Publications by authors named "Fei Cheng"

200 Publications

A Pedigree Analysis and Clonal Correlations of the Coexistence of B-Cell Lymphoma and Histiocytic/Dendritic Cell Tumor.

Int J Surg Pathol 2021 May 3:10668969211013402. Epub 2021 May 3.

71069The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Histiocytic/dendritic cell tumors are rare in clinical practice. It is postulated that they originate from bone marrow stem cells. Accumulating evidence has established the existence of immunoglobulin gene and T-cell receptor gene rearrangements in these tumors. Cases of transdifferentiation across lineages from follicular lymphoma to histiocytic/dendritic cell tumors have also been reported. Herein, we report 2 adult males with histiocytic neoplasms coexisting with B-cell lymphoma. Laser capture microdissection and capillary electrophoresis polymerase chain reaction analysis revealed comparable immunoglobulin gene rearrangement in both patients. In one case, chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), Langerhans cell sarcoma, and histiocytic sarcoma coexisted in the lymph nodes. 11q22 deletion often present in CLL/SLL and expression of the gene was detected in all the 3 components. In the other case, there diffuse large B-cell lymphoma and histiocytic sarcoma coexisted in the spleen. Forty-seven mutated genes commonly found in B-cell lymphoma were detected by next-generation sequencing. In the same line, , , , , and genes were found to have similar mutation sites. The results of this study will contribute in providing new ideas for targeted treatment of these diseases.
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http://dx.doi.org/10.1177/10668969211013402DOI Listing
May 2021

Metaform optics: Bridging nanophotonics and freeform optics.

Sci Adv 2021 Apr 30;7(18). Epub 2021 Apr 30.

The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.

The demand for high-resolution optical systems with a compact form factor, such as augmented reality displays, sensors, and mobile cameras, requires creating new optical component architectures. Advances in the design and fabrication of freeform optics and metasurfaces make them potential solutions to address the previous needs. Here, we introduce the concept of a metaform-an optical surface that integrates the combined benefits of a freeform optic and a metasurface into a single optical component. We experimentally realized a miniature imager using a metaform mirror. The mirror is fabricated via an enhanced electron beam lithography process on a freeform substrate. The design degrees of freedom enabled by a metaform will support a new generation of optical systems.
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http://dx.doi.org/10.1126/sciadv.abe5112DOI Listing
April 2021

Predicting rifampicin resistance mutations in bacterial RNA polymerase subunit beta based on majority consensus.

BMC Bioinformatics 2021 Apr 22;22(1):210. Epub 2021 Apr 22.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 511443, China.

Background: Mutations in an enzyme target are one of the most common mechanisms whereby antibiotic resistance arises. Identification of the resistance mutations in bacteria is essential for understanding the structural basis of antibiotic resistance and design of new drugs. However, the traditionally used experimental approaches to identify resistance mutations were usually labor-intensive and costly.

Results: We present a machine learning (ML)-based classifier for predicting rifampicin (Rif) resistance mutations in bacterial RNA Polymerase subunit β (RpoB). A total of 186 mutations were gathered from the literature for developing the classifier, using 80% of the data as the training set and the rest as the test set. The features of the mutated RpoB and their binding energies with Rif were calculated through computational methods, and used as the mutation attributes for modeling. Classifiers based on five ML algorithms, i.e. decision tree, k nearest neighbors, naïve Bayes, probabilistic neural network and support vector machine, were first built, and a majority consensus (MC) approach was then used to obtain a new classifier based on the classifications of the five individual ML algorithms. The MC classifier comprehensively improved the predictive performance, with accuracy, F-measure and AUC of 0.78, 0.83 and 0.81for training set whilst 0.84, 0.87 and 0.83 for test set, respectively.

Conclusion: The MC classifier provides an alternative methodology for rapid identification of resistance mutations in bacteria, which may help with early detection of antibiotic resistance and new drug discovery.
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http://dx.doi.org/10.1186/s12859-021-04137-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063314PMC
April 2021

A SnO Quantum Dots Embedded Carbon Nanocage Network with Ultrahigh Li Storage Capacity.

ACS Nano 2021 Apr 14;15(4):7021-7031. Epub 2021 Apr 14.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Linggong Road 2, Ganjingzi District, Dalian 116024, P. R. China.

Tin-based materials with high specific capacity have been studied as high-performance anodes for energy storage devices. Herein, a SnO ( = 0, 1, 2) quantum dots@carbon hybrid is designed and prepared by a binary oxide-induced surface-targeted coating of ZIF-8 followed by pyrolysis approach, in which SnO quantum dots (under 5 nm) are dispersed uniformly throughout the nitrogen-containing carbon nanocage. Each nanocage is cross-linked to form a highly conductive framework. The resulting SnO@C hybrid exhibits a large BET surface area of 598 m g, high electrical conductivity, and excellent ion diffusion rate. When applied to LIBs, the SnO@C reveals an ultrahigh reversible capacity of 1824 mAh g at a current density of 0.2 A g, and superior capacities of 1408 and 850 mAh g even at high rates of 2 and 5 A g, respectively. The full cell assembled using LiFePO as cathode exhibits the high energy density and power density of 335 Wh kg and 575 W kg at 1 C based on the total active mass of cathode and anode. Combined with XRD analysis, the superior electrochemical performance can be attributed to the SnO-ZnO-C asynchronous and united lithium storage mechanism, which is formed by the well-designed multifeatured construction composed of SnO quantum dots, interconnected carbon network, and uniformly dispersed ZnO nanoparticles. Importantly, this designed synthesis can be extended for the fabrication of other electrode materials by simply changing the binary oxide precursor to obtain the desired active component or modulating the type of MOFs coating to achieve high-performance LIBs.
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http://dx.doi.org/10.1021/acsnano.1c00088DOI Listing
April 2021

MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression.

Open Life Sci 2020 22;15(1):939-950. Epub 2020 Dec 22.

Department of Cardiovascular Medicine and Dongguan Cardiovascular Institute, The Third People's Hospital of Dongguan City, No. 1, Xianglong Road, Shi Long Town, Dongguan, China.

