Publications by authors named "Fatma Yılmaz"

137 Publications

Evaluation of the diagnostic performance of an oral fluid screening test device for substance abuse at traffic controls.

Clin Biochem 2021 Apr 18. Epub 2021 Apr 18.

Department of Medical Biochemistry, Ankara Yıldırım Beyazıt University Faculty of Medicine, Ankara, Turkey.

Introduction: The aim of this study was to evaluate the analytical performance of the Kite Biotechnology Oral fluid (OF) screening test device, which is used for roadside screening of cannabis, opiates, amphetamines, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), cocaine and benzodiazepines by comparing samples with matched plasma samples, analysed via liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation.

Methods: OF and plasma samples were obtained simultaneously from a total of 100 subjects. OF samples were analysed by OF screening test based on immunochromatography. The OF screening test cut-off values were 50 ng/mL for amphetamines (d-amphetamine) and methamphetamine/MDMA (d-methamphetamine), 30 ng/mL for cocaine (benzoylecgonine), 40 ng/mL for opiates (morphine), 20 ng/mL for benzodiazepines (nordazepam), and 25 ng/mL for cannabis (Δ-tetrahydrocannabinol). LC-MS/MS method validation was performed according to the CLSI C62-A recommendations with the following parameters: matrix effect, lower limit of quantification (LLOQ), linearity, intra-day and inter-day precision and accuracy.

Results: The overall specificity, accuracy and negative predictive values (NPV) were acceptable and met the DRUID standard of >80%. The OF screening test device showed good sensitivity for cocaine, amphetamines and opiates, whereas it indicated poor sensitivity for methamphetamine/MDMA (66.7%) and failed to detect cannabis and benzodiazepines.

Conclusion: The present study is the first report to evaluate the Kite Biotechnology OF screening test device. The diagnostic performance of the OF screening test device was acceptable for opiates, cocaine and amphetamines, but it was insufficient for methamphetamine/MDMA, benzodiazepines and cannabis because of sensitivity issues.
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http://dx.doi.org/10.1016/j.clinbiochem.2021.04.009DOI Listing
April 2021

Hypercalcemia associated with the interaction between all trans retinoic acid and posaconazole in an acute promyelocytic leukemia case.

J Oncol Pharm Pract 2021 Apr 13:10781552211007889. Epub 2021 Apr 13.

Department of Hematology, Diskapi Yildirim Beyazit Training and Research Hospital, University of Health Sciences, Ankara, Turkey.

Introduction: All-trans retinoic acid (ATRA) is a physiological metabolite of vitamin A and it is used for the treatment of acute promyelocytic leukemia (APL). Hypercalcemia is a rare side effect of ATRA and it may be potentiated after interaction of ATRA with azole group antifungals. Herein, we have reported an APL case with hypercalcemia that is caused by the interaction of ATRA and posaconazole.

Case Report: A 49-year-old female patient was diagnosed as APL after the examinations performed upon the detection of pancytopenia when she had presented with the complaints of widespread bruising and fever. After the initiation of posaconazole and ATRA, her serum calcium levels begin to increase (10.3 to 11.1mg/dl). Her vitamin D level was 21.9 ng/ml and PTH 17.8 pg/ml, both were in the normal ranges. The Drug Interaction Probability Scale score of our case was calculated as 6, indicating that the probable adverse drug reaction. Therefore, the high level of serum calcium was attributed to the interaction between ATRA and posaconazole.

Management & Outcome: Although hypercalcemia with ATRA and other antifungal agents have been previously reported in the literature, this is the first report of hypercalcemia with the concomitant use of ATRA and posaconazole.

Discussion: This case highlights the importance of monitoring ATRA's side effects when it is used in combination with drugs inhibiting the cytochrome P450 enzymes. In conclusion, the concomitant use of posaconazole and ATRA may lead to hypercalcemia and serum calcium levels return to normal ranges with the discontinuation of these drugs.
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http://dx.doi.org/10.1177/10781552211007889DOI Listing
April 2021

Effect of acupressure on procedural pain before heel lancing in neonates.

J Tradit Chin Med 2021 04;41(2):331-337

Research Assistant, RN, MSN, Faculty of Health Sciences, Pediatric Nursing Department, Canakkale Onsekiz Mart University, Canakkale 17020, Turkey.

Objective: To investigate the effect of acupressure applied to UB60 and K3 acupuncture points in order to relieve the procedural pain caused by heel lancing blood sampling process in the term newborns.

Methods: The data were collected by using the Information Form and the Neonatal Infant Pain Scale. Acupressure applied for 3 min before heel lancing blood sampling in the newborns in the experimental group (n = 31). No intervention was applied to newborns in the control group (n = 32).

Results: A significant difference was found between mean scores of the newborns in the control and acupressure group in favor of the acupressure group in terms of heart rate during and after the procedure, oxygen saturation before, during and after the procedure, duration of crying during and after the procedure (P < 0.05). It was found that there was a significant difference between groups in terms of Neonatal Infant Pain Scale mean scores during (P = 0.001) and after the procedure (P < 0.05), and the difference was found to be in favor of the acupressure group.

Conclusion: As a result, acupressure was found to be an effective method in relieving pain caused by heel lancing blood sampling in newborns.
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April 2021

Assessment of Galectin-1, Galectin-3, and PGE2 Levels in Patients with COVID-19.

Jpn J Infect Dis 2021 Mar 31. Epub 2021 Mar 31.

Medical Biochemistry Laboratory, Ministry of Health Ankara City Hospital, Turkey.

