Publications by authors named "Fatih Sen"

148 Publications

Green synthesis, characterization and bioactivity of biogenic zinc oxide nanoparticles.

Environ Res 2021 Aug 18;204(Pt A):111897. Epub 2021 Aug 18.

Sen Research Group, Department of Biochemistry, University of Dumlupinar, 43000, Kutahya, Turkey. Electronic address:

In this study, we tried to enlighten the structure of zinc oxide nanoparticles (ZnO NPs) obtained from Thymbra Spicata L. plant by using green synthesis method in various ways. Some properties of zinc oxide nanoparticles were determined by using the characterization methods that scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDX), fouirer transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet visible spectroscopy (UV-Vis) spectroscopy methods. The detected Zn nanoparticle sizes were determined to be between 6.5 nm and 7.5 nm. In addition to these studies, we investigated the antimicrobial effects of zinc oxide nanoparticles obtained by green synthesis against some pathogens. According to the results, it was seen that zinc oxide nanoparticles formed zones with a diameter of 16.3 mm, 10.25 mm, 13 mm and 10.2 mm, respectively, against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 bacteria and Candida albicans ATTC 90028 fungus, respectively. However, the radical quenching activity (DPPH) of the nanoparticles (Ts-ZnONP (79.67%)) was determined to be quite good compared to the positive control BHA. In addition, it is seen that the protective effect of ZnO NPs against DNA damage increases depending on the concentration. At a concentration of 100 mg/L, the DNA damage inhibitory effect was found to be maximum. In line with the comprehensive results, it was determined that the zinc oxide nanoparticles obtained with the green synthesis method have the potential of use in a wide variety of fields.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2021.111897DOI Listing
August 2021

Chemoselective hydrogenation of aromatic nitro compounds in the presence of homogeneous Pd based catalysts.

Chemosphere 2021 Nov 27;282:130887. Epub 2021 May 27.

Sen Research Group, Department of Biochemistry, Dumlupınar University, 43100, Kütahya, Turkey. Electronic address:

The chemoselective reduction of aromatic nitro compounds to amine derivatives was easily performed with remarkable yields under ultrasonic conditions in a HO/MeOH mixture (v/v = 1/4). In this process, commercially available BINAP.PdCl and NaBH were used as homogenous catalysts and the hydrogen source, respectively. The developed method has a high economic value and can be adapted to the industry. A variety of nitroarene derivatives were reacted by undergoing the BINAP.PdCl catalyzed reduction reaction. All nitroarenes were selectively hydrogenated to aromatic primary amines with quantitative yields in 15 min. The obtained primary amines were determined by H and C nuclear magnetic resonance spectroscopy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130887DOI Listing
November 2021

Enhanced electrochemical performance of MnNiO/rGO nanocomposite as pseudocapacitor electrode material and methanol electro-oxidation catalyst.

Nanotechnology 2021 May 21;32(32). Epub 2021 May 21.

Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey.

Binary transition metal oxides with encouraging electrocatalyst properties have been suggested as electrode materials for supercapacitors and methanol oxidation. Hence, in this work, a binary mixed metal oxide based on nickel and manganese (MnNiO) and its hybrid with reduced graphene oxide were synthesized by a one-step hydrothermal method. After physical and morphological characterization, the potential of these nanostructures was investigated for use as supercapacitor electrodes and methanol electro-oxidation. The results of the electrochemical analysis showed a substantial effect of adding rGO to the MnNiO. The MnNiO/rGO hybrid electrode supercapacitor exhibited good stability of 93% after 2000 consecutive CV cycles and a specific capacitance of 575 F gat the current density of 0.5 A g. Furthermore, the application of this hybrid nanomaterial in the methanol electro-oxidation reaction (MOR) indicated its appropriate electrochemical efficiency and stability in methanol oxidation. Our results show that MnNiO/rGO can be considered as a promising electrode material for energy applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/abfdedDOI Listing
May 2021

Corrigendum to "Cytotoxic effects of platinum nanoparticles obtained from pomegranate extract by the green synthesis method on the MCF-7 cell line" [Colloids Surf. B: Biointerfaces 163 (2018) 119-124].

