Publications by authors named "Fei Fang"

149 Publications

Unexpected role for IGF-1 in starvation: Maintenance of blood glucose.

Proc Natl Acad Sci U S A 2022 Aug 1;119(32):e2208855119. Epub 2022 Aug 1.

Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390.

Wild-type (WT) mice maintain viable levels of blood glucose even when adipose stores are depleted by 6 d of 60% calorie restriction followed by a 23-h fast (hereafter designated as "starved" mice). Survival depends on ghrelin, an octanoylated peptide hormone. Mice that lack ghrelin suffer lethal hypoglycemia when subjected to the same starvation regimen. Ghrelin is known to stimulate secretion of growth hormone (GH), which in turn stimulates secretion of IGF-1 (insulin-like growth factor-1). In the current study, we found that starved ghrelin-deficient mice had a 90% reduction in plasma IGF-1 when compared with starved WT mice. Injection of IGF-1 in starved ghrelin-deficient mice caused a twofold increase in glucose production and raised blood glucose to levels seen in starved WT mice. Increased glucose production was accompanied by increases in plasma glycerol, fatty acids and ketone bodies, and hepatic triglycerides. All of these increases were abolished when the mice were treated with atglistatin, an inhibitor of adipose tissue triglyceride lipase. We conclude that IGF-1 stimulates adipose tissue lipolysis in starved mice and that this lipolysis supplies energy and substrates that restore hepatic gluconeogenesis. This action of IGF-1 in starved mice is in contrast to its known action in inhibiting adipose tissue lipase in fed mice. Surprisingly, the ghrelin-dependent maintenance of plasma IGF-1 in starved mice was not mediated by GH. Direct injection of GH into starved ghrelin-deficient mice failed to increase plasma IGF-1. These data call attention to an unsuspected role of IGF-1 in the adaptation to starvation.
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http://dx.doi.org/10.1073/pnas.2208855119DOI Listing
August 2022

Relapse of immune thrombotic thrombocytopenic purpura (iTTP) possibly triggered by COVID-19 vaccination and/or concurrent COVID-19 infection.

BMJ Case Rep 2022 Jul 28;15(7). Epub 2022 Jul 28.

Departments of Medicine and Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada

Thrombotic thrombocytopenic purpura (TTP) is a life-threatening disease that may be triggered by inflammation, including infection or vaccination. Since the start of the COVID-19 pandemic, several case reports were published on de novo or relapsed immune TTP (iTTP) in COVID-19-infected patients. Case reports of iTTP episodes following vaccination against COVID-19 are also emerging. We report a case of relapsed iTTP in a patient who received Moderna mRNA-1273 SARS-CoV-2 vaccine and developed concurrent severe COVID-19 infection. The patient's iTTP was successfully managed with caplacizumab, therapeutic plasma exchange and high-dose steroids. We summarise published cases of iTTP associated with COVID-19 infection or vaccination.
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http://dx.doi.org/10.1136/bcr-2021-247524DOI Listing
July 2022

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean.

Int J Mol Sci 2022 Jun 21;23(13). Epub 2022 Jun 21.

National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, , a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of , which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the over-expressing showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of under drought and salinity stresses in soybean.
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http://dx.doi.org/10.3390/ijms23136893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266294PMC
June 2022

Correlation Analysis of CML, sRAGE, and esRAGE and the Measure of Atherosclerosis of Coronary Heart Disease.

Comput Intell Neurosci 2022 10;2022:8970860. Epub 2022 Jun 10.

Department of Cardiology, Xi'an First Hospital, Xi'an 710002, China.

Objective: To investigate the correlation between CML, sRAGE, and esRAGE and the measure of atherosclerosis of coronary heart disease.

Methods: From June 2019 to December 2021, there were 100 patients in all suffering from coronary heart disease (CHD) selected as the observation group. On the basis of Gensini score, they were divided into mild group (Gensini score < 12 points), moderate group (12 points ≤ Gensini score ≤60 points), and severe group (Gensini score > 60). Apart from that, 50 normal people staying in our hospital for physical examination were chosen as the control group in the meantime. N in each group was detected and compared -Carboxymethyl lysine (CML), soluble advanced glycation end product receptor (sRAGE), and endogenous secretory advanced glycation end product receptor (esRAGE). correlation coefficient was adapted to assay the relevance between CML, sRAGE, and esRAGE, as well as the degree of atherosclerosis in CHD. Receiver operator characteristic (ROC) curve was applied to during the evaluation of the diagnosis of CML, sRAGE, and esRAGE, as well as their combined detection of severe atherosclerosis in CHD.

Results: In contrast with the control group, the level of serum CML together with sRAGE in the observation group was considerably elevated, while the level of esRAGE appeared in a downward trend ( < 0.05). The level of serum CML and sRAGE was directly proportional to the measure of atherosclerosis in CHD, while the level of esRAGE was inversely proportional to the measure of atherosclerosis in CHD ( < 0.05). That is to say that serum CML and sRAGE were positive in matter of the measure of atherosclerosis in CHD, while esRAGE negatively appertains to the measure of atherosclerosis in CHD ( < 0.05). Serum CML, sRAGE, and esRAGE could effectively diagnose severe atherosclerosis in CHD, and the combined detection sensitivity (89.79%), specificity (77.16%), accuracy (86.12%), positive predictive value (86.63%), negative predictive value (88.59%), and area under ROC curve (AUC) (0.924) were higher ( < 0.05).

Conclusion: CML and sRAGE, as well as esRAGE, are bound up with the degree of atherosclerosis in CHD, which is conducive to clinical diagnosis and treatment.
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http://dx.doi.org/10.1155/2022/8970860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205688PMC
June 2022

Consumer preference, behaviour and perception about lamb meat in China.