It is important to determine the inflammatory biomarkers in the severity of Coronavirus Disease 2019 (COVID-19) with the emergence of the pandemic. Galectins and prostaglandins play important roles in the regulation of immune and inflammatory responses. Therefore, this study aimed to investigate Galectin-1 (Gal-1), Galectin-3 (Gal-3), and prostaglandin E2 (PGE2) levels in patients with COVID-19. Gal-1, Gal-3, and PGE2 serum concentrations were measured using enzyme-linked immunosorbent analysis (ELISA) on 84 COVID-19 patients (severe=29 and nonsevere=55) and 56 healthy controls. In this study, the increased levels of Gal-1 (median, 9.86, 6.35, 3.67 ng/ml), Gal-3 (median, 415.31, 326.33, 243.13 pg/ml)and PGE2 (median, 193.17, 192.58, 124.62 pg/ml) levels were found in patients with COVID-19 than healthy controls (p<0.001 for all). In the severe group, Gal-3 levels were higher while there were no differences in Gal-1 and PGE2 levels (p=0.011, p=0.263, p=0.921, respectively). There was a positive correlation between serum Gal-1 and Gal-3 levels (ρ=0.871, p<0.001). Gal-3, C-reactive protein, lymphocyte count, and age were found as independent predictors of the disease severity (p=0.002, p=0.001, p=0.007, and p=0.003, respectively). With the emergence of effective drug needs in the COVID-19 pandemic, differentiation of severe disease is important. Gal-3 could be a potential prognostic biomarker of COVID-19.
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http://dx.doi.org/10.7883/yoken.JJID.2021.020DOI Listing
March 2021

The Diagnostic Value of Superb Microvascular Imaging in Prediction of Uterine Artery Embolization Treatment Response in Uterine Leiomyomas.

J Ultrasound Med 2021 Feb 18. Epub 2021 Feb 18.

Cerrahpasa Medical Faculty, Radiology Department, Istanbul University, Istanbul, Turkey.

Objectives: We aimed to determine if superb microvascular imaging (SMI) can predict response to uterine artery embolization (UAE) as compared with power Doppler ultrasound.

Methods: The blood flow and the volume of the dominant leiomyoma was evaluated by power Doppler ultrasonography (PDUS) and SMI 1 day before and 3 months after the UAE procedure. SMI and PDUS blood flow were classified to 4 grades of vascularity. The change in fibroid volume in Grades 0-2 (hypovascular group) was compared to the hypervascular Grade 3 group.

Results: Twenty-eight women (mean age, 40.9 years; range, 33-53 years) were examined with PDUS and SMI before and 3 months after UAE. The volume reduction was statistically significantly higher hypervascular group (P < .05). When we accept 30% or more volume reduction as a good response to UAE, the positive predictive value, negative predictive value, sensitivity, specificity, and accuracy of SMI were 100, 64, 73.6, 100, and 82.1%, respectively. There was excellent agreement between the two blinded observers in SMI measurements.

Conclusions: SMI, with its high reproducibility, provides further microvessel information than PDUS in uterine fibroids. It may be a useful tool in prediction of response to UAE treatment and improve counseling and patient selection for UAE versus medical or surgical treatment options.
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http://dx.doi.org/10.1002/jum.15647DOI Listing
February 2021

Readability, understandability, and quality of retinopathy of prematurity information on the web.

Birth Defects Res 2021 Feb 17. Epub 2021 Feb 17.

Department of Child Health and Disease, Dr. Ali Kemal Belviranli Hospital for Obstetrics and Pediatrics, Konya, Turkey.

Background: The purpose of this study is to assess the readability, understandability, and quality of information on retinopathy of prematurity presented at websites frequently visited by parents.

Methods: A total of 220 websites were assessed, which were recruited by searching for "retinopathy of prematurity" at the Google search engine. The readability of each web page was assessed by Flesch Reading Ease Score, Gobbledygook's Gunning Frequency, Flesch Kincaid Grade Level, Coleman Liau score, The Simple Measure of Gobbledygook, Fry Graph Readability Formula, and Automated readability score. The understandability of the web pages included in the study was measured by using the Patient Education Materials Assessment Tool. Quality was evaluated using Health in Net code and JAMA. The ALEXA traffic tool was used to reference the domains' popularity and visibility.

Results: Sixty-four websites were included to the study. The average Flesch Reading Ease Score was 50.1 ± 11.4, Gunning Frequency of Gobbledygook level was 13.4 ± 2.5, The Flesch-Kincaid Grade level was 10.7 ± 2.2, Coleman Liau level was 10.8 ± 1.7, Simple Measure of Gobbledygook level was 10.0 ± 1.9, and Fry Graph Readability Formula 11.9 ± 2.7, Automated readability score 10.4 ± 2.5. The average understandability score for all website-based patient education materials was 76.9 ± 15.2. Total JAMA Benchmark score is 2.27 ± 1.14 (range from 1 to 4). The quality of information at most websites were determined by our chosen assessments to not to be good.

Conclusion: Websites addressed to parents for retinopathy of prematurity had found to have high understandability. It was concluded based on this study that readability and quality of presented written materials at online sources need to be improved.
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http://dx.doi.org/10.1002/bdr2.1883DOI Listing
February 2021

Is there a relationship between causative microorganisms and hearing loss in neonatal sepsis?

Birth Defects Res 2021 Jan 11. Epub 2021 Jan 11.

Department of Pediatri, Dr Ali Kemal Belviranli Hospital for Obstetrics and Pediatrics, Konya, Turkey.

Background: The aim of the present study was to determine the possible relationship between cultured microorganisms and hearing loss in infants admitted to the neonatal intensive care unit (NICU) who could not pass a standard hearing test.

Method: The medical records of infants treated at the NICU were retrospectively evaluated. The patients were first divided into two groups, and group 1 was divided into two subgroups: Group 1 included patients with hearing loss accompanied by proven sepsis caused by either gram-negative (group 1A) or gram-positive (group 1B) bacteria, and group 2 included patients with clinical sepsis. The groups were compared with potential risk factors related to hearing loss.

Results: Between January 2014 and January 2019, the cases of 3,800 infants admitted to the NICU were reviewed. Of 3,548 living babies, the Auditory Brainstem Response (ABR) test showed that 35 infants (0.98%) were diagnosed with hearing loss. In 12 infants with hearing loss, microbial growth in the blood cultures was detected, whereas in the remaining 23, the blood cultures were negative. Of the cases with microbial growth, five were gram negative and seven were gram positive. In the comparison of groups 1A, 1B, and 2, there were statistically significant differences in terms of risk factors such as low birth weight (p = .048), neonatal hospitalization time (p = .001), free oxygen support (p = .001), intraventricular bleeding (p = .001), loop diuretic use (p = .001), and blood transfusion (p = .048).