Diabetic nephropathy (DN) is the most serious long-term microvascular complication of diabetes, which mainly causes podocyte injury. Many studies have shown that microRNAs play a vital role in the development of DN. Studies have shown that miR-203-3p is involved in mesangial cell proliferation and apoptosis of DN mice. Therefore, we speculated that miR-203-3p might be related to the development of DN, but our study does not provide any evidence. In animal experiments, diabetic mice (db/db) were transfected with iR-203-3p overexpression lentiviral vectors (LV-miR-203-3p) and their control (LV-miR-con), with normal mice (db/m) being used as the control. High glucose (HG)-induced podocytes were used to construct a DN cell model . The expression levels of miR-203-3p, Semaphorin 3A (Sema3A) and inflammatory cytokines were detected by quantitative real-time polymerase chain reaction. Also, serum creatinine and blood urea nitrogen levels were used to evaluate the degree of renal injury in DN mice. Sema3A and apoptosis-related protein levels were assessed by the western blot analysis. Enzyme-linked immunosorbent assay was used to determine the different oxidative stress-related indicators and inflammatory cytokines. Flow cytometry and caspase-3 activity detection were used to analyze the degree of podocyte apoptosis. Our results suggested that the expression of miR-203-3p was lower in DN mice and in HG-induced podocytes. Overexpression of miR-203-3p reduced the body weight, blood glucose and renal injury of DN mice , as well as relieve the oxidative stress, inflammatory response and apoptosis of HG-induced podocytes . Functionally, Sema3A was a target of miR-203-3p, and Sema3A overexpression reversed the inhibitory effect of miR-203-3p on HG-induced podocyte injury. Our findings revealed that miR-203-3p alleviated the podocyte injury induced by HG via regulating Sema3A expression, suggesting that miR-203-3p might be a new therapeutic target to improve the progression of DN.
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http://dx.doi.org/10.1515/biol-2020-0088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874591PMC
December 2020

Rapamycin Inhibits Glioma Cells Growth and Promotes Autophagy by miR-26a-5p/DAPK1 Axis.

Cancer Manag Res 2021 22;13:2691-2700. Epub 2021 Mar 22.

Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Background: Glioma is a common intracranial malignant tumor with high rates of invasiveness and mortality. This study aimed to investigate the mechanism of rapamycin in glioma.

Methods: U118-MG cells were treated with and without rapamycin in vivo and then collected for RNA sequencing. Differentially expressed miRNAs (DEMs) were screened and verified. MiR-26a-5p was selected for functional verification, and the target gene of miR-26a-5p was identified. The effects of miR-26a-5p on cell proliferation, cell cycle, apoptosis, and autophagy were also investigated.

Results: In total, 58 up-regulated and 41 down-regulated DEMs were identified between rapamycin-treated and untreated U118-MG cells. MiR-26-5p levels were up-regulated in U118-MG cells treated with 12.5 μM rapamycin, and death-associated protein kinase 1 (DAPK1) expression, a direct miR-26a-5p target gene, was down-regulated. Rapamycin substantially inhibited cell proliferation and cell percentage in the S phase and promoted cell apoptosis; miR-26a-5p inhibitor increased cell proliferation and cell cycle and decreased cell apoptosis; DAPK1 overexpression further induced cell proliferation, increased the cell number in the S phase, and inhibited apoptosis in glioma cells. Notably, rapamycin increased the autophagy-related Beclin1 protein expression levels and the LC3 II/I ratio.

Conclusion: Rapamycin exerts anti-tumor effects by promoting autophagy in glioma cells, which was dependent on the miR-26a-5p/DAPK1 pathway activation by rapamycin.
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http://dx.doi.org/10.2147/CMAR.S298468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997605PMC
March 2021

Prognostic Value of S100P Expression in Patients With Digestive System Cancers: A Meta-Analysis.

Front Oncol 2021 5;11:593728. Epub 2021 Mar 5.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Digestive system cancers (DSCs) are associated with high morbidity and mortality. S100P has been reported as a prognostic biomarker in DSCs, but its prognostic value remains controversial. Accordingly, we conducted a meta-analysis to investigate whether S100P is correlated with overall survival (OS) of patients with DSCs. The relationship between S100P and clinicopathological features was also evaluated.

Methods: We systematically searched PubMed, Embase, Web of Science and Cochrane Library for eligible studies up to January 2020. In total, 16 publications with 1,925 patients were included.

Results: S100P overexpression was associated with poor OS of patient with DSCs (HR=1.54, 95% CI: 1.14-2.08, P=0.005). When stratified by anatomic structure, S100P overexpression was associated with poor prognosis in non-gastrointestinal tract cancers (HR=1.98, 95% CI: 1.44-2.72, P<0.001) but not in gastrointestinal tract cancers (HR=1.09, 95% CI: 0.66-1.81, P=0.727). When stratified by tumor type, S100P overexpression predicted poor OS in cholangiocarcinoma (HR=2.14, 95% CI: 1.30-3.50, P=0.003) and hepatocellular carcinoma (HR=1.91, 95% CI: 1.22-2.99, P =0.005) but not in gastric cancer (HR=0.97, 95% CI: 0.65-1.45, P=0.872), colorectal cancer (HR=1.18, 95% CI: 0.32-4.41, P=0.807), gallbladder cancer (HR=1.40, 95% CI: 0.84-2.34, P=0.198), and pancreatic cancer (HR=1.92, 95% CI: 0.99-3.72, P=0.053). Furthermore, high S100P expression was significantly associated with distant metastasis (OR=3.58, P=0.044), advanced clinical stage (OR=2.03, P=0.041) and recurrence (OR=1.66, P=0.007).

Conclusion: S100P might act as a prognostic indicator of non-gastrointestinal tract cancers.
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http://dx.doi.org/10.3389/fonc.2021.593728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973272PMC
March 2021

Short-wave infrared real-time high dynamic range imaging and display based on correlated double sampling.

Appl Opt 2021 Feb;60(6):1774-1779

A real-time high dynamic range (HDR) imaging and display method based on correlated double sampling is proposed for short-wave infrared (SWIR) cameras in order to effectively improve its range of brightness and contrast, as well as to obtain more image details. The method utilizes the correlated double sampling technique of the SWIR detector to extend the 14-bit raw image into a 16-bit HDR image and achieve 4 times the HDR imaging. Subsequently, a dynamic range compression process, including logarithmic mapping and histogram equalization, is performed for the 16-bit HDR image to be mapped to an 8-bit display. Finally, the experimental results show that the method can enrich the details of SWIR images under the premise of ensuring real-time imaging.
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http://dx.doi.org/10.1364/AO.415352DOI Listing
February 2021

Small Proline-Rich Protein 2B Facilitates Gastric Adenocarcinoma Proliferation via MDM2-p53/p21 Signaling Pathway.

