Publications by authors named "Fang Yang"

1,485 Publications

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Modeling of UV diffused-LOS communication channel incorporating obstacle and its applicability analysis.

Opt Lett 2021 Sep;46(18):4578-4581

Existing studies of the ultraviolet (UV) channel mainly focus on non-line-of-sight (NLOS) scenarios, while line-of-sight (LOS) scenarios are generally neglected since obstacles sometimes block them. To fill this gap, a UV diffused-LOS channel model in the presence of an obstacle is proposed for the first time, to the best of our knowledge. For easy interpretation, the whole derivation is combined with four typical positions of the obstacle. Moreover, the applicability of the proposed model is investigated via comparison with the classical Monte Carlo simulation model, where the transceiver elevation angle and azimuth angle are always kept optimal. Calculation and simulation results show that when the obstacle thickness is relatively small, the channel attenuation of the UV diffused-LOS system is lower than that of the UV NLOS system with the variation of obstacle width, height, and distance between transceiver and obstacle. In addition, an expression of the impulse response time of the diffused-LOS channel with the obstacle is also derived for tractable analysis.
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http://dx.doi.org/10.1364/OL.438723DOI Listing
September 2021

Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments.

Front Pharmacol 2021 24;12:729414. Epub 2021 Aug 24.

Comparative Medicine Department of Researching and Teaching, Dalian Medical University, Dalian, China.

Naringin has been shown to exert protective effects in an animal model of ulcerative colitis, but detailed mechanisms remain unclear. This study aimed to investigate function and signaling mechanisms underlying naringin-induced therapeutic effects on colitis. Two mouse models were established to mimic human Inflammatory bowel disease (IBD) by treating drinking water with dextran sodium sulphate or intra-colonic administration of 2, 4, 6-trinitrobenzene sulfonic acid. Transcriptomics combined with functional experiments were used to investigate underlying mechanisms. Colitis symptoms, including weight loss and high disease activity index were significantly reversed by naringin. The inflammatory response, oxidative reactions, and epithelial cell apoptosis that occur with colitis were also alleviated by naringin. After naringin treatment, transcriptomics results identified 753 differentially expressed mRNAs that were enriched in signaling pathways, including the neuroactive ligand-receptor interaction, calcium signaling, and peroxisome proliferator-activated receptor (PPAR) signaling. The naringin-induced alleviation of colitis was significantly inhibited by the PPAR-γ inhibitor BADGE. In IEC-6 and RAW264.7 cells incubated with lipopolysaccharide (LPS), NF-κB-p65, a downstream protein of PPAR-γ, was significantly increased. Naringin suppressed LPS-induced high expression of NF-κB-p65, which was inhibited by small interfering RNA targeting PPAR-γ. Our study clarifies detailed mechanisms underlying naringin-induced therapeutic effects on mice colitis, and PPAR-γ was found to be the main target of naringin by functional experiments both and . Our study supplies new scientific information for the use of naringin in colitis treatment.
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http://dx.doi.org/10.3389/fphar.2021.729414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421552PMC
August 2021

Genome-Wide Identification and Analysis of the Metallothionein Genes in Genus.

Int J Mol Sci 2021 Sep 6;22(17). Epub 2021 Sep 6.

State Key Laboratory of Hybrid Rice, Hongshan Laboratory of Hubei Province, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan 430072, China.

Metallothionein (MT) proteins are low molecular mass, cysteine-rich, and metal-binding proteins that play an important role in maintaining metal homeostasis and stress response. However, the evolutionary relationships and functional differentiation of in the genus remain unclear. Here we identified 53 genes from six genera, including ssp. , , ssp. , , , and . The genes were clustered into four groups based on phylogenetic analysis. genes are unevenly distributed on chromosomes; almost half of the genes were clustered on chromosome 12, which may result from a fragment duplication containing the genes on chromosome 12. Five pairs of segmental duplication events and ten pairs of tandem duplication events were found in the rice family. The Ka/Ks values of the fifteen duplicated genes indicated that the duplicated genes were under a strong negative selection during evolution. Next, combining the promoter activity assay with gene expression analysis revealed different expression patterns of genes. In addition, the expression of genes was induced under different stresses, including NaCl, CdCl, ABA, and MeJ treatments. Additionally, we found that genes were mainly located in chloroplasts. These results imply that genes play different roles in response to these stresses. All results provide important insights into the evolution of the gene family in the genus, and will be helpful to further study the function of genes.
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http://dx.doi.org/10.3390/ijms22179651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431808PMC
September 2021

A mobile swabbing booth to address Singapore GPs' concerns about swabber protection: human-centred design during the COVID-19 pandemic.

BMC Fam Pract 2021 09 8;22(1):180. Epub 2021 Sep 8.

Lee Kong Chian School of Medicine, Nanyang Technological University, Experimental Medicine Building, 59 Nanyang Drive, Singapore, 636921, Singapore.

Background: During the COVID-19 pandemic, the Ministry of Health asked Singapore's private general practitioners (GPs) to perform swab testing in their clinics, but some GPs had concerns about swabber protection. Our aim was to develop a swabbing booth to address these concerns.

Methods: We developed a prototype with potential GP users using a human-centred design approach and piloted it with 10 GP clinics. The pilot was then extended to 170 GP clinics around Singapore. These GPs were then surveyed on user satisfaction.

Results: Ninety-three GPs (54%) responded. The majority (75%) practiced in public residential estates in small practices (mean 1.95 doctors). 86% requested the booth to enhance swabber protection. 74% "would recommend" or "would strongly recommend" the booth to colleagues. 79% continue to use the booth to conduct swab tests. 92% liked that it offered swabber protection. 71% liked that the booth created a separate space for swabbing and 64% liked its ease of disinfection. 47% started swabbing only after receiving the booth and 58% said the booth was "important" or "very important" to their decision to participate in swab testing. However, 34% disliked that it took up too much space and the most frequently critiqued area was the gloves.