Colloids Surf B Biointerfaces 2021 Aug 28;204:111780. Epub 2021 Apr 28.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2021.111780DOI Listing
August 2021

A critical review on the use of potentiometric based biosensors for biomarkers detection.

Biosens Bioelectron 2021 Jul 17;184:113252. Epub 2021 Apr 17.

Department of Chemical Engineering, American University of Sharjah, Sharjah, PO. Box 26666, United Arab Emirates.

Potentiometric-based biosensors have the potential to advance the detection of several biological compounds and help in early diagnosis of various diseases. They belong to the portable analytical class of biosensors for monitoring biomarkers in the human body. They contain ion-sensitive membranes sensors can be used to determine potassium, sodium, and chloride ions activity while being used as a biomarker to gauge human health. The potentiometric based ion-sensitive membrane systems can be coupled with various techniques to create a sensitive tool for the fast and early detection of cancer biomarkers and other critical biological compounds. This paper discusses the application of potentiometric-based biosensors and classifies them into four major categories: photoelectrochemical potentiometric biomarkers, potentiometric biosensors amplified with molecular imprinted polymer systems, wearable potentiometric biomarkers and light-addressable potentiometric biosensors. This review demonstrated the development of several innovative biosensor-based techniques that could potentially provide reliable tools to test biomarkers. Some challenges however remain, but these can be removed by coupling techniques to maximize the testing sensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2021.113252DOI Listing
July 2021

Editorial: Advances in Analytical Features of Electrochemical Methods for the Analysis of Complicated Real Samples.

Front Chem 2021 17;9:648920. Epub 2021 Mar 17.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupinar University, Evliya Çelebi Campus, Kütahya, Turkey.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.648920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010234PMC
March 2021

Plant Prebiotics and Their Role in the Amelioration of Diseases.

Biomolecules 2021 03 16;11(3). Epub 2021 Mar 16.

School of Bioengineering and Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan 173229, Himachal Pradesh, India.

Prebiotics are either natural or synthetic non-digestible (non-)carbohydrate substances that boost the proliferation of gut microbes. Undigested fructooligosaccharides in the large intestine are utilised by the beneficial microorganisms for the synthesis of short-chain fatty acids for their own growth. Although various food products are now recognized as having prebiotic properties, several others, such as almonds, artichoke, barley, chia seeds, chicory, dandelion greens, flaxseeds, garlic, and oats, are being explored and used as functional foods. Considering the benefits of these prebiotics in mineral absorption, metabolite production, gut microbiota modulation, and in various diseases such as diabetes, allergy, metabolic disorders, and necrotising enterocolitis, increasing attention has been focused on their applications in both food and pharmaceutical industries, although some of these food products are actually used as food supplements. This review aims to highlight the potential and need of these prebiotics in the diet and also discusses data related to the distinct types, sources, modes of action, and health benefits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom11030440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002343PMC
March 2021

Corrigendum for "Highly sensitive glucose sensor based on monodisperse palladium nickel/activated carbon nanocomposites" (Anal. Chim. Acta Volume 1010, 20 June 2018, Pages 37-43; 10.1016/j.aca.2018.01.035).

Anal Chim Acta 2021 Mar 22;1150:338219. Epub 2021 Jan 22.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2021.338219DOI Listing
March 2021

Corrigendum to "Investigation of the effect of pomegranate extract and monodisperse silver nanoparticle combination on MCF-7 cell line" [J. Biotechnol. 260 (2017) 79-83].

J Biotechnol 2021 Feb 2;328:115-116. Epub 2021 Feb 2.

Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2021.01.020DOI Listing
February 2021

Novel 1-butyl-3-methylimidazolium bromide impregnated chitosan hydrogel beads nanostructure as an efficient nanobio-adsorbent for cationic dye removal: Kinetic study.

Environ Res 2021 04 28;195:110809. Epub 2021 Jan 28.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.

In the present study, a novel 1-butyl-3-methylimidazolium bromide (BmImBr) impregnated chitosan beads were prepared and characterized using different methods, including XRD, FT-IR, EDX, SEM and BET. The FTIR analysis revealed that the BmImBr was successfully conjugated with the chitosan in the beads structure. The prepared beads were used as an efficient sorbent for the fast removal of methylene blue, as cationic dye model, from aqueous solution, whereas just 25 min was required to reach 86% removal efficiency. The increasing of BmImBr amount improved the adsorption performance of prepared beads. Also, it was found that the dye can be higher adsorbed on the beads surface by increasing the sorbent dosage and pH of solution, while the optimum dosage and pH were obtained 3 mg/L and 11, respectively. The kinetic study showed that the MB adsorption onto the CS-BmImBr beads follows the pseudo-fist order model and the intrinsic penetration controls the adsorption process. The properties of prepared chitosan- BmImBr IL conjugation confirmed that it can be exploited as an efficient adsorbent in the wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2021.110809DOI Listing
April 2021

Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II).

Anal Biochem 2021 03 28;617:114122. Epub 2021 Jan 28.

Department of Chemistry, Faculty of Science, Erciyes University, TR-38039 Kayseri, Turkey.

In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ab.2021.114122DOI Listing
March 2021

Electro-catalytic amplified sensor for determination of N-acetylcysteine in the presence of theophylline confirmed by experimental coupled theoretical investigation.

Sci Rep 2021 01 13;11(1):1006. Epub 2021 Jan 13.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.

The 1,l/-bis(2-phenylethan-1-ol)ferrocene, 1-butyl-3-methylimidazolium hexafluoro phosphate (BMPF6) and NiO-SWCNTs were used to modify carbon paste electrode (BPOFc/BMPF6/NiO-SWCNTs/CPE), which could act as an electro-catalytic tool for the analysis of N-acetylcysteine in this work. The BPOFc/BMPF6/NiO-SWCNTs/CPE with high electrical conductivity showed two completely separate signals with oxidation potentials of 432 and 970 mV for the first time that is sufficient for the determination of N-acetylcysteine in the presence of theophylline. The BPOFc/BMPF6/NiO-SWCNTs/CPE showed linear dynamic ranges of 0.02-300.0 μM and 1.0-350.0 μM with the detection limit of ~ 8.0 nM and 0.6 μM for the measurement of N-acetylcysteine and theophylline, respectively. In the second part, understanding the nature of interaction, quantum conductance modulation, electronic properties, charge density, and adsorption behavior of N-acetylcysteine on NiO-SWCNTs surface from first-principle studies through the use of theoretical investigation is vital for designing high-performance sensor materials. The N-acetylcysteine molecule was chemisorbed on the NiO-SWCNTs surface by suitable adsorption energies (- 1.102 to - 5.042 eV) and reasonable charge transfer between N-acetylcysteine and NiO-SWCNTs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-79872-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806823PMC
January 2021

Comparison of functional exercise capacity, quality of life and respiratory and peripheral muscle strength between patients with stable angina and healthy controls.

J Int Med Res 2020 Dec;48(12):300060520979211

Department Of Physiotherapy And Rehabilitation, Faculty of Health Sciences, Marmara University, Istanbul, Turkey.

Objective: We aimed to compare functional exercise capacity, respiratory and peripheral muscle strength, pulmonary function and quality of life between patients with stable angina and healthy controls.

