Publications by authors named "Fanjing Meng"

16 Publications

  • Page 1 of 1

Lysosomal iron recycling in mouse macrophages is dependent upon both LcytB and Steap3 reductases.

Blood Adv 2022 Mar;6(6):1692-1707

Iron that is stored in macrophages as ferritin can be made bioavailable by degrading ferritin in the lysosome and releasing iron back into the cytosol. Iron stored in ferritin is found as Fe3+ and must be reduced to Fe2+ before it can be exported from the lysosome. Here we report that the lysosomal reductase Cyb561a3 (LcytB) and the endosomal reductase six-transmembrane epithelial antigen of prostate 3 (Steap3) act as lysosomal ferrireductases in the mouse macrophage cell line RAW264.7 converting Fe3+ to Fe2+ for iron recycling. We determined that when lysosomes were loaded with horse cationic ferritin, reductions or loss of LcytB or Steap3 using CRISPR/Cas9-mediated knockout technology resulted in decreased lysosomal iron export. Loss of both reductases was additive in decreasing lysosomal iron export. Decreased reductase activity resulted in increased transcripts for iron acquisition proteins DMT1 and transferrin receptor 1 (Tfrc1) suggesting that cells were iron limited. We show that transcript expression of LcytB and Steap3 is decreased in macrophages exposed to Escherichia coli pathogen UTI89, which supports a role for these reductases in regulating iron availability for pathogens. We further show that loss of LcytB and Steap3 in macrophages infected with UTI89 led to increased proliferation of intracellular UTI89 suggesting that the endolysosomal system is retaining Fe3+ that can be used for proliferation of intravesicular pathogens. Together, our findings reveal an important role for both LcytB and Steap3 in macrophage iron recycling and suggest that limiting iron recycling by decreasing expression of endolysosomal reductases is an innate immune response to protect against pathogen proliferation and sepsis.
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http://dx.doi.org/10.1182/bloodadvances.2021005609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941456PMC
March 2022

Prognostic signatures associated with high infiltration of Tregs in bone metastatic prostate cancer.

Aging (Albany NY) 2021 07 6;13(13):17442-17461. Epub 2021 Jul 6.

Key Laboratory of Tumor Immunity, Department of Immunology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.

Metastatic cancer especially bone metastasis (BM) is the lethal end-stage of castration-resistant prostate cancer (CRPC). To understand the possible molecular mechanisms underlying the development of the distant metastasis is of potential clinical value. We sought to identify differentially expressed genes between patient-matched primary and bone metastatic CRPC tumors. Functional enrichment, protein-protein interaction networks, and survival analysis of DEGs were performed. DEGs with a prognostic value considered as candidate genes were evaluated, followed by genetic analysis of tumor infiltrating immune cells based on Wilcoxon test and immunofluorescence identification. Expression profiles analysis showed that 381 overlapping genes were screened as differentially expressed genes (DEGs), of which 16 DEGs were randomly selected to be validated and revealed that most of these genes showed a transcriptional profile similar to that seen in the datasets (Pearson's r = 0.76). Six core genes were found to be involved in regulation of extracellular matrix receptor interaction and chemotactic activity, and four of them were significantly correlated with the survival of PCa patients with bone metastases. Immune infiltration analysis showed that the expressions levels of COL3A1, RAC1, FN1, and SDC2 in CD4+T cells were significantly higher than those in tumor cells, especially regulatory T cell infiltration was significantly increased in BM tumors. We analyzed gene expression signatures specifically associated with the development of bone metastases of CRPC patients. Characterization of genes associated with BM of mCRPC is critical for identification of predictive biomarkers and potential therapeutic targets.
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http://dx.doi.org/10.18632/aging.203234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312432PMC
July 2021

Association of vitamin E on the risk of ovarian cancer: a meta-analysis.

Biosci Rep 2019 12;39(12)

Department of General Medical Department, Longhua District Central Hospital, Shenzhen, 518110, Guangdong Province, China.