Conclusion: The human-centred design approach generated a product that had high user satisfaction, addressed GPs' concerns of swabber protection and increased GPs' participation in swab testing. The booth may be useful where GPs are concerned about swabber protection and space is limited.
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http://dx.doi.org/10.1186/s12875-021-01531-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425014PMC
September 2021

Erratum: "Distinct chemical fixation of CO enabled by exotic gold nanoclusters" [J. Chem. Phys. 155, 054305 (2021)].

J Chem Phys 2021 Sep;155(9):099901

School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.

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http://dx.doi.org/10.1063/5.0066159DOI Listing
September 2021

Xenon Nanobubbles for the Image-Guided Preemptive Treatment of Acute Ischemic Stroke via Neuroprotection and Microcirculatory Restoration.

ACS Appl Mater Interfaces 2021 Sep 8. Epub 2021 Sep 8.

School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, P. R. China.

Early lesion site diagnosis and neuroprotection are crucial to the theranostics of acute ischemic stroke. Xenon (Xe), as a nontoxic gaseous neuroprotectant, holds great promise for ischemic stroke therapy. In this study, Xe-encapsulated lipid nanobubbles (Xe-NBs) have been prepared for the real-time ultrasound image-guided preemptive treatment of the early stroke. The lipids are self-assembled at the interface of free Xe bubbles, and the mean diameter of Xe-NBs is 225 ± 11 nm with a Xe content of 73 ± 2 μL/mL. The results show that Xe-NBs can protect oxygen/glucose-deprived PC12 cells against apoptosis and oxidative stress. Based on the ischemic stroke mice model, the biodistribution, timely ultrasound imaging, and the therapeutic effects of Xe-NBs for stroke lesions were investigated . The accumulation of Xe-NBs to the ischemic lesion endows ultrasound contrast imaging with the lesion area. The cerebral blood flow measurement indicates that the administration of Xe-NBs can improve microcirculatory restoration, resulting in reduced acute microvascular injury in the lesion area. Furthermore, local delivery of therapeutic Xe can significantly reduce the volume of cerebral infarction and restore the neurological function with reduced neuron injury against apoptosis. Therefore, Xe-NBs provide a novel nanosystem for the safe and rapid theranostics of acute ischemic stroke, which is promising to translate into the clinical management of stroke.
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http://dx.doi.org/10.1021/acsami.1c06014DOI Listing
September 2021

Macrophage monocarboxylate transporter 1 promotes peripheral nerve regeneration after injury in mice.

J Clin Invest 2021 Sep 7. Epub 2021 Sep 7.

Johns Hopkins University and School of Medicine, Baltimore, United States of America.

Peripheral nerves have the capacity for regeneration, but the rate of regeneration is so slow that many nerve injuries lead to incomplete recovery and permanent disability for patients. Macrophages play a critical role in the peripheral nerve response to injury, both for Wallerian degeneration and for contributing to regeneration, and their function has recently been shown to be dependent on intracellular metabolism. To date, the impact of their intracellular metabolism on peripheral nerve regeneration has not been studied. Examining conditional transgenic mice with selective ablation of solute carrier family 16, member 1 (Slc16a1, which encodes the monocarboxylate transporter 1, MCT1) in macrophages, we found that MCT1 contributes to macrophage metabolism, phenotype, and function, specifically in regard to phagocytosis and supporting peripheral nerve regeneration. Adoptive cell transfer of wild-type macrophages ameliorated the impaired nerve regeneration in macrophage-selective MCT1 null mice. We also developed a mouse model that overexpresses MCT1 in macrophages and found that peripheral nerves in these mice regenerated more rapidly than control mice. Our study provides further evidence that MCT1 has an important biological role in macrophages and that manipulations of macrophage metabolism can enhance recovery from peripheral nerve injuries, for which there are currently no approved medical therapies.
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http://dx.doi.org/10.1172/JCI141964DOI Listing
September 2021

Prognosis of Biliary Atresia Associated With Cytomegalovirus: A Meta-Analysis.

Front Pediatr 2021 18;9:710450. Epub 2021 Aug 18.

Department of General Surgery, Tianjin Children's Hospital, Tianjin, China.

The etiology of biliary atresia is unclear, but viral infection has been implicated. The aim of the current meta-analysis was to investigate relationships between cytomegalovirus (CMV) and the prognosis of biliary atresia. PubMed, Embase, the Cochrane Library, the China National Knowledge Infrastructure database, and Wanfang Data electronic databases were searched for eligible studies. Each relevant text was thoroughly reviewed and examined, including related papers in their reference lists. A total of nine studies including 784 patients were included in the analysis. Biliary atresia patients with CMV exhibited significantly lower jaundice clearance (odds ratio: 0.46, < 0.0001; = 15%, = 0.31). There were no significant differences in the rates of cholangitis or native liver survival. CMV-pp65-positive biliary atresia patients had a significantly lower rate of jaundice clearance (odds ratio: 5.87, = 0.003; = 0%, = 0.71) and a significantly higher rate of cholangitis (odds ratio: 0.21, = 0.01; = 0%, = 0.43) than CMV antibody-positive biliary atresia patients. Biliary atresia patients who were also infected with CMV had a poorer prognosis, particularly with respect to jaundice clearance. CMV status may influence the prognosis of biliary atresia. Clinicians should be able to routinely identify the subset of biliary atresia patients who are also CMV-positive, in order to improve native liver survival.
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http://dx.doi.org/10.3389/fped.2021.710450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416545PMC
August 2021

Outcomes of dexmedetomidine sedation for drug-induced sleep ciné magnetic resonance imaging studies in pediatric obstructive sleep apnea patients.