Methods: We compared 33 patients with stable angina (55.21 ± 6.12 years old, Canada Class II-III, left ventricular ejection fraction: 61.92 ± 7.55) and 30 healthy controls (52.70 ± 4.22 years old). Functional capacity (6-minute walk test (6-MWT)), respiratory muscle strength (mouth pressure device), peripheral muscle strength (dynamometer), pulmonary function (spirometer) and quality of life (Short Form 36 (SF-36)) were evaluated.

Results: 6-MWT distance (499.20 ± 51.91 m versus 633.05 ± 57.62 m), maximal inspiratory pressure (85.42 ± 20.52 cmHO versus 110.44 ± 32.95 cmHO), maximal expiratory pressure (83.33 ± 19.05 cmHO versus 147.96 ± 54.80 cmHO) and peripheral muscle strength, pulmonary function and SF-36 sub-scores were lower in the angina group versus the healthy controls, respectively.

Conclusion: Impaired peripheral and respiratory muscle strength, reduction in exercise capacity and quality of life are obvious in patients with stable angina. Therefore, these parameters should be considered in stable angina physiotherapy programmes to improve impairments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0300060520979211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750767PMC
December 2020

Retraction Note: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line.

Sci Rep 2020 Nov 30;10(1):21236. Epub 2020 Nov 30.

Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000, Kütahya, Turkey.

Editor's Note: this Article has been retracted; the Retraction Note is available at https://doi.org/10.1038/s41598-020-77741-4 .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-77741-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705752PMC
November 2020

Tramadol sensing in non-invasive biological fluids using a voltammetric electronic tongue and an electrochemical sensor based on biomimetic recognition.

Int J Pharm 2021 Jan 28;593:120114. Epub 2020 Nov 28.

Biosensors and Nanotechnology Group, Department of Biology, Faculty of Sciences, Moulay Ismaïl University of Meknes, B.P. 11201, Zitoune, 50003 Meknes, Morocco. Electronic address:

Tramadol (TRA) is a weak opioid analgesic, prescribed to relieve mild to moderately severe pain. However, side effects of TRA overdoses, including vomiting, depression, tachycardia, convulsions, morbidity and mortality are often reported. In this study, an electrochemical sensor based on molecularly imprinted conductive polymer was firstly developed for the quantitative and non-invasive detection of TRA. Secondly, a voltammetric electronic tongue (VE-Tongue) combined with chemometric methods was used for the qualitative analysis. The MIP sensor was constructed by self-assembling a poly-aniline layer coated with silver nanoparticles (PANI-AgNPs) on a screen-printed gold electrode (Au-SPE). Then, 2-amino-thiophenol was polymerised in the presence of TRA. The electronic device exhibits, under optimal conditions, responses proportional to TRA concentrations (0.01-100 µg/mL) with detection and quantification limits of 9.42 µg/mL and 28.55 µg/mL, respectively. Moreover, its selectivity was proven by insignificant interferences of substances (paracetamol and citric acid). Spiked saliva and urine samples were used for the sensor practical application with a significant recovery above 90% and standard deviations below 4.5%. Besides, urine samples' analyses using VE-Tongue and pattern recognition methods show good discrimination, classification, and prediction results with scores above 95%. Correspondingly, both electro-analytical devices could be viable for monitoring drugs in biological matrices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2020.120114DOI Listing
January 2021

Fruit Extract Mediated Green Synthesis of Metallic Nanoparticles: A New Avenue in Pomology Applications.

Int J Mol Sci 2020 Nov 11;21(22). Epub 2020 Nov 11.

Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic.

Fruit extracts have natural bioactive molecules that are known to possess significant therapeutic potential. Traditionally, metallic nanoparticles were synthesized via chemical methods, in which the chemical act as the reducing agent. Later, these traditional metallic nanoparticles emerged as the biological risk, which prompted researchers to explore an eco-friendly approach. There are different eco-friendly methods employed for synthesizing these metallic nanoparticles via the usage of microbes and plants, primarily via fruit extract. These explorations have paved the way for using fruit extracts for developing nanoparticles, as they eliminate the usage of reducing and stabilizing agents. Metallic nanoparticles have gained significant attention, and are used for diverse biological applications. The present review discusses the potential activities of phytochemicals, and it intends to summarize the different metallic nanoparticles synthesized using fruit extracts and their associated pharmacological activities like anti-cancerous, antimicrobial, antioxidant and catalytic efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21228458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697565PMC
November 2020

Purification and characterization of thermostable α-amylase produced from Bacillus licheniformis So-B3 and its potential in hydrolyzing raw starch.