Meat Sci 2022 Oct 5;192:108878. Epub 2022 Jun 5.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China. Electronic address:

A questionnaire including 31 questions was conducted in thirty-one provinces of China's thirty-four provinces. A total of 7212 questionnaires were received in the research and 3775 valid questionnaires were used in the final data analysis. Around 86.0% of the respondents from the selected valid questionnaires liked eating lamb meat. About 66.7% of the respondents from the selected valid questionnaires preferred to use fresh lamb meat for cooking. Stewing was the most popular cooking method for lamb meat consumption in China. Almost 70.0% of the respondents from the selected valid questionnaires preferred to consume local lamb meat rather than imported lamb meat. Food safety, lamb breed and meat origin were the top three factors concerned when the respondents purchase lamb meat. >65.0% of the respondents from the selected valid questionnaires preferred to purchase traceable lamb meat. In conclusion, the development of technologies to maintain fresh lamb meat quality and traceable lamb meat products are required in China.
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http://dx.doi.org/10.1016/j.meatsci.2022.108878DOI Listing
October 2022

Inflammatory endothelium-targeted and cathepsin responsive nanoparticles are effective against atherosclerosis.

Theranostics 2022 16;12(9):4200-4220. Epub 2022 May 16.

Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.

Atherosclerosis is characterized by lipid accumulation, plaque formation, and artery stenosis. The pharmacological treatment is a promising therapy for atherosclerosis, but this approach faces major challenges such as targeted drug delivery, controlled release, and non-specific clearance. Based on the finding that the cathepsin k (CTSK) enzyme is enriched in atherosclerotic lesions, we constructed an integrin αβ targeted and CTSK-responsive nanoparticle to control the release of rapamycin (RAP) locally. The targeted and responsive nanoparticles (T/R NPs) were engineered by the self-assembly of a targeting polymer PLGA-PEG-c(RGDfC) and a CTSK-sensitive polymer PLGA-Pep-PEG. PLGA-Pep-PEG was also modified with a pair of FRET probe to monitor the hydrolysis events. Our results indicated that [email protected]/R NPs accelerated the release of RAP in response to CTSK stimulation , which significantly inhibited the phagocytosis of OxLDL and the release of cytokines by inflammatory macrophages. Additionally, T/R NPs had prolonged blood retention time and increased accumulation in the early and late stage of atherosclerosis lesions. [email protected]/R NPs significantly blocked the development of atherosclerosis and suppressed the systemic and local inflammation in ApoE mice. [email protected]/R NPs hold a great promise as a drug delivery system for safer and more efficient therapy of atherosclerosis.
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http://dx.doi.org/10.7150/thno.70896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169363PMC
May 2022

A Biomimetic Textile with Self-Assembled Hierarchical Porous Fibers for Thermal Insulation.

ACS Appl Mater Interfaces 2022 Jun 26;14(22):25851-25860. Epub 2022 May 26.

Applied Mechanics Laboratory, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, P. R. China.

Natural biomaterials with a porous structure inspired smart textiles for personal thermal management. Inspired by the hierarchically fibrous structure of hides, self-assembled hierarchical fibers with cross-scale porous networks are fabricated by the facile wet-spinning method. The biomimetic textile (abbreviated as "T") woven by such fibers exhibits a low thermal conductivity (0.07 W/mK) comparable to that of cowhide. It also shows a high mechanical strength of up to 10 MPa as well as good flexibility (fracture strain exceeds 300%) and hydrophobicity. The heat conduction mechanism of the hierarchical structure is analyzed via finite element simulation. When immersed with the phase-change material, the textile (named as "P") works like an adipose layer. Integration of the layers of T and P effectively slows down the heat conduction and decreases the surface temperature, resembling an animal insulation system. The study paves the way to mass production of high-performance biomimetic materials with hierarchical cellular microstructures for application in thermal insulation.
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http://dx.doi.org/10.1021/acsami.1c24367DOI Listing
June 2022

Highly efficient removal of glyphosate from water by hierarchical-pore UiO-66: Selectivity and effects of natural water particles.

J Environ Manage 2022 Aug 17;316:115301. Epub 2022 May 17.

School of Resources and Environment, Anhui Agricultural University, Hefei, 230036, PR China; Hefei Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, PR China. Electronic address:

The selective removal of glyphosate (GP) from aqueous environments is crucial for ensuring human health and environmental sustainability. The preparation of hierarchical-pore MOFs and the reasonable regulation of the pore size are effective strategies for achieving selective removal. In this study, we applied hierarchical-pore UiO-66 analogues (HUiO-66s) synthesized by the template technique through a mild method for the removal of GP from water. The results showed that the maximum adsorption capacity of HUiO-66s was as high as 400 mg/g, which is higher than that of most reported adsorbents. Notably, HUiO-66s showed the highest adsorption rate and distribution coefficient for GP in a multivariate system containing different organophosphorus pesticides and antibiotics, exhibiting suitable selective adsorption performance for GP. Furthermore, GP adsorption onto HUiO-66-2 (prepared from 2 mL of MOF-5 template) did not affect the presence of competing anions and humic acids. Naturally occurring particles in the water body had an enhanced (i.e., AlO, sepiolite, and montmorillonite), reduced (i.e., illite and SiO), or insignificant (i.e., kaolin) effect on the GP adsorption rate of HUiO-66-2. Further analysis based on the bulk adsorption results and microscopic characterisation indicated that the pore structure synergistically occurred with metal-ligand bonding, hydrogen bonding, and electrostatic interactions, which together determined the GP adsorption. Overall, the high adsorption and apparent adsorption selectivity of HUiO-66s facilitated the rapid separation and removal of GP in complex aqueous environments. Our findings provide insights into the transport and fate of MOFs and contaminants in natural aquatic systems.
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http://dx.doi.org/10.1016/j.jenvman.2022.115301DOI Listing
August 2022

Single-cell and spatial analysis reveal interaction of FAP fibroblasts and SPP1 macrophages in colorectal cancer.

Nat Commun 2022 04 1;13(1):1742. Epub 2022 Apr 1.

