Publications by authors named "Fei Jin"

107 Publications

Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response to .

Int J Mol Sci 2021 Sep 2;22(17). Epub 2021 Sep 2.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China.

Broomcorn millet ( L.) affected by smut (caused by the pathogen ) has reduced production yields and quality. Determining the tolerance of broomcorn millet varieties is essential for smut control. This study focuses on the differences in the phenotypes, physiological characteristics, and transcriptomes of resistant and susceptible broomcorn millet varieties under stress. In diseased broomcorn millet, the plant height and stem diameter were reduced, while the number of nodes increased. After infection, the activities of superoxide dismutase and peroxidase decreased, and malondialdehyde and relative chlorophyll content (SPAD) decreased. Transcriptome analysis showed 514 and 5452 differentially expressed genes (DEGs) in the resistant and susceptible varieties, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs showed that pathways related to plant disease resistance, such as phenylpropanoid biosynthesis, plant-pathogen interaction, and plant hormone signal transduction, were significantly enriched. In addition, the transcriptome changes of cluster leaves and normal leaves in diseased broomcorn millet were analysed. Gene ontology and KEGG enrichment analyses indicated that photosynthesis played an important role in both varieties. These findings lay a foundation for future research on the molecular mechanism of the interaction between broomcorn millet and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22179542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430493PMC
September 2021

A promising crop for cadmium-contamination remediation: Broomcorn millet.

Ecotoxicol Environ Saf 2021 Aug 19;224:112669. Epub 2021 Aug 19.

College of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, PR China. Electronic address:

Cadmium (Cd) pollution highly threatens food security and human health, and phytoremediation with Cd-tolerant plants is a cost-effective in situ method for remediation of Cd contamination. Broomcorn millet is known for its strong abiotic stress resistance and can be used as a pioneer crop in both marginal regions and newly reclaimed land. To evaluate their potential in remediation of Cd contamination, a total of 288 broomcorn millet core collections were investigated under hydroponic conditions to compare their capabilities in Cd tolerance, translocation, and accumulation. The core collections varied considerably in their growth parameters, Cd concentration, Cd translocation factor, Cd bioaccumulation factor, and Cd accumulation under Cd stress. According to the Cd tolerance index (TI) values, 160 varieties were Cd tolerant. The Cd TI was significantly positively correlated with Cd accumulation, and the shoot Cd concentrations of five Cd-tolerant varieties were more than 100 mgkg, the threshold for being Cd hyperaccumulators. Moreover, the concentrations of essential metal elements were significantly decreased in shoots, and Cd concentration had a significantly positive relationship with magnesium (Mg) and zinc (Zn) concentrations in roots under Cd stress. These results demonstrate that broomcorn millet shows considerable tolerance to Cd stress and great differences in Cd accumulation abilities among varieties. Accordingly, broomcorn millet is a promising plant species for Cd bioremediation, with valuable varieties that have been identified for further study on Cd tolerance mechanisms and the remediation of Cd contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112669DOI Listing
August 2021

Novel Pyrazole-4-acetohydrazide Derivatives Potentially Targeting Fungal Succinate Dehydrogenase: Design, Synthesis, Three-Dimensional Quantitative Structure-Activity Relationship, and Molecular Docking.

J Agric Food Chem 2021 Aug 12;69(33):9557-9570. Epub 2021 Aug 12.

Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.

Succinate dehydrogenase inhibitors (SDHIs) have emerged in fungicide markets as one of the fastest-growing categories that are widely applied in agricultural production for crop protection. Currently, the structural modification focusing on the flexible amide link of SDHI molecules is being gradually identified as one of the innovative strategies for developing novel highly efficient and broad-spectrum fungicides. Based on the above structural features, a series of pyrazole-4-acetohydrazide derivatives potentially targeting fungal SDH were constructed and evaluated for their antifungal effects against , and . Strikingly, the in vitro EC values of constructed pyrazole-4-acetohydrazides against , against and against were, respectively, determined as 0.27, 1.94, and 1.93 μg/mL, which were obviously superior to that of boscalid against (0.94 μg/mL), fluopyram against (9.37 μg/mL), and (1.94 μg/mL). Concurrently, the effects of the substituent steric, electrostatic, hydrophobic, and hydrogen-bond fields on structure-activity relationships were elaborated by the reliable comparative molecular field analysis and comparative molecular similarity index analysis models. Subsequently, the practical value of pyrazole-4-acetohydrazide derivative as a potential SDHI was ascertained by the relative surveys on the in vivo anti- preventative efficacy, inhibitory effects against fungal SDH, and molecular docking studies. The present results provide an indispensable complement for the structural optimization of antifungal leads potentially targeting SDH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.1c03399DOI Listing
August 2021

Expedient discovery for novel antifungal leads: 1,3,4-Oxadiazole derivatives bearing a quinazolin-4(3H)-one fragment.

Bioorg Med Chem 2021 Sep 26;45:116330. Epub 2021 Jul 26.

Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.

Developing novel fungicide candidates are intensively promoted by the rapid emergences of resistant fungi that outbreak on agricultural production. Aiming to discovery novel antifungal leads, a series of 1,3,4-oxadiazole derivatives bearing a quinazolin-4(3H)-one fragment were constructed for evaluating their inhibition effects against phytopathogenic fungi in vitro and in vivo. Systematically structural optimizations generated the bioactive molecule I that was identified as a promising inhibitor against Rhizoctonia solani with the in vivo preventative effect of 58.63% at 200 μg/mL. The observations that were captured by scanning electron microscopy and transmission electron microscopy demonstrated that the bioactive molecule I could induce the sprawling growth of hyphae, the local shrinkage and rupture on hyphal surfaces, the extreme swelling of vacuoles, the striking distortions on cell walls, and the reduction of mitochondria numbers. The above results provided an indispensable complement for the discovery of antifungal lead bearing a quinazolin-4(3H)-one and 1,3,4-oxadiazole fragment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmc.2021.116330DOI Listing
September 2021

Power Generation from Moisture Fluctuations Using Polyvinyl Alcohol-Wrapped Dopamine/Polyvinylidene Difluoride Nanofibers.