Onco Targets Ther 2021 26;14:1453-1463. Epub 2021 Feb 26.

Department of Gastroenterology, Third Affiliated Hospital of Nanchang University, Nanchang, 330008, Jiangxi Province, People's Republic of China.

Background: The small proline-rich protein 2B (SPRR2B) was firstly reported as a member of the cross-linked envelope protein in keratinocytes. The effect of SPRR2B in gastric adenocarcinoma (GC) remains unclear. This study initially explored the clinical significance of SPRR2B in GC patients as well as its role in tumor progression.

Methods: Immunohistochemistry was performed to characterize the expression of SPRR2B in GC tissues and adjacent tissues. The relationship between SPRR2B expression and clinicopathological features of GC patients was analyzed by Chi-square test. Kaplan-Meier method and Cox regression analyses were utilized to identify the prognostic factors of GC. Overexpression and knockdown assays were conducted to investigate possible signaling pathways downstream of SPRR2B. Flow cytometry assays were performed to evaluate cell cycle and apoptosis. Xenograft experiments were performed to validate tumor-related role of SPRR2B in vivo.

Results: Both mRNA and protein levels of SPRR2B in cancerous tissue were significantly higher than those in non-cancerous tissues. Meanwhile, SPRR2B expression was significantly associated with tumor size and tumor stage. Survival analysis revealed SPRR2B as one of the independent prognosis factors for overall survival of GC patients. Cellular and xenografts data implicated that silencing SPRR2B blocked the cell cycle of GC cells perhaps through MDM2-p53/p21-CDK1 pathway, while overexpressing SPRR2B exhibited opposite effects.

Conclusion: Our data suggest that SPRR2B may serve as a novel prognostic marker in GC, which functions at least partially by MDM2-p53/p21-CDK1 signaling pathway.
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http://dx.doi.org/10.2147/OTT.S281032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924129PMC
February 2021

Observation of inflammation-induced mitophagy during stroke by a mitochondria-targeting two-photon ratiometric probe.

Analyst 2021 Apr;146(8):2632-2637

College of Bioresources and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China. and Shaanxi Collaborative Innovation Center of Industrial Auxiliary Chemistry & Technology, Shaanxi University of Science & Technology, Xi'an, 710021, China.

This study reports the development of a new, pH-sensitive, mitochondria-targeting two-photon ratiometric probe (Mito-BNO) for real-time tracking of mitophagy, a process that can be accelerated in brain tissue during stroke. Mito-BNO shows excellent capability for mitochondrial localisation (Pearson's correlation coefficient, r = 0.91), and can also effectively distinguish mitochondria from other subcellular organelles such as lysosomes and the endoplasmic reticulum (r = 0.40 and r = 0.33, respectively). Meanwhile, a rewarding pKa value (5.23 ± 0.03) and the pH reversibility suggest that Mito-BNO can track mitophagy in real time via confocal imaging. Most importantly, the relationship between mitophagy and neuroinflammation during stroke has been successfully demonstrated by evaluating the fluorescence of PC12 cells stained with Mito-BNO during an oxygen-glucose deprivation/reperfusion (OGD/R) process with and without anti-inflammatory treatment. The results indicate that the occurrence of mitophagy during stroke is caused by oxidative stress induced by neuroinflammation. This study will help further understanding stroke pathogenesis, can provide potential new targets for early diagnosis and treatment, and can also help to develop therapeutic drugs for stroke.
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http://dx.doi.org/10.1039/d1an00208bDOI Listing
April 2021

Rapid Identification of Inhibitors and Prediction of Ligand Selectivity for Multiple Proteins: Application to Protein Kinases.

J Phys Chem B 2021 03 2;125(9):2288-2298. Epub 2021 Mar 2.

Dalton Cardiovascular Research Center, Department of Physics and Astronomy, Department of Biochemistry, Institute for Data Science and Informatics, University of Missouri, Columbia, Missouri 65211, United States.

Rapid identification of inhibitors for a family of proteins and prediction of ligand specificity are highly desirable for structure-based drug design. However, sequentially docking ligands into each protein target with conventional single-target docking methods is too computationally expensive to achieve these two goals, especially when the number of the targets is large. In this work, we use an efficient ensemble docking algorithm for simultaneous docking of ligands against multiple protein targets. We use protein kinases, a family of proteins that are highly important for many cellular processes and for rational drug design, as an example to demonstrate the feasibility of investigating ligand selectivity with this algorithm. Specifically, 14 human protein kinases were selected. First, native docking calculations were performed to test the ability of our energy scoring function to reproduce the experimentally determined structures of the ligand-protein kinase complexes. Next, cross-docking calculations were conducted using our ensemble docking algorithm to study ligand selectivity, based on the assumption that the native target of an inhibitor should have a more negative (i.e., favorable) energy score than the non-native targets. Staurosporine and Gleevec were studied as examples of nonselective and selective binding, respectively. Virtual ligand screening was also performed against five protein kinases that have at least seven known inhibitors. Our quantitative analysis of the results showed that the ensemble algorithm can be effective on screening for inhibitors and investigating their selectivities for multiple target proteins.
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http://dx.doi.org/10.1021/acs.jpcb.1c00016DOI Listing
March 2021

Development of a comprehensive assessment model for coral reef island carrying capacity(CORE-CC).

Sci Rep 2021 Feb 16;11(1):3917. Epub 2021 Feb 16.