Shanghai Institute of Immunology, Department of Immunology and Microbiology, and the Ministry of Education Key Laboratory of Cell Death and Differentiation, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Colorectal cancer (CRC) is among the most common malignancies with limited treatments other than surgery. The tumor microenvironment (TME) profiling enables the discovery of potential therapeutic targets. Here, we profile 54,103 cells from tumor and adjacent tissues to characterize cellular composition and elucidate the potential origin and regulation of tumor-enriched cell types in CRC. We demonstrate that the tumor-specific FAP fibroblasts and SPP1 macrophages were positively correlated in 14 independent CRC cohorts containing 2550 samples and validate their close localization by immuno-fluorescent staining and spatial transcriptomics. This interaction might be regulated by chemerin, TGF-β, and interleukin-1, which would stimulate the formation of immune-excluded desmoplasic structure and limit the T cell infiltration. Furthermore, we find patients with high FAP or SPP1 expression achieved less therapeutic benefit from an anti-PD-L1 therapy cohort. Our results provide a potential therapeutic strategy by disrupting FAP fibroblasts and SPP1 macrophages interaction to improve immunotherapy.
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http://dx.doi.org/10.1038/s41467-022-29366-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976074PMC
April 2022

Fluid Shear Stress-Induced Exosomes from Liver Cancer Cells Promote Activation of Cancer-Associated Fibroblasts via IGF2-PI3K Axis.

Front Biosci (Landmark Ed) 2022 03;27(3):104

Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 610041 Chengdu, Sichuan, China.

Background: Cancer-associated fibroblasts (CAFs) are of considerable importance in tumor progression by interacting with the tumor microenvironment. However, the hidden mechanism explaining how tumor cells interact with CAFs in the tumor mechanical microenvironment remains largely unknown.

Methods: We highlighted exosomes as the mediator modulating the interaction between liver cancer cells and CAFs under mechanical conditions. The normal hepatic stellate cells LX2 were exposed to the medium or exosomes from the HepG2 cells with or without fluid shear stress subjection, and the CAFs activation markers were checked. To further explore the potential role of PI3K, which is active in liver fibrosis, the PI3K inhibitor was used.

Results: The specific markers of CAFs, FAP, and α-SMA, increased in LX2 with subjection to the fluid shear stress-induced exosomes from HepG2 cells. In turn, the enriched IGF2 in the exosomes activated the IGF2-PI3K signaling pathway in LX2 cells.

Conclusions: These findings reveal that fluid shear stress-induced liver cancer cells possess a stronger capacity to convert normal fibroblasts to CAFs than statically cultured liver cancer cells, and tumor-derived exosomes mediated the intercellular cross-talk between liver cancer cells and fibroblasts.
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http://dx.doi.org/10.31083/j.fbl2703104DOI Listing
March 2022

All-Ion Monitoring-Directed Low-Abundance Protein Quantification Reveals CALB2 as a Key Promoter in Hepatocellular Carcinoma Metastasis.

Anal Chem 2022 04 25;94(16):6102-6111. Epub 2022 Mar 25.

Zhang Dayu School of Chemistry, Dalian University of Technology, Dalian 116024, China.

Because of the wide abundance range of the proteome, achieving high-coverage quantification of low-abundance proteins is always a major challenge. In this study, a complete pipeline focused on all-ion monitoring (AIM) is first constructed with the concept of untargeted parallel-reaction monitoring, including the seamless connection of protein sample preparation, liquid chromatography mass spectrometry (LC-MS) acquisition, and algorithm development to enable the in-depth quantitative analysis of low-abundance proteins. This pipeline significantly improves the reproducibility and sensitivity of sample preparation and LC-MS acquisition for low-abundance proteins, enabling all the precursors ions fragmented and collected. Contributed by the advantages of the AIM method with all the target precursor acquisition by the data-dependent acquisition (DDA) approach, together with the ability of data-independent acquisition to fragment all precursor ions, the quantitative accuracy and precision of low-abundance proteins are greatly enhanced. As a proof of concept, this pipeline is employed to discover the key differential proteins in the mechanism of hepatocellular carcinoma (HCC) metastasis. On the basis of the superiority of AIM, an extremely low-abundance protein, CALB2, is proposed to promote HCC metastasis and . We also reveal that CALB2 activates the TRPV2-Ca-ERK1/2 signaling pathway to induce HCC cell metastasis. In summary, we provide a universal AIM pipeline for the high-coverage quantification of low-abundance functional proteins to seek novel insights into the mechanisms of cancer metastasis.
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http://dx.doi.org/10.1021/acs.analchem.1c03562DOI Listing
April 2022

Regulation of Expression Represents the Difference Between Direct and Indirect Shoot Regeneration.

Front Plant Sci 2022 4;13:850726. Epub 2022 Mar 4.

State Key Laboratory of Crop Biology, State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, College of Life Sciences, College of Forestry, Shandong Agricultural University, Taian, China.

Somatic cells of higher plants possess the remarkable ability to regenerate new individuals via reestablishing apical meristems. Reconstitution of shoot meristem is the vital process and is required for application of plant biotechnology. Under culture condition, shoot meristem can be formed directly or indirectly, depending on the absence or presence of callus as the intermediate status. However, the difference of regulatory mechanisms between the two regeneration types remains unknown. In this study, we established a bi-directional system in which shoots regenerated directly from lateral root primordia (LRP) and indirectly from hypocotyl-derived callus simultaneously. The results based on this system revealed that regulation of expression represents the difference between the two regeneration types in two aspects. Firstly, number of founder cells expressing is tightly associated with regeneration types. Relatively more founder cells gave rise to callus and produce larger meristem, whereas less founder cells produce LRP that regenerate smaller meristem. Secondly, non-CG DNA methylation specifically regulated transcription in LRP and promoted direct shoot regeneration, but had no influence on indirect regeneration. The results provide new insights for understanding the regulatory mechanisms of cell fate transition during organogenesis.
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http://dx.doi.org/10.3389/fpls.2022.850726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931721PMC
March 2022

Sleep Quality Is Associated With Severe Meibomian Gland Disruption in Dry Eye.

Front Med (Lausanne) 2022 17;9:812705. Epub 2022 Feb 17.

Eye Center, Affiliated Second Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Purpose: To investigate the association between sleep quality and meibomian gland dropout characteristics in dry eye patients.