Small 2021 Sep 27;17(36):e2102550. Epub 2021 Jul 27.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, P. R. China.

Despite the boom in the water-triggered electric power generation technologies, few attempts have been made with a broader horizonyielding the electricity from sweat, which is of great value for low-power-consumption wearable electronics. Here, an electromechanical coupling and humidity-actuated two-in-one humidity actuator-driven piezoelectric generator (HAPG) are reported, that can yield continuous electric power from fluctuations in the ambient humidity. It is composed of polyvinyl alcohol (PVA)-wrapped highly aligned dopamine (DA)/polyvinylidene fluoride (PVDF) shell/core nanofibers ([email protected]/PVDF NFs). As-received [email protected]/PVDF NFs can exchange water with the ambient humidity to perform expansion and contraction and convert them into electric power. An all-fiber-based portable HAPG is fabricated and tested on human palm skin. The devices show high sensitivity and accuracy for converting the mental sweating-derived continuous moisture fluctuations into electric power. This electric power can be stored in capacitors, which is expected to power micro- and nano-electronic devices or be used in electrotherapy such as electrical stimulation to promote wound healing. Beyond this, the obtained voltage profiles exhibit unique features that can reflect the typical sweat damping oscillation curve features.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102550DOI Listing
September 2021

[Effect of muscle relaxants on the prognosis of neonates with congenital esophageal atresia-tracheoesophageal fistula after surgery].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Jul;23(7):735-738

Neonatal Center, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.

Objective: To summarize the experience in the application of muscle relaxants in the perioperative period in neonates with congenital esophageal atresia-tracheoesophageal fistula (EA-TEF).

Methods: A retrospective analysis was performed on the medical data of 58 previously untreated neonates with EA-TEF who were treated in the Neonatal Center of Beijing Children's Hospital, Capital Medical University from 2017 to 2019. The incidence rate of anastomotic leak was compared between the neonates receiving muscle relaxants for different durations after surgery (≤ 5 days and > 5 days). The correlation between the duration of postoperative use of muscle relaxants and the duration of mechanical ventilation was evaluated.

Results: Among the 58 neonates with EA-TEF, 44 underwent surgery, among whom 35 with type III EA-TEF underwent thoracoscopic surgery. Among these 35 neonates, 30 (86%) received muscle relaxants after surgery, with a median duration of 4.75 days, and 6 (18%) experienced anastomotic leak. There was no significant difference in the incidence rate of anastomosis leak between the ≤ 5 days and > 5 days groups ( > 0.05). The duration of postoperative invasive mechanical ventilation was positively correlated with the duration of the use of muscle relaxants (=0.548, < 0.05).

Conclusions: Prolonged use of muscle relaxants after surgery cannot significantly reduce the incidence of anastomotic leak, but can prolong the duration of invasive mechanical ventilation in neonates with EA-TEF. Therefore, prolonged use of muscle relaxants is not recommended after surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292665PMC
July 2021

Dopamine/zinc oxide doped poly(-hydroxyethyl acrylamide)/agar dual network hydrogel with super self-healing, antibacterial and tissue adhesion functions designed for transdermal patch.

J Mater Chem B 2021 Jul;9(27):5492-5502

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China.

Dual network (DN) hydrogels with excellent mechanical strength and controllable component adjustment characteristics have a broad application range in the field of biomedicine. However, the tissue adhesion, skin affinity, self-healing, and antibacterial properties of DN hydrogels are inadequate for their application as skin patches. In this work, we prepared dopamine/zinc oxide (DOPA/ZnO) doped poly(N-hydroxyethyl acrylamide) (p(HEAA))/agar DN hydrogels and combined them to obtain a bilayer hydrogel (two-layer gel) with moisturizing properties. Upon incorporating 0.86 wt% of dopamine (DOPA), the resultant DOPA/p(HEAA))/agar DN hydrogel ([email protected]) exhibited high tensile strain (up to 1600%), excellent self-repair ability, and tissue adhesion. ZnO/p(HEAA))/agar DN hydrogel (ZnO NG) obtained by incorporating 2 w/v ZnO nanoparticles (ZnO NPs) achieved high tensile strength (1.2 MPa), good antibacterial ability, and low charge transfer resistance. Moreover, ZnO NG, which has a tight structure, was employed as a protective layer for the two-layer gel, which can effectively slow down the excessive evaporation of water to protect the [email protected] stability as a skin patch. Evidence showed that the two-layer hydrogel has water retention. Water retention still remains at over 50% after keeping the hydrogel in air for 3 days. These properties mean the two-layer gel based on the DOPA/ZnO doped DN hydrogels could be used as a transdermal patch for numerous applications in drug delivery, wearable devices, and electronic skin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1tb00822fDOI Listing
July 2021

Retrieval of an Intracardiac Cannula Fragment via Femoral Access in a Premature Infant.

Neonatology 2021 18;118(3):373-377. Epub 2021 Jun 18.

Neonatal Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

A preterm infant with birth weight 1,550 g had an intravenous foreign body between the external iliac vein and the common iliac vein, which accidentally happened during peripheral intravascular central catheter insertion by the Seldinger technique. The infant initially received conservative management and close monitoring. Antibiotics were administered 4 weeks to treat culture positive sepsis and meningitis. The infant was clinically stable till the cannula fragment migrated to the heart 34 days later. At that time, his weight was 2,200 g, and he was full fed. The cannula fragment was retrieved by emergency interventional radiology via the right femoral access, with no complications. The infant was discharged at 45 days of age and closely followed up post-discharge. He is currently 6 months old, with normal development. This is the first case of successful percutaneous retrieval of an intracardiac intravenous cannula fragment via femoral access in a premature infant in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000515464DOI Listing
August 2021

Stabilizing a three-center single-electron metal-metal bond in a fullerene cage.