State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Coral reef islands provide precious living space and valuable ecological services for human beings, and its sustainability cannot be ignored under the pressure of human activities. Carrying capacity (CC) assessment has gradually become an important means to measure sustainability of islands. However, there is little comprehensive evaluation of the carrying capacity of coral reef islands, and traditional evaluation methods are difficult to express the social-ecological characteristics of coral reef islands. The present paper proposes a comprehensive assessment model for coral reef island carrying capacity (CORE-CC) which comprises dimensions of resources supply, environmental assimilative, ecosystem services, and socio-economic supporting. According to the characteristics of the coral reef islands, the core factors and indicators of each dimension are selected and the corresponding assessment index system of "pressure-support" is constructed. The assessment involves (1) identification of carrying dimensions and core factors, (2) pressure/support measurement and (3) assessment of carrying state. A case study is conducted in Zhaoshu Island of China, demonstrating the applicability of CORE-CC model and serving as a reference for adaptive management.
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http://dx.doi.org/10.1038/s41598-021-83481-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886852PMC
February 2021

Dopamine Receptor Antagonists, Radiation, and Cholesterol Biosynthesis in Mouse Models of Glioblastoma.

J Natl Cancer Inst 2021 Feb 9. Epub 2021 Feb 9.

Department of Radiation Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Background: Glioblastoma is the deadliest brain tumor in adults and the standard-of-care consists of surgery followed by radiation and treatment with temozolomide. Overall survival times for patients suffering from glioblastoma are unacceptably low indicating an unmet need for novel treatment options.

Methods: Using patient-derived HK-157, HK-308, HK-374, and HK-382 glioblastoma lines, the GL261 orthotopic mouse models of glioblastoma and HK-374 patient-derived orthotopic xenografts we tested the effect of radiation and the dopamine receptor antagonist quetiapine on glioblastoma self-renewal in vitro and survival in vivo. A possible resistance mechanism was investigated using RNA-Sequencing. The blood-brain-barrier-penetrating statin atorvastatin was used to overcome this resistance mechanism. All statistical tests were 2-sided.

Results: Treatment of glioma cells with the dopamine receptor antagonist quetiapine reduced glioma cell self-renewal in vitro and combined treatment of mice with quetiapine and radiation prolonged the survival of glioma-bearing mice. The combined treatment induced the expression of genes involved in cholesterol biosynthesis. This rendered GL261 and HK-374 orthotopic tumors vulnerable to simultaneous treatment with atorvastatin and further statistically significantly prolonged the survival of C57BL/6 (n = 10 to 16 mice per group; median survival not reached; Log-Rank test, p < 0.001) and NSG mice (n = 8 to 21 mice per group; hazard ratio = 3.96, 95% confidence interval = 0.29 to 12.40; Log-Rank test, p < 0.001), respectively.

Conclusions: Our results indicate promising therapeutic efficacy with the triple combination of quetiapine, atorvastatin and radiation treatment against glioblastoma without increasing the toxicity of radiation. With both drugs readily available for clinical use our study could be rapidly translated into a clinical trial.
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http://dx.doi.org/10.1093/jnci/djab018DOI Listing
February 2021

UV-Cured Transparent Silicone Materials with High Tensile Strength Prepared from Hyperbranched Silicon-Containing Polymers and Polyurethane-Acrylates.

ACS Omega 2021 Feb 22;6(4):2890-2898. Epub 2021 Jan 22.

Key Laboratory of Organosilicon Chemistry and Material Technology of Education Ministry, College of Material, Chemistry, and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.

Flexibility and mechanical performance are essential for transparent silicone materials applied in some optical and electronic devices; however, the tensile strength of transparent silicone materials is fairly low. To overcome this problem, a kind of UV-cured transparent flexible silicone material with quite a high tensile strength and elongation at break was developed through UV-initiated thiol-ene reaction by hyperbranched silicon-containing polymers (HBPs) with a thiol substitute and acrylate-terminated polyurethanes. Unexpectedly, it is found that both the tensile strength and elongation at break of the transparent silicone materials are extraordinarily high, which can reach 3.40 MPa and 270.0%, respectively. The UV-cured materials have good UV resistance ability because their transmittance is still as high as 93.4% (800 nm) even when aged for 40 min in a UV chamber of 10.6 mW cm. They exhibit outstanding adhesion to substrates, and the adhesion to a glass slide, wood, and a tin plate is grade 1. The promising results encourage us to further improve the mechanical performance of flexible transparent silicone materials by effective chemical modification strategies with HBPs. An attempt was made to apply the UV-cured materials in a Gel-Pak box and it could be proved that the UV-cured materials may be one of the good candidates for use as packaging or protecting materials of optical or electronics devices such as the Gel-Pak product.
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http://dx.doi.org/10.1021/acsomega.0c05243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860083PMC
February 2021

Correction: Age effect on gray matter volume changes after sleep restriction.

PLoS One 2021 4;16(2):e0246799. Epub 2021 Feb 4.

[This corrects the article DOI: 10.1371/journal.pone.0228473.].
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246799PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861364PMC
February 2021

Metformin reverses chemoresistance in non-small cell lung cancer via accelerating ubiquitination-mediated degradation of Nrf2.

Transl Lung Cancer Res 2020 Dec;9(6):2337-2355

Department of Thoracic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: The therapeutic efficacy of cisplatin-based chemotherapy for non-small cell lung cancer (NSCLC) is limited by drug resistance. In NSCLC, hyperactivation of nuclear factor erythroid 2-related factor 2 (Nrf2) counteracts oxidative stress to promote chemoresistance. Metformin-mediated downregulation of Nrf2 plays a pivotal role in overcoming drug resistance in NSCLC cells. Therefore, a deeper understanding of the molecular mechanisms of combination therapy and the role of Nrf2 in chemotherapeutic response is critical to clinical translation.

Methods: The effects of combination therapy with metformin and cisplatin on cell proliferation and apoptosis, intracellular reactive oxygen species (ROS) levels, and xenograft tumor formation were analyzed in NSCLC cells. Co-immunoprecipitation (co-IP) and Phos-tag assays were used to explore the mechanism of metformin-mediated Nrf2 suppression. Immunohistochemical (IHC) staining was performed to detect Nrf2 expression in matched tumor samples before and after neoadjuvant chemotherapy.