Conclusion: The relationship between hearing loss and microorganisms causing sepsis could not be determined in this research.
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http://dx.doi.org/10.1002/bdr2.1867DOI Listing
January 2021

Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jan 7;1162:122477. Epub 2020 Dec 7.

Department of Chemistry, Hacettepe University, Ankara, Turkey. Electronic address:

Boronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic column for the recognition of cis-diol containing adenosine nucleoside from its analogue molecule of deoxyadenosine triphosphate, dATP, non-cis-diol containing compound was investigated both by UV and HPLC studies. When the relative selectivity coefficients are compared, the [POSS-MAH-PBA] boronate affinity monolithic column is 4.25 times more selective for adenosine than [POSS-MAH] monolithic column. Besides, to determine endogenous nucleosides in biological fluids, which may serve as non-invasive cancer biomarkers, nucleosides were spiked into the urine solutions and passed through the [POSS-MAH-PBA] boronate affinity monolithic column, and the nucleosides were confirmed by HPLC. The adenosine recognition capability of the [POSS-MAH-PBA] boronate affinity monolithic column with an average enrichment factor of 48.9-fold was apparently superior to that of the [POSS-MAH] monolithic column. Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) with nano-sized stable 3-dimensional architectures provided the advantage of being used as an adsorbent for the monolithic structure by providing high surface area, 507.60 m/g, and enabling vinyl groups to function with amino acid-based MAH monomers capable of providing electrons to coordinate PBA. Recovery results of more than 90% for adenosine showed that the [POSS-MAH-PBA] boronate affinity monolithic column could be a promising adsorbent for selective adsorption of cis-diol containing compounds such as nucleosides.
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http://dx.doi.org/10.1016/j.jchromb.2020.122477DOI Listing
January 2021

Lymphocyte Subset Alteration and Monocyte CD4 Expression Reduction in Patients with Severe COVID-19.

Viral Immunol 2020 Nov 23. Epub 2020 Nov 23.

Medical Biochemistry Laboratory, Ministry of Health Ankara City Hospital, Ankara, Turkey.

The spectrum of coronavirus disease 2019 (COVID-19) severity, related to cellular immune functions, has not been fully clarified yet. Therefore, this study aimed to investigate the alteration of peripheral blood cells in patients with COVID-19. The flow cytometric characterization of immune cell subset was performed on 69 COVID-19 patients and 21 healthy controls. These data were evaluated based on the disease severity. A total of 69 patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were classified as asymptomatic infection ( = 14), nonsevere ( = 39), and severe ( = 16) groups. Decreased lymphocytes and increased CD14 + 4- monocytes are found in patients with severe COVID-19. Decreased CD4 expression level was observed in the monocytes of patients with severe COVID-19. The total lymphocytes, B and T lymphocytes, CD4+ cells and CD8+ cells, and natural killer (NK) and natural killer T (NKT) cells were found to be decreased in patients with severe COVID-19. The CD4+/CD8+ ratio was not significantly different between patients with COVID-19 and healthy controls. The percentage of activated T cells (CD3+HLA-DR+) and B cells (CD19+CD38+) was lower in patients with severe COVID-19. Age and CD4- monocytes were independent predictors of disease severity. The SARS-CoV-2 infection may affect lymphocyte subsets, resulting in decreased T and B cells, monocytes, and NK and NKT cells. Decreased CD4 expression level by monocytes was significantly correlated with disease severity. Further studies on the host immune response to SARS-CoV-2 infection are necessary to predict the disease severity and protect against the virus.
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http://dx.doi.org/10.1089/vim.2020.0166DOI Listing
November 2020

Evaluation of the efficiency of serum biotinidase activity as a newborn screening test in Turkey.

J Pediatr Endocrinol Metab 2021 Jan 13;34(1):89-94. Epub 2020 Nov 13.

Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey.

Objectives: Biotinidase Deficiency (BD) is an autosomal recessive metabolic disorder. However, the relationship between genotype and biochemical phenotype has not been completely elucidated yet. But still, some mutations are accepted to be associated with profound or partial deficiency. We aimed to evaluate the results of biochemical enzyme activity in accordance with the presence of genetic mutations and investigate the correlation between genotype and biochemical phenotype together in the study.

Methods: This retrospective study was carried out using data from medical records of 133 infants detected by the newborn screening followed by serum biotinidase activity (BA) detection with semi-quantitative colorimetric method. Mutation analysis was performed to confirm the diagnosis. In addition, the expected biochemical phenotype based on the known mutant alleles were compared with the observed biochemical phenotype.

Results: When confirmed with mutation analysis results, the diagnostic sensitivity and specificity of serum BA with spectrophotometric method was 93.1% and 95.1%, respectively. In 93.98% of the cases conformity was observed between the biochemical phenotype and the genotype. The c.1330 G>C(p.D444H) and c.470 G>A (p.Arg157His) were the most common allelic variants with frequencies of 63.69% and 33.75%, respectively.

Conclusions: The diagnostic test is supposed to have a high sensitivity to identify asymptomatic BD patients. Apparently healthy cases with almost normal enzyme activity and a variant allele in the genetic analysis were reported to present symptoms under stress conditions, which should be kept in mind. This study can be accepted as an informative report as it may contribute to the literature in terms of the allelic frequency and determination of the relation between genotype and biochemical phenotype. Also, method verification including the assessment of possible effects of non-genetic factors on BA according to the certain mutation types is warranted.
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http://dx.doi.org/10.1515/jpem-2020-0382DOI Listing
January 2021

Ocular Surface and Conjunctival Cytology Findings in Patients With Confirmed COVID-19.

Eye Contact Lens 2021 Apr;47(4):168-173

Department of Ophthalmology (E.B., E.M.), Faculty of Medicine, Kafkas University, Kars, Türkiye; Department of Ophthalmology (S.Ö.), Okan University, Istanbul, Türkiye; and Departments of Pathology (F.Y.), and Infectious Disease and Clinical Microbiology (O.C.), Harakani State Hospital, Kars, Türkiye.