Chem Sci 2021 Apr 2;12(20):6890-6895. Epub 2021 Apr 2.

Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Materials for Energy Conversion, Department of Materials Science and Engineering, Synergetic Innovation Center of Quantum Information & Quantum Physics, University of Science and Technology of China Hefei 230026 China

Trimetallic carbide clusterfullerenes (TCCFs) encapsulating a quinary MC cluster represent a special family of endohedral fullerenes with an open-shell electronic configuration. Herein, a novel TCCF based on a medium-sized rare earth metal, dysprosium (Dy), is synthesized for the first time. The molecular structure of [email protected] (7)-C determined by single crystal X-ray diffraction shows that the encapsulated DyC cluster adopts a bat ray configuration, in which the acetylide unit C is elevated above the Dy plane by ∼1.66 Å, while Dy-Dy distances are ∼3.4 Å. DFT computational analysis of the electronic structure reveals that the endohedral cluster has an unusual formal charge distribution of (Dy)(C)@C and features an unprecedented three-center single-electron Dy-Dy-Dy bond, which has never been reported for lanthanide compounds. Moreover, this electronic structure is different from that of the analogous [email protected] (7)-C with a (Sc)(C)@C charge distribution and no metal-metal bonding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1sc00965fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153215PMC
April 2021

Lethal, behavioral, growth and developmental toxicities of alkyl-PAHs and non-alkyl PAHs to early-life stage of brine shrimp, Artemia parthenogenetica.

Ecotoxicol Environ Saf 2021 Sep 18;220:112302. Epub 2021 May 18.

Key Laboratory for Ecological Environment in Coastal Areas, National Marine Environmental Monitoring Center, No. 42 Linghe Street, Dalian 116023, China; Marine Debris and Microplastic Research Center, Dalian 116023, China. Electronic address:

Alkyl-PAHs are the predominant form of PAHs in crude oils which are supposed to demonstrate different toxicities compared to non-alkyl PAHs. Little information is available about the toxicity of alkyl-PAHs on marine Artemia. This study addressed and compared the lethal, behavioral, growth and developmental toxicities of three alkyl-PAHs, namely 3-methyl phenanthrene (3-mPhe), retene (Ret) and 2-methyl anthracene (2-mAnt), to their non-alkyl forms, phenanthrene (Phe) and anthracene (Ant) using Artemia parthenogenetica (nauplii, <24 h) as test organism following a 48 h and a 7 d of exposure, respectively. Benzo-a-pyrene (Bap) was selected as a reference toxicant for the comparison with the above alkyl-PAHs and non-alkyl PAHs. Results showed that for all tested endpoints, A. parthenogenetica nauplii had the highest sensitivity to Bap while Ant had no significant effect on nauplii survival or development within given concentrations. Considering the aqueous freely dissolved PAH concentrations, the 48 h-LC (survival), 48 h-EC (immobility) and 7 d-LC (survival) of Bap were calculated as 0.321, 0.285 and 0.027 μg/L, respectively, which were twofold to fivefold lower than those of Phe, 3-mPhe, Ret, Ant and 2-mAnt. A higher acute toxicity of alkyl-PAHs (3-mPhe and 2-mAnt) than their non-alkyl forms (Phe and Ant) was observed. Not limited to Phe, the common non-polar narcotic mode of action was also observed for Bap, 3-mPhe, Ret and 2-mAnt, which was evident by the inhibited mobility of nauplii. The decreased body lengths were found for all PAH treatments compared to the solvent control, whereas instar retardations were only found in nauplii exposed to Bap, Phe and Ret. Our findings emphasized the sensitivity differences of A. parthenogenetica nauplii to selected alkyl PAHs and non-alkyl PAHs and confirmed the application of lethal, behavioral and growth indicators in the toxicity evaluation of selected PAHs other than Ant. However, the distinct toxicities of these PAHs suggested other toxic modes of action may play more important roles apart from narcotic mode of action and need to be elucidated in future studies. In addition, a strong correlation between the body length and the instar of A. parthenogenetica nauplii was observed for each PAH exposure, suggesting that body length can be representative for both growth and developmental indicators during biological monitoring of PAH pollution in marine environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112302DOI Listing
September 2021

Capturing the Missing Carbon Cage Isomer of C via Mutual Stabilization of a Triangular Monometallic Cyanide Cluster.

J Am Chem Soc 2021 Jun 19;143(21):8078-8085. Epub 2021 May 19.

Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Materials for Energy Conversion, Anhui Laboratory of Advanced Photon Science and Technology, Department of Materials Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Monometallic cyanide clusterfullerenes (CYCFs) represent a unique branch of endohedral clusterfullerenes with merely one metal atom encapsulated, offering a model system for elucidating structure-property correlation, while up to now only C and C cages have been isolated for the pristine CYCFs. C is one of the most abundant fullerenes and has 24 isomers obeying the isolated pentagon rule (IPR), among which 14 isomers have been already isolated, whereas the (17)-C isomer has lower relative energy than several isolated isomers but never been found for empty and endohedral fullerenes. Herein, four novel C-based pristine CYCFs with variable encapsulated metals and isomeric cages, including [email protected](13)-C (M = Y, Dy, Tb) and [email protected](17)-C, have been synthesized and isolated, fulfilling the first identification of the missing (17)-C isomer, which can be interconverted from the (13)-C isomer through two steps of Stone-Wales transformation. The molecular structures of these four C-based CYCFs are determined unambiguously by single-crystal X-ray diffraction. Surprisingly, although the ionic radii of Y, Dy, and Tb differ slightly by only 0.01 Å, such a subtle difference leads to an obvious change in the metal-cage interactions, as inferred from the distance between the metal atom and the nearest hexagon center of the (13)-C cage. On the other hand, upon altering the isomeric cage from [email protected](13)-C to [email protected](17)-C, the Dy-cage distance changes as well, indicating the interplay between the encapsulated DyCN cluster and the outer cage. Therefore, we demonstrate that the metal-cage interactions within CYCFs can be steered via both internal and external routes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c02428DOI Listing
June 2021

Population pharmacokinetics-pharmacodynamics of ceftazidime in neonates and young infants: Dosing optimization for neonatal sepsis.