Results: Metformin was observed to synergistically augment cisplatin-induced cytotoxicity by strongly inhibiting the level of Nrf2, thereby weakening the antioxidant system and detoxification ability of Nrf2 and enhancing ROS-mediated apoptosis in NSCLC. The synergistic antitumor effect of combination therapy is blocked by treatment with the ROS scavenger N-acetyl cysteine (NAC) as well as overexpression of Nrf2 and its downstream antioxidant protein. Mechanistically, metformin extensively dephosphorylates Nrf2 by attenuating the interaction between Nrf2 and extracellular signal-regulated kinases 1/2 (ERK1/2), which then restores its polyubiquitination and accelerates its proteasomal degradation. Moreover, for the first time, an association of non-decreased Nrf2 expression in patients after neoadjuvant chemotherapy with poor survival and chemoresistance in NSCLC was revealed.

Conclusions: Our findings illustrate the mechanism of metformin-mediated Nrf2 degradation through posttranslational modifications (PTMs), which weakens the ROS defense system in NSCLC. Fluctuations in Nrf2 expression have a strong predictive ability for chemotherapeutic response in neoadjuvant NSCLC patients. Targeting of the Nrf2 pathway could be a therapeutic strategy for overcoming chemoresistance, with metformin as the first choice for this strategy.
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http://dx.doi.org/10.21037/tlcr-20-1072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815349PMC
December 2020

Epitaxial Growth of Two-Dimensional Insulator Monolayer Honeycomb BeO.

ACS Nano 2021 Feb 22;15(2):2497-2505. Epub 2021 Jan 22.

Department of Physics, The University of Texas at Austin, Austin, Texas 78712, United States.

The emergence of two-dimensional (2D) materials launched a fascinating frontier of flatland electronics. Most crystalline atomic layer materials are based on layered van der Waals materials with weak interlayer bonding, which naturally leads to thermodynamically stable monolayers. We report the synthesis of a 2D insulator composed of a single atomic sheet of honeycomb structure BeO (h-BeO), although its bulk counterpart has a wurtzite structure. The h-BeO is grown by molecular beam epitaxy (MBE) on Ag(111) thin films that are also epitaxially grown on Si(111) wafers. Using scanning tunneling microscopy and spectroscopy (STM/S), the honeycomb BeO lattice constant is determined to be 2.65 Å with an insulating band gap of 6 eV. Our low-energy electron diffraction measurements indicate that the h-BeO forms a continuous layer with good crystallinity at the millimeter scale. Moiré pattern analysis shows the BeO honeycomb structure maintains long-range phase coherence in atomic registry even across Ag steps. We find that the interaction between the h-BeO layer and the Ag(111) substrate is weak by using STS and complementary density functional theory calculations. We not only demonstrate the feasibility of growing h-BeO monolayers by MBE, but also illustrate that the large-scale growth, weak substrate interactions, and long-range crystallinity make h-BeO an attractive candidate for future technological applications. More significantly, the ability to create a stable single-crystalline atomic sheet without a bulk layered counterpart is an intriguing approach to tailoring 2D electronic materials.
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http://dx.doi.org/10.1021/acsnano.0c06596DOI Listing
February 2021

GPR120 Agonist GW9508 Ameliorated Cellular Senescence Induced by ox-LDL.

ACS Omega 2020 Dec 8;5(50):32195-32202. Epub 2020 Dec 8.

Department of Cardiology, Dongguan Songshanhu Central Hospital, Dongguan, Guangdong 523326, China.

Oxidized low-density lipoprotein (ox-LDL)-induced endothelial senescence is involved in the pathogenesis of atherosclerosis and many cardiovascular diseases. G-protein-coupled receptor 120 (GPR120), a type of orphan G-protein-coupled receptors (GPRs), plays a vital role in mediating anti-inflammatory and insulin-sensitizing effects. The biological function of GPR120 in vascular endothelial cells is largely unknown. The human aortic endothelial cells (HAECs) were treated with ox-LDL (100 μg/mL) in the presence or absence of GW9508 (50 μM) or AH9614 (1 μM) for 24 h. The LDH assay was used to determine cell death. The dihydroethidium (DHE) staining assay was used to measure intracellular levels of reactive oxidative species (ROS), and a senescence β-galactosidase assay kit was used to determine endothelial senescence. Gene and protein expressions were measured using real-time polymerase chain reaction (PCR) and western blot analysis, respectively. Ox-LDL treatment decreased the expression of GPR120 by more than half in HAECs. Typically, 100 μg/mL of ox-LDL- induced 35.2% LDH release, which was reduced to 16.9% by 50 μM GW9508, the agonist of GPR120. Importantly, GW9508 relieved cytotoxicity and suppressed the ox-LDL-induced increase in the activity of senescence-associated β-galactosidase (SA-β-Gal) (from 3.3-fold to 1.6-fold of the control group) and the generation of cellular reactive oxidative species (ROS) (from 3.8-fold to 1.6-fold of the control group). Furthermore, we found that GW9508 ameliorated ox-LDL-induced endothelial cell cycle arrest at the G0/G1 phase and the expression of key senescence proteins, including p53 and plasminogen activator inhibitor-1(PAI-1). Mechanistically, we showed that GW9508 promoted ox-LDL-induced transcriptional factor NF-E2-related factor 2 (NRF2) (increase by 47.3%) translocation into the nucleus. The effect of GW9508 is dependent on its receptor GPR120, the blockage of which by its specific antagonist, AH7614, abolished the antisenescence effect of GW9508. Collectively, this study revealed the protective effect of GPR120 activation in vascular endothelial cells, implying that GPR120 is a promising therapeutic target for the treatment of cardiovascular diseases.
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http://dx.doi.org/10.1021/acsomega.0c03581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758881PMC
December 2020

High Efficiency and Low Migration Hyperbranched Silicone Contain Macrophotoinitiators for UV-Cured Transparent Coatings.

Polymers (Basel) 2020 Dec 16;12(12). Epub 2020 Dec 16.

Key Laboratory of Organosilicon Chemistry and Material Technology of Education Ministry, College of Material, Chemistry, and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.

