Publications by authors named "Fei Fei Wang"

71 Publications

Three Resorcin[4]arene-Based Two-Dimensional Zn(II) Supramolecular Isomers Synthesized via a Structure-Directing Strategy for Knoevenagel Condensation.

Inorg Chem 2021 May 30;60(10):7329-7336. Epub 2021 Apr 30.

Key Laboratory of Polyoxometalate and Reticular Material Chemistry, Department of Chemistry, Northeast Normal University, Changchun 130024, People's Republic of China.

Herein, in the presence of three structure-directing agents (SDAs), a family of imidazole-functionalized resorcin[4]arene-based coordination polymers (CPs), [Zn(TIC4R)(HCOO)]·HCOO·0.5DMF·1.5HO (), [Zn(TIC4R)(CN)]·HCOO·DMF·2.5HO (), and [Zn(TIC4R)(HO)]·2HCOO·2HO (), were assembled under solvothermal conditions [TIC4R = tetra(imidazole) resorcin[4]arene]. exhibits a double-layer structure with rectangle windows, and and display monolayer structures. The layers of CPs and are slides with different offsets along the -axis. In addition, three CPs were used as catalysts to catalyze Knoevenagel condensations. Strikingly, all CPs exhibit remarkable catalytic performance for several substrates. To the best of our knowledge, this is the first time that a small organic acid as SDA was used in the syntheses of resorcin[4]arene-based supramolecular isomers.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00497DOI Listing
May 2021

A System for Phenotype Harmonization in the NHLBI Trans-Omics for Precision Medicine (TOPMed) Program.

Am J Epidemiol 2021 Apr 16. Epub 2021 Apr 16.

Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, Washington.

Genotype-phenotype association studies often combine phenotype data from multiple studies to increase power. Harmonization of the data usually requires substantial effort due to heterogeneity in phenotype definitions, study design, data collection procedures, and data set organization. Here we describe a centralized system for phenotype harmonization that includes input from phenotype domain and study experts, quality control, documentation, reproducible results, and data sharing mechanisms. This system was developed for the National Heart, Lung and Blood Institute's Trans-Omics for Precision Medicine program, which is generating genomic and other omics data for >80 studies with extensive phenotype data. To date, 63 phenotypes have been harmonized across thousands of participants from up to 17 studies per phenotype (participants recruited 1948-2012). We discuss challenges in this undertaking and how they were addressed. The harmonized phenotype data and associated documentation have been submitted to National Institutes of Health data repositories for controlled-access by the scientific community. We also provide materials to facilitate future harmonization efforts by the community, which include (1) the code used to generate the 63 harmonized phenotypes, enabling others to reproduce, modify or extend these harmonizations to additional studies; and (2) results of labeling thousands of phenotype variables with controlled vocabulary terms.
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http://dx.doi.org/10.1093/aje/kwab115DOI Listing
April 2021

Chromosome Xq23 is associated with lower atherogenic lipid concentrations and favorable cardiometabolic indices.

Nat Commun 2021 04 12;12(1):2182. Epub 2021 Apr 12.

Division of Cardiology, George Washington University School of Medicine and Healthcare Sciences, Washington, DC, USA.

Autosomal genetic analyses of blood lipids have yielded key insights for coronary heart disease (CHD). However, X chromosome genetic variation is understudied for blood lipids in large sample sizes. We now analyze genetic and blood lipid data in a high-coverage whole X chromosome sequencing study of 65,322 multi-ancestry participants and perform replication among 456,893 European participants. Common alleles on chromosome Xq23 are strongly associated with reduced total cholesterol, LDL cholesterol, and triglycerides (min P = 8.5 × 10), with similar effects for males and females. Chromosome Xq23 lipid-lowering alleles are associated with reduced odds for CHD among 42,545 cases and 591,247 controls (P = 1.7 × 10), and reduced odds for diabetes mellitus type 2 among 54,095 cases and 573,885 controls (P = 1.4 × 10). Although we observe an association with increased BMI, waist-to-hip ratio adjusted for BMI is reduced, bioimpedance analyses indicate increased gluteofemoral fat, and abdominal MRI analyses indicate reduced visceral adiposity. Co-localization analyses strongly correlate increased CHRDL1 gene expression, particularly in adipose tissue, with reduced concentrations of blood lipids.
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http://dx.doi.org/10.1038/s41467-021-22339-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042019PMC
April 2021

Functions of Dendritic Cells and Its Association with Intestinal Diseases.

Cells 2021 03 6;10(3). Epub 2021 Mar 6.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Dendritic cells (DCs), including conventional DCs (cDCs) and plasmacytoid DCs (pDCs), serve as the sentinel cells of the immune system and are responsible for presenting antigen information. Moreover, the role of DCs derived from monocytes (moDCs) in the development of inflammation has been emphasized. Several studies have shown that the function of DCs can be influenced by gut microbes including gut bacteria and viruses. Abnormal changes/reactions in intestinal DCs are potentially associated with diseases such as inflammatory bowel disease (IBD) and intestinal tumors, allowing DCs to be a new target for the treatment of these diseases. In this review, we summarized the physiological functions of DCs in the intestinal micro-environment, their regulatory relationship with intestinal microorganisms and their regulatory mechanism in intestinal diseases.
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http://dx.doi.org/10.3390/cells10030583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999753PMC
March 2021

Rhubarb extract relieves constipation by stimulating mucus production in the colon and altering the intestinal flora.

Biomed Pharmacother 2021 Jun 25;138:111479. Epub 2021 Mar 25.

Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China. Electronic address:

Background: Constipation, mainly characterized by the difficulty in defecation, is a clinical symptom caused by a variety of factors. It can be manifested as normal or slow colonic transport abnormalities, which can occur alone or concurrently with defecation disorders. As there is not uniform definition and assessment standard, no clear plan could be used for the treatment of constipation. Although rhubarb, a traditional Chinese medicine, plays a therapeutic role in diseases involving constipation symptoms, the detailed mechanism of it in treating constipation remains unclear.