Methods: This cross-sectional study involved 172 dry eye patients with no history of conditions or factors that could confound dry eye disease (DED) and/or meibomian gland dropout. Participants underwent a comprehensive anterior eye assessment. The validated Athens Insomnia Scale (AIS) and Pittsburgh Sleep Quality Index (PSQI) were used to assess sleep quality. The measured outcomes were dry eye symptoms via the Ocular Surface Disease Index (OSDI), tear breakup time (TBUT), corneal fluorescein staining, meibomian gland function, and extent of meibomian gland dropout.

Results: Of the dry eye participants, 34.9% had severe meibomian gland dropout (SMD) and 41.3% of the subjects had poor sleep quality. Patients with poor sleep quality had greater Meibomian gland dropout while the sleep AIS and PSQI scores were significantly correlated with Meibomian gland dropout ( = 0.495, < 0.001; = 0.24, = 0.002; respectively). SMD patients had worse scores on all components of the PSQI (all < 0.001, corrected for age and sex). Use of sleep medication, poor habitual sleep efficiency, and sleep disturbance were particularly prevalent in SMD patients as compared to Non-severe meibomian gland dropout (NSMD) patients. Multivariate logistic regression analysis revealed that sleep quality was eventually associated with female gender ( = 0.042), OSDI ( = 0.004), TBUT ( = 0.036), and Meibomian gland dropout score ( < 0.001).

Conclusion: It was found that greater meibomian gland dropout in poor sleep quality individuals is especially related to use of sleep medication, poor habitual sleep efficiency, and sleep disturbance. This finding suggests a need for long-term studies of anterior eye health in people with poor sleep quality.
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http://dx.doi.org/10.3389/fmed.2022.812705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8891226PMC
February 2022

Effects of Low-Dose Aspirin Combined with Vitamin E on the Incidence of Intrauterine Growth Restriction and Hemorheological Indexes of Pregnant Women in Patients with Gestational Hypertension.

Comput Math Methods Med 2022 21;2022:6328807. Epub 2022 Feb 21.

First People's Hospital of Linping District, Hangzhou 311100, China.

Objective: To investigate the effect of low-dose aspirin combined with vitamin E on the incidence of intrauterine growth restriction and hemorheological indexes of pregnant women in patients with gestational hypertension.

Method: 134 elderly patients with chronic urticaria treated in our hospital from November 2017 to November 2020 were studied. According to the treatment methods, they were randomly divided into observation and control groups. There were 67 patients in the observation group, aged 20-37 years, with an average of (25.7 ± 2.75) years. There were 67 patients in the control group, aged 21-35 years, with an average of (26.3 ± 3.17) years. No significant difference was observed between the two groups ( > 0.05).

Results: The number of cases with postpartum hemorrhage and intrauterine growth restriction in the observation group was less than that in the control group. The total incidence rate was lower than that in the control group. There were significant differences in the above results ( < 0.05). The number of patients with preterm birth in the observation group was less than that in the control group, but there was no significant difference in the results ( > 0.05). The head circumference, abdominal circumference, biparietal diameter, and femoral length diameter in the control and observation groups increased significantly after treatment ( < 0.05). Compared with the control group, the head circumference, abdominal circumference, biparietal diameter, and femoral diameter in the observation group increased more after treatment, and the results were statistically poor ( < 0.05). The systolic blood pressure, diastolic blood pressure, and mean arterial pressure in the control and observation groups decreased significantly after treatment, and the results were statistically different ( < 0.05). Compared with the control group, the systolic blood pressure, diastolic blood pressure, and mean arterial pressure in the observation group decreased more after treatment. The results were statistically different ( < 0.05). The plasma viscosity levels, whole blood high shear viscosity, and whole blood low shear viscosity in the control and observation groups decreased significantly after treatment, and the results were statistically different ( < 0.05). Compared with the control group, plasma viscosity levels, whole blood high shear viscosity, and whole blood low shear viscosity in the observation group decreased more after treatment, and the results were statistically different ( < 0.05). The control and observation groups' fetal systolic/diastolic pressure and pulsatile index decreased significantly after treatment, and the results were statistically different ( < 0.05). Compared with the control group, the fetal systolic/diastolic blood pressure and pulsatile index in the observation group decreased more after treatment, and the results were statistically poor ( < 0.05).

Conclusion: Low-dose aspirin combined with vitamin E is effective in treating intrauterine growth restriction in patients with gestational hypertension. It can effectively control the blood pressure and blood flow of patients and newborns and improve pregnancy outcomes without increasing the incidence of adverse reactions. It is worthy of clinical promotion.
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http://dx.doi.org/10.1155/2022/6328807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885198PMC
March 2022

Promotion of Ros-mediated Bax/Cyt-c apoptosis by polyphyllin II leads to suppress growth and aggression of glioma cells.

Transl Cancer Res 2021 Sep;10(9):3894-3905

Department of Chinese Materia Medica and Natural Medicines, Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, School of Pharmacy, Air Force Medical University, Xi'an, China.

Background: Gliomas remain among the most difficult cancers to treat, with a 5-year overall survival no greater than 5%. Many saponins showed a wide spectrum of anti-cancer activities at low concentration. Polyphyllin II is one of the common saponins from Paris polyphylla. However, the effect of Polyphyllin II on glioma cells has not been evaluated. Objective of the present study was to investigate whether Polyphyllin II have inhibition on glioma cells, and the possible mechanisms.

Methods: The viability of U87 and U251 cells was detected by cell counting kit-8, cell counting real time cellular analysis and cell clone formation methods. Transwell was used to estimate the aggression of U87 and U251. The cell apoptosis rate was tested by flow cytometry. The morphological change was determined by transmission electron microscopy. The levels of AKT, phosphorylation of AKT, Bax, Bcl-2, cytochrome c, and cleaved caspase 3 proteins were assessed by Western blot. N-acetyl-L-cysteine was used to check the role of ROS in polyphyllin II inhibition to glioma cells.