Eur J Pharm Sci 2021 Aug 2;163:105868. Epub 2021 May 2.

Department of Clinical Pharmacy, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China; Department of Clinical Pharmacy, Clinical Trial Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Engineering and Technology Research Center for Pediatric Drug Development, Shandong Medicine and Health Key Laboratory of Clinical Pharmacy, Jinan 250014, China. Electronic address:

Ceftazidime is a third-generation cephalosporin with high activity against many pathogens. But the ambiguity and diversity of the dosing regimens in neonates and young infants impair access to effective treatment. Thus, we conducted a population pharmacokinetic study of ceftazidime in this vulnerable population and recommended a model-based dosage regimen to optimize sepsis therapy. Totally 146 neonates and young infants (gestational age (GA): 36-43.4 weeks, postnatal age (PNA): 1-81 days, current weight (CW): 900-4500 g) were enrolled based on inclusion and exclusion criteria. Ceftazidime bloods samples (203) were obtained using the opportunistic sampling strategy and determined by the high-performance liquid chromatography. The population pharmacokinetic-pharmacodynamic analysis was conducted by nonlinear mixed effects model (NONMEM). A one-compartment model with first-order elimination best described the pharmacokinetic data. Covariate analysis showed the significance of GA, PNA, and CW on developmental pharmacokinetics. Monte Carlo simulation was performed based on above covariates and minimum inhibitory concentration (MIC). In the newborns with PNA ≤ 3 days (MIC=8 mg/L), the dose regimen was 25 mg/kg twice daily (BID). For the newborns with PNA > 3 days (MIC=16 mg/L), the optimal dose was 30 mg/kg three times daily (TID) for those with GA ≤ 37 weeks and 40 mg/kg TID for those with GA > 37 weeks. Overall, on the basis of the developmental population pharmacokinetic-pharmacodynamic analysis covering the whole range of neonates and young infants, the evidence-based ceftazidime dosage regimens were proposed to optimize neonatal early-onset and late-onset sepsis therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejps.2021.105868DOI Listing
August 2021

The structure of MgtE in the absence of magnesium provides new insights into channel gating.

PLoS Biol 2021 04 27;19(4):e3001231. Epub 2021 Apr 27.

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Shanghai Key Laboratory of Bioactive Small Molecules, Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai, China.

MgtE is a Mg2+ channel conserved in organisms ranging from prokaryotes to eukaryotes, including humans, and plays an important role in Mg2+ homeostasis. The previously determined MgtE structures in the Mg2+-bound, closed-state, and structure-based functional analyses of MgtE revealed that the binding of Mg2+ ions to the MgtE cytoplasmic domain induces channel inactivation to maintain Mg2+ homeostasis. There are no structures of the transmembrane (TM) domain for MgtE in Mg2+-free conditions, and the pore-opening mechanism has thus remained unclear. Here, we determined the cryo-electron microscopy (cryo-EM) structure of the MgtE-Fab complex in the absence of Mg2+ ions. The Mg2+-free MgtE TM domain structure and its comparison with the Mg2+-bound, closed-state structure, together with functional analyses, showed the Mg2+-dependent pore opening of MgtE on the cytoplasmic side and revealed the kink motions of the TM2 and TM5 helices at the glycine residues, which are important for channel activity. Overall, our work provides structure-based mechanistic insights into the channel gating of MgtE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pbio.3001231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104411PMC
April 2021

A Highly Strained Phase in PbZrTiO Films with Enhanced Ferroelectric Properties.

Adv Sci (Weinh) 2021 Apr 18;8(8):2003582. Epub 2021 Feb 18.

Department of Physics Southern University of Science and Technology Shenzhen Guangdong 518055 China.

Although epitaxial strain imparted by lattice mismatch between a film and the underlying substrate has led to distinct structures and emergent functionalities, the discrete lattice parameters of limited substrates, combined with strain relaxations driven by film thickness, result in severe obstructions to subtly regulate electro-elastic coupling properties in perovskite ferroelectric films. Here a practical and universal method to achieve highly strained phases with large tetragonal distortions in Pb-based ferroelectric films through synergetic effects of moderately (≈1.0%) misfit strains and laser fluences during pulsed laser deposition process is demonstrated. The phase possesses unexpectedly large Poisson's ratio and negative thermal expansion, and concomitant enhancements of spontaneous polarization (≈100 µC cm) and Curie temperature (≈800 °C), 40% and 75% larger than that of bulk counterparts, respectively. This strategy efficiently circumvents the long-standing issue of limited numbers of discrete substrates and enables continuous regulations of exploitable lattice states in functional oxide films with tightly elastic coupled performances beyond their present levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202003582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061395PMC
April 2021

18F-FDG PET/CT in Infective Endocarditis on Papillary Muscles: A Case Report.

Clin Nucl Med 2021 06;46(6):501-504

From the Department of Molecular Imaging, Qingdao Central Hospital, Qingdao University, Qingdao, Shandong, People's Republic of China.