Methods: A model of constipation-induced by diphenoxylate was prepared. Immunofluorescent staining was used to detect the expression of mucin 2 (MUC2), calnexin and chymase in colon. Western blotting was used to detect changes of tryptase and calnexin in the colon. And real-time polymerase chain reaction (PCR) was utilized to detect the changes of immunoglobulin-binding protein (Bip), X-box binding protein 1 (Xbp1) and C/EBP homologous protein (CHOP) of colonic goblet cells in mRNA levels. ELISA and biochemical kits were utilized to detect the changes of MUC2, Trefoil factor 3 (TFF3), acetylcholine, histamine and C-C motif chemokine ligand 5 (CCL5) in the colon. And the changes of colonic mucosa and intestinal flora of constipation model mice caused by rhubarb extract (RE) were analyzed to identify the mechanism of RE on the treatment of constipation.

Results: RE promotes the secretion of colonic mucus by recruiting mast cells and enhancing the content of histamine and Ach in the mice colon. In the process, RE causes up-regulation of Bip and CHOP mRNA expression and down-regulation of Xbp1 and Xbp1s mRNA expression that induces ER stress of colonic epithelium associated with changes in the intestinal flora diversity and short-chain fatty acids content.

Conclusion: RE could relieve constipation by promoting the secretion of colonic mucus via mast cells activation and improving the intestinal microenvironment.
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http://dx.doi.org/10.1016/j.biopha.2021.111479DOI Listing
June 2021

Biological characteristics of IL-6 and related intestinal diseases.

Int J Biol Sci 2021 1;17(1):204-219. Epub 2021 Jan 1.

Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.

The intestine serves as an important digestive and the largest immune organ in the body. Interleukin-6(IL-6), an important mediator of various pathways, participates in the interactions between different kinds of cells and closely correlates with intestinal physiological and pathological condition. In this review we summarize the signaling pathways of IL-6 and its functions in maintaining intestinal homeostasis. We also explored its relation with nervous system and highlight its potential role in Parkinson's disease. Based on its specialty of the double-side influences on intestinal tumors and inflammation, we summarize how they are done through distinctive process.
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http://dx.doi.org/10.7150/ijbs.51362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757046PMC
January 2021

The complete chloroplast genome of R. Br. () and its phylogenetic implication.

Mitochondrial DNA B Resour 2020 Jan 22;5(1):766-767. Epub 2020 Jan 22.

Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, China.

The genus () is an annual parasitic twining herb. There are about 200 species in this genus, which are widely distributed in tropical and subtropical areas. is mainly parasitic on crops bringing significant losses to the production of agriculture. Furthermore, dried seeds of and are used as a Chinese traditional herbal medicine. Despite the importance of species, it is difficult to distinguish these plants by the naked eye. Moreover, plastid sequence information available for species is limited. In this study, the complete chloroplast (cp) genome sequence of was determined using next-generation sequencing. The entire cp genome was determined to be 85,263 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 50,384 and 6727 bp, respectively, which were separated by a pair of 14,076 bp inverted repeat (IR) regions. The genome contained 98 genes, including 61 protein-coding genes, 29 tRNA genes, and 4 rRNA genes. The overall GC content of the genome is 37.8%. A phylogenetic tree reconstructed by 26 chloroplast genomes reveals that is most related with in , with bootstrap support values of 100%.
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http://dx.doi.org/10.1080/23802359.2020.1715865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748759PMC
January 2020

Inherited causes of clonal haematopoiesis in 97,691 whole genomes.

Nature 2020 10 14;586(7831):763-768. Epub 2020 Oct 14.

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA.

Age is the dominant risk factor for most chronic human diseases, but the mechanisms through which ageing confers this risk are largely unknown. The age-related acquisition of somatic mutations that lead to clonal expansion in regenerating haematopoietic stem cell populations has recently been associated with both haematological cancer and coronary heart disease-this phenomenon is termed clonal haematopoiesis of indeterminate potential (CHIP). Simultaneous analyses of germline and somatic whole-genome sequences provide the opportunity to identify root causes of CHIP. Here we analyse high-coverage whole-genome sequences from 97,691 participants of diverse ancestries in the National Heart, Lung, and Blood Institute Trans-omics for Precision Medicine (TOPMed) programme, and identify 4,229 individuals with CHIP. We identify associations with blood cell, lipid and inflammatory traits that are specific to different CHIP driver genes. Association of a genome-wide set of germline genetic variants enabled the identification of three genetic loci associated with CHIP status, including one locus at TET2 that was specific to individuals of African ancestry. In silico-informed in vitro evaluation of the TET2 germline locus enabled the identification of a causal variant that disrupts a TET2 distal enhancer, resulting in increased self-renewal of haematopoietic stem cells. Overall, we observe that germline genetic variation shapes haematopoietic stem cell function, leading to CHIP through mechanisms that are specific to clonal haematopoiesis as well as shared mechanisms that lead to somatic mutations across tissues.
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http://dx.doi.org/10.1038/s41586-020-2819-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944936PMC
October 2020

Dynamic incorporation of multiple in silico functional annotations empowers rare variant association analysis of large whole-genome sequencing studies at scale.

Nat Genet 2020 09 24;52(9):969-983. Epub 2020 Aug 24.

Department of Data Sciences, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Large-scale whole-genome sequencing studies have enabled the analysis of rare variants (RVs) associated with complex phenotypes. Commonly used RV association tests have limited scope to leverage variant functions. We propose STAAR (variant-set test for association using annotation information), a scalable and powerful RV association test method that effectively incorporates both variant categories and multiple complementary annotations using a dynamic weighting scheme. For the latter, we introduce 'annotation principal components', multidimensional summaries of in silico variant annotations. STAAR accounts for population structure and relatedness and is scalable for analyzing very large cohort and biobank whole-genome sequencing studies of continuous and dichotomous traits. We applied STAAR to identify RVs associated with four lipid traits in 12,316 discovery and 17,822 replication samples from the Trans-Omics for Precision Medicine Program. We discovered and replicated new RV associations, including disruptive missense RVs of NPC1L1 and an intergenic region near APOC1P1 associated with low-density lipoprotein cholesterol.
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http://dx.doi.org/10.1038/s41588-020-0676-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483769PMC
September 2020

Baicalin relieves neuropathic pain by regulating α-adrenoceptor levels in rats following spinal nerve injury.