Results: Polyphyllin II showed significant suppress to proliferation and aggression of U87 and U251 in a dose- and time- dependent manner. Result of flow cytometry confirmed that Polyphyllin II induced apoptosis to U87 and U251 cells. Transmission electron microscopy observation revealed majority of the glioma cells treated with Polyphyllin II had turgidity of mitochondrion, disarrangement, diminution and vacuolization, those refer to mitochondrial apoptosis. Western blot indicated that Polyphyllin II promoted cyt-c, Bax, caspase 3 and cleaved-caspase 3, and decreased Bcl-2, AKT and p-AKT. Rescue experiments using N-acetyl-L-cysteine, a reactive oxygen species scavenger, reversed the levels of Bax and cyt-c, and the inhibition in Polyphyllin II-treated U87 and U251 cells.

Conclusions: The present findings revealed that polyphyllin II may be a potential drug against glioma.
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http://dx.doi.org/10.21037/tcr-21-966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797898PMC
September 2021

Mechanically Competent Chitosan-Based Bioadhesive for Tendon-to-Bone Repair.

Adv Healthc Mater 2022 05 22;11(10):e2102344. Epub 2022 Jan 22.

Department of Orthopedic Surgery, Columbia University, New York, NY, 10032, USA.

Current suture-based surgical techniques used to repair torn rotator cuff tendons do not result in mechanically competent tendon-to-bone attachments, leading to high postoperative failure rates. Although adhesives have been proposed to protect against sutures tearing through tendon during healing, no currently available adhesive meets the clinical needs of adhesive strength, biocompatibility, and promotion of healing. Here, a biocompatible, graded, 3,4-dihydroxy phenyl chitosan (BGC) bioadhesive designed to meet these needs is presented. Although 3,4-dihydroxy phenyl chitosan (DP-chitosan) bioadhesives are biocompatible, their adhesion strength is low; soluble oxidants or cross-linking agents can be added for higher bonding strength, but this sacrifices biocompatibility. These challenges are overcome by developing a periodate-modified ion exchange resin-bead filtration system that oxidizes catechol moieties to quinones and filters off the activating agent and resin. The resulting BGC bioadhesive exhibited sixfold higher strength compared to commercially available tissue adhesives, with strength in the range necessary to improve tendon-to-bone repair (≈1MPa, ≈20% of current suture repair strength). The bioadhesive is biocompatible and promoted tenogenesis; cells exposed to the bioadhesive demonstrated enhanced expression of collagen I and the tenogenic marker Scx. Results demonstrated that the bioadhesive has the potential to improve the strength of a tendon-to-bone repair and promote healing.
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http://dx.doi.org/10.1002/adhm.202102344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117437PMC
May 2022

Comprehensive structure and functional adaptations of the yeast nuclear pore complex.

Cell 2022 01 3;185(2):361-378.e25. Epub 2022 Jan 3.

Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA. Electronic address:

Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport.
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http://dx.doi.org/10.1016/j.cell.2021.12.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928745PMC
January 2022

CN-3 increases TMZ sensitivity and induces ROS-dependent apoptosis and autophagy in TMZ-resistance glioblastoma.

J Biochem Mol Toxicol 2022 Mar 29;36(3):e22973. Epub 2021 Dec 29.

Department of Neurosurgery, Xijing Institute of Clinical Neuroscience, Air Force Medical University, Xi'an, China.

Many glioma patients develop resistance to temozolomide (TMZ) treatment, resulting in reduced efficacy and survival rates. TMZ-resistant cell lines SHG44R and U87R, which highly express O -methylguanine DNA methyltransferase (MGMT) and P-gp, were established. CN-3, a new asterosaponin, showed cytotoxic effects on TMZ-resistant cells in a dose- and time-dependent manner via reactive oxygen species (ROS)-mediated apoptosis and autophagy. Transmission electron microscopy and monodansylcadaverine (MDC) staining showed turgidity of the mitochondria and autophagosomes in CN-3-treated SHG44R and U87R cells. The autophagy inhibitor 3-methyladenine was used to confirm the important role of autophagy in CN-3 cytotoxicity in TMZ-resistant cells. The ROS scavenger N-acetyl- l-cysteine (NAC) attenuated the levels of ROS induced by CN-3 and, therefore, rescued the CN-3 cytotoxic effect on the viability of SHG44R and U87R cells by Cell Counting Kit-8 assays and JuLI-Stage videos. MDC staining also confirmed that NAC rescued an autophagosome increase in CN-3-treated SHG44R and U87R cells. Western blotting revealed that CN-3 increased Bax, cleaved-caspase 3, cytochrome C, PARP-1, LC3-Ⅱ, and Beclin1, and decreased P-AKT, Bcl-2, and p62. Further rescue experiments revealed that CN-3 induced apoptosis and autophagy through ROS-mediated cytochrome C, cleaved-caspase 3, Bcl-2, P-AKT, PARP-1, and LC3-Ⅱ. In addition, CN-3 promoted SHG44R and U87R cells sensitive to TMZ by reducing the expression of P-gp, MGMT, and nuclear factor kappa B p65, and it had a synergistic cytotoxic effect with TMZ. Moreover, CN-3 disrupted the natural cycle arrest and inhibited the migration of SHG44R and U87R cells by promoting cyclin E1 and D1, and by decreasing P21, P27, N-cadherin, β-catenin, transforming growth factor beta 1, and Smad2.
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http://dx.doi.org/10.1002/jbt.22973DOI Listing
March 2022

Hedgehog signaling underlying tendon and enthesis development and pathology.

Matrix Biol 2022 01 24;105:87-103. Epub 2021 Dec 24.