Abstract: Infective endocarditis is a serious, potentially life-threatening condition. The valve leaflets and annulus are the most commonly affected sites, but involvement of the papillary muscles is extremely rare. We present the case of a 69-year-old woman who had infective endocarditis only involving the papillary muscle on 18F-FDG PET/CT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/RLU.0000000000003606DOI Listing
June 2021

Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins.

Commun Biol 2021 03 19;4(1):366. Epub 2021 Mar 19.

State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai, China.

GFP fusion-based fluorescence-detection size-exclusion chromatography (FSEC) has been widely employed for membrane protein expression screening. However, fused GFP itself may occasionally affect the expression and/or stability of the targeted membrane protein, leading to both false-positive and false-negative results in expression screening. Furthermore, GFP fusion technology is not well suited for some membrane proteins, depending on their membrane topology. Here, we developed an FSEC assay utilizing nanobody (Nb) technology, named FSEC-Nb, in which targeted membrane proteins are fused to a small peptide tag and recombinantly expressed. The whole-cell extracts are solubilized, mixed with anti-peptide Nb fused to GFP for FSEC analysis. FSEC-Nb enables the evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification but does not require direct GFP fusion to targeted proteins. Our results show FSEC-Nb as a powerful tool for expression screening of membrane proteins for structural and functional studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s42003-021-01891-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979870PMC
March 2021

Structural basis for the Mg recognition and regulation of the CorC Mg transporter.

Sci Adv 2021 Feb 10;7(7). Epub 2021 Feb 10.

State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, and Department of Physiology and Biophysics, School of Life Sciences, Fudan University, 2005 Songhu Road, Yangpu District, Shanghai 200438, China.

The CNNM/CorC family proteins are Mg transporters that are widely distributed in all domains of life. In bacteria, CorC has been implicated in the survival of pathogenic microorganisms. In humans, CNNM proteins are involved in various biological events, such as body absorption/reabsorption of Mg and genetic disorders. Here, we determined the crystal structure of the Mg-bound CorC TM domain dimer. Each protomer has a single Mg binding site with a fully dehydrated Mg ion. The residues at the Mg binding site are strictly conserved in both human CNNM2 and CNNM4, and many of these residues are associated with genetic diseases. Furthermore, we determined the structures of the CorC cytoplasmic region containing its regulatory ATP-binding domain. A combination of structural and functional analyses not only revealed the potential interface between the TM and cytoplasmic domains but also showed that ATP binding is important for the Mg export activity of CorC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abe6140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875539PMC
February 2021

Safety and immunogenicity of a novel oral hexavalent rotavirus vaccine:a phase I clinical trial.

Hum Vaccin Immunother 2021 07 5;17(7):2311-2318. Epub 2021 Feb 5.

Wuhan Institute of Biological Products Co., Ltd., Wuhan, People's Republic of China.

Rotavirus infections, prevalent in human populations, are caused mostly by group A viruses. Immunization against rotaviruses in infancy is currently the most effective and economical strategy to prevent rotavirus infection. This study evaluated the safety of a novel hexavalent rotavirus vaccine and analyzed its dose and immunogenicity. This randomized, double-blinded, placebo-controlled phase I clinical trial enrolled healthy adults, toddlers, and infants in Zhengding County, Hebei Province, northern China. 40 adults and 40 children were assigned in a 2:1:1 ratio to receive one vaccine dose, placebo 1, and placebo 2, respectively. 120 6-12 week old infants were assigned equivalently into 3 groups. The infants in each group were assigned in a 2:1:1 ratio to receive three doses of vaccine, placebo 1, and placebo 2, at a 28-day interval. Adverse events (AEs) until 28 days after each dose and serious adverse events (SAEs) until 6 months after the third dose were reported. Virus shedding until 14 days after each dose in infants was tested. Geometric mean concentrations (GMCs) and seroconversion rates were measured for anti-rotavirus IgA by using an enzyme-linked immunosorbent assay (ELISA). The solicited and unsolicited AE frequencies and laboratory indexes were similar among the treatment groups. No vaccine-related SAEs were reported. The average percentage of rotavirus vaccine shedding in the infant vaccine groups was 5.00%. The post-3rd dose anti-rotavirus IgA antibody geometric mean concentrations (GMC) and seroconversion rate were higher in the vaccine groups than in the placebo groups. The novel oral hexavalent rotavirus vaccine was generally well-tolerated in all adults, toddlers and infants, and the vaccine was immunogenic in infants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21645515.2020.1861874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189138PMC
July 2021

CaCoMnO: A Promising Perovskite Solid Solution for Solar Thermochemical Energy Storage.

ACS Appl Mater Interfaces 2021 Jan 11;13(3):3856-3866. Epub 2021 Jan 11.

Key Laboratory of Power Station Energy Transfer Conversion and System of MOE, School of Energy Power and Mechanical Engineering, North China Electric Power University, Beijing 102206, P. R. China.

The redox cycle of doped CaMnO has emerged as an attractive way for cost-effective thermochemical energy storage (TCES) at high temperatures in concentrating solar power. The role of dopants is mainly to improve the thermal stability of CaMnO at high temperatures and the overall TCES density of the material. Herein, Co-doped CaMnO (CaCoMnO, = 0-0.5) perovskites have been proposed as a promising candidate for TCES materials for the first time. The phase compositions, redox capacities, TCES densities, reaction rates, and redox chemistry of the samples have been explored via experimental analysis and theoretical calculations. The results demonstrate that CaCoMnO showed an enhanced redox capacity (1000 °C at O = 10 bar) without decomposition and provided the highest TCES density of ∼571 kJ kg reported so far. The effective Co doping tended to increase the valence states of B-site cations in perovskite and facilitate the diffusion of the lattice oxygen atoms into the surface-active oxygen sites. Furthermore, the high cooling rates deteriorated the microstructure of CaCoMnO particles and resulted in incomplete heat release, which is instructive to the design and operation of the TCES systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c18207DOI Listing
January 2021

Effect of Weekly Antibiotic Round on Antibiotic Use in the Neonatal Intensive Care Unit as Antibiotic Stewardship Strategy.