Paediatr Anaesth 2021 Sep 3. Epub 2021 Sep 3.

Department of Anesthesiology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.

Background: Dexmedetomidine is utilized as a sedative agent for drug-induced sleep cine magnetic resonance imaging studies due to its ability to mimic natural sleep and lack of respiratory depressant effects. The outcomes of dexmedetomidine sedation such as respiratory complications and unplanned admissions in obstructive sleep apnea patients undergoing these studies are currently unknown.

Aim: To describe the outcomes of dexmedetomidine sedation for outpatient drug-induced sleep magnetic resonance imaging in pediatric patients with obstructive sleep apnea.

Methods: This is a retrospective chart review conducted in pediatric patients with obstructive sleep apnea undergoing outpatient drug-induced sleep ciné magnetic resonance imaging studies with dexmedetomidine sedation. Demographics, comorbidities, polysomnography study results, vital signs, respiratory complications, airway interventions, successful completion of the scan, and unplanned hospital admissions were measured.

Main Results: We analyzed 337 patients aged 2-18 years (median age of 11 years). The imaging was completed with dexmedetomidine as the sole sedative agent in 61% (N = 207) patients. Ketamine was administered as additional sedative agent in 36% (N = 122) of the patients. There was no difference in sedation-related adverse events and respiratory complications with regard to the severity of sleep apnea with the exception of mild desaturation episodes (SpO2 85%-90%). Patients who received additional sedative agents had significantly longer recovery room stay (71.5 [44] vs 55 [39] minutes; 95% CI of difference [9 to 23 min], p < 0.001) and total periprocedural stay (164.5 [52] vs 138 [64] minutes; 95% CI of difference [17 to 35 min], p < .001).

Conclusions: Dexmedetomidine alone or along with ketamine provided acceptable sedation in majority of the patients with obstructive sleep apnea undergoing outpatient diagnostic sleep magnetic resonance imaging studies without significant respiratory adverse events regardless of the severity of sleep apnea. Sedation failure and unplanned admissions are rare, and routine planned admission may not be required for this patient population.
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http://dx.doi.org/10.1111/pan.14290DOI Listing
September 2021

Bottlebrush Polymer-Conjugated Melittin Exhibits Enhanced Antitumor Activity and Better Safety Profile.

ACS Appl Mater Interfaces 2021 Sep 2. Epub 2021 Sep 2.

Departments of Chemistry and Chemical Biology, Chemical Engineering, and Bioengineering, Northeastern University, Boston, Massachusetts 02115, United States.

Despite potency against a variety of cancers in preclinical systems, melittin (MEL), a major peptide in bee venom, exhibits non-specific toxicity, severe hemolytic activity, and poor pharmacological properties. Therefore, its advancement in the clinical translation system has been limited to early-stage trials. Herein, we report a biohybrid involving a bottlebrush-architectured poly(ethylene glycol) (PEG) and MEL. Termed pacMEL, the conjugate consists of a high-density PEG arrangement, which provides MEL with steric inhibition against protein access, while the high molecular weight of pacMEL substantially enhances plasma pharmacokinetics with a ∼10-fold increase in the area under the curve (AUC) compared to free MEL. pacMEL also significantly reduces hepatic damage and unwanted innate immune response and all but eliminated hemolytic activities of MEL. Importantly, pacMEL passively accumulates at subcutaneously inoculated tumor sites and exhibits stronger tumor-suppressive activity than molecular MEL. Collectively, pacMEL makes MEL a safer and more appealing drug candidate.
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http://dx.doi.org/10.1021/acsami.1c14285DOI Listing
September 2021

Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair.

Bioact Mater 2022 Jan 4;7:126-143. Epub 2021 Jun 4.

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, PR China.

Although with the good biological properties, silk fibroin (SF) is immensely restrained in long-distance vascular defect repair due to its relatively fast degradation and inferior mechanical properties. It is necessary to construct a multifunctional composite scaffold based on SF. In this study, a novel magnetic SF scaffold (MSFCs) was prepared by an improved infiltration method. Compared with SF scaffold (SFC), MSFCs were found to have better crystallinity, magnetocaloric properties, and mechanical strength, which was ascribed to the rational introduction of iron-based magnetic nanoparticles (MNPs). Moreover, and experiments demonstrated that the degradation of MSFCs was significantly extended. The mechanism of delayed degradation was correlated with the dual effect that was the newly formed hydrogen bonds between SFC and MNPs and the complexing to tyrosine (Try) to inhibit hydrolase by internal iron atoms. Besides, the β-crystallization of protein in MSFCs was increased with the rise of iron concentration, proving the beneficial effect after MNPS doped. Furthermore, although macrophages could phagocytose the released MNPs, it did not affect their function, and even a reasonable level might cause some cytokines to be upregulated. Finally, and studies demonstrated that MSFCs showed excellent biocompatibility and the growth promotion effect on CD34-labeled vascular endothelial cells (VECs). In conclusion, we confirm that the doping of MNPs can significantly reduce the degradation of SFC and thus provide an innovative perspective of multifunctional biocomposites for tissue engineering.
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http://dx.doi.org/10.1016/j.bioactmat.2021.04.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379427PMC
January 2022

[Study on the intervention of platelet biological activity by ultrasound].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Aug;38(4):709-715

Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210096, P.R.China.