Department of Orthopedic Surgery, Columbia University, Black Building, Room 1408, 650W 168 ST, New York, NY 10032-3702, United States; Department of Biomedical Engineering, Columbia University, New York, NY, United States. Electronic address:

Hedgehog (Hh) signaling has been widely acknowledged to play essential roles in many developmental processes, including endochondral ossification and growth plate maintenance. Furthermore, a rising number of studies have shown that Hh signaling is necessary for tendon enthesis development. Specifically, the well-tuned regulation of Hh signaling during development drives the formation of a mineral gradient across the tendon enthesis fibrocartilage. However, aberrant Hh signaling can also lead to pathologic heterotopic ossification in tendon or osteophyte formation at the enthesis. Therefore, the therapeutic potential of Hh signaling modulation for treating tendon and enthesis diseases remains uncertain. For example, increased Hh signaling may enhance tendon-to-bone healing by promoting the formation of mineralized fibrocartilage at the healing interface, but pathologic heterotopic ossification may also be triggered in the adjacent tendon. Further work is needed to elucidate the distinct functions of Hh signaling in the tendon and enthesis to support the development of therapies that target the pathway.
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http://dx.doi.org/10.1016/j.matbio.2021.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821161PMC
January 2022

Which Type of Pincer Complex Is Thermodynamically More Stable? Understanding the Structures and Relative Bond Strengths of Group 10 Metal Complexes Supported by Benzene-Based PYCYP Pincer Ligands.

Inorg Chem 2021 Dec 8;60(24):18924-18937. Epub 2021 Dec 8.

Henan Key Laboratory of Boron Chemistry and Advanced Energy Materials, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Centre of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, China.

The influence of the pincer platform composition and substitution on the reactivity and physical properties of pincer complexes can be easily explored through different experimental techniques. However, the influence of these factors on the molecular structures and thermodynamic stability of pincer complexes is usually very subtle and cannot always be unambiguously established. To rationalize this subtle influence, a survey of crystallographic data from 130 group 10 metal pincer complexes supported by benzene-based PYCYP pincer ligands, [2,6-(RPY)CHR']MX (Y = CH, NH, O, S; M = Ni, Pd, Pt; R = Bu, Pr, Ph, Cy, Me; R' = COMe, Bu, CF, Ac; = 0-2; X = F, Cl, Br, I, H, SH, SPh, SBn, Ph, Me, N, NCS), was carried out. Theoretical calculations for some selected complexes were performed to evaluate the relative bond strength. It was found that the M-C bond length decreases following the linker series of CH > NH > O and that the relative M-C bond strength increases following the linker series of CH < NH < O. In most cases, the M-P bond length decreases following the linker series of NH > CH > O. The relative M-P bond strength increases following the linker series of CH < NH < O. A comparison of the thermochemical balance for the isodesmic displacement of the side-arm interactions with PH as a probe ligand indicated that the Ni-P bond in a PCCCP-type pincer complex is far less difficult to break compared with that in a POCOP-type complex. As a result, with the same donor substituents and the same auxiliary ligand, the POCOP-type pincer complexes are thermodynamically more stable than the PCCCP complexes. The influence of other backbone and donor substitutions as well as the pincer platform composition on the M-C, M-P, and M-X bond lengths, relative bond strengths, and P-M-P bite angles was also discussed.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02722DOI Listing
December 2021

Impact of Chilling Rate on the Evolution of Volatile and Non-Volatile Compounds in Raw Lamb Meat during Refrigeration.

Foods 2021 Nov 12;10(11). Epub 2021 Nov 12.

Key Laboratory of Agro-Products Processing, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

The aim of this study was to investigate the effect of chilling rate (1.44, 22.2, and 32.4 °C/h) on the evolution of volatile and non-volatile compounds in raw lamb meat during refrigeration (1, 24, 72, and 120 h). Through orthogonal projection to latent structure-discriminant analysis, the calculation of odor activity values (OAV > 1) and taste activity values (TAV > 1) analysis, 1-octen-3-ol, (E, E)-2,4-decadienal, nonanal, hexanal, nona-3,5-dien-2-one, 2,3-octanedione, hexanoic acid, 1-nonen-4-ol, aspartate (Asp), Glutamic Acid (Glu), 5'-GMP, 5'-IMP, and 5'-AMP were regarded as differential flavor or taste compounds for raw meat undergone different chilling rates. With a rapid chilling rate at 24 h after slaughter, the contribution of 1-octen-3-ol decreased, but (E, E)-2,4-decadienal increased. Moreover, at 24 h post-mortem, the equivalent umami concentration of Asp, Glu, 5'-GMP, 5'-IMP and 5'-AMP in raw meat were significantly lower at a chilling rate of 1.44 °C/h than 32.4 °C/h ( < 0.05). Conclusively, under the rapid chilling rate, more fatty odor and umami compounds accumulated in 24 h aged meat.
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http://dx.doi.org/10.3390/foods10112792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620043PMC
November 2021

Transient neonatal shoulder paralysis causes early osteoarthritis in a mouse model.

J Orthop Res 2021 Nov 23. Epub 2021 Nov 23.

Department of Orthopedic Surgery, Columbia University, New York, New York, USA.

Neonatal brachial plexus palsy (NBPP) occurs in approximately 1.5 of every 1,000 live births. The majority of children with NBPP recover function of the shoulder. However, the long-term risk of osteoarthritis (OA) in this population is unknown. The purpose of this study was to investigate the development of OA in a mouse model of transient neonatal shoulder paralysis. Neonatal mice were injected twice per week for 4 weeks with saline in the right supraspinatus muscle (Saline, control) and botulinum toxin A (BtxA, transient paralysis) in the left supraspinatus muscle, and then allowed to recover for 20 or 36 weeks. Control mice received no injections, and all mice were sacrificed at 24 or 40 weeks. BtxA mice exhibited abnormalities in gait compared to controls through 10 weeks of age, but these differences did not persist into adulthood. BtxA shoulders had decreased bone volume (-9%) and abnormal trabecular microstructure compared to controls. Histomorphometry analysis demonstrated that BtxA shoulders had higher murine shoulder arthritis scale scores (+30%), and therefore more shoulder OA compared to controls. Articular cartilage of BtxA shoulders demonstrated stiffening of the tissue. Compared with controls, articular cartilage from BtxA shoulders had 2-fold and 10-fold decreases in Dkk1 and BMP2 expression, respectively, and 3-fold and 14-fold increases in Col10A1 and BGLAP expression, respectively, consistent with established models of OA. In summary, a brief period of paralysis of the neonatal mouse shoulder was sufficient to generate early signs of OA in adult cartilage and bone.
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http://dx.doi.org/10.1002/jor.25225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124737PMC
November 2021

Characterization and Discrimination of Key Aroma Compounds in Pre- and Postrigor Roasted Mutton by GC-O-MS, GC E-Nose and Aroma Recombination Experiments.

