Publications by authors named "Fatemeh Mohajerani"

5 Publications

  • Page 1 of 1

Evaluation of Neuroprtective Effects of L-Carnitine and Fat Emulsion in the CVA Patients: A Prospective, Randomized, Double Blind, Clinical Trial.

Iran J Pharm Res 2020 ;19(1):111-119

Department of Neurology, Bu Ali Sina General Hospital, Mazandaran University of Medical Sciences, Sari, Iran.

Cerebral infarction presents with neurological deficits caused by the death of neurons in a focal area of the brain. S100B is a biomarker that increases in brain damage. Neuroprotectives can reduce the brain sequels after neurological insult. The purpose of this study was to evaluate the neuroprotective effects of L-carnitine and Fat emulsion (Lipofundin) alone and in combination in patients with ischemic stroke. In a prospective, RCT, and double-blind study 100 patients with MCA ischemic cerebrovascular accident who were admitted in the first 24 h of injury entered the study. The patients were randomly assigned into four groups of L-carnitine, fat emulsion, L-carnitine plus fat emulsion and control. Fat emulsion 10%, 500 mL, was infused over 6 to 12 h and 1 gr of L-carnitine (10 mL of solution) was administered orally to patients in addition to common therapies, according to the American Heart Association and American Stroke Association (AHA/ASA) guidelines. The patients in the control group received only the usual treatment according to stroke guidelines. Blood samples before the intervention, then after 24 h, 48 h, and 7 days later were taken and immunoenzymatic colorimetric method was used for quantitative determination of S100B concentration in the patients' serum. In the within group analysis, all of our treatment interventions (except control group) have decreased S100B levels statistically significant ( < 0.05). Moreover, changes in observed levels of S100B before and after intervention were different between the groups and the observed differences were statistically significant ( = 0.01). In the GEE model, it was found that S100B levels in the L-carnitine plus fat emulsion group decreased more than the control group and this decline has been statistically significant [ = 0.02, 20.47 (CI 95%: 6.25-34.41)], but in comparison of L-carnitine and fat emulsion group with control group, did not reached statistical significance ( > 0.05). Based on the results obtained from this study, it seems that L-carnitine with fat emulsion could lead to neuroprotective effects with a significant reduction in the S100B biomarker.
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http://dx.doi.org/10.22037/ijpr.2020.1100952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462480PMC
January 2020

A Comparative Study of HOTAIR Expression in Breast Cancer Patient Tissues and Cell Lines.

Cell J 2020 Jul 14;22(2):178-184. Epub 2019 Oct 14.

Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina. Electronic

Objective: Recent data suggest that increased levels of the long non-coding RNA (lncRNA) are involved in the development of various types of malignancy, including breast cancer. The aim of present study was to investigate lncRNA expression profile in breast cancer (BC) patients and cell lines.

Materials And Methods: In this experimental study, expression level of lncRNA was evaluated in BC and normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reversetranscription polymerase chain reaction (qRT-PCR). lncRNA expression levels were estimated using 2 method. Further, receiver operating characteristic (ROC) curve analysis was done to evaluate the selected lncRNA diagnostic potential. The Cox's proportional hazards regression model was performed to evaluate the predictive value of this lncRNA level in BC patients.

Results: The results of present study demonstrated no significant difference in the expression of lncRNA in MCF7 and MDA-MB-231 cancer cell lines compared to MCF-10A as normal cell line (P>0.05). However, we observed a significantly increase in the expression of in BC patients compared to normal tissues (P<0.001). Significant associations were found between gene expression and tumour size and margin. We found 91.1% sensitivity and 95.7% specificity of circulating with an area under the ROC curve of 0.969. The Kaplan-Meier analysis indicated significant correlation between expression and overall survival.

Conclusion: This study demonstrated that expression of is increased in BC and might be associated with its progression. According to these findings, expression could be proposed as biomarkers for BC early diagnosis and prognosis.
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http://dx.doi.org/10.22074/cellj.2020.6543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874785PMC
July 2020

The implication of single-nucleotide polymorphisms in ovarian hyperstimulation syndrome.

Mol Reprod Dev 2019 08 22;86(8):964-971. Epub 2019 May 22.