Platelets are non-nucleated blood effector cells, which plays an important role in coagulation, hemostasis, and thrombosis. However, platelets are extremely susceptible to activation by external stimuli, which in turn damages the platelet's natural biological activity and affects its biological function. Platelet biological activity has become a hotspot in the field of vascular diseases. In this study, ultrasound parameters (ultrasound intensity and duration time) were used to intervene in the biological activity of platelets. The response of platelets to ultrasound energy was explored from the aspects of platelet morphology, aggregation ability and particle release (the expression of P-selectin and the number of particles). The results showed that the ultrasound intensity of 0.25 W/cm (1 MHz, 60 s) had no effect on the morphology, aggregation ability and particle release of platelets. When the ultrasonic intensity was increased to greater than 0.25 W/cm , the generation of platelet pseudopods, morphological changes, increase of particle release, as well as effect on aggregation were observed. When the ultrasound duration time was 60 s (1 MHz, 0.25 W/cm ), it had no effect on the biological activity of platelets. However, when the ultrasound time was greater than 60 s, the morphology, aggregation ability and microparticles release would been induced with no effect on the secretion of CD62P and total protein components. Therefore, when the ultrasound parameters were 1 MHz and 0.25 W/cm with 60 s duration time, the ultrasound energy had no effect on the biological activity of platelets. The results in this study are of great significant for ultrasound energy intervention for the treatment of platelet-related diseases.
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http://dx.doi.org/10.7507/1001-5515.202005036DOI Listing
August 2021

G6pd-Deficient Mice Are Protected From Experimental Cerebral Malaria and Liver Injury by Suppressing Proinflammatory Response in the Early Stage of Infection.

Front Immunol 2021 11;12:719189. Epub 2021 Aug 11.

Department of Cell Biology and Medical Genetics, Kunming Medical University, Kunming, China.

Epidemiological studies provide compelling evidence that glucose-6-phosphate dehydrogenase (G6PD) deficiency individuals are relatively protected against parasite infection. However, the animal model studies on this subject are lacking. Plus, the underlying mechanism is poorly known. In this study, we used a G6pd-deficient mice infected with the rodent parasite () to set up a malaria model in mice. We analyzed the pathological progression of experimental cerebral malaria (ECM) and acute liver injury in mice with different G6pd activity infected with . We performed dual RNA-seq for host-parasite transcriptomics and validated the changes of proinflammatory response in the murine model. G6pd-deficient mice exhibited a survival advantage, less severe ECM and mild liver injury compared to the wild type mice. Analysis based on dual RNA-seq suggests that G6pd-deficient mice are protected from ECM and acute liver injury were related to proinflammatory responses. Th1 differentiation and dendritic cell maturation in the liver and spleen were inhibited in G6pd-deficient mice. The levels of proinflammatory cytokines were reduced, chemokines and vascular adhesion molecules in the brain were significantly down-regulated, these led to decreased cerebral microvascular obstruction in G6pd-deficient mice. We generated the result that G6pd-deficiency mediated protection against ECM and acute liver injury were driven by the regulatory proinflammatory responses. Furthermore, bioinformatics analyses showed that might occur ribosome loss in G6pd-deficient mice. Our findings provide a novel perspective of the underlying mechanism of G6PD deficiency mediated protection against malaria .
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http://dx.doi.org/10.3389/fimmu.2021.719189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386684PMC
August 2021

The apoptosis of grass carp (Ctenopharyngodon idella) muscle during postmortem condition regulated by the cytokines via TOR and NF-κB signaling pathways.

Food Chem 2021 Aug 19;369:130911. Epub 2021 Aug 19.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Postmortem alteration by apoptosis has significant effects on flesh quality. Currently, the information necessary to understand the apoptotic behavior and the molecular mechanisms during postmortem alteration in fish muscle is still lacking. Activation of apoptosis and the cytokines involved in regulating apoptosis in fish muscle were evaluated during postmortem condition at 4 °C for 5 days in terms of apoptotic morphology changes, nucleus DNA fragmentation, caspases activation and related gene expressions. The triggering apoptotic mechanisms associated with multiple cytokines transcriptional levels showed that the up-regulated pro-apoptotic mediators [IFN-γ2, TNF-α, IL-6, IL-1β, IL-17D, IL-12p35 and IL-10 (except IL-15)] and the down-regulated anti-apoptotic mediators of [IL-8 and IL-11 (except TGF-β and IL-4)] both regulated apoptosis at early stage, which were regulated by NF-κB and TOR, respectively. Results suggested that transcriptional regulation of multiple cytokines produce a positive outcome on triggering apoptosis.
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http://dx.doi.org/10.1016/j.foodchem.2021.130911DOI Listing
August 2021

Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard ().

Animals (Basel) 2021 Aug 21;11(8). Epub 2021 Aug 21.

School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Vertebrates maintain complex symbiotic relationships with microbiota living within their gastrointestinal tracts which reflects the ecological and evolutionary relationship between hosts and their gut microbiota. However, this understanding is limited in lizards and the spatial heterogeneity and co-occurrence patterns of gut microbiota inside the gastrointestinal tracts of a host and variations of microbial community among samples remain poorly understood. To address this issue and provide a guide for gut microbiota sampling from lizards, we investigated the bacteria in three gut locations of the oriental garden lizard () and the data were analyzed for bacterial composition by 16S ribosomal RNA (16S rRNA) gene amplicon sequencing. We found the relative abundance of the dominant phyla exhibited an increasing trend from the small intestine to the large intestine, and phyla , and were the three primary phyla in the gut microbiota of . There were a higher abundance of genus (Class: ), and (Class: ), (Family: ) and (Family: ), and Family and in the sample from the hindgut. The secondary bile acid biosynthesis, glycosaminoglycan degradation, sphingolipid metabolism and lysosome were significantly higher in the hindgut than that in the small intestine. Taken together our results indicate variations of gut microbiota composition and metabolic pathway in different parts of the oriental garden lizard.
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http://dx.doi.org/10.3390/ani11082461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388656PMC
August 2021

Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.