Foods 2021 Oct 8;10(10). Epub 2021 Oct 8.

Key Laboratory of Agro-Products Processing, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

The key aroma compounds in the pre- and postrigor roasted mutton were studied in this study. The results showed that 33 and 30 odorants were detected in the pre- and postrigor roasted mutton, respectively. Eight aroma compounds, including 3-methylbutanal, pentanal, hexanal, heptanal, octanal, nonanal, 1-octen-3-ol, and 2-pentylfuran, were confirmed as key odorants by aroma recombination and omission experiments. The aroma profiles of pre- and postrigor roasted mutton both presented great fatty, roasty, meaty, grassy, and sweet odors. Particularly, the concentrations of hexanal, heptanal, octanal, nonanal, 1-octen-3-ol, and 2-pentylfuran in postrigor roasted mutton were significantly higher ( < 0.05) than those in the prerigor roasted mutton. The postrigor was more suitable for roasting than the prerigor . The pre- and postrigor roasted mutton could be obviously discriminated based on the aroma compounds by orthogonal partial least squares discrimination analysis (OPLS-DA) and principal component analysis (PCA). Hexanal and 1-octen-3-ol might potential markers for the discrimination of the pre- and postrigor roasted mutton.
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http://dx.doi.org/10.3390/foods10102387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535600PMC
October 2021

β-Lapachone Selectively Kills Hepatocellular Carcinoma Cells by Targeting NQO1 to Induce Extensive DNA Damage and PARP1 Hyperactivation.

Front Oncol 2021 5;11:747282. Epub 2021 Oct 5.

Department of Radiation Oncology, Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, IN, United States.

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death globally. Currently there is a lack of tumor-selective and efficacious therapies for hepatocellular carcinoma. β-Lapachone (ARQ761 in clinical form) selectively kill NADPH: quinone oxidoreductase 1 (NQO1)-overexpressing cancer cells. However, the effect of β-Lapachone on HCC is virtually unknown. In this study, we found that relatively high NQO1 and low catalase levels were observed in both clinical specimens collected from HCC patients and HCC tumors from the TCGA database. β-Lapachone treatment induced NQO1-selective killing of HCC cells and caused ROS formation and PARP1 hyperactivation, resulting in a significant decrease in NAD and ATP levels and a dramatic increase in double-strand break (DSB) lesions over time . Administration of β-Lapachone significantly inhibited tumor growth and prolonged survival in a mouse xenograft model . Our data suggest that NQO1 is an ideal potential biomarker, and relatively high ratios in HCC tumors but low ratios in normal tissues offer an optimal therapeutic window to use β-Lapachone. This study provides novel preclinical evidence for β-Lapachone as a new promising chemotherapeutic agent for use in NQO1-positive HCC patients.
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http://dx.doi.org/10.3389/fonc.2021.747282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523939PMC
October 2021

The formation of key aroma compounds in roasted mutton during the traditional charcoal process.

Meat Sci 2022 Feb 2;184:108689. Epub 2021 Oct 2.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China. Electronic address:

The formation of key aroma compounds in roasted mutton during the traditional charcoal process were investigated. The results indicated that the samples roasted for 0-15 min could be discriminated using a flash GC E-nose and GC-O-MS combined with multivariate data analysis. A total of 37 odorants were identified, among which 15 odorants were confirmed as key aroma compounds by aroma recombination experiments. Significant increases in key aroma compositions and concentrations in samples were observed during the roasting process, in which hexanal had the highest concentration. The odour activity values (OAVs) of 15 key aroma compounds were maintained at high levels in the samples after roasting for 10 min. The roasted mutton had typical aromas of meaty, fatty, roasty, grassy, and sweet odours. The multivariate linear modeling indicated that a lower specific heat capacity and lower water activity could contribute to the formation of aroma compounds of samples.
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http://dx.doi.org/10.1016/j.meatsci.2021.108689DOI Listing
February 2022

Effect of Postmortem Phases on Lamb Meat Quality: A Physicochemical, Microstructural and Water Mobility Approach.

Food Sci Anim Resour 2021 Sep 1;41(5):802-815. Epub 2021 Sep 1.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

To investigate the effect of postmortem phases on lamb meat quality, the physicochemical quality, microstructure and water mobility of oyster cut, short loin, knuckle and silverside muscles from Small-Tail Han sheep were evaluated in the pre-rigor, rigor mortis and post-rigor phases. Pre-rigor lamb meat had higher pH and water holding capacity (WHC), whereas lower CIE L*, b*, hue angle values than rigor mortis and post-rigor meat (p<0.05). The Warner-Bratzler shear force (WBSF) values were higher in rigor mortis short loin and silverside than their pre-rigor and post-rigor counterparts, pre-rigor short loin had lower WBSF value than its post-rigor counterpart (p<0.05). Muscle fibers shrank laterally and longitudinally during the onset of rigor mortis. Rigor mortis and post-rigor lamb meat exhibited wide I-bands, dark A-bands, short sarcomeres and large inter-myofibrillar spaces. The shift of immobilized water to free water and repulsion from the intra-myofibrillar space to the extracellular space result in the increase of water loss in rigor mortis and post-rigor lamb meat. The results of the principal component analysis (PCA) indicated that rigor mortis and post-rigor lamb meat had similar quality properties but different from pre-rigor lamb meat. In conclusion, the lamb meat in the pre-rigor phase had good tenderness, color and WHC. The results of this research could provide some theoretical references for lamb meat production and processing.
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http://dx.doi.org/10.5851/kosfa.2021.e37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460324PMC
September 2021

Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation.

Sci Signal 2021 Oct 5;14(703):eabc5944. Epub 2021 Oct 5.

Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.