Purpose: To assess the effect of severe acute respiratory syndrome coronavirus-2 infection on the conjunctiva and tear film.

Methods: Thirty-eight patients with confirmed COVID-19 and 31 healthy controls were included in this prospective and observational study. Individuals with COVID-19 formed the patient group, and healthy individuals formed the control group. Conjunctival impression cytology (CIC), TBUT, Schirmer II test, and ocular surface disease index were evaluated in all participants.

Results: No significant difference was observed regarding the mean age and gender between the groups (P=0.786 and P=0.122, respectively). The mean TBUT and Schirmer II test results did not differ between the two groups (P=0.496 and P=0.447, respectively). The CIC results revealed decreased density and cell size of goblet cells and moderate to high enlargement, squamous changes, and increased nucleocytoplasmic ratio in nongoblet epithelial cells in the COVID-19 group compared with the control group. Based on the Nelson classification in CIC samples, 60.6% of the COVID-19 group and 19.4% of the control group had changes consistent with grade 2 or above. The presence of neutrophils in CIC was significantly higher in the COVID-19 group (P<0.001), whereas the presence of lymphocyte was similar between the two groups (P=0.247).

Conclusion: This study revealed the pathological conjunctival alterations in patients with COVID-19 and demonstrated that pathological ocular surface alterations may present even at the beginning of COVID-19 without clinically significant ocular manifestation.
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http://dx.doi.org/10.1097/ICL.0000000000000752DOI Listing
April 2021

Convalescent plasma therapy in patients with COVID-19.

Transfus Apher Sci 2021 Feb 19;60(1):102955. Epub 2020 Sep 19.

Republic of Turkey, Ministry of Health, Public Hospitals General Directorate, Ankara, Turkey. Electronic address:

Introduction: Passive antibody therapy has been used to immunize vulnerable people against infectious agents. In this study, we aim to investigate the efficacy of convalescent plasma (CP) in the treatment of severe and critically ill patients diagnosed with COVID-19.

Method: The data of severe or critically ill COVID-19 patients who received anti-SARS-CoV-2 antibody-containing CP along with the antiviral treatment (n = 888) and an age-gender, comorbidity, and other COVID-19 treatments matched severe or critically ill COVID-19 patients at 1:1 ratio (n = 888) were analyzed retrospectively.

Results: Duration in the intensive care unit (ICU), the rate of mechanical ventilation (MV) support and vasopressor support were lower in CP group compared with the control group (p = 0.001, p = 0.02, p = 0.001, respectively). The case fatality rate (CFR) was 24.7 % in the CP group, and it was 27.7 % in the control group. Administration of CP 20 days after the COVID-19 diagnosis or COVID-19 related symptoms were associated with a higher rate of MV support compared with the first 3 interval groups (≤5 days, 6-10 days, 11-15 days) (p=0.001).

Conclusion: CP therapy seems to be effective for a better course of COVID-19 in severe and critically ill patients.
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http://dx.doi.org/10.1016/j.transci.2020.102955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501849PMC
February 2021

Clinical and laboratory features of COVID-19: Predictors of severe prognosis.

Int Immunopharmacol 2020 Nov 9;88:106950. Epub 2020 Sep 9.

Department of Medical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara City Hospital, 06800 Ankara, Turkey.

Background: Coronavirus disease 2019 (COVID-19) emerged first in December 2019 in Wuhan, China and quickly spread throughout the world. Clinical and laboratory data are of importance to increase the success in the management of COVID-19 patients.

Methods: Data were obtained retrospectively from medical records of 191 hospitalized patients diagnosed with COVID-19 from a tertiary single-center hospital between March and April 2020. Prognostic effects of variables on admission among patients who received intensive care unit (ICU) support and those who didn't require ICU care were compared.

Results: Patients required ICU care (n = 46) were older (median, 71 vs. 43 years), with more underlying comorbidities (76.1% vs. 33.1%). ICU patients had lower lymphocytes, percentage of large unstained cell (%LUC), hemoglobin, total protein, and albumin, but higher leucocytes, neutrophils, neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), platelet-lymphocytes ratio (PLR), urea, creatinine, aspartate amino transferase (AST), lactate dehydrogenase (LDH), and D-dimer when compared with non-critically ill patients (p < 0.001). A logistic regression model was created to include ferritin, %LUC, NLR, and D-dimer. %LUC decrease and D-dimer increase had the highest odds ratios (0.093 vs 5.597, respectively) to predict severe prognosis. D-dimer, CRP, and NLR had the highest AUC in the ROC analysis (0.896, 0.874, 0.861, respectively).

Conclusions: The comprehensive analysis of clinical and admission laboratory parameters to identify patients with severe prognosis is important not only for the follow-up of the patients but also to identify the pathophysiology of the disease. %LUC decrease and D-dimer, NLR, and CRP increases seem to be the most powerful laboratory predictors of severe prognosis.
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http://dx.doi.org/10.1016/j.intimp.2020.106950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480980PMC
November 2020

Clinical characteristics and neonatal outcomes of liveborn newborns with hydrops fetalis treated in a tertiary level neonatal intensive care unit.

Birth Defects Res 2020 04;112(6):515-522

Department of Neonatology, Necmettin Erbakan University, Meram Medical Faculty, Konya, Turkey.

Background: This study was performed for examining the neonatal results and aetiological factors of neonates with hydrops fetalis (HF) and determining the factors affecting mortality.

Methods: The medical records of liveborn neonates with HF who were admitted to a tertiary Neonatal Intensive Care Unit (NICU) in Konya, Turkey, between 2013 and 2019 were reviewed retrospectively. The demographic data, prenatal intervention, clinical findings, and results of the patients were recorded.

Results: A total of 32.6% of the 46 liveborn HF infants had immune HF (IHF), while 67.4% had nonimmune HF (NIHF); there was prenatal diagnoses in 39 (84.7%) cases. Cordocentesis and blood transfusion (n = 14; 30.4%) were the prenatal diagnosis and treatment interventions with the highest rate. A total of 16 patients (34.7%) received in utero interventional treatment. It was determined that the mean gestational age was not associated with mortality; moreover, birthweight (BW), Apgar score and the need for mechanical ventilation affected mortality.

