. The dynamic changes in the ctDNA variant allele fraction may be predictive of the tumor response.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0463DOI Listing
March 2021

Short-term outcomes of laparoscopic versus open proximal gastrectomy with double-tract reconstruction for Siewert type II and III adenocarcinoma of the esophagogastric junction: a retrospective observational study of consecutive patients.

Ann Transl Med 2021 Feb;9(4):352

Department of General Surgery, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, China.

Background: To investigate the safety and merits of laparoscopic proximal gastrectomy with double-tract reconstruction (LPG-DT) for Siewert type II and III adenocarcinoma of the esophagogastric junction (AEG).

Methods: Retrospective analysis of the clinical data of 100 consecutive patients with Siewert II and III AEG treated at the Affiliated Tumor Hospital of Zhengzhou University from October 2010 to October 2019 was performed. Out of these patients, 69 underwent open proximal gastrectomy with double-tract reconstruction (OPG-DT), while 31 underwent LPG-DT. The clinicopathological characteristics, perioperative data, and short-term outcomes of the two groups were compared. A P value <0.05 was considered statistically significant.

Results: Males accounted for 87% of all patients. Lymph nodes (LNs) count, time to first meal, postoperative length of stay, and postoperative complications were similar between the OPG-DT and LPG-DT group. flatus time was significantly shorter in the LPG-DT group (P<0.05), while the duration of operation was significantly shorter in the the OPG-DT group (P<0.001). Furthermore, the LPG-DT group has less blood loss, shorter flatus time, and lower postoperative-day-5 white blood cell (WBC) count and C-reactive protein (CRP) levels (P<0.05).

Conclusions: Although LPG-DT took longer to perform, its advantages of reduced blood loss and less surgical stress reflected on inflammatory markers supports an acceptable surgical option for Siewert II and III AEG.
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http://dx.doi.org/10.21037/atm-21-130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944316PMC
February 2021