Many researches were conducted to assess the association of vitamin E intake on the risk of ovarian cancer, with conflict results. The current meta-analysis of published observational studies aimed to investigate the effect of vitamin E intake on ovarian cancer risk. The summary relative risks (RRs) with corresponding 95% confidence intervals (CIs) were calculated to measure the effectiveness of vitamin E intake on ovarian cancer risk using a random-effects model. As a result, 14 studies including 4597 patients were identified. Eleven studies reported about total vitamin E intake, eight studies about vitamin E intake from food only and five studies about vitamin E intake from supplement only on the risk of ovarian cancer. Overall, the summary RRs on ovarian cancer risk was 0.95 (95%CIs = 0.78-1.16) in total vitamin E intake, 0.99 (95%CIs = 0.77-1.27) in vitamin E intake from food only and 0.82 (95%CIs = 0.54-1.25) in vitamin E intake from supplement only. Results in subgroup analyses by study design and geographic location were consistent with overall result. In conclusions, the findings of this meta-analysis suggested that high intake of vitamin E from food or vitamin E supplement had no significant effect on the risk of ovarian cancer.
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http://dx.doi.org/10.1042/BSR20193311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928519PMC
December 2019

Astragaloside IV Exerts a Myocardial Protective Effect against Cardiac Hypertrophy in Rats, Partially via Activating the Nrf2/HO-1 Signaling Pathway.

Oxid Med Cell Longev 2019 11;2019:4625912. Epub 2019 Jun 11.

Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining 272029, China.

Previous evidence suggested that astragaloside IV (ASIV) had a cardioprotective effect, but the potential mechanisms were undetermined. This study is aimed at validating the prevention of cardiac hypertrophy in chronic heart failure (CHF) rats and hypertrophy in H9c2 cardiomyocytes by ASIV and at exploring the potential mechanism involved. CHF rat models of abdominal aortic constriction (AAC) were used with the aim of determining the protective effect of ASIV in cardiac hypertrophy in the rats. We proved that ASIV could attenuate cardiac hypertrophy by improving left ventricular function and structure and showed that the expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream gene heme oxygenase-1 (HO-1) increased in the high-dose ASIV intervention group. To further investigate the specific mechanism of ASIV, we hypothesized that ASIV might prevent cardiac hypertrophy via activating the Nrf2/HO-1 signaling pathway. We established a cardiomyocyte hypertrophy model induced by angiotensin II (Ang II), which was then transfected with Nrf2 shRNA, to knock down the expression of the Nrf2 gene. We found that the protective effect of ASIV against Ang II-induced cardiomyocyte hypertrophy was abolished in the Nrf2 shRNA transfection group, ultimately aggravating cardiomyocyte hypertrophy induced by Ang II, and it is possible that oxidative stress may be involved in this process. Our results demonstrated that ASIV improved cardiac function and inhibited cardiac hypertrophy by upregulating Nrf2, and this effect was partially achieved by stimulating the Nrf2/HO-1 signaling pathway, suggesting that ASIV could have therapeutic potential for the treatment of cardiac hypertrophy and CHF.
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http://dx.doi.org/10.1155/2019/4625912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594267PMC
January 2020

Ferritin in serum and urine: A pilot study.

Blood Cells Mol Dis 2019 05 8;76:59-62. Epub 2019 Feb 8.

Division of Hematology/Oncology, University of Utah, Salt Lake City, UT, USA.

Serum ferritin reflects total body iron stores, thus a low serum ferritin is used as a parameter of iron deficiency. In healthy adults in Japan, urine ferritin levels were about 5% of serum ferritin levels, with a correlation coefficient of 0.79. It is not known whether a low urine ferritin could serve as a non-invasive screen for iron deficiency. If so, this might be useful for neonates and young children, avoiding phlebotomy to screen for iron deficiency. However, for urinary ferritin screening to be feasible, ferritin must be measurable in the urine and correlate with serum ferritin. Testing should also clarify whether the iron content of ferritin in serum and urine are similar. In this pilot feasibility study we measured ferritin in paired serum and urine samples of healthy adult males, healthy term neonates, growing preterm neonates, and children who had very high serum ferritin levels from liver disorders or iron overload. We detected ferritin in every urine sample, and found a correlation with paired serum ferritin (Spearman correlation coefficient 0.78 of log-transformed values). These findings suggest merit in further studying urinary ferritin in select populations, as a potential non-invasive screen to assess iron stores.
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http://dx.doi.org/10.1016/j.bcmd.2019.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420832PMC
May 2019

Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea.

Mediators Inflamm 2017 29;2017:4265898. Epub 2017 Nov 29.

Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, China.

Residues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of , , and M3 are selected to reuse herb residue of Jianweixiaoshi tablets (JT), and an antibiotic-associated diarrhea (AAD) mouse model was established to evaluate the therapeutic effects of the herb residue fermentation supernatant. Our results indicated that the fermentation supernatant had scavenged 77.8% of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 78% of O, 36.7% of OH, 39% of Fe chelation, and 716 mg/L reducing power. The inhibition zones for , , , , , 301, and were 17, 14, 19, 18, 20, 19, and 20 mm, respectively. The in vivo results indicated that the fermentation supernatant resulted in a high diarrhea inhibition rate (56%, < 0.05), greatly enhanced the disruption of bacterial diversity caused by antibiotics, and restored the dominant position of in the treatment and recovery stages. Therefore, the combination of the herb residue and probiotics suggests a potential to explore conversion of these materials for the possible development of therapies for AAD.
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http://dx.doi.org/10.1155/2017/4265898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727800PMC
August 2018

Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus.