Conclusion: The prognosis changes according to different etiologies of HF. However, despite the developments in neonatal care, mortality is still high in HF infants.
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http://dx.doi.org/10.1002/bdr2.1640DOI Listing
April 2020

A CLOCK-binding small molecule disrupts the interaction between CLOCK and BMAL1 and enhances circadian rhythm amplitude.

J Biol Chem 2020 03 4;295(11):3518-3531. Epub 2020 Feb 4.

Department of Molecular Biology and Genetics, Koc University, Rumelifeneri Yolu, Sariyer, Istanbul, Turkey; Department of Chemical and Biological Engineering, Koc University, Rumelifeneri Yolu, Sariyer, Istanbul, Turkey. Electronic address:

Proper function of many physiological processes requires a robust circadian clock. Disruptions of the circadian clock can result in metabolic diseases, mood disorders, and accelerated aging. Therefore, identifying small molecules that specifically modulate regulatory core clock proteins may potentially enable better management of these disorders. In this study, we applied a structure-based molecular-docking approach to find small molecules that specifically bind to the core circadian regulator, the transcription factor circadian locomotor output cycles kaput (CLOCK). We identified 100 candidate molecules by virtual screening of ∼2 million small molecules for those predicted to bind closely to the interface in CLOCK that interacts with its transcriptional co-regulator, Brain and muscle Arnt-like protein-1 (BMAL1). Using a mammalian two-hybrid system, real-time monitoring of circadian rhythm in U2OS cells, and various biochemical assays, we tested these compounds experimentally and found one, named CLK8, that specifically bound to and interfered with CLOCK activity. We show that CLK8 disrupts the interaction between CLOCK and BMAL1 and interferes with nuclear translocation of CLOCK both and Results from further experiments indicated that CLK8 enhances the amplitude of the cellular circadian rhythm by stabilizing the negative arm of the transcription/translation feedback loop without affecting period length. Our results reveal CLK8 as a tool for further studies of CLOCK's role in circadian rhythm amplitude regulation and as a potential candidate for therapeutic development to manage disorders associated with dampened circadian rhythms.
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http://dx.doi.org/10.1074/jbc.RA119.011332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076206PMC
March 2020

The Effect of Kangaroo Mother Care, Provided in the Early Postpartum Period, on the Breastfeeding Self-Efficacy Level of Mothers and the Perceived Insufficient Milk Supply.

J Perinat Neonatal Nurs 2020 Jan/Mar;34(1):80-87

Department of Children Health and Diseases Nursing, Faculty of Nursing, Çanakkale Onsekiz Mart University, Turkey (Dr Yilmaz and Mr Oğul); Department of Children Health and Diseases Nursing, Faculty of Nursing, Selcuk University, Konya, Turkey (Dr Küçükoglu); Department of Nursing, Faculty of Health Sciences, Istanbul Medeniyet University, Istanbul, Turkey (Dr Aytekin Ozdemir); and Çanakkale Obstetrics and Gynecology Clinic, Health Practice and Research Hospital, Turkey (Ms Aski).

The aim of this study was to determine the effect of kangaroo mother care, provided in the early postpartum period, on the breastfeeding self-efficacy level and the perceived insufficient milk supply. This study was conducted as the quasi-experimental design. The population of the study consisted of the mothers and their infants, to whom they gave birth in a university hospitals located in either eastern or western Turkey, between December 2016 and June 2017. In this study, mothers and their infants were randomly assigned to the experimental group (kangaroo mother care, n = 30) and the control group (n = 30). This study included 2500 to 4000 g birth weight infants who had no serious health problems and no sucking problems. The Introductory Information Form, the Breastfeeding Self-Efficacy Scale, and the Perception of Insufficient Milk Questionnaire were used to collect the data. In this study, kangaroo mother care was provided as a nursing intervention for the mothers in the experimental group twice a day until they were discharged. Any other application was not performed in the control group's mothers apart from the routine application. Ethical principles were adhered in all stages of the study. The breastfeeding self-efficacy mean score (65.50 ± 3.95) of the mothers who performed kangaroo mother care was higher than the mean score of the mothers who did not perform kangaroo mother care (55.50 ± 7.00) (P < .001). In addition, mothers in the experimental group (46.60 ± 3.40) perceived their milk more sufficiently than mothers in the control group (30.17 ± 11.37) (P < .001). In the study, a statistically significant correlation was determined between breastfeeding self-efficacy levels of mothers in the experimental group and the perceived insufficient milk supply (P < .05). In the study, kangaroo mother care increased breastfeeding self-efficacy perception of the mothers and reduced the perceived insufficient milk supply. This shows that kangaroo mother care can potentially have an important effect on breastfeeding perceptions.
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http://dx.doi.org/10.1097/JPN.0000000000000434DOI Listing
November 2020

Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography.

J Chromatogr Sci 2019 Aug;57(8):758-765

Department of Chemistry, Hacettepe University, Beytepe, 06800, Ankara, Turkey.

The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10-6 m2 V-1 s-1 and 7.60 × 10-6 m2 V-1 s-1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.
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http://dx.doi.org/10.1093/chromsci/bmz047DOI Listing
August 2019

Mosaic trisomy 14 and aorta-pulmonary window association: A case report.

Turk Kardiyol Dern Ars 2019 Jun;47(4):319-323

Necmettin Erbakan University Meram Medical Faculthy, Department of Neonatology, Konya, Turkey.

Trisomy 14 mosaicism is a rare chromosomal abnormality with distinct and recognizable clinical features. Congenital heart anomalies can accompany in this syndrome. To the best of our knowledge, this is the first case of mosaic trisomy 14 with an aortopulmonary window to be described in the literature.
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http://dx.doi.org/10.5543/tkda.2018.25273DOI Listing
June 2019

Novel QCM and SPR sensors based on molecular imprinting for highly sensitive and selective detection of 2,4-dichlorophenoxyacetic acid in apple samples.