J Cell Sci 2021 01 27;134(2). Epub 2021 Jan 27.

School of Public Health, Medical Research Center, Hebei Key Laboratory for Organ Fibrosis Research, North China University of Science and Technology, Tangshan 063210, China.

Silicosis is characterized by silica exposure-induced lung interstitial fibrosis and formation of silicotic nodules, resulting in lung stiffening. The acetylation of microtubules mediated by α-tubulin N-acetyltransferase 1 (α-TAT1) is a posttranslational modification that promotes microtubule stability in response to mechanical stimulation. α-TAT1 and downstream acetylated α-tubulin (Ac-α-Tub) are decreased in silicosis, promoting the epithelial-mesenchymal transition (EMT); however, the underlying mechanisms are unknown. We found that silica, matrix stiffening or their combination triggered Ac-α-Tub downregulation in alveolar epithelial cells, followed by DNA damage and replication stress. α-TAT1 elevated Ac-α-Tub to limit replication stress and the EMT via trafficking of p53-binding protein 1 (53BP1, also known as TP53BP1). The results provide evidence that α-TAT1 and Ac-α-Tub inhibit the EMT and silicosis fibrosis by preventing 53BP1 mislocalization and relieving DNA damage. This study provides insight into how the cell cycle is regulated during the EMT and why the decrease in α-TAT1 and Ac-α-Tub promotes silicosis fibrosis. This article has an associated First Person interview with the first authors of the paper.
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http://dx.doi.org/10.1242/jcs.243394DOI Listing
January 2021

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis.

Oxid Med Cell Longev 2021 14;2021:4158495. Epub 2021 Aug 14.

Basic Medical College, Hebei Key Laboratory for Chronic Diseases, North China University of Science and Technology, Tangshan, Hebei Province 063210, China.

Cellular senescence has been considered an important driver of many chronic lung diseases. However, the specific mechanism of cellular senescence in silicosis is still unknown. In the present study, silicotic rats and osteoclast stimulatory transmembrane protein () overexpression of MLE-12 cells were used to explore the mechanism of OC-STAMP in cellular senescence in alveolar epithelial cell type II (AEC2). We found an increasing level of OC-STAMP in AEC2 of silicotic rats. Overexpression of in MLE-12 cells promoted epithelial-mesenchymal transition (EMT), endoplasmic reticulum (ER) stress, and cellular senescence. Myosin heavy chain 9 (MYH9) was a potential interacting protein of OC-STAMP. Knockdown of or inhibited cellular senescence in MLE-12 cells transfected with pcmv6-. Treatment with 4-phenylbutyrate (4-PBA) to inhibit ER stress also attenuated cellular senescence or . In conclusion, OC-STAMP promotes cellular senescence in AEC2 in silicosis.
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http://dx.doi.org/10.1155/2021/4158495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380176PMC
August 2021

Genetic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Guangzhou, China.

Virol J 2021 08 23;18(1):174. Epub 2021 Aug 23.

The Key Laboratory for Virology of Guangzhou, Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, China.

Background: Human rhinovirus (HRV) is one of the major viruses of acute respiratory tract disease among infants and young children. This work aimed to understand the epidemiological and phylogenetic features of HRV in Guangzhou, China. In addition, the clinical characteristics of hospitalized children infected with different subtype of HRV was investigated.

Methods: Hospitalized children aged < 14 years old with acute respiratory tract infections were enrolled from August 2018 to December 2019. HRV was screened for by a real-time reverse-transcription PCR targeting the viral 5'UTR.

Results: HRV was detected in 6.41% of the 655 specimens. HRV infection was frequently observed in children under 2 years old (57.13%). HRV-A and HRV-C were detected in 18 (45%) and 22 (55%) specimens. All 40 HRV strains detected were classified into 29 genotypes. The molecular evolutionary rate of HRV-C was estimated to be 3.34 × 10 substitutions/site/year and was faster than HRV-A (7.79 × 10 substitutions/site/year). Children who experienced rhinorrhoea were more common in the HRV-C infection patients than HRV-A. The viral load was higher in HRV-C detection group than HRV-A detection group (p = 0.0148). The median peak symptom score was higher in patients with HRV-C infection as compared to HRV-A (p = 0.0543), even though the difference did not significance.

Conclusion: This study revealed the molecular epidemiological characteristics of HRV in patients with respiratory infections in southern China. Children infected with HRV-C caused more severe disease characteristics than HRV-A, which might be connected with higher viral load in patients infected with HRV-C. These findings will provide valuable information for the pathogenic mechanism and treatment of HRV infection.
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http://dx.doi.org/10.1186/s12985-021-01645-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382100PMC
August 2021

The Mechanisms of Sevoflurane-Induced Neuroinflammation.

Front Aging Neurosci 2021 5;13:717745. Epub 2021 Aug 5.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Sevoflurane is one of the most commonly used inhaled anesthetics due to its low blood gas coefficient, fast onset, low airway irritation, and aromatic smell. However, recent studies have reported that sevoflurane exposure may have deleterious effects on cognitive function. Although neuroinflammation was most widely mentioned among the established mechanisms of sevoflurane-induced cognitive dysfunction, its upstream mechanisms have yet to be illustrated. Thus, we reviewed the relevant literature and discussed the most mentioned mechanisms, including the modulation of the microglial function, blood-brain barrier (BBB) breakdown, changes in gut microbiota, and ease of cholinergic neurotransmission to help us understand the properties of sevoflurane, providing us new perspectives for the prevention of sevoflurane-induced cognitive impairment.
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http://dx.doi.org/10.3389/fnagi.2021.717745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375153PMC
August 2021

Influence of epileptogenic region on brain structural changes in Rolandic epilepsy.