A kind of hyperbranched silicone containing macrophotoinitiators (HBSMIs) were synthesized from 2-hydroxy-2-methyl-1-phenyl propanone (HMPP) and the UV-curing behaviors of HBSMIs were investigated in UV-cured transparent polyurethane-acrylate (PUA) coatings. HBSMIs show higher UV-initiating efficiency than HMPP. The migration of HBSMIs from the UV-cured coatings can be as low as 1.7-6.0 wt%, which is obviously lower than the migration of HMPP. There is a remarkable improvement of the tensile strength of the UV-cured materials initiated by HBSMI in comparison to that of the materials prepared with the same PUA initiated by HMPP. Especially for the UV-cured materials prepared from PUA with 20 wt% 1,1,1-tris(hydroxymethyl)propane (TMP), the tensile strength and the strain at break increased from 6.81 MPa to 12.14 MPa and from 43.0% to 71.9%, respectively. The fraction of improvement for the tensile strength and the strain at break is as high as 78.9% and 67.2%, respectively. The coatings prepared with HBSMI also have better UV resistance ability than those coatings prepared with HMPP because they turn slightly yellow when they are aged by UV for about 15 min while the coating prepared with 4 wt% of HMPP will turn yellow only aged by UV for 2 min. These results suggest that preparation hyperbranched silicone containing macrophotoinitiators will be one of the good strategies to improve the curing efficiency of the UV-curing system, reduce the migration of UV initiator from cured material, improve the mechanical and UV resistance performance of UV-cured materials.
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http://dx.doi.org/10.3390/polym12123005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766499PMC
December 2020

[Physiochemical properties and microbial community characteristics of rhizosphere soil in plantation].

Ying Yong Sheng Tai Xue Bao 2020 Oct;31(10):3365-3375

Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China.

We explored the impacts of different mixed trees on the improvement of soil microecological environment in rhizosphere of including pure plantation (WC), mixed and plantation (WJ), mixed and plantation (WA). Soil physical and chemical properties were analyzed. The characteristics and distribution of soil microbes in the rhizosphere were measured by the methods of Biolog-Eco micro plate and phospholipid fatty acid methyl ester (PLFA). Soil water content, soil pH, organic matter, total nitrogen, total potassium content and the activities of sucrase, urease and acid phosphatase in rhizosphere soil of WA were significantly higher than those of WC and WJ, without difference between WC and WJ. There were no significant differences in the contents of nitrate nitrogen, ammonium nitrogen, and available potassium between WA and WJ, which were obviously higher than those in WC. There were significant differences in total phosphorus and available phosphorus contents among the three stands, with an order of WJ>WA>WC. The average color change rate (AWCD), Shannon index, Simpson index, McIntosh index and the utilization of six types of carbon source substrates in microorganisms were the highest in the rhizosphere soil of WA, followed by WJ and WC. Results of principal component analysis showed that carbohydrates, amino acids and phenolic acids were the main carbon sources for microbial utilization. In WA, the PLFA content of rhizosphere soil microorganism, bacteria, fungi and actinomycetes was the highest, followed by WJ and WC. There were significant positive correlation between soil physical and che-mical properties and the microbial characteristics. Combining the physical and chemical properties of soil and the functional and structural characteristics of microbial communities, the mixed and plantation may be most conductive to the improvement of the rhizosphere microecological environment and increase soil available nutrients at the young tree phase of .
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http://dx.doi.org/10.13287/j.1001-9332.202010.019DOI Listing
October 2020

The Reference Intervals and Roles of GIR, HOMA and QUICKI Indexes to Judge Insulin Resistance/Insufficiency for Newly Diagnosed Diabetes Mellitus.

Clin Lab 2020 Nov;66(11)

Background: To establish the reference intervals of GIR, HOMA, and QUICKI index and to identify the clinical value of the three indexes for newly diagnosed diabetes mellitus.

Methods: The results of fasting glucose and insulin were acquired for 123 healthy individuals using Roche cobas-8000 to establish reference intervals of GIR, HOMA, and QUICKI based on Clinical and Laboratory Standards Institute (CLSI) EP28-A3. Meanwhile, 36 newly diagnosed type 1 and type 2 diabetes mellitus (DM) patients were enrolled to judge the effect of insulin resistance/insufficiency using the three indexes based on clinical initial treat-ment procedures. All the data were acquired from Wangjing Hospital, China Academy of Traditional Chinese Medicine.

Results: The reference intervals of GIR, HOMA, and QUICKI were 5.83 - 21.15, 0.87 - 4.22, and 0.309 - 0.392, respectively. Concerning to GIR, HOMA, and QUICKI, there were 57.7% (15/26), 80.8% (21/26), and 80.8% (21/26) outside of the reference limit among type 2 DM patients, respectively; The area under the curve (AUC) of the GIR > 10.937, HOMA < 5.436, and QUICKI > 0.299 were 0.937 (95% CI 0.681 - 1.000), 0.689 (95% CI 0.510 - 0.868), and 0.689 (95% CI 0.510 - 0.868) by ROC curves when insulin insufficiency was judged based on whether insulin was included in initial treatment procedures. There concordance rates were 77.8% (28/36), 50% (18/36), and 50% (18/36) using the three indexes, GIR, HOMA, and QUICKI, respectively.

Conclusions: We established reference intervals for GIR, HOMA, and QUICKI. HOMA and QUICKI were more reliable indexes to identify insulin resistance among type 2 DM patients, but GIR was a more reliable index to identify insulin relatively or absolutely insufficiency than HOMA and QUICKI among DM patients.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200113DOI Listing
November 2020

Indole-based alkaloids from Ophiocordyceps xuefengensis.

Phytochemistry 2021 Jan 5;181:112536. Epub 2020 Nov 5.

Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 410013, China. Electronic address:

Seven undescribed indole-based alkaloids, xuefengins A-D and xuefenglasins A-C, were isolated from natural Ophiocordyceps xuefengensis, along with six known alkaloids. Their structures were elucidated by comprehensive spectroscopy, with absolute configurations confirmed by comparison with calculated electronic circular dichroism spectra. Eleven of the isolates were tested for cytotoxicity against the U937, NB4, MCF-7, Hep G2, and A549 cancer cell lines. Two compounds exhibited moderate activities, with IC values of 2.83-25.68 μM and 1.54-12.16 μM. Further pharmacological studies showed that these two compounds inhibit cell proliferation by inducing apoptosis, and decreasing p38 and caspase-3 levels in A549 cells.
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http://dx.doi.org/10.1016/j.phytochem.2020.112536DOI Listing
January 2021

Retrospective analysis of LNM risk factors and the effect of chemotherapy in early colorectal cancer: A Chinese multicenter study.

BMC Cancer 2020 Nov 5;20(1):1067. Epub 2020 Nov 5.