Front Pediatr 2020 15;8:604244. Epub 2020 Dec 15.

Neonatal Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Antibiotics are commonly used in the neonatal intensive care unit (NICU). The objective was to observe the effect of weekly antibiotic round in NICU (WARN) to the antibiotic use in NICU. A retrospective observational study was performed. Departmental-level diagnosis categories and the parameters of antibiotic usage in NICU for the period of 2016-2017 (Phase 1) and 2018-2019 (Phase 2) were collected. WARN in NICU was started since January 2018. A time series forecasting was used to predict the quarterly antibiotic use in Phase 2, based on data from Phase 1. The actual antibiotic use of each quarter in Phase 2 was compared with the predicted values. Totally 9297 neonates were included (4743 in Phase 1, 4488 in Phase 2). The composition of the disease spectrum between Phase 1 and Phase 2 was not different ( > 0.05). In Phase 1 and Phase 2, the overall antibiotic rate was 94.4 and 74.2%, the average accumulative defined daily dose per month was 199.00 ± 55.77 and 66.80 ± 45.64, the median antibiotic use density per month was 10.31 (9.00-13.27) and 2.48 (1.92-4.66), the median accumulative defined daily dose per case per month was 0.10 (0.09-0.13) and 0.03 (0.02-0.47), the number of patients who received any kind of antibiotic per 1000 hospital days per month was 103.45 (99.30-107.48) and 78.66 (74.62-82.77), rate of culture investigation before antibiotics was 64 to 92%, respectively, and all were better than the predicted values ( < 0.01). The implementation of periodical antibiotic rounds was effective in reducing the antibiotics use in the NICU.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2020.604244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769868PMC
December 2020

CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway.

Cell Discov 2020 Nov 17;6(1):85. Epub 2020 Nov 17.

State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.

Collaborator of ARF (CARF) regulates cell proliferative fate through both p53-dependent and -independent mechanisms. Recently, we reported a new function of CARF as a positive regulator of Wnt signaling. Despite these findings, the physiological function of CARF has not been well studied. Here, we generated CARF knockout mice and found that male CARF mice exhibited significantly impaired fertility and Sertoli-cell-only (SCO) syndrome phenotypes. Further studies revealed that loss of CARF in Sertoli cells led to decreased GDNF expression, which hindered spermatogonial stem cells (SSCs) self-renewal. Meanwhile, CARF loss in undifferentiated spermatogonia impaired their proliferation. These two mechanisms together led to SCO syndrome phenotypes, which could be functionally rescued by pharmacological or genetic reactivation of Wnt signaling. Finally, we identified CARF as a potential pathogenic mutation in an SCO patient. Overall, our findings reveal important roles of CARF in spermatogonial self-renewal and proliferation through the Wnt signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41421-020-00212-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674451PMC
November 2020

High-Performance Poly(vinylidene difluoride)/Dopamine Core/Shell Piezoelectric Nanofiber and Its Application for Biomedical Sensors.

Adv Mater 2021 Jan 4;33(3):e2006093. Epub 2020 Dec 4.

School of Chemical Engineering, Nanjing University of Science and Technology, #200 Xiaolingwei, Nanjing, 210094, P. R. China.

Fabrication of soft piezoelectric nanomaterials is essential for the development of wearable and implantable biomedical devices. However, a big challenge in this soft functional material development is to achieve a high piezoelectric property with long-term stability in a biological environment. Here, a one-step strategy for fabricating core/shell poly(vinylidene difluoride) (PVDF)/dopamine (DA) nanofibers (NFs) with a very high β-phase content and self-aligned polarization is reported. The self-assembled core/shell structure is believed essential for the formation and alignment of β-phase PVDF, where strong intermolecular interaction between the NH groups on DA and the CF groups on PVDF is responsible for aligning the PVDF chains and promoting β-phase nucleation. The as-received PVDF/DA NFs exhibit significantly enhanced piezoelectric performance and excellent stability and biocompatibility. An all-fiber-based soft sensor is fabricated and tested on human skin and in vivo in mice. The devices show a high sensitivity and accuracy for detecting weak physiological mechanical stimulation from diaphragm motions and blood pulsation. This sensing capability offers great diagnostic potential for the early assessment and prevention of cardiovascular diseases and respiratory disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202006093DOI Listing
January 2021

Elevated CXorf67 Expression in PFA Ependymomas Suppresses DNA Repair and Sensitizes to PARP Inhibitors.

Cancer Cell 2020 12 12;38(6):844-856.e7. Epub 2020 Nov 12.

State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address:

Ependymoma is the third most common pediatric tumor with posterior fossa group A (PFA) being its most aggressive subtype. Ependymomas are generally refractory to chemotherapies and thus lack any effective treatment. Here, we report that elevated expression of CXorf67 (chromosome X open reading frame 67), which frequently occurs in PFA ependymomas, suppresses homologous recombination (HR)-mediated DNA repair. Mechanistically, CXorf67 interacts with PALB2 and inhibits PALB2-BRCA2 interaction, thereby inhibiting HR repair. Concordantly, tumor cells with high CXorf67 expression levels show increased sensitivity to poly(ADP-ribose) polymerase (PARP) inhibitors, especially when combined with radiotherapy. Thus, our findings have revealed a role of CXorf67 in HR repair and suggest that combination of PARP inhibitors with radiotherapy could be an effective treatment option for PFA ependymomas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ccell.2020.10.009DOI Listing
December 2020

Application of Gastroscopy Based on Nano Carbon in the Remedy of Chronic Pulmonary Heart Disease Caused by Helicobacter Pylori.