Life Sci 2021 Jan 24;264:118639. Epub 2020 Oct 24.

Sen Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100 Kütahya, Turkey.

Aims: This work was achieved to obtain the optimum culture conditions of the thermostable alpha-amylase produced by thermophilic Bacillus licheniformis SO-B3. Furthermore, the α-amylase was purified and then characterized, and also its kinetic parameters were determined.

Materials And Methods: A new thermotolerant bacteria called Bacillus licheniformis SO-B3 employed in this work was isolated from a sample of thermal spring mud in Şırnak (Meyremderesi). Several parameters such as the impact of temperature, time, and pH on enzyme production were examined. Thin-Layer Chromatography (TLC) was employed to analyze the end-products of soluble starch hydrolysis, and the utilization of purified α-amylase in the clarification of unripe apple juices was studied.

Key Findings: The highest enzyme production conditions were determined as 35 °C, 36th hour, and pH 7.0. Thermostable α-amylase was purified by 70% ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and dialysis, with a 51-purification fold and 30% yield recovery. The K and V values for this enzyme were 0.004 mM and 3.07 μmol min at 70 °C, respectively. The α-amylase's molecular weight was found as 74 kDa. In addition, α-amylase showed a good degradation rate for raw starch.

Significance: It was hypothesized that Bacillus licheniformis SO-B3 could be used as an α-amylase source. These findings displayed that purified enzyme could be utilized in fruit juice industries for clarification of apple juice and raw starch hydrolyzing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.118639DOI Listing
January 2021

Increased Rates of Coronary Artery Calcium Score in Patients with Non - Functioning Adrenal Incidentaloma.

Endocr Metab Immune Disord Drug Targets 2021 ;21(7):1319-1325

Department of Cardiology, Mustafa Kemal University, Hatay, Turkey.

Aim: We evaluated cardiovascular (CV) risk stratification for nonfunctioning adrenal incidentalomas (NFAIs) via the coronary-artery-calcium (CAC) score.

Materials And Methods: The participants were patients with NFAI (n = 55). They were compared to patients with chest pain, a low-intermediate Framingham-risk score, and a non-diagnostic treadmill- exercise test, which served as the control group (n = 49). Subsequently, the NFAI group was subdivided according to a CAC score of <100 Agatston units - mild coronary-artery calcification (n = 40) - and ≥100 Agatston units - moderate-to-severe calcification (n = 15).

Results: Similar rates of traditional risk factors were observed between the NFAI and control groups, and lower low-density lipoprotein cholesterol rates were observed in the NFAI group. The CAC score was significantly higher for the NFAI group than the control group. Glucose, potassium, adrenocorticotropic-hormone, and basal-cortisol levels were higher in those with a CAC score of ≥100. High-density-lipoprotein cholesterol estimated glomerular filtration rate and ejection fraction (EF) were higher in those with a CAC score of <100. Adenoma size and location were similar between the groups. Age, EF, and glucose were the most significant variables related to CAC score in patients with NFAI, at ≥100 Agatston units.

Discussion: Patients with a low-intermediate CV risk profile and NFAI have a higher risk of atherosclerosis when compared to patients with a low-intermediate CV risk profile, but no NFAI.

