Publications by authors named "Farzaneh Nikookhah"

5 Publications

  • Page 1 of 1

Multi-functionPlantDefensin,AntimicrobialandHeavyMetal Adsorbent Peptide.

Iran J Biotechnol 2019 Sep 1;17(3):e1562. Epub 2019 Sep 1.

Ecofibre Industries Operations and Ananda Hemp, Brisbane, Australia.

Background: Defensin peptide isolated from plants are often heterogeneous in length, sequence and structure, but they are mostly small, cationic and amphipathic. Plant defensins exhibit broad-spectrum antibacterial and antifungal activities against Gram-positive and Gram-negative bacteria, fungi and etc. Plant defensins also play an important role in innate immunity, such as heavy metal and some abiotic stresses tolerance.

Objectives: In this paper, broad-spectrum activities, antimicrobial and heavy metal absorption, of a recombinant plant defensin were studied.

Material And Methods: gene, a modified plant defensin gene, was cloned in pBISN1-IN (EU886197) plasmid, recombinant protein was produced by transient expression via Agroinfiltration method in common bean. The recombinant protein was tested for antibacterial activity against Gram-negative, Gram-positive bacteria and Fusarium sp. the effects of different treatments on heavy metal zinc absorption by this peptide were tested.

Results: We confirmed the antibacterial activities of this peptide against Gram-negative ( and ) and Gram-positive ( and ) bacteria, and antifungal activities of this peptide against Fusarium spp. ( and ). High metal absorption coefficient for this peptide was also observed.

Results: Out of six actinobacterial isolates, VITVAMB 1 possessed the most efficient RO-16 decolorization property. It decolorized 85.6% of RO-16 (250 mg L) within 24hrs. Isolate VITVAMB 1 was identified to be sp. Maximum dye decolorization occurred at pH 8, temperature 35oC, 3% salt concentration and a dye concentration of 50 mg L.

Conclusions: Results suggesting that modified defensin peptide facilitates a broader range of defense activities. dedefensins are an important part of the innate immune system in eukaryotes. These molecules have multidimensional properties that making them promising agents for therapeutic drugs.
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http://dx.doi.org/10.29252/ijb.1562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080970PMC
September 2019

In vitro efficacy for some local antimicrobial products against E. coli K12.

Pak J Biol Sci 2007 Dec;10(24):4570-2

Department of Pathobiology, School of Veterinary Science, Shahrekord University, P.O. Box 115, Shahrekord, Iran.

The aim of this study was to assess the in vitro activity of a number of antimicrobial agents (Sultrim, Oxytetracycline, enrofloxacin and florfenicol) produced by different Iranian manufacturers against E. coli K12. Comparison of the MICs of reference antimicrobials with Disk Diffusion (DD) results of different products made by Iranian manufacturers revealed that all products of tested antimicrobials seem to be in synergy with their reference MIC evaluation. Present data indicate that E. coli K 12 is resistant to Enrofloxacin (MIC value of 6.25 microg mL(-1)) by both MIC and DD evaluation.
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http://dx.doi.org/10.3923/pjbs.2007.4570.4572DOI Listing
December 2007

Isolation of Streptococci from milk samples of normal, acute and subclinical mastitis cows and determination of their antibiotic susceptibility patterns.

Pak J Biol Sci 2008 Jan;11(1):148-50

Department of Pathobiology, School of Veterinary Science, Shahrekord University, Shahrekord, Iran.

Streptococci are frequently isolated from bovine mastitis in dairy cows with only limited information available on the antimicrobial susceptibility of these organisms. A total of 42 Streptococci isolated from 148 milk samples of normal, sub acute and acute bovine mastitis cases. Overall, 35% of the strains tested were Streptococcus dysgalactiae, Streptococcus agalactiae 26%, Streptococcus uberis 18 and 4% were Enterococcus sp. Differences between the number of isolations in acute and sub acute groups were statistically significant, (p<0.5). The antimicrobial susceptibility for these organisms was determined for the following antimicrobial agents: cephalexine, penicillin, clindamycin, cloxaciline, gentamicin, streptomycin, amoxicillin, tetracycline, kanamycin, oxytetracycline, ampicillin, chloramphenicol and erythromycin. S. agalactiae, S. dysgalactiae, S. uberis and Enterococci demonstrated high level of resistance against streptomycin, penicillin and cloxaciline. Low level of sensitivity to other tested antimicrobials was demonstrated.
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http://dx.doi.org/10.3923/pjbs.2008.148.150DOI Listing
January 2008

Antimicrobial susceptibility of environmental bovine mastitis pathogens in west central Iran.

Pak J Biol Sci 2007 Sep;10(17):3014-6

Department of Pathobiology, School of Veterinary Science, Shahrekord University, P.O. Box 115, Shahrekord, Iran.

The purpose of this study was to describe antimicrobial susceptibility of environmental mastitis pathogens isolated from dairy herds of Chahar Mahal province in west central Iran. Out of the 620 milk samples collected from the four districts, 180 were CMT (California mastitis test) positive that cultured and yield, 7 (3.88%) coagulase negative Staphylococci (CNS), 15 (8.33%) Streptococci other than agalactiae and 17 (9.44%) E. coli. CNS resistance to penicillin was 14.28% but for streptomycin, Oxytetracycline and Colistin, were 28.57%. Non agalactiae Streptococci resistance to Oxytetracycline and Kanamycin were 20 and 13.33%, respectively. E. coli resistance to penicillin, oxytetracycline, streptomycin, erythromycin and Colistin were 88.23, 82.35, 76.47, 76.47 and 52.94%, respectively.
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http://dx.doi.org/10.3923/pjbs.2007.3014.3016DOI Listing
September 2007

Systemic dexamethasone and its effect on normal aerobic bacterial flora of cow.

Pak J Biol Sci 2007 Jun;10(12):2095-7

Department of Clinical Science, School of Veterinary Science, Shahrekord University, P.O. Box 115, Shahrekord, Iran.

This study was carried out on 17 Holestein, heifers, aged between 1 to 2 years for determining the normal aerobic bacterial flora and their changes after dexamethasone injection. Swab samples were taken from eye, ear, pharynx and vagina before and 5 days after twice dexamethasone treatment. Results indicated that Bacillus cereus and Corynebacterium pseudotuberculosis had higher frequency of isolations than the other bacterial flora in eye, ear and pharynx. Actinomyces pyogenes was isolated with considerable frequency from vagina. Klebsiella pneumoniae was also isolated from pharynx and its frequency was increased significantly after dexamethasone injection (p < 0.05).
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http://dx.doi.org/10.3923/pjbs.2007.2095.2097DOI Listing
June 2007