Microb Pathog 2018 Feb 3;115:299-303. Epub 2018 Jan 3.

School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China. Electronic address:

Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 10 CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P < .01). Our data indicated that Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.
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http://dx.doi.org/10.1016/j.micpath.2017.12.080DOI Listing
February 2018

Therapeutic effect of herb residue fermentation supernatant on spleen‑deficient mice.

Mol Med Rep 2018 Feb 24;17(2):2764-2770. Epub 2017 Nov 24.

Jiangxi‑OAI Joint Research Institute, Nanchang University, Nanchang, Jiangxi 330047, P.R. China.

To minimize the waste of active ingredients present in herb residues, in the present study, probiotics of Bacillus subtilis, Aspergillus oryzae and Lactobacillus plantarum M3 were selected to reuse herb residues from Jianweixiaoshi tablets, and the therapeutic effects of the herb residue fermentation supernatant were evaluated using a spleen‑deficient mouse model. The results of the present study indicated that the fermentation supernatant may effectively improve the immunity of mice, as measured by body weight, spleen and thymus index, and inflammatory cytokines, including interleukin (IL)‑2, IL‑4 and interferon‑γ. The viable cell count and denaturing gradient gel electrophoresis results indicated that the fermentation supernatant markedly enhanced bacterial diversity and the number of lactobacilli in mouse intestines. Therefore, the combination of the Jianweixiaoshi herb residue and probiotics provided a novel method to reuse herb residues and may in the future contribute to the treatment of spleen deficiency.
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http://dx.doi.org/10.3892/mmr.2017.8150DOI Listing
February 2018

Evaluation of the accuracy and sensitivity of high‑throughput sequencing technology using known microbiota.

Mol Med Rep 2018 Jan 20;17(1):408-413. Epub 2017 Oct 20.

Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China.

Next generation sequencing provides an excellent platform to explore microbiota in any given environment, and little work is required to evaluate the accuracy and sensitivity of high‑throughput sequencing technology. In the present study, a known microbiota containing Escherichia coli, Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium bifidum, Bacillus subtilis, Enterococcus faecalis and Salmonella typhimurium was used to evaluate the high‑throughput sequencing technology. The results suggested that there were 122.7 operational taxonomic units (OTUs) in all groups, which is 17.5‑fold (the whole OTU number/the actual bacterial number) greater compared with the actual microbial number in each group, and the Venn method indicated that only 46.38% (64/138), 58.70% (81/138), 86.13% (118/137), 83.57% (117/140) and 89.29% (125/140) of the common OTUs were identified in groups A, B, C, D and E, of which the majority of OTUs did not belong to known bacteria. In addition, the DNA extraction and amplification efficiency failed to identify bacteria at the phylum, class, order, family, genus and species levels, which may further increase false information of microbial analysis. In conclusion, the present study provided basic datato investigate the potential drawbacks of high‑throughput sequencing technology, which will help researchers to avoid exaggerating the bacterial number when this technology is applied to study microbiota in particular environments.
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http://dx.doi.org/10.3892/mmr.2017.7849DOI Listing
January 2018

Effective dispersion of multi-walled carbon nanotubes in aqueous solution using an ionic-gemini dispersant.

J Colloid Interface Sci 2018 Feb 31;512:750-757. Epub 2017 Oct 31.

College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, China.

A promising ionic-gemini molecule, 4, 4'-di (n-tetradecyl) diphenylmethane disulfate salt (DSDM), is reported for effective dispersion of multi-walled carbon nanotubes (MWCNTs) in aqueous medium in the present investigation. The dispersibility and stability of the DSDM-modified MWCNTs were characterized by UV-vis spectrophotometer, Raman spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy and zeta potential measurements. The hydrophobic interaction between alky chains and carbon nanotubes as well as the π-π-stacking interaction between benzene rings and carboatomic rings in MWCNTs enables a successful modification of DSDM onto the MWCNT surface. The dispersed MWCNTs individually existed in dispersion with no structural damage, indicating a much better performance than the MWCNTs dispersed by the sodium dodecylbenzene sulfonate (SDBS), a frequently reported single-chain ionic dispersant. Surface potential measurements showed that the DSDM-modified MWCNTs were negatively charged, giving rise to electrostatic repulsion between the MWCNTs in aqueous solution. A better MWCNT dispersion effect was observed with the increase in MWCNT surface potential, and the dispersion with high MWCNT surface potential presents high dispersion stability with no agglomeration appeared for more than 5 months. The magnesium (Mg) matrix composite fabricated based on the DSDM-dispersed MWCNTs demonstrated excellent mechanical properties compared to pure Mg. Our research may provide an alternative way to improve the mechanical properties of composites.
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http://dx.doi.org/10.1016/j.jcis.2017.10.109DOI Listing
February 2018