Exp Ther Med 2020 Sep 17;20(3):2684-2690. Epub 2020 Jul 17.

Department of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.

In the present study, the ability of baicalin to relieve neuropathic pain due to spinal nerve ligation in rats was explored, and the relationship between baicalin and α-adrenoceptors (α-AR) was determined. The neuropathic pain model was established by ligating the L5-L6 spinal nerves in Sprague-Dawley rats. Several α-AR antagonists were injected into the intramedullary sheath to evaluate the role of baicalin in neuropathic pain. The antagonists included nonselective α-AR antagonist idazoxan, α-AR antagonist BRL 44408, α-AR antagonist ARC 239 and α-AR antagonist JP 1302. The rats were divided into an untreated control group, saline group, baicalin group and baicalin + α-AR antagonist groups. Paw withdrawal threshold (PWT) was tested to assess the level of pain felt by the rats. The levels of α-AR mRNA were tested by reverse transcription-quantitative PCR. Inflammatory factors, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17 and IL-1β, were analyzed by ELISA. The histopathological changes were assessed by hematoxylin and eosin staining. Flow cytometry was used to examine the percentage of CD4 peripheral blood mononuclear cells (PBMCs). Compared with the saline group, the PWT value increased after treating with baicalin. However, intrathecal injection of α-AR antagonist reversed the antinociceptive effects of baicalin. Compared with the saline group, the expression of α-AR and α-AR mRNA was upregulated significantly in the baicalin group (P<0.05). Levels of α-AR mRNA were also decreased in the baicalin + idazoxan group compared with the baicalin group (P<0.05). The levels of TNF-α, IL-6, IL-17 and IL-1β were raised after treatment with baicalin. In addition, baicalin treatment ameliorated the histological damage in the spinal cord. The percentage of CD4 PBMCs was increased in the saline group compared with the control group (P<0.05). Compared with the baicalin group, the percentage of CD4+ PBMCs was raised after treatment with the α-AR antagonists. In conclusion, intrathecal injection of baicalin produced an antiallodynic effect in a spinal nerve ligation-induced neuropathic pain model. The mechanism may be related to the regulation of a-AR expression.
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http://dx.doi.org/10.3892/etm.2020.9019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401858PMC
September 2020

How to choose kinematic or mechanical alignment individually according to preoperative characteristics of patients?

BMC Musculoskelet Disord 2020 Jul 7;21(1):443. Epub 2020 Jul 7.

Department of Orthopaedic Surgery, ZiBo central hospital, 54 Gong Qingtuan West Road, Zhangdian, Zibo, 255036, Shandong, People's Republic of China.

Background: Making decisions in alignment techniques in total knee arthroplasty (TKA) remains controversial. This study aims to identify the potential patients who were suitable for the kinematic (KA) or mechanical alignment (MA).

Methods: We reviewed 296 consecutive patients (296 TKAs, including 114 KA-TKAs and 182 MA-TKAs) who underwent unilateral TKA using a computer-assisted navigation from 2016 to 2018 in our prospectively maintained database. The minimum followup was 1 year. Clinical outcomes including the range of motion (ROM) and knee society score (KSS) were compared between KA-TKAs and MA-TKAs. Multiple regression models were used to evaluate the relationship between alignment techniques and KSS at the 1-year followup. Interaction and stratified analyses were conducted according to gender, age, body mass index (BMI), preoperative hip-knee-ankle (HKA) angle, ROM and KSS.

Results: ROM and KSS at the 1-year followup didn't differ between MA-TKAs and KA-TKAs (all p > 0.05). Alignment techniques did not associate with postoperative ROM (Adjusted β = 0.4, 95% confidence interval [CI]: - 0.3, 1.6; p = 0.752) or 1-year KSS (Adjusted β = 2.2, 95%CI: - 0.7, 5.6; p = 0.107). Patients with a BMI more than 30 kg/m^2 achieved better 1-year KSS when using MA than KA (p for interaction< 0.05). Additionally, patients with preoperative HKA angle more than 10 degrees varus benefited more from KA than MA (p for interaction< 0.05).

Conclusions: Patients with severe varus deformity may be suitable for the KA technique, whereas MA should be used in obese patients.
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http://dx.doi.org/10.1186/s12891-020-03472-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341594PMC
July 2020

[Protective effect of water extracts of Orychophragmus violaceus seeds on TAA-induced acute liver injury in mice].

Zhongguo Zhong Yao Za Zhi 2020 Mar;45(6):1399-1405

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.

This study aimed to investigate the protective effect of water extracts of Orychophragmus violaceus seeds on liver injury induced by thioacetamide(TAA) in mice. ICR male mice were randomly divided into seven groups: normal group, model group, bicyclol positive control group(200 mg·kg~(-1)), Kuihua Hugan Tablets group(350 mg·kg~(-1)), O. violaceus seeds low-dose water extract group(125 mg·kg~(-1)), middle-dose water extract group(250 mg·kg~(-1)), and high-dose water extract group(500 mg·kg~(-1)). Intragastric administration was given in all groups at 0.02 mL·g~(-1) body weight, 1 time a day for continuous 4 days. One h after the administration on the 4 th day, the liver injury model was induced by intraperitoneal injection of TAA(100 mg·kg~(-1)). The mice were put to death 24 hours later. Blood and tissues were taken and organ indexes were calculated. The activities of ALT, AST and TBiL in serum were detected. The content of MDA, GSH and the activity of SOD, GSH-Px in liver homogenate were examined by colorimetry method. HE staining was used to observe the pathological changes of liver tissues in mice. The protein expression levels of NF-κB p65, Keap-1, Nrf2, p-p38, p-JNK, p-ERK, Bax, Bcl-2, caspase-3, cleaved caspase-3 and caspase-8 were detected by Western blot. The results showed that as compared with the model group, various O. violaceus seeds groups could significantly improve the pathological conditions of liver and reduce ALT, AST, TBiL activities in serum of mice with liver injury. In the high-dose group, the activities of SOD, GSH-Px and the content of GSH were significantly increased, while MDA content was sharply declined. Meanwhile, O. violaceus seeds extract down-regulated the expressions of Bax, Keap-1, p-p38, p-JNK, p-ERK, NF-κB p65, cleaved caspase-3 and up-regulated the expressions of Nrf2, Bcl-2, caspase-3 and caspase-8. In conclusion, O. violaceus seeds extract exhibited potent protective effect on liver injury induced by TAA in mice, and its mechanism may be related to down-regulating levels of Keap-1, up-regulating the expressions of Nrf2, inhibiting the expressions of p-p38, p-ERK and NF-κB p65 signaling pathway, and inhibiting hepatocyte apoptosis by down-regulating the expressions of p-JNK and Bax and up-regulating the expressions of Bcl-2.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20191112.405DOI Listing
March 2020