Department of Gastroenterology, the First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, 330006, Jiangxi, China.

Background: Estimating the risk of lymph node metastasis (LNM) is crucial for determining subsequent treatments following curative resection of early colorectal cancer (ECC). This multicenter study analyzed the risk factors of LNM and the effectiveness of postoperative chemotherapy in patients with ECC.

Methods: We retrospectively analyzed the data of 473 patients with ECC who underwent general surgery in five hospitals between January 2007 and October 2018. The correlations between LNM and sex, age, tumor size, tumor location, endoscopic morphology, pathology, depth of invasion and tumor budding (TB) were directly estimated based on postoperative pathological analysis. We also observed the overall survival (OS) and recurrence in ECC patients with and without LNM after matching according to baseline measures.

Results: In total, 473 ECC patients were observed, 288 patients were enrolled, and 17 patients had LNM (5.90%). The univariate analysis revealed that tumor size, pathology, and lymphovascular invasion were associated with LNM in ECC (P = 0.026, 0.000, and 0.000, respectively), and the multivariate logistic regression confirmed that tumor size, pathology, and lymphovascular invasion were risk factors for LNM (P = 0.021, 0.023, and 0.001, respectively). There were no significant differences in OS and recurrence between the ECC patients with and without LNM after matching based on baseline measures (P = 0.158 and 0.346, respectively), and no significant difference was observed between chemotherapy and no chemotherapy in ECC patients without LNM after surgery (P = 0.729 and 0.052).

Conclusion: Tumor size, pathology, and lymphovascular invasion are risk factors for predicting LNM in ECC patients. Adjuvant chemotherapy could improve OS and recurrence in patients with LNM but not always in ECC patients without LNM.
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http://dx.doi.org/10.1186/s12885-020-07363-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643346PMC
November 2020

Repeated radon exposure induced lung injury and epithelial-mesenchymal transition through the PI3K/AKT/mTOR pathway in human bronchial epithelial cells and mice.

Toxicol Lett 2020 Nov 16;334:4-13. Epub 2020 Sep 16.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215123, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, 215123, China. Electronic address:

Radon exposure is the most frequent cause of lung cancer in non-smokers. The high linear energy transfer alpha-particles from radon decay cause the accumulation of multiple genetic changes and lead to cancer development. Epithelial-mesenchymal transition (EMT) plays an important role in oncogenesis. However, the mechanisms underlying chronic radon exposure-induced EMT attributed to carcinogenesis are not understood. This study aimed to explore the EMT and potential molecular mechanisms induced by repeated radon exposure. The EMT model of 16HBE and BEAS-2B cells was established with radon exposure (20000 Bq/m, 20 min each time every 3 days). We found repeated radon exposure facilitated epithelial cell migration, proliferation, reduced cell adhesion and ability to undergo EMT through a decrease in epithelial markers and an increase in mesenchymal markers. Radon regulated the expression of matrix metalloproteinase 2 (MMP2) and tissue inhibitors of metalloproteinase 2 (TIMP2) to disrupt the balance of MMP2/TIMP2. In vivo, BALB/c mice were exposed to 10 Bq/m radon gas for cumulative doses of 60 and 120 Working Level Months (WLM). Radon inhalation caused lung damage and fibrosis in mice, which was aggravated with the increase of exposure dose. EMT-like transformation also occurred in lung tissues of radon-exposure mice. Moreover, radon radiation increased p-PI3K, p-AKT and p-mTOR in cells and mice. Radon reduced the GSK-3β level and elevated the active β-catenin in 16HBE cells. The m-TOR and AKT inhibitors attenuated radon exposure-induced EMT by regulation related biomarkers. These data demonstrated that radon exposure induced EMT through the PI3K/AKT/mTOR pathway in epithelial cells and lung tissue.
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http://dx.doi.org/10.1016/j.toxlet.2020.09.008DOI Listing
November 2020

Simultaneous Determination of Metal Ions in Zinc Sulfate Solution Using UV-Vis Spectrometry and SPSE-XGBoost Method.

Sensors (Basel) 2020 Aug 31;20(17). Epub 2020 Aug 31.

School of Automation, Central South University, Changsha 410083, China.

Excessive discharge of heavy metal ions will aggravate environment pollution and threaten human health. Thus, it is of significance to real-time detect metal ions and control discharge in the metallurgical wastewater. We developed an accurate and rapid approach based on the singular perturbation spectrum estimator and extreme gradient boosting (SPSE-XGBoost) algorithms to simultaneously determine multi-metal ion concentrations by UV-vis spectrometry. In the approach, the spectral data is expanded by multi-order derivative preprocessing, and then, the sensitive feature bands in each spectrum are extracted by feature importance (VI score) ranking. Subsequently, the SPSE-XGBoost model are trained to combine multi-derivative features and to predict ion concentrations. The experimental results indicate that the developed "Expand-Extract-Combine" strategy can not only overcome problems of background noise and spectral overlapping but also mine the deeper spectrum information by integrating important features. Moreover, the SPSE-XGBoost strategy utilizes the selected feature subset instead of the full-spectrum for calculation, which effectively improves the computing speed. The comparisons of different data processing methods are conducted. It outcomes that the proposed strategy outperforms other routine methods and can profoundly determine the concentrations of zinc, copper, cobalt, and nickel with the lowest RMSEP. Therefore, our developed approach can be implemented as a promising mean for real-time and on-line determination of multi-metal ion concentrations in zinc hydrometallurgy.
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http://dx.doi.org/10.3390/s20174936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506957PMC
August 2020

Carnosic acid protects against pressure overload-induced cardiac remodelling by inhibiting the AKT/GSK3β/NOX4 signalling pathway.

Exp Ther Med 2020 Oct 7;20(4):3709-3719. Epub 2020 Aug 7.

Department of Cardiology, Taihe Hospital of Shiyan, Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.