Brain Imaging Behav 2021 Aug 22. Epub 2021 Aug 22.

Department of Medical Imaging, Jinling Hospital, Nanjing Clinical School, Southern Medical University, Nanjing, 210002, China.

To investigate the influence of epileptogenic cortex (Rolandic areas) with executive functions in Rolandic epilepsy using structural covariance analysis of structural magnetic resonance imaging (MRI). Structural MRI data of drug-naive patients with Rolandic epilepsy (n = 70) and typically developing children as healthy controls (n = 83) were analyzed using voxel-based morphometry. Gray matter volumes in the patients were compared with those of healthy controls, and were further correlated with epilepsy duration and cognitive score of executive function, respectively. By applying Granger causal analysis to the sequenced morphometric data according to disease progression information, causal network of structural covariance was constructed to assess the causal influence of structural changes from Rolandic cortices to the regions engaging executive function in the patients. Compared with healthy controls, epilepsy patients showed increased gray matter volume in the Rolandic regions, and also the regions engaging in executive function. Covariance network analyses showed that along with disease progression, the Rolandic regions imposed positive causal influence on the regions engaging in executive function. In the patients with Rolandic epilepsy, epileptogenic regions have causal influence on the structural changes in the regions of executive function, implicating damaging effects of Rolandic epilepsy on human brain.
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http://dx.doi.org/10.1007/s11682-021-00517-5DOI Listing
August 2021

China experts' consensus on preventive and interceptive orthodontic treatments of malocclusions of children.

Hua Xi Kou Qiang Yi Xue Za Zhi 2021 Aug;39(4):369-376

Wuxi Stomatology Hospital, Wuxi 214000, China.

Malocclusion is one of the three most common oral diseases reported by World Health Organization(WHO). In China, its incidence rate is rising. Malocclusion seriously affects the dental and maxillofacial function, facial appearance and growth development of nearly 260 million children in China, and what is more, it affects their physical and mental health development. Malocclusion occurrence is related to genetic and environmental factors. Early treatment of malocclusion can create a good dental and maxillofacial development environment, correct abnormal growth and control the adverse effects of abnormal genetic factors. It can effectively reduce the prevalence of children's malocclusion and enhance their physical and mental health. This is an urgent need from the economic perspective of our society, so it has great practical and social significance. Experts from the project group "standard diagnose and treatment protocols for early orthodontic intervention of malocclusions of children" which initiated by China National Health Institute of Hospital Administration wrote the "China Experts' Consensus on Preventive and Interceptive Orthodontic Treatments of Malocclusions of Children", which aims to guide and popularize the clinical practice, improve the clinical theory and practice level, and accelerate the disciplinary development of early treatment of children's malocclusion in China. The consensus elaborates the harmfulness of malocclusion and the necessity of early treatment, and brings up the principles and fundamental contents. Based on the law of dental and maxillofacial development, this paper puts forward the guiding suggestions of preventive and interceptive treatments in different stages of dental development ranging from fetus to early permanent dentition. It is a systematic project to promote and standardize the early treatment of malocclusion. Through scientific and comprehensive stratified clinical practice and professional training, the clinical system of early treatment of malocclusion in China will eventually be perfected, so as to comprehensively care for children's dental and maxillofacial health, and improve their oral and physical health in China.
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http://dx.doi.org/10.7518/hxkq.2021.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381117PMC
August 2021

Research on clinical curative effect of small incision closure through the chest of ventricular septal defect (VSD) in children.

Asian J Surg 2021 Aug 14. Epub 2021 Aug 14.

Department of Thoracic Surgery, Children's Hospital Affiliated to Zhengzhou University; Henan Provincial Children's Hospital; Zhengzhou Children's Hospital, Zhengzhou, 450007, China.

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http://dx.doi.org/10.1016/j.asjsur.2021.07.014DOI Listing
August 2021

Theoretical study of the effects of nanoparticles on the acoustic performance of microbubbles.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Aug 17;PP. Epub 2021 Aug 17.

There are growing investigations on incorporating solid nanoparticles (NPs) into the shell of microbubbles (MBs), because NPs may endow the MBs with other bio-functions, such as multimodality imaging and drug delivery. These novel MBs have been developed as hybrid MBs contrast agents. Generally, the shell density of hybrid MBs was assumed to be the same as water in the studies of bubble dynamics. In fact, the NPs in the layer of MBs can change the density of the shell, which leads to the change of scattering characteristics of MBs under ultrasonic excitation. Thus, it is necessary to develop a new model to simulate dynamics of the hybrid MBs. Here, we have investigated scattering characteristics of the hybrid MB embedded with NPs based on a modified Rayleigh-Plesset model. The numerical and analytical solutions to this equation are obtained for oscillation response, harmonic-components and scattered cross section of hybrid MB at small amplitude oscillations. The results indicated that the shell density had a greater impact on the nonlinear harmonics than fundamental ones. Considering acoustic driving frequency and pulse lengths, the largest sub-harmonic amplitude is 14 times larger than the smallest value. Considering the effects of bubble equilibrium radius, the second scattering cross-section of hybrid MB increased first and then decreased with increasing bubble equilibrium radius. Therefore, the optimal values of shell density for hybrid MB can be predicted to obtain higher scattered signals. This also offers more accurate assessment of scattering characteristics for hybrid MB contrast agents.
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http://dx.doi.org/10.1109/TUFFC.2021.3105430DOI Listing
August 2021

Long-term follow-up results of PTMC treated by ultrasound-guided radiofrequency ablation: a retrospective study.

Int J Hyperthermia 2021 ;38(1):1225-1232

Department of Ultrasound, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

Objectives: To confirm the long-term efficacy and safety of radiofrequency ablation (RFA) for low-risk papillary thyroid microcarcinoma (PTMC).