[Corticotrophin-releasing hormone neurons in the central amygdala mediate morphine withdrawal-induced negative emotions].

Sheng Li Xue Bao 2019 Dec;71(6):824-832

The State Key Laboratory of Medical Neurobiology and Pharmacology Research Center, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Drugs of abuse leads to adaptive changes in the brain stress system, and produces negative affective states including aversion and anxiety after drug use is terminated. Corticotrophin-releasing hormone (CRH) is the main transmitter in control of response to stressors and is neuronal enriched in the central amygdala (CeA), a sub-region of the extended amygdala playing an important role in integrating emotional information and modulating stress response. The effect of CRH neurons in CeA on the negative emotions on morphine naïve and withdrawal mice is unclear. Thus, we utilized CRH-Cre transgenic mice injected with AAV-mediated Designer Receptors Exclusively Activated By Designer Drugs (DREADDs) to chemogenetically manipulate CRH neurons in CeA. And methods of behavior analysis, including conditioned place aversion (CPA), elevated plus maze and locomotor activity tests, were used to investigate morphine withdrawal-induced negative emotions in mice. The results showed that, inhibiting CRH neurons of CeA decreased the formation of morphine withdrawal-induced CPA, as well as the anxiety level of CRH-Cre mice. Furthermore, specifically activating CRH neurons in CeA evoked CPA and anxiety of morphine naïve mice. Neither inhibiting nor activating CRH neurons had effects on their locomotor activity. These results suggest that CRH neurons in CeA are involved in the mediation of morphine withdrawal-induced negative emotion in mice, providing a theoretical basis for drug addiction and relapse mechanism.
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December 2019

Characteristics of Changes in Inflammatory Cytokines as a Function of Hepatic Ischemia-Reperfusion Injury Stage in Mice.

Inflammation 2019 Dec;42(6):2139-2147

Liver Transplantation Center, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.

Liver ischemia-reperfusion injury (IRI) can severely compromise the prognosis of patients receiving liver surgery. While inflammation contributes to the damage resulting from IRI, only a limited number of inflammation biomarkers have been identified as being associated with the different stages of hepatic IRI. As an approach to identify some of these inflammatory cytokines and the molecular mechanisms involved within different stages of hepatic IRI, we used an advanced antibody array assay to detect multiple proteins. With this technology, we observed specific differences in the content of inflammatory cytokines between ischemic and sham controls, as well as a function of the different reperfusion stages in a hepatic IRI mouse model. For example, while liver tissue content of IL-12p40/p70 was significantly increased in the ischemic stage, it was significantly decreased in the reperfusion stage as compared with that of the sham group. For other inflammatory cytokines, no changes were obtained between the ischemic and reperfusion stages with levels of IL-17, Eotaxin-2, Eotaxin, and sTNF RII all being consistently increased, while those of TIMP-1, TIMP-2, BLC, and MCSF consistently decreased as compared with that of the sham group at all reperfusion stages examined. Results from protein function annotation Gene Ontology and the KEGG pathway revealed that inflammatory cytokines are enriched in a network associated with activation of inflammatory response signaling pathways such as TLR, TNF, and IL-17 when comparing responses of the IR versus sham groups. The identification of cytokines along with their roles at specific stages of IRI may reveal important new biological markers for the diagnosis and prognosis of hepatic IRI.
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http://dx.doi.org/10.1007/s10753-019-01078-yDOI Listing
December 2019

Preparation and Characterization of AMT/Co(acac)-Loaded PAN/PS Micro-Nanofibers with Large through-Pores.

Nanoscale Res Lett 2019 Aug 20;14(1):290. Epub 2019 Aug 20.

National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.

This study focused on the fabrication and characterization of ammonium metatungstate hydrate (AMT) combined with cobalt(III) acetylacetonate (Co(acac))-loaded electrospun micro-nanofibers. The morphologies, structures, element distribution, through-pore size, and through-pore size distribution of AMT/Co(acac)-loaded PAN/PS micro-nanofibers were investigated by a combination of field emission scanning electron microscopy (FESEM), flourier transformation infrared (FTIR) spectroscopy, energy disperse spectroscopy (EDS), through-pore size analyzer, and so on. These micro-nanofibers have many advantages in their potential application as electro-catalysts. The porous and large thorough-pore will benefit for effective electrolyte penetration, in addition to promoting gas bubbles evolving and releasing from catalyst surface timely.
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http://dx.doi.org/10.1186/s11671-019-3059-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702242PMC
August 2019

Exercise improves depressive symptoms by increasing the number of excitatory synapses in the hippocampus of CUS-Induced depression model rats.

Behav Brain Res 2019 11 29;374:112115. Epub 2019 Jul 29.