J Nanosci Nanotechnol 2021 02;21(2):1127-1134

Department of Respiration, Xingtai Third Hospital, No. 108 Steel North Road, Qiaoxi District, Xingtai City, 054000, Hebei Province, China.

Chronic pulmonary heart disease is a common respiratory disease. Helicobacter pylori infection can lead to the occurrence of chronic pulmonary heart disease. However, most drugs for chronic pulmonary heart disease caused by helicobacter pylori are lack of tissue specificity. At the same time, due to the blocked blood circulation in the ischemic area, the distribution of drugs in the ischemic area is often not ideal. The gastroscope technology of nano carbon can make the drug release in the focus as much as possible, and can achieve the goal of targeted treatment. This paper mainly studies the application of the gastroscope technology based on nano carbon in the remedy of chronic pulmonary heart disease caused by helicobacter pylori. After 8 weeks of treatment, the indexes of right heart function in the two groups: TAPSE and RVMPI were better than before treatment, and the improvement degree in the remedy group was more obvious than that in the control group ( < 0.05). The improvement degree of E/A and PASP was not statistically significant, the difference between the two groups was not significant ( > 0.05). In addition, the gastroscope of nano carbon can also reduce UA and TG in blood. The overall response rate was 93.75% in the remedy group, which was higher than that in the control group ( < 0.05).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jnn.2021.18701DOI Listing
February 2021

Coupled effects of urbanization level and dam on microplastics in surface waters in a coastal watershed of Southeast China.

Mar Pollut Bull 2020 May 20;154:111089. Epub 2020 Mar 20.

Fujian Key Laboratory of Functional Marine Sensing Materials, Institute of Oceanography, Minjiang University, Fuzhou 350108, China; Department of Marine Chemistry, National Marine Environmental Monitoring Center, Dalian 116023, China. Electronic address:

Investigating the heterogeneous distribution pattern of plastic pollution in river systems is useful for better understanding the transport pathways of plastics from land to sea. Therefore, we analyzed samples from 17 sites to evaluate the distribution pattern of microplastics (MPs) in Minjiang River Watershed. The MP concentrations ranged between 0.12 and 2.72 n/L, with higher microplastic concentrations located in urban areas with greater population and gross domestic product (GDP) densities. MP concentrations were positively correlated with population density, GDP density, and built-up land use percentage, suggesting that increasing urbanization level will lead to greater plastic pollution. MP concentrations spanning the upstream and downstream sides of the SK Hydropower Dam showed decreasing trends, suggesting that MP settling would occur behind the dam and Shuikou Reservoir might serve as a sink for MPs. This study provides useful insight for revealing the effects of urbanization and dams on spatial distribution of riverine MPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.marpolbul.2020.111089DOI Listing
May 2020

Non-O1, Non-O139 (NOVC) Bacteremia: Case Report and Literature Review, 2015-2019.

Infect Drug Resist 2020 7;13:1009-1016. Epub 2020 Apr 7.

Department of Laboratory Medicine, Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, People's Republic of China.

Non-O1, non-O139 (NOVC) does not agglutinate with O1 and O139 antisera and can cause intestinal and extraintestinal infections in immunocompromised individuals. NOVC bacteremia has the highest mortality among NOVC infections, and the number of reports has increased in recent years. Nevertheless, some clinicians are poorly informed about this disease. Herein, we describe a documented case of NOVC bacteremia in a male patient with impaired liver function. Blood cultures revealed the presence of , but this strain showed self-coagulation on the serum agglutination test. To our knowledge, this phenomenon is unreported among cases of NOVC infections. This pathogen was finally confirmed as NOVC via PCR. Because the patient worked as a garbage transporter, he was likely infected after contact with contaminated water through a foot wound. The patient developed septic shock shortly after admission and ultimately died from the illness. This paper reviews 23 cases of NOVC bacteremia from 2015 to 2019. To improve the accuracy of identifying NOVC and analyze its virulence factors, relevant detection methods were reviewed and analyzed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S245806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156264PMC
April 2020

Successively Regioselective Electrosynthesis and Electron Transport Property of Stable Multiply Functionalized [60]Fullerene Derivatives.

Research (Wash D C) 2020 15;2020:2059190. Epub 2020 Feb 15.

Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Materials for Energy Conversion, and Department of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.

With the recent advance in chemical modification of fullerenes, electrosynthesis has demonstrated increasing importance in regioselective synthesis of novel fullerene derivatives. Herein, we report successively regioselective synthesis of stable tetra- and hexafunctionalized [60]fullerene derivatives. The cycloaddition reaction of the electrochemically generated dianions from [60]fulleroindolines with phthaloyl chloride regioselectively affords 1,2,4,17-functionalized [60]fullerene derivatives with two attached ketone groups and a unique addition pattern, where the heterocycle is rearranged to a [5,6]-junction and the carbocycle is fused to an adjacent [6,6]-junction. This addition pattern is in sharp contrast with that of the previously reported biscycloadducts, where both cycles are appended to [6,6]-junctions. The obtained tetrafunctionalized compounds can be successively manipulated to 1,2,3,4,9,10-functionalized [60]fullerene derivatives with an intriguing ""-shaped configuration a novel electrochemical protonation. Importantly, the stability of tetrafunctionalized [60]fullerene products allows them to be applied in planar perovskite solar cells as efficient electron transport layers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.34133/2020/2059190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044465PMC
February 2020

Effects of excessive impregnation, magnesium content, and pyrolysis temperature on MgO-coated watermelon rind biochar and its lead removal capacity.