High‑throughput sequencing analyses of oral microbial diversity in healthy people and patients with dental caries and periodontal disease.

Mol Med Rep 2017 Jul 17;16(1):127-132. Epub 2017 May 17.

Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China.

Recurrence of oral diseases caused by antibiotics has brought about an urgent requirement to explore the oral microbial diversity in the human oral cavity. In the present study, the high‑throughput sequencing method was adopted to compare the microbial diversity of healthy people and oral patients and sequence analysis was performed by UPARSE software package. The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64, 53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria, respectively, were identified in healthy people. Moreover, the reduction of Firmicutes and the increase of Proteobacteria in the children group, and the increase of Firmicutes and the reduction of Proteobacteria in the youth and adult groups, indicated that the age bracket and oral disease had largely influenced the tooth development and microbial development in the oral cavity. In addition, the traditional 'pathogenic bacteria' of Firmicutes, Proteobacteria and Bacteroidetes (accounted for >95% of the total sequencing number in each group) indicated that the 'harmful' bacteria may exert beneficial effects on oral health. Therefore, the data will provide certain clues for curing some oral diseases by the strategy of adjusting the disturbed microbial compositions in oral disease to healthy level.
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http://dx.doi.org/10.3892/mmr.2017.6593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482155PMC
July 2017

Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection.

J Infect Public Health 2017 Nov - Dec;10(6):749-754. Epub 2017 Feb 8.

Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated with Shandong University, PR China.

Environmental pollution caused by herb residues and the huge waste of medicinal ingredients contained in herb residues hinder the development of traditional Chinese medicine enterprises. To solve this problem, several probiotics were tested, and Lactobacillus plantarum (HM218749) was finally selected for the reuse of herb residues of Jianweixiaoshi tablets. A mouse model of Helicobacter pylori infection was developed to evaluate the anti-H. pylori infection activity of the herb residue fermentation supernatant using a urease activity test, histological imaging, an enzyme-linked immunosorbent assay (ELISA) and denaturing gel gradient electrophoresis (DGGE). The results demonstrated that the herb residue fermentation supernatant successfully inhibited urease activity, slowed cell infiltration in the gastric area and significantly reduced the production of interleukin-6 (IL-6), IL-8 and TNF-α in the treatment group (p<0.01). In addition, the DGGE results indicated that the herb residue fermentation supernatant was beneficial for the recovery of the disturbed microbiota in the infected model to the normal condition, in which L. gasseri (GU417842.1) and L. johnsonii (HQ828141.1) were dominant in all groups. Therefore, the probiotics exhibited strong potential for the development of herb residues in this study, and the products showed strong potential in curing H. pylori infections.
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http://dx.doi.org/10.1016/j.jiph.2016.11.013DOI Listing
June 2018

Evaluation of the Microbial Diversity in Amyotrophic Lateral Sclerosis Using High-Throughput Sequencing.

Front Microbiol 2016 20;7:1479. Epub 2016 Sep 20.

Institute of Translational Medicine, Nanchang University Nanchang, China.

More and more evidences indicate that diseases of the central nervous system have been seriously affected by fecal microbes. However, little work is done to explore interaction between amyotrophic lateral sclerosis (ALS) and fecal microbes. In the present study, high-throughput sequencing method was used to compare the intestinal microbial diversity of healthy people and ALS patients. The principal coordinate analysis, Venn and unweighted pair-group method using arithmetic averages (UPGMA) showed an obvious microbial changes between healthy people (group H) and ALS patients (group A), and the average ratios of , , , , , and at genus level between ALS patients and healthy people were 0.78, 2.18, 3.41, 0.35, 0.79, and 13.07. Furthermore, the decreased Firmicutes/Bacteroidetes ratio at phylum level using LEfSE (LDA > 4.0), together with the significant increased genus (harmful microorganisms) and significant reduced genus , , (beneficial microorganisms) in ALS patients, indicated that the imbalance in intestinal microflora constitution had a strong association with the pathogenesis of ALS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028383PMC
http://dx.doi.org/10.3389/fmicb.2016.01479DOI Listing
September 2016

Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1.