Department of Histology and Embryology, Chongqing Medical University, Chongqing, 400016, PR China; Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address:

Exercise has been considered for the treatment of depression, but the mechanism by which exercise improves depression is still unclear. To clarify the mechanism, rats were randomly divided into the control, chronic unpredictable stress (CUS)/standard and CUS/running groups. The rats in the CUS/running group ran for four weeks. In this study, a sucrose preference test (SPT) was used to evaluate the depression-like symptoms in the rats, and western blot, immunohistochemical and stereological analyses were performed to study the expression of synaptic-related proteins in the hippocampus and the changes in excitatory synapses in each sub-region. The results show that sucrose preference in the CUS/standard group was significantly lower than that in the control group, but in the CUS/running group, sucrose preference was higher than that in the CUS/standard group. Surprisingly, there was no difference in the synaptic-related proteins in the hippocampus among groups. The CUS/standard group exhibited fewer spinophilin (Sp) dendritic spines representing excitatory synapses in CA1, CA3 and dentate gyrus (DG) of the hippocampus than the control group, whereas the CUS/running group exhibited significantly more Sp dendritic spines in these regions than the CUS/standard group, indicating that excitatory synapses were reduced in depressed rats and that running exercises could reverse this change. We hypothesize that the changes in the number of excitatory synapses better reflect the changes in depressive symptoms than the level of synaptic proteins and that the effect of exercise on excitatory synapses in the sub-regions of the hippocampus may be an important structural indicator of the improvement of depressive symptoms.
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http://dx.doi.org/10.1016/j.bbr.2019.112115DOI Listing
November 2019

MiR-223 regulates proliferation and apoptosis of IL-22-stimulated HaCat human keratinocyte cell lines via the PTEN/Akt pathway.

Life Sci 2019 Aug 17;230:28-34. Epub 2019 May 17.

Department of Dermatovenereology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Psoriasis, a chronic inflammatory skin disorder disease, is closely associated with hyperproliferation of keratinocytes. Upregulated miR-223 has been found in peripheral blood mononuclear cells from patients with psoriasis and from psoriatic skin. However, its role in keratinocytes remains unknown. We thus aimed to investigate the function of miR-223 in psoriasis. Interleukin-22 (IL-22) is a crucial keratinocyte trigger in the T-cell-mediated immune response to psoriasis. We found miR-223 to be overexpressed in psoriatic lesions and in IL-22-stimulated HaCaT cells. HaCaT cells then were transfected with a miR-223 mimic or inhibitor to overexpress or inhibit expression of miR-223, respectively. A Cell Counting Kit-8 assay revealed that miR-223 overexpression promoted and miR-223 downregulation inhibited proliferation in IL-22-stimulated HaCaT cells. Flow cytometry analysis certified that miR-223 overexpression decreased HaCaT cell apoptosis, whereas miR-223 downregulation increased it. A dual-luciferase reporter assay demonstrated that miR-223 directly targeted the phosphatase and tensin homolog (PTEN) gene. MiR-223 also negatively regulated mRNA and protein expression of PTEN and modulated the PTEN/Akt pathway in IL-22-stimulated HaCaT cells. PTEN silencing attenuated the activity of the miR-223 inhibitor in these cells via the PTEN/Akt pathway. Overall, the results showed that miR-223 increased proliferation and inhibited apoptosis of IL-22-stimulated keratinocytes via the PTEN/Akt pathway.
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http://dx.doi.org/10.1016/j.lfs.2019.05.045DOI Listing
August 2019

The Study on Semi-Blunt Puncture Behavior of Nanofiber Membrane/ Non-Woven Composite Material.

Recent Pat Nanotechnol 2019 ;13(1):70-76

Key Laboratory of Eco-textiles, Ministry of Education, Jiangnan University, Jiangsu, China.

Background: Nanofiber membrane/non-woven composite material is composed of electrospinning nanofiber membrane and non-woven fabric, which combines the supporting role of nonwoven material and the special nano-size effect of nanomaterials.

Objective: These composite material can be widely used in biomedical, filtration and other related fields. In the actual use process, nanofiber membrane/non-woven composite material is often subjected to external forces such as puncture or bursting. As a result, the mechanical study of nanofiber membrane/ non-woven composite materials has a high value and practical significance.

Methods: The nanofiber membrane/non-woven composite material was obtained by spraying solution (different concentrations of titanium dioxide-loaded Poly (vinyl alcohol) (PVA)) on meltblown polyester non-woven fabric. The surface morphology and fiber diameter of different concentrations nanotitanium dioxide-loaded Poly (vinyl alcohol) fiber were investigated by Field Emission Scanning Electron Microscopy (FESEM). The surface distribution of TiO2 on the electrospun fibrous membranes was characterized by Energy Disperse Spectroscopy (EDS). The semi-blunt puncture behavior of different concentrations of nano-titanium dioxide-loaded nanofiber membrane/non-woven composite material was conducted by universal material machine.

Results: With the increase of concentrations of nano-titanium dioxide particles, the surface smoothness of nanofibers diminishes, the unevenness of the diameter distribution of the fiber increased and the maximum semi-blunt puncture strength increased.

Conclusion: The addition of hard particles does contribute to improving the puncture properties of the composite materials. Several patents, related to electrospinning and bubble electrospinning equipment for nanofiber fabrication, have been reported.
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http://dx.doi.org/10.2174/1872210513666190204153744DOI Listing
January 2019

Nonvolatile and Reversible Ferroelectric Control of Electronic Properties of BiTe Topological Insulator Thin Films Grown on Pb(MgNb)O-PbTiO Single Crystals.

ACS Appl Mater Interfaces 2019 Mar 20;11(9):9548-9556. Epub 2019 Feb 20.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics , Chinese Academy of Sciences , Shanghai 200050 , China.