Environ Res 2020 04 20;183:109152. Epub 2020 Jan 20.

Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, T6G 2E3, Canada.

MgO-coated watermelon rind biochar (MWRB) is a potentially highly-effective waste-derived material in environmental applications. This research aims to provide valuable insights into the optimization of the production of MWRB for superior environmental performance. It was found that the Mg content of the MWRB could be easily controlled by adjusting the Mg/feedstock mass ratio during excessive impregnation. The BET surface area was found to first increase and then decrease as the Mg content of the MWRB (produced at 600 °C) increased from 1.52% to 10.1%, with an optimal surface area of 293 m/g observed at 2.51%. Similarly, an optimum pyrolysis temperature of 600 °C was observed in the range of 400-800 °C for a maximum surface area of the MWRB at a fixed Mg/feedstock ratio of 0.48% (resulting in MWRBs with Mg contents of 1.89-2.51%). The Pb removal capacity of the MWRB (produced at 600 °C) increased with increasing Mg content, with a greatest Pb removal capacity of 558 mg/g found for the MWRB with the highest Mg content (10.1%), an improvement of 208% over the 181 mg/g Pb removal capacity of unmodified WRB produced at 600 °C. The Pb removal capacity of the MWRB (produced with 1.89-2.51% Mg) was also discovered to increase from 81.7 mg/g (at 400 °C) to 742 mg/g (at 700 °C), before dropping to 368 mg/g at 800 °C. These findings suggest that the MWRB can be more efficiently utilized in soil and water remediation by optimizing its synthesis conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2020.109152DOI Listing
April 2020

Immune response to different types of hepatitis B vaccine booster doses 2-32 years after the primary immunization schedule and its influencing factors.

Int J Infect Dis 2020 Apr 28;93:62-67. Epub 2020 Jan 28.

Hebei Province Center for Disease Control and Prevention, 97 Huai'an East Road, Yuhua District, Shijiazhuang 050021, Hebei, People's Republic of China. Electronic address:

Objective: To assess the immune effect of different types of hepatitis B vaccine (HepB) booster doses 2-32 years after primary immunization, explore the influencing factors, and offer guidance regarding the necessity and timing of boosters.

Methods: In total, 1163 participants who were born from 1986 to 2015, received the HepB full-course primary vaccination, were hepatitis B surface antigen (HBsAg) and hepatitis B core antibody (anti-HBc) negative, and had hepatitis B surface antibody (anti-HBs) <10 mIU/mL were enrolled. Individuals were randomly divided into two groups and received a booster dose of HepB. Venous blood samples were collected 30 days later and tested for anti-HBs.

Results: In total, 595 and 568 individuals received a single dose of HepB (CHO) and HepB (SC), respectively. Venous blood samples were obtained from 1079 vaccinees (CHO: 554, SC: 525). The seroconversion rates were 93.68% (519/554) and 86.67% (455/525) (p < 0.05), with geometric mean concentrations (GMCs) of 426.58 mIU/ml and 223.8 mIU/ml, respectively. This result indicated that BMI, smoking status, vaccine types of booster and prebooster anti-HBs concentration significantly influenced anti-HBs levels. Only BMI, prebooster anti-HBs concentrations and booster types were different between the anti-HBs positive and negative groups.

Conclusions: Participants boostered with HepB (CHO) had a relatively higher seroconversion rate than those boostered with HepB (SC). The high seroconversion rates in the two groups suggested that the subjects remained protected despite low circulating antibodies, so there is currently no urgent need for booster immunization. Factors including BMI ≥ 25 and prebooster anti-HBs concentration <2.5 mIU/mL, which contributed to lower responses to a booster dose, might indicate a greater risk of breakthrough infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijid.2020.01.047DOI Listing
April 2020

Green remediation of Cd and Hg contaminated soil using humic acid modified montmorillonite: Immobilization performance under accelerated ageing conditions.

J Hazard Mater 2020 04 2;387:122005. Epub 2020 Jan 2.

School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Solidification/Stabilization (S/S) is an effective way to immobilize toxic metals in contaminated soil. However, utilization of ordinary Portland cement (PC) in this process has raised environmental concerns owing to the high carbon footprint from PC manufacturing and the risk of toxic element leaching in the long term. Hence there is an urgent need to seek for "green" immobilization approaches with long-term stability. In this study, a clay-based material, humic acid modified montmorillonite (HA-Mont) was applied to a Cd and Hg contaminated soil. Field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (FESEM/EDS), N adsorption-desorption, Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses were performed to investigate the characteristics of this material. Compared to the soil without any treatment, dosage of 5 % HA-Mont could effectively reduce Cd and Hg concentrations by 94.1 % and 93.0 %, respectively and to below the regulatory limits in the TCLP (Toxicity Characteristic Leaching Procedure) leachates. Compared to the soil treated with virgin montmorillonite, HA modification resulted in the reduction of leachate concentrations of Cd and Hg by 69.5 % and 65.9 %, respectively. Long-term immobilization performance of the HA-Mont treatment was examined using a quantitative accelerated ageing method. In order to examine the ageing features, a novel method based on conditional probability was developed, and the reliability of HA-Mont immobilization was found to fit the Weibull model well, as the ageing rate of immobilization effect increased with time. After 120 years of ageing, reliability of both metals could still remain above 0.95. Cd concentration in TCLP leachates at 120 year could still remain below the regulatory limit (294 μg/L vs 1000 μg/L), while Hg concentration reached the regulatory limit of 200 μg/L in 96 year. This is the first attempt developing a green S/S method of Cd and Hg contaminated soil using HA-Mont and examining the long-term ageing characteristics of the stabilized soil using a probability-based approach.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2019.122005DOI Listing
April 2020
-->