Oxidative stress and apoptosis serve an important role in the development of pressure overload-induced cardiac remodelling. Carnosic acid (CA) has been found to exert antioxidant and anti-apoptotic effects. The present study investigated the underlying mechanism of CA protection and whether this effect was exerted against pressure overload-induced cardiac remodelling. Aortic banding (AB) surgery was performed to induce cardiac remodelling. Mice were randomly divided into four groups (n=15/group): i) Sham + vehicle; ii) sham + CA; iii) AB + vehicle; and iv) AB + CA. After 2 days of AB, 50 mg kg CA was administered orally for 12 days. Echocardiography, histological analysis and molecular biochemistry techniques were performed to evaluate the roles of CA. CA treatment decreased cardiac hypertrophy, fibrosis, oxidative stress and apoptosis in mice challenged with pressure overload. CA also decreased the cross-sectional area of cardiomyocytes and the mRNA and protein expression levels of hypertrophic markers. Furthermore, CA treatment decreased collagen deposition, α-smooth muscle actin expression and the mRNA and protein expression of various fibrotic markers. Additionally, CA reversed the AB-mediated increase in NAPDH oxidase (NOX) 2, NOX4 and 4-hydroxynonenal levels. The number of apoptotic cells was decreased following CA treatment following under conditions of pressure overload. CA also suppressed the activation of AKT and glycogen synthase kinase 3 β (GSK3β) in mice challenged with AB. The present results suggested that CA could inhibit pressure overload-induced cardiac hypertrophy and fibrosis by suppressing the AKT/GSK3β/NOX4 signalling pathway. Therefore, CA may be a promising therapy for cardiac remodelling.
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http://dx.doi.org/10.3892/etm.2020.9109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444384PMC
October 2020

Synergistic effects of sodium adipate/triethylene glycol on the plasticization and retrogradation of corn starch.

Carbohydr Res 2020 Oct 7;496:108112. Epub 2020 Aug 7.

College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, China.

Starch retrogradation resulted in bad performance and taste of starch products. In this study, the corn starch films modified by sodium adipate and triethylene glycol were prepared by solution casting. The retrogradation of modified starch films were studied by FT-IR, XRD, SEM, tension test and UV-Vis. The results showed that sodium adipate was more effective than triethylene glycol in inhibiting the short-term retrogradation of starch, and triethylene glycol was more effective than sodium adipate in inhibiting the long-term retrogradation of starch. However, the mixture of sodium adipate and triethylene glycol, especially 15% adipic acid and 10% triethylene glycol, showed synergistic effects on the short-term and long-term retrogradation of starch. The starch film with 15% adipic acid and 10% triethylene glycol had the highest elongation at break, the best transmittance, the lowest change rate of elongation at break, and the lowest moisture content among all the recipes.
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http://dx.doi.org/10.1016/j.carres.2020.108112DOI Listing
October 2020

Object detection based on an adaptive attention mechanism.

Sci Rep 2020 07 9;10(1):11307. Epub 2020 Jul 9.

School of Computer Science and Technology, Xidian University, Xi'an, 710071, China.

Object detection is an important component of computer vision. Most of the recent successful object detection methods are based on convolutional neural networks (CNNs). To improve the performance of these networks, researchers have designed many different architectures. They found that the CNN performance benefits from carefully increasing the depth and width of their structures with respect to the spatial dimension. Some researchers have exploited the cardinality dimension. Others have found that skip and dense connections were also of benefit to performance. Recently, attention mechanisms on the channel dimension have gained popularity with researchers. Global average pooling is used in SENet to generate the input feature vector of the channel-wise attention unit. In this work, we argue that channel-wise attention can benefit from both global average pooling and global max pooling. We designed three novel attention units, namely, an adaptive channel-wise attention unit, an adaptive spatial-wise attention unit and an adaptive domain attention unit, to improve the performance of a CNN. Instead of concatenating the output of the two attention vectors generated by the two channel-wise attention sub-units, we weight the two attention vectors based on the output data of the two channel-wise attention sub-units. We integrated the proposed mechanism with the YOLOv3 and MobileNetv2 framework and tested the proposed network on the KITTI and Pascal VOC datasets. The experimental results show that YOLOv3 with the proposed attention mechanism outperforms the original YOLOv3 by mAP values of 2.9 and 1.2% on the KITTI and Pascal VOC datasets, respectively. MobileNetv2 with the proposed attention mechanism outperforms the original MobileNetv2 by a mAP value of 1.7% on the Pascal VOC dataset.
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http://dx.doi.org/10.1038/s41598-020-67529-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347846PMC
July 2020

Fabrication of Transparent UV-Cured Coatings with Allyl-Terminated Hyperbranched Polycarbosilanes and Thiol Silicone Resins.

ACS Omega 2020 Jun 17;5(25):15311-15316. Epub 2020 Jun 17.

Key Laboratory of Organosilicon Chemistry and Material Technology of Education Ministry, Hangzhou Normal University, Hangzhou 311121, China.

To improve thermal stability and hardness of UV-cured materials, a series of UV-cured solvent-free coatings were prepared from allyl-terminated hyperbranched polycarbosilanes and thiol silicone resins. The silicone coatings prepared have pencil hardness of 4-9 H, water absorption no more than 0.04 wt %, and transmittance higher than 94%. The temperature for the coatings' starting thermal decomposition is higher than 236 °C; especially, that of the coating prepared with G1 is as high as 371.1 °C. The UV-cured coatings in this work exhibit much higher pencil hardness than and superior thermal stability to those reported previously.
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http://dx.doi.org/10.1021/acsomega.0c01338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331026PMC
June 2020

Stem cells: a potential treatment option for kidney diseases.

Stem Cell Res Ther 2020 06 25;11(1):249. Epub 2020 Jun 25.

Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.

The prevalence of kidney diseases is emerging as a public health problem. Stem cells (SCs), currently considered as a promising tool for therapeutic application, have aroused considerable interest and expectations. With self-renewal capabilities and great potential for proliferation and differentiation, stem cell therapy opens new avenues for the development of renal function and structural repair in kidney diseases. Mounting evidence suggests that stem cells exert a therapeutic effect mainly by replacing damaged tissues and paracrine pathways. The benefits of various types of SCs in acute kidney disease and chronic kidney disease have been demonstrated in preclinical studies, and preliminary results of clinical trials present its safety and tolerability. This review will focus on the stem cell-based therapy approaches for the treatment of kidney diseases, including various cell sources used, possible mechanisms involved, and outcomes that are generated so far, along with prospects and challenges in clinical application.
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http://dx.doi.org/10.1186/s13287-020-01751-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318741PMC
June 2020