Publications by authors named "Farah Andleeb"

5 Publications

  • Page 1 of 1

Differentiation of Brain Tumor Microvasculature From Normal Vessels Using Optical Coherence Angiography.

Lasers Surg Med 2021 Jun 15. Epub 2021 Jun 15.

Department of Biomedical Engineering, The University of Texas Austin, Austin, Texas, 78712, USA.

Background And Objectives: Despite rapid advances and discoveries in medical imaging, monitoring therapeutic efficacy for malignant gliomas and monitoring tumor vasculature remains problematic. The purpose of this study is to utilize optical coherence angiography for vasculature characterization inside and surrounding brain tumors in a murine xenograft brain tumor model. Features included in our analysis include fractional blood volume, vessel tortuosity, diameter, orientation, and directionality.

Study Design/materials And Methods: In this study, five tumorous mice models at 4 weeks of age were imaged. Human glioblastoma cells were injected into the brain and allowed to grow for 4 weeks and then imaged using optical coherence tomography.

Results: Results suggest that blood vessels outside the tumor contain a greater fractional blood volume as compared with vessels inside the tumor. Vessels inside the tumor are more tortuous as compared with those outside the tumor. Results indicate that vessels near the tumor margin are directed inward towards the tumor while normal vessels show a more random orientation.

Conclusion: Quantification of vascular microenvironments in brain gliomas can provide functional vascular parameters to aid various diagnostic and therapeutic studies. © 2021 Wiley Periodicals LLC.
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June 2021

Multimodal imaging of skin lesions by using methylene blue as cancer biomarker.

Microsc Res Tech 2020 Dec 14;83(12):1594-1603. Epub 2020 Aug 14.

Biophotonics Imaging Techniques Laboratory, Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

This study aimed the optical imaging of malignant and normal skin tissues with multimodal wide-field fluorescence polarization imaging (WF) technique, by using methylene blue as fluorescence dye. We present optical imaging of skin tissues by different techniques, including reflectance, fluorescence, and polarization imaging for early detection of skin cancer. We collected the reflectance confocal images at 390 and 500 nm. For wide-field fluorescence images, specimens were stimulated at 640 nm and images were collected between 670 and 710 nm. The correlation of the regarded optical modalities with histopathology (H&E), their potentials, capabilities, and limitations to detect skin lesions are discussed. The advantages of multimodal imaging of skin tissues are analyzed to divulge possibilities for precise tumor boundary detection and their classification for malignant and nonmalignant skin tissues. Prior to imaging, the cells were stained in aqueous MB (a dye approved by FDA).
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December 2020

Hemoglobin structure at higher levels of hemoglobin A1C in type 2 diabetes and associated complications.

Chin Med J (Engl) 2020 May;133(10):1138-1143

Biophotoics Research Group, Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.

Background: Fourier transform infrared (FTIR) spectroscopy technique has not been used as a diagnostic tool for diabetes in clinical practice. This study was linked to structural changes in hemoglobin (Hb) in type 2 diabetes patients at higher levels of HbA1C using FTIR spectroscopy.

Methods: Fifty-three diabetic patients from the Bahawal Victoria Hospital, Bahawalpur, Pakistan were categorized as group A (6% < HbA1C < 7%; n = 25) and group B (HbA1C ≥9%; n = 28). Another group (group N) of twenty blood samples was taken from healthy people from the Islamia University Bahawalpur, Pakistan. Data from all groups were collected from January 1, 2018 to March 31, 2019. The structure of Hb was studied by FTIR spectroscopy and impact of glucose on the fine structure of HbA1C was estimated.

Results: Hb secondary structure erythrocyte parameters were altered by changing glucose concentrations. From FTIR spectra of all three groups it was found that Hb structure was slightly altered in group A, but significantly changed in group B (P < 0.05). There was an increase in β-sheet structure and a reduction in α-helix structure at elevated levels of HbA1C (group B) in type 2 diabetes.

Conclusion: We suggest that higher level of glycation reflected by increased HbA1C might be a contributing factor to structural changes in Hb in type 2 diabetic patients. FTIR spectroscopy can be a novel technique to find pathogenesis in type 2 diabetes.
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May 2020

Hematological complications under hyper conditions of glucose and distilled water.

Pak J Pharm Sci 2020 Mar;33(2):589-595

Bio-Photonics Research Laboratory Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

This work presents a pilot method of hematological diagnosis about changes in: shape, size and rouleaux formation, cell count of leucocytes and platelet cells in the presence of different glucose [CHO] and water [H2O] concentrations. The 2D microscopic images after addition of ten different glucose concentrations to normal blood (0 mM- 450 mM) revealed the lyses (disintegration) of white blood cells (WBCs). This work provides a baseline to diagnose blood disorders and complications at labs and clinical environment.
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March 2020

Attenuated total reflectance spectroscopy to diagnose skin cancer and to distinguish different metastatic potential of melanoma cell.

Cancer Biomark 2018 ;23(3):373-380

Biophotoics Research Group, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

Early diagnosing of skin cancer and investigation of metastatic potential of cancer cells are very important to treat it appropriately. Infrared spectroscopy of biological tissues is an emerging technique which gives the spectral differences between healthy and diseased cells. In this work, we have demonstrated that attenuated total reflectance Fourier transform (ATR-FTIR) spectroscopy can be used in diagnostic of skin cancer and in differentiating metastatic potential of cancer cells. Using IR spectroscopy, we can identify various types of cancer such as basal cell carcinoma, malignant melanoma, nevus and metastatic potential by alternations in hydration level and molecular changes. We examined biopsy of different types of cancer cells to diagnose skin cancer at early stages by using FTIR spectroscopy. To differentiate metastases we examined two human melanoma cells of same patient but at different metastatic potential and two murine melanoma cells with common genetic background but different metastatic potential. Our findings revealed that melanoma changes the permeability of cell membrane and higher metastatic potential is related to the hydration level of cell membrane. Thus, ATR-FTIR spectroscopy is a potential technique to help in early diagnosing of skin cancer and to differentiate different metastatic potentials.
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March 2019