Conclusion: In patients with NFAI, CAC score evaluation may be used to predict increased atherosclerosis, especially in patients of an older age with higher glucose and decreased EF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1871530320666200910110337DOI Listing
January 2021

Retraction Note: Novel green synthesis and antioxidant, cytotoxicity, antimicrobial, antidiabetic, anticholinergics, and wound healing properties of cobalt nanoparticles containing Ziziphora clinopodioides Lam leaves extract.

Sci Rep 2020 09 7;10(1):14826. Epub 2020 Sep 7.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupinar University, Evliya Celebi Campus, 43100, Kutahya, Turkey.

Editor's Note: this Article has been retracted; the Retraction Note is available at https://www.nature.com/articles/s41598-020-71843-9.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-71843-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477173PMC
September 2020

Preparation and characterization of diatomite and hydroxyapatite reinforced porous polyurethane foam biocomposites.

Sci Rep 2020 08 6;10(1):13308. Epub 2020 Aug 6.

Department of Material Science and Engineering, İzmir Katip Çelebi University, İzmir, Turkey.

Porous three-dimensional (3D) polyurethane-based biocomposites were produced utilizing diatomite and hydroxyapatite as fillers. Diatomite and Hydroxyapatite (HA) were utilized to reinforce the morphological, chemical, mechanical, and thermal properties of polyurethane foam (PUF). Diatomite and Hydroxyapatite were added into polyurethane at variable percentages 0, 1, 2, and 5. The mechanical properties of PUF were analyzed by the compression test. According to the compression test results, the compressive strength of the polyurethane foam is highest in the reinforced foam at 1% by weight hydroxyapatite compared to other reinforced PUFs. Scanning electron microscopy (SEM) images presented structural differences on foam by adding fillers. Functional groups of PUF were defined by Fourier Transform Infrared Spectroscopy (FTIR) and the thermal behavior of PUF was studied with Thermogravimetric Analysis (TGA). The obtained results revealed that PUF/HA biocomposites indicated higher thermal degradation than PUF/Diatomite biocomposites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-70421-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413266PMC
August 2020

Synthesis of benzylidenemalononitrile by Knoevenagel condensation through monodisperse carbon nanotube-based NiCu nanohybrids.

Sci Rep 2020 Jul 29;10(1):12758. Epub 2020 Jul 29.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.

Monodisperse nickel/copper nanohybrids ([email protected]) based on multi-walled carbon nanotubes (MWCNT) were prepared for the Knoevenagel condensation of aryl and aliphatic aldehydes. The synthesis of these nanohybrids was carried out by the ultrasonic hydroxide assisted reduction method. [email protected] nanohybrids were characterized by analytical techniques such as X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and Raman spectroscopy. According to characterization results, [email protected] showed that these nanohybrids form highly uniform, crystalline, monodisperse, colloidally stable [email protected] nanohybrids were successfully synthesized. Thereafter, a model reaction was carried out to obtain benzylidenemalononitrile derivatives using [email protected] as a catalyst, and showed high catalytic performance under mild conditions over 10-180 min.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-69764-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391679PMC
July 2020

Metalo components exhibiting significant anticancer and antibacterial properties: a novel sandwich-type like polymeric structure.

Sci Rep 2020 07 27;10(1):12472. Epub 2020 Jul 27.

Biochemistry Department, Sen Research Group, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.

Four new dicyanoargentate(I)-based complexes 1-4 were synthesized from certain metal ions with a tetradentate ligand [N, N-bis (2-hydroxyethyl) -ethylenediamine; N-bishydeten] and determined by diverse procedures (elemental, thermal, FT-IR, ESI-MS for 1-3 and, magnetic susceptibility and EPR for 1, and 2) including crystal analysis of 4. The crystal method revealed that complex 4 has a sandwich-type like polymeric chemical structure with layers formed by [Cd(N-bishydeten)] cations and [Ag(CN)] anions. The complexes were further characterized by fluorescence and UV spectroscopy to determine their physicochemical features. The complexes displayed a DNA binding activity within the same range as found for cisplatin, in addition to their strong stability in the presence of the physiological buffer system. The complexes were also investigated for pharmacological properties like interaction with DNA/Bovine serum albumin, anticancer and antibacterial activities. Physicochemical studies of DNA with the complexes suggested that the interaction mode between them are possibly both intercalative and groove binding types. These spectroscopic measurements also show that there may be a binding tendency between BSA and the complexes via hydrogen or Van der Waals bonds. The viability tests demonstrated that all the complexes exhibited antibacterial (1-4) and anticancer effects (2-4) toward ten diverse bacterial strains and three tumor cells (HT-29 colon adenocarcinoma, HeLa cervical cancer, and C6 glioma), respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-69416-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385626PMC
July 2020