Single-phase (00 l)-oriented BiTe topological insulator thin films have been deposited on (111)-oriented ferroelectric 0.71Pb(MgNb)O-0.29PbTiO (PMN-PT) single-crystal substrates. Taking advantage of the nonvolatile polarization charges induced by the polarization direction switching of PMN-PT substrates at room temperature, the carrier density, Fermi level, magnetoconductance, conductance channel, phase coherence length, and quantum corrections to the conductance can be in situ modulated in a reversible and nonvolatile manner. Specifically, upon the polarization switching from the positively poled P state (i.e., polarization direction points to the film) to the negatively poled P (i.e., polarization direction points to the bottom electrode) state, both the electron carrier density and the Fermi wave vector decrease significantly, reflecting a shift of the Fermi level toward the Dirac point. The polarization switching from P to P also results in significant increase of the conductance channel α from -0.15 to -0.3 and a decrease of the phase coherence length from 200 to 80 nm at T = 2 K as well as a reduction of the electron-electron interaction. All these results demonstrate that electric-voltage control of physical properties using PMN-PT as both substrates and gating materials provides a simple and a straightforward approach to realize reversible and nonvolatile tuning of electronic properties of topological thin films and may be further extended to study carrier density-related quantum transport properties of other quantum matter.
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http://dx.doi.org/10.1021/acsami.8b20406DOI Listing
March 2019

[Somatostatin interneurons of prelimbic cortex in regulating morphine-induced behavior changes].

Sheng Li Xue Bao 2018 Oct;70(5):463-473

The State Key Laboratory of Medical Neurobiology and Pharmacology Research Center, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Drug addiction is a chronic psychiatric disorder characterized by compulsive drug taking, and involves neuronal plasticity changes in multiple brain regions. The prelimbic cortex (PrL) is a key region of the dorsomedial prefrontal cortex and contains majority of pyramidal neurons. The excitatory projections from PrL play a very important role in the drug seeking behaviors. PrL also contains a small amount of GABAergic interneurons, which regulate the information integration and transmission of the pyramidal neurons. However, the roles of the GABAergic interneurons in PrL in drug-induced behavior changes are not clear. In the PrL, parvalbumin (PV) and somatostatin (SST) interneurons are two major GABAergic interneurons, which have been reported to regulate the activity of glutamatergic input, and form inhibitory synaptic transmission to regulate the output of downstream signals. Here, we used PV-Cre and SST-Cre mice combined with chemical genetics to explore the role of PV and SST interneurons in PrL in morphine-induced behavior changes. Our data showed that specific inhibiting SST interneurons in PrL significantly increased the anxiety level and decreased morphine-induced locomotor activity and the conditioned place preference (CPP) score. Instead, specific inhibiting PV interneurons in PrL had no effect on the anxiety level, morphine induced-locomotor activity and CPP. Our findings provide a new insight into the cellular and neuronal specific mechanism for drug addiction.
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October 2018

Bioinspired Transition-Metal Complexes as Electrocatalysts for the Oxygen Reduction Reaction.

Chemistry 2019 Mar 21;25(15):3726-3739. Epub 2018 Dec 21.

Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.

The oxygen reduction reaction (ORR) is one of the most important reactions in life processes and energy conversion systems. To alleviate global warming and the energy crisis, the development of high-performance electrocatalysts for the ORR for application in energy conversion and storage devices such as metal-air batteries and fuel cells is highly desirable. Inspired by the biological oxygen activation/reduction process associated with heme- and multicopper-containing metalloenzymes, iron and copper-based transition-metal complexes have been extensively explored as ORR electrocatalysts. Herein, an outline into recent progress on non-precious-metal electrocatalysts for the ORR is provided; these electrocatalysts do not require pyrolysis treatment, which is regarded as desirable from the viewpoint of bioinspired molecular catalyst design, focusing on iron/cobalt macrocycles (porphyrins, phthalocyanines, and corroles) and copper complexes in which the ORR activity is tuned by ligand variation/substitution, the method of catalyst immobilization, and the underlying supporting materials. Current challenges and exciting imminent developments in bioinspired ORR electrocatalysts are summarized and proposed.
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http://dx.doi.org/10.1002/chem.201803764DOI Listing
March 2019

Imaging Features of the Whole Uterus Volume CT Perfusion and Influence Factors of Blood Supply: A Primary Study in Patients with Cervical Squamous Carcinoma.

Acad Radiol 2019 08 8;26(8):e216-e223. Epub 2018 Sep 8.

Department of Radiology, Cancer Hospital of China Medical University, LiaoNing Cancer Hospital &Institute, 44# Xiao He Yan Road, Shenyang, Liaoning 110042, China. Electronic address:

Rationale And Objectives: To explore the imaging features of whole uterus volume CT perfusion (vCTP) and the influence factors of blood supply in cervical squamous carcinoma (CSC).

Materials And Methods: vCTP was performed on a 640-slice computed tomography system in 43 patients with CSC diagnosed by biopsy, and 24 cases of them underwent magnetic resonance imaging. The size of the tumor was measured on vCTP and magnetic resonance (MR) images. Perfusion parameters, including arterial blood flow (AF), blood volume, and permeability surface (PS), were measured by two radiologists, using interclass correlation coefficient to evaluate the interobserver reliability. The difference of tumor size and perfusion data was analyzed by paired t test and rank sum test. The correlation of perfusion parameters with some factors was analyzed by Pearson or Spearman correlation analysis.

Results: Tumor sizes were not significantly different between vCTP and MR images. The interclass correlation coefficient of each parameter was 0.818-0.945. The AF value of CSC was significantly higher than normal uterine body, and the blood volume and PS values of CSC were not statistically different compared with those of normal uterine body. There was no significant difference in AF value of CSC among different FIGO stages and pathological grades. The AF and PS values of CSC were negatively correlated with the age of the patients.

Conclusion: The vCTP could accurately shows the size of the CSC with use of MR as the reference standard, and its perfusion parameters have good measurement stability; the CSC was hypervascular, but this trend was less pronounced in older women.
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http://dx.doi.org/10.1016/j.acra.2018.07.015DOI Listing
August 2019

Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients.

Cancer Med 2018 09 23;7(9):4650-4664. Epub 2018 Jul 23.

The Second General Surgery, Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital, Shijiazhuang, Hebei, China.