Methods: We retrospectively reviewed data of 102 primary papillary thyroid carcinoma patients (82 women, 20 men; mean age: 43 [19] years) treated with radiofrequency ablation and thyroid-stimulating hormone (TSH) suppression therapy before December 2018. All patients were at high surgical risk or refused surgery. They were followed up at 1, 3, 6, 9, and 12 months and every 6-12 months thereafter using ultrasound and contrast-enhanced ultrasound. The volume and volume reduction ratio was calculated. Recurrence and lymph node or distant metastasis were evaluated.

Results: The mean initial tumor diameter was 0.50 (0.29) cm; the mean initial volume was 0.06 (0.09) mL. At 1, 3, 6, 9, 12, 24, 36, 48, and 60 months after RFA, complete resorption rates were 0, 0, 9.8 (10/102), 33.3 (34/102), 91.2 (93/102), 96.1 (98/102), 99 (101/102), 100, and 100%, respectively. Two patients had developed ipsilateral neck lymph node metastasis in regions IV and VI at 30- and 18-month follow-ups, respectively. After RFA, 3/102 patients (2.9%) developed hoarseness-the main side effect. No life-threatening or delayed complications occurred. The TSH value in the initial period was 0.06 (0.02) µIU/mL; the rate of reaching the TSH target was 85.7%. The TSH value at follow-up was 1.47 (0.91) µIU/mL; the compliance rate was 99.3%.

Conclusions: Ultrasound-guided RFA for PTMC is highly effective and safe. RFA can serve as a minimally invasive treatment for PTMC patients who refuse surgery or active surveillance.
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http://dx.doi.org/10.1080/02656736.2021.1963850DOI Listing
September 2021

Pragmatic comparative effectiveness study of multimodal fascia iliaca nerve block and continuous lumbar epidural-based protocols for periacetabular osteotomy.

J Hip Preserv Surg 2020 Dec 26;7(4):728-739. Epub 2021 Feb 26.

Department of Anesthesiology, Cincinnati Children's Hospital, Cincinnati, OH, USA.

Perioperative pain management protocols have a significant impact on early surgical outcomes and recovery. We hypothesized that multimodal protocol including fascia iliaca compartment nerve block (MM-FICNB) would decrease the length of hospital stay (LOS) by facilitating earlier mobilization, without compromising analgesia, compared to a traditional lumbar epidural-based protocol (EP). Demographics/comorbidities, surgical/block characteristics and perioperative pain/mobilization data were collected from a prospectively recruited MM-FICNB group ( = 16) and a retrospective EP cohort ( = 16) who underwent PAO using similar surgical techniques, physical therapy/discharge criteria. Association of MM-FICNB group with LOS (primary outcome), postoperative pain, postoperative opioid requirements in morphine equivalent rates (MER) (mcg/kg/h) and time to complete physical therapy were tested using multivariable and survival regression. Patient and surgical characteristics were similar between groups. Median time for FICNB performance was significantly less than epidural (6 versus 15 min;  < 0.001). LOS was significantly decreased in the MM-FICNB group (2.88 ± 0.72 days) compared to the EP group (4.38 ± 1.02 days);  < 0.001. MM-FICNB group had significantly lower MER on POD1 ( = 0.006) and POD2 ( < 0.001), with similar pain scores on all POD. MM-FICNB group was associated with decreased LOS and earlier mobilization ( < 0.001) by covariate-adjusted multivariate regression. Cox proportional hazard regression model showed MM-FICNB subjects had 63 (95% CI 7-571,  < 0.001) times the chance of completing physical therapy goals, compared to EP. Compared to EP, MM-FICNB protocol allowed earlier mobilization and decreased post-surgical hospitalization by 1.5 days, without compromising analgesia, with important implications for value-based healthcare and cost-effectiveness.
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http://dx.doi.org/10.1093/jhps/hnab010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349585PMC
December 2020

[Retracted] MicroRNA‑101 induces apoptosis in cisplatin‑resistant gastric cancer cells by targeting VEGF‑C.

Mol Med Rep 2021 Oct 9;24(4). Epub 2021 Aug 9.

Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University,Weihui, Henan 453100, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the cell apoptotic assay data shown in Figs. 3C and 5B were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 13: 572‑578, 2016; DOI: 10.3892/mmr.2015.4560].
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http://dx.doi.org/10.3892/mmr.2021.12333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365414PMC
October 2021

Facile construction of novel organic-inorganic tetra (4-carboxyphenyl) porphyrin/BiMoO heterojunction for tetracycline degradation: Performance, degradation pathways, intermediate toxicity analysis and mechanism insight.

J Colloid Interface Sci 2021 Jul 30;605:727-740. Epub 2021 Jul 30.

Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, National Engineering Research Center for Marine Aquaculture, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang Province, 316022, China. Electronic address:

Developing durable photocatalysts with highly efficient antibiotics degradation is crucial for environment purification. Herein, tetra (4-carboxyphenyl) porphyrin (TCPP) was loaded onto the surface of BiMoO microspheres to gain hierarchical organic-inorganic TCPP/BiMoO (TCPP/BMO) heterojunctions via a facile impregnation strategy. The catalytic properties of these catalysts were comprehensively investigated through the photodegradation of tetracycline hydrochloride (TC) under visible light. Among all the TCPP/BMO heterojunctions, the highest photodegradation rate constant (0.0278 min) was achieved with 0.25 wt% TCPP (TCPP/BMO-2), which was approximately 1.15 folds greater than that of pristine BiMoO and far superior to pure TCPP. The extremely high photocatalytic performance is attributed to the interfacial interaction between TCPP and BiMoO, which favors the efficient separation of charge carriers and the enhancement of visible-light absorbance. TCPP/BMO-2 possesses high mineralization capability and good recycling performance. Photo-induced O, h, and OH were mainly responsible for the degradation of TC. The degradation pathways of TC and toxicity of degradation intermediates were analyzed based on the intermediates detected by the high performance liquid chromatography-mass spectrometer (HPLC-MS) and the toxicity assessment by the quantitative structure-activity relationship (QSAR) prediction. A possible photocatalytic mechanism over TCPP/BMO is proposed. This work offers an insight in developing the porphyrin-based organic-inorganic heterojunctions for effectively remedying pharmaceutical wastewater.
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http://dx.doi.org/10.1016/j.jcis.2021.07.137DOI Listing
July 2021