Novel green synthesis and antioxidant, cytotoxicity, antimicrobial, antidiabetic, anticholinergics, and wound healing properties of cobalt nanoparticles containing Ziziphora clinopodioides Lam leaves extract.

Sci Rep 2020 07 22;10(1):12195. Epub 2020 Jul 22.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupinar University, Evliya Celebi Campus, 43100, Kutahya, Turkey.

The aim of the experiment was a green synthesis of cobalt nanoparticles from the aqueous extract of Ziziphora clinopodioides Lam (CoNPs) and assessment of their cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing properties. The synthesized CoNPs were characterized using different techniques including UV-Vis., FT-IR spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray spectrometry (EDS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). According to the XRD analysis, 28.19 nm was measured for the crystal size of NPs. TEM and SEM images exhibited a uniform spherical morphology and average diameters of 29.08 nm for the biosynthesized nanoparticles. Agar diffusion tests were done to determine the antibacterial and antifungal characteristics. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) were specified by macro-broth dilution assay. CoNPs indicated higher antibacterial and antifungal effects than many standard antibiotics (p ≤ 0.01). Also, CoNPs prevented the growth of all bacteria at 2-4 mg/mL concentrations and removed them at 2-8 mg/mL concentrations (p ≤ 0.01). In the case of antifungal effects of CoNPs, they inhibited the growth of all fungi at 1-4 mg/mL concentrations and destroyed them at 2-16 mg/mL concentrations (p ≤ 0.01). The synthesized CoNPs had great cell viability dose-dependently and indicated this method was nontoxic. DPPH free radical scavenging test was done to assess the antioxidant potentials, which revealed similar antioxidant potentials for CoNPs and butylated hydroxytoluene. In vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% Co(NO) ointment, treatment with 0.2% Z. clinopodioides ointment, and treatment with 0.2% CoNPs ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at day 10. Use of CoNPs ointment in the treatment groups substantially raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and remarkably decreased (p ≤ 0.01) the wound area, total cells, neutrophil, and lymphocyte compared to other groups. In conclusion, CoNPs can be used as a medical supplement owing to their non-cytotoxic, antioxidant, antibacterial, antifungal, and cutaneous wound healing effects. Additionally, the novel nanoparticles (Co(NO) and CoNPs) were good inhibitors of the α-glycosidase, and cholinesterase enzymes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68951-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376013PMC
July 2020

Monodisperse palladium-cobalt alloy nanocatalyst supported on activated carbon (AC) as highly effective catalyst for the DMAB dehydrocoupling.

Sci Rep 2020 Jul 16;10(1):11755. Epub 2020 Jul 16.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Celebi Campus, 43100, Kutahya, Turkey.

In the study, activated carbon (AC) supported palladium/cobalt (Pd/Co) nanocatalyst was synthesized to achieve hydrogen release from dimethylamine boron (DMAB). Nanocatalyst were produced by the reduction of Pd and Co cations by the ultrasonic double reduction method. Analytical studies of the synthesized nanomaterials were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, X-ray diffraction, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), electron energy loss spectroscopy, ultraviolet-visible spectroscopy. In this research, nanomaterials exhibited high catalytic activity and reusability, and great performance at low temperatures and concentrations. For the dehydrogenation reaction of dimethylamine borane, TOF and Ea were calculated as 379.5 h and 75.86 kJ mol, respectively. The [email protected] nanocatalyst can be used as a promising catalyst for the hydrogen production reaction from DMAB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68773-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366684PMC
July 2020

Synthesis and characterization of trimeric phosphazene based ionic liquids with tetrafluoroborate anions and their thermal investigations.