Long noncoding RNAs (lncRNAs) play an important role in gene regulation, but their impact on the pathogenesis of colorectal cancer and the biological function of cancer cells is unclear. In this study, we used next-generation sequencing to study the differences in the expression profiles of lncRNAs and mRNAs in colorectal cancer tissues. We analyzed the differentially expressed genes by Gene Ontology/Kyoto Encyclopedia of Genes and Genomes (GO/KEGG) enrichment and predicted new lncRNA functions. Our results revealed that compared with lncRNAs and mRNAs in nontumor colorectal tissues, 1019 lncRNAs (512 upregulated, 507 downregulated) and 3221 mRNAs (1606 upregulated, 1615 downregulated) were differentially expressed in tumor colorectal tissues (fold change >2 and P < 0.05). We validated some of these genes by qPCR. Furthermore, we identified some new lncRNAs differently expressed in colorectal cancer samples from patients in northern China. We confirmed the function of lncRNA-FIRRE-201 and SLCO4A1-AS1-202 in colorectal cancer cells to provide an experimental basis for studies on their roles in the occurrence and development of colorectal cancer and in the regulation of networks.
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http://dx.doi.org/10.1002/cam4.1696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6144144PMC
September 2018

[Spatial Distribution, Source and Ecological Risk Assessment of Heavy Metals in the Coastal Sediments of Northern Dongying City].

Huan Jing Ke Xue 2017 Sep;38(9):3635-3644

Key Laboratory for Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.

Concentrations of seven heavy metals in the coastal sediments of the northern Dongying City were measured. The spatial distribution and sources of heavy metals were discussed and the ecological risk was assessed using sediment quality guidelines (SQGs) and the potential ecological risk index (PERI). The concentration ranges of Hg, As, Pb, Cd, Cr, Cu, and Zn were calculated as 0.005-0.092, 3.44-10.41, 6.59-19.00, 0.50-1.10, 32.42-60.25, 1.72-23.78, and 31.13-69.96 mg·kg, respectively. Higher metal concentrations were observed at site S10, which was close to the mouth of Tiaohe River and contained plenty of organic matter, silt, and clay. Principal component analysis (PCA) indicated that As and Pb in the sediments were derived from natural weathering processes, while other metals were mainly attributed to anthropogenic sources, i.e., land-sourced pollutants transported by runoff. Concentrations of As, Cd, Cr, and Cu at some sampling sites exceeded the threshold effect level (TEL) stated in the SQGs implying occasional harmful effects on biological life. PERI showed that the sediments in this area generally had a medium risk, except sites S10, S3, and S9 posing a considerable risk, and that Cd and Hg were the major contributors to the ecological risk. It is necessary to take effective measures to control heavy metal fluxes from rivers around this area and to reduce the risk.
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http://dx.doi.org/10.13227/j.hjkx.201701048DOI Listing
September 2017

Medicinal mushroom Phellinus igniarius induced cell apoptosis in gastric cancer SGC-7901 through a mitochondria-dependent pathway.

Biomed Pharmacother 2018 Jun 14;102:18-25. Epub 2018 Mar 14.

School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, PR China. Electronic address:

Gastric cancer (GC) is the fourth most common diagnosed cancer and the second leading cause of cancer death worldwide. Phellinus igniarius is a traditional medicinal mushroom used in China and other countries of East Asia for the treatment of various diseases including cancer. The aim of this study is to evaluate the antitumor activity of P. igniarius and elucidate the possible mechanism. MTT assay displayed that the total ethanol extract of P. igniarius (TPI) had antitumor activities against five human tumor cell lines of HepG-2, AGS, SGC-7901, Hela and A-549. TPI was found the most cytotoxity against gastric cancer SGC-7901 in vitro, and strongly inhibited the tumor growth in xenograft nude mice in vivo. Typical morphological changes due to cell apoptosis including chromatin condensation, and nuclear fragmentation with the formation of apoptotic bodies were observed in the SGC-7901 cells after TPI treatment. TPI blocked SGC-7901 cell cycle at G/G phase and caused apoptosis by down-regulated the expression of cyclin D1. TPI caused a remarkable collapse of mitochondrial membrane potential (MMP, △ψm) in SGC-7901 cells and induced the mitochondrial-dependent apoptosis by triggered the caspase-9, -3 activation and PARP cleavage. Moreover, TPI increased the ratio of Bax/Bcl-2 in SGC-7901 both in vitro and in vivo. These findings suggested that P. igniarius could be a potential natural derived therapeutic agent for the prevention and treatment of gastric cancer, as it could induce the cancer cell apoptosis through a mitochondria-dependent pathway.
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http://dx.doi.org/10.1016/j.biopha.2018.03.038DOI Listing
June 2018

A comparison of diagnostic performance of vacuum-assisted biopsy and core needle biopsy for breast microcalcification: a systematic review and meta-analysis.

Ir J Med Sci 2018 Nov 16;187(4):999-1008. Epub 2018 Mar 16.

The Second Department of General Surgery, Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital, No. 12 Jiankang Road, Shijiazhuang, China.

Background: Core needle biopsy (CNB) and vacuum-assisted biopsy (VAB) are both popularly used breast percutaneous biopsies. Both of them have become reliable alternatives to open surgical biopsy (OSB) for breast microcalcification (BM).

Aims: It is controversial that which biopsy method is more accurate and safer for BM. Hence, we conducted this meta-analysis to compare the diagnostic performance between CNB and VAB for BM, aiming to find out the better method.

Methods: Articles according with including and excluding criteria were collected from the databases, PubMed, Embase, and the Cochrane Library. Preset outcomes were abstracted and pooled to find out the potential advantages in CNB or VAB.

Results: Seven studies were identified and entered final meta-analysis from initially found 138 studies. The rate of ductal carcinoma in situ (DCIS) underestimation was significantly lower in VAB than CNB group [risk ratio (RR) = 1.83, 95% confidence interval (CI) 1.40 to 2.40, p < 0.001]. The microcalcification retrieval rate was significantly higher in VAB than CNB group (RR = 0.89, 95% CI 0.81 to 0.98, p = 0.02), while CNB owned a significantly lower complication rate than VAB (RR = 0.18, 95% CI 0.03 to 0.93, p = 0.04). The atypical ductal hyperplasia (ADH) underestimation rates were not compared for the limited number of studies reporting this outcome.