Mater Sci Eng C Mater Biol Appl 2019 Sep 23;102:483-491. Epub 2019 Apr 23.

Dicle University, Faculty of Science, Department of Chemistry, Diyarbakır, Turkey.

This study aims to develop molecularly imprinted based quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) sensors for highly sensitive and selective detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and to determine their accuracy and precision by liquid chromatography-tandem mass spectrometry (LC-MS/MS) as a reference technique. Here, we synthesized non-imprinted (NIP) and 2,4-D-imprinted (MIP) [ethylene glycol dimetacrylate-N-metacryloyl-(l)-tryptophan methyl ester-p(EGDMA-MATrp)] polymeric nanofilms by using molecular imprinting technique. MIP and NIP nanofilms were characterized by fourier transform infrared spectroscopy attenuated total reflectance (FTIR-ATR), atomic force microscope (AFM), contact angle and ellipsometer measurements. The molecular imprinting procedures were successfully carried out and it was found that the prepared polymeric surfaces were highly desirable for sensitive recognition by QCM and SPR sensors. Competitive experiments for the sensors revealed that MIP nanofilms were found to show more sensitivity and selectivity than NIP ones. The sensor responses have a good linear relationship with 2,4-D concentrations in the range of 0.23-8.0 nM with a limit of detection at 20.17 ng/L for QCM and 24.57 ng/L for SPR sensors. In conclusion, both QCM and SPR sensor systems showed good accuracy and precision, with recovery percentages between 90 and 92% and 87-93%, respectively. Furthermore, they have a fast response time, reusability, high selectivity and sensitivity and low limit of detection.
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http://dx.doi.org/10.1016/j.msec.2019.04.056DOI Listing
September 2019

Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection.

J Biomater Sci Polym Ed 2019 06 7;30(8):646-661. Epub 2019 Apr 7.

a Faculty of Science, Department of Chemistry , Hacettepe University , Ankara , Turkey.

Human serum albumin (HSA) is a major blood plasma protein also found in urine where its existence may be a marker of some types of liver or kidney dysfunction. Herein, we fabricated a novel surface plasmon resonance (SPR) nanosensor for selective, sensitive, and label-free microalbumin detection both in aqueous and urine sample solutions. First, HSA-imprinted nanoparticles were synthesized, which consist of ethylene glycol dimethacrylate and N-methacryloyl-L-leucine methyl ester as a cross-linker and functional monomer. The nanoparticles were characterized by zeta-size and scanning electron microscope analyses and were dropped onto the SPR chip surface to make HSA sensitive nanosensor. Characterization studies of HSA-imprinted SPR chip were carried out by atomic force microscopy, Fourier-transform infrared spectroscopy, contact angle, and ellipsometer. The limit of detection and limit of quantification values of HSA-imprinted SPR nanosensor were calculated as 0.7 pM and 1.9 pM for the concentration range of 0.15-500 nM. Selectivity studies of HSA-imprinted SPR nanosensor were achieved with hemoglobin and transferrin proteins which were chosen as competitor molecules. HSA-imprinted SPR nanosensor was displayed highly selective and sensitive to HSA.
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http://dx.doi.org/10.1080/09205063.2019.1600181DOI Listing
June 2019

Phenol removal from wastewater by surface imprinted bacterial cellulose nanofibres.

Environ Technol 2020 Oct 1;41(24):3134-3145. Epub 2019 May 1.

Department of Chemistry, Hacettepe University, Ankara, Turkey.

In this study, we have reported a novel wastewater treatment technique by phenol imprinted bacterial cellulose (BC-MIP) nanofibres with high specificity and adsorption capacity. -methacryloyl-(L) phenylalanine methyl ester (MAPA) functional monomer was used to create specific binding sites for the template molecule phenol via electrostatic and hydrophobic interactions. BC-MIP nanofibres were synthesized by surface imprinting approach in the presence of different amounts of total monomer (% weight), monomer/template ratio and polymerization time. Then, the nanofibres were characterized by FTIR-ATR, surface area analysis (BET), elemental analysis, scanning electron microscopy (SEM) and contact angle measurements. Adsorption studies were performed with respect to pH, temperature and ionic strength, and the adsorption capacity was calculated by using the spectrophotometer. In order to desorb the adsorbed phenol from BC-MIP nanofibres, 0.1 M NaCl solution was used. Besides, BC-MIP nanofibres were applied to real wastewater samples from Ergene basin in Turkey. The suitable equilibrium isotherm was determined as Langmuir isotherm. To evaluate the selectivity of the BC-MIP nanofibres, similar molecules were utilized as competitor molecules, which were 2-chlorophenol, 4-chlorophenol and 2,4-dichlorophenol. Electrostatic interactions were found to contribute to the generation of specific recognition binding sites. The results have shown that imprinting of phenol was achieved successfully with high adsorption capacity. The phenol removal efficiency was reported up to 97%. BC-MIP nanofibres were used 10 times with a negligible decrease in adsorption capacity.
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http://dx.doi.org/10.1080/09593330.2019.1600043DOI Listing
October 2020

Glanzmann Thrombasthenia in a Newborn with Heterozygous Factor V Leiden and Heterozygous MTHFR C677T Gene Mutations.

Indian Pediatr 2019 02;56(2):143-144

Department of Pediatric Hematology and Oncology, Necmettin Erbakan University Meram Medical Faculthy, Konya, Turkey.

Introduction: Glanzmann thrombasthenia is a rare congenital platelet dysfunction.

Case Characteristics: A 2-day-old male neonate delivered at 35 weeks' gestation was referred with extensive bruising and jaundice. His elder sibling had Glanzmann thrombasthenia, and his mother had thrombophilic risk factors. Flow cytometric analysis revealed absent CD41/CD61. A molecular thrombophilia panel revealed the presence of heterozygous factor V Leiden G1691A and methylenetetrahydrofolate reductase C677T gene mutations.

Outcome: General precautions to avoid injuries and spontaneous bleeding were advised.

Message: Life-threatening bleeding may not be the first finding in cases of thrombasthenia accompanied by thrombophilic risk factors.
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February 2019

Arsenic-induced inflammation in workers.