Sci Rep 2020 Jul 16;10(1):11705. Epub 2020 Jul 16.

Department of Biochemistry, University of Dumlupınar, Kütahya, Turkey.

The quaternized compounds (PzIL1-9) reacted with sodium tetrafluoroborate (NaBF) to generate phosphazene based ionic liquids (PzILs), PzIL1a-9a. The newly synthesized ionic compounds (PzIL1a-9a) were verified using elemental CHN analyses and functional and spectroscopic (FTIR and H, C, P-NMR) analyses techniques. The thermal properties of PzIL1a-9a were investigated using thermogravimetric analysis (TGA). According to the initial decomposition temperature values calculated based on the TGA thermograms, PzIL7a (213 °C) was recognized to be more thermally stable than the other PzILs studied. PzIL1a-9a exhibited good solubility in the water and demonstrate a typical dielectric relaxation behavior, conductivity levels for both low and high-frequency regions. AC conductivity mechanisms and dielectric relaxation behavior of each sample are investigated by fabricating parallel plate capacitors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68709-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367273PMC
July 2020

A new nickel-based co-crystal complex electrocatalyst amplified by NiO dope Pt nanostructure hybrid; a highly sensitive approach for determination of cysteamine in the presence of serotonin.

Sci Rep 2020 07 16;10(1):11699. Epub 2020 Jul 16.

Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.

A highly sensitive electrocatalytic sensor was designed and fabricated by the incorporation of NiO dope Pt nanostructure hybrid (NiO-Pt-H) as conductive mediator, bis (1,10 phenanthroline) (1,10-phenanthroline-5,6-dione) nickel(II) hexafluorophosphate (B,1,10,P,1,10, PDNiPF6), and electrocatalyst into carbon paste electrode (CPE) matrix for the determination of cysteamine. The NiO-Pt-H was synthesized by one-pot synthesis strategy and characterized by XRD, elemental mapping analysis (MAP), and FESEM methods. The characterization data, which confirmed good purity and spherical shape with a diameter of ⁓ 30.64 nm for the synthesized NiO-Pt-H. NiO-Pt-H/B,1,10, P,1,10, PDNiPF6/CPE, showed an excellent catalytic activity and was used as a powerful tool for the determination of cysteamine in the presence of serotonin. The NiO-Pt-H/B,1,10, P,1,10, PDNiPF6/CPE was able to solve the overlap problem of the two drug signals and was used for the determination of cysteamine and serotonin in concentration ranges of 0.003-200 µM and 0.5-260 µM with detection limits of 0.5 nM and 0.1 µM, using square wave voltammetric method, respectively. The NiO-Pt-H/B,1,10,P,1,10,PDNiPF6/CPE showed a high-performance ability for the determination of cysteamine and serotonin in the drug and pharmaceutical serum samples with the recovery data of 98.1-103.06%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68663-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366926PMC
July 2020

Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line.

Sci Rep 2020 07 15;10(1):11631. Epub 2020 Jul 15.

Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000, Kütahya, Turkey.

In this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV-Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p ≤ 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they raised the cell viability and mitochondrial membrane potential in the high concentration of methadone-treated PC12 cells. The best result of neuroprotective properties was seen in the high dose of copper nanoparticles i.e., 4 µg. According to the above results, copper nanoparticles containing C. sativus leaf aqueous extract can be used in peripheral nervous system treatment as a neuroprotective promoter and central nervous system after approving in the clinical trial studies in humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68142-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363853PMC
July 2020
-->