Conclusions: Compared with CNB, VAB shows better diagnostic performance in DCIS underestimation rate and microcalcification retrieval rate. However, CNB shows a significantly lower complication rate. More studies are needed to verify these findings.
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http://dx.doi.org/10.1007/s11845-018-1781-6DOI Listing
November 2018

Cytotoxic Activities of Fractions of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), and the Induction of Cell Cycle Arrest and Apoptosis in MGC-803 Cells.

Int J Med Mushrooms 2017 ;19(6):561-570

Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China.

Phellinus igniarius, a perennial medicinal mushroom, has been used in China and other countries of East Asia for the treatment of various diseases, including cancer. The purpose of this study is to evaluate the cytotoxic activities of different fractions of an ethanol extract from Ph. igniarius and to elucidate a possible antitumor mechanism. An ethanol extract of Ph. igniarius was partitioned into a petroleum ether fraction, an ethyl acetate fraction (EAF), an n-butanol fraction, and a water-soluble fraction. The cytotoxic activity of all the fractions was initially screened in a brine shrimp lethality test, then evaluated by the Cell Counting Kit-8 assay against 5 human tumor cell lines: MGC-803, BEL-7402, HeLa, MCF-7, and HCT-116. The cell cycle distribution induced by EAF on MGC-803 cells was analyzed by flow cytometry with propidium iodide staining, and apoptosis was determined using flow cytometry with Annexin V/propidium iodide staining. The results of the brine shrimp lethality test and the Cell Counting Kit-8 assay showed that EAF was the most active fraction, displaying strong inhibitory activity against the MGC-803, BEL-7402, and MCF-7 cancer cell lines. Flow cytometry analysis indicated that EAF could induce S-phase cell cycle arrest in MGC-803 cells and cause apoptosis in a concentration-dependent manner. This study demonstrated that EAF, as the most active fraction of Ph. igniarius, exerted antitumor activity by inducing MGC-803 cell apoptosis via S-phase cell cycle arrest.
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http://dx.doi.org/10.1615/IntJMedMushrooms.v19.i6.70DOI Listing
July 2018

Methylation of transcription factor YY2 regulates its transcriptional activity and cell proliferation.

Cell Discov 2017 3;3:17035. Epub 2017 Oct 3.

School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research, Xiamen University, Fujian, China.

Yin Yang 1 (YY1) is a multifunctional DNA-binding transcription factor shown to be critical in a variety of biological processes, and its activity and function have been shown to be regulated by multitude of mechanisms, which include but are not limited to post-translational modifications (PTMs), its associated proteins and cellular localization. YY2, the paralog of YY1 in mouse and human, has been proposed to function redundantly or oppositely in a context-specific manner compared with YY1. Despite its functional importance, how YY2's DNA-binding activity and function are regulated, particularly by PTMs, remains completely unknown. Here we report the first PTM with functional characterization on YY2, namely lysine 247 monomethylation (K247me1), which was found to be dynamically regulated by SET7/9 and LSD1 both and in cultured cells. Functional study revealed that SET7/9-mediated YY2 methylation regulated its DNA-binding activity and in association with chromatin examined by chromatin immunoprecipitation coupled with sequencing (ChIP-seq) in cultured cells. Knockout of YY2, SET7/9 or LSD1 by CRISPR (clustered, regularly interspaced, short palindromic repeats)/Cas9-mediated gene editing followed by RNA sequencing (RNA-seq) revealed that a subset of genes was positively regulated by YY2 and SET7/9, but negatively regulated by LSD1, which were enriched with genes involved in cell proliferation regulation. Importantly, YY2-regulated gene transcription, cell proliferation and tumor growth were dependent, at least partially, on YY2 K247 methylation. Finally, somatic mutations on YY2 found in cancer, which are in close proximity to K247, altered its methylation, DNA-binding activity and gene transcription it controls. Our findings revealed the first PTM with functional implications imposed on YY2 protein, and linked YY2 methylation with its biological functions.
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http://dx.doi.org/10.1038/celldisc.2017.35DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665210PMC
October 2017

Crassifoside H improve the depressive-like behavior of rats under chronic unpredictable mild stress: Possible involved mechanisms.

Brain Res Bull 2017 Oct 29;135:77-84. Epub 2017 Sep 29.

Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, PR China. Electronic address:

Crassifoside (CH) is a novel chlorine-containing compound isolated from rhizomes of Curculigo glabrescens. This study aimed to explore the antidepressant-like effect of CH and involved mechanisms. A rat depression model was established using chronic unpredictable mild stress (CUMS) paradigm. Behavioral tests including sucrose preference test (SPT), open field test (OFT) and forced swimming test (FST) were used to evaluate the antidepressant-like effect of CH. The levels of plasma corticosterone (CORT) and corticotrophin-releasing factor (CRF) in hypothalamus were measured to determine the activity of hypothalamic pituitary-adrenal (HPA) axis. Protein expression of 5-hydroxytryptamine 1A (5-HT) receptor, brain-derived neurotrophic factor (BDNF), as well as the total and phosphorylated extracellular signal-regulated kinase (ERK)1/2 in hippocampus were also analyzed by Western blotting. The CH administration effectively ameliorated the depressive-like behaviors of CUMS rats, as indicated by the increased sucrose intake in SPT, reduced immobility time in FST, and the increased rearing and grooming numbers, spent more time in inner zone and less time in outer zone in OFT. CH improved CUMS-induced HPA axis hyperactivity by reduced plasma CORT and CRH expression in hypothalamus. Moreover, CH reversed CUMS-induced decrease of 5-HT receptor expression, and up-regulated BDNF and phosphorylated-ERK1/2 levels in hippocampus. These findings suggest that CH improved depressive behaviors of CUMS rats by modulating of HPA axis dysfunction, increasing 5-HT receptor expression, and activating BDNF-ERK signaling pathway.
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http://dx.doi.org/10.1016/j.brainresbull.2017.09.015DOI Listing
October 2017
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