Mol Biol Rep 2019 Apr 19;46(2):2371-2378. Epub 2019 Feb 19.

Department of Medical Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey.

Occupational and environmental exposures to metal and metalloids can result in neurotoxicity and immunotoxicity. Selenium (Se) is essential for the proper functioning of neutrophils, macrophages, natural killer (NK) cells, T-lymphocytes and other immune mechanisms, while zinc (Zn) is a trace element essential for basic cell activities, including cell growth and differentiation. Arsenic (As) may lead to different types of immunosuppressive effects. This study consisted of 62 male workers, who had been exposed to arsenic for different durations and 73 non-exposed male workers (control group) with no history of occupational toxic metal exposure. Whole blood and serum samples were taken from each participant for immunological, toxicological and routine analysis during their annual periodical examination. Arsenic, selenium and zinc levels were determined by the ICP-MS and cytokines, IL-6, IL-10, TNF-α, sE-selectin and VCAM-1, were measured by ELISA. There were statistically significant differences (p < 0.001) between control and As-exposed group in As (1.37 ± 0.42 vs. 4.27 ± 1.54 µg/L) and Se levels (106.37 ± 48.04 vs. 74.70 ± 30.45 µg/L). The changing levels of As, Zn and Se seems to affect the severity of inflammatory reactions based on IL-6, IL-10 and TNF-α levels (r = 0.755, r = 0.679 and r = 0.617, respectively, for all p < 0.01). Selenium was found to have a suppressive effect on cytokines, as evidenced by Pearson correlations and regression analysis. These findings support the need to closely monitor Se levels in individuals exposed to arsenic and benefits for Se supplementation in the case of arsenic exposure, occupationally or environmentally.
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http://dx.doi.org/10.1007/s11033-019-04694-xDOI Listing
April 2019

Intrauterine volvulus that had been misunderstood as an antenatal mesenteric cyst.

Pediatr Neonatol 2019 08 4;60(4):467-468. Epub 2018 Sep 4.

Necmettin Erbakan University, Meram Medical Faculty, Department of Pediatrics, Division of Neonatology, Meram, Konya, Turkey.

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http://dx.doi.org/10.1016/j.pedneo.2018.08.009DOI Listing
August 2019

Protein depletion with bacterial cellulose nanofibers.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Nov 29;1099:1-9. Epub 2018 Aug 29.

Hacettepe University, Department of Chemistry, Beytepe, Ankara, Turkey. Electronic address:

In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications. Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions. MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature. The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements. The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system. The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo). The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively. In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis. As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.
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http://dx.doi.org/10.1016/j.jchromb.2018.08.030DOI Listing
November 2018

A Randomized, Controlled, Split-mouth Trial Evaluating the Clinical Performance of High-viscosity Glass-ionomer Restorations in Noncarious Cervical Lesions: Two-year Results.

J Adhes Dent 2018 ;20(4):299-305

Purpose: To compare the two-year clinical performance of high-viscosity glass ionomer (Hv-GIC) restorations in noncarious cervical lesions with nanohybrid composite restorations applied with a three-step etch-and-rinse adhesive (E&Ra/nanoC).

Materials And Methods: One hundred thirty-four noncarious cervical lesions were included and assigned to two groups according to the split-mouth design. The cervical lesions in the experimental group were restored with an Hv-GIC (Equia, GC), while a nanohybrid composite (G-aenial, GC) with a three-step etch-and-rinse adhesive (Optibond FL, Kerr) was applied as the control. All tested restorative materials were used according to the manufacturers' instructions. Clinical evaluation was performed after one week, six months, one year, and two years using World Dental Federation criteria. Data were analyzed using Friedman's ANOVA and Mann-Whitney U-tests (α = 0.05).

Results: After two years, Hv-GIC restorations had a retention rate of 91% in comparison to 100% for E&Ra/nanoC restorations. Significant differences existed between the two restorative materials solely with respect to the retention parameter after two years (p = 0.008).

Conclusions: The two-year clinical performance of Hv-GIC was clinically acceptable. However, the retention of E&Ra/nanoC restorations was significantly better than that of Hv-GIC restorations after two years.
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http://dx.doi.org/10.3290/j.jad.a40985DOI Listing
January 2019

Serum asymmetric dimethylarginine and nitric oxide levels in Turkish patients with acute ischemic stroke.

Adv Clin Exp Med 2019 May;28(5):693-698

Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazit University, Ankara, Turkey.

Background: Nitric oxide synthase (NOS) is present in the brain and cerebral arteries and it enables the synthesis of nitric oxide (NO), which plays a critical role in brain perfusion. Asymmetrical dimethylarginine (ADMA) is an endogenous NOS inhibitor.

Objectives: The aim of this study was to evaluate serum ADMA levels, which are an indicator of endothelial dysfunction of the renal functions in patients with acute ischemic stroke, and to determine whether there is a possible correlation between ADMA and NO levels and the l-arginine-to-ADMA ratio.

Material And Methods: Fifty-two patients (22 male and 30 female; mean age: 75.2 ±10.1 years) with a diagnosis of acute ischemic stroke in the first 24 h post-stroke and 48 healthy individuals (controls; 13 male and 35 female; mean age: 60.1 ±7.92 years) were included in this study. The risk factors recorded and evaluated were age and gender of the patients, serum lipid levels, serum ADMA levels, nitrate-to-nitrite ratios, l-arginine, l-arginine-to-ADMA ratios, sedimentation rate, C-reactive protein (CRP), urea and creatinine levels, and glomerular filtration ratio (eGFR).

Results: The mean serum ADMA level was 0.48 ±0.23 μM for the patients and 0.36 ±0.18 μM for the controls. The mean NO level was 2.78 ±0.59 μM for the patient group and 4.49 ±2.84 μM for the controls. The ADMA levels for the patient group were significantly higher than for the control group (p = 0.011); the NO levels for the patients were significantly lower than for the controls (p < 0.001). The logistic regression method demonstrated that ADMA and NO levels may be independent risk factors for the patient group, and the receiver operating characteristic (ROC) curve analysis showed that both of these variables were discriminative risk factors.