The parathyroid hormone (PTH) type 1 receptor (PTHR) is a class B G protein–coupled receptor (GPCR) that regulates mineral ion, vitamin D, and bone homeostasis. Activation of the PTHR by PTH induces both transient cell surface and sustained endosomal cAMP production. To address whether the spatial (location) or temporal (duration) dimension of PTHR-induced cAMP encodes distinct biological outcomes, we engineered a biased PTHR ligand (PTH) that elicits cAMP production at the plasma membrane but not at endosomes. PTH stabilized a unique active PTHR conformation that mediated sustained cAMP signaling at the plasma membrane due to impaired β-arrestin coupling to the receptor. Experiments in cells and mice revealed that sustained cAMP production by cell surface PTHR failed to mimic the pharmacological effects of sustained endosomal cAMP production on the abundance of the rate-limiting hydroxylase catalyzing the formation of active vitamin D, as well as increases in circulating active vitamin D and Ca and in bone formation in mice. Thus, similar amounts of cAMP generated by PTHR for similar lengths of time in different cellular locations, plasma membrane and endosomes, mediate distinct physiological responses. These results unveil subcellular signaling location as a means to achieve specificity in PTHR-mediated biological outcomes and raise the prospect of rational drug design based upon spatiotemporal manipulation of GPCR signaling.
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http://dx.doi.org/10.1126/scisignal.abc5944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682804PMC
October 2021

Rapid Nondestructive Simultaneous Detection for Physicochemical Properties of Different Types of Sheep Meat Cut Using Portable Vis/NIR Reflectance Spectroscopy System.

Foods 2021 Aug 24;10(9). Epub 2021 Aug 24.

Chinese Academy of Agricultural Sciences, National Risk Assesment Laboratory of Agro-Products Processing Quality and Safety, Institute of Food Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

The visible and near-infrared spectroscopy (Vis/NIRS) models for sheep meat quality evaluation using only one type of meat cut are not suitable for other types. In this study, a novel portable Vis/NIRS system was used to simultaneously detect physicochemical properties (pH, color *, *, *, cooking loss, and shear force) for different types of sheep meat cut, including silverside, back strap, oyster, fillet, thick flank, and tenderloin cuts. The results show that the predictive abilities for all parameters could be effectively improved by spectral preprocessing. The coefficient of determination () and residual predictive deviation (RPD) of the optimal prediction models for pH, *, *, *, cooking loss, and shear force were 0.79 and 3.50, 0.78 and 2.28, 0.68 and 2.46, 0.75 and 2.62, 0.77 and 2.19, and 0.83 and 2.81, respectively. The findings demonstrate that Vis/NIR spectroscopy is a useful tool for predicting the physicochemical properties of different types of meat cut.
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http://dx.doi.org/10.3390/foods10091975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468935PMC
August 2021

Tailored metal-organic frameworks facilitate the simultaneously high-efficient sorption of UO and ReO in water.

Sci Total Environ 2021 Dec 4;799:149468. Epub 2021 Aug 4.

School of Resources and Environment, Anhui Agricultural University, Hefei 230036, PR China; Hefei Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, PR China. Electronic address:

The simultaneously efficient extraction of radioactive metal cations and anions from radioactive waste is of great interest for the proper disposal of spent fuel and environmental protection. Modifying metal-organic frameworks (MOFs) into multifunctional materials with controllable and desired properties is an efficient strategy for broadening their practical applications. Herein, poly(ethyleneimine) (PEI) tailored MIL-101(Cr) (MILP) was obtained through an easy operation and low-cost strategy and was utilized to simultaneously extract uranium (UO) and rhenium (ReO) from water. The effects of PEI coating amounts, system pH, contact time, initial UO/ReO concentrations, ionic strength, as well as interfering ions were studied to evaluate the sorption performance of MILP composites. The maximum sorption capacity was 416.67 mg/g for UO at pH 5.5 and 434.78 mg/g for ReO at pH 3.5, levels that are superior to those of most adsorbents. The sorption of UO/ReO occurred in a pH-dependent, spontaneous and endothermic manner, which showed preferable modeling by the pseudo-second-order (PSO) kinetic equation and Freundlich isotherm equation. The adsorption of ReO was inhibited by the coexistence of UO and high ion strength. Batch experiments and X-ray photoelectron spectroscopy (XPS) results indicate that UO/ReO sorption was driven by the abundant amino groups and unsaturated metal sites in the MILP-3 composites. MILP-3 also showed excellent recycling performance and maintained high sorption capacities for UO/ReO in different simulated water samples. This study shows that MILP composites can effectively extract radioactive metal cations and anions from water, and lays a foundation for designing an excellent new category of candidates with versatile functions for wastewater management.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149468DOI Listing
December 2021

Fluid shear stress activates YAP to promote epithelial-mesenchymal transition in hepatocellular carcinoma.

Mol Oncol 2021 11 2;15(11):3164-3183. Epub 2021 Aug 2.

Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.

Epithelial-mesenchymal transition (EMT) mediated by fluid shear stress (FSS) in the tumor microenvironment plays an important role in driving metastasis of the malignant tumor. As a mechanotransducer, Yes-associated protein (YAP) is known to translocate into the nucleus to initiate transcription of genes involved in cell proliferation upon extracellular biophysical stimuli. Here, we showed that FSS facilitated cytoskeleton rearrangement in hepatocellular carcinoma cells, which led to the release of YAP from its binding partner, integrin β subunit, in the cytomembrane. Moreover, we found that upregulation of guanine nucleotide exchange factor (GEF)-H1, a microtubule-associated Rho GEF, is a critical step in the FSS-induced translocation of YAP. Nuclear YAP activated the expression of the EMT-regulating transcription factor SNAI1, but suppressed the expression of N6-methyladenosine (m A) modulators; together, this promoted the expression of EMT-related genes. We also observed that FSS-treated HepG2 cells showed markedly increased tumorigenesis and metastasis in vivo. Collectively, our findings unravel the underlying molecular processes by which FSS induces translocation of YAP from the cytomembrane to the nucleus, contributes to EMT and enhances metastasis in hepatocellular carcinoma.
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http://dx.doi.org/10.1002/1878-0261.13061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564657PMC
November 2021
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