Preventative Gynecology Research Center (PGRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Ovarian hyperstimulation syndrome (OHSS) is an undesirable complication in the course of ovarian stimulation. This kind of stimulation is aimed at acquiring a sufficient number of high-quality oocytes in in vitro fertilization (IVF). Whereas the predisposition to OHSS could be impacted by genetic polymorphisms in susceptible genes, the present study has been jointly conducted with an Iranian cohort to scrutinize its relevant implication. Genomic DNA was extracted from blood samples of patients with a normal ovarian response (NOR) or with OHSS. Samples were analyzed to detect polymorphisms MTHFR rs1801131, MTHFR rs1801133, AMHR2 rs2002555, LHCGR rs2293275, PGR rs10895068, and SERPINE1 rs1799889. Variations of MTHFR, AMHR2, LHCGR, and PGR genes were significantly associated with the developing OHSS. After correction for multiple analysis, this difference was not evident for PGR genotypes. The polymorphic alleles of MTHFR (rs1801131 C-allele and rs1801133 T-allele), AMHR2 (rs2002555 G-allele), and LHCGR (rs2293275 G-allele) were significantly more prevalent among patients with OHSS compared to those in the NOR group. In contrast, the minor allele of PGR single-nucleotide polymorphism (SNP) (rs10895068, A-allele) was more prominent among patients with a NOR than those with OHSS. No significant difference was observed in genotypes or alleles of SERPINE1 rs1799889. The observations indicated that the minor alleles of MTHFR, AMHR2, and LHCGR genes could be considered an independent risk factor in susceptibility to OHSS. Nevertheless, polymorphic allele in the PGR rs10895068 SNP contributes to preventing OHSS occurrence. Therefore, it can be argued that these genes have a significant impact on OHSS.
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http://dx.doi.org/10.1002/mrd.23171DOI Listing
August 2019

Novel Mutations in Gene in Families with Gelatinous Drop-like Corneal Dystrophy (GDLD).

Int J Mol Cell Med 2017 11;6(4):204-211. Epub 2017 Dec 11.

Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

In the current study, we conducted a mutation screening of tumor-associated calcium signal transducer 2 () gene in six consanguineous Iranian families with gelatinous drop-like corneal dystrophy (GDLD), in order to find the causative mutations. Detailed eye examination was performed by ophthalmologist to confirm GDLD in patients. To detect the possible mutations, direct Sanger sequencing was performed for the only exon of gene, and its boundary regions in all patients. In the patients with GDLD, the corneal surface showed lesions with different shapes from mild to severe forms depending on the progress of the disease. The patients showed grayish corneal deposits as a typical mulberry form, corneal dystrophy along with corneal lipid deposition, and vascularization. Targeted Sanger sequencing in gene revealed the causative mutations in this gene in all studied families. Our study expanded the mutational spectrum of which along with the related symptoms could help with the diagnosis, and management of the disease.
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http://dx.doi.org/10.22088/BUMS.6.4.204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004293PMC
December 2017

SNAP-25 gene variations and attention-deficit hyperactivity disorder in Iranian population.

Neurol Res 2016 Nov 15;38(11):959-964. Epub 2016 Sep 15.

f Department of Medical Genetics , School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran , Iran.

Objectives: Attention deficit hyperactivity disorder (ADHD) is a common psychiatric condition of childhood characterized by persistent symptoms of hyperactivity, inattention, and impulsivity. The objective of this study was to investigate the association of synaptosomal-associated protein of 25 kDa (SNAP-25) gene variants with ADHD.

Methods: A case-control study with a total of 150 children with ADHD (mean age 9.61; range 6-16; gender ratio 105m/45f) and 150 normal children (mean age 10.02; range 6-16; gender ratio 98m/52f) was conducted. Genomic DNA was extracted from peripheral blood of all samples and SNPs rs78428954 and rs3746544 located in SNAP-25 gene were genotyped.

Results: Our analysis indicated that there is no significant association between none of studied variants in SNAP-25 and ADHD.

Discussion: To our knowledge, it is the first report of SNAP-25 genotyping in Iranian patients with ADHD. Further investigations with larger populations are needed in order to clarify the exact role of SNAP-25 variations in susceptibility to ADHD.
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http://dx.doi.org/10.1080/01616412.2016.1232548DOI Listing
November 2016