Conclusions: An increased serum level of the NOS inhibitor ADMA was found to be a possible independent risk factor for ischemic stroke.
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http://dx.doi.org/10.17219/acem/78360DOI Listing
May 2019

Hemodilution is not the only reason of difference: Comparison of fasting and non-fasting lipoproteins in paired samples.

Clin Biochem 2018 Nov 25;61:28-33. Epub 2018 Aug 25.

Ankara Yıldırım Beyazıt University, Department of Medical Biochemistry, Ankara, Turkey.

Introduction: Low-density lipoprotein cholesterol (LDL) is an important risk factor for cardiovascular disease (CVD) and generally measured after 8-12 h fasting. However, some recent studies have pointed that non-fasting lipoproteins, especially LDL concentrations, are better indicators for demonstrating CVD risk and atherosclerosis. They asserted that nutrition is a negligible factor on changes in lipoprotein concentrations and claimed this difference as a result of hemodilution effect, caused from fluid intake and can be eliminated by applying some adjustments. We aimed to compare the fasting and non-fasting LDL values of the same individuals and discuss whether non-fasting and fasting LDL results can be used in place of each other, directly or after applying hemodilution correction models.

Material And Methods: Fasting and non-fasting blood samples of 248 apparently healthy participants were collected. Lipid panel tests, albumin and hemoglobin levels were studied in each sample. Results were evaluated in seven different models which were recommended to correct the hemodilution effect on fasting and non-fasting lipid concentrations of the same individual. Concordance of fasting and non-fasting risk group of the individual were calculated according to the National Cholesterol Education Program classification.

Results: Fasting and non-fasting LDL and non-high density lipoprotein cholesterol (non-HDL) concentrations were significantly different in every model (p < 0.001). Concordance results of fasting and non-fasting LDL and non-HDL risk groups were 63.8% and 77.9% respectively.

Conclusions: Our results demonstrated that fasting and non-fasting LDL and non-HDL concentrations could not be used in place of each other even when the results were adjusted for elimination of the hemodilution effect.
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http://dx.doi.org/10.1016/j.clinbiochem.2018.08.009DOI Listing
November 2018

Three-year clinical evaluation of high-viscosity glass ionomer restorations in non-carious cervical lesions: a randomised controlled split-mouth clinical trial.

Clin Oral Investig 2019 Mar 17;23(3):1473-1480. Epub 2018 Aug 17.

Department of Restorative Dentistry, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.

Objective: To compare the 3-year clinical performance of high-viscosity glass ionomer restorations with that of composite restorations in non-carious cervical lesions (NCCLs).

Materials And Methods: One hundred thirty-four NCCLs were randomised into two groups according to a split-mouth design. In the experimental group (Hv-GIC), lesions were restored with a high-viscosity glass ionomer (EQUIA Fil, GC), whereas a composite resin (G-aenial, GC) was applied in the control group (E&Ra/comp). All restorative materials were used according to the manufacturers' instructions. Clinical evaluations were performed after 1 week, 6 months, 1 year, 2 years, and 3 years using FDI (World Dental Federation) criteria. Data were analysed using Friedman's ANOVA and Mann-Whitney U tests (α = 0.05).

Results: While retention rates of the Hv-GIC group were 98.5%, 96%, 91%, and 87% in respective evaluation periods, no retention loss was observed in the E&Ra/comp group at any time. There was a statistically significant difference between study groups regarding the retention criterion in both the second and third years (p = 0.008 and p = 0.003, respectively). Furthermore, there was a statistically significant difference between the groups in terms of surface lustre at the third-year recall, in favour of the E&Ra/comp group (p = 0.022).

Conclusions: The 3-year clinical performance of E&Ra/comp restorations in NCCLs was better than that of Hv-GIC restorations. The most common problems in Hv-GIC restorations were a loss of retention and reduction in surface lustre.

Clinical Relevance: Although the 3-year clinical performance of Hv-GIC restorations in non-retentive lesions was acceptable, it was worse than in composites. The operator should consider the benefit/loss ratio of Hv-GIC when he/she decides to use this material in non-retentive cavities, especially those that are shallow.
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http://dx.doi.org/10.1007/s00784-018-2575-yDOI Listing
March 2019

Netrin 1 and Alpha-Methyl Acylcoenzim-A Racemase in diagnosis of prostate cancer.

Colomb Med (Cali) 2018 Jun 30;49(2):164-168. Epub 2018 Jun 30.

Ankara Numune Research and Training Hospital, Department of Urology. Ankara, Turkey.

Objectives: To investigate serum and urine levels of Alpha-methylacyl-CoA-racemase (AMACR) and Netrin 1 in patients with and without prostate cancer and to determine whether these markers could be used as alternatives in diagnosis of prostate cancer instead of serum prostate specific antigen (PSA) levels.

Methods: One hundred and seventy five patients between 45-75 years to whom transrectal ultrasound guided biopsies were performed for abnormal serum PSA levels or digital rectal examinations were included. The levels of AMACR and Netrin 1 levels of blood and urine samples of 5 mL those were taken prior to biopsies were measured. .

Results: The mean age of the patients was 62.7 ±6.4 years. Prostate cancer was detected in 40 patients (22.8%) while 135 of them (77.2%) were diagnosed as benign prostate hyperplasia (BPH). In BPH group, serum and urine levels of AMACR and Netrin 1 were 13.4 ±16.9 ng/mL; 7.1 ±3.4 ng/mL; 164.1±46 pg/mL and 19.5 ±5.0 pg/mL respectively. The levels of serum and urine levels of AMACR and Netrin 1 were 10.2 ±9.8 ng/mL; 6.8 ±2.5 ng/mL; 159.1 ±44.1 pg/mL and 20.1 ±5.3 pg/mL respectively in prostate cancer group. There was no statistically significant difference or correlation between these two groups serum and urine AMACR and Netrin 1 results.

Conclusions: Serum and urine levels of AMACR and Netrin 1 were not found to be alternatives for serum PSA levels in the diagnosis of prostate cancer in this study.
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http://dx.doi.org/10.25100/cm.v49i2.3011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084917PMC
June 2018