Distinct chemical fixation of CO enabled by exotic gold nanoclusters.

J Chem Phys 2021 Aug;155(5):054305

School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.

Atomically precise metal nanoclusters, especially the metal nanoclusters with an exotic core structure, have given rise to a great deal of interest in catalysis, attributing to their well-defined structures at the atomic level and consequently unique electronic properties. Herein, the catalytic performances of three gold nanoclusters, such as AuS(S-Adm) with a body-centered cubic (bcc) kernel structure, Au(S-Adm) with a hexagonal close-packed (hcp) core structure, and Au(S-Adm) with a face-centered cubic (fcc) kernel structure, were attempted for the CO cycloaddition with epoxides toward cyclic carbonates. Due to the excess positive charge with a strong Lewis acidity and large chemical adsorption capacity, the bcc-AuS(S-Adm) nanocluster outperformed the hcp-Au(S-Adm) and fcc-Au(S-Adm) nanoclusters. Additionally, the synergistic effect between the gold nanocluster and co-catalyst played a crucial role in CO cycloaddition.
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http://dx.doi.org/10.1063/5.0055803DOI Listing
August 2021

[Alternative Splicing Analysis of LACTB Gene and Expression Characteristics of Different Transcripts in Leukemia Cell Lines].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Aug;29(4):1019-1027

Department of Clinical Hematology, School of Medical Laboratory Science Guizhou Medical University, Guiyang 550004, Guizhou Province, China.

Objective: To detect the expression of different transcripts of lactamase β(LACTB) gene in leukemic cell lines.

Methods: NCBI website and DNAstar software were used to detect the Bioinformatics analysis of LACTB. The expression of different transcripts of LACTB gene in leukemic cell lines (THP-1, HL60, K562, U937, Jurkat and Raji) was detected by reverse transcription PCR (RT-PCR), DNA and clone sequencing; the expression of different transcripts of LACTB gene in leukemic cell lines was detected by Quantitative Real-time PCR.

Results: There were a variety of splicing isomers in LACTB, and it could produce a variety of protein isomers with conserved N-terminal and different C-terminal, moreover, there were many splice isoforms of LACTB in leukemia cell lines, and there were different expression patterns in different cell lines, including XR1, V1, V2 and V3. The expression of total LACTB showed high in HL60 cells, while low in Raji cells, and the difference was statistically significant (P<0.05). The V1 was high expression in U937 cells but low in Raji cells, and the difference was statistically significant (P<0.05). V2 was high expression in HL60 cells but lowly in Raji cells, and the difference was statistically significant (P<0.05). The expression of V3 was low in THP-1 cells, which was significantly different as compared with that in normal bone marrow (P<0.05).

Conclusion: The reaserch found that there are many splice isomers of LACTB in leukemic cell lines, and there are different expression patterns in different cell lines.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.04.002DOI Listing
August 2021

Paraquat promotes acute lung injury in rats by regulating alveolar macrophage polarization through glycolysis.

Ecotoxicol Environ Saf 2021 Oct 2;223:112571. Epub 2021 Aug 2.

Department of Emergency Medicine, The First Affiliated Hospital of Zhengzhou University, Henan Key Laboratory of Emergency and Trauma Research Medicine, Zhengzhou 450000, China. Electronic address:

The present study investigates whether paraquat (PQ) regulates polarization of alveolar macrophages through glycolysis and promotes the occurrence of acute lung injury in rats. In vivo, the PQ intraperitoneal injection was used to construct a model of acute lung injury in rats. In vitro, the study measured the effect of different concentrations of PQ on the viability of the alveolar macrophages, and explored the polarization and glycolysis metabolism of alveolar macrophages at different time points after PQ intervention. Compared with the normal control (NC) group, the lung pathological damage in rats increased gradually after PQ poisoning, reaching a significant degree at 48 h after poisoning. The PQ-poisoned rat serum showed increased expressions of interleukin-6 (IL-6), tumor necrosis factor- α (TNF-α), and M1 macrophage marker, iNOS, while the expression of interleukin-10 (IL-10) and M2 macrophage marker, Arg1, decreased. The toxic effect of PQ on alveolar macrophages was dose- and time-dependent. Compared with the NC group, IL-6 and TNF-α in the cell supernatant gradually increased after PQ intervention, while the IL-10 content gradually decreased. The PQ intervention in alveolar macrophages increased the expression of intracellular glycolysis rate-limiting enzyme pyruvate kinase isozymes M1/M2 (PKM1/M2), lactate, lactate/pyruvate ratio, and the polarization of alveolar macrophage towards M1. Inhibition of cellular glycolysis significantly reduced the PQ-induced alveolar macrophage polarization to M1 type. Thus, PQ induced increased polarization of lung macrophages toward M1 and decreased polarization toward M2, promoting acute lung injury. Therefore, it can be concluded that PQ regulates the polarization of alveolar macrophages through glycolysis.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112571DOI Listing
October 2021
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