Mediators Inflamm 2016 17;2016:2945650. Epub 2016 Jul 17.

Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, China.

Little work is done to develop Aloe vera (AV) using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacillus plantarum HM218749.1 had very strong scavenging capacities of the DPPH (86%), O2 (•-) (85%), (•)OH (76%), and Fe(2+) chelation (82%) and reducing powers (242.5 mg/L), and the inhibition zones for Salmonella typhimurium, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Listeria monocytogenes, S. dysenteriae 301, Staphylococcus aureus Cowan1, and Propionibacterium acnes were 16, 15, 19, 20, 21, 20, and 27 mm. Moreover, the low concentration of Aloe fermentation supernatant had significantly reduced the production of IL-1β, TNF-α, and IL-6 in both mRNA and protein levels (P < 0.01). Therefore, the Aloe fermentation supernatant can be used as functional beverage or cosmetic ingredients to guard human intestinal health, delaying senescence, and prevent chronic diseases.
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http://dx.doi.org/10.1155/2016/2945650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967468PMC
June 2017

RNA interference-mediated silencing of NANOG leads to reduced proliferation and self-renewal, cell cycle arrest and apoptosis in T-cell acute lymphoblastic leukemia cells via the p53 signaling pathway.

Leuk Res 2013 Sep 14;37(9):1170-7. Epub 2013 May 14.

Department of Hematology, Nanjing Medical University, Nanjing, China.

NANOG is critical for maintaining the self-renewal and proliferative properties of embryonic stem cells. Here we found that cultured T-cell acute lymphoblastic leukemia (T-ALL) cells, as well as human primary T-ALL cells, express a functional variant of NANOG. NANOG mRNA is derived predominantly from a retrogene locus termed NANOGP8. Furthermore, we showed that RNA interference-mediated NANOG knockdown inhibited cell proliferation, reduced self-renewal, promoted apoptosis and arrested the cell cycle through a p53-mediated pathway in leukemic cells. These findings demonstrate the oncogenic potential of this pluripotent gene in human T-ALL cells.
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http://dx.doi.org/10.1016/j.leukres.2013.04.021DOI Listing
September 2013

Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad.

BMC Genomics 2011 Oct 1;12:481. Epub 2011 Oct 1.

Noncoding RNA laboratory, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Background: Ascaris suum (large roundworm of pigs) is a parasitic nematode that causes substantial losses to the meat industry. This nematode is suitable for biochemical studies because, unlike C. elegans, homogeneous tissue samples can be obtained by dissection. It has large sperm, produced in great numbers that permit biochemical studies of sperm motility. Widespread study of A. suum would be facilitated by more comprehensive genome resources and, to this end, we have produced a gonad transcriptome of A. suum.

Results: Two 454 pyrosequencing runs generated 572,982 and 588,651 reads for germline (TES) and somatic (VAS) tissues of the A. suum gonad, respectively. 86% of the high-quality (HQ) reads were assembled into 9,955 contigs and 69,791 HQ reads remained as singletons. 2.4 million bp of unique sequences were obtained with a coverage that reached 16.1-fold. 4,877 contigs and 14,339 singletons were annotated according to the C. elegans protein and the Kyoto Encyclopedia of Genes and Genomes (KEGG) protein databases. Comparison of TES and VAS transcriptomes demonstrated that genes participating in DNA replication, RNA transcription and ubiquitin-proteasome pathways are expressed at significantly higher levels in TES tissues than in VAS tissues. Comparison of the A. suum TES transcriptome with the C. elegans microarray dataset identified 165 A. suum germline-enriched genes (83% are spermatogenesis-enriched). Many of these genes encode serine/threonine kinases and phosphatases (KPs) as well as tyrosine KPs. Immunoblot analysis further suggested a critical role of phosphorylation in both testis development and spermatogenesis. A total of 2,681 A. suum genes were identified to have associated RNAi phenotypes in C. elegans, the majority of which display embryonic lethality, slow growth, larval arrest or sterility.

Conclusions: Using deep sequencing technology, this study has produced a gonad transcriptome of A. suum. By comparison with C. elegans datasets, we identified sets of genes associated with spermatogenesis and gonad development in A. suum. The newly identified genes encoding KPs may help determine signaling pathways that operate during spermatogenesis. A large portion of A. suum gonadal genes have related RNAi phenotypes in C. elegans and, thus, might be RNAi targets for parasite control.
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http://dx.doi.org/10.1186/1471-2164-12-481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203103PMC
October 2011
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